Publications

@conference{Pottiez2015,

title = {Intégration de méthodes de lutte alternative dans des systèmes de culture pour réduire de 50% l’utilisation des produits conventionnels de protection des plantes},

author = {B Pottiez and S Allexandre and E Craye and F Couturier and G Billaut and K Petit and J Bruyere and V Delannoy and Brice Louvel and Francis Douay and S Oste and D Werbrouck and Ali Siah and Patrice Halama},

year  = {2015},

date = {2015-01-01},

booktitle = {5th International Conference on Alternative Methods of Crop Protection, 11-13 mars 2015, Lille},

note = {ACTI},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Pourrut2015a,

title = {Sustainable management of contaminated sites in Northern France},

author = {Bertrand Pourrut},

year  = {2015},

date = {2015-01-01},

booktitle = {Conférence Professeur Invité de l’Université de Tulsa, 30 October 2015. Tulsa University (USA)},

note = {INV},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Goulas2015,

title = {Analytical method for determining polycyclic aromatic hydrocarbon pollutants using ultra-fast liquid chromatography with fluorescence detection and the recent column packed with the new 5 μm Kinetex-C18 core-shell particles},

author = {Anaïs Goulas and Brice Louvel and Christophe Waterlot},

year  = {2015},

date = {2015-01-01},

journal = {Canadian Journal of Chemistry},

volume = {93},

number = {5},

pages = {564-571},

abstract = {Nous avons optimisé une méthode de chromatographie liquide à ultra haute performance à détection par fluorescence pour réaliser le dosage de 15 hydrocarbures aromatiques polycycliques (hap) en employant la nouvelle colonne kinetex-c18 (250 mm × 4,6 mm). nous avons utilisé cette colonne récemment commercialisée, contenant un nouveau type de particules poreuses de type core-shell dont la taille moyenne est de 5 μm et fonctionnant à très basse pression, afin de séparer divers composés par chromatographie liquide. après optimisation de la méthode analytique, la séparation des 15 hap dans des échantillons d’eau du robinet dopés a été réalisée, sans coélution de produits, en 21,5 min à 16 °c, dans une phase mobile qui consistait en un mélange aqueux d’acétonitrile, en conditions de gradient de concentrations, à très faible débit (0,7 à 1,0 ml min−1) et à faible pression (870 à 1 590 psi = 60 à 110 bar); toutes ces conditions présentant un intérêt sur le plan économique. pour chacun des composés, nous avons effectué la synchronisation entre les variations de longueur d’onde dans le temps et le temps d’élution dans le but de prévenir une dérive de la ligne de base. nous avons mené la validation de l’ensemble de la procédure expérimentale en tenant compte des paramètres suivants : courbe de calibration, linéarité, limites de détection et de quantification, exactitude, sensibilité, précision et répétabilité du temps de rétention de chaque hap. la méthode analytique proposée présentait une bonne linéarité dans la gamme de concentrations entre 0,025 et 10 μg l−1, où le coefficient de corrélation était supérieur à 0,9980. la répétabilité (écart-type relatif en pour cent},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Grumiaux2015,

title = {Earthworm populations of highly metal-contaminated soils restored by fly ash-aided phytostabilisation},

author = {Fabien Grumiaux and Sylvain Demuynck and Céline Pernin and Alain Leprêtre},

year  = {2015},

date = {2015-01-01},

journal = {Ecotoxicology and Environmental Safety},

volume = {113},

pages = {183-190},

abstract = {Highly metal contaminated soils found in the north of france are the result of intense industrial past. these soils are now unfit for the cultivation of agricultural products for human consumption. solutions have to be found to improve the quality of these soils, and especially to reduce the availability of trace elements (tes). phytostabilisation and ash-aided phytostabilisation applied since 2000 to an experimental site located near a former metallurgical site (metaleurop-nord) was shown previously as efficacious in reducing tes mobility in soils. the aim of the study was to check whether this ten years trial had influenced earthworm communities. this experimental site was compared to plots located in the surroundings and differing by the use of soils. main results are that: (1) whatever the use of soils, earthworm communities are composed of few species with moderate abundance in comparison with communities found in similar habitats outside the tes-contaminated area, (2) the highest abundance and specific richness (4-5 species) were observed in afforested plots with various tree species, (3) ash amendments in afforested plots did not increase the species richness and modified the communities favoring anecic worms but disfavoring epigeic ones. these findings raised the questions of when and how to perform the addition of ashes firstly, to avoid negative effects on soil fauna and secondly, to keep positive effects on metal immobilization. (c) 2014 elsevier inc. all rights reserved.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Pourrut2015b,

