2005Conférence ER4 Auteurs : Brulle, Franck; Mitta, Guillaume; Cocquerelle, Claude; Vieau, Didier; Lemière, Sébastien; Leprêtre, Alain; Vandenbulcke, Franck Identification, characterization and validation of pollution biomarkers in soil invertebrates Ecotox 2005. Advances and Trends in Ecotoxicology. 5-7 September 2005, Brno (Czech Republic), 2005, (COM). @conference{Brulle2005,
title = {Identification, characterization and validation of pollution biomarkers in soil invertebrates},
author = {Franck Brulle and Guillaume Mitta and Claude Cocquerelle and Didier Vieau and Sébastien Lemière and Alain Leprêtre and Franck Vandenbulcke},
year = {2005},
date = {2005-01-01},
booktitle = {Ecotox 2005. Advances and Trends in Ecotoxicology. 5-7 September 2005, Brno (Czech Republic)},
note = {COM},
keywords = {ER4},
pubstate = {published},
tppubtype = {conference}
}
|
2004Conférence ER4 Auteurs : Gonzalez, Marcelo; Gueguen, Yannick; Fievet, Julie; Vandenbulcke, Franck; Bachère, Evelyne Molecular characterization of an immune related gene, CgBP/LBP isolated from the oyster Crassostrea gigas Aquaculture Europe 2004: Biotechnologies for quality. 20-23 October 2004, Barcelona (Espagne), 2004, (ACTI). @conference{Gonzalez2004,
title = {Molecular characterization of an immune related gene, CgBP/LBP isolated from the oyster Crassostrea gigas},
author = {Marcelo Gonzalez and Yannick Gueguen and Julie Fievet and Franck Vandenbulcke and Evelyne Bachère},
year = {2004},
date = {2004-01-01},
booktitle = {Aquaculture Europe 2004: Biotechnologies for quality. 20-23 October 2004, Barcelona (Espagne)},
note = {ACTI},
keywords = {ER4},
pubstate = {published},
tppubtype = {conference}
}
|
2004Article de journal ER4 Auteurs : Lefebvre, Christophe; Cocquerelle, Claude; Vandenbulcke, Franck; Hot, David; Huot, Ludovic; Lemoine, Yves; Salzet, Michel Transcriptomic analysis in the leech Theromyzon tessulatum: Involvement of cystatin B in innate immunity Dans: Biochemical Journal, vol. 380, no. 3, p. 617-625, 2004, (ACL). Résumé @article{Lefebvre2004,
title = {Transcriptomic analysis in the leech Theromyzon tessulatum: Involvement of cystatin B in innate immunity},
author = {Christophe Lefebvre and Claude Cocquerelle and Franck Vandenbulcke and David Hot and Ludovic Huot and Yves Lemoine and Michel Salzet},
year = {2004},
date = {2004-01-01},
journal = {Biochemical Journal},
volume = {380},
number = {3},
pages = {617-625},
abstract = {At the present time, there is little information on mechanisms of innate immunity in invertebrate groups other than insects, especially annelids. in the present study, we have performed a transcriptomic study of the immune response in the leech theromyzon tessulatum after bacterial challenge, by a combination of differential display rt (reverse transcriptase)–pcr and cdna microarrays. the results show relevant modulations concerning several known and unknown genes. indeed, threonine deaminase, malate dehydrogenase, cystatin b, polyadenylate-binding protein and α-tubulin-like genes are up-regulated after immunostimulation. we focused on cystatin b (stefin b), which is an inhibitor of cysteine proteinases involved in the vertebrate immune response.we have cloned the full-length cdna and named the t. tessulatum gene as tt-cysb. main structural features of cystatins were identified in the derived amino acid sequence of tt-cysb cdna; namely, a glycine residue in the n-terminus and a consensus sequence of gln-xaa-val-xaa-gly (qxvxg) corresponding to the catalytic site. moreover, tt-cysb is the first cystatin b gene characterized in invertebrates. we have determined by in situ hybridization and immunocytochemistry that tt-cysb is only expressed in large coelomic cells. in addition, this analysis confirmed that tt-cysb is up-regulated after bacterial challenge, and that increased expression occurs only in coelomic cells. these data demonstrate that the innate immune response in the leech involves a cysteine proteinase inhibitor that is not found in ecdysozoan models, such as drosophila melanogaster or caenorhabditis elegans, and so underlines the great need for information about innate immunity mechanisms in different invertebrate groups.},
note = {ACL},
keywords = {ER4},
pubstate = {published},
tppubtype = {article}
}
At the present time, there is little information on mechanisms of innate immunity in invertebrate groups other than insects, especially annelids. in the present study, we have performed a transcriptomic study of the immune response in the leech theromyzon tessulatum after bacterial challenge, by a combination of differential display rt (reverse transcriptase)–pcr and cdna microarrays. the results show relevant modulations concerning several known and unknown genes. indeed, threonine deaminase, malate dehydrogenase, cystatin b, polyadenylate-binding protein and α-tubulin-like genes are up-regulated after immunostimulation. we focused on cystatin b (stefin b), which is an inhibitor of cysteine proteinases involved in the vertebrate immune response.we have cloned the full-length cdna and named the t. tessulatum gene as tt-cysb. main structural features of cystatins were identified in the derived amino acid sequence of tt-cysb cdna; namely, a glycine residue in the n-terminus and a consensus sequence of gln-xaa-val-xaa-gly (qxvxg) corresponding to the catalytic site. moreover, tt-cysb is the first cystatin b gene characterized in invertebrates. we have determined by in situ hybridization and immunocytochemistry that tt-cysb is only expressed in large coelomic cells. in addition, this analysis confirmed that tt-cysb is up-regulated after bacterial challenge, and that increased expression occurs only in coelomic cells. these data demonstrate that the innate immune response in the leech involves a cysteine proteinase inhibitor that is not found in ecdysozoan models, such as drosophila melanogaster or caenorhabditis elegans, and so underlines the great need for information about innate immunity mechanisms in different invertebrate groups. |
2004Article de journal ER4 Auteurs : Munoz, Marcelo; Vandenbulcke, Franck; Garnier, Julien; Gueguen, Yannick; Bulet, Philippe; Saulnier, Denis; Bachère, Evelyne Involvement of penaeidins in defense reactions of the shrimp Litopenaeus stylirostris to a pathogenic vibrio Dans: Cellular and Molecular Life Sciences, vol. 61, no. 7-8, p. 961-972, 2004, (ACL). @article{Munoz2004,
title = {Involvement of penaeidins in defense reactions of the shrimp Litopenaeus stylirostris to a pathogenic vibrio},
author = {Marcelo Munoz and Franck Vandenbulcke and Julien Garnier and Yannick Gueguen and Philippe Bulet and Denis Saulnier and Evelyne Bachère},
year = {2004},
date = {2004-01-01},
journal = {Cellular and Molecular Life Sciences},
volume = {61},
number = {7-8},
pages = {961-972},
note = {ACL},
keywords = {ER4},
pubstate = {published},
tppubtype = {article}
}
|
2004Article de journal ER4 Auteurs : Rivière, Guillaume; Michaud, Annie; Deloffre, Laurence; Vandenbulcke, Franck; Levoye, Angélique; Breton, Christophe; Corvol, Pierre; Salzet, Michel; Vieau, Didier Characterization of the first non-insect invertebrate functional angiotensin-converting enzyme (ACE): Leech TtACE resembles the N-domain of mammalian ACE Dans: Biochemical Journal, vol. 382, no. 2, p. 565-573, 2004, (ACL). Résumé @article{Riviere2004,
title = {Characterization of the first non-insect invertebrate functional angiotensin-converting enzyme (ACE): Leech TtACE resembles the N-domain of mammalian ACE},
author = {Guillaume Rivière and Annie Michaud and Laurence Deloffre and Franck Vandenbulcke and Angélique Levoye and Christophe Breton and Pierre Corvol and Michel Salzet and Didier Vieau},
year = {2004},
date = {2004-01-01},
journal = {Biochemical Journal},
volume = {382},
number = {2},
