Publications

@conference{Pelfrene2019,

title = {Simplified method for oral bioaccessibility of metal(loid)s in soils},

author = {Aurélie Pelfrêne},

year  = {2019},

date = {2019-01-01},

booktitle = {ISO/TC 190/SC 7/WG 4 meeting, 15 October 2019, Aveiro (Portugal)},

pages = {18pp},

abstract = {Objectives: Due to the complexity of in vitro methods , a simplification 

	of them might be a great interest for managers of Polluted Sites 

	and Soils Propose a first tier method as a decision making tool Evaluate 

	the potential suitability of chemical single extraction methods to 

	mimic the bioaccessible fraction of As, Cd and Pb in a wide range 

	of soils},

note = {ACTI},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Pelfrene2019a,

title = {Evaluation of four first tier methods to measure metal(loid) bioaccessibility in polluted soils},

author = {Aurélie Pelfrêne and Barbara Le-Bot and Christophe Waterlot and Philippe Glorennec and Karin Sahmer and Francis Douay},

year  = {2019},

date = {2019-01-01},

booktitle = {SETAC Europe, 29th Annual meeting, 26-30 May 2019, Helsinki (Finland)},

note = {ACTI},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Pelfrene2019b,

title = {Méthode simplifiée pour la bioaccessibilité orale des métaux dans les sols},

author = {Aurélie Pelfrêne and Hélène Roussel},

year  = {2019},

date = {2019-01-01},

booktitle = {Journée technique d’information et de retour d’expérience de la gestion des sites et sols pollués 7 novembre 2019, Paris},

abstract = {Objectifs: *En raison de la complexité des méthodes in vitro , leur 

	simplification pourrait présenter un fort intérêt pour les gestionnaires 

	SSP *Proposer une méthode en 1ère approche comme un outil d’aide 

	à la décision *Evaluer la pertinence de méthodes d’extractions chimiques 

	simples pour mimer la fraction bioaccessible fraction de As, Cd et 

	Pb pour une large gamme de sols},

note = {COM},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Pelfrene2019c,

title = {Exposition des jardiniers urbains dans un contexte industrialisé – Aspects méthodologiques},

author = {Aurélie Pelfrêne and Karin Sahmer and Olivier Grard and Christophe Heyman and Francis Douay},

year  = {2019},

date = {2019-01-01},

journal = {Techniques de l’ingénieur},

volume = {10 novembre 2019},

number = {GE1016 V1},

pages = {17pp},

abstract = {INTRODUCTION Jusqu’à récemment le jardinage avait souvent pour vocation 

	principale de subvenir aux besoins alimentaires. De nos jours, les 

	motivations des jardiniers sont beaucoup plus variées. Outre la production 

	de fruits et légumes de qualité, le jardin joue aussi un rôle social 

	et de support de biodiversité fortement dépendant des pratiques associées 

	au jardinage (e.g. utilisation ou non de pesticides, intensité du 

	travail du sol, maintien d’habitats annexes favorables). Si le jardinage 

	est effectué le plus souvent en milieu rural, il se pratique de plus 

	en plus en milieux périurbain et urbain et peut être qualifié « d’agriculture 

	urbaine ». Se pose alors la question de la qualité des sols et des 

	productions cultivées au regard de pollutions potentielles résultant 

	des activités humaines actuelles et/ou passées (chauffage urbain, 

	circulation automobile, activités industrielles, apports de terre/remblais…). 

	D’une façon générale, les connaissances sur les jardins potagers 

	restent insuffisantes au regard de leur nombre élevé, de la diversité 

	de leurs contextes environnementaux, des modes de gouvernance, des 

	pratiques culturales et de consommation, de la complexité des réglementations 

	relatives à ces espaces, privatifs ou pas, et à ces productions. 

 

	L’objectif de cet article est de présenter un retour d’expérience 

	sur une démarche menée, en partenariat avec les services sanitaires, 

	dans une portion de l’ancien bassin minier du Nord-Pas-de-Calais, 

	région densément peuplée et avec un lourd passé industriel. La démarche, 

	transférable à d’autres contextes environnementaux, visait à mieux 

	connaître les jardins majoritairement privatifs. Elle s’étend depuis 

	l’étude historique du site jusqu’à la restitution auprès des jardiniers 

	des données acquises. Ces connaissances sont destinées à alimenter 

	les réflexions sur la gestion des potagers urbains en apportant des 

	arguments scientifiques et techniques sur les quantités produites 

	et consommées, les pratiques culturales des jardiniers, l’aptitude 

	des fruits et légumes autoproduits à accumuler des polluants métalliques. 

	Elles sont aussi le support de réflexions destinées à réduire l’exposition 

	aux polluants métalliques des jardiniers et de leur famille en lien 

	avec le jardinage.},

note = {OV},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Pelfrene2019d,

title = {From environmental data acquisition to assessment of gardeners' exposure: feedback in an urban context highly contaminated with metals},

author = {Aurélie Pelfrêne and Karin Sahmer and Christophe Waterlot and Francis Douay},

year  = {2019},

date = {2019-01-01},

journal = {Environmental Science and Pollution Research},

volume = {26},

number = {20},

pages = {20107-20120},

abstract = {Although growing vegetables in urban gardens has several benefits, 

	some questions in relation with the safety of foods remain when the 

	self-production is carried out on highly contaminated garden soils. 