title = {Recommendations for increasing alkaline comet assay reliability in plants},

author = {Bertrand Pourrut and Eric Pinelli and Vanessa Celiz-Mendiola and Jérôme Silvestre and Francis Douay},

year  = {2015},

date = {2015-01-01},

journal = {Mutagenesis},

volume = {30},

number = {1},

pages = {37-43},

abstract = {In plants, an increasing interest for the comet assay was shown in the last decade. this versatile technique appears to be promising to detect the genotoxic effect of pollutants and to monitor the environment. however, the lack of a standardised protocol and the low throughput of the assay limit its use in plants. the aims of this paper are to identify key factors affecting comet assay performance and to improve its reliability and reproducibility. we examined the effect of varying several parameters on four different plant species: broad bean (vicia faba), white clover (trifolium repens), english ryegrass (lolium perenne) and miscanthus (miscanthus x giganteus). the influence of both internal (different nucleus isolation methods, presence or absence of filtration and lysis steps) and external (room temperature, light intensity) parameters were evaluated. results clearly indicate that short chopping is more efficient to isolate nuclei than the standard slicing method. filtration and lysis steps were shown to be unnecessary and thus should be skipped. data also demonstrate that high room temperatures and light could induce dna damage in isolated nuclei. calibration tests with h2o2 or ethyl methanesulfonate revealed that a special attention should be paid to plant growing stage, leaf position and exposure duration.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Pourrut2015bb,

title = {Development of an automated scoring system for plant comet assay},

author = {Bertrand Pourrut and Irinah Ratsizafy and Charles Homsy and Julien Dubus and Karim Al-Souki and Francis Douay and Michel Soussaline and Françoise Soussaline},

year  = {2015},

date = {2015-01-01},

booktitle = {ICAW 2015 - 11th International Comet Assay Workshop, 1- 4 September 2015, Antwerpen (Belgique)},

abstract = {In plants, an increasing interest for the comet assay was shown in the last decade and this versatile technique appears to be promising to detect the genotoxic effect of pollutants and to monitor the environment. however, its use in plant studies was rather limited compared to animal studies because of (i) the difficulty to isolate intact nuclei compared to animal systems and the lack of a standardized protocol, (ii) the low throughput of current nucleus extraction, and (iii) the lack of a high throughput comet assay scoring method. in order to deal with these issues, we recently identified the key steps of the comet assay on plant models and proposed an optimized protocol to increase its reliability (pourrut et al.; 2015). at the same time, in the frame of the french-norwegian project compack (2014-2017), we have worked on: -the development a new nucleus extraction technique compatible with the high-throughput comet assay scoring methods; -the automation of the scoring method based on the automated scoring system pathfinder™, developed by imstar. details and results from the preliminary experiments will be presented and discussed. major issues have been: -scoring system: specific adaptation of the automated scoring system pathfinder™ is crucial as it was initially set up for human/animal cells; -background: optimization of the protocol to reduce the presence of debris and increase background quality; -nucleus density: increase the density of nuclei is of importance to increase scoring reliability (sharma et al., 2012). in conclusion, increasing plant nucleus extraction yield and automated scoring of nuclei do represent big challenges. however, our promising preliminary results open up the perspective of an automated high-throughput scoring of plant nuclei. acknowledgements authors are grateful to the french-norwegian foundation ffs/fns, the french ministry of higher education and research, and bpi france for the financial support of the compack project},

note = {ACTI},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Hoareau2015,

title = {Burial diagenesis of the Eocene Sobrarbe delta (Ainsa Basin, Spain) inferred from dolomitic concretions},

