Membre

@article{oforiagyemang:hal-04362740,

title = {Plant testing with hemp and miscanthus to assess phytomanagement options including biostimulants and mycorrhizae on a metal-contaminated soil to provide biomass for sustainable biofuel production},

author = {Felix Ofori-Agyemang and Christophe Waterlot and James Manu and Roman Laloge and Romain Francin and Eleni Papazoglou and Efthymia Alexopoulou and Anissa Lounès-Hadj Sahraoui and Benoît Tisserant and Michel Mench and Aritz Burges and Nadège Oustrière},

url = {https://hal.science/hal-04362740},

doi = {10.1016/j.scitotenv.2023.169527},

year  = {2024},

date = {2024-02-01},

urldate = {2024-02-01},

journal = {Science of the Total Environment},

volume = {912},

pages = {169527},

publisher = {Elsevier},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{waterlot:hal-04403673,

title = {Sustainable pathways to oxicams via heterogeneous biosourced catalysts - Recyclable and reusable materials},

author = {Christophe Waterlot and Alina Ghinet and Pierrick Dufrénoy and Marie Hechelski and Adam Daïch and Damien Betrancourt and David Bulteel},

url = {https://hal.science/hal-04403673},

doi = {10.1016/j.jclepro.2024.140684},

year  = {2024},

date = {2024-01-01},

urldate = {2024-01-01},

journal = {Journal of Cleaner Production},

pages = {140684},

publisher = {Elsevier},

note = {ACL},

keywords = {ER2, ER4},

pubstate = {published},

tppubtype = {article}

}

@article{OConnor2023,

title = {Trees in temperate alley-cropping systems develop deep fine roots 

	5 years after plantation: What are the consequences on soil resources?},

author = {Claire O'Connor and Bernarhd Zeller and Caroline Choma and François Delbende and Ali Siah and Christophe Waterlot and Kasaina-Sitraka Andrianarisoa},

year  = {2023},

date = {2023-01-01},

journal = {Agriculture, Ecosystems & Environment},

volume = {345},

pages = {108339},

abstract = {Trees in alley-cropping systems (AC) were reported to develop deeper fine roots compared to forest trees and that they can modify soil water (SWC), mineral nitrogen (SMN) and organic matter (SOM) content. However, intercropping young trees has not been studied extensively. This study aimed to count tree fine roots abundance (TFRA) along a chronosequence of AC stands, to determine factors explaining its variability and to highlight its effects on soil resources. Seventeen alley-cropping plots ranging from 3 to 12 years old were chosen on farms in northern France. TFRA was measured by the core break method using soil samples collected at 0, 1, 3 and 10 m from a referent tree (a maple, a hybrid walnut or a hornbeam) down to 2 m depth. Before four years old, tree fine roots colonized the topsoil (0–30 cm) in rows and then mainly grew vertically from 4 to 6 years old, before laterally exploring deep soil layer (1–2 m) beyond this age. Stepwise analyses showed that stand age, tillage frequency and crop rotation duration explained 60 % of the variability of the sum of TFRA calculated for all soil layers at all distances from the tree row. The SWC was negatively correlated to TFRA suggesting that as trees get older, they dried the deep soil layer below the crop rooting zone and increased the soil depth able to store autumn and winter rainfall. No significant effect of either stand age or distance from tree rows was observed for SMN. It varied significantly with soil depth (R² = 0.3***) and was strongly correlated with soil nitrate content (R² = 0.97***). The soil ammonium content was significantly correlated with TFRA, suggesting that tree fine roots favor ammonium production or accumulation in soil, which may potentially allow for a reduction in the mineral nitrogen (N) mobility for leaching. Finally, we found a significantly high SOM correlated with TFRA only in topsoil on the tree rows at our oldest stands. No change of SOM was observed in the deep soil layer regardless of stand age. From this study, we concluded that fine root plasticity of intercropped trees occurred at early stage and may contribute with age to a better use of soil water, to managing the soil mineral N dynamic and to sequestrating carbon, at least in tree rows.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Paguet2023,

title = {Multivariate analysis of chemical and genetic diversity of wild Humulus 

	lupulus L. (hop) collected in situ in northern France},

author = {Anne-Sophie Paguet and Ali Siah and Gabriel Lefèvre and Sophie Moureu and Thierry Cadalen and Jennifer Samaillie and Franck Michels and Barbara Deracinois and Christophe Flahaut and Harmony Alves-Dos-Santos and Audrey Etienne-Debaecker and Caroline Rambaud and Sylvie Chollet and Roland Molinié and Jean-Xavier Fontaine and Christophe Waterlot and Marie-Laure Fauconnier and Sevser Sahpaz and Céline Rivière},

year  = {2023},

date = {2023-01-01},

journal = {Phytochemistry},

volume = {225},

pages = {113508},

abstract = {Highlights •50 wild hops from the North of France were genetically and chemically characterized. •Microsatellite-based study underlined high polymorphism among wild hop accessions. •Volatile compound analysis showed minor but original metabolites in wild hops. •Wild hops differ from commercial varieties in their high β-acid content and metabolomic diversity. •Multifactorial analysis suggested a potential terroir effect to explain this chemodiversity. The hop plant (Humulus lupulus L.) has been exploited for a long time for both its brewing and medicinal uses, due in particular to its specific chemical composition. These last years, hop cultivation that was in decline has been experiencing a renewal for several reasons, such as a craze for strongly hopped aromatic beers. In this context, the present work aims at investigating the genetic and chemical diversity of fifty wild hops collected from different locations in Northern France. These wild hops were compared to ten commercial varieties and three heirloom varieties cultivated in the same sampled geographical area. Genetic analysis relying on genome fingerprinting using 11 microsatellite markers showed a high level of diversity. A total of 56 alleles were determined with an average of 10.9 alleles per locus and assessed a significant population structure (mean pairwise FST = 0.29). Phytochemical characterization of hops was based on volatile compound analysis by HS-SPME GC-MS, quantification of the main prenylated phenolic compounds by UHPLC-UV as well as untargeted metabolomics by UHPLC-HRMS and revealed a high level of chemical diversity among the assessed wild accessions. In particular, analysis of volatile compounds revealed the presence of some minor but original compounds, such as aromadendrene, allo-aromadendrene, isoledene, β-guaiene, α-ylangene and β-pinene in some wild accessions; while analysis of phenolic compounds showed high content of β-acids in these wild accessions, up to 2.37% of colupulone. Genetic diversity of wild hops previously observed was hence supported by their chemical diversity. Sample soil analysis was also performed to get a pedological classification of these different collection sites. Results of the multivariate statistical analysis suggest that wild hops constitute a huge pool of chemical and genetic diversity of this species.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Vuillemin2023,

title = {Copper-uptake mediated by an ecofriendly zwitterionic ionic liquid: 

	A new challenge for a cleaner bioeconomy},

author = {Marie-E. Vuillemin and Christophe Waterlot and Anthony Verdin and Sylvain Laclef and Christine Cézard and David Lesur and Catherine Sarazin and Dominique Courcot and Caroline Hadad and Eric Husson and Albert-Nguyen Van-Nhien},

year  = {2023},

date = {2023-01-01},

journal = {Journal of Environmental Sciences},

volume = {130},

pages = {92-101},

abstract = {This study aims to investigate the ability of an imidazolium biobased Zwitterionic Ionic Liquids (ZILs) in enhancing the phytoavailability of copper from garden (G) and vineyard (V) soils using the model plant ryegrass. Uncontaminated and artificially contaminated CuSO4 soils, unamended and ZIL-amended soil modalities were designed. The copper/ZIL molar ratio (1/4) introduced was rationally established based on molecular modeling and on the maximal copper concentration in artificially contaminated soil. Higher accumulation of copper in the shoots was detected for the uncontaminated and copper contaminated ZIL amended V soils (18.9 and 23.3 mg.kg−1, respectively) contrary to G soils together with a ZIL concentration of around 3% w/w detected by LC-MS analyses. These data evidenced a Cu-accumulation improvement of 38 and 66% compared to non-amended V soils (13.6 and 13.9 mg.kg−1 respectively). ZIL would be mainly present under Cu(II)-ZIL4 complexes in the shoots. The impact on the chemical composition of shoot were also studied. The results show that depending on the soils modalitity, the presence of free copper and/or ZIL led to different chemical compositions in lignin and monomeric sugar contents. In the biorefinery context, performances of enzymatic hydrolysis of shoots were also related to the presence of both ZIL and copper under free or complex forms. Ecotoxicity assessment of the vineyard soil samples indicated that the quantity of copper and ZIL remaining in the soils had no significant toxicity. ZIL amendment in a copper-contaminated soil was demonstrated as being a promising way to promote the valorization of phytoremediation plants.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{oconnor:hal-04288814,