pages = {565-573},
abstract = {Angiotensin-converting enzyme (ace) is a zinc metallopeptidase that plays a major role in blood homoeostasis and reproduction in mammals. in vertebrates, both transmembrane and soluble ace, containing one or two homologous active sites, have been characterized. so far, several aces from invertebrates have been cloned, but only in insects. they are soluble and display a single active site. using biochemical procedures, an ace-like activity was detected in our model, the leech, theromyzon tessulatum. annelida is the most distant phylum in which an ace activity has been observed. to gain more insight into the leech enzyme, we have developed a pcr approach to characterize its mrna. the approx. 2 kb cdna has been predicted to encode a 616-amino-acid soluble enzyme containing a single active site, named ttace (t. tessulatum ace). surprisingly, its primary sequence shows greater similarity to vertebrates than to invertebrates. stable in vitro expression of ttace in transfected chinese-hamster ovary cells revealed that the leech enzyme is a functional metalloprotease. as in mammals, this 79 kda glycosylated enzyme functions as a dipeptidyl carboxypeptidase capable of hydrolysing angiotensin i to angiotensin ii. however, a weak chloride inhibitory effect and acetylated n-acetyl-sdkp (ac sdackp) hydrolysis reveal that ttace activity resembles that of the n-domain of mammalian ace. in situ hybridization shows that its cellular distribution is restricted to epithelial midgut cells. although the precise roles and endogenous substrates of ttace remain to be identified, characterization of this ancestral peptidase will help to clarify its physiological roles in non-insect invertebrate species.},
note = {ACL},
keywords = {ER4},
pubstate = {published},
tppubtype = {article}
}
Angiotensin-converting enzyme (ace) is a zinc metallopeptidase that plays a major role in blood homoeostasis and reproduction in mammals. in vertebrates, both transmembrane and soluble ace, containing one or two homologous active sites, have been characterized. so far, several aces from invertebrates have been cloned, but only in insects. they are soluble and display a single active site. using biochemical procedures, an ace-like activity was detected in our model, the leech, theromyzon tessulatum. annelida is the most distant phylum in which an ace activity has been observed. to gain more insight into the leech enzyme, we have developed a pcr approach to characterize its mrna. the approx. 2 kb cdna has been predicted to encode a 616-amino-acid soluble enzyme containing a single active site, named ttace (t. tessulatum ace). surprisingly, its primary sequence shows greater similarity to vertebrates than to invertebrates. stable in vitro expression of ttace in transfected chinese-hamster ovary cells revealed that the leech enzyme is a functional metalloprotease. as in mammals, this 79 kda glycosylated enzyme functions as a dipeptidyl carboxypeptidase capable of hydrolysing angiotensin i to angiotensin ii. however, a weak chloride inhibitory effect and acetylated n-acetyl-sdkp (ac sdackp) hydrolysis reveal that ttace activity resembles that of the n-domain of mammalian ace. in situ hybridization shows that its cellular distribution is restricted to epithelial midgut cells. although the precise roles and endogenous substrates of ttace remain to be identified, characterization of this ancestral peptidase will help to clarify its physiological roles in non-insect invertebrate species. |
2004Conférence ER4 Auteurs : Sautière, Pierre-Eric; Vergote, David; Vandenbulcke, Franck; Mitta, Guillaume; Vizioli, Jacopo; Macagno, Eduardo-R.; Salzet, Michel Leech central nervous system response following septic challenge: a proteomic approach First international meeting on Evolution, Development and neurobiology of the leech. 29 septembre-3 octobre 2004, Villeneuve d’Ascq, 2004, (ACTI). @conference{Sautiere2004,
title = {Leech central nervous system response following septic challenge: a proteomic approach},
author = {Pierre-Eric Sautière and David Vergote and Franck Vandenbulcke and Guillaume Mitta and Jacopo Vizioli and Eduardo-R. Macagno and Michel Salzet},
year = {2004},
date = {2004-01-01},
booktitle = {First international meeting on Evolution, Development and neurobiology of the leech. 29 septembre-3 octobre 2004, Villeneuve d’Ascq},
note = {ACTI},
keywords = {ER4},
pubstate = {published},
tppubtype = {conference}
}
|
2004Article de journal ER4 Auteurs : Tasiemski, Aurélie; Vandenbulcke, Franck; Mitta, Guillaume; Lemoine, Jérôme; Lefebvre, Christophe; Sautière, Pierre-Eric; Salzet, Michel Molecular characterization of two novel antibacterial peptides inducible upon bacterial challenge in an annelid, the leech Theromyzon tessulatum Dans: Journal of Biological Chemistry, vol. 279, no. 30, p. 30973-30982, 2004, (ACL). @article{Tasiemski2004,
title = {Molecular characterization of two novel antibacterial peptides inducible upon bacterial challenge in an annelid, the leech Theromyzon tessulatum},
author = {Aurélie Tasiemski and Franck Vandenbulcke and Guillaume Mitta and Jérôme Lemoine and Christophe Lefebvre and Pierre-Eric Sautière and Michel Salzet},
year = {2004},
date = {2004-01-01},
journal = {Journal of Biological Chemistry},
volume = {279},
number = {30},
pages = {30973-30982},
note = {ACL},
keywords = {ER4},
pubstate = {published},
tppubtype = {article}
}
|
2004Conférence ER4 Auteurs : Tasiemski, Aurélie; Vandenbulcke, Franck; Mitta, Guillaume; Lemoine, Jérôme; Lefebvre, Christophe; Sautière, Pierre-Eric; Salzet, Michel Molecular characterization of two novel antibacterial peptides inducible upon bacterial challenge in an annelid, the leech Theromyzon tessulatum First international meeting on Evolution, Development and neurobiology of the leech. 29 septembre-3 octobre 2004, Villeneuve d’Ascq, France, 2004, (ACTI). @conference{Tasiemski2004a,
title = {Molecular characterization of two novel antibacterial peptides inducible upon bacterial challenge in an annelid, the leech Theromyzon tessulatum},
author = {Aurélie Tasiemski and Franck Vandenbulcke and Guillaume Mitta and Jérôme Lemoine and Christophe Lefebvre and Pierre-Eric Sautière and Michel Salzet},
year = {2004},
date = {2004-01-01},
booktitle = {First international meeting on Evolution, Development and neurobiology of the leech. 29 septembre-3 octobre 2004, Villeneuve d’Ascq, France},
note = {ACTI},
keywords = {ER4},
pubstate = {published},
tppubtype = {conference}
}
|
2004Conférence ER4 Auteurs : Vandenbulcke, Franck Necessity to explore innate immunity in diverse models: the leech Theromyzon tessulatum XIX International Congress of Zoology. 23-27 August 2004, Beijing (China), 2004, (INV). @conference{Vandenbulcke2004,
title = {Necessity to explore innate immunity in diverse models: the leech Theromyzon tessulatum},
author = {Franck Vandenbulcke},
year = {2004},
date = {2004-01-01},
booktitle = {XIX International Congress of Zoology. 23-27 August 2004, Beijing (China)},
note = {INV},
keywords = {ER4},
pubstate = {published},
tppubtype = {conference}
}
|
2004Article de journal ER4 Auteurs : Vergote, David; Sautière, Pierre-Eric; Vandenbulcke, Franck; Vieau, Didier; Mitta, Guillaume; Macagno, Eduardo-R.; Salzet, Michel Up-regulation of neurohemerythrin expression in the central nervous system of the medicinal leech, Hirudo medicinalis, following septic injury Dans: Journal of Biological Chemistry, vol. 279, no. 42, p. 43828-43837, 2004, (ACL). @article{Vergote2004,
title = {Up-regulation of neurohemerythrin expression in the central nervous system of the medicinal leech, Hirudo medicinalis, following septic injury},
author = {David Vergote and Pierre-Eric Sautière and Franck Vandenbulcke and Didier Vieau and Guillaume Mitta and Eduardo-R. Macagno and Michel Salzet},