author = {Guilhem Hoareau and Francis Odonne and Daniel Garcia and Elie-Jean Debroas and Christophe Monnin and Michel Dubois and Jean-Luc Potdevin},

year  = {2015},

date = {2015-01-01},

journal = {Journal of Sedimentary Research},

volume = {85},

number = {9},

pages = {1037-1057},

abstract = {Little attention has been focused on the burial diagenesis of deltas deposited on active foreland-basin margins, where tectonics is likely to strongly impact fluid–rock interactions. a petrographic, geochemical, and microthermometric study of several fractured dolomite concretions and enclosing prodelta marls provides insights into the evolution of burial diagenesis in the eocene sobrarbe deltaic complex (ainsa basin, spain), and more generally, on the paleohydrology of the south pyrenean foreland basin. shallow burial diagenesis was controlled by microbial activity in marine-derived porewaters. microbial sulfate reduction was first responsible for the formation of pyrite and early calcite, followed by the growth of dolomite concretions during methanogenesis. subsequent diagenesis was limited to temperatures and depth of less than approximately 75°c and 2 km, respectively. diagenesis was recorded in porous bioturbation traces and septarian fractures found inside dolomite concretions, as well as in tectonic shear fractures. neomorphic tabular barite, found only in the bioturbation traces, is interpreted to have formed early in marine-derived porewaters. septarian fractures were then filled by fe-rich calcite and centimeter-size celestine. stable isotopes indicate that calcite probably formed in meteoric-derived waters coming from the overlying fluvial delta plain. the sulfur isotope composition of celestine is compatible with precipitation in waters of mixed parentage, but the exact origin of dissolved sulfate remains poorly constrained. in tectonic fractures, celestine precipitated coevally with calcite displaying evidence of strong fluid–rock interaction. dissolved sulfate may have migrated to the fractures during active tectonics from the late eocene to the oligocene. the paragenesis and the proposed paleohydrologic model are similar to those previously described for other deltaic systems deposited in active foreland basins, including the south pyrenean foreland basin. these features point to common diagenetic processes in syntectonic foreland-basin deltas, involving both meteoric and marine fluid sources. similar to passive margin settings, early diagenesis appears to be controlled mainly by relative variations of sea level, whereas during further burial, the development of permeable tectonic fractures is likely to facilitate the influx of basinal or continental waters into fine slope deposits, impacting the diagenetic record. these results emphasize the importance of fracture development in the fluid-flow regime of syntectonic foreland-basin deltas. they demonstrate the necessity to take this parameter into account in fluid-flow modeling of foreland-basin margins.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Qasim2015,

title = {Effect of nitrate and ammonium fertilization on Zn, Pb, and Cd phytostabilization by Populus euramericana Dorskamp in contaminated technosol},

author = {Bashar Qasim and Mikael Motelica-Heino and Sylvain Bourgerie and Arnaud Gauthier and Domenico Morabito},

year  = {2015},

date = {2015-01-01},

journal = {Environmental Science and Pollution Research},

volume = {22},

number = {23},

pages = {18759-18771},

abstract = {This study aimed at assessing the effect of nitrogen addition under two forms, nitrate and ammonium, on the stabilization of zn, pb, and cd by populus euramericana dorskamp grown in contaminated soils for 35 days under controlled conditions. temporal changes in the soil pore water (spw) were monitored for ph, dissolved organic carbon (doc), and total dissolved concentrations of metals in the soils rhizosphere. rhizospheric spw ph decreased gradually with nh4 + addition and increased with no3 − addition up to one unit, while it slightly decreased initially then increased for the untreated control soil doc increased with time up to six times, the highest increase occurring with nh4 + fertilization. an increase in the metal concentrations in the rhizospheric spw was observed for nh4 + addition associated with the lowest rhizospheric spw ph, whereas the opposite was observed for the control soil and no3 − fertilization. fertilization did not affect plant shoots or roots biomass development compared to the untreated control (without n addition). metals were mostly accumulated in the rhizosphere and n fertilization increased the accumulation for zn and pb while cd accumulation was enhanced for nh4 + addition. collectively, our results suggest metal stabilization by p. euramericana dorskamp rhizosphere with nitrogen fertilization and are potential for phytostabilization of contaminated technosol},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Homa2015,

