2008Article de journal ER4 Auteurs : Béziat, Didier; Dubois, Michel; Debat, Pierre; Nikiéma, Serge; Salvi, Stefano; Tollon, Francis Gold metallogeny in the Birimian craton of Burkina Faso (West Africa) Dans: Journal of African Earth Sciences, vol. 50, no. 2-4, p. 215-233, 2008, (ACL). Résumé @article{Beziat2008,
title = {Gold metallogeny in the Birimian craton of Burkina Faso (West Africa)},
author = {Didier Béziat and Michel Dubois and Pierre Debat and Serge Nikiéma and Stefano Salvi and Francis Tollon},
year = {2008},
date = {2008-01-01},
journal = {Journal of African Earth Sciences},
volume = {50},
number = {2-4},
pages = {215-233},
abstract = {Primary gold deposits in burkina faso occur in paleoproterozoic birimian belt formations (2.0 ga). mineralization was synchronous with regional metamorphism and deformation, and is either hosted within, or is adjacent to, quartz-bearing veins. these are classical characteristics of epigenetic gold deposits in precambrian metamorphic terranes and permit to classify the mineralized sites from burkina faso as orogenic-type gold deposits. a review of data collected over the past decade by our team permits to recognize two main styles of gold mineralization: (1) quartz-vein hosted; this style occurs in all lithologies, the veins are deformed and gold is principally concentrated within the veins, associated with either sulfides or tourmaline. (2) disseminated; this style occurs exclusively in albitites (and to a lesser extent listvenites) with gold occurring mainly within alteration halos of generally undeformed quartz-albite-carbonate vein. quartz-vein and disseminated styles of mineralization can be associated within the same deposit. albitites and listvenites are alteration products of mainly calc-alkaline igneous rocks of felsic to ultramafic composition, respectively. the predominant alteration assemblage consists of chlorite, albite, carbonate, and pyrite. sulfides occur as fine masses commonly in the alteration halos close to vein margins and consist mainly of pyrite and arsenopyrite, depending on host-rock composition. gold occurs as free native metal and, locally, in form of tellurides, in fissures or as inclusions within pyrite and arsenopyrite. two main populations of fluid inclusions are associated with the gold deposits, independently of the mineralization style: (1) carbonic inclusions consisting of up to 90 mol% co2 (plus n2 and ch4) and (2) aqueous-carbonic fluid inclusions with moderate salinities. interestingly, the disseminated gold style deposits of burkina faso, which have the highest economic potential, show strong similarities with the world-class ashanti deposit, in neighboring ghana.},
note = {ACL},
keywords = {ER4},
pubstate = {published},
tppubtype = {article}
}
Primary gold deposits in burkina faso occur in paleoproterozoic birimian belt formations (2.0 ga). mineralization was synchronous with regional metamorphism and deformation, and is either hosted within, or is adjacent to, quartz-bearing veins. these are classical characteristics of epigenetic gold deposits in precambrian metamorphic terranes and permit to classify the mineralized sites from burkina faso as orogenic-type gold deposits. a review of data collected over the past decade by our team permits to recognize two main styles of gold mineralization: (1) quartz-vein hosted; this style occurs in all lithologies, the veins are deformed and gold is principally concentrated within the veins, associated with either sulfides or tourmaline. (2) disseminated; this style occurs exclusively in albitites (and to a lesser extent listvenites) with gold occurring mainly within alteration halos of generally undeformed quartz-albite-carbonate vein. quartz-vein and disseminated styles of mineralization can be associated within the same deposit. albitites and listvenites are alteration products of mainly calc-alkaline igneous rocks of felsic to ultramafic composition, respectively. the predominant alteration assemblage consists of chlorite, albite, carbonate, and pyrite. sulfides occur as fine masses commonly in the alteration halos close to vein margins and consist mainly of pyrite and arsenopyrite, depending on host-rock composition. gold occurs as free native metal and, locally, in form of tellurides, in fissures or as inclusions within pyrite and arsenopyrite. two main populations of fluid inclusions are associated with the gold deposits, independently of the mineralization style: (1) carbonic inclusions consisting of up to 90 mol% co2 (plus n2 and ch4) and (2) aqueous-carbonic fluid inclusions with moderate salinities. interestingly, the disseminated gold style deposits of burkina faso, which have the highest economic potential, show strong similarities with the world-class ashanti deposit, in neighboring ghana. |
2008Article de journal ER4 Auteurs : Bidar, Géraldine; Verdin, Anthony; Garçon, Guillaume; Pruvot, Christelle; Laruelle, Frédéric; Grandmougin-Ferjani, Anne; Douay, Francis; Shirali, Pirouz Changes in fatty acid composition and content of two plants (Lolium perenne and Trifolium repens) grown during 6 and 18 months in a metal (Pb, Cd, Zn) contaminated field Dans: Water Air and Soil Pollution, vol. 192, no. 1-4, p. 281-291, 2008, (ACL). Résumé @article{Bidar2008,
title = {Changes in fatty acid composition and content of two plants (Lolium perenne and Trifolium repens) grown during 6 and 18 months in a metal (Pb, Cd, Zn) contaminated field},
author = {Géraldine Bidar and Anthony Verdin and Guillaume Garçon and Christelle Pruvot and Frédéric Laruelle and Anne Grandmougin-Ferjani and Francis Douay and Pirouz Shirali},
year = {2008},
date = {2008-01-01},
journal = {Water Air and Soil Pollution},
volume = {192},
number = {1-4},
pages = {281-291},
abstract = {The aim of this in situ study was to investigate the fatty acid (fa) composition and content in roots and shoots of lolium perenne and trifolium repens, grown under heavy metal stress (cd, pb, zn). the composition of fa was quite similar for the two plants and the two organs; main fa were palmitic acid (c16:0), oleic acid (c18:1), linoleic acid (c18:2) and linolenic acid (c18:3). for both plants, the major fa that characterized the roots was c18:2 whereas c18:3 was the prominent fa in shoots. for the first sampling (s1), in the roots of l. perenne and t. repens, polyunsaturated fatty acids (pufa) were affected by metal contamination while, in the second sampling (s2), pufa were affected in the shoots of the two plants. this alteration of pufa was well correlated with the bioaccumulation factor of metals which decreased in roots and increased in shoots with the time. moreover, a positive correlation was found between the pufa decrease and the malondialdehyde (mda) content, indicating the occurrence of a lipid peroxidation induced by the metal stress},
note = {ACL},
keywords = {ER4},
pubstate = {published},
tppubtype = {article}
}
The aim of this in situ study was to investigate the fatty acid (fa) composition and content in roots and shoots of lolium perenne and trifolium repens, grown under heavy metal stress (cd, pb, zn). the composition of fa was quite similar for the two plants and the two organs; main fa were palmitic acid (c16:0), oleic acid (c18:1), linoleic acid (c18:2) and linolenic acid (c18:3). for both plants, the major fa that characterized the roots was c18:2 whereas c18:3 was the prominent fa in shoots. for the first sampling (s1), in the roots of l. perenne and t. repens, polyunsaturated fatty acids (pufa) were affected by metal contamination while, in the second sampling (s2), pufa were affected in the shoots of the two plants. this alteration of pufa was well correlated with the bioaccumulation factor of metals which decreased in roots and increased in shoots with the time. moreover, a positive correlation was found between the pufa decrease and the malondialdehyde (mda) content, indicating the occurrence of a lipid peroxidation induced by the metal stress |
2008Article de journal ER4 Auteurs : Brulle, Franck; Cocquerelle, Claude; Mitta, Guillaume; Castric, Vincent; Douay, Francis; Leprêtre, Alain; Vandenbulcke, Franck Identification and expression profile of gene transcripts differentially expressed during metallic exposure in Eisenia fetida coelomocytes Dans: Developmental and Comparative Immunology, vol. 32, p. 1441-1453, 2008, (ACL). Résumé @article{Brulle2008,
title = {Identification and expression profile of gene transcripts differentially expressed during metallic exposure in Eisenia fetida coelomocytes},
author = {Franck Brulle and Claude Cocquerelle and Guillaume Mitta and Vincent Castric and Francis Douay and Alain Leprêtre and Franck Vandenbulcke},
year = {2008},
date = {2008-01-01},
journal = {Developmental and Comparative Immunology},
volume = {32},
pages = {1441-1453},
abstract = {The aim of this work was to identify in eisenia fetida genes whose expression are regulated following exposure to a complex mixture of metallic trace elements (mte) representative of a highly polluted smelter soil. suppression subtractive hybridization (ssh) was used to construct cdna libraries enriched in up- or down-regulated transcripts in the immunecirculating cells of the coelomic cavities, namely coelomocytes, from worms exposed to metallic pollution. among 1536 ssh-derived cdna clones sequenced, we identified 764 unique ests of which we selected 18 candidates on the basis of their redundancy. these selected candidates were subjected to a two-step validation procedure based on the study of their expression level by real-time pcr. the first step consisted in measuring the expression of the 18 candidates in worms exposed to artificial contaminated soil. the second step consisted in measuring the expression in animals exposed to a ‘‘naturally’’ contaminated soil sampled close to a smelter. both steps allowed us to highlight 3 candidates that are strongly induced in worms exposed to a smelter polluted soil. these candidates are: the well-known mte-induced cd–metallothionein and 2 original biomarkers, lysenin, and a transcript, which cloning of the complete coding sequence identified as the coactosin-like protein.},
note = {ACL},
keywords = {ER4},
pubstate = {published},
tppubtype = {article}
}
The aim of this work was to identify in eisenia fetida genes whose expression are regulated following exposure to a complex mixture of metallic trace elements (mte) representative of a highly polluted smelter soil. suppression subtractive hybridization (ssh) was used to construct cdna libraries enriched in up- or down-regulated transcripts in the immunecirculating cells of the coelomic cavities, namely coelomocytes, from worms exposed to metallic pollution. among 1536 ssh-derived cdna clones sequenced, we identified 764 unique ests of which we selected 18 candidates on the basis of their redundancy. these selected candidates were subjected to a two-step validation procedure based on the study of their expression level by real-time pcr. the first step consisted in measuring the expression of the 18 candidates in worms exposed to artificial contaminated soil. the second step consisted in measuring the expression in animals exposed to a ‘‘naturally’’ contaminated soil sampled close to a smelter. both steps allowed us to highlight 3 candidates that are strongly induced in worms exposed to a smelter polluted soil. these candidates are: the well-known mte-induced cd–metallothionein and 2 original biomarkers, lysenin, and a transcript, which cloning of the complete coding sequence identified as the coactosin-like protein. |
2008Conférence ER4 Auteurs : Brulle, Franck; Cocquerelle, Claude; Mitta, Guillaume; Castric, Vincent; Douay, Francis; Leprêtre, Alain; Vandenbulcke, Franck Identification and expression profile of gene transcripts differentially expressed during metallic exposure in Eisenia fetida SETAC Europe 18th Annual Meeting, 25-29 May 2008, Krakow (Poland), 2008, (ACTI). @conference{Brulle2008b,
title = {Identification and expression profile of gene transcripts differentially expressed during metallic exposure in Eisenia fetida},
author = {Franck Brulle and Claude Cocquerelle and Guillaume Mitta and Vincent Castric and Francis Douay and Alain Leprêtre and Franck Vandenbulcke},
year = {2008},
date = {2008-01-01},
booktitle = {SETAC Europe 18th Annual Meeting, 25-29 May 2008, Krakow (Poland)},
note = {ACTI},
keywords = {ER4},
pubstate = {published},
tppubtype = {conference}
}
|
2008Conférence ER4 Auteurs : Brulle, Franck; Cocquerelle, Claude; Mitta, Guillaume; Lemière, Sébastien; Leprêtre, Alain; Vandenbulcke, Franck Identification and analysis of the expression profiles of new biomarker candidates to gain insight the immune response to a metallic stress SETAC Europe 18th Annual Meeting. 25-29 May 2008, Krakow (Pologne), 2008, (COM). @conference{Brulle2008c,
