Publications

@techreport{Douay2011d,

title = {Site Metaleurop Nord à Noyelles-Godault (62). Campagnes de mesures des teneurs en plomb et en cadmium autour de l’ancien site industriel},

author = {Francis Douay and Julie Planque and Alexandre Loriette and Hervé Fourrier},

year  = {2011},

date = {2011-01-01},

abstract = {Ce présent rapport porte sur la réalisation d’une nouvelle campagne de mesures des concentrations en plomb et en cadmium dans les sols et les productions végétales qu’elles soient agricoles ou potagères dans la zone à plus de 200 mg de plomb kg‐1 autour de l’ancienne fonderie de plomb metaleurop nord (evin‐malmaison, courcelles les lens, noyelles‐godault, dourges, ostricourt, leforest et auby). la démarche vise à conforter ou redéfinir le périmètre du pig (établi en 1999) sur la base de données acquises sept ans après l’arrêt des activités de l’usine. les concentrations en plomb et en cadmium ont été mesurées dans l’horizon organo‐minéral (0 ‐ 25 cm) en 328 points répartis sur l’ensemble du secteur concerné (228 sols agricoles et 100 sols urbains). en parallèle, la teneur en ces deux éléments a été mesurée dans 303 prélèvements de végétaux (95 plantes agricoles et 208 plantes potagères). des semences et plants de variétés identiques ont été distribués aux jardiniers. les végétaux ont été préparés en fonction de leur destination et seules les parties habituellement consommées ont été analysées. la mesure des concentrations totales en pb et cd des sols et des végétaux a été confiée à l’inra. l’analyse statistique des concentrations totales en pb et en cd (campagne 2010 et campagnes antérieures) n’a pas mis en évidence une évolution notable du degré de contamination des sols depuis l’arrêt de l’activité. les sols présentent toujours aujourd’hui des concentrations totales en pb et cd qui dépassent d’une façon significative les teneurs agricoles habituelles régionales. l’analyse géostatistiques réalisée sur l’ensemble des informations acquises depuis l’arrêt des émissions de l’usine (plus de 1 000 données) atteste d’un fort degré de contamination de l’horizon organo‐minéral, d’une contamination plus importante des sols urbains et des habitats ligneux, de l’existence de « hot spots » de contamination (gare d’eau de courcelles les lens, site de stockage des boues de curage de la deûle). en comparaison des travaux antérieurs, l’accroissement du nombre de données analytiques a permis de préciser les courbes d’isoconcentration en pb et cd des sols. au regard de la réglementation en vigueur, la production agricole de végétaux pour l’alimentation humaine reste préoccupante dans la zone où les concentrations excèdent 4 mg de cadmium kg‐1 (grain de blé, d’escourgeon, féverole et betterave sucrière). la plupart des productions sont toutefois conformes pour l’alimentation animale (sauf les pailles de céréales et le maïs fourrager). la production de légumes au niveau des potagers est beaucoup plus problématique, ils sont souvent non‐conformes pour l’alimentation humaine. les plus cultivés (carotte, pomme de terre et poireau) sont d’ailleurs ceux qui présentent les plus forts taux de non‐conformité. de manière générale, la situation est plus préoccupante en ce qui concerne les concentrations totales en cd observées dans les végétaux qu’ils proviennent de l’agriculture ou des potagers. la situation environnementale a peu évolué depuis l’arrêt de l’activité de l’usine. des denrées alimentaires non‐conformes sont actuellement mises sur le marché. le dispositif en place devrait être renforcé pour garantir la sécurité alimentaire tout en assurant l’avenir de l’agriculture dans la zone concernée. les mesures de gestion devraient être étendues à partir de la zone qui présente des concentrations supérieures à 200 mg de pb kg‐1 et/ou à 4 mg de cd kg‐1.pour les sols urbains une stratégie de remédiation des sols selon leurs usages et les dangers pour les populations devrait être mise en place. attendues des populations locales, ces actions devraient être concomitantes d’un engagement des instances régionales, de la volonté et de la concertation des gestionnaires territoriaux.},

