2022Journal Article ER4 Auteurs : Leclercq-Dransart, Julie; Demuynck, Sylvain; Grumiaux, Fabien; Leprêtre, Alain; Lemière, Sébastien; Louvel, Brice; Pernin, Céline; Douay, Francis In: Ecological Engineering, vol. 182, pp. 106694, 2022, (ACL). @article{leclercqdransart:hal-03767130, |
2020Journal Article ER4 Auteurs : Leclercq-Dransart, Julie; Demuynck, Sylvain; Douay, Francis; Grumiaux, Fabien; Pernin, Céline; Leprêtre, Alain Comparison of the interest of four types of organic mulches to reclaim degraded areas: a field study based on their relative attractiveness for soil macrofauna In: Ecological Engineering, vol. 158, pp. 106066, 2020, (ACL). @article{Leclercq-Dransart2020, The study focused on the attractiveness of mulches (consisting of the addition of a layer material to the soil surface) and their efficiency to promote recolonization by pedofauna of isolated and degraded areas. The experimental study was set up in the former coal mining area of Northern France. This area is particularly disturbed and fragmented by land-use change, urbanization, industry, intensive agriculture and metal pollution. To examine the movements of pedofauna, four kinds of mulches were applied: (i) Ramial Chipped Wood (RCW), (ii) chopped Miscanthus, (iii) dead leaves and (iv) wheat straw as well as plastic sheeting, a material often used in green spaces. A negative control consisted in soil without mulch addition. The experimental set-up consisted of a set of 18 bands of mulch (6 m long x 0.5 m width x 8 cm thick, i.e. a volume of 0.04 m3 per band) installed on three adjacent plots on a surface area which stretch on 4400 m2: (i) plot F1, a mixed plantation, (ii) plot M consisted of three rows of maples (both considered to be the reservoir of biodiversity) and (iii) plot BF, an ash plantation (with lower biodiversity). Faunistic communities of soil present within the mulches were monitoring using pitfall traps for four months (April to July), at a rate of eight surveys for each of the 90 traps installed, i.e. 720 samples. Different life traits and indices of diversity were studied, more specifically on carabid beetles. The main objective of the study was first to check whether mulches could or not reconnect different planted plots in a peri-urban landscape highly modified by human activities. The second aim was to find which type of mulch had the best efficiency in the recovery of the connectivity between plots. The last questions were which type of zoological groups could be favoured by the use of mulches and which benefits for ecosystem functioning. The main hypotheses of this study were: (i) Organic mulches would act as dispersal corridors and refuge zones to the soil organisms; (ii) organic mulches would be attractive to many organisms with various ecological needs. This study highlighted the potential benefits of using the mulching technique to support ecological connectivity in disturbed environment since this method highlighted the presence of individuals with different diets (saprophagous, predators, phytophagous and so forth), small and large size species which do not have the same dispersal capacities as well as species that are more dependent on forest environments and others that depend on more open environments. The study concluded that organic mulches are more favourable to pedofauna than soil without mulch or plastic sheeting. With regard to organic mulches, no difference was noted, either in terms of cumulative abundance or monthly monitoring. However, for carabids, it should be noted that dead leaves and wheat straw have higher specific richness than Miscanthus and RCW. |
2019Journal Article ER4 Auteurs : Leclercq-Dransart, Julie; Demuynck, Sylvain; Bidar, Géraldine; Douay, Francis; Grumiaux, Fabien; Louvel, Brice; Pernin, Céline; Leprêtre, Alain Does adding fly ash to metal-contaminated soils play a role in soil functionality regarding metal availability, litter quality, microbial activity and the community structure of Diptera larvae? In: Applied Soil Ecology, vol. 138, pp. 99-111, 2019, (ACL). @article{Leclercq-Dransart2019, This study aimed at evaluating the impact of the use of fly ash-aided phytostabilisation as a method to manage highly metal-contaminated soils on soil functionality taking into account the soil and the litter qualities, the Diptera larvae communities and the microbial activity. To reach this goal, 70 parameters were studied. The main results showed that the two studied fly ashes Soproline® and Sodeline® had a positive influence on soil characteristics especially the availability of metals, nearly 14 years after amendment. Regarding biological parameters, no positive or negative effects were found by adding fly ashes to the soil concerning soil basal respiration and urease activity. The fungal activity was even enhanced. In the same way, the fly ashes had no negative effect on the Diptera larvae community and a greater richness of larvae was even found in amended soils. Thus, this study confirmed that aided-phytostabilisation using these fly ashes should be an advantageous technique to manage metal-polluted soils and to recycle industrial wastes. |
2019Journal Article ER4 Auteurs : Leclercq-Dransart, Julie; Pernin, Céline; Demuynck, Sylvain; Grumiaux, Fabien; Lemière, Sébastien; Leprêtre, Alain Isopod physiological and behavioral responses to drier conditions: An experiment with four species in the context of global warming In: European Journal of Soil Biology, vol. 90, pp. 22-30, 2019, (ACL). @article{Leclercq-Dransart2019b, In the context of global warming, an increase in soil drought is suspected by the IPCC predictions and litter breakdown activity could be affected. An experiment was conducted using microcosms (controlled conditions) and woodlice which are recognized as shredders of litter and bioindicators of global warming. The impact of relative air humidity (90 and 50% RH) on litter breakdown by woodlice was studied through the monitoring of one behavioral (distribution of individuals on microcosms), one morphological (Absolute Growth Rate) and four physiological traits (Survival rate, Relative Consumption Rate, Relative Growth Rate, Feeding rate). Four species of isopods known to have different microclimatic sensitivities (Armadillidium vulgare, Porcellio scaber, Oniscus asellus, Philoscia muscorum) were used simultaneously. Sensitivities of males and females have also been tested. Main results showed that the seven studied parameters under the two relative humidity conditions were not affected in the same way according to the species nor genders. A. vulgare is the most affected species followed by O. asellus. The least affected species are P. scaber and P. muscorum. Their morphological and physiological differences allow to the most evolved species to be more resistant to drier conditions but in return they are no longer active, which can affect soil functionality. With global warming, it could be envisaged that woodlice spend less time foraging and more time seeking refuge to reduce the risk of mortality from desiccation stress. Modifying the feeding behavior of terrestrial isopods could slow down litter degradation with consequences on the process of organic matter recycling. |