title = {Identification and analysis of the expression profiles of new biomarker candidates to gain insight the immune response to a metallic stress},
author = {Franck Brulle and Claude Cocquerelle and Guillaume Mitta and Sébastien Lemière and Alain Leprêtre and Franck Vandenbulcke},
year = {2008},
date = {2008-01-01},
booktitle = {SETAC Europe 18th Annual Meeting. 25-29 May 2008, Krakow (Pologne)},
note = {COM},
keywords = {ER4},
pubstate = {published},
tppubtype = {conference}
}
|
2008Article de journal ER4 Auteurs : Brulle, Franck; Cocquerelle, Claude; Wamalah, Atta-Nda; Morgan, Andrew-John; Kille, Peter; Leprêtre, Alain; Vandenbulcke, Franck CDNA cloning and expression analysis of Eisenia fetida (Annelida:Oligochaeta) phytochelatin synthase under cadmium exposure Dans: Ecotoxicology and Environmental Safety, vol. 71, no. 1, p. 47-55, 2008, (ACL). Résumé @article{Brulle2008a,
title = {CDNA cloning and expression analysis of Eisenia fetida (Annelida:Oligochaeta) phytochelatin synthase under cadmium exposure},
author = {Franck Brulle and Claude Cocquerelle and Atta-Nda Wamalah and Andrew-John Morgan and Peter Kille and Alain Leprêtre and Franck Vandenbulcke},
year = {2008},
date = {2008-01-01},
journal = {Ecotoxicology and Environmental Safety},
volume = {71},
number = {1},
pages = {47-55},
abstract = {Metallothioneins (mts) are central to trace metal homeostasis and detoxification throughout biological systems. prokaryotes, plants, and fungi utilize both gene encoded cysteine-rich polypeptides (classically designated class i and ii mts) and enzymatically synthesized cysteinerich peptides (classically designated class iii mts or phytochelatins). in contrast, although gene encoded mts are ubiquitous in animal species the identification of a functional phytochelatin synthase in the nematode caenorhabditis elegans, a representative member of the ecdysozoa, provided the first evidence for these metal-binding peptides in animals. by exploiting the conservation observed between species we have been able to clone and transcriptionally characterize a phytochelatin synthase from the immune cells of the earthworm eisenia fetida, the first evidence for its presence in a phylum belonging to the lophototrochozoa. the complete coding sequence of this enzyme was determined and the phylogenetic relationship to plant, yeast and nematode enzymes elucidated. temporal- and dose-profiling of the transcriptional regulation of phytochelatin synthase and mt in response to cadmium was performed by using real-time pcr.},
note = {ACL},
keywords = {ER4},
pubstate = {published},
tppubtype = {article}
}
Metallothioneins (mts) are central to trace metal homeostasis and detoxification throughout biological systems. prokaryotes, plants, and fungi utilize both gene encoded cysteine-rich polypeptides (classically designated class i and ii mts) and enzymatically synthesized cysteinerich peptides (classically designated class iii mts or phytochelatins). in contrast, although gene encoded mts are ubiquitous in animal species the identification of a functional phytochelatin synthase in the nematode caenorhabditis elegans, a representative member of the ecdysozoa, provided the first evidence for these metal-binding peptides in animals. by exploiting the conservation observed between species we have been able to clone and transcriptionally characterize a phytochelatin synthase from the immune cells of the earthworm eisenia fetida, the first evidence for its presence in a phylum belonging to the lophototrochozoa. the complete coding sequence of this enzyme was determined and the phylogenetic relationship to plant, yeast and nematode enzymes elucidated. temporal- and dose-profiling of the transcriptional regulation of phytochelatin synthase and mt in response to cadmium was performed by using real-time pcr. |
2008Article de journal ER4 Auteurs : Carlier, Erick Analytical solutions of the advection-dispersion equation for transient groundwater flow. A numerical validation Dans: Hydrological Processes, vol. 22, p. 3500-3506, 2008, (ACL). Résumé @article{Carlier2008,
title = {Analytical solutions of the advection-dispersion equation for transient groundwater flow. A numerical validation},
author = {Erick Carlier},
year = {2008},
date = {2008-01-01},
journal = {Hydrological Processes},
volume = {22},
pages = {3500-3506},
abstract = {An analytical transport-model was developed to simulate the propagation of a contaminant in one- and two-dimensional transient flow in groundwater. it is proved that the distribution of concentration at a given time and for a given discharge is identical to that obtained for a different discharge if the volumetric flux of water is the same in the two cases. the results of simulations have been compared with results obtained using the mt3dms numerical model. there is good agreement when the calculated concentrations are flux-weighted concentrations. on the other hand, there is a notable divergence when the resident mode is considered. resident mode concentrations express the mass per unit volume whereas flux mode concentrations express the ratio of mass flux to fluid flux. the solutions presented in this paper can thus be a useful alternative to code mt3dms when the objective is to simulate concentrations in transient flow according to a resident mode},
note = {ACL},
keywords = {ER4},
pubstate = {published},
tppubtype = {article}
}
An analytical transport-model was developed to simulate the propagation of a contaminant in one- and two-dimensional transient flow in groundwater. it is proved that the distribution of concentration at a given time and for a given discharge is identical to that obtained for a different discharge if the volumetric flux of water is the same in the two cases. the results of simulations have been compared with results obtained using the mt3dms numerical model. there is good agreement when the calculated concentrations are flux-weighted concentrations. on the other hand, there is a notable divergence when the resident mode is considered. resident mode concentrations express the mass per unit volume whereas flux mode concentrations express the ratio of mass flux to fluid flux. the solutions presented in this paper can thus be a useful alternative to code mt3dms when the objective is to simulate concentrations in transient flow according to a resident mode |
2008Article de journal ER4 Auteurs : Descamps, Michel; Duhoo, T; Monchau, Francine; Lu, J; Hardouin, P; Hornez, J C; Leriche, A Manufacture of macroporous β-tricalcium phosphate bioceramics Dans: Journal of the European Ceramic Society, vol. 28, no. 1, p. 149-157, 2008, (ACL). Résumé @article{Descamps2008b,
title = {Manufacture of macroporous β-tricalcium phosphate bioceramics},
author = {Michel Descamps and T Duhoo and Francine Monchau and J Lu and P Hardouin and J C Hornez and A Leriche},
year = {2008},
date = {2008-01-01},
journal = {Journal of the European Ceramic Society},
volume = {28},
number = {1},
pages = {149-157},
abstract = {Β-tricalcium phosphate (β-tcp) macroporous ceramics were produced by a new manufacturing procedure. an organic skeleton constituted of polymethylmethacrylate balls (pmma) is carried by a chemical forming treatment. this treatment consists to establish a connection between pmma balls by a chemical superficial dissolution of the individual beads. this reaction is accompanied of significant shrinkage of the organic skeleton which is correlated with the interconnection size between beads. an empirical relation and a geometrical model, based on a theoretical arrangement of spheres of uniform sizes, were developed to determine the necessary shrinkage of the organic skeleton to obtain a desired interconnection size. pmma skeleton is impregnated by using an aqueous suspension of β-tcp, after drying, the ceramic/polymeric composite undergoes the traditional steps of debinding and sintering to obtain finished material. this process allows a total control of the porous architecture of the part (sizes of pores and interconnections) and also to perform materials with very varied forms and dimensions with specific properties as a gradient of pore sizes or a gradient of the interconnection sizes.},
note = {ACL},
keywords = {ER4},
pubstate = {published},
tppubtype = {article}
}
Β-tricalcium phosphate (β-tcp) macroporous ceramics were produced by a new manufacturing procedure. an organic skeleton constituted of polymethylmethacrylate balls (pmma) is carried by a chemical forming treatment. this treatment consists to establish a connection between pmma balls by a chemical superficial dissolution of the individual beads. this reaction is accompanied of significant shrinkage of the organic skeleton which is correlated with the interconnection size between beads. an empirical relation and a geometrical model, based on a theoretical arrangement of spheres of uniform sizes, were developed to determine the necessary shrinkage of the organic skeleton to obtain a desired interconnection size. pmma skeleton is impregnated by using an aqueous suspension of β-tcp, after drying, the ceramic/polymeric composite undergoes the traditional steps of debinding and sintering to obtain finished material. this process allows a total control of the porous architecture of the part (sizes of pores and interconnections) and also to perform materials with very varied forms and dimensions with specific properties as a gradient of pore sizes or a gradient of the interconnection sizes. |
2008Article de journal ER4 Auteurs : Détriché, Sébastien; Bréhéret, Jean-Gabriel; Zarki, Hamid; Karrat, L’houcine; Macaire, Jean-Jacques; Fontugne, Michel Late Holocene palaeohydrology of lake Afourgagh (Middle-Atlas, Morocco) from deposit geometry and facies Dans: Bulletin de la Societe Géologique de France, vol. 179, no. 1, p. 41-50, 2008, (ACLN). Résumé @article{Detriche2008b,
title = {Late Holocene palaeohydrology of lake Afourgagh (Middle-Atlas, Morocco) from deposit geometry and facies},
author = {Sébastien Détriché and Jean-Gabriel Bréhéret and Hamid Zarki and L’houcine Karrat and Jean-Jacques Macaire and Michel Fontugne},
year = {2008},
date = {2008-01-01},
journal = {Bulletin de la Societe Géologique de France},
volume = {179},
number = {1},
pages = {41-50},
abstract = {The geometry and facies of deposits in lake afourgagh (middle-atlas, morocco) provide evidence for successive major lake level fluctuations during the last 2,500 years. sedimentation is mainly biogenic with the laying down of thick charophyte-rich tufas interbedded with silty layers in distal areas and palaeosols in proximal areas. these alternations point to four major deposit sequences with lowstand phases. the first two phases are dated at ca. 2426 and 1869 cal b.p. these events appear to be linked to major arid episodes on a regional scale. however, climatic impact has been modulated by human activities, particularly during recent decades.},
note = {ACLN},
keywords = {ER4},
pubstate = {published},
tppubtype = {article}
}
The geometry and facies of deposits in lake afourgagh (middle-atlas, morocco) provide evidence for successive major lake level fluctuations during the last 2,500 years. sedimentation is mainly biogenic with the laying down of thick charophyte-rich tufas interbedded with silty layers in distal areas and palaeosols in proximal areas. these alternations point to four major deposit sequences with lowstand phases. the first two phases are dated at ca. 2426 and 1869 cal b.p. these events appear to be linked to major arid episodes on a regional scale. however, climatic impact has been modulated by human activities, particularly during recent decades. |
2008Article de journal ER4 Auteurs : Douay, Francis; Pruvot, Christelle; Roussel, Hélène; Ciesielski, Henri; Fourrier, Hervé; Proix, Nicolas; Waterlot, Christophe Contamination of urban soils in an area of Northern France polluted by dust emissions of two smelters Dans: Water Air and Soil Pollution, vol. 188, no. 1-4, p. 247-260, 2008, (ACL). Résumé @article{Douay2008,
title = {Contamination of urban soils in an area of Northern France polluted by dust emissions of two smelters},
author = {Francis Douay and Christelle Pruvot and Hélène Roussel and Henri Ciesielski and Hervé Fourrier and Nicolas Proix and Christophe Waterlot},
year = {2008},
date = {2008-01-01},
journal = {Water Air and Soil Pollution},
volume = {188},
number = {1-4},
pages = {247-260},