title = {Trees in temperate alley-cropping systems develop deep fine roots 5 years after plantation: What are the consequences on soil resources?},

author = {Claire O'Connor and Bernarhd Zeller and Caroline Choma and François Delbende and Ali Siah and Christophe Waterlot and Kasaina Sitraka Andrianarisoa},

url = {https://hal.inrae.fr/hal-04288814},

doi = {10.1016/j.agee.2022.108339},

year  = {2023},

date = {2023-04-01},

urldate = {2023-04-01},

journal = {Agriculture, Ecosystems & Environment},

volume = {345},

pages = {108339},

publisher = {Elsevier},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{guerin:hal-03771356,

title = {The phytoextraction power of Cichorium intybus L. on metal-contaminated soil: Focus on time- and cultivar-depending accumulation and distribution of cadmium, lead and zinc},

author = {Théo Guérin and Alina Ghinet and Christophe Waterlot},

url = {https://hal.archives-ouvertes.fr/hal-03771356},

doi = {10.1016/j.chemosphere.2021.132122},

year  = {2022},

date = {2022-01-01},

urldate = {2022-01-01},

journal = {Chemosphere},

volume = {287},

pages = {132122},

publisher = {Elsevier},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{luyckx:hal-03851601b,

title = {Impact of Silicon and Heavy Metals on Hemp (Cannabis sativa L.) Bast Fibres Properties: An Industrial and Agricultural Perspective},

author = {Marie Luyckx and Mathilde Blanquet and Arnaud Isenborghs and Gea Guerriero and Géraldine Bidar and Christophe Waterlot and Francis Douay and Stanley Lutts},

url = {https://hal.archives-ouvertes.fr/hal-03851601},

doi = {10.1007/s41742-022-00446-1},

year  = {2022},

date = {2022-10-01},

urldate = {2022-10-01},

journal = {International Journal of Environmental Research},

volume = {16},

number = {5},

pages = {82},

publisher = {Springer Verlag},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Dufrenoy2022,

title = {Green synthesis of a new series of pyroglutamides targeting human 

	farnesyltransferase},

author = {Pierrick Dufrénoy and Marie Hechelski and Adam Daïch and Amaury Farce and Emmanuelle Lipka and Joëlle Dubois and Christophe Waterlot and Alina Ghinet},

year  = {2022},

date = {2022-01-01},

journal = {Sustainable Chemistry and Pharmacy},

volume = {30},

pages = {100894},

abstract = {Pyroglutamide derivatives have emerged as promising inhibitors of human farnesyltransferase (FTIs), an important target in oncology and also in rare diseases such as Hutchinson-Gilford progeria syndrome (HGPS). This report describes the chemical efforts to enrich the pyroglutamide series using greener and recyclable catalysts. The central reaction studied was an amidation between methyl pyroglutamates or vinylogues and amines. Ten catalysts have been tested in this amidation reaction: two classical Lewis acids (ZnCl2, ZrCl4), four impregnated montmorillonite K10 with ZnCl2 namely Cat1, Cat2, Cat3 and Cat4 (not activated, activated at 120 °C, 280 °C and 500 °C, respectively) and four montmorillonites K10 (commercial montmorillonite K10 not activated, activated at 120 °C, 280 °C and 500 °C). The most efficient catalyst was Cat4. The recyclability of Cat4 over five synthesis runs has been successfully tested. Twenty-six amides were synthesized and screened for their potential to inhibit human farnesyltransferase. Four points of chemical modulation around the pyrrolidine-2-one ring have been realized allowing to complete structure-activity relationships in these series. The study revealed several potent inhibitors targeting human farnesyltransferase in vitro with IC50 values in the submicromolar range and down to 30 nM. The docking of compounds in the active site of FTase highlighted that the S-isomers of pyroglutamides had good affinity. This study propels pyroglutamide derivatives as promising candidates for future functionality assays and in vivo evaluation.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Janus2022,

title = {Synthesis of bio-sourced catalysts from ryegrass produced on the 

	metallurgical sites for the synthesis of therapeutic molecules: a 

	possible option? Feedback from the NEW-REGENERATIS project},

author = {Adeline Janus and Alina Ghinet and Christophe Waterlot},

year  = {2022},

date = {2022-01-01},

booktitle = {International Conference on Soils, Sediments and water (InterSoil'2022), 

	5-6 October, Bruxelles (Belgium)},

note = {ACTI},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Schnackenberg2022,

title = {Effects of amendments on the mobility, bioaccessibility, and phytoavailability of As, Cd, Pb, and Zn in contaminated urban kitchen garden soils},

author = {Ashley Schnackenberg and Géraldine Bidar and Valérie Bert and Patrice Cannavo and Sébastien Détriché and Francis Douay and René Guenon and Liliane Jean-Soro and Alice Kohli and Thierry Lebeau and Karen Perronnet and Laure Vidal-Beaudet and Christophe Waterlot and Aurélie Pelfrêne},

year  = {2022},

date = {2022-01-01},

booktitle = {11th Conference on Soils of Urban, Industrial, Traffic and Mining Areas (SUITMA), 5-9 September 2022, Berlin (Germany)},

abstract = {Urban gardening is a growing trend that responds to the need for nature in cities, a growing movement towards healthy and sustainable eating and food production, and difficult economic and societal contexts. Both private and community gardens in these areas are often on urban wastelands, along roads, highways, or railways, near industrial sites, and have even sometimes earned the term “guerrilla gardens” for their lack of agronomic regulation. In France, many urban gardens are associated with varying levels of inorganic contaminants hailing from anthropogenic activity or geogenic metal(loid) presence. Moderately metal(loid)- contaminated urban kitchen garden soils can benefit from gentle remediation options including the use of soil amendments, which are able to improve soil function and agronomic quality while decreasing environmental and human health risk. This study first analyses the effects of common doses of a various commercialized soil amendments on the mobility of metalloid(s) in contaminated urban kitchen garden soils. Fourteen different amendments and amendment mixes were tested on three kitchen garden soils with diverse sources of anthropogenic or geogenous contamination and varying physico-chemical characteristics. Amendments from four differing categories were tested, including i) organic amendments like composts, ii) natural fertilizers such as bone meal, crushed horn, and organic poultry manure fertilizer, iii) calcareous amendments including two different limes, and iv) natural siliceous and alumino-silicate amendments, including diatomaceous earth and zeolite. Amendments at varying common doses were evaluated via chemical extractions on their ability to reduce the mobility of metallic elements after multiple weeks of maturation at a constant humidity and temperature. The most pertinent of these amendments were then chosen to be tested in conjunction with a vegetal model grown upon the amended soils in greenhouse conditions. 05.09.– 09.09 2022 Berlin, Germany Changes in physico-chemical soil characteristics, mobility and bioaccessibility of metal(loid)s in the soil, and phytoavailability in the lettuce were then tested. Mobility and bioaccessible metal(loid)s were evaluated with 0.05 M EDTA buffered at a pH of 7, an ammonium nitrate extraction, a 4-phase sequential extraction, and a UBM bioaccessibility test. Phytoavailability was determined using pseudo-total soil and plant metal(loid) contents. The study results show that it is possible to reduce both extractable and phytoavailable metal(oid)s with amendment addition. However, variable effects are seen according to the contaminated soil tested, and differences arise in the presence of a plant model.},

note = {ACTI},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Vuillemin2022,

title = {Combining Phytoremediation and Biorefinery Strategies Assisted by 

	an Ecofriendly Zwitterionic Ionic Liquid: A New Challenge for a Cleaner 

	Bioeconomy},

author = {Marie Vuillemin and Christophe Waterlot and Anthony Verdin and Sylvain Laclef and Christine Cézard and David Lesur and Catherine Sarazin and Dominique Courcot and Caroline Hadad and Eric Husson and Albert Nguyen-van-Nhien},

year  = {2022},

date = {2022-01-01},

journal = {SSRN},

pages = {27pp},

abstract = {This study aims to investigate the ability of an imidazolium biobased Zwitterionic Ionic Liquids (ZILs) in enhancing the phytoextraction of copper from garden and vineyard soils using the model plant ryegrass (Lolium perenne L.). The copper/ZIL molar ratio (1/4) introduced was rationally established based on molecular modeling and on the maximal copper concentration in artificially contaminated soil. Higher accumulation of copper in the shoots was detected for the uncontaminated and copper contaminated ZIL amended soils (18.9 and 23.3 mg.kg-1, respectively) together with a similar ZIL concentration of around 3% w/w detected by LC-MS analyses. These data evidenced a phytoextraction improvement of 38 and 66% compared to non-amended soils. ZIL would be mainly present under Cu(II)-ZIL4 complexes in the shoots. However depending on the soil modalities, the presence of free copper and/or free ZIL led to different chemical compositions in lignin and monomeric sugar contents. In the biorefinery context, performances of enzymatic hydrolysis of shoots were related to the presence of both ZIL and copper under free or complex forms. Ecotoxicity of the different soils indicated that the quantity of copper and ZIL remaining in the soils had no significant toxicity. ZIL amendment in a copper-contaminated soil was demonstrated as being a promising way to promote the valorization of phytoremediation plants.},