title = {Dermal exposure of Eisenia andrei earthworms: Effects of heavy metals on metallothionein and phytochelatin synthase gene expressions in coelomocytes},

author = {Joanna Homa and Agnieszka Rorat and Jerzy Kruk and Claude Cocquerelle and Barbara Plytycz and Franck Vandenbulcke},

year  = {2015},

date = {2015-01-01},

journal = {Environmental Toxicology and Chemistry},

volume = {34},

number = {6},

pages = {1397-1404},

abstract = {Parameters such as total number of coelomocytes, riboflavin content in coelomocytes, expression of genes implied in metal homeostasis, and detoxification mechanisms can be used as biomarkers to assess the impact of metals on annelids. defense biomarkers (detoxification gene expressions and coelomocyte parameters) were investigated in the ecotoxicologically important species eisenia andrei following in vivo exposure to 5 different metals (zinc, copper, nickel, lead, and cadmium) at known concentrations. coelomocyte numbers and riboflavin content were not affected by metallic exposure, but metal-specific gene expression variations were evidenced},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Qasim2015a,

title = {Potentially toxic element phytoavailability assessment in Technosols from former smelting and mining areas},

author = {Bashar Qasim and Mikael Motelica-Heino and Emmanuel Joussein and Maryline Soubrand and Arnaud Gauthier},

year  = {2015},

date = {2015-01-01},

journal = {Environmental Science and Pollution Research},

volume = {22},

pages = {5961-5974},

abstract = {This study reports the chemical bioavailability of several potentially toxic elements (zn, pb, cd, as, and sb) in contaminated technosols from two former smelting and mining areas. though these elements have long been recognized as potentially harmful elements, understanding of their toxicity and environmental behavior in technosols developed on former mining and smelting sites are more limited, particularly for as and sb. surface soils were sampled from metallophyte grassland contaminated with zn, pb, and cd located at mortagne-du-nord (north france) and from a former mining settling basin contaminated with as, pb, and sb located at la petite faye (limoges, france). various selective single extraction procedures (cacl2, nano3, nh4no3, dtpa, and edta) were used together with germination tests with dwarf beans whose shoots were analyzed for their potentially toxic element concentrations after 21 days of growth. the extraction capacity of the potentially toxic elements followed the order edta > dtpa > nh4no3 > cacl2 > nano3 for both studied areas. pearson's correlation coefficient analysis between the concentrations of potentially toxic elements accumulated in bean primary leaves or their mineral mass with their extractable concentrations showed a positive significant correlation with dilute cacl2 and nitrate solutions extraction procedures. in contrast, for all studied elements, except pb, the complexing and chelating extractants (edta and dtpa) exhibited poor correlation with the dwarf bean leaves concentrations. moreover, results showed that the 0.01 m cacl2 extraction procedure was the most suitable and provided the most useful indications of metal phytoavailability for studied elements},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Hullebusch2015,

title = {Bio-alteration of metallurgical wastes by Pseudomonas aeruginosa in a semi flow-through reactor},

author = {Eric-D. van-Hullebusch and Nang-Htay Yin and Nicolas Seignez and Jérôme Labanowski and Arnaud Gauthier and Piet-N.L. Lens and Caroline Avril and Yann Sivry},

year  = {2015},

date = {2015-01-01},

journal = {Journal of Environmental Management},

volume = {147},

pages = {297-305},

abstract = {Metallurgical activities can generate a huge amount of partially vitrified waste products which are either landfilled or recycled. lead blast furnace (lbf) slags are often disposed of in the vicinity of metallurgical plants, and are prone to weathering, releasing potentially toxic chemical components into the local environment. to simulate natural weathering in a slag heap, bioweathering of these lbf slags was studied in the presence of a pure heterotrophic bacterial strain (pseudomonas aeruginosa) and in a semi-flow through reactor with intermittent leachate renewal. the evolution of water chemistry, slag composition and texture were monitored during the experiments. the cumulative bulk release of dissolved fe, si, ca and mg doubled in the presence of bacteria, probably due to the release of soluble complexing organic molecules (e.g. siderophores). in addition, bacterial biomass served as the bioadsorbent for pb, fe and zn as 70–80% of pb and fe, 40–60% of zn released are attached to and immobilized by the bacterial biomass.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Ramadan2015,