2019Journal Article ER4 Auteurs : Marchand, Guillaume; Demuynck, Sylvain; Slaby, Sylvain; Lescuyer, Arlette; Lemière, Sébastien; Marin, Matthieu Adverse effects of fly ashes used as immobilizing agents for highly metal-contaminated soils on Xenopus laevis oocytes survival and maturation-a study performed in the north of France with field soil extracts In: Environmental Science and Pollution Research, vol. 27, pp. 3706–3714, 2019, (ACL). @article{Marchand2019, Amphibians are now recognized as the most endangered group. One of this decline causes is the degradation of their habitat through direct contamination of water, soil leaching, or runoff from surrounding contaminated soils and environments. In the North of France, the extensive industrial activities resulted in massive soil contamination by metal compounds. Mineral amendments were added to soils to decrease trace metal mobility. Because of the large areas to be treated, the use of inexpensive industrial by-products was favored. Two types of fly ashes were both tested in an experimental site with the plantation of trees in 2000. Aim of the present work was to investigate the effects of extracts from metal-contaminated soils treated or not for 10 years with fly ashes on Xenopus laevis oocyte using cell biology approaches. Indeed, our previous studies have shown that the Xenopus oocyte is a relevant model to study the metal ion toxicity. Survival and maturation of oocyte exposed to the soil extracts were evaluated by phenotypic approaches and electrophysiological recordings. An extract derived from a metal-contaminated soil treated for 10 years with sulfo-calcic ashes induced the largest effects. Membrane integrity appeared affected and ion fluxes in exposed oocytes were changed. Thus, it appeared that extracted elements from certain mineral amendments used to prevent the mobility of metals in the case of highly metal-contaminated soils could have a negative impact on X. laevis oocytes. |
2019Journal Article ER4 Auteurs : Leclercq-Dransart, Julie; Demuynck, Sylvain; Waterlot, Christophe; Bidar, Géraldine; Sahmer, Karin; Pernin, Céline; Deram, A; Leprêtre, Alain; Douay, Francis Distribution of metals and cell wall compounds in leaf parts of three tree species suitable for the phytomanagement of heavy metal–contaminated soils In: Water Air and Soil Pollution, vol. 230, no. 237, pp. 1-16, 2019, (ACL). @article{Leclercq-Dransar2019, Phytomanagement used on soils contaminated with metals aims to stabilize them in the soil. It generally uses less demanding tree species such as maple, poplar, and black locust. However, it is important to consider the rate of accumulation of metals in the leaves as well as their location (leaf blade/petiole) together with the contents of cell wall compounds (hemicelluloses, cellulose, lignin). These two aspects are likely not only to have repercussions on the decomposition of litter but also to lead to a possible transfer ofmetal contamination into the food chain via soil decomposers/detritivorous. A successful phytoremediation is therefore the result of compromise: no tree species can meet all these criteria. The results obtained show that poplar is not recommended due to a high accumulation of Cd and Zn. The most appropriate species among our 3 studied species, which seem the most suitable in terms of remediation of metal polluted soils, could be the black locust and the maple which are less susceptible to contribute to the transfer of exogenous metals such as Cd to the trophic chain. However, maple strongly accumulates Pb in the leaf blade and it is susceptible to degrade quickly due to its high hemicelluloses and cellulose contents and low lignin content. The black locust accumulates metals mainly in the petiole (little consumed by detritivorous). However, its exotic character and high lignin content may limit the interest of its use. |
2018Journal Article ER4 Auteurs : Leclercq-Dransart, Julie; Santorufo, Lucia; Pernin, Céline; Louvel, Brice; Demuynck, Sylvain; Grumiaux, Fabien; Douay, Francis; Leprêtre, Alain Litter breakdown as a tool for assessment of the efficiency of afforestation and ash-aided phytostabilisation on metal contaminated soils functioning in Northern France In: Environmental Science and Pollution Research, 2018, (ACL). @article{Leclercq-Dransart2018, The main objective of the study was to assess the efficiency of phytoremediation methods implemented for 14 years on highly metal contaminated soils. The different experimental strategies were plots planted with a tree mix or with a single tree species coupled or not with the use of fly-ashes as an amendment to limit metals mobility in soil. The breakdown of poplar litter on the four plots was monitored during 10 months. In parallel, colonization of litter bags by functional groups of mesofauna (Collembola and Acari) was followed. Two mesh sized litter-bags were used to allow distinguishing microbial and mesofaunal actions on the litter breakdown. We observed the breakdown of litter in four studied plots. Litter breakdown occurred faster in 3 mm litter bags than 250 µm ones during summer demonstrating the importance of mesofauna. Mixed plantation allowed faster litter breakdown than mono-specific plantation. A higher abundance of mesofauna and/or better abiotic conditions (moisture, shading…) could explain this result. Regarding litter breakdown and mesofauna, no significant difference was observed between the amended plots and those subjected to soil phytomanagement. However, communities of the studied area are disturbed since a low abundance of detritivores was observed. This could explain also the slower litter breakdown than expected in our study. To conclude, among the phytomanagement methods tested, mixed plantations could provide a benefit for the restoration of degraded soils. By contrast, the use of fly-ashes does not seem to have any effect on the functionality of ecosystem neither on the litter breakdown process nor on the abundance of mesofauna. |
2017Conference ER4 Auteurs : Franchomme, Magalie; Audouit, Christelle; Benchendikh, François; Castex, Elodie; Delmer, Sylvie; Demuynck, Sylvain; Grumiaux, Fabien; Hinnewinkel, Christelle; Laboureur, Marie; Litot, Jean-Baptiste; Pernin, Céline; Schmitt, Guillaume; Thierry, Clémentine Mise en synergie des réseaux : évaluer la contribution des emprises des infrastrucutres linéaires de RTE et de SNCF réseau aux continuités écologiques Séminaire ITTECOP, 19-20 octobre 2017, Paris, 2017, (AFF). @conference{Franchomme2017, |
2017Conference ER4 Auteurs : Leclercq-Dransart, Julie; Demuynck, Sylvain; Grumiaux, Fabien; Lemière, Sébastien; Pernin, Céline; Leprêtre, Alain Agir sur la « Trame brune » pour favoriser la mobilité de la faune du sol Journée de lancement du programme CPER Climibio, 6 septembre 2017, Lille, 2017, (AFF). @conference{Leclercq-Dransart2017, |
2017Conference ER4 Auteurs : Leclercq-Dransart, Julie; Demuynck, Sylvain; Grumiaux, Fabien; Lemière, Sébastien; Pernin, Céline; Leprêtre, Alain Les isopodes terrestres face au dérèglement climatique 6ème journée du groupe de travail « TEBIS » (Traits Ecologiques et Biologiques des organismes des Sols), 2-4 octobre 2017, Metz, 2017, (COM). @conference{Leclercq-Dransart2017a, |
2017Conference ER4 Auteurs : Leclercq-Dransart, Julie; Demuynck, Sylvain; Grumiaux, Fabien; Lemière, Sébastien; Pernin, Céline; Leprêtre, Alain Les isopodes terrestres face aux changements climatiques Journée de lancement du programme CPER Climibio, 6 septembre 2017, Lille, 2017, (AFF). @conference{Leclercq-Dransart2017b, |
2017Conference ER4 Auteurs : Leclercq-Dransart, Julie; Santorufo, Lucia; Pernin, Céline; Louvel, Brice; Demuynck, Sylvain; Grumiaux, Fabien; Douay, Francis; Leprêtre, Alain Efficacité de méthodes de phytomanagement sur la fonctionnalité de sols fortement contaminés par les métaux Société Française d'Ecotoxicologie Fondamentale et Appliquée, 29-30 juin 2017, Lille, 2017, (ACTN). @conference{Leclercq-Dransart2017c, Le site atelier metaleurop situé dans l'ancien bassin minier du nord - pas de calais, présente des paysages fortement dégradés par les activités humaines (urbanisation, activités industrielles, agriculture intensive). la forte contamination des sols en métaux aux alentours de l’ancienne fonderie metaleurop nord est bien connue et plusieurs études ont été menées sur le secteur (e.g: comportement des métaux dans le sol et phytomanagement [1,2], risques sanitaires [3] et communautés biologiques [4,5]). une expérimentation a été mise en place sur le terrain pendant 10 mois avec pour objectif d'évaluer l'efficacité de méthodes de remédiation mises en place depuis 17 ans sur des sols contaminés en métaux et fortement dégradés. il s'agit de plantations mixtes ou mono-spécifiques couplées ou non à l'utilisation de cendres volantes comme amendement pour limiter la mobilité des métaux dans le sol. l'approche mise en oeuvre