note = {VAL},

keywords = {ER4},

pubstate = {published},

tppubtype = {techreport}

}

@conference{Douay2011c,

title = {Fly ash-aided phytostabilisation of highly contaminated soils: assessment of field trial after 8 years},

author = {Francis Douay and Bertrand Pourrut and Christophe Waterlot and Alena Lopareva-Pohu and Christelle Pruvot and Guillaume Garçon and Géraldine Bidar and Pirouz Shirali},

year  = {2011},

date = {2011-01-01},

booktitle = {11th International Conference on the Biogeochemistry of Trace Elements (ICOBTE). 3-7 July 2011, Florence (Italie)},

note = {ACTI},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Pelfrene2011,

title = {In vitro digestion and DGT techniques for estimating cadmium and lead bioavailability in contaminated soils: Influence of gastric juice pH},

author = {Aurélie Pelfrêne and Christophe Waterlot and Francis Douay},

year  = {2011},

date = {2011-01-01},

journal = {Science of the Total Environment},

volume = {409},

pages = {5076-5085},

abstract = {A sensitivity analysis was conducted on an in vitro gastrointestinal digestion test (i) to investigate the influence of a low variation of gastric juice ph on the bioaccessibility of cd and pb in smelter-contaminated soils (fb, using the unified bioaccessibility method ubm) and fractions of metals that may be transported across the intestinal epithelium (fa, using the diffusive gradient in thin film technique), and (ii) to provide a better understanding of the significance of ph in health risk assessment through ingestion of soil by children. the risk of metal exposure to children (hazard quotient, hq) was determined for conditions that represent a worst-case scenario (i.e., ingestion rate of 200 mg day−1) using three separate calculations of metal daily intake: estimated daily intake (edi), bioaccessible edi (edi-fb), and oral bioavailable edi (edi-fa). the increasing ph from 1.2 to 1.7 resulted in: (i) no significant variation in cd-fb in the gastric phase but a decrease in the gastrointestinal phase; (ii) a decrease in soluble pb in the gastric phase and a significant variation in pb-fb in the gastrointestinal phase; (iii) a significant decrease in cd-fa and no variation in pb-fa; (iv) no change in edi-fb and edi-fa hqs for cd; (v) a significant decrease in edi-fb hqs and no significant variation in edi-fa hq for pb. in the analytical conditions, these results show that risk to children decreases when the bioavailability of pb in soils is taken into account and that the studied ph values do not affect the edi-fa hqs. the present results provide evidence that the inclusion of bioavailability analysis during health risk assessment could provide a more realistic estimate of cd and pb exposure, and opens a wide field of practical research on this topic (e.g., in contaminated site management).},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Pelfrene2011a,

title = {Investigation of DGT as a metal speciation tool in artificial human gastrointestinal fluids},

author = {Aurélie Pelfrêne and Christophe Waterlot and Francis Douay},

year  = {2011},

date = {2011-01-01},

journal = {Analytica Chimica Acta},

volume = {699},

pages = {177-186},

abstract = {This paper reports the results of an investigation on the performance of the diffusive gradient in thin film technique (dgt) as a speciation tool for trace elements (tes) in artificial human gastrointestinal fluids. the validity of cd, pb, and zn sampling by dgt in digestive fluids was checked. the te bioaccessibility in highly contaminated soils was determined using the in vitro unified barge method (ubm) test. dgt devices were deployed in the gastrointestinal solutions obtained after carrying out the ubm test. the computer speciation code jess (joint expert speciation system) was used to predict the metal speciation of cd, pb, and zn. combining the in vitro test with the dgt technique and jess provided an approach to the te species available for transport across the intestinal epithelium. the gastrointestinal absorption of ingested te ranged from 8 to 30% for cd, 0.6 to 11% for pb, and 0.8 to 7% for zn and was influenced by te speciation. in this original approach, the dgt technique was found to be simple and reliable in the investigation of te chemical speciation in digestive fluids. extrapolation to the in vivo situation should be undertaken very cautiously and requires further investigation.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Pelfrene2011b,