abstract = {The contamination of 27 urban topsoils has been assessed around two lead and zinc smelters (metaleurop nord and umicore) in the north of france. eighteen trace elements have been analysed (ag, as, bi, cd, co, cr, cu, hg, in, ni, pb, sb, se, sn, tl, th, u and zn). the investigation included the study of the vertical distribution of cd, pb and zn as indicators of pollution. it was shown that cd, in, pb, sb and zn were major pollutants followed in lesser quantities by ag, bi, cu and hg. in addition, as, ni, se, sn and tl were present at levels slightly higher than regional agricultural values. the other elements (co, cr, th and u) were at endogenous levels. the observations have highlighted the strong heterogeneity of the physico-chemical parameters of urban soils and the existence of heavy contamination of the under layers by cd, pb and zn. a potential transfer of metals from the topsoil to the deeper layers and especially cd and zn, is not excluded. indeed the soil rework is not the only factor explaining contamination level of the deeper layers of the studied soils. the comparison of the studied element concentrations in urban soils with nearby local agricultural values shows that the dust emission originating from the metaleurop and umicore smelters were not the only source of contamination. thus a large contamination of the studied urban soils by sb and in could be explained by domestic combustion of coal for heating.},
note = {ACL},
keywords = {ER4},
pubstate = {published},
tppubtype = {article}
}
The contamination of 27 urban topsoils has been assessed around two lead and zinc smelters (metaleurop nord and umicore) in the north of france. eighteen trace elements have been analysed (ag, as, bi, cd, co, cr, cu, hg, in, ni, pb, sb, se, sn, tl, th, u and zn). the investigation included the study of the vertical distribution of cd, pb and zn as indicators of pollution. it was shown that cd, in, pb, sb and zn were major pollutants followed in lesser quantities by ag, bi, cu and hg. in addition, as, ni, se, sn and tl were present at levels slightly higher than regional agricultural values. the other elements (co, cr, th and u) were at endogenous levels. the observations have highlighted the strong heterogeneity of the physico-chemical parameters of urban soils and the existence of heavy contamination of the under layers by cd, pb and zn. a potential transfer of metals from the topsoil to the deeper layers and especially cd and zn, is not excluded. indeed the soil rework is not the only factor explaining contamination level of the deeper layers of the studied soils. the comparison of the studied element concentrations in urban soils with nearby local agricultural values shows that the dust emission originating from the metaleurop and umicore smelters were not the only source of contamination. thus a large contamination of the studied urban soils by sb and in could be explained by domestic combustion of coal for heating. |
2008Article de journal ER4 Auteurs : Douay, Francis; Roussel, Hélène; Pruvot, Christelle; Loriette, Alexandre; Fourrier, Hervé Assessment of a remediation technique using the replacement of contaminated soils in kitchen gardens nearby a former lead smelter in Northern France Dans: Science of the Total Environment, vol. 401, no. 1-3, p. 29-38, 2008, (ACL). Résumé @article{Douay2008a,
title = {Assessment of a remediation technique using the replacement of contaminated soils in kitchen gardens nearby a former lead smelter in Northern France},
author = {Francis Douay and Hélène Roussel and Christelle Pruvot and Alexandre Loriette and Hervé Fourrier},
year = {2008},
date = {2008-01-01},
journal = {Science of the Total Environment},
volume = {401},
number = {1-3},
pages = {29-38},
abstract = {Vegetables cultivated in kitchen gardens that are strongly contaminated by heavy metals (pb, cd) may represent to consumers a means of exposure to these metals. this exposure is more problematic for those families that include a large quantity of home-grown vegetables in their diet. researchers have shown that the majority of vegetables produced in kitchen gardens in the vicinity of the metaleurop nord smelter (northern france) do not conform to european regulations. this study was carried out in three of these kitchen gardens. the concentrations of cd and pb in the topsoils were up to 24 and 3300 mg kg−1 respectively. the method consisted of delineating a surface area of about 50 to 100 m2 for each garden, then removing the contaminated soil and replacing it with a clean one. seven species of vegetables were cultivated from 2003 to 2005 in the original contaminated soils and the remediated ones. the data showed a clear improvement of the quality of the vegetables cultivated in remediated soils, although 17% of them were still over the european legislative limits for foodstuffs. this suggested that there was a foliar contamination due to contaminated dust fallout coming from the closed smelter site and the adjacent polluted soils. in addition, the measurement of the cd and pb concentrations in the dust fallout showed that the substantial rise in metal concentrations in the remediated soil was not only due to atmospheric fallout. these results raise questions about possible technical, economic and sociological problems associated with this kind of remediation.},
note = {ACL},
keywords = {ER4},
pubstate = {published},
tppubtype = {article}
}
Vegetables cultivated in kitchen gardens that are strongly contaminated by heavy metals (pb, cd) may represent to consumers a means of exposure to these metals. this exposure is more problematic for those families that include a large quantity of home-grown vegetables in their diet. researchers have shown that the majority of vegetables produced in kitchen gardens in the vicinity of the metaleurop nord smelter (northern france) do not conform to european regulations. this study was carried out in three of these kitchen gardens. the concentrations of cd and pb in the topsoils were up to 24 and 3300 mg kg−1 respectively. the method consisted of delineating a surface area of about 50 to 100 m2 for each garden, then removing the contaminated soil and replacing it with a clean one. seven species of vegetables were cultivated from 2003 to 2005 in the original contaminated soils and the remediated ones. the data showed a clear improvement of the quality of the vegetables cultivated in remediated soils, although 17% of them were still over the european legislative limits for foodstuffs. this suggested that there was a foliar contamination due to contaminated dust fallout coming from the closed smelter site and the adjacent polluted soils. in addition, the measurement of the cd and pb concentrations in the dust fallout showed that the substantial rise in metal concentrations in the remediated soil was not only due to atmospheric fallout. these results raise questions about possible technical, economic and sociological problems associated with this kind of remediation. |
2008Article de journal ER4 Auteurs : Douay, Francis; Roussel, Hélène; Pruvot, Christelle; Waterlot, Christophe Impact of a smelter closedown on metal contents of wheat cultivated in the neighbourhood Dans: Environmental Science and Pollution Research, vol. 15, p. 162-169, 2008, (ACL). Résumé @article{Douay2008b,
title = {Impact of a smelter closedown on metal contents of wheat cultivated in the neighbourhood},
author = {Francis Douay and Hélène Roussel and Christelle Pruvot and Christophe Waterlot},
year = {2008},
date = {2008-01-01},
journal = {Environmental Science and Pollution Research},
volume = {15},
pages = {162-169},
abstract = {Background. the contamination of soils by heavy metals engenders important environmental and sanitary problems in northern france where a smelter has been located for more than one hundred of years. it has been one of the most important pb production sites in europe until its closedown in march 2003. ore smelting process generated considerable atmospheric emissions of dust. despite an active environmental strategy, these emissions were still significant in 2002 with up to 17 tonnes of pb, 32 tonnes of zn and 1 tonne of cd. over the years, the generated deposits have led to an important contamination of the surrounding soils. previous studies have shown pollutant transfers to plants, which can induce a risk for human and animal health. the objective of this study was to evaluate the consequences of the smelter closedown on the cd and pb contents of wheat (grain and straw) cultivated in the area. methods. paired topsoil and vegetable samples were taken at harvest time at various distances to the smelter. the sample sites were chosen in order to represent a large range of soil metal contamination. sampling was realised on several wheat harvests between 1997 and 2003. 25 samples were collected before the smelter closedown and 15 after. all ears of about 1 m long of two rows were manually picked and threshed in the lab. similarly, straw was harvested at the same time. total metal contents in soil and wheat samples were quantified. results. a negative correlation between metal concentrations in soil and the distance to the smelter was shown. the wheat grain and straw showed significant cd and pb contents. the straw had higher metal contents than the grain. during the smelter activity, the grain contents were up to 0.8 mg kg–1 dm of cd and 8 mg kg–1 dm of pb. for the straw, maximum contents were 5 mg kg–1 dm of cd and 114 mg kg–1 dm of pb. after the smelter closedown, we observed a very large decrease of pb in the grain (82%) and in the straw (91%). a smaller decrease was observed for cd in grain. despite this improvement, 80% of the studied samples remained non-acceptable for human consumption, according to the european legislation values, due to a high cd content. discussion. results highlighted a difference in metal accumulation in the plant organs as well as a difference in metal uptake. the approach pointed out the importance of atmospheric fallout in the wheat contamination pathways for pb. the smelter closedown has lead to a decrease of the pb content in wheat. it is interesting to relate this finding with the lead blood levels in children living close to the smelter. conclusions. those results have confirmed the importance of dust fallout in the plant contamination pathways. before the closedown, pb measured in the plant was principally originating from the smelter dust emissions. it raised the question of the sanitary risks for humans and animals living in the surrounding area of the smelter. recommendations and perspectives. in the literature, very few articles take the dust deposit as contamination pathways for crops into consideration. however, in highly contaminated sites, this pathway can be very important. thus, it would be worthy studying the uptake of metal contaminants by plants through the foliar system.},
note = {ACL},
keywords = {ER4},
pubstate = {published},
tppubtype = {article}
}
Background. the contamination of soils by heavy metals engenders important environmental and sanitary problems in northern france where a smelter has been located for more than one hundred of years. it has been one of the most important pb production sites in europe until its closedown in march 2003. ore smelting process generated considerable atmospheric emissions of dust. despite an active environmental strategy, these emissions were still significant in 2002 with up to 17 tonnes of pb, 32 tonnes of zn and 1 tonne of cd. over the years, the generated deposits have led to an important contamination of the surrounding soils. previous studies have shown pollutant transfers to plants, which can induce a risk for human and animal health. the objective of this study was to evaluate the consequences of the smelter closedown on the cd and pb contents of wheat (grain and straw) cultivated in the area. methods. paired topsoil and vegetable samples were taken at harvest time at various distances to the smelter. the sample sites were chosen in order to represent a large range of soil metal contamination. sampling was realised on several wheat harvests between 1997 and 2003. 25 samples were collected before the smelter closedown and 15 after. all ears of about 1 m long of two rows were manually picked and threshed in the lab. similarly, straw was harvested at the same time. total metal contents in soil and wheat samples were quantified. results. a negative correlation between metal concentrations in soil and the distance to the smelter was shown. the wheat grain and straw showed significant cd and pb contents. the straw had higher metal contents than the grain. during the smelter activity, the grain contents were up to 0.8 mg kg–1 dm of cd and 8 mg kg–1 dm of pb. for the straw, maximum contents were 5 mg kg–1 dm of cd and 114 mg kg–1 dm of pb. after the smelter closedown, we observed a very large decrease of pb in the grain (82%) and in the straw (91%). a smaller decrease was observed for cd in grain. despite this improvement, 80% of the studied samples remained non-acceptable for human consumption, according to the european legislation values, due to a high cd content. discussion. results highlighted a difference in metal accumulation in the plant organs as well as a difference in metal uptake. the approach pointed out the importance of atmospheric fallout in the wheat contamination pathways for pb. the smelter closedown has lead to a decrease of the pb content in wheat. it is interesting to relate this finding with the lead blood levels in children living close to the smelter. conclusions. those results have confirmed the importance of dust fallout in the plant contamination pathways. before the closedown, pb measured in the plant was principally originating from the smelter dust emissions. it raised the question of the sanitary risks for humans and animals living in the surrounding area of the smelter. recommendations and perspectives. in the literature, very few articles take the dust deposit as contamination pathways for crops into consideration. however, in highly contaminated sites, this pathway can be very important. thus, it would be worthy studying the uptake of metal contaminants by plants through the foliar system. |