est basée sur le suivi de la dégradation d'une litière de peuplier selon les différents modes de gestion et sa colonisation par les organismes de la mésofaune, connus pour intervenir lors de la dégradation de la matière organique et fournir de nombreux services écosystémiques [6]. la technique des sacs de litière a été utilisée. les principaux résultats montrent que la dégradation de la litière est significativement plus rapide sur la plantation mixte que la monospécifique en été ce qui pourrait s'expliquer par une plus forte abondance de la mésofaune et/ou des conditions abiotiques (humidité, ombrage…) plus favorables à l'activité de celle-ci. en revanche, aucune différence significative n'a été observée entre les parcelles amendées (dégradation de la litière apportée et communautés mésofauniques) et celle soumise au seul phytomanagement. on note que les communautés sont perturbées et que l'abondance des organismes détritivores est faible ce qui pourrait expliquer une dégradation des litières plus lente qu'attendue. ainsi, parmi les méthodes de phytomanagement testées sur le site expérimental, les plantations mixtes pourraient apporter un bénéfice pour la restauration des sols dégradés. l'emploi de cendres volantes n'a pas d'incidence notable sur la fonctionnalité des sols. |
2016Journal Article ER4 Auteurs : Demuynck, Sylvain; Lebel, Aurélie; Grumiaux, Fabien; Pernin, Céline; Leprêtre, Alain; Lemière, Sébastien Comparative avoidance behaviour of the earthworm Eisenia fetida towards chloride, nitrate and sulphate salts of Cd, Cu and Zn using filter paper and extruded water agar gels as exposure media In: Ecotoxicology and Environmental Safety, vol. 129, pp. 66-74, 2016, (ACL). @article{Demuynck2016, We studied the avoidance behaviour of the earthworm eisenia fetida towards cd, cu, and zn, trace elements (tes) tested as chloride, nitrate and sulphate salts. sub adults were exposed individually using dual-cell chambers at 20+2 degrees c in the dark. recordings were realised at different dates from 2 h to 32 h. we used filter paper and extruded water agar gel as exposure media to evaluate the contribution of the dermal and the digestive exposure routes on the avoidance reactions. exposures to cu or cd (10 mg metal ion l-1) resulted in highly significant avoidance reactions through the exposure duration. worms avoided zn poorly and reactions towards zn salts varied along the exposure. worm sensitivity towards tes differed between salts and this could result from differential toxicity or accessibility of these te salts to earthworms. the anion in itself was not the determinant of the avoidance reactions since exposures to similar concentrations of these anions using calcium salts did not result in significant avoidance worm behaviour. avoidance responses towards tes were higher in the case of water agar exposures than in filter paper exposures. thus, dermal contacts with te solutions would elicit worm avoidance but signals from receptors located inside the digestive tract could reinforce this behaviour. the use of extruded water agar gels as the substrate allows checking the real sensitivity of earthworm species towards tes since the te concentrations leading to significant avoidance reactions were below those reported in the literature when using te-spiked soils. (c) 2016 elsevier inc all rights reserved. |
2016Journal Article ER4 Auteurs : Slaby, Sylvain; Lemière, Sébastien; Hanotel, Julie; Lescuyer, Arlette; Demuynck, Sylvain; Bodart, Jean-François; Leprêtre, Alain; Marin, Matthieu Cadmium but not lead exposure affects Xenopus laevis fertilization and embryo cleavage In: Aquatic Toxicology, vol. 177, pp. 1-7, 2016, (ACL). @article{Slaby2016a, Among the toxicological and ecotoxicological studies, few have investigated the effects on germ cells, gametes or embryos, while an impact at these stages will result in serious damage at a population level. thus, it appeared essential to characterize consequences of environmental contaminant exposures at these stages. therefore, we proposed to assess the effects of exposure to cadmium and lead ions, alone or in a binary mixture, on early stages of xenopus laevis life cycle. fertilization and cell division during segmentation were the studied endpoints. cadmium ion exposures decreased in the fertilization rates in a concentration-dependent manner, targeting mainly the oocytes. exposure to this metal ions induced also delays or blockages in the embryonic development. for lead ion exposure, no such effect was observed. for the exposure to the mixture of the two metal ions, concerning the fertilization success, we observed results similar to those obtained with the highest cadmium ion concentration |
2015Journal Article ER4 Auteurs : Grumiaux, Fabien; Demuynck, Sylvain; Pernin, Céline; Leprêtre, Alain Earthworm populations of highly metal-contaminated soils restored by fly ash-aided phytostabilisation In: Ecotoxicology and Environmental Safety, vol. 113, pp. 183-190, 2015, (ACL). @article{Grumiaux2015, Highly metal contaminated soils found in the north of france are the result of intense industrial past. these soils are now unfit for the cultivation of agricultural products for human consumption. solutions have to be found to improve the quality of these soils, and especially to reduce the availability of trace elements (tes). phytostabilisation and ash-aided phytostabilisation applied since 2000 to an experimental site located near a former metallurgical site (metaleurop-nord) was shown previously as efficacious in reducing tes mobility in soils. the aim of the study was to check whether this ten years trial had influenced earthworm communities. this experimental site was compared to plots located in the surroundings and differing by the use of soils. main results are that: (1) whatever the use of soils, earthworm communities are composed of few species with moderate abundance in comparison with communities found in similar habitats outside the tes-contaminated area, (2) the highest abundance and specific richness (4-5 species) were observed in afforested plots with various tree species, (3) ash amendments in afforested plots did not increase the species richness and modified the communities favoring anecic worms but disfavoring epigeic ones. these findings raised the questions of when and how to perform the addition of ashes firstly, to avoid negative effects on soil fauna and secondly, to keep positive effects on metal immobilization. (c) 2014 elsevier inc. all rights reserved. |
2015Journal Article ER4 Auteurs : Marin, Matthieu; Slaby, Sylvain; Marchand, Guillaume; Demuynck, Sylvain; Friscourt, Noémie; Gelaude, Armance; Lemière, Sébastien; Bodart, Jean-François Xenopus laevis oocyte maturation is affected by metal chlorides In: Toxicology in Vitro, vol. 29, no. 5, pp. 1124-1131, 2015, (ACL). @article{Marin2015, Few studies have been conducted using xenopus laevis germ cells as oocytes, though these cells offer many advantages allowing both electrophysiological studies and morphological examination. our aim was to investigate the effects of metal (cadmium, lead, cobalt and zinc) exposures using cell biology approaches. first, cell survival was evaluated with both phenotypical and electrophysiological approaches. secondly, the effect of metals on oocyte maturation was assessed with morphological observations and electrophysiological recordings. from survival experiments, our results showed that metal chlorides did not affect cell morphology but strongly depolarized x. laevis oocyte resting potential. in addition, cadmium chloride was able to inhibit progesterone-induced oocyte maturation. by contrast, zinc, but also to a lesser extent cadmium, cobalt and lead, were able to enhance spontaneous oocyte maturation in the absence of progesterone stimulation. finally, electrophysiological recordings revealed that some metal chlorides (lead, cadmium) exposures could disturb calcium signaling in x. laevis oocyte by modifying calcium-activated chloride currents. our results demonstrated the high sensitivity of x. laevis oocytes toward exogenous metals such as lead and cadmium. in addition, the cellular events recorded might have a predictive value of effects occurring later on the ability of oocytes to be fertilized. together, these results suggest a potential use of this cellular lab model as a tool for ecotoxicological assessment of contaminated fresh waters. |