	To better assess the local population's exposure to Cd and Pb induced 

	by the past activities of a lead smelter, a participatory program 

	was initiated in 115 private kitchen gardens located in northern 

	France to assist gardeners in understanding their soil environment. 

	The challenge included contributing to the database of urban garden 

	soils with the collection of a large number of samples: 1525 crops 

	grouped into 12 types (leaf, fruiting, root, stem and bulbous vegetables, 

	tubers, cabbages, leguminous plants, celeriac, fresh herbs, fruits, 

	and berries), 708 topsoils, and 52 samples of self-produced compost. 

	The main results were as follows: (i) topsoils were strongly contaminated 

	by Cd and Pb compared to regional reference values; (ii) great variability 

	in physicochemical parameters and metal concentrations in topsoils; 

	(iii) the highest concentrations of Cd and Pb for celeriac and fresh 

	herbs and the lowest for fruits and fruiting vegetables; (iv) a high 

	percentage of vegetables that did not comply with the European foodstuff 

	legislation; and (v) most self-produced compost samples were strongly 

	contaminated. This study aimed to raise awareness and generate functional 

	recommendations to reduce human exposure and to provide useful data 

	that could be considered in other environmental contexts.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Remy2019,

title = {Retrouver la qualité des sols : le défi de la reconversion des friches urbaines polluées},

author = {Elisabeth Rémy and Marine Canavese and Nathalie Berthier and Francis Douay and Yves Petit-Berghem},

year  = {2019},

date = {2019-01-01},

booktitle = {4èmes rencontres nationales de la recherche sur les sites et sols pollués, Montrouge, 26 et 27 novembre 2019},

pages = {13pp},

abstract = {Contexte scientifique - Questionnement: Des recherches entre sciences 

	biotechniques, géographie, socio-histoire, Réinterroger la qualité 

	des sols dans le cadre des grandes opérations d’aménagement Etude 

	de cas : Docks de Saint-Ouen Le sol urbain / périurbain : un sol 

	méconnu Le sol périurbain : quels héritages ? Enseignements},

note = {ACTN},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Ridoskova2019,

title = {Bioavailability of mercury in contaminated soils assessed by the diffusive gradient in thin film technique in relation to uptake by Miscanthus x giganteus},

author = {Andrea Ridoskova and Aurélie Pelfrêne and Francis Douay and Pavlina Pelcova and Vendula Smolikova and Vojtech Adam},

year  = {2019},

date = {2019-01-01},

journal = {Environmental Toxicology and Chemistry},

volume = {38},

number = {2},

pages = {321-328},

abstract = {We assessed the relationship between the diffusive gradient in thin 

	film (DGT) technique using the new ion-exchange resin Ambersep GT74 

	and the uptake of mercury (Hg) by a model plant cultivated on metal-contaminated 

	agricultural soils under greenhouse conditions. Based on the total 

	Hg content, 0.37 to 1.17% of the Hg passed to the soil porewater 

	from the solid phase, and 2.18 to 9.18% of the Hg is DGT-available. 

	These results were confirmed by calculating the R value (the ratio 

	of the concentrations of bioavailable Hg measured by DGT and soil 

	solution), which illustrated the strong bonding of Hg to the solid 

	phase of soil and its extremely low mobility. Only inorganic Hg(2+) 

	species were found in the metal-contaminated agricultural soils, 

	as determined by a high-performance liquid chromatography-cold vapor 

	atomic fluorescence spectrometry speciation analysis. The Hg was 

	distributed in Miscanthus x giganteus organs in the following order 

	for all sampling sites: roots (55-82%) >> leaves (8-27%) > stems 

	(7-16%) > rhizomes (4-7%). Environ Toxicol Chem 2019;38:321-328. 

	(c) 2018 SETAC.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Sahmer2019,

title = {Proposition d’un test de permutation dans le cadre d’une régression non linéaire pour l’évaluation de néo-fongicides},

author = {Karin Sahmer and Caroline Deweer and Jérôme Muchembled},

year  = {2019},

date = {2019-01-01},

booktitle = {Séminaire de statistiques et économétrie du LEM, 19 juin 2019, Villeneuve d’Ascq, Campus du Pont-de-Bois},

abstract = {Les plantes cultivées sont régulièrement impactées par des champignons 

	phytopathogènes qui peuvent causer des pertes quantitatives et qualitatives. 

	Si l’utilisation de fongicides chimiques reste la réponse principale, 

	de nouveaux fongicides bio-sourcés apparaissent sur le marché du 

	biocontrôle ou sont en cours de développement grâce à une profonde 

	attente sociétale (respect de l’environnement, développement de l’agriculture 

	biologique, ...) et politique (plan Ecophyto2025, rapport agriculture 

	innovations 2025, ...). L’évaluation de ces bio-fongicides nécessite 

	d’optimiser les méthodes d’évaluations biologiques et statistiques. 