note = {ACLO},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Waterlot2022,

title = {Biomass valorization},

author = {Christophe Waterlot},

year  = {2022},

date = {2022-01-01},

urldate = {2022-01-01},

booktitle = {Current challenges of environmental contamination & its impact on  the ecosystems and human health, 4-5 juillet, Lille},

note = {ACTI},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Ofori-Agyeman2022,

title = {Optimization of phytomanagement strategies on soils contaminated with metals (Cd, Pb, Zn) to provide biomass for clean biofuel production – Experience from a pot trial},

author = {Felix Ofori-Agyemang and Christophe Waterlot and Michel Mench and Nadège Oustrière},

year  = {2022},

date = {2022-01-01},

urldate = {2022-01-01},

booktitle = {21st International Conference- Exhibition on Soils, Sediments and Water (INTERSOL), 21-23 June, Lyon},

note = {ACTI},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Ofori-Agyeman2022a,

title = {Optimizing phytomanagement strategies on a metal (Cd, Pb, Zn, and 	Cu)-contaminated soil to provide biomass for clean biofuel production – progress from pot trial to Cd and Zn mixture},

author = {Felix Ofori-Agyemang and Christophe Waterlot and Michel Mench and Nadège Oustrière},

year  = {2022},

date = {2022-01-01},

urldate = {2022-01-01},

booktitle = {8th European Bioremediation Conference, 12-17 June, Chania (Greece)},

pages = {224},

note = {ACTI},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{schnackenberg:hal-03583334,

title = {Effects of Inorganic and Organic Amendments on the Predicted Bioavailability of As, Cd, Pb and Zn in Kitchen Garden Soils},

author = {Ashley Schnackenberg and Géraldine Bidar and Valérie Bert and Patrice Cannavo and Sébastien Détriché and Francis Douay and René Guenon and Liliane Jean-Soro and Alice Kohli and Thierry Lebeau and Karen Perronnet and Laure Vidal-Beaudet and Christophe Waterlot and Aurélie Pelfrêne},

url = {https://hal.archives-ouvertes.fr/hal-03583334},

doi = {10.21926/aeer.2201004},

year  = {2022},

date = {2022-02-01},

urldate = {2022-02-01},

journal = {Advances in Environmental and Engineering Research},

volume = {3},

pages = {1 - 1},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Guerin2022,

title = {Removal of heavy metals from contaminated water using industrial 	wastes containing calcium and magnesium},

author = {Théo Guérin and Nadège Oustrière and David Bulteel and Damien Betrancourt and Alina Ghinet and Sandhya Malladi and Justice-G. Kaleo-Bioh and Amaury Blanc-Brude and Abraham Pappoe and Christophe Waterlot},

year  = {2022},

date = {2022-01-01},

urldate = {2022-01-01},

journal = {Journal of Cleaner Production},

volume = {337},

pages = {130472},

abstract = {Two industrial wastes (IW1 and IW2) were investigated as potential sorbents to retain cadmium and lead from contaminated water. The sorption experiments have been conducted through lab-engineered cartridge filtration system in order to get as close as possible to industrial processes. The effectiveness of IW1 and IW2 were compared to that of activated charcoal Norit® (AC), the best-known matrix for its excellent retention capacity. The sorption isotherms of metals on the three solid sorbents (IW1, IW2 and AC) were built, and then mathematically modelled. Free Gibbs energy (ΔG°) of the sorption processes as well as the equilibrium parameter (RL) have been calculated for each pollutant-sorbent couple. The study revealed that: i) for cadmium sorption, IW2 was much more effective than IW1 and especially than AC (18-fold higher in term of maximal sorption capacity); ii) if IW2 was slightly less effective than AC to retain lead, it can be still considered as an interesting sorbent due to its low cost; iii) the sorption of cadmium was as spontaneous on AC as on IW1, and almost half as much on IW2; iv) regarding lead retention, the sorption on the three sorbents was spontaneous; (v) the retention of cadmium and lead was mainly explained by precipitation since otavite, cerussite and hydrocerussite were identified and characterized by X-ray diffraction of used sorbents. The study showed that the industrial wastes studied stood out as new efficient materials with sorption power equal to or greater than the reference material AC. The prospect of a new generation of industrial wastes with lasting efficiency as contaminated water depollution agents comes at a key moment in the search for new perspectives in the circular economy.},

note = {ACL},

keywords = {ER2, ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Hechelski2021,

title = {Biomass of ryegrass from field experiments: toward a cost-effective and efficient biosourced catalyst for the synthesis of Moclobemide},

author = {Marie Hechelski and Christophe Waterlot and Pierrick Dufrénoy and Brice Louvel and Adam Daïch and Alina Ghinet},

year  = {2021},

date = {2021-01-01},

journal = {Green Chemistry Letters and Reviews},

volume = {14},

number = {1},

pages = {13-20},

abstract = {Lolium perenne L., a common plant perennial ryegrass from Europe, 

	northern Africa and Asia, was selected to produce biomass under field 

	conditions. The biomass of this nonfood crop was from a highly contaminated 

	agricultural soil by potentially toxic metals (Cd, Pb and Zn) with 

	the aim of maintaining the agricultural vocation of these soils. 

	In this perspective and in view of the metal concentration (Cd, Pb, 

	Zn, macro- and micro-nutrients), the biomass of ryegrass was considered 

	as a bio ’ore’ resource and was used as the starting material for 

	the preparation of contemporary biosourced catalysts. The heterogeneous 

	catalyst from the current work was successfully used in the synthesis 

	of Moclobemide, used to treat anxiety and major depressive episodes. 

	Through this original approach, Moclobemide was synthesized in one 

	step, in 80% yield under solvent-free conditions.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{cornu2021advantages,

title = {Advantages and limits to copper phytoextraction in vineyards},

author = {Jean-Yves Cornu and Christophe Waterlot and Thierry Lebeau},

doi = {https://doi.org/10.1007/s11356-021-13450-3},

year  = {2021},

date = {2021-01-01},

journal = {Environmental Science and Pollution Research},

pages = {1--10},

publisher = {Springer},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{su13010034,

title = {Toward a New Way for the Valorization of Miscanthus Biomass Produced on Metal-Contaminated Soils Part 2: Miscanthus-Based Biosourced Catalyst: Design, Preparation, and Catalytic Efficiency in the Synthesis of Moclobemide},

author = {Théo Guérin and Alina Ghinet and Christophe Waterlot},

url = {https://www.mdpi.com/2071-1050/13/1/34},

doi = {10.3390/su13010034},

issn = {2071-1050},

year  = {2021},

date = {2021-01-01},

journal = {Sustainability},

volume = {13},

number = {1},

abstract = {The conception of two biosourced catalysts (biocatalysts) using stems of miscanthus from the first part of this study are described herein. The temperature and the process used to extract metals from plant as mixture of Lewis acids were investigated in detail and proved to be essential in the design of the biosourced catalysts and their catalytic efficiency. One part of the crude mixture of Lewis acids extracted from the aerial parts of miscanthus plants was used without further treatment as a homogeneous biocatalyst (M1), and the other part was supported on montmorillonite K10 to provide a heterogeneous biocatalyst (MM1). M1 and MM1 were next tested in the synthesis of moclobemide (main ingredient of a drug used to treat depression) and led to excellent yield. Additional comparative experiments with different commercial metallic salts (NaCl, KCl, CaCl2, MgCl2, CuCl2, ZnCl2, FeCl2, FeCl3, MnCl2, and AlCl3) and their mixtures were carried out and underlined the importance of the multimetallic synergy on catalytic activity. Finally, a comparison of this new synthetic method assisted by the biosourced catalyst with the previously described procedures to access moclobemide was realized by calculating their green chemistry metrics. This study revealed that the use of the biosourced catalyst led to one of the greenest synthetic methods described today to produce moclobemide.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{doi:10.1080/17518253.2020.1856943,

title = {Biomass of ryegrass from field experiments: toward a cost-effective and efficient biosourced catalyst for the synthesis of Moclobemide},