2015Conference ER4 Auteurs : Slaby, Sylvain; Lemière, Sébastien; Lescuyer, Arlette; Demuynck, Sylvain; Bodart, Jean-François; Marin, Matthieu Effects of cadmium on maturation, fertilization and development of Xenopus laevis oocyte SETAC, 3-7 May 2015, Barcelone (Espagne), 2015, (AFF). @conference{Slaby2015, Through their activities, humans contaminate their environment, by discharging intentionally or not organic or inorganic pollutants. the latter cause adverse effects on the ecosystems and this pollution contribute to the major causes of the global decline of biodiversity, including amphibians. indeed, the aquatic compartments are particularly affected by anthropogenic contaminations. the aim of this work was to highlight the effect of metallic environmental exposure on xenopus laevis oocytes. cadmium (cdcl2), an inorganic compound with natural and mainly anthropogenic sources, such as metallurgy, was chosen. we first focused on cell survival with phenotypical (morphology, pigmentation), physiological (maturation ability) and electrophysiological (resting potential) approaches. then, maturation was assessed in vitro, with morphological (migration and breakdown of the germinal vesicle – gvbd), biochemical (activation of mapk – mitogen activated protein kinase – and mpf – m-phase promoting factor – pathways) and histological (meiotic spindle formation, polar body extrusion) aspects. finally, fertilization and early stages of development (up to one week) were studied. in another work, we demonstrated that chloride metal exposure affects oocyte survival with both morphological and electrophysiological methods. here, oocytes survival was considered by a new physiological method: the ability to undergo maturation after hormonal (progesterone) stimulation i.e. the gain for the oocytes to be competent for fertilization. in a second set of experiments, after cadmium exposures, we demonstrated that mpf pathway (associated with oocyte maturation) is deregulated. fertilization was then assessed when male and female gametes were, together or separately, exposed to cadmium. our data showed that cadmium exposure was responsible for adverse effects in a dose dependent manner on in vitro fertilization rates, development and organogenesis. in addition, our results showed that cadmium preferentially targeted the female gamete rather than spermatozoon, and prevented them to be fertilized. taken together, our results demonstrated that early stages of amphibians could be critical windows of exposure to cadmium. |
2015Conference ER4 Auteurs : Slaby, Sylvain; Lemière, Sébastien; Marchand, Guillaume; Demuynck, Sylvain; Friscourt, Noémie; Gelaude, Armance; Bodart, Jean-François; Marin, Matthieu Xenopus laevis oocyte maturation is affected by metal chlorides SETAC 3-7 May 2015, Barcelone (Espagne), 2015, (ACTI). @conference{Slaby2015a, |
2014Journal Article ER4 Auteurs : Demuynck, Sylvain; Rosalia-Succiu, Iuliana; Grumiaux, Fabien; Douay, Francis; Leprêtre, Alain Effects of field metal-contaminated soils submitted to phytostabilisation and fly ash-aided phytostabilisation on the avoidance behaviour of the earthworm Eisenia fetida In: Ecotoxicology and Environmental Safety, vol. 107, pp. 170-177, 2014, (ACL). @article{Demuynck2014, The earthworm eisenia fetida avoidance behaviour test was used to assess the quality recovery of metal-contaminated soils from lands submitted for 10 years to remediation. soils were from plots located in the surroundings of a former lead smelter plant of northern france. metal concentrations in the soils ranged from 93 to 1231, 56 to 1424, 0.3 to 20 and 15 to 45.5 mg metal/kg dry soil for pb, zn, cd and cu, respectively. several former agricultural plots were treated either by a single phytostabilisation process involving the plantation of a tree mix or by fly ash aided-phytostabilisation. silico-aluminous or sulfo-calcic ashes used were ploughed up to a 25- to 30-cm soil depth at a rate of 23.3 kg/m2 (i.e., 6 percent w/w). e. fetida was shown to avoid significantly the 10 years ash-treated soils whose habitat function has to be considered as limited. this avoidance would relate to a change of the texture of soils induced by the addition of ashes and consisting in an increased level of fine silts together with a decreased level of clays. by contrast, afforested metal-contaminated soils appeared for e. fetida as more attractive than unplanted ones. regarding the influence of the metal contamination of the soils on e. fetida, none of the soils tested even the highest contaminated one was significantly avoided by worms. this lack of reaction would result from the low bioavailability of the metals in the soils tested. at the lights of our results and those previously published on both these ashes and these ash-treated soils, the usefulness of these soil treatments is discussed |
2014Conference ER4 Auteurs : Leclercq-Dransart, Julie; Santorufo, Lucia; Pernin, Céline; Demuynck, Sylvain; Louvel, Brice; Grumiaux, Fabien; Détriché, Sébastien; Douay, Francis; Leprêtre, Alain Can mulching boost ecological connectivity between different management options in a disturbed environment? First Global Soil Biodiversity Conference - Assessing soil biodiversity and its role for ecosystem services, 2-5 décembre 2014, Dijon, 2014, (ACTI). @conference{Leclercq2014a, Today, rare are unfragmented habitats. the landscapes are transformed by human activities and species are threatened: isolation, downsizing, reduced trade between populations... the former coal mining area of northern france is not an exception: urbanization, industry, intensive agriculture and metal pollution are the main causes of land fragmentation and disturbance. it is important to look for ways to reconnect these isolated and degraded areas. the present study aimed at evaluating the attractivity of different kind of mulches and their efficiency to promote recolonization of isolated and degrades areas. since march 2013, 18 experimental rows (6m long x 0.5 m width) of mulch were installed between two wood plots (pluri and monospecific plantations) on a surface area which stretch on 4400 m². five kind of mulch (ramial chipped wood (rcw), dead leaves, chopped miscanthus, chopped wheat straw and canvas cover) and a control (bare soil) were tested (3 replicates each). the control consisted in 3 replicated of bare soil. each mulch was characterized by the loss of biomass with litter bag technique, metal concentrations, temporal monitoring of enzymatic activities and organic matter, etc. the attendance of these mulches by pedofauna was followed using both interception and emergence traps. the formers are wet pitfall traps revealing the activity intensity of aboveground fauna. the latter track imagos of soil insects. the trapping of pedofauna was carried out from april to july 2014 using 90 pitfall traps and 78 soil emergence traps (no trap on canvas cover). a general inventory of pedofauna trapped was made, focusing on two groups models: groundbeetles (carabidae) and woodlice (isopoda), which belong to two different trophic groups, predators and saprophageous. first results show that mulches welcome more organisms than bare soil. leaves and rcw are the modalities with the highest abundances. more detailed results will be presented and discussed. |