year = {2004},
date = {2004-01-01},
journal = {Journal of Biological Chemistry},
volume = {279},
number = {42},
pages = {43828-43837},
note = {ACL},
keywords = {ER4},
pubstate = {published},
tppubtype = {article}
}
|
2003Conférence ER4 Auteurs : Bachère, Evelyne; Destoumieux-Garzón, Delphine; Munoz, Marcelo; Vandenbulcke, Franck; Bulet, Philippe Penaeidins, a family of antimicrobial peptides ubiquitous in Crustacea: involvement in shrimp immune response to infections 4th International Gordon Research Conference on Antimicrobial Peptides, 27 April-2 May 2003, Barga (Italie), 2003, (ACTI). @conference{Bachere2003,
title = {Penaeidins, a family of antimicrobial peptides ubiquitous in Crustacea: involvement in shrimp immune response to infections},
author = {Evelyne Bachère and Delphine Destoumieux-Garzón and Marcelo Munoz and Franck Vandenbulcke and Philippe Bulet},
year = {2003},
date = {2003-01-01},
booktitle = {4th International Gordon Research Conference on Antimicrobial Peptides, 27 April-2 May 2003, Barga (Italie)},
note = {ACTI},
keywords = {ER4},
pubstate = {published},
tppubtype = {conference}
}
|
2003Conférence ER4 Auteurs : Bachère, Evelyne; Munoz, Marcelo; Vandenbulcke, Franck; Bulet, Philippe; Garnier, Julien; Fievet, Julie; Dietrich, Jacques; Romestand, Bernard; Gueguen, Yannick Penaeidines, une famille de peptides antimicrobiens: implication dans la réponse immunitaire Réunion Immunologie des Invertébrés. 17-19 novembre 2003, Banyuls, 2003, (ACTN). @conference{Bachere2003a,
title = {Penaeidines, une famille de peptides antimicrobiens: implication dans la réponse immunitaire},
author = {Evelyne Bachère and Marcelo Munoz and Franck Vandenbulcke and Philippe Bulet and Julien Garnier and Julie Fievet and Jacques Dietrich and Bernard Romestand and Yannick Gueguen},
year = {2003},
date = {2003-01-01},
booktitle = {Réunion Immunologie des Invertébrés. 17-19 novembre 2003, Banyuls},
note = {ACTN},
keywords = {ER4},
pubstate = {published},
tppubtype = {conference}
}
|
2003Conférence ER4 Auteurs : Gonzalez, Marcelo; Gueguen, Yannick; Escoubas, Jean-Michel; Dietrich, Jacques; Fievet, Julie; Vandenbulcke, Franck; Bachère, Evelyne Identification de la première protéine de type LBP/BPI chez un invertébré, l’huitre creuse Crassotrea gigas: étude de l’expression du gène et caractérisation préliminaire de la protéine recombinante Réunion Immunologie des Invertébrés. 17-19 novembre 2003, Banyuls, 2003, (ACTN). @conference{Gonzalez2003,
title = {Identification de la première protéine de type LBP/BPI chez un invertébré, l’huitre creuse Crassotrea gigas: étude de l’expression du gène et caractérisation préliminaire de la protéine recombinante},
author = {Marcelo Gonzalez and Yannick Gueguen and Jean-Michel Escoubas and Jacques Dietrich and Julie Fievet and Franck Vandenbulcke and Evelyne Bachère},
year = {2003},
date = {2003-01-01},
booktitle = {Réunion Immunologie des Invertébrés. 17-19 novembre 2003, Banyuls},
note = {ACTN},
keywords = {ER4},
pubstate = {published},
tppubtype = {conference}
}
|
2003Conférence ER4 Auteurs : Lefebvre, Christophe; Cocquerelle, Claude; Vandenbulcke, Franck; Hot, David; Huot, Ludovic; Lemoine, Yves; Salzet, Michel Implication de la cystatine B et des cathepsines dans l'immunité innée chez la sangsue Theromyzon tessulatum Réunion Immunologie des Invertébrés. 17-19 novembre 2003, Banyuls, 2003, (COM). @conference{Lefebvre2003,
title = {Implication de la cystatine B et des cathepsines dans l'immunité innée chez la sangsue Theromyzon tessulatum},
author = {Christophe Lefebvre and Claude Cocquerelle and Franck Vandenbulcke and David Hot and Ludovic Huot and Yves Lemoine and Michel Salzet},
year = {2003},
date = {2003-01-01},
booktitle = {Réunion Immunologie des Invertébrés. 17-19 novembre 2003, Banyuls},
note = {COM},
keywords = {ER4},
pubstate = {published},
tppubtype = {conference}
}
|
2003Article de journal ER4 Auteurs : Munoz, Marcelo; Vandenbulcke, Franck; Gueguen, Yannick; Bachère, Evelyne Expression of penaeidin antimicrobial peptides in early larval stages of the shrimp Penaeus vannamei Dans: Developmental and Comparative Immunology, vol. 27, no. 4, p. 283-289, 2003, (ACL). @article{Munoz2003,
title = {Expression of penaeidin antimicrobial peptides in early larval stages of the shrimp Penaeus vannamei},
author = {Marcelo Munoz and Franck Vandenbulcke and Yannick Gueguen and Evelyne Bachère},
year = {2003},
date = {2003-01-01},
journal = {Developmental and Comparative Immunology},
volume = {27},
number = {4},
pages = {283-289},
note = {ACL},
keywords = {ER4},
pubstate = {published},
tppubtype = {article}
}
|
2003Conférence ER4 Auteurs : Roch, Philippe; Mitta, Guillaume; Vandenbulcke, Franck; Salzet, Michel; Aumelas, André; Yang, Yin-Shan; Chavanieu, Alain; Calas, Bernard Originality of the Mytilus (Bivalve Mollusc) antibacterial peptides: structurally related to insects but involved as in mammals The new panorama of animal evolution. Proceedings of the XVIII International Congress of Zoology, September 2000, Athens (Greece), Pensoft Publishers. Sofia-Moscow, 2003, (ACTI). @conference{Roch2003,
title = {Originality of the Mytilus (Bivalve Mollusc) antibacterial peptides: structurally related to insects but involved as in mammals},
author = {Philippe Roch and Guillaume Mitta and Franck Vandenbulcke and Michel Salzet and André Aumelas and Yin-Shan Yang and Alain Chavanieu and Bernard Calas},
year = {2003},
date = {2003-01-01},
booktitle = {The new panorama of animal evolution. Proceedings of the XVIII International Congress of Zoology, September 2000, Athens (Greece)},
pages = {177-184},
publisher = {Pensoft Publishers. Sofia-Moscow},
note = {ACTI},
keywords = {ER4},
pubstate = {published},
tppubtype = {conference}
}
|
2003Conférence ER4 Auteurs : Vergote, David; Vandenbulcke, Franck; Vieau, Didier; Salzet, Michel; Sautière, Pierre-Eric Le système nerveux de la sangsue répond il à une stimulation bactérienne? Réunion Immunologie des Invertébrés. 17-19 novembre 2003, Banyuls, 2003, (ACTN). @conference{Vergote2003,
title = {Le système nerveux de la sangsue répond il à une stimulation bactérienne?},
author = {David Vergote and Franck Vandenbulcke and Didier Vieau and Michel Salzet and Pierre-Eric Sautière},
year = {2003},
date = {2003-01-01},
booktitle = {Réunion Immunologie des Invertébrés. 17-19 novembre 2003, Banyuls},
note = {ACTN},
keywords = {ER4},
pubstate = {published},
tppubtype = {conference}
}
|
2002Ouvrage ER4 Auteurs : Beaudet, Alain; Jackson, Alexander-C.; Vandenbulcke, Franck Confocal and Electron Microscopic Tracking of Internalized Neuropeptides and/or Their Receptors 2002, (OS). Résumé @book{Beaudet2002,
title = {Confocal and Electron Microscopic Tracking of Internalized Neuropeptides and/or Their Receptors},
author = {Alain Beaudet and Alexander-C. Jackson and Franck Vandenbulcke},
year = {2002},
date = {2002-01-01},
booktitle = {Cellular and Molecular Methods in Neuroscience Research},
pages = {15-28},
abstract = {This chapter describes confocal and electron microscopic methods for tracking peptide ligands and/or their receptors following their internalization in cell cultures or brain slices. the confocal microscopic techniques are based upon the use of high affinity fluorescent ligands that were originally developed in our laboratory to study the fate of internalized neurotensin (nt), somatostatin (srif), and opioid peptides (9,13,17). the electron microscopic techniques are adapted from the pre-embedding immunogold method developed by virginia pickel and her team (5) as applied by us to study the effect of ligand exposure on the subcellular distribution of various subtypes of neuropeptide receptors.},
note = {OS},
keywords = {ER4},
pubstate = {published},
tppubtype = {book}
}