2008Article de journal ER4 Auteurs : Gimbert, Frédéric; De-Vaufleury, Annette; Douay, Francis; Coeurdassier, Michaël; Scheifler, Renaud; Badot, Pierre-Marie Long-term responses of snails exposed to cadmium-contaminated soils in a partial life-cycle experiment Dans: Ecotoxicology and Environmental Safety, vol. 70, p. 138-146, 2008, (ACL). Résumé @article{Gimbert2008,
title = {Long-term responses of snails exposed to cadmium-contaminated soils in a partial life-cycle experiment},
author = {Frédéric Gimbert and Annette De-Vaufleury and Francis Douay and Michaël Coeurdassier and Renaud Scheifler and Pierre-Marie Badot},
year = {2008},
date = {2008-01-01},
journal = {Ecotoxicology and Environmental Safety},
volume = {70},
pages = {138-146},
abstract = {Juvenile snails were exposed during their growth period to cd-contaminated field and artificial soils and then transferred to uncontaminated soil to assess the sequels of previous exposure on adult reproduction. growth modelling highlighted growth inhibitions of 5% and 10% after 70 and 84 days of exposure to 20 and 100mgcdkg(-1) in artificial soils, respectively. growth disruption was accompanied by a decrease in the clutch number and a 4-week delay in the egg-laying cycle. although it was also contaminated at 20mgcdkg(-1), the contaminated field soil did not lead to detectable effects in snails, suggesting a lower cd bioavailability confirmed by the bioaccumulation analysis. we demonstrated that the 28-day growth test, as advised by the iso-guideline, may not be sufficient to assess sublethal toxic effects of realistically contaminated soils. for this purpose, a life cycle experimental set-up is proposed, allowing a thorough assessment of toxicity during successive life stages.},
note = {ACL},
keywords = {ER4},
pubstate = {published},
tppubtype = {article}
}
Juvenile snails were exposed during their growth period to cd-contaminated field and artificial soils and then transferred to uncontaminated soil to assess the sequels of previous exposure on adult reproduction. growth modelling highlighted growth inhibitions of 5% and 10% after 70 and 84 days of exposure to 20 and 100mgcdkg(-1) in artificial soils, respectively. growth disruption was accompanied by a decrease in the clutch number and a 4-week delay in the egg-laying cycle. although it was also contaminated at 20mgcdkg(-1), the contaminated field soil did not lead to detectable effects in snails, suggesting a lower cd bioavailability confirmed by the bioaccumulation analysis. we demonstrated that the 28-day growth test, as advised by the iso-guideline, may not be sufficient to assess sublethal toxic effects of realistically contaminated soils. for this purpose, a life cycle experimental set-up is proposed, allowing a thorough assessment of toxicity during successive life stages. |
2008Article de journal ER4 Auteurs : Griffiths, B S; Caul, S; Thompson, J; Hackett, C A; Cortet, Jérôme; Pernin, Céline; Krogh, P H Soil microbial and faunal responses to herbicide tolerant maize andherbicide in two soils Dans: Plant and Soil, vol. 308, p. 93-103, 2008, (ACL). Résumé @article{Griffiths2008,
title = {Soil microbial and faunal responses to herbicide tolerant maize andherbicide in two soils},
author = {B S Griffiths and S Caul and J Thompson and C A Hackett and Jérôme Cortet and Céline Pernin and P H Krogh},
year = {2008},
date = {2008-01-01},
journal = {Plant and Soil},
volume = {308},
pages = {93-103},
abstract = {A glasshouse experiment was set up to compare processes and organisms in two soils planted with genetically modified (gm) herbicide tolerant (ht) maize treated with appropriate herbicides. this was part of a wider project (ecogen) looking at the consequences of gm cropping systems on soil biology using a tiered approach at laboratory, glasshouse and field scales. soil for the experiment was taken from field sites where the same maize cultivars were grown to allow comparison between results under glasshouse and field conditions. the maize cultivars t25 (gm ht glufosinate-ammonium tolerant), orient (non ht near isogenic control for t25) and monumental (a conventional, non ht variety) were grown in contrasting sandy loam and clay loam soils, half were sprayed with the appropriate herbicide as used in the field and soil samples were taken at the five-leaf and flowering plant growth stage. the main effects on all measured parameters were those of soil type and plant growth stage, with four categories of subsequent interaction: (1) there were no effects of herbicide on plant growth or soil microarthropods: (2) the maize cultivar (but not the gm ht trait) had effects on the decomposition of cotton strips and the nematode community; (3) herbicide application in general altered the community level physiological profile of the microbial community and reduced both soil basal respiration and the abundance of protozoa; and (4) the specific application of glufosinate-ammonium to t25 maize altered soil microbial community structure measured by ester linked fatty acids. the results from this glasshouse experiment support the findings from the field that there are effects of herbicide application on the soil microbial and meso-faunal community but that, compared to other standard agricultural practices, the differences are relatively small},
note = {ACL},
keywords = {ER4},
pubstate = {published},
tppubtype = {article}
}
A glasshouse experiment was set up to compare processes and organisms in two soils planted with genetically modified (gm) herbicide tolerant (ht) maize treated with appropriate herbicides. this was part of a wider project (ecogen) looking at the consequences of gm cropping systems on soil biology using a tiered approach at laboratory, glasshouse and field scales. soil for the experiment was taken from field sites where the same maize cultivars were grown to allow comparison between results under glasshouse and field conditions. the maize cultivars t25 (gm ht glufosinate-ammonium tolerant), orient (non ht near isogenic control for t25) and monumental (a conventional, non ht variety) were grown in contrasting sandy loam and clay loam soils, half were sprayed with the appropriate herbicide as used in the field and soil samples were taken at the five-leaf and flowering plant growth stage. the main effects on all measured parameters were those of soil type and plant growth stage, with four categories of subsequent interaction: (1) there were no effects of herbicide on plant growth or soil microarthropods: (2) the maize cultivar (but not the gm ht trait) had effects on the decomposition of cotton strips and the nematode community; (3) herbicide application in general altered the community level physiological profile of the microbial community and reduced both soil basal respiration and the abundance of protozoa; and (4) the specific application of glufosinate-ammonium to t25 maize altered soil microbial community structure measured by ester linked fatty acids. the results from this glasshouse experiment support the findings from the field that there are effects of herbicide application on the soil microbial and meso-faunal community but that, compared to other standard agricultural practices, the differences are relatively small |
2008Article de journal ER4 Auteurs : Lefebvre, Christophe; Vandenbulcke, Franck; Bocquet, Béatrice; Tasiemski, Aurélie; Desmons, Annie; Vierstraete, Mathilde; Salzet, Michel; Cocquerelle, Claude Cathepsin L and cystatin B gene expression discriminates immune cœlomic cells in the leech Theromyzon tessulatum Dans: Developmental and Comparative Immunology, vol. 32, no. 7, p. 795-807, 2008, (ACL). Résumé @article{Lefebvre2008,
title = {Cathepsin L and cystatin B gene expression discriminates immune cœlomic cells in the leech Theromyzon tessulatum},
author = {Christophe Lefebvre and Franck Vandenbulcke and Béatrice Bocquet and Aurélie Tasiemski and Annie Desmons and Mathilde Vierstraete and Michel Salzet and Claude Cocquerelle},
year = {2008},
date = {2008-01-01},
journal = {Developmental and Comparative Immunology},
volume = {32},
number = {7},
pages = {795-807},
abstract = {Previous studies evidenced that cystatin b-like gene is specifically expressed and induced in large circulating coelomic cells following bacterial challenge in the leech theromyzon tessulatum. in order to understand the role of that cysteine proteinase inhibitor during immune response, we investigated the existence of members of cathepsin family. we cloned a cathepsin l-like gene and studied its tissue distribution. immunohistochemical studies using anti-cathepsin l and anti-cystatin b antibodies and ultrastructural results demonstrated the presence of three distinct coelomic cell populations: (1) the chloragocytes, which were initially defined as large coelomocytes, (2) the granular amoebocytes and (3) small coelomic cells. among those cells, while chloragocytes contain cystatin b and cathepsin l, granular amoebocytes contain only cathepsin l and the third cell population contains neither cathepsin nor inhibitor. finally, results evidenced that cathepsin l immunopositive granular amoebocytes are chemoattracted to the site of injury and phagocyte bacteria},
note = {ACL},
keywords = {ER4},
pubstate = {published},
tppubtype = {article}
}
Previous studies evidenced that cystatin b-like gene is specifically expressed and induced in large circulating coelomic cells following bacterial challenge in the leech theromyzon tessulatum. in order to understand the role of that cysteine proteinase inhibitor during immune response, we investigated the existence of members of cathepsin family. we cloned a cathepsin l-like gene and studied its tissue distribution. immunohistochemical studies using anti-cathepsin l and anti-cystatin b antibodies and ultrastructural results demonstrated the presence of three distinct coelomic cell populations: (1) the chloragocytes, which were initially defined as large coelomocytes, (2) the granular amoebocytes and (3) small coelomic cells. among those cells, while chloragocytes contain cystatin b and cathepsin l, granular amoebocytes contain only cathepsin l and the third cell population contains neither cathepsin nor inhibitor. finally, results evidenced that cathepsin l immunopositive granular amoebocytes are chemoattracted to the site of injury and phagocyte bacteria |
2008Conférence ER4 Auteurs : Lemière, Sébastien; Godet, Jean-Philippe; Grumiaux, Fabien; Delplace, Patrick; Demuynck, Sylvain; Vandenbulcke, Franck; Douay, Francis; Leprêtre, Alain Eisenia fetida responses to metal immobilizing agents for contaminated soil remediation: a microcosm evaluation SETAC Europe 18th Annual Meeting. 25-29 May 2008, Krakow (Pologne), 2008, (ACTI). @conference{Lemiere2008,
title = {Eisenia fetida responses to metal immobilizing agents for contaminated soil remediation: a microcosm evaluation},
author = {Sébastien Lemière and Jean-Philippe Godet and Fabien Grumiaux and Patrick Delplace and Sylvain Demuynck and Franck Vandenbulcke and Francis Douay and Alain Leprêtre},
year = {2008},
date = {2008-01-01},
booktitle = {SETAC Europe 18th Annual Meeting. 25-29 May 2008, Krakow (Pologne)},
note = {ACTI},
keywords = {ER4},
pubstate = {published},
tppubtype = {conference}
}
|
2008Conférence ER4 Auteurs : Lemière, Sébastien; Técher, Didier; Delplace, Patrick; Gilliers, C; Demuynck, Sylvain; Vandenbulcke, Franck; Leprêtre, Alain An indoor microcosm study with zebra mussels of metalcontaminated sediment resupension SETAC Europe 18th Annual Meeting, 25-29 May 2008, Krakow (Poland), 2008, (ACTI). @conference{Lemiere2008a,
title = {An indoor microcosm study with zebra mussels of metalcontaminated sediment resupension},
author = {Sébastien Lemière and Didier Técher and Patrick Delplace and C Gilliers and Sylvain Demuynck and Franck Vandenbulcke and Alain Leprêtre},
year = {2008},
date = {2008-01-01},
booktitle = {SETAC Europe 18th Annual Meeting, 25-29 May 2008, Krakow (Poland)},
note = {ACTI},
keywords = {ER4},
pubstate = {published},