2014Conference ER4 Auteurs : Leclercq-Dransart, Julie; Pernin, Céline; Louvel, Brice; Demuynck, Sylvain; Grumiaux, Fabien; Pruvot, Christelle; Douay, Francis; Leprêtre, Alain Contribution à la restauration d’une biodiversité dans un contexte péri-industriel fortement dégradé du Nord de la France Rever 5 : REVER et CONCILIER, 5ème journées atelier. 5-6 février 2014, Université de Rouen, 2014, (ACTN). @conference{Leclercq2014, Contexte l’ancien bassin minier du nord - pas de calais, et plus particulièrement le site atelier metaleurop (réseau safir http://www.safir-network.com/site_metaleurop.html), est un secteur constitué de paysages fortement dégradés et morcelés par les activités humaines (urbanisation, activités industrielles, agriculture intensive). la forte contamination des sols en plomb, cadmium et zinc aux alentours de l’ancienne fonderie de plomb metaleurop nord (noyelles-godault) est notoirement connue. elle conduit à de profonds dysfonctionnements des sols et les concentrations en métaux des productions agricoles végétales excèdent souvent les valeurs réglementaires en vigueur pour l’alimentation humaine, voire animale. l’enjeu majeur pour les institutions en charge de la gestion de ces espaces dégradés est de contribuer à leur restauration en vue de réduire d’une part, les dangers pour les populations et l’environnement et d’autre part, de favoriser leur renaturation et une reconquête paysagère. les services de l’etat envisagent de boiser les terres agricoles les plus contaminées pour les exclure de la production alimentaire. c’est une démarche qu’avait entreprise metaleurop nord entre 1997 et 2003, date de sa fermeture. ceci s’est traduit par le boisement d’environ 85 ha parmi les terres agricoles les plus contaminées aux alentours de l’ancienne fonderie. il est projeté d’exclure toute production agricole sur les sols présentant des concentrations en plomb supérieures à 500 mg kg-1. environ 50 ha de terres labourées pourraient changer d’usage et être boisées. objectif général en octobre 2012, un travail de thèse a débuté avec pour objectif de contribuer au développement du phytomanagement sur des sols fortement contaminés par des métaux. il s’agit de favoriser une renaturation et une biodiversité fonctionnelle des sols les plus contaminés en s’inspirant du retour d’expériences acquises sur les sols anciennement boisés. la fonctionnalité des sols sera observée à différentes échelles au regard de différents indicateurs physico-chimiques et biologiques. matériel et méthode plusieurs dispositifs expérimentaux sont mis en place en vue : •de comparer la vitesse de dégradation des matières organiques sur 4 parcelles boisées (mono/pluri espèces, amendements minéraux des sols ou non) à l’aide de 256 sacs de litière et suivi de la pédofaune ; •d’évaluer la restauration d’une connectivité écologique au travers de 6 modalités (bois raméal fragmenté, miscanthus, paille de céréale, feuilles mortes, bâche plastique et témoin) : inventaire mensuel de la pédofaune (grands groupes taxonomiques), suivi de la dégradation des matières organiques et des activités enzymatiques des sols… •d’examiner différents aménagements paysagers sur ce secteur dégradé à l’aide d’un panel d’indicateurs biologiques et physico-chimiques des sols. premiers résultats il a été mis en évidence : une mosaïque de paysages (plantations arborées, bois, cultures, zones résidentielles et d’activités économiques) entre lesquels la transition est souvent brutale ; des caractéristiques pédologiques variables notamment en termes de teneurs en carbonates, d’engorgement des sols par la nappe phréatique et de degré de contamination ; des communautés faunistiques perturbées, bien que la richesse spécifique de certains groupes soit élevée (carabes) ; et aussi l’absence de micro-habitats et de continuité écologique. les premiers résultats seront présentés et discutés. |
2014Conference ER4 Auteurs : Leclercq-Dransart, Julie; Santorufo, Lucia; Pernin, Céline; Demuynck, Sylvain; Louvel, Brice; Grumiaux, Fabien; Pruvot, Christelle; Douay, Francis; Leprêtre, Alain Évaluation de méthodes d’ingénierie écologique visant à restaurer les habitats dégradés: Intérêt du mulching dans la reconnexion écologique Gestion et requalification durable des sites et sols pollués : Expériences en Nord - Pas de Calais, 25-26 septembre 2014, Villeneuve d’Ascq, 2014, (COM). @conference{Leclercq2014d, |
2014Conference ER4 Auteurs : Leclercq-Dransart, Julie; Santorufo, Lucia; Pernin, Céline; Demuynck, Sylvain; Louvel, Brice; Grumiaux, Fabien; Douay, Francis; Leprêtre, Alain Le mulch de miscanthus peut-il présenter un intérêt pour la pédofaune dans un contexte dégradé et contaminé par les éléments traces métalliques? 3èmes Rencontres nationales de la Recherche sur les sites et sols pollués, ADEME, 18-19 novembre 2014, Paris, 2014, (ACTN). @conference{Leclercq2014c, L’urbanisation, les activités industrielles, la pollution métallique, l’agriculture intensive sont les causes principales de la fragmentation et de la perturbation des sols dans l’ancien bassin minier de la région nord-pas de calais. ceux-ci induisent une perte de biodiversité et le fonctionnement du sol s’en trouve altéré. l’objectif de ce travail est d’évaluer l’intérêt du mulch de miscanthus x giganteus pour constituer un milieu attractif pour la pédofaune et favoriser la recolonisation des sols dégradés. en mars 2013, une expérimentation a été mise en place sur deux parcelles boisées. trois modalités de mulch (miscanthus, bois raméal fragmenté (brf), feuilles mortes) et un témoin (sol nu) ont été caractérisées en termes de concentrations en cadmium (cd), plomb (pb) et zinc (zn) ainsi que par les abondances moyennes et totales de la macrofaune. le mulch de miscanthus, bien que moins colonisé par la pédofaune par rapport aux feuilles ou au brf, est toutefois plus favorable qu’un sol nu. il présente des teneurs en cd, pb et zn très inférieures à celles du brf et des feuilles. le miscanthus apparaît alors comme une solution intermédiaire qui pourrait présenter un intérêt pour la pédofaune dans les réflexions de restauration de sols dégradés. |
2014Conference ER4 Auteurs : Leclercq-Dransart, Julie; Santorufo, Lucia; Pernin, Céline; Demuynck, Sylvain; Louvel, Brice; Grumiaux, Fabien; Détriché, Sébastien; Douay, Francis; Leprêtre, Alain The use of indicators to characterize the functioning of soils strongly affected by metallurgical activities First Global Soil Biodiversity Conference - Assessing soil biodiversity and its role for ecosystem services, 2-5 décembre 2014, Dijon, 2014, (ACTI). @conference{Leclercq2014b, The former coal mining field of northern france has been heavily disturbed due to human activities (urbanization, industry and intensive agriculture). this is particularly the case around a lead smelter (metaleurop nord) that emitted into the atmosphere until 2003 considerable quantities of metals. in order to restore this degraded area and to help reducing the environmental hazard, the most contaminated sites were afforested started in 1997. thus, 85 ha were planted around this former industrial plant creating a “green ring”. different tree species and different management options were established, but no study has been conducted on the effects of different tree species and managements on soil quality and functionality. the main aim of this study was to assess soil functionality of several parcels, differing by vegetation cover (species of trees, planting density, etc.), using soil physico-chemical and biological indicators. results would allow proposing a management for the restoration of these degraded environments. the study site consists of 12 plots: nine wooded plots (plantations of ash, poplar, maple or mixed plantations, with or without soil amendment) and three reference soils (agricultural, hedgerow, forest). the general status of trees and the truck diameters measurements were made. in addition, the topsoil was characterized by texture, bulk density, ph, cec, trace metal concentrations, etc. several soil biological indicators belonging to different functional and trophic groups were selected: microbial activity, ergosterol quantification (as estimation of fungal biomass), enzyme activities (laccase and urease) and several groups of pedofauna. indeed, the imagos emergence of soil insects was monitoring. it was conducted from mid-april to july 2014 using three soil emergence traps per plot. |