This chapter describes confocal and electron microscopic methods for tracking peptide ligands and/or their receptors following their internalization in cell cultures or brain slices. the confocal microscopic techniques are based upon the use of high affinity fluorescent ligands that were originally developed in our laboratory to study the fate of internalized neurotensin (nt), somatostatin (srif), and opioid peptides (9,13,17). the electron microscopic techniques are adapted from the pre-embedding immunogold method developed by virginia pickel and her team (5) as applied by us to study the effect of ligand exposure on the subcellular distribution of various subtypes of neuropeptide receptors. |
2002Article de journal ER4 Auteurs : Munoz, Marcelo; Vandenbulcke, Franck; Saulnier, Denis; Bachère, Evelyne Expression and distribution of penaeidin antimicrobial peptides are regulated by haemocyte reactions in microbial challenged shrimp Dans: European Journal of Biochemistry, vol. 269, no. 11, p. 2678-2689, 2002, (ACL). @article{Munoz2002,
title = {Expression and distribution of penaeidin antimicrobial peptides are regulated by haemocyte reactions in microbial challenged shrimp},
author = {Marcelo Munoz and Franck Vandenbulcke and Denis Saulnier and Evelyne Bachère},
year = {2002},
date = {2002-01-01},
journal = {European Journal of Biochemistry},
volume = {269},
number = {11},
pages = {2678-2689},
note = {ACL},
keywords = {ER4},
pubstate = {published},
tppubtype = {article}
}
|
2002Ouvrage ER4 Auteurs : Sautière, Pierre-Eric; Tasiemski, Aurélie; Vandenbulcke, Franck; Desmons, Annie; Salzet, Michel New powerful cascade coagulant inhibitors isolated from the gut of the leech Theromyzon tessulatum E.L. Cooper et al. (Eds) IOS Press, 2002, (OS). @book{Sautiere2002,
title = {New powerful cascade coagulant inhibitors isolated from the gut of the leech Theromyzon tessulatum},
author = {Pierre-Eric Sautière and Aurélie Tasiemski and Franck Vandenbulcke and Annie Desmons and Michel Salzet},
year = {2002},
date = {2002-01-01},
booktitle = {A new model for analyzing antimicrobial peptides with biomedical applications},
edition = {E.L. Cooper et al. (Eds) IOS Press},
note = {OS},
keywords = {ER4},
pubstate = {published},
tppubtype = {book}
}
|
2002Article de journal ER4 Auteurs : Tasiemski, Aurélie; Hammad, Hamida; Vandenbulcke, Franck; Breton, Christophe; Bilfinger, Thomas-J.; Pestel, Joel; Salzet, Michel Presence of chromogranin-derived antimicrobial peptides in plasma during coronary artery bypass surgery and evidence of an immune origin of these peptides Dans: Blood, vol. 100, no. 2, p. 553-559, 2002, (ACL). @article{Tasiemski2002,
title = {Presence of chromogranin-derived antimicrobial peptides in plasma during coronary artery bypass surgery and evidence of an immune origin of these peptides},
author = {Aurélie Tasiemski and Hamida Hammad and Franck Vandenbulcke and Christophe Breton and Thomas-J. Bilfinger and Joel Pestel and Michel Salzet},
year = {2002},
date = {2002-01-01},
journal = {Blood},
volume = {100},
number = {2},
pages = {553-559},
note = {ACL},
keywords = {ER4},
pubstate = {published},
tppubtype = {article}
}
|
2002Conférence ER4 Auteurs : Vandenbulcke, Franck; Grelle, Claire; Fabre, Marie-Chantal; Descamps, Michel Autometallography: a cytological method for intracellular localization of heavy metals Advantages and limits. Joint Microscopy Meeting. 25-28 juin 2002, Lille, 2002, (ACTI). @conference{Vandenbulcke2002,
title = {Autometallography: a cytological method for intracellular localization of heavy metals},
author = {Franck Vandenbulcke and Claire Grelle and Marie-Chantal Fabre and Michel Descamps},
year = {2002},
date = {2002-01-01},
booktitle = {Advantages and limits. Joint Microscopy Meeting. 25-28 juin 2002, Lille},
note = {ACTI},
keywords = {ER4},
pubstate = {published},
tppubtype = {conference}
}
|
2002Conférence ER4 Auteurs : Vandenbulcke, Franck; Tasiemski, Aurélie; Finet, H; Desmons, Annie; Vizioli, Jacopo; Sautière, Pierre-Eric; Salzet, Michel Caractérisation et rôle des peptides antimicrobiens dans l’immunité innée de la sangsue Theromyzon tessulatum 126ème Congrés de la Société Zoologique de France. 16-18 septembre 2002, Plouzané, 2002, (COM). @conference{Vandenbulcke2002a,
title = {Caractérisation et rôle des peptides antimicrobiens dans l’immunité innée de la sangsue Theromyzon tessulatum},
author = {Franck Vandenbulcke and Aurélie Tasiemski and H Finet and Annie Desmons and Jacopo Vizioli and Pierre-Eric Sautière and Michel Salzet},
year = {2002},
date = {2002-01-01},
booktitle = {126ème Congrés de la Société Zoologique de France. 16-18 septembre 2002, Plouzané},
note = {COM},
keywords = {ER4},
pubstate = {published},
tppubtype = {conference}
}
|
2001Conférence ER4 Auteurs : Deloffre, Laurence; Sautière, Pierre-Eric; Vieau, Didier; Vandenbulcke, Franck; Salzet, Michel; Andriès, Jean-Claude Caractérisation moléculaire d’une molécule apparentée à la progranuline, chez Hediste diversicolor Colloque « Immunologie des Invertébrés ». 11-14 Septembre 2001, Villeneuve d’Ascq, 2001, (ACTN). @conference{Deloffre2001,
title = {Caractérisation moléculaire d’une molécule apparentée à la progranuline, chez Hediste diversicolor},
author = {Laurence Deloffre and Pierre-Eric Sautière and Didier Vieau and Franck Vandenbulcke and Michel Salzet and Jean-Claude Andriès},
year = {2001},
date = {2001-01-01},
booktitle = {Colloque « Immunologie des Invertébrés ». 11-14 Septembre 2001, Villeneuve d’Ascq},
note = {ACTN},
keywords = {ER4},
pubstate = {published},
tppubtype = {conference}
}
|
2001Article de journal ER4 Auteurs : Matias, Isabel; Bisogno, Tiziana; Melck, Dominique; Vandenbulcke, Franck; Verger-Bocquet, Martine; De-Petrocellis, Luciano; Sergheraert, Christian; Breton, Christophe; Di-Marzo, Vicenzo; Salzet, Michel Evidence for an endocannabinoid system in the central nervous system of the leech Hirudo medicinalis Dans: Molecular Brain Research, vol. 87, no. 2, p. 145-159, 2001, (ACL). Résumé @article{Matias2001,
title = {Evidence for an endocannabinoid system in the central nervous system of the leech Hirudo medicinalis},
author = {Isabel Matias and Tiziana Bisogno and Dominique Melck and Franck Vandenbulcke and Martine Verger-Bocquet and Luciano De-Petrocellis and Christian Sergheraert and Christophe Breton and Vicenzo Di-Marzo and Michel Salzet},
year = {2001},
date = {2001-01-01},
journal = {Molecular Brain Research},
volume = {87},
number = {2},
pages = {145-159},
abstract = {In invertebrates, like hydra and sea urchins, evidence for a functional cannabinoid system was described. the partial characterization of a putative cb1 cannabinoid receptor in the leech hirudo medicinalis led us to investigate the presence of a complete endogenous cannabinoid system in this organism. by using gas chromatography–mass spectrometry, we demonstrate the presence of the endocannabinoids anandamide (n-arachidonoylethanolamine, 21.560.7 pmol/g) and 2-arachidonoyl-glycerol (147.4642.7 pmol/g), and of the biosynthetic precursor of anandamide, n-arachidonylphosphatidyl-ethanolamine (16.563.3 pmol/g), in the leech central nervous system (cns). anandamide-related molecules such as n-palmitoylethanolamine (32.461.6 pmol/g) and n-linolenoylethanolamine (5.8 pmol/g) were also detected.we also found an anandamide amidase activity in the leech cns cytosolic fraction with a maximal activity at ph 7 and little sensitivity to typical fatty acid amide hydrolase (faah) inhibitors. using an antiserum directed against the amidase signature sequence, we focused on the identification and the localization of the leech amidase. firstly, leech nervous system protein extract was subjected to western blot analysis, which showed three immunoreactive bands at ca. |42, |46 and |66 kda. the former and latter bands were very faint and were also detected in whole homogenates from the coelenterate hydra vulgaris, where the presence of cb1-like receptors, endocannabinoids and a faah-like activity was reported previously. secondly, amidase immunocytochemical detection revealed numerous immunoreactive neurons in the cns of three species of leeches. in addition, we observed that leech amidase-like immunoreactivity matches to a certain extent with cb1-like immunoreactivity. finally, we also found that stimulation by anandamide of this receptor leads, as in mammals, to inhibition of camp formation, although this effect appeared to be occurring through the previously described anandamide-induced and cb1-mediated activation of nitric oxide release. taken together, these results suggest the existence of a complete and functional cannabinoid system in leeches. ó 2001 elsevier science b.v. all rights reserved.},