title = {Assessing Cd, Pb, Zn mobility and human bioaccessibility in smelter-contaminated agricultural topsoils (northern France)},

author = {Aurélie Pelfrêne and Christophe Waterlot and Muriel Mazzuca and Catherine Nisse and Géraldine Bidar and Francis Douay},

year  = {2011},

date = {2011-01-01},

journal = {Environmental Geochemistry and Health},

volume = {33},

number = {5},

pages = {477-493},

abstract = {The extractability of cd, pb, and zn was investigated in contaminated agricultural topsoils located in an area highly affected by the past atmospheric emissions of two smelters in northern france in order to assess their mobility and human bioaccessibility. the determination of cd, pb, and zn bioaccessibility (unified barge method, in vitro test) was made to evaluate the absolute trace element (te) bioavailability. the results highlighted differences in bioaccessibility between cd, pb, and zn (cd[pb[ zn). the mean values of the bioaccessible fractions of cd, pb, and zn during the gastric phase were 82, 55, and 33%, respectively, of the pseudototal concentrations, whereas during the gastrointestinal phase, the bioaccessible fractions of metals decreased to 45, 20, and 10%, respectively. stepwise multiple regression analysis showed that human bioaccessibility was affected by various physicochemical parameters (i.e., sand, carbonates, organic matter, assimilated p, free al oxides, and pseudototal fe contents). sequential extractions were performed as an indication of the te availability in these soils. cadmium occurred in the more available fractions, pb was mostly present as bound by oxides, and a significant contribution to the pseudototal zn concentration was defined as the unavailable residual form related to the crystalline structures of minerals. the concepts of bioavailability and bioaccessibility are important for quantifying the risks associated with exposure to environmental pollutants and providing more realistic information for human health},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Pelfrene2011c,

title = {Assessing Cd and Pb human bioaccessibility in smelter-contaminated agricultural and urban soils (northern France)},

author = {Aurélie Pelfrêne and Christophe Waterlot and Catherine Nisse and Muriel Mazzuca and Francis Douay},

year  = {2011},

date = {2011-01-01},

booktitle = {11th International Conference on the Biogeochemistry of Trace Elements (ICOBTE). 3-7 July 2011, Florence (Italy)},

note = {ACTI},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Fritsch2011,

title = {Spatially explicit analysis of metal transfer to biota: influence of soil contamination and landscape},

author = {Clémentine Fritsch and Michaël Coeurdassier and Patrick Giraudoux and Francis Douay and Dominique Rieffel and Annette De-Vaufleury and Renaud Scheifler},

year  = {2011},

date = {2011-01-01},

journal = {PLoS ONE},

volume = {6},

number = {5},

pages = {e20682 (p 1-16)},

abstract = {Concepts and developments for a new field in ecotoxicology, referred to as ‘‘landscape ecotoxicology,’’ were proposed in the 1990s; however, to date, few studies have been developed in this emergent field. in fact, there is a strong interest in developing this area, both for renewing the concepts and tools used in ecotoxicology as well as for responding to practical issues, such as risk assessment. the aim of this study was to investigate the spatial heterogeneity of metal bioaccumulation in animals in order to identify the role of spatially explicit factors, such as landscape as well as total and extractable metal concentrations in soils. over a smelter-impacted area, we studied the accumulation of trace metals (tms: cd, pb and zn) in invertebrates (the grove snail cepaea sp and the glass snail oxychilus draparnaudi) and vertebrates (the bank vole myodes glareolus and the greater white-toothed shrew crocidura russula). total and cacl2-extractable concentrations of tms were measured in soils from woody patches where the animals were captured. tm concentrations in animals exhibited a high spatial heterogeneity. they increased with soil pollution and were better explained by total rather than cacl2-extractable tm concentrations, except in cepaea sp. tm levels in animals and their variations along the pollution gradient were modulated by the landscape, and this influence was species and metal specific. median soil metal concentrations (predicted by universal kriging) were calculated in buffers of increasing size and were related to bioaccumulation. the spatial scale at which tm concentrations in animals and soils showed the strongest correlations varied between metals, species and landscapes. the potential underlying mechanisms of landscape influence (community functioning, behaviour, etc.) are discussed. present results highlight the need for the further development of landscape ecotoxicology and multi-scale approaches, which would enhance our understanding of pollutant transfer and effects in ecosystems.},