title = {Instabilités et évolutions des cavités souterraines de la Métropole lilloise : de l’observation des phénomènes à des applications en laboratoire},

author = {Ghanem Ramadan and Michel Dubois and Arnaud Gauthier and G Berrehouc and Gaetan Cheppe},

year  = {2015},

date = {2015-01-01},

booktitle = {Journée des Doctorants IRePSE, 08 juillet 2015, CERLA - Cité Scientifique - Université Lille 1, Villeneuve d'Ascq},

note = {ACTN},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Jacques2015,

title = {Lipopeptides: an emerging family of biopesticides - Les lipopeptides: une famille emergente de biopesticides Projet interreg phytobio 2010-2013},

author = {Philippe Jacques and François Krier and François Coutte and Sébastien Lemière and Monica Höfte and Marc Ongena and Essaid-Ait Barka and Stephan Dorey and Cédric Jacquard and Philippe Reignault and Béatrice Randoux and Lieven Delanote and Nathalie Cap},

year  = {2015},

date = {2015-01-01},

booktitle = {5th International Conference on Alternative Methods of Crop Protection, 11-13 mars 2015, Lille},

note = {ACTN},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Rorat2015,

title = {Assessment of the vermicomposting process applied to sewage sludge by monitoring of the compost quality and immune responses of earthworms},

author = {Agnieszka Rorat},

year  = {2015},

date = {2015-01-01},

booktitle = {Journée des Doctorants IRePSE, 08 juillet 2015, CERLA - Cité Scientifique - Université Lille 1, Villeneuve d'Ascq},

note = {ACTN},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@phdthesis{Rorat2015a,

title = {Assessment of the vermicomposting process applied to sewage sludge by monitoring of the compost quality and immune responses of three earthworm species: Eisenia fetida, Eisenia andrei and Dendrobaena veneta},

author = {Agnieszka Rorat},

year  = {2015},

date = {2015-01-01},

school = {Thèse en co-tuelle Ecole Polytechnique de Czestochowa, Pologne / Université de Lille 1, France, 188 pp},

abstract = {Évaluation du processus de lombricompostage appliqué aux boues d'épuration par le suivi de la qualité du compost et les réponses immunitaires de trois espèces de vers de terre : eisenia fetida, eisenia andrei et dendrobaena veneta résumé le vermicompostage est une éco-biotechnologie relativement nouvelle qui utilise des vers de terre comme bioréacteurs naturels dans un procéssus de décomposition de la matière organique. en europe, on utilise trois espèces d’annélides oligochètes : eisenia fetida, eisenia andrei et dendrobaena veneta. compte tenu des directives de l’union européenne portant sur le traitement des déchets, les contenus en métaux lourds, en composés chimiques divers et en microorganismes pathogènes des boues d’épuration ne permettent pas leur valorisation directe en agriculture. la qualité du produit obtenu après vermicompostage peut être évaluée en utilisant des paramètres agronomiques, tandis que des paramètres immunitaires et de défense mesurés chez les vers permettent d’apprécier l’impact des contaminants sur ces organismes du sol. les objectifs de ce travail étaient: 1) apprécier l'influence des vers de terre sur la qualité du produit final dans le processus de lombricompostage, 2) examiner les mécanismes moléculaires et immunologiques mis en œuvre chez les lombrics au cours du lombricompostage de boues d'épuration urbaines, 3) de développer le compostage combiné au processus de lombricompostage. les vers de terre utilisés dans les travaux présentés ont été classés en trois espèces grâce à la technique de barcoding moléculaire. les fluorophores sélectionnés ont été testés comme les biomarqueurs spécifiques à l’espèce. le contenu en riboflavine, le nombre de coelomocytes (amébocytes/éléocytes) et le niveau d’expression de gènes sélectionnés ont été utilisés comme biomarqueurs de stress permettant la bio-oisurvéillance du processus. la technique appliquée a conduit à évaluer les possibilités de valorisation des boues d'épuration.},