author = {Marie Hechelski and Christophe Waterlot and Pierrick Dufrénoy and Brice Louvel and Adam Daïch and Alina Ghinet},

url = {https://doi.org/10.1080/17518253.2020.1856943},

doi = {10.1080/17518253.2020.1856943},

year  = {2021},

date = {2021-01-01},

urldate = {2021-01-01},

journal = {Green Chemistry Letters and Reviews},

volume = {14},

number = {1},

pages = {15-22},

publisher = {Taylor & Francis},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{NSANGANWIMANA2021112125,

title = {Potentials of Miscanthus x giganteus for phytostabilization of trace element-contaminated soils: Ex situ experiment},

author = {Florien Nsanganwimana and Karim Suhail Al Souki and Christophe Waterlot and Francis Douay and Aurélie Pelfrêne and Andrea Ridošková and Brice Louvel and Bertrand Pourrut},

url = {https://www.sciencedirect.com/science/article/pii/S0147651321002360},

doi = {https://doi.org/10.1016/j.ecoenv.2021.112125},

issn = {0147-6513},

year  = {2021},

date = {2021-01-01},

urldate = {2021-01-01},

journal = {Ecotoxicology and Environmental Safety},

volume = {214},

pages = {112125},

abstract = {Phytomanagement is proposed as a cost-effective and environmentally-friendly suggestion for sustainable use of large metal-contaminated areas. In the current work, the energy crop miscanthus (Miscanthus × giganteus) was grown in ex situ conditions on agricultural soils presenting a Cd, Pb and Zn contamination gradient. After 93 days of culture, shoot and root growth parameters were measured. Soils and plants were sampled as well to study the TE accumulation in miscanthus and the effects of this plant on TE mobility in soils. Results demonstrated that miscanthus growth depended more on the soils silt content rather than TE-contamination level. Moreover, soil organic carbon at T93 increased in the soils after miscanthus cultivation by 25.5–45.3%, whereas CaCl2-extractible TEs decreased due to complex rhizosphere processes driving plant mineral uptake, and organic carbon inputs into the rhizosphere. In the contaminated soils, miscanthus accumulated Cd, Pb and Zn mainly in roots (BCF in roots: Cd " Zn > Pb), while strongly reducing the transfer of these elements from soil to all organs and from roots to rhizomes, stems and leaves (average TFs: 0.01–0.06, 0.11–1.15 and 0.09–0.79 corresponding to Cd, Pb and Zn respectively). Therefore, miscanthus could be considered a TE-excluder, hence a potential candidate crop for coupling phytostabilization and biomass production on the studied Metaleurop TE-contaminated soils.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@inbook{10.1007/978-3-030-51210-1_92,

title = {Histopathological Changes in the Hepatopancreas of Porcellio Laevis (Crustacea, Isopoda) After Exposure to Cd and Zn Mixture},

author = {Chedliya Ghemari and Raja Jelassi and Hajer Khemaissia and Christophe Waterlot and Maryline Raimond and Catherine Souty-Grosset and Francis Douay and Karima Nasri-Ammar},

editor = {Mohamed Ksibi and Achraf Ghorbal and Sudip Chakraborty and Helder I. Chaminé and Maurizio Barbieri and Giulia Guerriero and Olfa Hentati and Abdelazim Negm and Anthony Lehmann and Jörg Römbke and Armando Costa Duarte and Elena Xoplaki and Nabil Khélifi and Gilles Colinet and Jo ao Miguel Dias and Imed Gargouri and Eric D. Van Hullebusch and Benigno Sánchez Cabrero and Settimio Ferlisi and Chedly Tizaoui and Amjad Kallel and Sami Rtimi and Sandeep Panda and Philippe Michaud and Jaya Narayana Sahu and Mongi Seffen and Vincenzo Naddeo},

doi = {https://doi.org/10.1007/978-3-030-51210-1_92},

isbn = {978-3-030-51210-1},

year  = {2021},

date = {2021-01-01},

urldate = {2021-01-01},

booktitle = {Recent Advances in Environmental Science from the Euro-Mediterranean and Surrounding Regions (2nd Edition)},

pages = {587--592},

publisher = {Springer International Publishing},

address = {Cham},

abstract = {The ultrastructural changes on the hepatopancreas of Porcellio laevis individuals were investigated after their exposure to a mixture of cadmium (Cd) and zinc (Zn) during four weeks. At the end of the experiment, trace element (TE) concentrations were determined using atomic absorption spectrometry (AAS). The growth of individuals was calculated and compared among the treatments. From the first week until the end of the experiment, a weight gain was observed in P. laevis individuals. Additionally, the TE concentrations in the hepatopancreas and in the rest of the body were considerable and seem to be dose dependent. Moreover, our results confirmed the role of the hepatopancreas as the main storage organ. Thus, the conducted transmission electron microscopy highlighted remarkable alterations on the hepatopancreas cells resulting from the Cd and Zn bioaccumulation. The displayed alterations are principally the disorganization of the S- and B-cells leading to a destruction of the microvilli border, an increase of the lipid droplets and mitochondria as well as the appearance of trace elements in the form of B-type granules. Based on the obtained results herein, we can conclude that Porcellio laevis might be a good candidate in the prevention of TE pollution in the terrestrial ecosystems.},

note = {OS},

keywords = {ER4},

pubstate = {published},

tppubtype = {inbook}

}

@article{guerin:hal-03772849,

title = {Ecocatalysed Hurtley reaction: Synthesis of urolithin derivatives as new potential RAGE antagonists with anti-ageing properties},

author = {Théo Guérin and Christophe Waterlot and Emmanuelle Lipka and Philippe Gervois and David Bulteel and Damien Betrancourt and Carla Moignard and Adrian Sorin Nica and Christophe Furman and Alina Ghinet},

url = {https://hal.science/hal-03772849},

doi = {10.1016/j.scp.2021.100518},

year  = {2021},

date = {2021-10-01},

urldate = {2021-10-01},

journal = {Sustainable Chemistry and Pharmacy},

volume = {23},

pages = {100518},

publisher = {ELSEVIER},

note = {ACL},

keywords = {ER2, ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Ghemari2020,

title = {Physiological and histopathological responses of Porcellio laevis (Isopoda, Crustacea) as indicators of metal trace element contamination},

author = {Chedliya Ghemari and Raja Jelassi and Hajer Khemaissia and Christophe Waterlot and Maryline Raimond and Catherine Souty-Grosset and Francis Douay and Karima Nasri-Ammar},

year  = {2020},

date = {2020-01-01},

journal = {Microscopy Research and Technique},

volume = {83},

number = {4},

pages = {402-409},

abstract = {Abstract This study was designed to assess the impact of the mixture 

	of cadmium (Cd) and zinc (Zn) on the bioaccumulation and the ultrastructural 

	changes in the hepatopancreas of Porcellio laevis (Latreille, 1804) 

	after 4?weeks of exposure to contaminated Quercus leaves under laboratory 

	conditions. For each metal, four concentrations were used with four 

	replicates for each concentration. Metal concentrations in the hepatopancreas 

	and the rest of the body were determined using atomic absorption 

	spectrometry. From the first week until the end of the experiment, 

	a weight gain in P. laevis was observed particularly between the 

	first and the end of exposure from 93.3?±?18.22?mg fw to 105.22?±?16.16?mg 

	fw and from 106.4?±?22.67?mg fw to 125.9?±?23.9 mg fw for Mix1 

	and Mix4, respectively. Additionally, the determined metal trace 

	elements (MTE) concentrations in the hepatopancreas were considerably 

	higher compared to those in the rest of the body and seem to be dose-dependent. 