tppubtype = {conference}
}
|
2008Article de journal ER4 Auteurs : Munoz, Marcelo; Bachère, Evelyne; Mitta, Guillaume; Vandenbulcke, Franck Analisis de localizacion y distribucion de ARNm de péptidos antimicrobianos expresados en células de camaron por hibridación in situ Dans: Revista Tecnológica ESPOL, vol. 21, no. 1, p. 83-90, 2008, (ACL). Résumé @article{Munoz2008,
title = {Analisis de localizacion y distribucion de ARNm de péptidos antimicrobianos expresados en células de camaron por hibridación in situ},
author = {Marcelo Munoz and Evelyne Bachère and Guillaume Mitta and Franck Vandenbulcke},
year = {2008},
date = {2008-01-01},
journal = {Revista Tecnológica ESPOL},
volume = {21},
number = {1},
pages = {83-90},
abstract = {La presente publicación describe adaptaciones a métodos para la detección y localización de arnmensajeros de penaeidins, péptidos antimicrobianos del camarón penaeus vannamei, utilizandomarcadores fríos y calientes tanto para muestras correspondientes a células citocentrifugadas como paratejidos incluidos en parafina. si se describen los procedimientos y adaptaciones para realizar lostratamientos previos a la hibridación en los cuales se determinan los pasos para desparafinar tejidos,tiempos de post fijación y tratamiento con arnasa para preservar las condiciones de los tejidos para unabuena observación luego de la hibridación. además, se establecen los mejores parámetros para prepararribosondas y se discuten las ventajas de la utilización de este tipo de sondas con respecto a las sondas dedoble cadena de adn. finalmente se determinan los pasos optimizados para realizar una hibridación ydetección de las ribosondas marcadas a la forfatasa alcalina que permitan la mejor definición ysensibilidad en las células objetivo.},
note = {ACL},
keywords = {ER4},
pubstate = {published},
tppubtype = {article}
}
La presente publicación describe adaptaciones a métodos para la detección y localización de arnmensajeros de penaeidins, péptidos antimicrobianos del camarón penaeus vannamei, utilizandomarcadores fríos y calientes tanto para muestras correspondientes a células citocentrifugadas como paratejidos incluidos en parafina. si se describen los procedimientos y adaptaciones para realizar lostratamientos previos a la hibridación en los cuales se determinan los pasos para desparafinar tejidos,tiempos de post fijación y tratamiento con arnasa para preservar las condiciones de los tejidos para unabuena observación luego de la hibridación. además, se establecen los mejores parámetros para prepararribosondas y se discuten las ventajas de la utilización de este tipo de sondas con respecto a las sondas dedoble cadena de adn. finalmente se determinan los pasos optimizados para realizar una hibridación ydetección de las ribosondas marcadas a la forfatasa alcalina que permitan la mejor definición ysensibilidad en las células objetivo. |
2008Article de journal ER4 Auteurs : Pelfrêne, Aurélie; Gassama, Nathalie; Grimaud, Daniel Dissolved Cu(II) speciation in unpolluted soil solutions of a planosolic horizon Dans: Electroanalysis, vol. 20, no. 8, p. 841-850, 2008, (ACL). Résumé @article{Pelfrene2008,
title = {Dissolved Cu(II) speciation in unpolluted soil solutions of a planosolic horizon},
author = {Aurélie Pelfrêne and Nathalie Gassama and Daniel Grimaud},
year = {2008},
date = {2008-01-01},
journal = {Electroanalysis},
volume = {20},
number = {8},
pages = {841-850},
abstract = {Dissolved cu(ii) speciation in unpolluted soil solutions from different horizons was studied using differential pulse anodic stripping voltammetry (dpasv). three sites were selected according to topography and monitored for three years. experiments evidence cu(ii) can be bound by dom (dissolved organic matter) through complexation and/or adsorption reactions. the complexation capacity and the corresponding conditional stability constant were determined for complexation reactions. the adsorbing sites were titrated. a potential binding coefficient was calculated to compare both types of reactions. results show adsorption is less frequent than complexation but exhibits higher binding coefficient.},
note = {ACL},
keywords = {ER4},
pubstate = {published},
tppubtype = {article}
}
Dissolved cu(ii) speciation in unpolluted soil solutions from different horizons was studied using differential pulse anodic stripping voltammetry (dpasv). three sites were selected according to topography and monitored for three years. experiments evidence cu(ii) can be bound by dom (dissolved organic matter) through complexation and/or adsorption reactions. the complexation capacity and the corresponding conditional stability constant were determined for complexation reactions. the adsorbing sites were titrated. a potential binding coefficient was calculated to compare both types of reactions. results show adsorption is less frequent than complexation but exhibits higher binding coefficient. |
2008Article de journal ER4 Auteurs : Pourrut, Bertrand; Perchet, Geoffrey; Silvestre, Jérôme; Cecchi, Marie; Guiresse, Maritxu; Pinelli, Eric Potential role of NADPH-oxidase in early steps of lead-induced oxidative burst in Vicia faba roots Dans: Journal of Plant Physiology, vol. 165, no. 6, p. 571-579, 2008, (ACL). Résumé @article{Pourrut2008,
title = {Potential role of NADPH-oxidase in early steps of lead-induced oxidative burst in Vicia faba roots},
author = {Bertrand Pourrut and Geoffrey Perchet and Jérôme Silvestre and Marie Cecchi and Maritxu Guiresse and Eric Pinelli},
year = {2008},
date = {2008-01-01},
journal = {Journal of Plant Physiology},
volume = {165},
number = {6},
pages = {571-579},
abstract = {The mechanism of oxidative burst induced by lead in vicia faba excised roots was investigated by luminol-dependent chemiluminescence. results showed that lead triggered a rapid and dose-dependent increase in chemiluminescence production. in this study, specific inhibitors of putative reactive oxygen species (ros) sources were used to determine the mechanism of lead-induced ros generation. this generation was sensitive to dephenylene iodonium (dpi), quinacrine and imidazole, some inhibitors of the nadph-oxidase and not inhibited by other putative ros sources inhibitors. data reported in this work clearly demonstrated the pivotal role of nadph-oxidase-like enzyme in early steps of lead-induced oxidative burst. to investigate the respective implication of calmodulin and protein kinase (pk) in leadinduced nadph-oxidase activation, excised roots were treated with the calmodulin inhibitor w7 or with the pk inhibitor staurosporine. the chemiluminescence generation inhibition by these inhibitors illustrated the role of pk in lead-induced nadph-oxidase activation and revealed a calmodulin-dependent step. using the calcium entry blocker la3+ or different concentrations of calcium in the extracellular medium, our data highlighted the implication of ca2+ channel in leadinduced oxidative burst.},
note = {ACL},
keywords = {ER4},
pubstate = {published},
tppubtype = {article}
}
The mechanism of oxidative burst induced by lead in vicia faba excised roots was investigated by luminol-dependent chemiluminescence. results showed that lead triggered a rapid and dose-dependent increase in chemiluminescence production. in this study, specific inhibitors of putative reactive oxygen species (ros) sources were used to determine the mechanism of lead-induced ros generation. this generation was sensitive to dephenylene iodonium (dpi), quinacrine and imidazole, some inhibitors of the nadph-oxidase and not inhibited by other putative ros sources inhibitors. data reported in this work clearly demonstrated the pivotal role of nadph-oxidase-like enzyme in early steps of lead-induced oxidative burst. to investigate the respective implication of calmodulin and protein kinase (pk) in leadinduced nadph-oxidase activation, excised roots were treated with the calmodulin inhibitor w7 or with the pk inhibitor staurosporine. the chemiluminescence generation inhibition by these inhibitors illustrated the role of pk in lead-induced nadph-oxidase activation and revealed a calmodulin-dependent step. using the calcium entry blocker la3+ or different concentrations of calcium in the extracellular medium, our data highlighted the implication of ca2+ channel in leadinduced oxidative burst. |
2008Article de journal ER4 Auteurs : Seignez, Nicolas; Gauthier, Arnaud; Bulteel, David; Damidot, Denis; Potdevin, Jean-Luc Leaching of lead metallurgical slags and pollutant mobility far from equilibrium conditions Dans: Applied Geochemistry, vol. 23, p. 3699-3711, 2008, (ACL). Résumé @article{Seignez2008b,
title = {Leaching of lead metallurgical slags and pollutant mobility far from equilibrium conditions},
author = {Nicolas Seignez and Arnaud Gauthier and David Bulteel and Denis Damidot and Jean-Luc Potdevin},
year = {2008},
date = {2008-01-01},
journal = {Applied Geochemistry},
volume = {23},
pages = {3699-3711},
abstract = {Lead metallurgical slags are partially vitrified materials containing residual amounts of zn, pb, cr, cd and as. these hazardous materials are generally buried on heaps exposed to weathering. in this study, leaching behavior of lead blast furnace slags has been tested using pure water and open flow experiments. it appears that in such far from equilibrium and slightly acidic conditions, the main phase to be altered is the vitreous phase. as for lunar, basaltic and nuclear glasses, alkalis/proton exchanges prevail and lead to the formation of a non-protective altered layer enriched in si, fe and al. the composition of the altered layer is quite constant except for si whose concentration decreases towards the leachate interface. owing to their sizes, micrometric pb droplets are not always totally dissolved at the slag surface. nevertheless, nanometric pb droplets are instantaneously dissolved while a surrounding altered layer is formed. this leads to high pb releases in open flow systems. leachate chemistry and dissolution rates of the vitreous phase are closely comparable to previous leaching tests with basaltic and nuclear glasses in conditions far from equilibrium. moreover, this study confirms that fe is a stable element in such conditions},
note = {ACL},
keywords = {ER4},
pubstate = {published},
tppubtype = {article}
}
Lead metallurgical slags are partially vitrified materials containing residual amounts of zn, pb, cr, cd and as. these hazardous materials are generally buried on heaps exposed to weathering. in this study, leaching behavior of lead blast furnace slags has been tested using pure water and open flow experiments. it appears that in such far from equilibrium and slightly acidic conditions, the main phase to be altered is the vitreous phase. as for lunar, basaltic and nuclear glasses, alkalis/proton exchanges prevail and lead to the formation of a non-protective altered layer enriched in si, fe and al. the composition of the altered layer is quite constant except for si whose concentration decreases towards the leachate interface. owing to their sizes, micrometric pb droplets are not always totally dissolved at the slag surface. nevertheless, nanometric pb droplets are instantaneously dissolved while a surrounding altered layer is formed. this leads to high pb releases in open flow systems. leachate chemistry and dissolution rates of the vitreous phase are closely comparable to previous leaching tests with basaltic and nuclear glasses in conditions far from equilibrium. moreover, this study confirms that fe is a stable element in such conditions |
2008Conférence ER4 Auteurs : Vandenbulcke, Franck Transcriptomic study of Eisenia fetida immune cells French-Hungarian Scientific meeting. 1-2 December 2008, Budapest (Hongrie), 2008, (COM). @conference{Vandenbulcke2008,
title = {Transcriptomic study of Eisenia fetida immune cells},
author = {Franck Vandenbulcke},
year = {2008},
date = {2008-01-01},
booktitle = {French-Hungarian Scientific meeting. 1-2 December 2008, Budapest (Hongrie)},
note = {COM},
keywords = {ER4},
pubstate = {published},
tppubtype = {conference}
}
|
2007Article de journal ER4 Auteurs : Brulle, Franck; Mitta, Guillaume; Leroux, Régine; Lemière, Sébastien; Leprêtre, Alain; Vandenbulcke, Franck The strong induction of metallothionein gene following cadmium exposure transiently affects the expression of many genes in Eisenia fetida: A trade-off mechanism? Dans: Comparative Biochemistry and Physiology C-Toxicology & Pharmacology, vol. 144, no. 4, p. 334-341, 2007, (ACL). Résumé @article{Brulle2007,
title = {The strong induction of metallothionein gene following cadmium exposure transiently affects the expression of many genes in Eisenia fetida: A trade-off mechanism?},
author = {Franck Brulle and Guillaume Mitta and Régine Leroux and Sébastien Lemière and Alain Leprêtre and Franck Vandenbulcke},
year = {2007},
date = {2007-01-01},