	Ainsi, pour estimer et comparer au laboratoire l’efficacité de bio-fongicides, 

	des essais en microplaques ou en boîtes de Pétri sont réalisés in 

	vitro sur des champignons pathogènes de plantes. L’efficacité des 

	bio-fongicides est évaluée par le calcul d’un indicateur de type 

	CI 50 (la concentration d’inhibition à 50%) grâce à une régression 

	non linéaire dans laquelle la CI 50 est un des paramètres estimés. 

	Pour comparer les CI 50 , deux modèles sont ajustés, l’un avec des 

	CI 50 dépendant du bio-fongicide, l’autre avec la même CI 50 pour 

	tous les bio-fongicides. Un test F est utilisé pour comparer ces 

	modèles. Les conditions d’application de ce test étant questionnables, 

	une procédure de permutation adaptée au modèle est proposée pour 

	le calcul de la p-value ainsi qu’une procédure bootstrap pour le 

	calcul d’intervalles de confiance.},

note = {COM},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Waterlot2019,

title = {Benefits of Ryegrass on Multicontaminated Soils Part 1: Effects of Fertilizers on Bioavailability and Accumulation of Metals},

author = {Christophe Waterlot and Marie Hechelski},

year  = {2019},

date = {2019-01-01},

journal = {Sustainability},

volume = {11},

number = {18},

pages = {5093},

abstract = {Effects of three phosphorus fertilizers on the shoot biomass and on 

	the accumulation of alkali, alkaline earth, and transition metals 

	in the shoots and roots of ryegrass were studied with two contaminated 

	garden soils. Phosphates were added in sustainable quantities in 

	order to reduce the environmental availability of carcinogenic metals 

	(e.g., Cd and Pb) and to enhance the bioavailability of alkali and 

	alkaline earth metals as well as micronutrients needed by plants. 

	Addition of Ca(H2PO4)2 was the most convenient way to (i) limit the 

	concentration of Cd and Pb, (ii) keep constant the transfer of macro- 

	and micronutrient from the soil to the ryegrass shoots, (iii) decrease 

	the availability of metals, and (iv) increase the ratio values between 

	potential Lewis acids and Cd or Pb in order to produce biosourced 

	catalysis. For instance, the real phytoavailability was reduced by 

	27%–57% and 64.2%–94.8% for Cd and Pb, respectively. Interestingly, 

	the real phytoavailability of Zn was the highest in the least contaminated 

	soils. Even if soils were highly contaminated, no visual toxicity 

	symptoms were recorded in the growing ryegrasses. This indicates 

	that ryegrass is suitable for the revegetation of contaminated gardens. 

	To promote the sustainable ryegrass production on contaminated soils 

	for production of new organic fragrance and drugs in green processes 

	according to REACH (Registration, Evaluation, Authorisation, and 

	Restriction of Chemicals) regulation, two processes should be recommended: 

	assisted phytostabilization of the elements, and then assisted phytoextraction 

	by using chelators. View Full-Text},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Andrianarisoa2019,

title = {Agroforestry in the Hauts-de-France -A Research and Demonstration Experimental Site in Ramecourt},

author = {Sitraka Andrianarisoa and François Delbende and Christelle Pruvot and Caroline Choma and Marie-Astrid Bouchard and Alan Guillou and Antoine Dequidt and Bernhard Zeller and Sandrine Oste and Karine Petit and Bertrand Vandoorne},

year  = {2019},

date = {2019-01-01},

booktitle = {4th World Congress on Agroforestry, 20-22 mai 2019, Montpellier},

pages = {1p},

abstract = {As the current agricultural practices in the Hauts-de-France region 

	result in soil erosion, nitrate leaching and a decline in biodiversity, 

	agroforestry systems (AFs) may be an alternative to conciliate productivity 

	with lower environmental impact (Dupraz and Liagre, 2008). We set 

	up the first AF experimental site to study its agro-economic and 

	environmental performance in local agro-pedoclimatic conditions. 

	The experimental site was established in autumn 2018 on an 18-ha 

	plot in Ramecourt on a deep luvic cambisol with a silt loam texture 

	developed on a flint clay. Due to a high silt content (73% silt) 

	and a low organic matter content (2%), as well as a slope of 8%, 

	the plot is highly affected by channel erosion. Modalities with or 

	without nitrogen-fixing trees in AF treatment are compared with sole-crop 

	(CC) and pure-forest control (FC) plots (Figure 1A) according to 

	a randomised block design with 3 replicates. Tall trees in rows are 

	intercalated with 9 species of shrubs (Figure 1B) and will be intercropped 

	in AFs by sugar beet, potato, wheat, barley and flax. The tree density 

	is 50 and 430 trees ha-1 for AFs and FC respectively, and the average 

	size of the microplots is 0.9 ha. Using this experimental approach, 

	we hypothesised that AFs should limit soil erosion, restore soil 

	fertility and biodiversity, improve natural-resource use efficiency 

	and water quality, reduce inputs and increase farmers’ incomes.},

note = {AFF},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Bidar2019b,

title = {Evaluation in situ des effets d’amendements sur le comportement du blé cultivé sur une parcelle agricole contaminée par les activités passées d’une fonderie de plomb},