note = {TH},

keywords = {ER4},

pubstate = {published},

tppubtype = {phdthesis}

}

@article{Janus2015,

title = {Elaboration, characteristics and advantages of biochars for the management of contaminated soils with a specific overview on Miscanthus biochars},

author = {Adeline Janus and Aurélie Pelfrêne and Sophie Heymans and Christophe Deboffe and Francis Douay and Christophe Waterlot},

year  = {2015},

date = {2015-01-01},

journal = {Journal of Environmental Management},

volume = {162},

pages = {275-289},

abstract = {Biochars are products that are rich in carbon obtained by pyrolysis processes that consist in introducing a biomass (such as wood or manure) in a closed container and heating it with little or no available air. this paper reports the impacts of pyrolysis parameters on biochar characteristics. a preliminary examination of the scientific literature revealed that the type of feedstock, the temperature, the heating rate and the gas flow were the major parameters influencing the biochar characteristics. this review highlights the multitude of biochars that can be made and shows the importance of characterizing them before their use in soils. then we assess how the input of biochars in soils can affect soil parameters. a review of the literature showed modifications on: i) the physical properties of soils (i.e. the modification in soil structure and water retention), ii) the chemical properties of soils (i.e. the modification of ph, cation exchange capacity, nutrient availability, the organic matter content) and iii) the biological properties (i.e. the changes in microbial and faunal communities). all these modifications can lead to an increase in crop productivity, which confirms the value of biochars as a soil amendment. moreover, biochars can also provide an advantage for soil remediation. indeed, biochars efficiently reduce the bioavailability of organic and inorganic pollutants. in addition, this review focuses on a specific plant that can be used to produce biochars: miscanthus, a non-wood rhizomatous c4 perennial grass. miscanthus presents advantages for biochar production due to: i) its lignocellulosic content, ii) its silicon content, which can mitigate environmental stresses (notably for plants grown on contaminated sites) and iii) the greater surface area of the miscanthus biochars compared to the biochars produced with other feedstock},

note = {ACLS},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Janus2015a,

title = {Interest of Miscanthus biochars to decrease the bioavailability of metals and PAHs in aqueous solutions},

author = {Adeline Janus and Aurélie Pelfrêne and Sophie Heymans and Christophe Deboffe and Francis Douay and Christophe Waterlot},

year  = {2015},

date = {2015-01-01},

booktitle = {Joint International Biochar Symposium “Understanding Biochar Mechanisms for Pratical Implentation”, 28–30 September 2015, Geisenheim (Allemagne)},

note = {AFF},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Sahmer2015,

title = {Utilisation d'une régression non linéaire pour des applications microbiologiques},

author = {Karin Sahmer and Caroline Deweer and Jérôme Muchembled and Lucia Santorufo and Brice Louvel and Francis Douay and Patrice Halama},

year  = {2015},

date = {2015-01-01},

booktitle = {Séminaire Modal (MOdels for Data Analysis and Learning), INRIA, 13 janvier 2015, Lille},

note = {ACTN},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Labidi2015,

title = {Fly ash-aided phytostabilisation of highly trace element polluted soils improves the telluric fungal biomass},

author = {Sonia Labidi and Joël Fontaine and Frédéric Laruelle and Benoit Tisserant and Yolande Dalpé and Anne Grandmougin-Ferjani and Francis Douay and Anissa Lounès-Hadj-Sahraoui},