journal = {Comparative Biochemistry and Physiology C-Toxicology & Pharmacology},
volume = {144},
number = {4},
pages = {334-341},
abstract = {Metal pollution causes disturbances at various levels of biological organization in most species. important physiological functions could be affected in the exposed individuals and among the main physiological functions, immunity may provide one (or more) effector(s) whose expression can be directly affected by a metal exposure in various macroinvertebrates. protein expressions were studied in order to test them as molecular biomarkers of metal exposure in eisenia fetida. selected effectors were calmodulin, heat shock proteins, superoxide dismutase, catalase, metallothionein, beta-adrenergic receptor kinase, pyruvate carboxylase, transcriptionally controlled tumor protein, protein kinase c, ubiquitin and cyclophilin-a. the level of expression of each gene was analysed in whole organism following exposures to cadmium in soil using real-time pcr. metallothionein, transcriptionally controlled tumor protein and cyclophilin-a expression were also measured following copper exposures in soil because these genes seemed to be sensitive to copper. this work enabled to distinguish metallothionein and cyclophilin-a among the 15 selected effectors. a strong decrease of the number of transcripts was also detected for most effectors soon after the exposure to cadmium suggesting that a trade-off mechanism occurs},
note = {ACL},
keywords = {ER4},
pubstate = {published},
tppubtype = {article}
}
Metal pollution causes disturbances at various levels of biological organization in most species. important physiological functions could be affected in the exposed individuals and among the main physiological functions, immunity may provide one (or more) effector(s) whose expression can be directly affected by a metal exposure in various macroinvertebrates. protein expressions were studied in order to test them as molecular biomarkers of metal exposure in eisenia fetida. selected effectors were calmodulin, heat shock proteins, superoxide dismutase, catalase, metallothionein, beta-adrenergic receptor kinase, pyruvate carboxylase, transcriptionally controlled tumor protein, protein kinase c, ubiquitin and cyclophilin-a. the level of expression of each gene was analysed in whole organism following exposures to cadmium in soil using real-time pcr. metallothionein, transcriptionally controlled tumor protein and cyclophilin-a expression were also measured following copper exposures in soil because these genes seemed to be sensitive to copper. this work enabled to distinguish metallothionein and cyclophilin-a among the 15 selected effectors. a strong decrease of the number of transcripts was also detected for most effectors soon after the exposure to cadmium suggesting that a trade-off mechanism occurs |
2007Article de journal ER4 Auteurs : Gonzalez, Marcelo; Gueguen, Yannick; Destoumieux-Garzón, Delphine; Romestand, Bernard; Fievet, Julie; Pugnière, Martine; Roquet, Françoise; Escoubas, Jean-Michel; Vandenbulcke, Franck; Levy, Ofer; Sauné, Laure; Bulet, Philippe; Bachère, Evelyne Evidence of a bactericidal permeability increasing protein in an invertebrate, the Crassostrea gigas Cg-BPI Dans: Proceedings of the National Academy of Sciences of the United States of America, vol. 104, no. 45, p. 17759-17764, 2007, (ACL). Résumé @article{Gonzalez2007,
title = {Evidence of a bactericidal permeability increasing protein in an invertebrate, the Crassostrea gigas Cg-BPI},
author = {Marcelo Gonzalez and Yannick Gueguen and Delphine Destoumieux-Garzón and Bernard Romestand and Julie Fievet and Martine Pugnière and Françoise Roquet and Jean-Michel Escoubas and Franck Vandenbulcke and Ofer Levy and Laure Sauné and Philippe Bulet and Evelyne Bachère},
year = {2007},
date = {2007-01-01},
journal = {Proceedings of the National Academy of Sciences of the United States of America},
volume = {104},
number = {45},
pages = {17759-17764},
abstract = {A cdna sequence with homologies to members of the lps-binding protein and bactericidal/permeability-increasing protein (bpi) family was identified in the oyster crassostrea gigas. the recombinant protein was found to bind lps, to display bactericidal activity against escherichia coli, and to increase the permeability of the bacterial cytoplasmic membrane. this indicated that it is a bpi rather than an lps-binding protein. by in situ hybridization, the expression of the c. gigas bpi (cg-bpi) was found to be induced in hemocytes after oyster bacterial challenge and to be constitutive in various epithelia of unchallenged oysters. thus, cg-bpi transcripts were detected in the epithelial cells of tissues/organs in contact with the external environment (mantle, gills, digestive tract, digestive gland diverticula, and gonad follicles). therefore, cg-bpi, whose expression profile and biological properties are reminiscent of mammalian bpis, may provide a first line of defense against potential bacterial invasion. to our knowledge, this is the first characterization of a bpi in an invertebrate.},
note = {ACL},
keywords = {ER4},
pubstate = {published},
tppubtype = {article}
}
A cdna sequence with homologies to members of the lps-binding protein and bactericidal/permeability-increasing protein (bpi) family was identified in the oyster crassostrea gigas. the recombinant protein was found to bind lps, to display bactericidal activity against escherichia coli, and to increase the permeability of the bacterial cytoplasmic membrane. this indicated that it is a bpi rather than an lps-binding protein. by in situ hybridization, the expression of the c. gigas bpi (cg-bpi) was found to be induced in hemocytes after oyster bacterial challenge and to be constitutive in various epithelia of unchallenged oysters. thus, cg-bpi transcripts were detected in the epithelial cells of tissues/organs in contact with the external environment (mantle, gills, digestive tract, digestive gland diverticula, and gonad follicles). therefore, cg-bpi, whose expression profile and biological properties are reminiscent of mammalian bpis, may provide a first line of defense against potential bacterial invasion. to our knowledge, this is the first characterization of a bpi in an invertebrate. |
2007Article de journal ER4 Auteurs : Grumiaux, Fabien; Demuynck, Sylvain; Schikorski, David; Lemière, Sébastien; Vandenbulcke, Franck; Leprêtre, Alain Effect of fluidized bed combustion ashes used in metal polluted soil remediation on life history traits of the oligochaeta Eisenia andrei Dans: European Journal of Soil Biology, vol. 43, no. SUPPL. 1, p. S256-S260, 2007, (ACL). @article{Grumiaux2007,
title = {Effect of fluidized bed combustion ashes used in metal polluted soil remediation on life history traits of the oligochaeta Eisenia andrei},
author = {Fabien Grumiaux and Sylvain Demuynck and David Schikorski and Sébastien Lemière and Franck Vandenbulcke and Alain Leprêtre},
year = {2007},
date = {2007-01-01},
journal = {European Journal of Soil Biology},
volume = {43},
number = {SUPPL. 1},
pages = {S256-S260},
note = {ACL},
keywords = {ER4},
pubstate = {published},
tppubtype = {article}
}
|
2007Conférence ER4 Auteurs : Molendi-Coste, Olivier; Laborie, Christine; Breton, Christophe; Mairesse, J; Abbadie, Corinne; Vandenbulcke, Franck; Grumolato, Luca; Aubert, N; Ghzili, Hafida; Gonzalez, B; Vaudry, Hubert; Anouar, Youssef; Vieau, Didier Effets d'une dénutrition maternelle périnatale sur la différenciation neuronale et neuroendocrine dans le modèle de la médullosurrénale: des altérations précoces aux implications possibles dans la programmation de pathologies à l'âge adulte 34ème colloque de la Société de Neuroendocrinologie, 25-27 septembre 2007, Tours, 2007, (ACTN). @conference{Molendi-Coste2007,
title = {Effets d'une dénutrition maternelle périnatale sur la différenciation neuronale et neuroendocrine dans le modèle de la médullosurrénale: des altérations précoces aux implications possibles dans la programmation de pathologies à l'âge adulte},
author = {Olivier Molendi-Coste and Christine Laborie and Christophe Breton and J Mairesse and Corinne Abbadie and Franck Vandenbulcke and Luca Grumolato and N Aubert and Hafida Ghzili and B Gonzalez and Hubert Vaudry and Youssef Anouar and Didier Vieau},
year = {2007},
date = {2007-01-01},
booktitle = {34ème colloque de la Société de Neuroendocrinologie, 25-27 septembre 2007, Tours},
note = {ACTN},
keywords = {ER4},
pubstate = {published},
tppubtype = {conference}
}
|
2007Conférence ER4 Auteurs : Molendi-Coste, Olivier; Vandenbulcke, Franck; Breton, Christophe; Grumolato, Luca; Ghzili, Hafida; Van-Camp, G; Vaudry, Hubert; Anouar, Youssef; Laborie, Christine; Vieau, Didier Effects of maternal perinatal undernutrition on the activity of adrenomedullary cells in 8-month-old rats 8ème Colloque de la Société des Neurosciences. 22-25 mai 2007, Montpellier, 2007, (ACTN). @conference{Molendi-Coste2007a,
title = {Effects of maternal perinatal undernutrition on the activity of adrenomedullary cells in 8-month-old rats},
author = {Olivier Molendi-Coste and Franck Vandenbulcke and Christophe Breton and Luca Grumolato and Hafida Ghzili and G Van-Camp and Hubert Vaudry and Youssef Anouar and Christine Laborie and Didier Vieau},
year = {2007},
date = {2007-01-01},
booktitle = {8ème Colloque de la Société des Neurosciences. 22-25 mai 2007, Montpellier},
note = {ACTN},
keywords = {ER4},
pubstate = {published},
tppubtype = {conference}
}
|
2007Article de journal ER4 Auteurs : Munoz, Marcelo; Bachère, Evelyne; Mitta, Guillaume; Desmons, Annie; Vandenbulcke, Franck Procedimientos Inmunocitoquímicos para Estudiar la Distribución y la Participación en la Defensa de Péptidos Antimicrobianos Dans: Revista Tecnologica ESPOL, vol. 6, p. 79-87, 2007, (ACL). Résumé @article{Munoz2007,
title = {Procedimientos Inmunocitoquímicos para Estudiar la Distribución y la Participación en la Defensa de Péptidos Antimicrobianos},
author = {Marcelo Munoz and Evelyne Bachère and Guillaume Mitta and Annie Desmons and Franck Vandenbulcke},
year = {2007},
date = {2007-01-01},
journal = {Revista Tecnologica ESPOL},
volume = {6},
pages = {79-87},
abstract = {El presente artículo describe adaptaciones y principios básicos para la realización de análisis inmunocitoquímicospara la detección de péptidos antimicrobianos tanto de camarones como de mejillones. estos análisisinmunocitoquímicos, no solamente describen en forma estática la ubicación de estos péptidos, si no que permitirtener una comprensión funcional de los fenómenos ligados a localización y distribución de estos efectores. losanálisis aquí descritos son variados y comienzan con la descripción de la detección los efectores en corteshistológicos provenientes de bloques de parafina y el uso de la microscopía confocal. además, este artículodescribe también protocolos y pone de manifiesto ciertos detalles técnicos de la detección inmune de los efectoresantes o después de la inclusión en la resina asociados a la microscopía electrónica tanto de células como depequeños piezas de tejidos disectados.},
note = {ACL},
keywords = {ER4},
pubstate = {published},
tppubtype = {article}
}
El presente artículo describe adaptaciones y principios básicos para la realización de análisis inmunocitoquímicospara la detección de péptidos antimicrobianos tanto de camarones como de mejillones. estos análisisinmunocitoquímicos, no solamente describen en forma estática la ubicación de estos péptidos, si no que permitirtener una comprensión funcional de los fenómenos ligados a localización y distribución de estos efectores. losanálisis aquí descritos son variados y comienzan con la descripción de la detección los efectores en corteshistológicos provenientes de bloques de parafina y el uso de la microscopía confocal. además, este artículodescribe también protocolos y pone de manifiesto ciertos detalles técnicos de la detección inmune de los efectoresantes o después de la inclusión en la resina asociados a la microscopía electrónica tanto de células como depequeños piezas de tejidos disectados. |
2007Article de journal ER4 Auteurs : Tirapé, Ana; Bacque, Coralie; Brizard, Raphaël; Vandenbulcke, Franck; Boulo, Viviane Expression of immune-related genes in the oyster Crassostrea gigas during ontogenesis Dans: Developmental and Comparative Immunology, vol. 31, no. 9, p. 859-873, 2007, (ACL). @article{Tirape2007,
title = {Expression of immune-related genes in the oyster Crassostrea gigas during ontogenesis},
author = {Ana Tirapé and Coralie Bacque and Raphaël Brizard and Franck Vandenbulcke and Viviane Boulo},
year = {2007},
date = {2007-01-01},
journal = {Developmental and Comparative Immunology},
volume = {31},
number = {9},
pages = {859-873},
note = {ACL},
keywords = {ER4},
pubstate = {published},
tppubtype = {article}
}
|