note = {ACLO},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Peres2011,

title = {Selection of soil bioindicators for impact assessment of land use changes and soil protection. Exemple of the "Bioindicators Programme."},

author = {Guénola Pérès and Antonio Bispo and Cécile Grand and Isabelle Gattin and Mickaël Hedde and Jennifer Harris-Hellal and Marina Le-Guédard and Nuria Ruiz and A Alaphilippe and Thierry Béguiristain and Francis Douay and Olivier Faure and Adnane Hitmi and Sabine Houot and Marc Legras and Muriel Guernion and Jean-François Vian and Laurence Rougé and Jeanne-Chantal Dur and Daniel Cluzeau},

year  = {2011},

date = {2011-01-01},

booktitle = {The Wageningen Conference on Applied Soil Science. 18-22 September 2011, Wageningen (the Netherlands)},

note = {COM},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Peres2011a,

title = {A national research programme to validate a battery of soil bioindicators for impact and risk assessment in urban soils},

author = {Guénola Pérès and Cécile Grand and Isabelle Gattin and Mickaël Hedde and Jennifer Harris-Hellal and Marina Le-Guédard and Nuria Ruiz and A Alaphilippe and Thierry Béguiristain and Christelle Pruvot and Olivier Faure and Adnane Hitmi and Sabine Houot and Marc Legras and Muriel Guernion and Jean-François Vian and S Conil and Laurence Rougé and Salima Taibi and Daniel Cluzeau},

year  = {2011},

date = {2011-01-01},

booktitle = {SUITMA 6. 3-7 October 2011, Marrakech (Maroc)},

note = {COM},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Gineys2011c,

title = {Managing trace elements in Portland cement – Part II: Comparison of two methods to incorporate Zn in a cement},

author = {Nathalie Gineys and Georges Aouad and Denis Damidot},

year  = {2011},

date = {2011-01-01},

journal = {Cement & Concrete Composites},

volume = {33},

number = {6},

pages = {629-636},

abstract = {This study compares two methods to immobilise the same amount of zn in cement paste. in the first method, zn is introduced into the raw material before clinkerisation, whereas in the second process, zn is introduced in the liquid phase used to make the cement paste, which is often referred to as solidification/stabilisation. in laboratory-made clinker, zn was mainly fixed within a new compound (ca6zn3al4o15). also, zn was observed in tricalcium silicate (c3s) and in the interstitial phase (c3a and c4af) with the exception of dicalcium silicate (c2s). in the present study, the threshold limit value corresponds to the maximum amount of zn that could be incorporated without the stability of the clinker phases being modified. this threshold was reached when a decrease in c3a content and/or the presence of ca6zn3al4o15 were observed by x-ray diffraction. the threshold limit of zn in the studied clinker was established at 0.7% by weight. the reactivity of synthesised cement doped with zn was studied by isothermal calorimetry measurements. these tests revealed that the incorporation of zn had no effect on calcium silicate hydration (c3s and c2s), even if the zn content exceeded the threshold limit value, i.e., 1%. these results were compared with those obtained through the solidification/stabilisation treatment of zn in a cement paste. in these experiments, a zn content of 0.7% induced a significant delay in the cement hydration. this difference in behaviour is due to the slower flow of zinc ions released in solution when it is incorporated in the clinker phases. thus, the immobilisation during clinkerisation is a good alternative to treat waste with a high zn content in ordinary portland cement (opc) without causing the well-known, deleterious effect on cement setting time},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Godet2011,