note = {ACL},
keywords = {ER4},
pubstate = {published},
tppubtype = {article}
}
In invertebrates, like hydra and sea urchins, evidence for a functional cannabinoid system was described. the partial characterization of a putative cb1 cannabinoid receptor in the leech hirudo medicinalis led us to investigate the presence of a complete endogenous cannabinoid system in this organism. by using gas chromatography–mass spectrometry, we demonstrate the presence of the endocannabinoids anandamide (n-arachidonoylethanolamine, 21.560.7 pmol/g) and 2-arachidonoyl-glycerol (147.4642.7 pmol/g), and of the biosynthetic precursor of anandamide, n-arachidonylphosphatidyl-ethanolamine (16.563.3 pmol/g), in the leech central nervous system (cns). anandamide-related molecules such as n-palmitoylethanolamine (32.461.6 pmol/g) and n-linolenoylethanolamine (5.8 pmol/g) were also detected.we also found an anandamide amidase activity in the leech cns cytosolic fraction with a maximal activity at ph 7 and little sensitivity to typical fatty acid amide hydrolase (faah) inhibitors. using an antiserum directed against the amidase signature sequence, we focused on the identification and the localization of the leech amidase. firstly, leech nervous system protein extract was subjected to western blot analysis, which showed three immunoreactive bands at ca. |42, |46 and |66 kda. the former and latter bands were very faint and were also detected in whole homogenates from the coelenterate hydra vulgaris, where the presence of cb1-like receptors, endocannabinoids and a faah-like activity was reported previously. secondly, amidase immunocytochemical detection revealed numerous immunoreactive neurons in the cns of three species of leeches. in addition, we observed that leech amidase-like immunoreactivity matches to a certain extent with cb1-like immunoreactivity. finally, we also found that stimulation by anandamide of this receptor leads, as in mammals, to inhibition of camp formation, although this effect appeared to be occurring through the previously described anandamide-induced and cb1-mediated activation of nitric oxide release. taken together, these results suggest the existence of a complete and functional cannabinoid system in leeches. ó 2001 elsevier science b.v. all rights reserved. |
2001Conférence ER4 Auteurs : Munoz, Marcelo; Vandenbulcke, Franck; Saulnier, Denis; Bachère, Evelyne Les pénaeidines dans la réponse immunitaire chez les crevettes pénéides Colloque « Immunologie des Invertébrés ». 11-14 Septembre 2001, Villeneuve d’Ascq, 2001, (COM). @conference{Munoz2001,
title = {Les pénaeidines dans la réponse immunitaire chez les crevettes pénéides},
author = {Marcelo Munoz and Franck Vandenbulcke and Denis Saulnier and Evelyne Bachère},
year = {2001},
date = {2001-01-01},
booktitle = {Colloque « Immunologie des Invertébrés ». 11-14 Septembre 2001, Villeneuve d’Ascq},
note = {COM},
keywords = {ER4},
pubstate = {published},
tppubtype = {conference}
}
|
2001Conférence ER4 Auteurs : Rivière, Guillaume; Deloffre, Laurence; Vandenbulcke, Franck; Levoye, Angélique; Breton, Christophe; Salzet, Michel; Vieau, Didier Clonage et étude du mode d’expression de l’enzyme de conversion de l’angiotensine chez la sangsue Theromyzon tessulatum Colloque du réseau LARC (Lille-Amiens-Rouen-Caen). 15 octobre 2001, Rouen, 2001, (ACTN). @conference{Riviere2001,
title = {Clonage et étude du mode d’expression de l’enzyme de conversion de l’angiotensine chez la sangsue Theromyzon tessulatum},
author = {Guillaume Rivière and Laurence Deloffre and Franck Vandenbulcke and Angélique Levoye and Christophe Breton and Michel Salzet and Didier Vieau},
year = {2001},
date = {2001-01-01},
booktitle = {Colloque du réseau LARC (Lille-Amiens-Rouen-Caen). 15 octobre 2001, Rouen},
note = {ACTN},
keywords = {ER4},
pubstate = {published},
tppubtype = {conference}
}
|
2001Conférence ER4 Auteurs : Tasiemski, Aurélie; Vandenbulcke, Franck; Mitta, Guillaume; Sautière, Pierre-Eric; Lemoine, Jérôme; Salzet, Michel Gene expression of theromacin, a novel cystein rich antibacterial peptide isolated from the leech Theromyzon tessulatum, is induced after bacterial challenge Colloque « Immunologie des Invertébrés ». 11-14 septembre 2001, Villeneuve d’Ascq, 2001, (ACTN). @conference{Tasiemski2001,
title = {Gene expression of theromacin, a novel cystein rich antibacterial peptide isolated from the leech Theromyzon tessulatum, is induced after bacterial challenge},
author = {Aurélie Tasiemski and Franck Vandenbulcke and Guillaume Mitta and Pierre-Eric Sautière and Jérôme Lemoine and Michel Salzet},
year = {2001},
date = {2001-01-01},
booktitle = {Colloque « Immunologie des Invertébrés ». 11-14 septembre 2001, Villeneuve d’Ascq},
note = {ACTN},
keywords = {ER4},
pubstate = {published},
tppubtype = {conference}
}
|
2000Article de journal ER4 Auteurs : Chopin, Vincent; Salzet, Michel; Baert, Jean-Luc; Vandenbulcke, Franck; Sautière, Pierre-Eric; Kerckaert, Jean-Pierre; Malecha, Jean Therostasin, a novel clotting factor Xa inhibitor from the rhynchobdellid leech, Theromyzon tessulatum Dans: Journal of Biological Chemistry, vol. 275, no. 42, p. 32701-32707, 2000, (ACL). Résumé @article{Chopin2000,
title = {Therostasin, a novel clotting factor Xa inhibitor from the rhynchobdellid leech, Theromyzon tessulatum},
author = {Vincent Chopin and Michel Salzet and Jean-Luc Baert and Franck Vandenbulcke and Pierre-Eric Sautière and Jean-Pierre Kerckaert and Jean Malecha},
year = {2000},
date = {2000-01-01},
journal = {Journal of Biological Chemistry},
volume = {275},
number = {42},
pages = {32701-32707},
abstract = {Therostasin is a potent naturally occurring tight-binding inhibitor of mammalian factor xa (k i, 34 pm), isolated from the rhynchobdellid leechtheromyzon tessulatum. therostasin is a cysteine-rich protein (8991 da) consisting of 82 amino acid residues with 16 cysteine residues. its amino acid sequence has been determined by a combination of techniques, including edman degradation, enzymatic cleavage, and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof ms) on the native and s-β-pyridylethylated compound. sequence analysis reveals that it shares no significant homology with other factor xa inhibitors except for the putative reactive site. moreover, it contains a signature pattern for proteins of the endothelin family, potent vasoconstrictors isolated in mammal and snake venom. therostasin cdna (825 bp) codes for a polypeptide of 82 amino acid residues preceded by 19 residues, representing a signal peptide sequence. as for the other known inhibitors of factor xa, therostasin is expressed and stored in the cells of the leech salivary glands.},
note = {ACL},
keywords = {ER4},
pubstate = {published},
tppubtype = {article}
}