	Using transmission electron microscopy (TEM), some alterations were 

	highlighted in the hepatopancreas. The main observed alterations 

	were (a) the destruction of the microvilli border in a considerable 

	portion of cells, (b) the increase of the lipid droplets with different 

	shapes and sizes, (c) the increase in the number of the mitochondria, 

	and (d) the appearance of TE in the form of B-type granules. The 

	obtained results confirmed the ability of P. laevis to deal with 

	high amounts of MTE, suggesting its possible use in future soil's 

	biomonitoring programs.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Janus2020,

title = {Ex situ evaluation of the effects of biochars on environmental and toxicological availabilities of metals and polycyclic aromatic hydrocarbons},

author = {Adeline Janus and Christophe Waterlot and Francis Douay and Aurélie Pelfrêne},

year  = {2020},

date = {2020-01-01},

journal = {Environmental Science and Pollution Research},

volume = {27},

pages = {1852–1869},

abstract = {The present study experimented five biochars, one made from wood (400 

	°C, 12 h) and four made from miscanthus cultivated on contaminated 

	soils (temperature 400/600 °C, duration 45/90 min). They were used 

	as amendments at a 2% application rate on soil, cultivated or not 

	cultivated with ryegrass, contaminated with (i) metals (Cd, Pb, and 

	Zn), (ii) eight polycyclic aromatic hydrocarbons (PAHs), and (iii) 

	a mix of metals and PAHs. The objectives were (i) to compare the 

	effectiveness of the five biochars on soil parameters and pollutant 

	availability and (ii) to determine the influence of soil multicontamination 

	and ryegrass cultivation on biochar effectiveness. The results showed 

	that biochar application did not necessarily lead to lower pollutant 

	extractability and metal bioaccessibility. However, differences were 

	highlighted between the biochars. The miscanthus biochars produced 

	at 600 °C (BM600) showed higher effectiveness at decreasing metal 

	extractability than the miscanthus biochars produced at 400 °C (BM400) 

	due to its better sorption characteristics. In addition, ryegrass 

	cultivation did not impact pollutant availability but modified metal 

	bioaccessibility, especially for the soil amended with the BM600 

	and the woody biochar. Moreover, the presence of PAHs also negatively 

	impacted the metal bioaccessibility in the soil amended with the 

	BM600, and, on the contrary, positively impacted it in the soil amended 

	with the BM400. Complementary studies are therefore necessary to 

	understand the mechanisms involved, particularly in a context where 

	soils requiring remediation operations are often multicontaminated 

	and vegetated.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Bidar2020,

title = {Urban kitchen gardens: Effect of the soil contamination and parameters on the trace element accumulation in vegetables – A review},

author = {Géraldine Bidar and Aurélie Pelfrêne and Christophe Schwartz and Christophe Waterlot and Karin Sahmer and Franck Marot and Francis Douay},

year  = {2020},

date = {2020-01-01},

journal = {Science of the Total Environment},

volume = {738},

pages = {139569},

abstract = {Trace element contaminants in kitchen garden soils can contribute 

	to human exposure through the consumption of homegrown vegetables. 

	In urban areas, these soils can be contaminated to various degrees 

	by trace element (TE). They are characterized by a great variability 

	in their physicochemical parameters due to the high anthropization 

	level, the wide variety and combination of disturbance sources, aswell 

	as the diversity of cultivation practices and the large range of 

	contamination levels. Pollutants can be taken up by vegetables cultivated 

	in these soils and be concentrated in their edible parts. In this 

	review, the behavior of vegetables cultivated in contaminated kitchen 

	gardens is assessed through six examples of the most widely cultivated 

	vegetables (lettuce, tomato, bean, carrot, radish, potato). The role 

	of soil parameters that could influence the uptake of As, Cd, Cr, 

	Ni, Pb, and Zn by these vegetables is also discussed.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Bouchard2020,

title = {Etude des performance agroenvironnementales de différents outils d’aide à la decision (OAD) en fertilisation azotée et développement d’une méthode de pilotage dynamique},

author = {Marie-Astrid Bouchard and Herinaina-A.-L. Andriamandroso and Sitraka Andrianarisoa and Ali Siah and Christophe Waterlot and A Carlier and Sébastien Dandrifosse and C Guille and Bertrand Vandoorne},

year  = {2020},

date = {2020-01-01},

booktitle = {Phloème, 29-30 janvier 2020, Cité des Sciences & de l’Industrie, Paris},

note = {AFF},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Dufrenoy2020,

title = {New efficient eco-friendly supported catalysts for the synthesis of amides with antioxidant and anti-inflammatory properties},

author = {Pierrick Dufrénoy and Rogatien Charlet and Marie Hechelski and Adam Daïch and Christophe Waterlot and Samir Jawhara and Alina Ghinet},

year  = {2020},

date = {2020-01-01},

journal = {ChemMedChem},

volume = {15},

pages = {459-467},

abstract = {A new environmentally friendly approach for the synthesis of idrocilamide 

	(1), a marketed myorelaxant and anti-inflammatory agent, is reported 

	herein. The synthetic strategy involves a solvent-free aminolysis 

	reaction catalyzed by zinc-containing species (ZnCl2, montmorillonite 

	K10 (MK10) impregnated with ZnCl2 or eco-catalysts). The latter have 

	been prepared from the aerial parts of Lolium perenne L. plants grown 

	on contaminated soils from northern France without and with thermal 

	activation at 120 °C and supported on MK10 (Ecocat1 and Ecocat2, 

	respectively). The best aminolysis catalysts in the current study 

	(ZnCl2 and Ecocat2) were selected for additional aminolyses. Compared 

	to ZnCl2, Ecocat2 had the advantage of being reusable over five test 

	runs and constituted a sustainable catalyst allowing a green route 

	to idrocilamide. Synthesized derivatives 1–4, 6 and 9 were first 

	evaluated for their effect on reactive oxygen species (ROS) generation 

	from macrophages and displayed antioxidant properties by preventing 

	ROS production. Next, the analysis of the effect of molecules 1–4, 

	6 and 9 on macrophage migration between epithelial cells to human 

	opportunistic fungus Candida albicans indicated that molecules 2–4, 

	6 and 9 exert anti-inflammatory properties via reducing macrophage 

	migration while the parent idrocilamide (1) did not show any significant 

	effect. This work opens the way for the discovery of new analogues 

	of idrocilamide with improved properties.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Ghemari2020b,

title = {Bioaccumulation of heavy metals in the terrestrial isopod Porcellionides pruinosus in the vicinity of Gabes-Ghannouch industrial complex},

author = {Chedliya Ghemari and Christophe Waterlot and Anas Ayari and Francis Douay and Karima Nasri-Ammar},

year  = {2020},

date = {2020-01-01},

journal = {Human and Ecological Risk Assessment: An International Journal},

volume = {26},

number = {5},

pages = {1270-1284},

abstract = {The current study reports the effects of heavy metals issued from 

	the emissions of phosphate and their bioaccumulation in the soils, 

	leaf litter, and the terrestrial isopod Porcellionides pruinosus 

	in the vicinity of Gabes-Ghannouch industrial complex. Cd, Pb, Zn, 

	and Cu concentrations were measured in soils, leaf litter, and P. 

	pruinosus individuals, collected from four stations located at different 

	distances from the factory. Results showed that heavy metal concentrations 

	in soils varied from one site to another and did not reflect those 

	measured in individuals. In contrast, a positive correlation was 

	found between the isopods’ body length and the distance from the 

	pollution source, revealing the isopod sensitivity to the soil metal 

	concentrations. The bioaccumulation factor was calculated using soil 

	and leaf litter as a solid support with the aim of ordering the metal 

	accumulation in P. pruinosus for each site. Overall, P. pruinosus 

	could be defined as a macroconcentrator of Cd, Zn, and Cu (BAF>2) 

	and as a deconcentrator of Pb (BAF<1).},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Pelfrene2020,

title = {Evaluation of single-extraction methods to estimate the oral bioaccessibility of metal(loid)s in soils},

author = {Aurélie Pelfrêne and Karin Sahmer and Christophe Waterlot and Philippe Glorennec and Francis Douay and Barbara Le-Bot},

year  = {2020},

date = {2020-01-01},

journal = {Science of the Total Environment},

volume = {727},

pages = {138553},

abstract = {Incidental ingestion of polluted soil particles exposes the population 

	to toxic metal(loid)s. To refine the methods of exposure and risk 

	assessment, it is relevant to use bioaccessible concentrations of 

	metal(loid)s determined via in vitro digestion methods. However, 

	some validated methods are complex and costly, involving high technical 

	skills and numerous reagents. The objective of the present study 

	was to evaluate the suitability of four simple chemical extractions 

	to mimic the bioaccessible fraction of As, Cd, and Pb in the gastric 

	(G) and gastrointestinal (GI) phases obtained using the validated 

	UBM (unified bioaccessibility method) test. Acetic acid (0.11 M), 

	citric acid (0.11M), EDTA (0.16 M), and hydrochloric acid (HCl, 0.65%)were 

	separately tested in 201 soil sampleswith a wide range of physicochemical 

	parameters and metal(loid)s concentrations. Significant linear relationships 

	were observed with HCl, EDTA, and to a lesser extent with citric 

	acid. For the cheaper HCl method, correlations with the UBM ranged 

	from 0.91 to 0.99 for the G phase and from 0.72 to 0.97 for the GI 

	phase. This test can be used at least as a first-tier screening to 

	assess the oral bioaccessibility of As, Cd, and Pb.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Guerin2020,

title = {Wheat and ryegrass biomass ashes as effective sorbents for metallic and organic pollutants from contaminated water in lab-engineered cartridge filtration system},

author = {Théo Guérin and Alina Ghinet and Marc Hossarte and Christophe Waterlot},

year  = {2020},

date = {2020-01-01},

journal = {Bioresource Technology},

volume = {318},

pages = {124044},

abstract = {Three plant biomasses (miscanthus, ryegrass and wheat) have been considered 

	for the preparation of five different sorbents evaluated for their 

	potential to sorb cadmium and lead and four emergent organic compounds 

	(diclofenac, sulfamethoxazole, 17α-ethynylestradiol and triclosan) 

	from artificially contaminated water. Labcreated cartridges were 

	filled with each sorbent and all experiments were systematically 

	compared to activated charcoal Norit®. Results from activated charcoal, 

	wheat straw and acidified wheat straw were supported by the Langmuir 

	and Freundlich models. Wheat straw ashes were an excellent metal 

	extractor that exceeded the potential of well-known activated charcoal. 