author = {Géraldine Bidar and Brice Louvel and Adeline Janus and Jacques Blarel and Guillaume Lemoine and Francis Douay},

year  = {2019},

date = {2019-01-01},

booktitle = {4ème Rencontres nationales de la Recherche sur les sites et sols pollués, Le Beffroi de Montrouge, 26-27 novembre 2019, Paris},

note = {ACTN},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Bidar2019a,

title = {In situ assessment of soil amendment effects on hemp behavior in soils highly contaminated by past smelter activities},

author = {Géraldine Bidar and Brice Louvel and Adeline Janus and Jacques Blarel and Guillaume Lemoine and Francis Douay},

year  = {2019},

date = {2019-01-01},

booktitle = {Intersol’2019, International Conference-Exhibition on Soils, Sediments and Water, 26-28 mars 2019, Lille},

note = {ACTN},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Desmyttere2019,

title = {Antifungal activities of Bacillus subtilis lipopeptides to two Venturia inaequalis strains possessing different tebuconazole sensitivity},

author = {Hélène Desmyttère and Caroline Deweer and Jérôme Muchembled and Karin Sahmer and Justine Jacquin and François Coutte and Philippe Jacques},

year  = {2019},

date = {2019-01-01},

journal = {Frontiers in Microbiology},

volume = {10},

number = {2327},

pages = {1-10},

abstract = {Within the framework of biocontrol development, three natural substances 

	produced by Bacillus subtilis, called lipopeptides, have been studied: 

	fengycin (F), surfactin (S) and mycosubtilin (M). Their antifungal 

	properties were tested in vitro, in liquid medium, on two strains 

	of Venturia inaequalis, ascomycete fungi causing apple scab. These 

	two strains were respectively sensitive and less sensitive to tebuconazole, 

	an active substance of the triazole family. These three molecules 

	were tested on their own, in binary (FS, FM, SM) and ternary mixtures 

	(FSM). The antifungal activities of lipopeptides were estimated by 

	calculating an IC50, compared to tebuconazole chemical substance. 

	In tests involving the sensitive strain, all lipopeptide modalities 

	exhibited antifungal activity. However, modalities involving fengycin 

	and its mixtures exhibited the best antifungal activities; the activity 

	of fengycin alone being very similar to that of tebuconazole. Interestingly, 

	regarding the strain with reduced sensitivity to tebuconazole, surfactin 

	and fengycin alone were not efficient while mycosubtilin and the 

	different mixtures showed interesting antifungal activities. Specifically, 

	the antifungal activity of FS and FSM mixture were equivalent to 

	that of tebuconazole. For both fungal strains, microscopic observations 

	revealed important morphological modifications in the presence of 

	fengycin and in a less important proportion in the presence of surfactin 

	but not in the presence of mycosubtilin. Overall, this study highlights 

	the diversity in mode of action of lipopeptides on apple scab strains.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@techreport{Detriche2019,

title = {Carte pédologique à 1/50000 dans la région de Béthune et de Lens; Carte, notice et données saisies sous Donesol, programme CPF, IGCS},

author = {Sébastien Détriché},

year  = {2019},

date = {2019-01-01},

institution = {ISA-LGCgE},

note = {AP},

keywords = {ER4},

pubstate = {published},

tppubtype = {techreport}

}

@techreport{Douay2019,

title = {Jardins potagers : retour sur 30 ans de recherche en connaissance, évaluation et gestion des impacts},

author = {Francis Douay and Franck Marot and Christophe Schwartz},

year  = {2019},

date = {2019-01-01},

abstract = {Si la pratique du jardinage est ancestrale, la curiosité des chercheurs 

	pour la qualité de l’écosystème jardiné, en particulier dans les 

	disciplines des sciences du sol, des sciences végétales, de l’agronomie, 

	de la toxicologie ou de l’écotoxicologie n’est réellement apparue 

	en France que depuis 3 décennies. Sur cette courte période, des connaissances, 

	progressivement disciplinaires puis transdisciplinaires, ont été 

	acquises qui permettent aujourd’hui d’avoir une appréciation globale 

	de la qualité des sols en termes de fertilité et de contamination. 

 

	L’évaluation de l’aptitude des jardins à assurer des fonctions écosystémiques 

	essentielles et à rendre des bouquets de services aux jardiniers 

	et plus généralement aux citadins est envisageable. 

 

	Connaître l’écosystème « jardin potager », avec parfois la mise en 

	lumière de ses excès de fertilité ou de contamination, nécessite 

	alors de mieux le gérer. A partir de l’observation des jardins existants, 

	des bases de données sont disponibles, des risques de dissémination 

	de contaminants sont évaluables, des recommandations de pratiques 

	sont possibles et des modes de gestion sont préconisables. 