Therostasin is a potent naturally occurring tight-binding inhibitor of mammalian factor xa (k i, 34 pm), isolated from the rhynchobdellid leechtheromyzon tessulatum. therostasin is a cysteine-rich protein (8991 da) consisting of 82 amino acid residues with 16 cysteine residues. its amino acid sequence has been determined by a combination of techniques, including edman degradation, enzymatic cleavage, and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof ms) on the native and s-β-pyridylethylated compound. sequence analysis reveals that it shares no significant homology with other factor xa inhibitors except for the putative reactive site. moreover, it contains a signature pattern for proteins of the endothelin family, potent vasoconstrictors isolated in mammal and snake venom. therostasin cdna (825 bp) codes for a polypeptide of 82 amino acid residues preceded by 19 residues, representing a signal peptide sequence. as for the other known inhibitors of factor xa, therostasin is expressed and stored in the cells of the leech salivary glands. |
2000Conférence ER4 Auteurs : Matias, Isabel; Bisogno, Tiziana; Melck, Dominique; Vandenbulcke, Franck; Breton, Christophe; Di-Marzo, Vicenzo; Salzet, Michel Evidence for a cannabinoid system in leech Hirudo medicinalis International Symposium on non-mammalian eicosanoids and bioactive lipids. 29-31 May 2000, Berlin (Allemagne), 2000, (ACTI). @conference{Matias2000,
title = {Evidence for a cannabinoid system in leech Hirudo medicinalis},
author = {Isabel Matias and Tiziana Bisogno and Dominique Melck and Franck Vandenbulcke and Christophe Breton and Vicenzo Di-Marzo and Michel Salzet},
year = {2000},
date = {2000-01-01},
booktitle = {International Symposium on non-mammalian eicosanoids and bioactive lipids. 29-31 May 2000, Berlin (Allemagne)},
note = {ACTI},
keywords = {ER4},
pubstate = {published},
tppubtype = {conference}
}
|
2000Article de journal ER4 Auteurs : Mitta, Guillaume; Vandenbulcke, Franck; Hubert, Florence; Salzet, Michel; Roch, Philippe Involvement of mytilins in mussel antimicrobial defense Dans: Journal of Biological Chemistry, vol. 275, no. 17, p. 12954-12962, 2000, (ACL). @article{Mitta2000,
title = {Involvement of mytilins in mussel antimicrobial defense},
author = {Guillaume Mitta and Franck Vandenbulcke and Florence Hubert and Michel Salzet and Philippe Roch},
year = {2000},
date = {2000-01-01},
journal = {Journal of Biological Chemistry},
volume = {275},
number = {17},
pages = {12954-12962},
note = {ACL},
keywords = {ER4},
pubstate = {published},
tppubtype = {article}
}
|
2000Article de journal ER4 Auteurs : Mitta, Guillaume; Vandenbulcke, Franck; Noël, Thierry; Romestand, Bernard; Beauvillain, Jean-Claude; Salzet, Michel; Roch, Philippe Differential distribution and defence involvement of antimicrobial peptides in mussel Dans: Journal of Cell Science, vol. 113, no. 15, p. 2759-2769, 2000, (ACL). @article{Mitta2000a,
title = {Differential distribution and defence involvement of antimicrobial peptides in mussel},
author = {Guillaume Mitta and Franck Vandenbulcke and Thierry Noël and Bernard Romestand and Jean-Claude Beauvillain and Michel Salzet and Philippe Roch},
year = {2000},
date = {2000-01-01},
journal = {Journal of Cell Science},
volume = {113},
number = {15},
pages = {2759-2769},
note = {ACL},
keywords = {ER4},
pubstate = {published},
tppubtype = {article}
}
|
2000Article de journal ER4 Auteurs : Mitta, Guillaume; Vandenbulcke, Franck; Roch, Philippe Original involvement of antimicrobial peptides in mussel innate immunity Dans: FEBS Letters, vol. 486, no. 3, p. 185-190, 2000, (ACL). @article{Mitta2000b,
title = {Original involvement of antimicrobial peptides in mussel innate immunity},
author = {Guillaume Mitta and Franck Vandenbulcke and Philippe Roch},
year = {2000},
date = {2000-01-01},
journal = {FEBS Letters},
volume = {486},
number = {3},
pages = {185-190},
note = {ACL},
keywords = {ER4},
pubstate = {published},
tppubtype = {article}
}
|
2000Article de journal ER4 Auteurs : Vandenbulcke, Franck; Nouel, Dominique; Vincent, Jean-Pierre; Mazella, Jean; Beaudet, Alain Ligand-induced internalization of neurotensin in transfected COS-7 cells: Differential intracellular trafficking of ligand and receptor Dans: Journal of Cell Science, vol. 113, no. 17, p. 2963-2975, 2000, (ACL). Résumé @article{Vandenbulcke2000,
title = {Ligand-induced internalization of neurotensin in transfected COS-7 cells: Differential intracellular trafficking of ligand and receptor},
author = {Franck Vandenbulcke and Dominique Nouel and Jean-Pierre Vincent and Jean Mazella and Alain Beaudet},
year = {2000},
date = {2000-01-01},
journal = {Journal of Cell Science},
volume = {113},
number = {17},
pages = {2963-2975},
abstract = {The neuropeptide neurotensin (nt) is known to be internalized in a receptor-mediated fashion into its target cells. to gain insight into the mechanisms underlying this process, we monitored in parallel the migration of the nt1 neurotensin receptor subtype and a fluorescent analog of nt (fluo-nt) in cos-7 cells transfected with a tagged nt1 construct. fluo-nt internalization was prevented by hypertonic sucrose, potassium depletion and cytosol acidification, demonstrating that it proceeded via clathrin-coated pits. within 0–30 minutes, fluo-nt accumulated together with its receptor in acridine orange-positive, acidic organelles. these organelles concentrated transferrin and immunostained positively for rab 5a, therefore they were early endosomes. after 30–45 minutes, the ligand and its receptor no longer colocalized. fluo-nt was first found in rab 7-positive late endosomes and later in a nonacidic juxtanuclear compartment identified as the trans-golgi network (tgn) by virtue of its staining for syntaxin 6. this juxtanuclear compartment also stained positively for rab 7 and for the tgn/pericentriolar recycling endosome marker rab 11, suggesting that the ligand could have been recruited to the tgn from either late or recycling endosomes. by that time, internalized receptors were detected in lamp-1-immunoreactive lysosomes. these results demonstrate that neurotensin/nt1 receptor complexes follow a recycling cycle that is unique among the g protein-coupled receptors studied to date, and provide the first evidence for the targeting of a nonendogenous protein from endosomes to the tgn},
note = {ACL},
keywords = {ER4},
pubstate = {published},
tppubtype = {article}
}
The neuropeptide neurotensin (nt) is known to be internalized in a receptor-mediated fashion into its target cells. to gain insight into the mechanisms underlying this process, we monitored in parallel the migration of the nt1 neurotensin receptor subtype and a fluorescent analog of nt (fluo-nt) in cos-7 cells transfected with a tagged nt1 construct. fluo-nt internalization was prevented by hypertonic sucrose, potassium depletion and cytosol acidification, demonstrating that it proceeded via clathrin-coated pits. within 0–30 minutes, fluo-nt accumulated together with its receptor in acridine orange-positive, acidic organelles. these organelles concentrated transferrin and immunostained positively for rab 5a, therefore they were early endosomes. after 30–45 minutes, the ligand and its receptor no longer colocalized. fluo-nt was first found in rab 7-positive late endosomes and later in a nonacidic juxtanuclear compartment identified as the trans-golgi network (tgn) by virtue of its staining for syntaxin 6. this juxtanuclear compartment also stained positively for rab 7 and for the tgn/pericentriolar recycling endosome marker rab 11, suggesting that the ligand could have been recruited to the tgn from either late or recycling endosomes. by that time, internalized receptors were detected in lamp-1-immunoreactive lysosomes. these results demonstrate that neurotensin/nt1 receptor complexes follow a recycling cycle that is unique among the g protein-coupled receptors studied to date, and provide the first evidence for the targeting of a nonendogenous protein from endosomes to the tgn |