	Acidified sorbents (wheat and ryegrass) were very effective in eliminating 

	the selected emerging organic contaminants displaying equipotent 

	or superior activity compared to activated charcoal. These results 

	open the way for further in natura studies by proposing new biosource 

	materials as new effective tools in the fight against water pollution.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Hechelski2020,

title = {Toward a new way for the valorization of miscanthus biomass produced on metal-contaminated soils Part 1: Mesocosm and field experiments},

author = {Marie Hechelski and Brice Louvel and Pierrick Dufrénoy and Alina Ghinet and Christophe Waterlot},

year  = {2020},

date = {2020-01-01},

journal = {Sustainability},

volume = {12},

pages = {9370},

abstract = {The effects of P-fertilizers (mono- and di-calcium phosphates) on 

	the bioavailability of metals and nutrients in leaves and stems of 

	Miscanthus × giganteus were studied in mesocosm and field experiments 

	in order to propose a new way for the valorization of miscanthus 

	biomass. The concentration of potentially toxic elements was generally 

	higher in stems than in leaves. Although P-fertilizers were added 

	to contaminated soils under sustainable conditions (from 0.022% to 

	0.026% w/w), the average of leaf and stem biomass generally increased 

	in the presence of P-fertilizers due to the changes in the speciation 

	of phosphorus. Leaves of the investigated miscanthus may be of great 

	interest as a catalyst in organic chemistry, since the Ca concentration 

	was up to 9000 mg kg−1 DW. Stems represent a potential biomass that 

	can be used as renewable resource of Lewis acids, currently used 

	in organic syntheses (the sum of Zn, Cu, Mn, Fe, Mg, Si and Al was 

	near 1000 mg kg−1 DW). The percentage of Cd and Pb in leaves and 

	stems of miscanthus did not significantly change with P-fertilizers. 

	Depending on the mesocosm and field experiments, it ranged from 0.004% 

	to 0.016% and from 0.009% and 0.034% for Cd in leaves and stems, 

	respectively, and from 0.004% to 0.015% and from 0.009% and 0.033% 

	for Pb in leaves and stems, respectively.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@book{Pelfrene2020c,

title = {Spécificités des potagers en contexte (péri)urbain au lourd passé industriel},

author = {Aurelie Pelfrêne and Géraldine Bidar and Sébastien Détriché and Christophe Waterlot and Christelle Pruvot and Francis Douay},

year  = {2020},

date = {2020-01-01},

booktitle = {in "Les sols urbains sont-ils cultivables ?" (Editions Quae, Collection Matière à débattres & décider)},

note = {OS},

keywords = {ER4},

pubstate = {published},

tppubtype = {book}

}

@article{ALSOUKI2020110654,

title = {Miscanthus x giganteus culture on soils highly contaminated by metals: Modelling leaf decomposition impact on metal mobility and bioavailability in the soil–plant system},

author = {Al Souki Karim Suhail and Clarisse Liné and Brice Louvel and Christophe Waterlot and Francis Douay and Bertrand Pourrut},

url = {https://www.sciencedirect.com/science/article/pii/S0147651320304930},

doi = {https://doi.org/10.1016/j.ecoenv.2020.110654},

issn = {0147-6513},

year  = {2020},

date = {2020-01-01},

urldate = {2020-01-01},

journal = {Ecotoxicology and Environmental Safety},

volume = {199},

pages = {110654},

abstract = {Miscanthus x giganteus is suggested as a good candidate for phytostabilization of metal-polluted soils. Its late harvest in winter generates large amounts of leaf litter on the soil surface. However, little is known about the mobility and the bioavailability of metals following leaf decomposition and the consequences on the succeeding culture. Ex situ artificial aging for 1, 3, and 6 months was conducted with miscanthus leaf fragments incorporated into three agricultural soils displaying a gradient concentration in Cd (0.6, 3.1 and 7.9 mg kg−1), Pb (32.0, 194.6 and 468.6 mg kg−1), and Zn (48.4, 276.3 and 490.2 mg kg−1) to simulate the leaf litter input over 20 years of miscanthus culture. We investigated the impacts on physicochemical and biological soil parameters, CaCl2-extractable metal, and their subsequent ryegrass shoot concentrations, and hence on ryegrass health. The results showed that the amended soils possessed higher pH along with greater available phosphorous and soil organic carbon values. The respiratory activity and microbial biomass carbon in the amended soils increased mainly after 1 month of aging, and decreased afterwards. Despite the higher Pb- and Zn–CaCl2 extractability in the amended soils, the phytoavailability slightly increased only in the most contaminated soils. Moreover, leaf incorporation did not affect the ryegrass biomass, photosynthetic pigment contents, nor the antioxidative enzyme activities. Conclusively, leaf incorporation induced slight variations in soil physicochemical and biological parameters, as well as metal extractability, but not to an extent that might cause a considerable threat to the subsequent culture. Nevertheless, these results are preliminary data that require confirmation by long-term in-situ experimentations as they reflect the modelization of long-term impact of leaf decomposition on soil-plant system.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{nokey,

title = {Ecocatalyzed synthesis of valuable therapeutic molecules},

author = {Théo Guérin and Christophe Waterlot and Alina Ghinet},

year  = {2020},

date = {2020-01-19},

urldate = {2020-01-19},

address = {St Anton, Austria},

note = {2nd Alpine Winter Conference on Medicinal and Synthetic Chemistry, St Anton, Austria, COM},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Dufrenoy2019,

title = {An innovative and efficient method to synthesize meloxicam in one-step procedure with respect to the green chemistry},

author = {Pierrick Dufrénoy and Alina Ghinet and Marie Hechelski and Adam Daïch and Christophe Waterlot},

year  = {2019},

date = {2019-01-01},

journal = {Journal of the Iranian Chemical Society},

volume = {16},

number = {3},

pages = {501-509},

abstract = {An improved procedure for the synthesis of meloxicam drug (methyl 

	4-hydroxy-2-methyl-2H-1,2-benzothiazol-2-amine-3-carboxylate 1,1-dioxide) 

	was described in one-step using mainly impregnated montmorillonite 

	K10 (MK10) with ZnCl2 as a heterogeneous catalyst. This innovative 

	method was compared to the last described procedure employed in the 

	manufacture of this anti-inflammatory drug by means of some metrics 

	used in a first step of the evaluation process of the environmental 

	impact of a chemical transformation. Apart from the yield, which 

	was 90%, atom economy, waste, environmental factor, reaction mass 

	efficiency and stoichiometric factor were calculated as 91.6%, 8.4%, 

	0, 8.1% and 1%, respectively. Interpretation of these metrics was 

	given and highlighted the fact that the strategy used in the current 

	study may be considered as an environmental-friendly and sustainable 

	method that fits well in the green chemistry concepts.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Ghemari2019,

title = {Histopathological changes in the hepatopancreas of Porcellio laevis (Crustacea, Isopoda) after exposure to Cd and Zn mixture},

author = {Chedliya Ghemari and Raja Jelassi and Hajer Khemaissia and Christophe Waterlot and Maryline Raimond and Catherine Souty-Grosset and Francis Douay and Karima Nasri-Ammar},

year  = {2019},

date = {2019-01-01},

booktitle = {2nd Euro-Mediterranean Conference for Environmental Integration, 10-13 October 2019, Sousse (Tunisia)},

note = {ACTI},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Ghemari2019a,

title = {Effects of Heavy Metals Artificial Contamination on Porcellio laevis (Latreille, 1804) (Crustacea: Isopoda: Oniscidea)},

author = {Chedliya Ghemari and Christophe Waterlot and Anas Ayari and Francis Douay and Karima Nasri-Ammar},

year  = {2019},

date = {2019-01-01},

journal = {Bulletin of Environmental Contamination and Toxicology},

volume = {103},

pages = {416-420},

abstract = {This study aimed at determining the competition of cadmium (Cd), lead 

	(Pb), zinc (Zn) and copper (Cu) on their assimilation, on the food 

	consumption and the growth of terrestrial isopod Porcellio laevis. 