2006Article de journal ER4 Auteurs : Brulle, Franck; Mitta, Guillaume; Cocquerelle, Claude; Vieau, Didier; Lemière, Sébastien; Leprêtre, Alain; Vandenbulcke, Franck Cloning and real-time PCR testing of 14 potential biomarkers in Eisenia fetida following cadmium exposure Dans: Environmental Science and Technology, vol. 40, no. 8, p. 2844-2850, 2006, (ACL). @article{Brulle2006,
title = {Cloning and real-time PCR testing of 14 potential biomarkers in Eisenia fetida following cadmium exposure},
author = {Franck Brulle and Guillaume Mitta and Claude Cocquerelle and Didier Vieau and Sébastien Lemière and Alain Leprêtre and Franck Vandenbulcke},
year = {2006},
date = {2006-01-01},
journal = {Environmental Science and Technology},
volume = {40},
number = {8},
pages = {2844-2850},
note = {ACL},
keywords = {ER4},
pubstate = {published},
tppubtype = {article}
}
|
2006Conférence ER4 Auteurs : Brulle, Franck; Mitta, Guillaume; Cocquerelle, Claude; Vieau, Didier; Lemière, Sébastien; Leprêtre, Alain; Vandenbulcke, Franck Cloning and real-time PCR testing of 14 potential biomarkers in Eisenia fetida following cadmium exposure 8th International Symposium on Earthworm Ecology, 4-9 September 2006, Krakow (Pologne), 2006, (COM). @conference{Brulle2006a,
title = {Cloning and real-time PCR testing of 14 potential biomarkers in Eisenia fetida following cadmium exposure},
author = {Franck Brulle and Guillaume Mitta and Claude Cocquerelle and Didier Vieau and Sébastien Lemière and Alain Leprêtre and Franck Vandenbulcke},
year = {2006},
date = {2006-01-01},
booktitle = {8th International Symposium on Earthworm Ecology, 4-9 September 2006, Krakow (Pologne)},
note = {COM},
keywords = {ER4},
pubstate = {published},
tppubtype = {conference}
}
|
2006Conférence ER4 Auteurs : Brulle, Franck; Mitta, Guillaume; Cocquerelle, Claude; Vieau, Didier; Lemière, Sébastien; Leprêtre, Alain; Vandenbulcke, Franck Real-time PCR testing of potential biomarkers in soil invertebrates SETAC Europe 16th Annual Meeting, « Controversies and Solutions in Environmental Sciences » 7-11 May 2006, La Haye (Pays-Bas), 2006, (ACTI). @conference{Brulle2006b,
title = {Real-time PCR testing of potential biomarkers in soil invertebrates},
author = {Franck Brulle and Guillaume Mitta and Claude Cocquerelle and Didier Vieau and Sébastien Lemière and Alain Leprêtre and Franck Vandenbulcke},
year = {2006},
date = {2006-01-01},
booktitle = {SETAC Europe 16th Annual Meeting, « Controversies and Solutions in Environmental Sciences » 7-11 May 2006, La Haye (Pays-Bas)},
note = {ACTI},
keywords = {ER4},
pubstate = {published},
tppubtype = {conference}
}
|
2006Conférence ER4 Auteurs : Grumiaux, Fabien; Demuynck, Sylvain; Lemière, Sébastien; Vandenbulcke, Franck; Leprêtre, Alain Effect of fluidized bed combustion ashes used in metal polluted soil remediation on life history traits of the oligochaeta Eisenia andrei 8th International Symposium on Earthworm Ecology, 4-9 September 2006, Krakow (Pologne), 2006, (ACTI). @conference{Grumiaux2006,
title = {Effect of fluidized bed combustion ashes used in metal polluted soil remediation on life history traits of the oligochaeta Eisenia andrei},
author = {Fabien Grumiaux and Sylvain Demuynck and Sébastien Lemière and Franck Vandenbulcke and Alain Leprêtre},
year = {2006},
date = {2006-01-01},
booktitle = {8th International Symposium on Earthworm Ecology, 4-9 September 2006, Krakow (Pologne)},
note = {ACTI},
keywords = {ER4},
pubstate = {published},
tppubtype = {conference}
}
|
2006Conférence ER4 Auteurs : Molendi-Coste, Olivier; Vandenbulcke, Franck; Breton, Christophe; Grumolato, Luca; Ghzili, Hafida; Van-Camp, G; Vaudry, Hubert; Anouar, Youssef; Laborie, Christine; Vieau, Didier Effets d'une dénutrition maternelle périnatale sur la fonctionnalité des cellules chromaffines de la médullosurrénale chez le rat mâle adulte 10ème Journée LARC Neurosciences. 10 novembre 2006, Lille, 2006, (ACTN). @conference{Molendi-Coste2006,
title = {Effets d'une dénutrition maternelle périnatale sur la fonctionnalité des cellules chromaffines de la médullosurrénale chez le rat mâle adulte},
author = {Olivier Molendi-Coste and Franck Vandenbulcke and Christophe Breton and Luca Grumolato and Hafida Ghzili and G Van-Camp and Hubert Vaudry and Youssef Anouar and Christine Laborie and Didier Vieau},
year = {2006},
date = {2006-01-01},
booktitle = {10ème Journée LARC Neurosciences. 10 novembre 2006, Lille},
note = {ACTN},
keywords = {ER4},
pubstate = {published},
tppubtype = {conference}
}
|
2005Conférence ER4 Auteurs : Brulle, Franck; Mitta, Guillaume; Cocquerelle, Claude; Vieau, Didier; Lemière, Sébastien; Leprêtre, Alain; Vandenbulcke, Franck Identification, characterization and validation of pollution biomarkers in soil invertebrates Ecotox 2005. Advances and Trends in Ecotoxicology. 5-7 September 2005, Brno (Czech Republic), 2005, (COM). @conference{Brulle2005,
title = {Identification, characterization and validation of pollution biomarkers in soil invertebrates},
author = {Franck Brulle and Guillaume Mitta and Claude Cocquerelle and Didier Vieau and Sébastien Lemière and Alain Leprêtre and Franck Vandenbulcke},
year = {2005},
date = {2005-01-01},
booktitle = {Ecotox 2005. Advances and Trends in Ecotoxicology. 5-7 September 2005, Brno (Czech Republic)},
note = {COM},
keywords = {ER4},
pubstate = {published},
tppubtype = {conference}
}
|
2004Conférence ER4 Auteurs : Gonzalez, Marcelo; Gueguen, Yannick; Fievet, Julie; Vandenbulcke, Franck; Bachère, Evelyne Molecular characterization of an immune related gene, CgBP/LBP isolated from the oyster Crassostrea gigas Aquaculture Europe 2004: Biotechnologies for quality. 20-23 October 2004, Barcelona (Espagne), 2004, (ACTI). @conference{Gonzalez2004,
title = {Molecular characterization of an immune related gene, CgBP/LBP isolated from the oyster Crassostrea gigas},
author = {Marcelo Gonzalez and Yannick Gueguen and Julie Fievet and Franck Vandenbulcke and Evelyne Bachère},
year = {2004},
date = {2004-01-01},
booktitle = {Aquaculture Europe 2004: Biotechnologies for quality. 20-23 October 2004, Barcelona (Espagne)},
note = {ACTI},
keywords = {ER4},
pubstate = {published},
tppubtype = {conference}
}
|
2004Article de journal ER4 Auteurs : Lefebvre, Christophe; Cocquerelle, Claude; Vandenbulcke, Franck; Hot, David; Huot, Ludovic; Lemoine, Yves; Salzet, Michel Transcriptomic analysis in the leech Theromyzon tessulatum: Involvement of cystatin B in innate immunity Dans: Biochemical Journal, vol. 380, no. 3, p. 617-625, 2004, (ACL). Résumé @article{Lefebvre2004,
title = {Transcriptomic analysis in the leech Theromyzon tessulatum: Involvement of cystatin B in innate immunity},
author = {Christophe Lefebvre and Claude Cocquerelle and Franck Vandenbulcke and David Hot and Ludovic Huot and Yves Lemoine and Michel Salzet},
year = {2004},
date = {2004-01-01},
journal = {Biochemical Journal},
volume = {380},
number = {3},
pages = {617-625},
abstract = {At the present time, there is little information on mechanisms of innate immunity in invertebrate groups other than insects, especially annelids. in the present study, we have performed a transcriptomic study of the immune response in the leech theromyzon tessulatum after bacterial challenge, by a combination of differential display rt (reverse transcriptase)–pcr and cdna microarrays. the results show relevant modulations concerning several known and unknown genes. indeed, threonine deaminase, malate dehydrogenase, cystatin b, polyadenylate-binding protein and α-tubulin-like genes are up-regulated after immunostimulation. we focused on cystatin b (stefin b), which is an inhibitor of cysteine proteinases involved in the vertebrate immune response.we have cloned the full-length cdna and named the t. tessulatum gene as tt-cysb. main structural features of cystatins were identified in the derived amino acid sequence of tt-cysb cdna; namely, a glycine residue in the n-terminus and a consensus sequence of gln-xaa-val-xaa-gly (qxvxg) corresponding to the catalytic site. moreover, tt-cysb is the first cystatin b gene characterized in invertebrates. we have determined by in situ hybridization and immunocytochemistry that tt-cysb is only expressed in large coelomic cells. in addition, this analysis confirmed that tt-cysb is up-regulated after bacterial challenge, and that increased expression occurs only in coelomic cells. these data demonstrate that the innate immune response in the leech involves a cysteine proteinase inhibitor that is not found in ecdysozoan models, such as drosophila melanogaster or caenorhabditis elegans, and so underlines the great need for information about innate immunity mechanisms in different invertebrate groups.},
note = {ACL},
keywords = {ER4},
pubstate = {published},
tppubtype = {article}
}
At the present time, there is little information on mechanisms of innate immunity in invertebrate groups other than insects, especially annelids. in the present study, we have performed a transcriptomic study of the immune response in the leech theromyzon tessulatum after bacterial challenge, by a combination of differential display rt (reverse transcriptase)–pcr and cdna microarrays. the results show relevant modulations concerning several known and unknown genes. indeed, threonine deaminase, malate dehydrogenase, cystatin b, polyadenylate-binding protein and α-tubulin-like genes are up-regulated after immunostimulation. we focused on cystatin b (stefin b), which is an inhibitor of cysteine proteinases involved in the vertebrate immune response.we have cloned the full-length cdna and named the t. tessulatum gene as tt-cysb. main structural features of cystatins were identified in the derived amino acid sequence of tt-cysb cdna; namely, a glycine residue in the n-terminus and a consensus sequence of gln-xaa-val-xaa-gly (qxvxg) corresponding to the catalytic site. moreover, tt-cysb is the first cystatin b gene characterized in invertebrates. we have determined by in situ hybridization and immunocytochemistry that tt-cysb is only expressed in large coelomic cells. in addition, this analysis confirmed that tt-cysb is up-regulated after bacterial challenge, and that increased expression occurs only in coelomic cells. these data demonstrate that the innate immune response in the leech involves a cysteine proteinase inhibitor that is not found in ecdysozoan models, such as drosophila melanogaster or caenorhabditis elegans, and so underlines the great need for information about innate immunity mechanisms in different invertebrate groups. |
2004Article de journal ER4 Auteurs : Munoz, Marcelo; Vandenbulcke, Franck; Garnier, Julien; Gueguen, Yannick; Bulet, Philippe; Saulnier, Denis; Bachère, Evelyne Involvement of penaeidins in defense reactions of the shrimp Litopenaeus stylirostris to a pathogenic vibrio Dans: Cellular and Molecular Life Sciences, vol. 61, no. 7-8, p. 961-972, 2004, (ACL). @article{Munoz2004,
title = {Involvement of penaeidins in defense reactions of the shrimp Litopenaeus stylirostris to a pathogenic vibrio},
author = {Marcelo Munoz and Franck Vandenbulcke and Julien Garnier and Yannick Gueguen and Philippe Bulet and Denis Saulnier and Evelyne Bachère},
year = {2004},
date = {2004-01-01},
journal = {Cellular and Molecular Life Sciences},
volume = {61},
number = {7-8},
pages = {961-972},
note = {ACL},
keywords = {ER4},
pubstate = {published},
tppubtype = {article}
}
|
2004Article de journal ER4 Auteurs : Rivière, Guillaume; Michaud, Annie; Deloffre, Laurence; Vandenbulcke, Franck; Levoye, Angélique; Breton, Christophe; Corvol, Pierre; Salzet, Michel; Vieau, Didier Characterization of the first non-insect invertebrate functional angiotensin-converting enzyme (ACE): Leech TtACE resembles the N-domain of mammalian ACE Dans: Biochemical Journal, vol. 382, no. 2, p. 565-573, 2004, (ACL). Résumé @article{Riviere2004,
title = {Characterization of the first non-insect invertebrate functional angiotensin-converting enzyme (ACE): Leech TtACE resembles the N-domain of mammalian ACE},
author = {Guillaume Rivière and Annie Michaud and Laurence Deloffre and Franck Vandenbulcke and Angélique Levoye and Christophe Breton and Pierre Corvol and Michel Salzet and Didier Vieau},
year = {2004},
date = {2004-01-01},
journal = {Biochemical Journal},
volume = {382},
number = {2},
pages = {565-573},