title = {Growth and metal accumulation in Porcellio scaber exposed to poplar litter from Cd-, Pb- and Zn-contaminated sites},

author = {Jean-Philippe Godet and Sylvain Demuynck and Christophe Waterlot and Sébastien Lemière and Catherine Souty-Grosset and Renaud Scheifler and Francis Douay and Alain Leprêtre and Christelle Pruvot},

year  = {2011},

date = {2011-01-01},

journal = {Ecotoxicology and Environmental Safety},

volume = {74},

number = {3},

pages = {451-458},

abstract = {This study aimed at determining, in the laboratory, the effects of poplar litter collected in woody habitats contaminated by heavy metals on growth and metal accumulation in the terrestrial isopod porcellio scaber. cd, pb, and zn pseudototal and cacl2-extractable concentrations in litter types were determined using aas. juveniles were fed ad libitum, individually, for 28 days with four litter types presenting an increasing gradient of metal contamination. individuals were weighed every week and metal body burdens were determined at the end of the experiment. from the first week until the end of the experiment, a decrease in p. scaber growth related to the increase of metal concentration in litter types was recorded. significant correlations were observed between metal body burdens and metal concentrations in litter types. however, cd accumulation in woodlice appeared to be related to the cd/zn concentration ratio in litter types. all these results showed the potential of weight gain in p. scaber as a suitable indicator for litter quality assessment with ecological relevance.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Pourrut2011,

title = {Lead-induced DNA damage in Vicia faba root cells: Potential involvement of oxidative stress},

author = {Bertrand Pourrut and Séverine Jean and Jérôme Silvestre and Eric Pinelli},

year  = {2011},

date = {2011-01-01},

journal = {Mutation Research},

volume = {726},

number = {2},

pages = {123-128},

abstract = {Genotoxic effects of lead (0–20 μm) were investigated in whole-plant roots of vicia faba l., grown hydroponically under controlled conditions. lead-induced dna damage in v. faba roots was evaluated by use of the comet assay, which allowed the detection of dna strand-breakage and with the v. faba micronucleus test, which revealed chromosome aberrations. the results clearly indicate that lead induced dna fragmentation in a dose-dependant manner with a maximum effect at 10 μm. in addition, at this concentration, dna damage time-dependently increased until 12 h. then, a decrease in dna damages was recorded. the significant induction of micronucleus formation also reinforced the genotoxic character of this metal. direct interaction of lead with dna was also evaluated with the a-cellular comet assay. the data showed that dna breakages were not associated with a direct effect of lead on dna. in order to investigate the relationship between lead genotoxicity and oxidative stress, v. faba were exposed to lead in the presence or absence of the antioxidant vitamin e, or the nadph-oxidase inhibitor dephenylene iodonium (dpi). the total inhibition of the genotoxic effects of lead (dna breakage and micronucleus formation) by these compounds reveals the major role of reactive oxygen species (ros) in the genotoxicity of lead. these results highlight, for the first time in vivo and in whole-plant roots, the relationship between ros, dna strand-breaks and chromosome aberrations induced by lead},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Pourrut2011c,

title = {The PHYTENER project: development of phytostabilisation combined with energy crop production on agricultural soils highly contaminated by metals},

author = {Bertrand Pourrut and Alain Leprêtre and Eric Therssen and Annabelle Deram and Pirouz Shirali and Fabrice Cazier and Antoine Richard and Renaud Scheifler and Jacques Blarel and Francis Douay and the-PHYTENER-Team},

year  = {2011},

date = {2011-01-01},

booktitle = {European Geosciences Union General Assembly. 3-8 April 2011, Vienna (Austria)},

volume = {13},

pages = {1-2},

note = {ACTI},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Pourrut2011a,

title = {Assessment of fly ash-aided phytostabilisation of highly contaminated soils after an 8-year field trial. Part 2. Influence on plants},

author = {Bertrand Pourrut and Alena Lopareva-Pohu and Christelle Pruvot and Guillaume Garçon and Anthony Verdin and Christophe Waterlot and Géraldine Bidar and Pirouz Shirali and Francis Douay},