	Individuals were exposed to artificially contaminated litter of Quercus 

	for 4 weeks and were weekly weighed. At the end of the experiment, 

	the concentration of Cd, Pb, Zn and Cu in individuals were measured 

	by atomic absorption spectrometry. Biological parameters such as 

	growth, and bioaccumulation factor (BAF) were calculated and results 

	from the various treatments were compared. Depending on metals, weight 

	loss or gain were recorded for isopods during the four weeks of exposure. 

	A weight loss was measured on individuals exposed to Cd-contaminated 

	litter whereas a weight gain was highlighted for those exposed to 

	the Zn-contaminated litter. BAF values revealed that P. laevis was 

	macroconcentrator of Zn and Cu and deconcentrator of Cd and Pb},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Hechelski2019a,

title = {The potential of ryegrass (Lolium perenne L.) to clean up multi-contaminated soils from labile and phytoavailable potentially toxic elements to contribute into a circular economy},

author = {Marie Hechelski and Brice Louvel and Pierrick Dufrénoy and Alina Ghinet and Christophe Waterlot},

year  = {2019},

date = {2019-01-01},

journal = {Environmental Science and Pollution Research},

volume = {26},

number = {17},

pages = {17489–17498},

abstract = {Aided phytoremediation was studied for 48 weeks with the aim of reducing 

	extractable and phytoavailable toxic elements and producing potential 

	marketable biomass. In this sense, biomass of ryegrass was produced 

	under greenhouse on two contaminated garden soils that have been 

	amended with two successive additions of phosphates. After the first 

	addition of phosphates, seeds of ryegrass were sown and shoots were 

	harvested twice. A second seedling was performed after carefully 

	mixing the roots from the first production (used as compost), soils 

	and phosphates. Forty-eight weeks after starting the experiments, 

	the concentrations of Cd, Pb, Zn, Cu, Fe, and Mn extracted using 

	the rhizosphere-based method were generally lower than those measured 

	before the addition of phosphates and cultivation (except for Pb 

	and Fe in the most contaminated soil). The concentrations of metals 

	in the shoots of ryegrass from the second production were lower than 

	those from the first (except for Al). The best results were obtained 

	with phosphates and were the most relevant in the lowest contaminated 

	soil, demonstrating that the available metal concentrations have 

	to be taken into account in the management of contaminated soils. 

	In view of the concentration of metals defined as carcinogens, mutagens, 

	and reprotoxics (e.g., Cd, Pb) and those capable to be transformed 

	into Lewis acids (e.g., Zn, Fe), the utilization of ryegrass in the 

	revegetation of contaminated soils and in risk management may be 

	a new production of marketable biomass. The development of phytomanagement 

	in combination with this type of biomass coincided with the view 

	that contaminated soils can still represent a valuable resource that 

	should be used sustainably.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Le-Bot2019,

title = {Simplified method for oral bioaccessibility of metal(loid)s in soils and alternative to the Unified Barge Method},

author = {Barbara Le-Bot and Philippe Glorennec and Christophe Waterlot and Francis Douay and Aurélie Pelfrêne},

year  = {2019},

date = {2019-01-01},

booktitle = {International Societies of Exposure Science (ISES) and Indoor Air Quality and Climate (ISIAQ), 18-22 August 2019, Kaunas (Lithuania)},

note = {AFF},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Pelfrene2019a,

title = {Evaluation of four first tier methods to measure metal(loid) bioaccessibility in polluted soils},

author = {Aurélie Pelfrêne and Barbara Le-Bot and Christophe Waterlot and Philippe Glorennec and Karin Sahmer and Francis Douay},

year  = {2019},

date = {2019-01-01},

booktitle = {SETAC Europe, 29th Annual meeting, 26-30 May 2019, Helsinki (Finland)},

note = {ACTI},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Pelfrene2019d,

title = {From environmental data acquisition to assessment of gardeners' exposure: feedback in an urban context highly contaminated with metals},

author = {Aurélie Pelfrêne and Karin Sahmer and Christophe Waterlot and Francis Douay},

year  = {2019},

date = {2019-01-01},

journal = {Environmental Science and Pollution Research},

volume = {26},

number = {20},

pages = {20107-20120},

abstract = {Although growing vegetables in urban gardens has several benefits, 

	some questions in relation with the safety of foods remain when the 

	self-production is carried out on highly contaminated garden soils. 

	To better assess the local population's exposure to Cd and Pb induced 

	by the past activities of a lead smelter, a participatory program 

	was initiated in 115 private kitchen gardens located in northern 

	France to assist gardeners in understanding their soil environment. 

	The challenge included contributing to the database of urban garden 

	soils with the collection of a large number of samples: 1525 crops 

	grouped into 12 types (leaf, fruiting, root, stem and bulbous vegetables, 

	tubers, cabbages, leguminous plants, celeriac, fresh herbs, fruits, 

	and berries), 708 topsoils, and 52 samples of self-produced compost. 

	The main results were as follows: (i) topsoils were strongly contaminated 

	by Cd and Pb compared to regional reference values; (ii) great variability 

	in physicochemical parameters and metal concentrations in topsoils; 

	(iii) the highest concentrations of Cd and Pb for celeriac and fresh 

	herbs and the lowest for fruits and fruiting vegetables; (iv) a high 

	percentage of vegetables that did not comply with the European foodstuff 

	legislation; and (v) most self-produced compost samples were strongly 

	contaminated. This study aimed to raise awareness and generate functional 

	recommendations to reduce human exposure and to provide useful data 

	that could be considered in other environmental contexts.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Waterlot2019,

title = {Benefits of Ryegrass on Multicontaminated Soils Part 1: Effects of Fertilizers on Bioavailability and Accumulation of Metals},

author = {Christophe Waterlot and Marie Hechelski},

year  = {2019},

date = {2019-01-01},

journal = {Sustainability},

volume = {11},

number = {18},

pages = {5093},

abstract = {Effects of three phosphorus fertilizers on the shoot biomass and on 

	the accumulation of alkali, alkaline earth, and transition metals 

	in the shoots and roots of ryegrass were studied with two contaminated 

	garden soils. Phosphates were added in sustainable quantities in 

	order to reduce the environmental availability of carcinogenic metals 

	(e.g., Cd and Pb) and to enhance the bioavailability of alkali and 

	alkaline earth metals as well as micronutrients needed by plants. 

	Addition of Ca(H2PO4)2 was the most convenient way to (i) limit the 

	concentration of Cd and Pb, (ii) keep constant the transfer of macro- 

	and micronutrient from the soil to the ryegrass shoots, (iii) decrease 

	the availability of metals, and (iv) increase the ratio values between 

	potential Lewis acids and Cd or Pb in order to produce biosourced 

	catalysis. For instance, the real phytoavailability was reduced by 

	27%–57% and 64.2%–94.8% for Cd and Pb, respectively. Interestingly, 

	the real phytoavailability of Zn was the highest in the least contaminated 

	soils. Even if soils were highly contaminated, no visual toxicity 

	symptoms were recorded in the growing ryegrasses. This indicates 

	that ryegrass is suitable for the revegetation of contaminated gardens. 

	To promote the sustainable ryegrass production on contaminated soils 

	for production of new organic fragrance and drugs in green processes 

	according to REACH (Registration, Evaluation, Authorisation, and 

	Restriction of Chemicals) regulation, two processes should be recommended: 

	assisted phytostabilization of the elements, and then assisted phytoextraction 

	by using chelators. View Full-Text},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Hechelski2019b,

title = {Effects of calcium phosphates on the (im)mobilization of metals and nutrients, on the biological activity and on the plant health from multi-contaminated urban soils},

author = {Marie Hechelski and Brice Louvel and Pierrick Dufrénoy and Alina Ghinet and Christophe Waterlot},

year  = {2019},

date = {2019-01-01},

journal = {Water, Air, & Soil Pollution},

volume = {230},

number = {8},

pages = {206-223},

abstract = {Two smelters in the North of France emitted potentially toxic metals 

	for more than a century and today, the resulting contamination represents 

	a risk to human health and affects also the biodiversity. To limit 

	health risks and to improve the soil quality, a study using calcium 

	phosphates (monocalcium phosphate, dicalcium phosphate and a mixture 

	of both salts) and Lolium perenne L was conducted. Through this preliminary 

	investigation, we will try to shed some light about (i) the effects 

	of a sustainable amount of calcium phosphates on the agronomic, biological 

	(microbial and fungi communities) and physiological parameters (chlorophyll 

	a and b, antocyanins, carotenoids) as well as the phytoavailability 

	of potentially toxic metals and nutrients in time, and (ii) the potential 

	use of contaminated biomass from ryegrass as a source of new valorisation 

	ways instead of using it as contaminated compost by gardeners. Although 

	slight variations in pH and significant increases of assimilable 

	phosphorus after adding calcium phosphates were registered, the physiology 

	of plants and the biological parameters were statistically unchanged. 