2014Conference ER4 Auteurs : Poussin, M; Pernin, Céline; Leclercq-Dransart, Julie; Santorufo, Lucia; Richard, F J; Demuynck, Sylvain; Douay, Francis; Leprêtre, Alain; Souty-Grosset, Catherine Woodlice and recolonization of disturbed habitats in the Région Nord-Pas-de-Calais (France) 9th International Symposium of Terrestrial Isopod Biology, 26-30 juin 2014, Poitiers, 2014, (ACTI). @conference{Poussin2014, |
2013Conference ER4 Auteurs : Demuynck, Sylvain; Succiu, I -R; Pernin, Céline; Douay, Francis; Leprêtre, Alain The Eisenia fetida avoidance behaviour test to assess metal-contaminated soil quality recovery after phytostabilisation SETAC Europe 23rd Annual Meeting, 12-16 May 2013, Glasgow, Scotland (UK), 2013, (ACTI). @conference{Demuynck2013, |
2013Conference ER4 Auteurs : Lemière, Sébastien; Marchand, Guillaume; Demuynck, Sylvain; Marin, Matthieu The Xenopus oocyte, a reliable tool in ecotoxicology? SETAC Europe 23rd Annual Meeting, 12-16 May 2013, Glasgow, Scotland (UK), 2013, (AFF). @conference{Lemiere2013c, |
2013Conference ER4 Auteurs : Marin, Matthieu; Lemière, Sébastien; Marchand, Guillaume; Demuynck, Sylvain; Bodart, Jean-François Toxicity of metal chlorides to Xenopus oocytes SEGH 2013 29th International Conference, 8 juillet 2013, Toulouse, 2013, (AFF). @conference{Marin2013, All around the world, amphibians have declined dramatically over the last 30 years. this decline was first thought to be caused only by the destruction of aquatic habitats, but it was more recently also recognized as the result of anthropogenic contamination of surface still waters. consequently, although all amphibian species beneficiate of the status of endangered species, intensive works have been performed to increase knowledge on both cellular events occurring during normal development and effects of contaminants on their life cycle. for instance, fetax (frog embryo teratogenesis assay xenopus) assay aimed at determining the impact of pollutants on the development using the model xenopus laevis, a south african frog reared in laboratory conditions. nevertheless, few studies have been conducted using xenopus germ cells as oocytes. though these cells offer many advantages allowing both electrophysiological studies and morphological examination. the aim of this work was to investigate the effects of metal contaminant (cadmium, lead, cobalt and zinc) exposures, at environmental concentrations, on xenopus oocytes, using cell biology approaches. two different goals were followed. first, cell survival was evaluating with both phenotypical and electrophysiological approaches, and secondly, the effect of metals on oocyte maturation was assessed with morphological observations (germinal vesicle breakdown) and electrophysiological recordings (calcium-activated chloride currents). our results show that metal chlorides did not affect cell morphology but strongly depolarized oocytes resting potential. in addition, cadmium chloride was able to inhibit progesterone-induced oocyte maturation in a dose-dependent manner. by contrast, alone, we demonstrated that cadmium as well as zinc, cobalt and lead, are able to enhance oocyte maturation. finally, electrophysiological recordings revealed that metal chloride exposures could disturb calcium signalling in xenopus oocyte by modifying calcium activated chloride currents. our results obtained with metal chlorides, demonstrated that xenopus laevis oocyte could be an innovating lab model in ecotoxicology |
2013Conference ER4 Auteurs : Pernin, Céline; Grumiaux, Fabien; Demuynck, Sylvain; Lemière, Sébastien; Leprêtre, Alain Does fly ash-aided phytostabilisation of Pb, Cd and ZN highly contaminated soils improve soil fauna communities? SETAC Europe 23rd Annual Meeting, 12-16 May 2013, Glasgow, Scotland (UK), 2013, (AFF). @conference{Pernin2013, |
2012Journal Article ER4 Auteurs : Godet, Jean-Philippe; Demuynck, Sylvain; Waterlot, Christophe; Lemière, Sébastien; Souty-Grosset, Catherine; Douay, Francis; Leprêtre, Alain; Pruvot, Christelle Fluctuating asymmetry analysis on Porcellio scaber (Crustacea, Isopoda) populations living under metals-contaminated woody habitats In: Ecological Indicators, vol. 23, pp. 130-139, 2012, (ACL). @article{Godet2012, This field study aimed at determining the effect of metal-polluted environments on fluctuating asymmetry (fa) level of porcellio scaber as a measure of developmental stability (ds). for this, woodlice and litter were collected on 8 sites of northern france located either away from metal pollution source or near metallurgical smelters. physico-chemical parameters such as ph, c/n ratio and caco(3) were measured in litter since they could have potential effects on woodlouse population. moreover, cd, pb, and zn concentrations in litter were determined to evaluate the contamination degree of each site. the metal body burdens were determined in woodlice to evaluate their impregnation degree. fluctuating asymmetry levels on males and gravid females were performed on 9 traits (the 2nd and the 3rd articles of antennae, the 1st flagellum segment of the antennae, and the merus, carpus and propodus of the 6th and the 7th pereiopods) according (1) to each site and (2) to two habitat types (cf. metal-contaminated and uncontaminated habitats). results showed close correlations between litter metal concentrations and metal body burdens in woodlice. however, contrary to the initial hypothesis that metal pollution would induce an increase of the fa level, this seemed to be lower for individuals from metal-contaminated habitats than for those from uncontaminated habitats, and particularly for males. the role of antennae and pereiopods on the foraging behavior and reproduction processes on woodlice were discussed and could explain these observations. (c) 2012 elsevier ltd. all rights reserved. |
2012Journal Article ER4 Auteurs : Pernin, Céline; Demuynck, Sylvain; Douay, Francis; Grumiaux, Fabien; Guillemand, David; Leprêtre, Alain; Pohu, Alena; Pourrut, Bertrand; Pruvot, Christelle Une restauration de la biodiversité aux alentours de l’ancienne fonderie Metaleurop Nord est-elle envisageable? Quelques pistes... In: Premières journées techniques du réseau SAFIR. 11-12 mai 2012, Roubaix, 2012, (COM). @article{Pernin2012, |
2011Journal Article ER4 Auteurs : Godet, Jean-Philippe; Demuynck, Sylvain; Waterlot, Christophe; Lemière, Sébastien; Souty-Grosset, Catherine; Scheifler, Renaud; Douay, Francis; Leprêtre, Alain; Pruvot, Christelle Growth and metal accumulation in Porcellio scaber exposed to poplar litter from Cd-, Pb- and Zn-contaminated sites In: Ecotoxicology and Environmental Safety, vol. 74, no. 3, pp. 451-458, 2011, (ACL). @article{Godet2011, This study aimed at determining, in the laboratory, the effects of poplar litter collected in woody habitats contaminated by heavy metals on growth and metal accumulation in the terrestrial isopod porcellio scaber. cd, pb, and zn pseudototal and cacl2-extractable concentrations in litter types were determined using aas. juveniles were fed ad libitum, individually, for 28 days with four litter types presenting an increasing gradient of metal contamination. individuals were weighed every week and metal body burdens were determined at the end of the experiment. from the first week until the end of the experiment, a decrease in p. scaber growth related to the increase of metal concentration in litter types was recorded. significant correlations were observed between metal body burdens and metal concentrations in litter types. however, cd accumulation in woodlice appeared to be related to the cd/zn concentration ratio in litter types. all these results showed the potential of weight gain in p. scaber as a suitable indicator for litter quality assessment with ecological relevance. |
2011Conference ER4 Auteurs : Pruvot, Christelle; Godet, Jean-Philippe; Demuynck, Sylvain; Souty-Grosset, Catherine; Douay, Francis; Leprêtre, Alain The use of fluctuating asymmetry analysis to evaluate the effects of metal-pollution (Cd, Pb, Zn) on Porcellio scaber (Crustacea, Isopoda) living in contaminated woody habitats 12th European Ecological Federation Congress. 25-29 September 2011, Avila (Espagne), 2011, (ACTI). @conference{Pruvot2011, |