 

	Pour les futures implantations de jardins, il reste à amplifier la 

	prise en compte de la qualité des sols dans les stratégies d’aménagement. 

	L’enjeu est de maintenir une nature jardinée en ville qui contribue 

	à atténuer les effets négatifs des changements globaux, à rendre 

	des services et à procurer du bien-être aux générations futures de 

	jardiniers et de citadins},

note = {OS},

keywords = {ER4},

pubstate = {published},

tppubtype = {techreport}

}

@conference{Douay2019a,

title = {Jardins potagers : retour sur 30 ans de recherche en connaissance, évaluation et gestion des impacts},

author = {Francis Douay and Christophe Schwartz},

year  = {2019},

date = {2019-01-01},

booktitle = {4ème Rencontres nationales de la Recherche sur les sites et sols pollués, Le Beffroi de Montrouge, 26-27 novembre 2019, Paris},

abstract = {Si la pratique du jardinage est ancestrale, la curiosité des chercheurs 

	pour la qualité de l’écosystème jardiné, en particulier dans les 

	disciplines des sciences du sol, des sciences végétales, de l’agronomie, 

	de la toxicologie ou de l’écotoxicologie n’est réellement apparue 

	en France que depuis 3 décennies. Sur cette courte période, des connaissances, 

	progressivement disciplinaires puis transdisciplinaires, ont été 

	acquises qui permettent aujourd’hui d’avoir une appréciation globale 

	de la qualité des sols en termes de fertilité et de contamination. 

 

	L’évaluation de l’aptitude des jardins à assurer des fonctions écosystémiques 

	essentielles et à rendre des bouquets de services aux jardiniers 

	et plus généralement aux citadins est envisageable. 

 

	Connaître l’écosystème « jardin potager », avec parfois la mise en 

	lumière de ses excès de fertilité ou de contamination, nécessite 

	alors de mieux le gérer. A partir de l’observation des jardins existants, 

	des bases de données sont disponibles, des risques de dissémination 

	de contaminants sont évaluables, des recommandations de pratiques 

	sont possibles et des modes de gestion sont préconisables. 

 

	Pour les futures implantations de jardins, il reste à amplifier la 

	prise en compte de la qualité des sols dans les stratégies d’aménagement. 

	L’enjeu est de maintenir une nature jardinée en ville qui contribue 

	à atténuer les effets négatifs des changements globaux, à rendre 

	des services et à procurer du bien-être aux générations futures de 

	jardiniers et de citadins},

note = {ACTN},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Hechelski2019b,

title = {Effects of calcium phosphates on the (im)mobilization of metals and nutrients, on the biological activity and on the plant health from multi-contaminated urban soils},

author = {Marie Hechelski and Brice Louvel and Pierrick Dufrénoy and Alina Ghinet and Christophe Waterlot},

year  = {2019},

date = {2019-01-01},

journal = {Water, Air, & Soil Pollution},

volume = {230},

number = {8},

pages = {206-223},

abstract = {Two smelters in the North of France emitted potentially toxic metals 

	for more than a century and today, the resulting contamination represents 

	a risk to human health and affects also the biodiversity. To limit 

	health risks and to improve the soil quality, a study using calcium 

	phosphates (monocalcium phosphate, dicalcium phosphate and a mixture 

	of both salts) and Lolium perenne L was conducted. Through this preliminary 

	investigation, we will try to shed some light about (i) the effects 

	of a sustainable amount of calcium phosphates on the agronomic, biological 

	(microbial and fungi communities) and physiological parameters (chlorophyll 

	a and b, antocyanins, carotenoids) as well as the phytoavailability 

	of potentially toxic metals and nutrients in time, and (ii) the potential 

	use of contaminated biomass from ryegrass as a source of new valorisation 

	ways instead of using it as contaminated compost by gardeners. Although 

	slight variations in pH and significant increases of assimilable 

	phosphorus after adding calcium phosphates were registered, the physiology 

	of plants and the biological parameters were statistically unchanged. 

	The germination of the ryegrass seeds was favoured with calcium phosphates 

	regardless the contamination level of the studied soils. No clear 

	effects of calcium phosphates on the microbial and fungi communities 

	were detected. In contrast, results indicated relationships between 

	the physicochemical parameters of soils, their contamination level 

	and the composition of fungal communities. Indeed, for one of the 

	soils studied, calcium could limit the transport of nutrients, causing 

	an increase in fungi to promote again the transfer of nutrients. 