	The germination of the ryegrass seeds was favoured with calcium phosphates 

	regardless the contamination level of the studied soils. No clear 

	effects of calcium phosphates on the microbial and fungi communities 

	were detected. In contrast, results indicated relationships between 

	the physicochemical parameters of soils, their contamination level 

	and the composition of fungal communities. Indeed, for one of the 

	soils studied, calcium could limit the transport of nutrients, causing 

	an increase in fungi to promote again the transfer of nutrients. 

	Surprisingly, the phytoavailability of Pb increased in the most contaminated 

	soil after adding dicalcium phosphate and the mixture of phosphates 

	whereas a slight decrease was highlighted for Cd and Mn. Although 

	minor changes in the phytoavailability of potentially toxic metals 

	were obtained using calcium phosphates, the ability of ryegrass to 

	accumulate Zn and Ca (up to 600 and 20,000 mg kg−1, respectively) 

	make possible to qualify this plant as a bio ‘ore’ resource.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Hechelski2019ab,

title = {A sustainable approach to manage metal-contaminated soils: a preliminary greenhouse study for the possible production of metal-enriched ryegrass biomass for biosourced catalysts},

author = {Marie Hechelski and Brice Louvel and Pierrick Dufrénoy and Alina Ghinet and Christophe Waterlot},

year  = {2019},

date = {2019-01-01},

journal = {Environmental Monitoring and Assessment},

volume = {191},

number = {10},

pages = {626-640},

abstract = {Two kitchen garden soils (A and B) sampled in contaminated areas were 

	amended using phosphates in sustainable quantities in order to reduce 

	the environmental availability of potentially toxic inorganic elements 

	(PTEs) and to favour the availability of alkali, alkali earth and 

	micronutrients. The environmental availability of PTEs was evaluated 

	using a potential plant for revegetation of contaminated soils (ryegrass) 

	and a mixture of low molecular weight organic acids. Despite the 

	highest contamination level of B, the concentration of metals was 

	highest in the ryegrass shoots grown on A for the two harvests. These 

	results correlated well with those obtained using low molecular weight 

	organic acids for Cd, Zn and Cu, whereas this mixture failed to represent 

	the transfer of nutrients due to the presence of biological and physiological 

	mechanisms. The statistical differences between the biomass of ryegrass 

	obtained at the first and the second harvests were attributed to 

	the decrease of available potassium, implicated in the growth and 

	development of plants. Phosphates increased the ratios Zn/Cd, Zn/Pb 

	and Zn/Cu up to 176 ± 48, 38 ± 6 and 80 ± 12, respectively, and made 

	possible the reduction of the concentration of Cd and Pb in the shoots 

	of ryegrass by 22% and 25%, respectively. The concentration of Zn 

	in the shoots of ryegrass from the first and the second harvests 

	grown on soil A were in the range 1050–2000 mg kg−1, making this 

	plant a potential biomass to (i) produce biosourced catalysts for 

	organic chemistry applications in a circular economy concept and 

	(ii) limit human exposure to commercial Lewis acids. A preliminary 

	application was identified.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Pelfrene2019f,

title = {Une méthode simplifiée pour estimer la bioaccessibilité des polluants métalliques dans les sols},

author = {Aurélie Pelfrêne and Barbara Le-Bot and Christophe Waterlot and Philippe Glorennec and Karin Sahmer and Francis Douay},

year  = {2019},

date = {2019-01-01},

booktitle = {4ème Rencontres nationales de la Recherche sur les sites et sols pollués, Le Beffroi de Montrouge, 26-27 novembre 2019, Paris},

note = {ACTN},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Waterlot2019b,

title = {Benefits of Ryegrass on Multicontaminated Soils Part 2: Green process to provide Idrocilamide},

author = {Christophe Waterlot and Pierrick Dufrénoy and Marie Hechelski and Brice Louvel and Adam Daïch and Alina Ghinet},

year  = {2019},

date = {2019-01-01},

journal = {Sustainability},

volume = {11},

number = {23},

pages = {6685-6694},

abstract = {A restoration of highly contaminated garden soil is proposed as a 

	greener alternative to the production of vegetables. Depending on 

	potentially toxic elements and their concentration, ryegrass shoots 

	accumulate these elements in sufficient quantity to be used as a 

	catalyst in organic synthesis. The analysis of Cd, Pb, Zn, Cu, Fe, 

	Mn, and Al in ashes issued from the calcination of ryegrass shoots 

	revealed that the concentration of Zn was highest (>7000 mg kg–1). 

	The ratios between potential Lewis acids (Zn, Cu, Fe, Mn, and Al) 

	to carcinogenic metals (Cd or Pb) were 191 ± 7 for LA/Cd and 235 

	± 13 for LA/Pb, making the shoots of ryegrass suitable for the production 

	of Zn-rich polymetallic biosourced catalysts. This material was used 

	in the synthesis of idrocilamide under free-solvent condition, providing 

	the drug in a good yield (69.9%). Data show that a limitation of 

	waste, a maximization of the material incorporation in the process, 

	a minimization of the steps, and an optimization of the stoichiometric 

	factor are the main innovative factors in the current process in 

	comparison with those previously reported.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Leclercq-Dransar2019,

title = {Distribution of metals and cell wall compounds in leaf parts of three tree species suitable for the phytomanagement of heavy metal–contaminated soils},

author = {Julie Leclercq-Dransart and Sylvain Demuynck and Christophe Waterlot and Géraldine Bidar and Karin Sahmer and Céline Pernin and A Deram and Alain Leprêtre and Francis Douay},

year  = {2019},

date = {2019-01-01},

journal = {Water Air and Soil Pollution},

volume = {230},

number = {237},

pages = {1-16},

abstract = {Phytomanagement used on soils contaminated with metals aims to stabilize 

	them in the soil. It generally uses less demanding tree species such 

	as maple, poplar, and black locust. However, it is important to consider 

	the rate of accumulation of metals in the leaves as well as their 

	location (leaf blade/petiole) together with the contents of cell 

	wall compounds (hemicelluloses, cellulose, lignin). These two aspects 

	are likely not only to have repercussions on the decomposition of 

	litter but also to lead to a possible transfer ofmetal contamination 

	into the food chain via soil decomposers/detritivorous. A successful 

	phytoremediation is therefore the result of compromise: no tree species 

	can meet all these criteria. The results obtained show that poplar 

	is not recommended due to a high accumulation of Cd and Zn. The most 

	appropriate species among our 3 studied species, which seem the most 

	suitable in terms of remediation of metal polluted soils, could be 

	the black locust and the maple which are less susceptible to contribute 

	to the transfer of exogenous metals such as Cd to the trophic chain. 

	However, maple strongly accumulates Pb in the leaf blade and it is 

	susceptible to degrade quickly due to its high hemicelluloses and 

	cellulose contents and low lignin content. The black locust accumulates 

	metals mainly in the petiole (little consumed by detritivorous). 

	However, its exotic character and high lignin content may limit the 

	interest of its use.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Waterlot2018,

title = {Alternative approach to the standard, measurements and testing programme used to establish phosphorus fractionation in soils},

author = {Christophe Waterlot},

year  = {2018},

date = {2018-01-01},

journal = {Analytica Chimica Acta},

volume = {1003},

pages = {26-33},

abstract = {The fractionation of phosphorus in 9 soils was established according	to the standards, measurements and testing (SMT) programme. Five fractions were so defined and phosphorus was analysed by spectrophotometry. In parallel, the phosphorus extracted in each fraction was determined using graphite furnace atomic absorption spectrometry (GFAAS) after	validation of this technique using certified reference materials. Phosphorus in soils was named total phosphorus, inorganic and organic phosphorus, apatite and non-apatite inorganic phosphorus, depending on extractants. The use of both analytical techniques revealed differences between the concentration of phosphorus in soluble extracts and highlighted the fact that spectrophotometry was a selective analytical technique. In view of the resourceand time-consuming of the SMT procedure and the results obtained in the present study, an alternative method was proposed to estimate the fractionation of phosphorus in soil in order to precise the potential effects of phosphorus on plant nutrition when plant biomass is produced as part of metal-contaminated soil management. (C) 2017 Elsevier B.V. All rights reserved.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}