2010Journal Article ER4 Auteurs : Bernard, Fabien; Brulle, Franck; Douay, Francis; Lemière, Sébastien; Demuynck, Sylvain; Vandenbulcke, Franck Metallic trace element body burdens and gene expression analysis of biomarker candidates in Eisenia fetida, using an ''exposure/depuration'' experimental scheme with field soils In: Ecotoxicology and Environmental Safety, vol. 73, no. 5, pp. 1034-1045, 2010, (ACL). @article{Bernard2010, Smelting plant activities lead to the accumulation of metal trace elements (mtes) in soils. the presence of high concentrations of mtes can generate an environmental stress likely to affect macroinvertebrates living in close soil contact such as the annelida oligochaeta. eisenia fetida, an ecotoxicologically important test species, was successively exposed to two field soils: (1) a highly contaminated agricultural topsoil collected near the former smelter metaleurop nord (noyelles-godault, france) which contaminated surrounding soils by its atmospheric emissions [exposure phase], and then (2) a slightly contaminated topsoil from an urban garden located in the conurbation of lille (wambrechies) [depuration phase]. two analyses were performed during each phase. firstly, the gene expression levels of four biomarker candidates identified in previous studies were analyzed in e. fetida coelomocytes. these candidates are cd-metallothionein, phytochelatin synthase, coactosin-like protein and lysenin. secondly, the body burdens of the following elements cd, pb, zn, cu, fe, ca, and p were measured. moreover, both analyses were also performed in lumbricus rubellus, an annelid species collected from the two tested soil-originating sites. analysis of gene expression and mte body burdens in both species are discussed to: (1) evaluate expression biomarkers; (2) gain insight the detoxification processes and the long-term response to a metallic stress and (3) compare the responses observed in a test species (e. fetida) with the responses of a field species (l. rubellus). (c) 2010 elsevier inc. all rights reserved. |
2010Journal Article ER4 Auteurs : Grumiaux, Fabien; Demuynck, Sylvain; Schikorski, David; Lemière, Sébastien; Leprêtre, Alain Assessing the effects of FBC ash treatments of metal-contaminated soils using life history traits and metal bioaccumulation analysis of the earthworm Eisenia andrei In: Chemosphere, vol. 79, pp. 156-161, 2010, (ACL). @article{Grumiaux2010, Earthworms (eisenia andrei) were exposed, in controlled conditions, to metal-contaminated soils previously treated in situ with two types of fluidized bed combustion ashes. effects on this species were determined by life history traits analysis. metal immobilizing efficiency of ashes was indicated by metal bioaccumulation. ashes-treated soils reduced worm mortality compared to the untreated soil. however, these ashes reduced both cocoon hatching success and hatchlings numbers compared to the untreated soil. in addition, sulfo-calcical ashes reduced or delayed worm maturity and lowered cocoon production compared to silico-alumineous ones. metal immobilizing efficiency of ashes was demonstrated for zn, cu and to a lesser extent pb. only silico-alumineous ashes reduced cd bioaccumulation, although cd was still bioconcentrated. thus, although ash additions to metal-contaminated soils may help in immobilizing metals, their use might result, depending on the chemical nature of ashes, to severe detrimental effects on earthworm reproduction with possible long term consequences to populations. |
2010Conference ER4 Auteurs : Lemière, Sébastien; Godet, Jean-Philippe; Tanguy, Marion; Grumiaux, Fabien; Leroux, Régine; Vandenbulcke, Franck; Demuynck, Sylvain; Pernin, Céline; Pruvot, Christelle; Delplace, Patrick; Douay, Francis; Leprêtre, Alain Indoor microcosm studies for ecotoxicological evaluation of metal contaminated soil remediation agents SETAC Europe 20th Annual Meeting. 23-27 May 2010, Seville (Espagne), 2010, (ACTI). @conference{Lemiere2010a, |
2009Conference ER4 Auteurs : Bernard, Fabien; Brulle, Franck; Douay, Francis; Lemière, Sébastien; Demuynck, Sylvain; Leprêtre, Alain; Vandenbulcke, Franck Metallic Trace Elements body burden and gene expression analysis of biomarker candidates in Eisenia fetida, during an “intox-detox” protocol performed with field soils SETAC Europe 19th Annual Meeting, 31 May-4 June 2009, Göteborg (Suède), 2009, (ACTI). @conference{Bernard2009, |
2009Conference ER4 Auteurs : Lemière, Sébastien; Grumiaux, Fabien; Godet, Jean-Philippe; Tanguy, Marion; Delplace, Patrick; Demuynck, Sylvain; Brulle, Franck; Pruvot, Christelle; Vandenbulcke, Franck; Douay, Francis; Leprêtre, Alain Utilisation de lombricides exposés en microcosms pour évaluer l’intérêt d’agents immobilisants pour la remediation de sols urbains contaminés par les métaux 2ème Rencontres Nationales de la Recherche sur les Sites & Sols Pollués, ADEME. 20-21 octobre 2009, Paris, 2009, (ACTN). @conference{Lemiere2009, |
2008Conference ER4 Auteurs : Lemière, Sébastien; Godet, Jean-Philippe; Grumiaux, Fabien; Delplace, Patrick; Demuynck, Sylvain; Vandenbulcke, Franck; Douay, Francis; Leprêtre, Alain Eisenia fetida responses to metal immobilizing agents for contaminated soil remediation: a microcosm evaluation SETAC Europe 18th Annual Meeting. 25-29 May 2008, Krakow (Pologne), 2008, (ACTI). @conference{Lemiere2008, |
2008Conference ER4 Auteurs : Lemière, Sébastien; Técher, Didier; Delplace, Patrick; Gilliers, C; Demuynck, Sylvain; Vandenbulcke, Franck; Leprêtre, Alain An indoor microcosm study with zebra mussels of metalcontaminated sediment resupension SETAC Europe 18th Annual Meeting, 25-29 May 2008, Krakow (Poland), 2008, (ACTI). @conference{Lemiere2008a, |
2007Journal Article ER4 Auteurs : Grumiaux, Fabien; Demuynck, Sylvain; Schikorski, David; Lemière, Sébastien; Vandenbulcke, Franck; Leprêtre, Alain Effect of fluidized bed combustion ashes used in metal polluted soil remediation on life history traits of the oligochaeta Eisenia andrei In: European Journal of Soil Biology, vol. 43, no. SUPPL. 1, pp. S256-S260, 2007, (ACL). @article{Grumiaux2007, |
2006Conference ER4 Auteurs : Grumiaux, Fabien; Demuynck, Sylvain; Lemière, Sébastien; Vandenbulcke, Franck; Leprêtre, Alain Effect of fluidized bed combustion ashes used in metal polluted soil remediation on life history traits of the oligochaeta Eisenia andrei 8th International Symposium on Earthworm Ecology, 4-9 September 2006, Krakow (Pologne), 2006, (ACTI). @conference{Grumiaux2006, |
2022Journal Article In: Ecological Engineering, vol. 182, pp. 106694, 2022, (ACL). |
2020Journal Article Comparison of the interest of four types of organic mulches to reclaim degraded areas: a field study based on their relative attractiveness for soil macrofauna In: Ecological Engineering, vol. 158, pp. 106066, 2020, (ACL). |
2019Journal Article Does adding fly ash to metal-contaminated soils play a role in soil functionality regarding metal availability, litter quality, microbial activity and the community structure of Diptera larvae? In: Applied Soil Ecology, vol. 138, pp. 99-111, 2019, (ACL). |
2019Journal Article Isopod physiological and behavioral responses to drier conditions: An experiment with four species in the context of global warming In: European Journal of Soil Biology, vol. 90, pp. 22-30, 2019, (ACL). |
2019Journal Article Adverse effects of fly ashes used as immobilizing agents for highly metal-contaminated soils on Xenopus laevis oocytes survival and maturation-a study performed in the north of France with field soil extracts In: Environmental Science and Pollution Research, vol. 27, pp. 3706–3714, 2019, (ACL). |
2019Journal Article Distribution of metals and cell wall compounds in leaf parts of three tree species suitable for the phytomanagement of heavy metal–contaminated soils In: Water Air and Soil Pollution, vol. 230, no. 237, pp. 1-16, 2019, (ACL). |