	Surprisingly, the phytoavailability of Pb increased in the most contaminated 

	soil after adding dicalcium phosphate and the mixture of phosphates 

	whereas a slight decrease was highlighted for Cd and Mn. Although 

	minor changes in the phytoavailability of potentially toxic metals 

	were obtained using calcium phosphates, the ability of ryegrass to 

	accumulate Zn and Ca (up to 600 and 20,000 mg kg−1, respectively) 

	make possible to qualify this plant as a bio ‘ore’ resource.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Hechelski2019ab,

title = {A sustainable approach to manage metal-contaminated soils: a preliminary greenhouse study for the possible production of metal-enriched ryegrass biomass for biosourced catalysts},

author = {Marie Hechelski and Brice Louvel and Pierrick Dufrénoy and Alina Ghinet and Christophe Waterlot},

year  = {2019},

date = {2019-01-01},

journal = {Environmental Monitoring and Assessment},

volume = {191},

number = {10},

pages = {626-640},

abstract = {Two kitchen garden soils (A and B) sampled in contaminated areas were 

	amended using phosphates in sustainable quantities in order to reduce 

	the environmental availability of potentially toxic inorganic elements 

	(PTEs) and to favour the availability of alkali, alkali earth and 

	micronutrients. The environmental availability of PTEs was evaluated 

	using a potential plant for revegetation of contaminated soils (ryegrass) 

	and a mixture of low molecular weight organic acids. Despite the 

	highest contamination level of B, the concentration of metals was 

	highest in the ryegrass shoots grown on A for the two harvests. These 

	results correlated well with those obtained using low molecular weight 

	organic acids for Cd, Zn and Cu, whereas this mixture failed to represent 

	the transfer of nutrients due to the presence of biological and physiological 

	mechanisms. The statistical differences between the biomass of ryegrass 

	obtained at the first and the second harvests were attributed to 

	the decrease of available potassium, implicated in the growth and 

	development of plants. Phosphates increased the ratios Zn/Cd, Zn/Pb 

	and Zn/Cu up to 176 ± 48, 38 ± 6 and 80 ± 12, respectively, and made 

	possible the reduction of the concentration of Cd and Pb in the shoots 

	of ryegrass by 22% and 25%, respectively. The concentration of Zn 

	in the shoots of ryegrass from the first and the second harvests 

	grown on soil A were in the range 1050–2000 mg kg−1, making this 

	plant a potential biomass to (i) produce biosourced catalysts for 

	organic chemistry applications in a circular economy concept and 

	(ii) limit human exposure to commercial Lewis acids. A preliminary 

	application was identified.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Leclercq-Dransar2019,

title = {Distribution of metals and cell wall compounds in leaf parts of three tree species suitable for the phytomanagement of heavy metal–contaminated soils},

author = {Julie Leclercq-Dransart and Sylvain Demuynck and Christophe Waterlot and Géraldine Bidar and Karin Sahmer and Céline Pernin and A Deram and Alain Leprêtre and Francis Douay},

year  = {2019},

date = {2019-01-01},

journal = {Water Air and Soil Pollution},

volume = {230},

number = {237},

pages = {1-16},

abstract = {Phytomanagement used on soils contaminated with metals aims to stabilize 

	them in the soil. It generally uses less demanding tree species such 

	as maple, poplar, and black locust. However, it is important to consider 

	the rate of accumulation of metals in the leaves as well as their 

	location (leaf blade/petiole) together with the contents of cell 

	wall compounds (hemicelluloses, cellulose, lignin). These two aspects 

	are likely not only to have repercussions on the decomposition of 

	litter but also to lead to a possible transfer ofmetal contamination 

	into the food chain via soil decomposers/detritivorous. A successful 

	phytoremediation is therefore the result of compromise: no tree species 

	can meet all these criteria. The results obtained show that poplar 

	is not recommended due to a high accumulation of Cd and Zn. The most 

	appropriate species among our 3 studied species, which seem the most 

	suitable in terms of remediation of metal polluted soils, could be 

	the black locust and the maple which are less susceptible to contribute 

	to the transfer of exogenous metals such as Cd to the trophic chain. 

	However, maple strongly accumulates Pb in the leaf blade and it is 

	susceptible to degrade quickly due to its high hemicelluloses and 

	cellulose contents and low lignin content. The black locust accumulates 

	metals mainly in the petiole (little consumed by detritivorous). 

	However, its exotic character and high lignin content may limit the 

	interest of its use.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Leclercq-Dransart2019b,

title = {Isopod physiological and behavioral responses to drier conditions: An experiment with four species in the context of global warming},

author = {Julie Leclercq-Dransart and Céline Pernin and Sylvain Demuynck and Fabien Grumiaux and Sébastien Lemière and Alain Leprêtre},

year  = {2019},

date = {2019-01-01},

journal = {European Journal of Soil Biology},

volume = {90},

pages = {22-30},

abstract = {In the context of global warming, an increase in soil drought is suspected 

	by the IPCC predictions and litter breakdown activity could be affected. 

	An experiment was conducted using microcosms (controlled conditions) 

	and woodlice which are recognized as shredders of litter and bioindicators 

	of global warming. The impact of relative air humidity (90 and 50% 

	RH) on litter breakdown by woodlice was studied through the monitoring 

	of one behavioral (distribution of individuals on microcosms), one 

	morphological (Absolute Growth Rate) and four physiological traits 

	(Survival rate, Relative Consumption Rate, Relative Growth Rate, 

	Feeding rate). Four species of isopods known to have different microclimatic 

	sensitivities (Armadillidium vulgare, Porcellio scaber, Oniscus asellus, 

	Philoscia muscorum) were used simultaneously. Sensitivities of males 

	and females have also been tested. Main results showed that the seven 

	studied parameters under the two relative humidity conditions were 

	not affected in the same way according to the species nor genders. 