year  = {2015},

date = {2015-01-01},

journal = {Applied Soil Ecology},

volume = {85},

pages = {69-75},

abstract = {Ten years after fly ash (fa) amendments and tree mix plantation (black locust, black alder, sycamore maple, pedunculate oak and white willow), the viability of the telluric microorganisms in a highly trace element (te) polluted topsoil was studied. previous to tree plantation, three experimental plots were set up in the field: a non-amended plot (r), an amended plot with silico-aluminous fly ash (f1) and an amended plot with sulfo-calcic fly ash (f2). the arbuscular mycorrhizal fungi (amf), saprophytic fungal and bacterial biomasses were quantified by the measure of specific lipid markers (phospholipid fatty acids (plfa) and ergosterol). the highest amf root colonization was of 18% in the sub-plot of the plot (f1). the highest plfa c16:1v5 amount (2 nmol g1 soil), reported as a marker of the amf biomass, was recorded in the fly ash amended topsoil compared to the control (r). this result was in accordance with the highest number of amf spores isolated from the sub-plot of the plot f1. saprophytic and ectomycorrhizal fungal biomasses were estimated by measuring the plfa c18:2v6,9 and ergosterol amounts in the topsoil. similarly to the plfa c16:1v5 amounts, the highest plfa c18:2v6,9 amounts (4.7 nmol g1 soil) were observed in the fly ash amended topsoil compared to the control. however, no significant effect of fly ash amendments was observed on both gram-positive and gram-negative specific plfa. this study demonstrated the usefulness of fa amendments in the assisted phytostabilisation of te polluted topsoil through the enhancement of fungal population viability.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Santorufo2015,

title = {Responses of functional and taxonomic collembolan community structure to site management in Mediterranean urban and surrounding areas},

author = {Lucia Santorufo and Jerôme Cortet and Johanne Nahmani and Céline Pernin and Sandrine Salmon and Audrey Pernot and Jean-Louis Morel and Giulia Maisto},

year  = {2015},

date = {2015-01-01},

journal = {European Journal of Soil Biology},

volume = {70},

pages = {46-57},

abstract = {The aim of this study was to identify the physical and chemical properties of different human-mediated ecosystems (urban, peri-urban/industrial and agricultural – including forested areas as a control) and to evaluate their effects on the taxonomic and functional trait composition and microhabitat preferences of collembolan communities. soils impacted by different types of land management were sampled in urban and surrounding areas of naples, italy. the physical and chemical properties of the soil were measured, and the species distribution, functional traits and microhabitat preferences of its collembolan community were characterized. the results indicated that different types of human activities markedly affect the abiotic properties of soils. we found that agricultural soils were more altered than forest soils, and that collembolan communities in agricultural soil were dominated by few species (mainly proisotoma minuta and entomobrya multifasciata), and that these species showed adaptations to open or disturbed environments. instead, the collembolan communities in urban soils were comparable to those observed in forest soils. it appears that agricultural activities have a greater effect on the taxonomy and functional traits of collembolan communities than urban impact has.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Santos2015,

title = {The use of comet assay in plant toxicology: recent advances},

author = {Conceição-L.-V. Santos and Bertrand Pourrut and José-M.-P. Ferreira-de-Oliveira},

year  = {2015},

date = {2015-01-01},

journal = {Frontiers in Genetics},

volume = {6},

number = {216},

pages = {1-18},

abstract = {The systematic study of genotoxicity in plants induced by contaminants and other stress agents has been hindered to date by the lack of reliable and robust biomarkers. the comet assay is a versatile and sensitive method for the evaluation of dna damages and dna repair capacity at single-cell level. due to its simplicity and sensitivity, and the small number of cells required to obtain robust results, the use of plant comet assay has drastically increased in the last decade. for years its use was restricted to a few model species, e.g., allium cepa, nicotiana tabacum, vicia faba, or arabidopsis thaliana but this number largely increased in the last years. plant comet assay has been used to study the genotoxic impact of radiation, chemicals including pesticides, phytocompounds, heavy metals, nanoparticles or contaminated complex matrices. here we will review the most recent data on the use of this technique as a standard approach for studying the genotoxic effects of different stress conditions on plants. also, we will discuss the integration of information provided by the comet assay with other dna-damage indicators, and with cellular responses including oxidative stress, cell division or cell death. finally, we will focus on putative relations between transcripts related with dna damage pathways, dna replication and repair, oxidative stress and cell cycle progression that have been identified in plant cells with comet assays demonstrating dna damage},

note = {ACLO},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}