1999Conférence ER4 Auteurs : Deram, Annabelle; Grelle, Claire; Vandenbulcke, Franck; Fabre, Marie-Chantal; Scaps, Patrick; Descamps, Michel Lead biodisponibility to soil invertebrate fauna and perennial grass International Conference on lead exposure, Reproductive toxixity and carcinogenicity. 7-9 June 1999, Gargano (Italie), 1999, (ACTI). @conference{Deram1999,
title = {Lead biodisponibility to soil invertebrate fauna and perennial grass},
author = {Annabelle Deram and Claire Grelle and Franck Vandenbulcke and Marie-Chantal Fabre and Patrick Scaps and Michel Descamps},
year = {1999},
date = {1999-01-01},
booktitle = {International Conference on lead exposure, Reproductive toxixity and carcinogenicity. 7-9 June 1999, Gargano (Italie)},
note = {ACTI},
keywords = {ER4},
pubstate = {published},
tppubtype = {conference}
}
|
1999Article de journal ER4 Auteurs : Garrido, Paula-A. G.; Vandenbulcke, Franck; Ramjaun, Antoine-R.; Vincent, Bruno; Checler, Frédéric; Ferro, Emer; Beaudet, Alain Confocal microscopy reveals thimet oligopeptidase (EC 3.4.24.15) and neurolysin (EC 3.4.24.16) in the classical secretory pathway Dans: DNA and Cell Biology, vol. 18, no. 4, p. 323-331, 1999, (ACL). Résumé @article{Garrido1999,
title = {Confocal microscopy reveals thimet oligopeptidase (EC 3.4.24.15) and neurolysin (EC 3.4.24.16) in the classical secretory pathway},
author = {Paula-A.G. Garrido and Franck Vandenbulcke and Antoine-R. Ramjaun and Bruno Vincent and Frédéric Checler and Emer Ferro and Alain Beaudet},
year = {1999},
date = {1999-01-01},
journal = {DNA and Cell Biology},
volume = {18},
number = {4},
pages = {323-331},
abstract = {Thimet oligopeptidase (ec 3.4.24.15; ep24.15) and neurolysin (ec 3.4.24.16; ep24.16) are closely related enzymes involved in the metabolic inactivation of bioactive peptides. both of these enzymes were previously shown to be secreted from a variety of cell types, although their primary sequence lacks a signal peptide. to investigate the mechanisms responsible for this secretion, we examined by confocal microscopy the subcellular localization of these two enzymes in the neuroendocrine cell line att20. both ep24.15 and ep24.16 were found by immunohistochemistry to be abundantly expressed in att20 cells. western blotting experiments confirmed that the immunoreactivity detected in the soma of these cells corresponded to previously cloned isoforms of the enzymes. at the subcellular level, both enzymes colocalized extensively with the integral trans-golgi network protein, syntaxin-6, in the juxtanuclear region. in addition, both ep24.15 and ep24.16 were found within small vesicular organelles distributed throughout the cell body. some, but not all, of these organelles also stained positively for acth. these results demonstrate that both ep24.15 and ep24.16 are present within the classical secretory pathway. their colocalization with acth further suggests that they may be targeted to the regulated secretory pathway, even in the absence of a signal peptide.},
note = {ACL},
keywords = {ER4},
pubstate = {published},
tppubtype = {article}
}
Thimet oligopeptidase (ec 3.4.24.15; ep24.15) and neurolysin (ec 3.4.24.16; ep24.16) are closely related enzymes involved in the metabolic inactivation of bioactive peptides. both of these enzymes were previously shown to be secreted from a variety of cell types, although their primary sequence lacks a signal peptide. to investigate the mechanisms responsible for this secretion, we examined by confocal microscopy the subcellular localization of these two enzymes in the neuroendocrine cell line att20. both ep24.15 and ep24.16 were found by immunohistochemistry to be abundantly expressed in att20 cells. western blotting experiments confirmed that the immunoreactivity detected in the soma of these cells corresponded to previously cloned isoforms of the enzymes. at the subcellular level, both enzymes colocalized extensively with the integral trans-golgi network protein, syntaxin-6, in the juxtanuclear region. in addition, both ep24.15 and ep24.16 were found within small vesicular organelles distributed throughout the cell body. some, but not all, of these organelles also stained positively for acth. these results demonstrate that both ep24.15 and ep24.16 are present within the classical secretory pathway. their colocalization with acth further suggests that they may be targeted to the regulated secretory pathway, even in the absence of a signal peptide. |
1999Conférence ER4 Auteurs : Grelle, Claire; Fabre, Marie-Chantal; Leprêtre, Alain; Vandenbulcke, Franck; Descamps, Michel L’impact des métaux lourds sur les peuplements de macroinvertébrés de la faune du sol Colloque « Les enjeux, pour la santé humaine et le maintien de la biodiversité, de la caractérisation et de la validation de systèmes d’évaluation écotoxicologiques de la qualité des sédiments et des boues de dragage. MACC. 28 septembre 1999, Villeneuve d’Ascq, 1999, (ACTN). @conference{Grelle1999,
title = {L’impact des métaux lourds sur les peuplements de macroinvertébrés de la faune du sol},
author = {Claire Grelle and Marie-Chantal Fabre and Alain Leprêtre and Franck Vandenbulcke and Michel Descamps},
year = {1999},
date = {1999-01-01},
booktitle = {Colloque « Les enjeux, pour la santé humaine et le maintien de la biodiversité, de la caractérisation et de la validation de systèmes d’évaluation écotoxicologiques de la qualité des sédiments et des boues de dragage. MACC. 28 septembre 1999, Villeneuve d’Ascq},
note = {ACTN},
keywords = {ER4},
pubstate = {published},
tppubtype = {conference}
}
|
1999Conférence ER4 Auteurs : Matias, Isabel; Breton, Christophe; Vandenbulcke, Franck; Verger, Martine; Salzet, Michel L’anandamide amidase chez les Hirudinées Journée Scientifique du réseau LARC-Neurosciences. 3 octobre 1999, Amiens, 1999, (ACTN). @conference{Matias1999,
title = {L’anandamide amidase chez les Hirudinées},
author = {Isabel Matias and Christophe Breton and Franck Vandenbulcke and Martine Verger and Michel Salzet},
year = {1999},
date = {1999-01-01},
booktitle = {Journée Scientifique du réseau LARC-Neurosciences. 3 octobre 1999, Amiens},
note = {ACTN},
keywords = {ER4},
pubstate = {published},
tppubtype = {conference}
}
|
1999Conférence ER4 Auteurs : Matias, Isabel; Breton, Christophe; Vandenbulcke, Franck; Verger, Martine; Salzet, Michel L’anandamide amidase chez les Hirudinées Réunion « Immunologie de Invertébrés ». 19-22 septembre 1999, Mont Sainte-Odile, 1999, (ACTN). @conference{Matias1999a,
title = {L’anandamide amidase chez les Hirudinées},
author = {Isabel Matias and Christophe Breton and Franck Vandenbulcke and Martine Verger and Michel Salzet},
year = {1999},
date = {1999-01-01},
booktitle = {Réunion « Immunologie de Invertébrés ». 19-22 septembre 1999, Mont Sainte-Odile},
note = {ACTN},
keywords = {ER4},
pubstate = {published},
tppubtype = {conference}
}
|
1999Article de journal ER4 Auteurs : Mitta, Guillaume; Vandenbulcke, Franck; Hubert, Florence; Roch, Philippe Mussel defensins are synthesised and processed in granulocytes then released into the plasma after bacterial challenge Dans: Journal of Cell Science, vol. 112, no. 23, p. 4233-4242, 1999, (ACL). @article{Mitta1999,
title = {Mussel defensins are synthesised and processed in granulocytes then released into the plasma after bacterial challenge},
author = {Guillaume Mitta and Franck Vandenbulcke and Florence Hubert and Philippe Roch},
year = {1999},
date = {1999-01-01},
journal = {Journal of Cell Science},
volume = {112},
number = {23},
pages = {4233-4242},
note = {ACL},
keywords = {ER4},
pubstate = {published},
tppubtype = {article}
}
|