	A. vulgare is the most affected species followed by O. asellus. The 

	least affected species are P. scaber and P. muscorum. Their morphological 

	and physiological differences allow to the most evolved species to 

	be more resistant to drier conditions but in return they are no longer 

	active, which can affect soil functionality. With global warming, 

	it could be envisaged that woodlice spend less time foraging and 

	more time seeking refuge to reduce the risk of mortality from desiccation 

	stress. Modifying the feeding behavior of terrestrial isopods could 

	slow down litter degradation with consequences on the process of 

	organic matter recycling.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Louvel2019,

title = {Développement d’une économie biosourcée franco-belge liée au phytomanagement de sites marginaux},

author = {Brice Louvel and Valérie Bert and Aricia Evlard and Erik Meers},

year  = {2019},

date = {2019-01-01},

booktitle = {4ème Rencontres nationales de la Recherche sur les sites et sols pollués, Le Beffroi de Montrouge, 26-27 novembre 2019, Paris},

abstract = {Le projet Interreg France-Wallonie-Flandres New-C-Land (2018-2021) 

	contribue au développement de l'économie biosourcée et encourage 

	la production durable de biomasse végétale utilisée en énergie et 

	matières sur sites marginaux (surfaces délaissées non cultivées). 

	La COP 21 et le programme Climat et énergie 2030 invitent les pays 

	à réduire leur dépendance aux sources fossiles, à réduire les émissions 

	de gaz à effet de serre et à mettre en oeuvre des activités économiques 

	durables. Dans ce cadre, le projet New-C-Land a reçu le soutien du 

	Fonds européens de développement régional (FEDER) pour identifier 

	des sites abandonnés, sous-utilisés, souvent négligés et parfois 

	pollués. Le projet vise à stimuler la bio-économie sur les 3 territoires 

	en développant sur des surfaces marginales des cultures non alimentaires 

	de biomasses pour être utilisées localement. New-C-Land a pour objectifs 

	(i) d’identifier les terres marginales en Flandre, Wallonie et dans 

	les Hauts de France, (ii) mettre en contact les propriétaires fonciers 

	et les utilisateurs de la biomasse afin d’encourager le développement 

	de chaînes de valeur industrielles, (iii) apporter un outil cartographique 

	et d’aide à la décision et (iv) tester les filières de valorisation 

	identifiées grâce à l’outil d’aide à la décision in situ sur pilotes 

	et à l’échelle industrielle.},

note = {ACTN},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Louvel2019a,

title = {Évaluation de la phytodisponibilité des éléments métalliques présents dans un digestat solide épandu sur une terre contaminée ou non},

author = {Brice Louvel and Mathieu Bossuwe and Francis Douay},

year  = {2019},

date = {2019-01-01},

booktitle = {4ème Rencontres nationales de la Recherche sur les sites et sols pollués, Le Beffroi de Montrouge, 26-27 novembre 2019, Paris},

abstract = {L’épandage de digestats de méthanisation, issus d’intrants agricoles 

	provenant d’une zone contaminée en métaux, interroge sur le devenir 

	des polluants et le comportement des cultures. Pour apporter des 

	éléments de réponse, des digestats de méthanisation ont été élaborés 

	à partir de productions issues ou non de la zone affectée massivement 

	par les émissions passées de Metaleurop Nord (Noyelles-Godault). 

	Une culture de ray-grass a été réalisée en serre sur deux terres 

	prélevées dans l’horizon labouré de deux parcelles situées ou non 

	sous l’influence de l’ancienne fonderie de plomb et de zinc. Ce protocole 

	expérimental a été complété avec une modalité comportant une fertilisation 

	minérale NPK et un témoin (eau). Sur la terre non contaminée, l’ajout 

	des digestats solides contaminés ou non n’a pas eu d’effet sur le 

	rendement des feuilles de ray-grass alors que sur la terre contaminée 

	a été constaté un accroissement notable de la biomasse. La contamination 

	de la terre contaminée a été soulignée par la modalité témoin (eau) 

	; les concentrations en Cd, Pb et Zn dans les parties aériennes du 

	ray-grass cultivé sur la terre contaminée sont respectivement près 

	de 5, 2 et 5 fois plus élevées que celles se rapportant au ray-grass 

	récolté sur la terre non contaminée. Les concentrations en Cd et 

	Zn du ray-grass cultivé sur la terre non contaminée amendée n’ont 

	pas montré de différence avec celles du témoin. L’apport de digestat 

	issu des productions de la zone Metaleurop a augmenté les concentrations 

	en Cd et Zn dans les feuilles de ray-grass cultivé sur la terre contaminée 

	par rapport au témoin. Toutefois, cet accroissement n’a pas été significativement 