abstract = {Angiotensin-converting enzyme (ace) is a zinc metallopeptidase that plays a major role in blood homoeostasis and reproduction in mammals. in vertebrates, both transmembrane and soluble ace, containing one or two homologous active sites, have been characterized. so far, several aces from invertebrates have been cloned, but only in insects. they are soluble and display a single active site. using biochemical procedures, an ace-like activity was detected in our model, the leech, theromyzon tessulatum. annelida is the most distant phylum in which an ace activity has been observed. to gain more insight into the leech enzyme, we have developed a pcr approach to characterize its mrna. the approx. 2 kb cdna has been predicted to encode a 616-amino-acid soluble enzyme containing a single active site, named ttace (t. tessulatum ace). surprisingly, its primary sequence shows greater similarity to vertebrates than to invertebrates. stable in vitro expression of ttace in transfected chinese-hamster ovary cells revealed that the leech enzyme is a functional metalloprotease. as in mammals, this 79 kda glycosylated enzyme functions as a dipeptidyl carboxypeptidase capable of hydrolysing angiotensin i to angiotensin ii. however, a weak chloride inhibitory effect and acetylated n-acetyl-sdkp (ac sdackp) hydrolysis reveal that ttace activity resembles that of the n-domain of mammalian ace. in situ hybridization shows that its cellular distribution is restricted to epithelial midgut cells. although the precise roles and endogenous substrates of ttace remain to be identified, characterization of this ancestral peptidase will help to clarify its physiological roles in non-insect invertebrate species.},
note = {ACL},
keywords = {ER4},
pubstate = {published},
tppubtype = {article}
}
Angiotensin-converting enzyme (ace) is a zinc metallopeptidase that plays a major role in blood homoeostasis and reproduction in mammals. in vertebrates, both transmembrane and soluble ace, containing one or two homologous active sites, have been characterized. so far, several aces from invertebrates have been cloned, but only in insects. they are soluble and display a single active site. using biochemical procedures, an ace-like activity was detected in our model, the leech, theromyzon tessulatum. annelida is the most distant phylum in which an ace activity has been observed. to gain more insight into the leech enzyme, we have developed a pcr approach to characterize its mrna. the approx. 2 kb cdna has been predicted to encode a 616-amino-acid soluble enzyme containing a single active site, named ttace (t. tessulatum ace). surprisingly, its primary sequence shows greater similarity to vertebrates than to invertebrates. stable in vitro expression of ttace in transfected chinese-hamster ovary cells revealed that the leech enzyme is a functional metalloprotease. as in mammals, this 79 kda glycosylated enzyme functions as a dipeptidyl carboxypeptidase capable of hydrolysing angiotensin i to angiotensin ii. however, a weak chloride inhibitory effect and acetylated n-acetyl-sdkp (ac sdackp) hydrolysis reveal that ttace activity resembles that of the n-domain of mammalian ace. in situ hybridization shows that its cellular distribution is restricted to epithelial midgut cells. although the precise roles and endogenous substrates of ttace remain to be identified, characterization of this ancestral peptidase will help to clarify its physiological roles in non-insect invertebrate species. |
2004Conférence ER4 Auteurs : Sautière, Pierre-Eric; Vergote, David; Vandenbulcke, Franck; Mitta, Guillaume; Vizioli, Jacopo; Macagno, Eduardo-R.; Salzet, Michel Leech central nervous system response following septic challenge: a proteomic approach First international meeting on Evolution, Development and neurobiology of the leech. 29 septembre-3 octobre 2004, Villeneuve d’Ascq, 2004, (ACTI). @conference{Sautiere2004,
title = {Leech central nervous system response following septic challenge: a proteomic approach},
author = {Pierre-Eric Sautière and David Vergote and Franck Vandenbulcke and Guillaume Mitta and Jacopo Vizioli and Eduardo-R. Macagno and Michel Salzet},
year = {2004},
date = {2004-01-01},
booktitle = {First international meeting on Evolution, Development and neurobiology of the leech. 29 septembre-3 octobre 2004, Villeneuve d’Ascq},
note = {ACTI},
keywords = {ER4},
pubstate = {published},
tppubtype = {conference}
}
|
2004Article de journal ER4 Auteurs : Tasiemski, Aurélie; Vandenbulcke, Franck; Mitta, Guillaume; Lemoine, Jérôme; Lefebvre, Christophe; Sautière, Pierre-Eric; Salzet, Michel Molecular characterization of two novel antibacterial peptides inducible upon bacterial challenge in an annelid, the leech Theromyzon tessulatum Dans: Journal of Biological Chemistry, vol. 279, no. 30, p. 30973-30982, 2004, (ACL). @article{Tasiemski2004,
title = {Molecular characterization of two novel antibacterial peptides inducible upon bacterial challenge in an annelid, the leech Theromyzon tessulatum},
author = {Aurélie Tasiemski and Franck Vandenbulcke and Guillaume Mitta and Jérôme Lemoine and Christophe Lefebvre and Pierre-Eric Sautière and Michel Salzet},
year = {2004},
date = {2004-01-01},
journal = {Journal of Biological Chemistry},
volume = {279},
number = {30},
pages = {30973-30982},
note = {ACL},
keywords = {ER4},
pubstate = {published},
tppubtype = {article}
}
|
2004Conférence ER4 Auteurs : Tasiemski, Aurélie; Vandenbulcke, Franck; Mitta, Guillaume; Lemoine, Jérôme; Lefebvre, Christophe; Sautière, Pierre-Eric; Salzet, Michel Molecular characterization of two novel antibacterial peptides inducible upon bacterial challenge in an annelid, the leech Theromyzon tessulatum First international meeting on Evolution, Development and neurobiology of the leech. 29 septembre-3 octobre 2004, Villeneuve d’Ascq, France, 2004, (ACTI). @conference{Tasiemski2004a,
title = {Molecular characterization of two novel antibacterial peptides inducible upon bacterial challenge in an annelid, the leech Theromyzon tessulatum},
author = {Aurélie Tasiemski and Franck Vandenbulcke and Guillaume Mitta and Jérôme Lemoine and Christophe Lefebvre and Pierre-Eric Sautière and Michel Salzet},
year = {2004},
date = {2004-01-01},
booktitle = {First international meeting on Evolution, Development and neurobiology of the leech. 29 septembre-3 octobre 2004, Villeneuve d’Ascq, France},
note = {ACTI},
keywords = {ER4},
pubstate = {published},
tppubtype = {conference}
}
|
2004Conférence ER4 Auteurs : Vandenbulcke, Franck Necessity to explore innate immunity in diverse models: the leech Theromyzon tessulatum XIX International Congress of Zoology. 23-27 August 2004, Beijing (China), 2004, (INV). @conference{Vandenbulcke2004,
title = {Necessity to explore innate immunity in diverse models: the leech Theromyzon tessulatum},
author = {Franck Vandenbulcke},
year = {2004},
date = {2004-01-01},
booktitle = {XIX International Congress of Zoology. 23-27 August 2004, Beijing (China)},
note = {INV},
keywords = {ER4},
pubstate = {published},
tppubtype = {conference}
}
|
2004Article de journal ER4 Auteurs : Vergote, David; Sautière, Pierre-Eric; Vandenbulcke, Franck; Vieau, Didier; Mitta, Guillaume; Macagno, Eduardo-R.; Salzet, Michel Up-regulation of neurohemerythrin expression in the central nervous system of the medicinal leech, Hirudo medicinalis, following septic injury Dans: Journal of Biological Chemistry, vol. 279, no. 42, p. 43828-43837, 2004, (ACL). @article{Vergote2004,
title = {Up-regulation of neurohemerythrin expression in the central nervous system of the medicinal leech, Hirudo medicinalis, following septic injury},
author = {David Vergote and Pierre-Eric Sautière and Franck Vandenbulcke and Didier Vieau and Guillaume Mitta and Eduardo-R. Macagno and Michel Salzet},
year = {2004},
date = {2004-01-01},
journal = {Journal of Biological Chemistry},
volume = {279},
number = {42},
pages = {43828-43837},
note = {ACL},
keywords = {ER4},
pubstate = {published},
tppubtype = {article}
}
|
2003Conférence ER4 Auteurs : Bachère, Evelyne; Destoumieux-Garzón, Delphine; Munoz, Marcelo; Vandenbulcke, Franck; Bulet, Philippe Penaeidins, a family of antimicrobial peptides ubiquitous in Crustacea: involvement in shrimp immune response to infections 4th International Gordon Research Conference on Antimicrobial Peptides, 27 April-2 May 2003, Barga (Italie), 2003, (ACTI). @conference{Bachere2003,
title = {Penaeidins, a family of antimicrobial peptides ubiquitous in Crustacea: involvement in shrimp immune response to infections},
author = {Evelyne Bachère and Delphine Destoumieux-Garzón and Marcelo Munoz and Franck Vandenbulcke and Philippe Bulet},
year = {2003},
date = {2003-01-01},
booktitle = {4th International Gordon Research Conference on Antimicrobial Peptides, 27 April-2 May 2003, Barga (Italie)},
note = {ACTI},
keywords = {ER4},
pubstate = {published},
tppubtype = {conference}
}
|
2003Conférence ER4 Auteurs : Bachère, Evelyne; Munoz, Marcelo; Vandenbulcke, Franck; Bulet, Philippe; Garnier, Julien; Fievet, Julie; Dietrich, Jacques; Romestand, Bernard; Gueguen, Yannick Penaeidines, une famille de peptides antimicrobiens: implication dans la réponse immunitaire Réunion Immunologie des Invertébrés. 17-19 novembre 2003, Banyuls, 2003, (ACTN). @conference{Bachere2003a,
title = {Penaeidines, une famille de peptides antimicrobiens: implication dans la réponse immunitaire},
author = {Evelyne Bachère and Marcelo Munoz and Franck Vandenbulcke and Philippe Bulet and Julien Garnier and Julie Fievet and Jacques Dietrich and Bernard Romestand and Yannick Gueguen},
year = {2003},
date = {2003-01-01},
booktitle = {Réunion Immunologie des Invertébrés. 17-19 novembre 2003, Banyuls},
note = {ACTN},
keywords = {ER4},
pubstate = {published},
tppubtype = {conference}
}
|
2003Conférence ER4 Auteurs : Gonzalez, Marcelo; Gueguen, Yannick; Escoubas, Jean-Michel; Dietrich, Jacques; Fievet, Julie; Vandenbulcke, Franck; Bachère, Evelyne Identification de la première protéine de type LBP/BPI chez un invertébré, l’huitre creuse Crassotrea gigas: étude de l’expression du gène et caractérisation préliminaire de la protéine recombinante Réunion Immunologie des Invertébrés. 17-19 novembre 2003, Banyuls, 2003, (ACTN). @conference{Gonzalez2003,
title = {Identification de la première protéine de type LBP/BPI chez un invertébré, l’huitre creuse Crassotrea gigas: étude de l’expression du gène et caractérisation préliminaire de la protéine recombinante},
author = {Marcelo Gonzalez and Yannick Gueguen and Jean-Michel Escoubas and Jacques Dietrich and Julie Fievet and Franck Vandenbulcke and Evelyne Bachère},
year = {2003},
date = {2003-01-01},
booktitle = {Réunion Immunologie des Invertébrés. 17-19 novembre 2003, Banyuls},
note = {ACTN},
keywords = {ER4},
pubstate = {published},
tppubtype = {conference}
}
|
2003Conférence ER4 Auteurs : Lefebvre, Christophe; Cocquerelle, Claude; Vandenbulcke, Franck; Hot, David; Huot, Ludovic; Lemoine, Yves; Salzet, Michel Implication de la cystatine B et des cathepsines dans l'immunité innée chez la sangsue Theromyzon tessulatum Réunion Immunologie des Invertébrés. 17-19 novembre 2003, Banyuls, 2003, (COM). @conference{Lefebvre2003,
title = {Implication de la cystatine B et des cathepsines dans l'immunité innée chez la sangsue Theromyzon tessulatum},
author = {Christophe Lefebvre and Claude Cocquerelle and Franck Vandenbulcke and David Hot and Ludovic Huot and Yves Lemoine and Michel Salzet},
year = {2003},
date = {2003-01-01},
booktitle = {Réunion Immunologie des Invertébrés. 17-19 novembre 2003, Banyuls},
note = {COM},
keywords = {ER4},
pubstate = {published},
tppubtype = {conference}
}
|
2003Article de journal ER4 Auteurs : Munoz, Marcelo; Vandenbulcke, Franck; Gueguen, Yannick; Bachère, Evelyne Expression of penaeidin antimicrobial peptides in early larval stages of the shrimp Penaeus vannamei Dans: Developmental and Comparative Immunology, vol. 27, no. 4, p. 283-289, 2003, (ACL). @article{Munoz2003,
title = {Expression of penaeidin antimicrobial peptides in early larval stages of the shrimp Penaeus vannamei},
author = {Marcelo Munoz and Franck Vandenbulcke and Yannick Gueguen and Evelyne Bachère},
year = {2003},
date = {2003-01-01},
journal = {Developmental and Comparative Immunology},
volume = {27},
number = {4},
pages = {283-289},
note = {ACL},
keywords = {ER4},
pubstate = {published},
tppubtype = {article}
}
|
2003Conférence ER4 Auteurs : Roch, Philippe; Mitta, Guillaume; Vandenbulcke, Franck; Salzet, Michel; Aumelas, André; Yang, Yin-Shan; Chavanieu, Alain; Calas, Bernard Originality of the Mytilus (Bivalve Mollusc) antibacterial peptides: structurally related to insects but involved as in mammals The new panorama of animal evolution. Proceedings of the XVIII International Congress of Zoology, September 2000, Athens (Greece), Pensoft Publishers. Sofia-Moscow, 2003, (ACTI). @conference{Roch2003,
title = {Originality of the Mytilus (Bivalve Mollusc) antibacterial peptides: structurally related to insects but involved as in mammals},
author = {Philippe Roch and Guillaume Mitta and Franck Vandenbulcke and Michel Salzet and André Aumelas and Yin-Shan Yang and Alain Chavanieu and Bernard Calas},
year = {2003},
date = {2003-01-01},
booktitle = {The new panorama of animal evolution. Proceedings of the XVIII International Congress of Zoology, September 2000, Athens (Greece)},
pages = {177-184},
publisher = {Pensoft Publishers. Sofia-Moscow},
note = {ACTI},
keywords = {ER4},
pubstate = {published},
tppubtype = {conference}
}
|