2018Journal Article Litter breakdown as a tool for assessment of the efficiency of afforestation and ash-aided phytostabilisation on metal contaminated soils functioning in Northern France In: Environmental Science and Pollution Research, 2018, (ACL). |
2017Conference Mise en synergie des réseaux : évaluer la contribution des emprises des infrastrucutres linéaires de RTE et de SNCF réseau aux continuités écologiques Séminaire ITTECOP, 19-20 octobre 2017, Paris, 2017, (AFF). |
2017Conference Agir sur la « Trame brune » pour favoriser la mobilité de la faune du sol Journée de lancement du programme CPER Climibio, 6 septembre 2017, Lille, 2017, (AFF). |
2017Conference Les isopodes terrestres face au dérèglement climatique 6ème journée du groupe de travail « TEBIS » (Traits Ecologiques et Biologiques des organismes des Sols), 2-4 octobre 2017, Metz, 2017, (COM). |
2017Conference Les isopodes terrestres face aux changements climatiques Journée de lancement du programme CPER Climibio, 6 septembre 2017, Lille, 2017, (AFF). |
2017Conference Efficacité de méthodes de phytomanagement sur la fonctionnalité de sols fortement contaminés par les métaux Société Française d'Ecotoxicologie Fondamentale et Appliquée, 29-30 juin 2017, Lille, 2017, (ACTN). |
2016Journal Article Comparative avoidance behaviour of the earthworm Eisenia fetida towards chloride, nitrate and sulphate salts of Cd, Cu and Zn using filter paper and extruded water agar gels as exposure media In: Ecotoxicology and Environmental Safety, vol. 129, pp. 66-74, 2016, (ACL). |
2016Journal Article Cadmium but not lead exposure affects Xenopus laevis fertilization and embryo cleavage In: Aquatic Toxicology, vol. 177, pp. 1-7, 2016, (ACL). |
2015Journal Article Earthworm populations of highly metal-contaminated soils restored by fly ash-aided phytostabilisation In: Ecotoxicology and Environmental Safety, vol. 113, pp. 183-190, 2015, (ACL). |
2015Journal Article Xenopus laevis oocyte maturation is affected by metal chlorides In: Toxicology in Vitro, vol. 29, no. 5, pp. 1124-1131, 2015, (ACL). |
2015Conference Effects of cadmium on maturation, fertilization and development of Xenopus laevis oocyte SETAC, 3-7 May 2015, Barcelone (Espagne), 2015, (AFF). |
2015Conference Xenopus laevis oocyte maturation is affected by metal chlorides SETAC 3-7 May 2015, Barcelone (Espagne), 2015, (ACTI). |
2014Journal Article Effects of field metal-contaminated soils submitted to phytostabilisation and fly ash-aided phytostabilisation on the avoidance behaviour of the earthworm Eisenia fetida In: Ecotoxicology and Environmental Safety, vol. 107, pp. 170-177, 2014, (ACL). |
2014Conference Can mulching boost ecological connectivity between different management options in a disturbed environment? First Global Soil Biodiversity Conference - Assessing soil biodiversity and its role for ecosystem services, 2-5 décembre 2014, Dijon, 2014, (ACTI). |
2014Conference Contribution à la restauration d’une biodiversité dans un contexte péri-industriel fortement dégradé du Nord de la France Rever 5 : REVER et CONCILIER, 5ème journées atelier. 5-6 février 2014, Université de Rouen, 2014, (ACTN). |
2014Conference Évaluation de méthodes d’ingénierie écologique visant à restaurer les habitats dégradés: Intérêt du mulching dans la reconnexion écologique Gestion et requalification durable des sites et sols pollués : Expériences en Nord - Pas de Calais, 25-26 septembre 2014, Villeneuve d’Ascq, 2014, (COM). |
2014Conference Le mulch de miscanthus peut-il présenter un intérêt pour la pédofaune dans un contexte dégradé et contaminé par les éléments traces métalliques? 3èmes Rencontres nationales de la Recherche sur les sites et sols pollués, ADEME, 18-19 novembre 2014, Paris, 2014, (ACTN). |
2014Conference The use of indicators to characterize the functioning of soils strongly affected by metallurgical activities First Global Soil Biodiversity Conference - Assessing soil biodiversity and its role for ecosystem services, 2-5 décembre 2014, Dijon, 2014, (ACTI). |
2014Conference Woodlice and recolonization of disturbed habitats in the Région Nord-Pas-de-Calais (France) 9th International Symposium of Terrestrial Isopod Biology, 26-30 juin 2014, Poitiers, 2014, (ACTI). |
2013Conference The Eisenia fetida avoidance behaviour test to assess metal-contaminated soil quality recovery after phytostabilisation SETAC Europe 23rd Annual Meeting, 12-16 May 2013, Glasgow, Scotland (UK), 2013, (ACTI). |
2013Conference The Xenopus oocyte, a reliable tool in ecotoxicology? SETAC Europe 23rd Annual Meeting, 12-16 May 2013, Glasgow, Scotland (UK), 2013, (AFF). |
2013Conference Toxicity of metal chlorides to Xenopus oocytes SEGH 2013 29th International Conference, 8 juillet 2013, Toulouse, 2013, (AFF). |
2013Conference Does fly ash-aided phytostabilisation of Pb, Cd and ZN highly contaminated soils improve soil fauna communities? SETAC Europe 23rd Annual Meeting, 12-16 May 2013, Glasgow, Scotland (UK), 2013, (AFF). |
2012Journal Article Fluctuating asymmetry analysis on Porcellio scaber (Crustacea, Isopoda) populations living under metals-contaminated woody habitats In: Ecological Indicators, vol. 23, pp. 130-139, 2012, (ACL). |
2012Journal Article Une restauration de la biodiversité aux alentours de l’ancienne fonderie Metaleurop Nord est-elle envisageable? Quelques pistes... In: Premières journées techniques du réseau SAFIR. 11-12 mai 2012, Roubaix, 2012, (COM). |
2011Journal Article Growth and metal accumulation in Porcellio scaber exposed to poplar litter from Cd-, Pb- and Zn-contaminated sites In: Ecotoxicology and Environmental Safety, vol. 74, no. 3, pp. 451-458, 2011, (ACL). |
2011Conference The use of fluctuating asymmetry analysis to evaluate the effects of metal-pollution (Cd, Pb, Zn) on Porcellio scaber (Crustacea, Isopoda) living in contaminated woody habitats 12th European Ecological Federation Congress. 25-29 September 2011, Avila (Espagne), 2011, (ACTI). |
2010Journal Article Metallic trace element body burdens and gene expression analysis of biomarker candidates in Eisenia fetida, using an ''exposure/depuration'' experimental scheme with field soils In: Ecotoxicology and Environmental Safety, vol. 73, no. 5, pp. 1034-1045, 2010, (ACL). |
2010Journal Article Assessing the effects of FBC ash treatments of metal-contaminated soils using life history traits and metal bioaccumulation analysis of the earthworm Eisenia andrei In: Chemosphere, vol. 79, pp. 156-161, 2010, (ACL). |
2010Conference Indoor microcosm studies for ecotoxicological evaluation of metal contaminated soil remediation agents SETAC Europe 20th Annual Meeting. 23-27 May 2010, Seville (Espagne), 2010, (ACTI). |
2009Conference Metallic Trace Elements body burden and gene expression analysis of biomarker candidates in Eisenia fetida, during an “intox-detox” protocol performed with field soils SETAC Europe 19th Annual Meeting, 31 May-4 June 2009, Göteborg (Suède), 2009, (ACTI). |
2009Conference Utilisation de lombricides exposés en microcosms pour évaluer l’intérêt d’agents immobilisants pour la remediation de sols urbains contaminés par les métaux 2ème Rencontres Nationales de la Recherche sur les Sites & Sols Pollués, ADEME. 20-21 octobre 2009, Paris, 2009, (ACTN). |
2008Conference Eisenia fetida responses to metal immobilizing agents for contaminated soil remediation: a microcosm evaluation SETAC Europe 18th Annual Meeting. 25-29 May 2008, Krakow (Pologne), 2008, (ACTI). |
2008Conference An indoor microcosm study with zebra mussels of metalcontaminated sediment resupension SETAC Europe 18th Annual Meeting, 25-29 May 2008, Krakow (Poland), 2008, (ACTI). |
2007Journal Article Effect of fluidized bed combustion ashes used in metal polluted soil remediation on life history traits of the oligochaeta Eisenia andrei In: European Journal of Soil Biology, vol. 43, no. SUPPL. 1, pp. S256-S260, 2007, (ACL). |
2006Conference Effect of fluidized bed combustion ashes used in metal polluted soil remediation on life history traits of the oligochaeta Eisenia andrei 8th International Symposium on Earthworm Ecology, 4-9 September 2006, Krakow (Pologne), 2006, (ACTI). |