year  = {2011},

date = {2011-01-01},

journal = {Science of the Total Environment},

volume = {409},

pages = {4504-4510},

abstract = {Aided phytostabilisation is a cost-efficient technique to manage metal-contaminated areas, particularly in the presence of extensive pollution. plant establishment and survival in highly metal-contaminated soils are crucial for phytostabilisation success, as metal toxicity for plants is widely reported. a relevant phytostabilisation solution must limit metal transfer through the food chain. therefore, this study aimed at evaluating the long-term efficiency of aided phytostabilisation on former agricultural soils highly contaminated by cadmium, lead, and zinc. the influence of afforestation and fly ash amendments on reducing metal phytoavailability was investigated as were their effects on plant development. before being planted with a tree mix, the site was divided into three plots: a reference plot with no amendment, a plot amended with silico-aluminous fly ash and one with sulfo-calcic fly ash. unlike salix alba and quercus robur, alnus glutinosa, acer pseudoplatanus and robinia pseudoacacia grew well on the site and accumulated, overall, quite low concentrations of metals in their leaves and young twigs. this suggests that these three species have an excluder phenotype for cd, zn and pb. after 8 years, metal availability to a. glutinosa, a. pseudoplatanus and r. pseudoacacia, and translocation to their above-ground parts, strongly decreased in fly ash-amended soils. such decreases fit well together with the depletion of cacl2-extractable metals in amended soils. although both fly ashes were effective to decrease cd, pb and zn concentrations in above-ground parts of trees, the sulfo-calcic ash was more efficient.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Pourrut2011d,

title = {Recommendations for conducting the alkaline comet assay in higher plants},

author = {Bertrand Pourrut and Eric Pinelli and Jérôme Silvestre and Francis Douay},

year  = {2011},

date = {2011-01-01},

booktitle = {9th International Comet Assay Workshop (ICAW). 13-16 September 2011, Kusadası (Turquie)},

note = {ACTI},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Pourrut2011b,

title = {Lead uptake, toxicity, and detoxification in plants},

author = {Bertrand Pourrut and Muhammad Shahid and Camille Dumat and Peter Winterton and Eric Pinelli},

year  = {2011},

date = {2011-01-01},

journal = {Reviews of Environmental Contamination and Toxicology},

volume = {213},

pages = {113-136},

abstract = {Plants are the target of a wide range of pollutants that vary in concentration, speciation, and toxicity. such pollutants mainly enter the plant system through the soil (arshad et al. 2008) or via the atmosphere (uzu et al. 2010). among common pollutants that affect plants, lead is among the most toxic and frequently encountered (cecchi et al. 2008; grover et al. 2010; shahid et al. 2011). lead continues to be used widely in many industrial processes and occurs as a contaminant in all environmental compartments (soils, water, the atmosphere, and living organisms). the prominence of environmental lead contamination results both from its persistence (islam et al. 2008; andra et al. 2009; punamiya et al. 2010) and from its present and past numerous sources. these sources have included smelting, combustion of leaded gasoline, or applications of lead-contaminated media (sewage sludge and fertilizers) to land (piotrowska et al. 2009; gupta et al. 2009; sammut et al. 2010; grover et al. 2010). in 2009, production of recoverable lead from mining operations was 1690, 516, and 400 thousand metric tons by china, australia, and the usa, respectively (usgs 2009).},

note = {ACLS},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Pruvot2011,

title = {The use of fluctuating asymmetry analysis to evaluate the effects of metal-pollution (Cd, Pb, Zn) on Porcellio scaber (Crustacea, Isopoda) living in contaminated woody habitats},

author = {Christelle Pruvot and Jean-Philippe Godet and Sylvain Demuynck and Catherine Souty-Grosset and Francis Douay and Alain Leprêtre},

year  = {2011},

date = {2011-01-01},

booktitle = {12th European Ecological Federation Congress. 25-29 September 2011, Avila (Espagne)},

note = {ACTI},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}