1999Conférence ER4 Auteurs : Vandenbulcke, Franck; Mitta, Guillaume; Salzet, Michel; Roch, Philippe Les peptides antimicrobiens chez Mytilus galloprovincialis Réunion « Immunologie de Invertébrés ». 19-22 septembre 1999, Mont Sainte-Odile, 1999, (COM). @conference{Vandenbulcke1999,
title = {Les peptides antimicrobiens chez Mytilus galloprovincialis},
author = {Franck Vandenbulcke and Guillaume Mitta and Michel Salzet and Philippe Roch},
year = {1999},
date = {1999-01-01},
booktitle = {Réunion « Immunologie de Invertébrés ». 19-22 septembre 1999, Mont Sainte-Odile},
note = {COM},
keywords = {ER4},
pubstate = {published},
tppubtype = {conference}
}
|
1999Conférence ER4 Auteurs : Vandenbulcke, Franck; Mitta, Guillaume; Salzet, Michel; Roch, Philippe Localisation ultrastructurale des peptides antimicrobiens Mytilin et MGD1 chez Mytilus Journée Scientifique du réseau LARC-Neurosciences. 3 octobre 1999, Amiens, 1999, (ACTN). @conference{Vandenbulcke1999a,
title = {Localisation ultrastructurale des peptides antimicrobiens Mytilin et MGD1 chez Mytilus},
author = {Franck Vandenbulcke and Guillaume Mitta and Michel Salzet and Philippe Roch},
year = {1999},
date = {1999-01-01},
booktitle = {Journée Scientifique du réseau LARC-Neurosciences. 3 octobre 1999, Amiens},
note = {ACTN},
keywords = {ER4},
pubstate = {published},
tppubtype = {conference}
}
|
1998Article de journal ER4 Auteurs : Beaudet, Alain; Nouel, Dominique; Stroh, Thomas; Vandenbulcke, Franck; Dal-Farra, C; Vincent, Jean-Pierre Fluorescent ligands for studying neuropeptide receptors by confocal microscopy Dans: Brazilian Journal of Medical and Biological Research, vol. 31, no. 11, p. 1479-1489, 1998, (ACL). @article{Beaudet1998,
title = {Fluorescent ligands for studying neuropeptide receptors by confocal microscopy},
author = {Alain Beaudet and Dominique Nouel and Thomas Stroh and Franck Vandenbulcke and C Dal-Farra and Jean-Pierre Vincent},
year = {1998},
date = {1998-01-01},
journal = {Brazilian Journal of Medical and Biological Research},
volume = {31},
number = {11},
pages = {1479-1489},
note = {ACL},
keywords = {ER4},
pubstate = {published},
tppubtype = {article}
}
|
1998Conférence ER4 Auteurs : Garrido, Paula-A. G.; Vandenbulcke, Franck; Ferro, Emer-Suavinho; Beaudet, Alain Metalloendopeptidase EC 3.4.24.15 is targeted to and secreted from the regulated secretory pathway in att-20 cells Congrès de la Fédération des Sociétés de Biologie Expérimentale. 26-29 August 1998, Caxambu (Brésil), 1998, (ACTI). @conference{Garrido1998,
title = {Metalloendopeptidase EC 3.4.24.15 is targeted to and secreted from the regulated secretory pathway in att-20 cells},
author = {Paula-A.G. Garrido and Franck Vandenbulcke and Emer-Suavinho Ferro and Alain Beaudet},
year = {1998},
date = {1998-01-01},
booktitle = {Congrès de la Fédération des Sociétés de Biologie Expérimentale. 26-29 August 1998, Caxambu (Brésil)},
note = {ACTI},
keywords = {ER4},
pubstate = {published},
tppubtype = {conference}
}
|
1998Article de journal ER4 Auteurs : Valentijn, Karine; Vandenbulcke, Franck; Piek, Ester; Beauvillain, Jean-Claude; Vaudry, Hubert Distribution, cellular localization, and ontogeny of preprothyrotropin- releasing hormone-(160-169) (Ps4)-binding sites in the rat pituitary Dans: Endocrinology, vol. 139, no. 3, p. 1306-1313, 1998, (ACL). @article{Valentijn1998,
title = {Distribution, cellular localization, and ontogeny of preprothyrotropin- releasing hormone-(160-169) (Ps4)-binding sites in the rat pituitary},
author = {Karine Valentijn and Franck Vandenbulcke and Ester Piek and Jean-Claude Beauvillain and Hubert Vaudry},
year = {1998},
date = {1998-01-01},
journal = {Endocrinology},
volume = {139},
number = {3},
pages = {1306-1313},
note = {ACL},
keywords = {ER4},
pubstate = {published},
tppubtype = {article}
}
|
1998Article de journal ER4 Auteurs : Vandenbulcke, Franck; Grelle, Claire; Fabre, Marie-Chantal; Descamps, Michel Implication of the midgut of the centipede Lithobius forficatus in the heavy metal detoxification process Dans: Ecotoxicology and Environmental Safety, vol. 41, no. 3, p. 258-268, 1998, (ACL). @article{Vandenbulcke1998,
title = {Implication of the midgut of the centipede Lithobius forficatus in the heavy metal detoxification process},
author = {Franck Vandenbulcke and Claire Grelle and Marie-Chantal Fabre and Michel Descamps},
year = {1998},
date = {1998-01-01},
journal = {Ecotoxicology and Environmental Safety},
volume = {41},
number = {3},
pages = {258-268},
note = {ACL},
keywords = {ER4},
pubstate = {published},
tppubtype = {article}
}
|
1998Article de journal ER4 Auteurs : Vandenbulcke, Franck; Grelle, Claire; Fabre, Marie-Chantal; Descamps, Michel Ultrastructural and autometallographic studies of the nephrocytes of Lithobius forficatus L. (Myriapoda, Chilopoda): Role in detoxification of cadmium and lead Dans: International Journal of Insect Morphology and Embryology, vol. 27, no. 2, p. 111-120, 1998, (ACL). @article{Vandenbulcke1998a,
title = {Ultrastructural and autometallographic studies of the nephrocytes of Lithobius forficatus L. (Myriapoda, Chilopoda): Role in detoxification of cadmium and lead},
author = {Franck Vandenbulcke and Claire Grelle and Marie-Chantal Fabre and Michel Descamps},
year = {1998},
date = {1998-01-01},
journal = {International Journal of Insect Morphology and Embryology},
volume = {27},
number = {2},
pages = {111-120},
note = {ACL},
keywords = {ER4},
pubstate = {published},
tppubtype = {article}
}
|
1998Conférence ER4 Auteurs : Vandenbulcke, Franck; Nouel, Dominique; Vincent, Jean-Pierre; Beaudet, Alain Cheminement intracellulaire differentiel de la neurotensine et de son récepteur après internalisation dans des cellules COS-7 transfectées XXVII colloque de la Société de Neuroendocrinologie Expérimentale. 2-5 septembre 1998, Lille, 1998, (COM). @conference{Vandenbulcke1998b,
title = {Cheminement intracellulaire differentiel de la neurotensine et de son récepteur après internalisation dans des cellules COS-7 transfectées},
author = {Franck Vandenbulcke and Dominique Nouel and Jean-Pierre Vincent and Alain Beaudet},
year = {1998},
date = {1998-01-01},
booktitle = {XXVII colloque de la Société de Neuroendocrinologie Expérimentale. 2-5 septembre 1998, Lille},
note = {COM},
keywords = {ER4},
pubstate = {published},
tppubtype = {conference}
}
|
1998Conférence ER4 Auteurs : Vandenbulcke, Franck; Nouel, Dominique; Vincent, Jean-Pierre; Beaudet, Alain Differential intracellular trafficking of neurotensin and of its receptor in COS-7 transfected cells 28th annual meeting of the Society for Neuroscience. 7-12 November 1998, Los Angeles (USA), 1998, (ACTI). @conference{Vandenbulcke1998c,
title = {Differential intracellular trafficking of neurotensin and of its receptor in COS-7 transfected cells},
author = {Franck Vandenbulcke and Dominique Nouel and Jean-Pierre Vincent and Alain Beaudet},
year = {1998},
date = {1998-01-01},
booktitle = {28th annual meeting of the Society for Neuroscience. 7-12 November 1998, Los Angeles (USA)},
note = {ACTI},
keywords = {ER4},
pubstate = {published},
tppubtype = {conference}
}
|
1997Conférence ER4 Auteurs : Deloffre, Laurence; Sautière, Pierre-Eric; Vandenbulcke, Franck; Andriès, Jean-Claude Caractérisation biochimique d’une molécule apparentée aux granulines mammaliennes chez un Annélide Polychète, Nereis diversicolor Première Journée Scientifique du réseau LARC-Neurosciences. 3 octobre 1997, Rouen, 1997, (ACTN). @conference{Deloffre1997,
title = {Caractérisation biochimique d’une molécule apparentée aux granulines mammaliennes chez un Annélide Polychète, Nereis diversicolor},
author = {Laurence Deloffre and Pierre-Eric Sautière and Franck Vandenbulcke and Jean-Claude Andriès},
year = {1997},
date = {1997-01-01},
booktitle = {Première Journée Scientifique du réseau LARC-Neurosciences. 3 octobre 1997, Rouen},
note = {ACTN},
keywords = {ER4},
pubstate = {published},
tppubtype = {conference}
}
|