	différent de celui constaté avec l’apport de digestat issu de végétaux 

	non contaminés ou de fertilisants minéraux. Ceci laisse donc penser 

	que l’accroissement des concentrations en métaux dans le ray-grass 

	est davantage lié à la contamination des sols qu’aux contaminants 

	apportés avec les digestats.},

note = {ACTN},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Louvel2019b,

title = {Expérimentation de phytomanagement sur les sols de l’ancien site Rhodia de Saint-André-Lez-Lille},

author = {Brice Louvel and Tanguy Latron and Damien Delsarte and Francis Douay},

year  = {2019},

date = {2019-01-01},

booktitle = {4ème Rencontres nationales de la Recherche sur les sites et sols pollués, Le Beffroi de Montrouge, 26-27 novembre 2019, Paris},

abstract = {La reconquête des friches industrielles en coeur des métropoles est 

	un enjeu majeur dans le cadre du renouvellement urbain. La période 

	de transition vers un nouvel usage des sites peut s’échelonner sur 

	plusieurs années. Pendant ce laps de temps, il est important de répondre 

	aux attentes environnementales (confinement des contaminants sur 

	le site…) et sociétale (amélioration du cadre de vie des riverains…). 

	Les couvertures végétales peuvent apporter une réponse à ces contraintes. 

	La diversité des espèces végétales couramment utilisées est cependant 

	restreinte. Une expérimentation de phytomanagement on-site a été 

	mise en place de 2015 à 2018 sur un ancien site industriel lié à 

	la chimie minérale et ceci, en vue d’évaluer l’aptitude de différents 

	couverts végétaux à se développer sur des matériaux multi-contaminés 

	(fortes anomalies en As, Hg et Pb). Une plateforme expérimentale 

	comportant 14 placettes a été créée pour être dédiée à l’étude de 

	12 espèces végétales sélectionnées au regard de leur comportement 

	vis-à-vis des polluants métalliques et de leur aptitude à les accumuler 

	dans leurs organes aériens (Medicago lupulina, Brassica juncea, Agrostis 

	capillaris, Salix alba, Lolium perenne, Anthyllis vulneraria, Festuca 

	rubra, Festuca ovina, Achillea millefolium, Deschampsia cespitosa, 

	Valeriana officinalis, Miscanthus x giganteus). Lolium perenne a 

	été semé seul ou en association avec Salix alba. Après trois années 

	de suivi, seules quatre espèces initialement installées étaient encore 

	présentes se sont adaptées aux conditions expérimentales. Les teneurs 

	en contaminants inorganiques (As, Cd, Cr, Zn) dans les parties aériennes 

	ont été supérieures dans le Miscanthus x giganteus par rapport à 

	celles mesurées dans Agrostis capillaris, Festuca ovina et Festuca 

	rubra.},

note = {ACTN},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Louvel2019c,

title = {Evaluation in situ de modes de gestion appliqués sur une friche multicontaminée de la carbochimie},

author = {Brice Louvel and Guillaume Lemoine and Sébastien Détriché and Géraldine Bidar and Adeline Janus and Francis Douay},

year  = {2019},

date = {2019-01-01},

booktitle = {4ème Rencontres nationales de la Recherche sur les sites et sols pollués, Le Beffroi de Montrouge, 26-27 novembre 2019, Paris},

abstract = {La reconquête des friches industrielles présente des enjeux environnementaux 

	et sanitaires d’envergure. Dans cet objectif, les phytotechnologies 

	sont présentées dans la littérature comme un mode de gestion innovant, 

	transitoire ou pérenne, et limitant la dispersion des polluants en 

	protégeant les sols de l’érosion. Elles peuvent réduire la disponibilité 

	des polluants. Néanmoins, ces techniques manquent encore de recul 

	opérationnel [1]. Le projet MisChar, soutenu par l’ADEME, vise à 

	apporter un regard scientifique, technique et économique sur différents 

	modes de gestion appliqués sur des sols multicontaminés par des activités 

	industrielles passées. Une expérimentation a été mise en place avec 

	pour objectif d’étudier les effets de modes de gestion basés sur 

	l’utilisation d’un amendement des sols, l’installation de communautés 

	végétales et des pratiques usuelles d’entretien des espaces verts. 

	Elle concerne une parcelle située dans l’ancien Bassin minier du 

	Nord – Pas de Calais sur laquelle ont été déposés des matériaux multicontaminés 

	issus de la carbochimie (usines de Mazingarbe). Au printemps 2018, 

	20 placettes d’une surface de 20 m² ont été définies. Le sol de 10 

	de ces placettes a été amendé au moyen d’un mélange de biochar de 

	miscanthus et de compost de déchets verts. Les 10 autres placettes 

	ont été utilisées comme témoin. A chacune de ces placettes correspond 

	un type d’ensemencement particulier et un mode de gestion distinct. 

	Ainsi, diverses essences végétales ont été semées. Il s’agit (1) 

	d’un mélange d’espèces classiquement mis en oeuvre sur les espaces 

	verts (ray-grass anglais, fétuque rouge gazonnante, fétuque rouge 

	traçante et pâturin des prés), (2) d’un mélange d’espèces sélectionnées 

	pour leurs comportements présumés vis-à-vis des polluants (stabilisation 

	des polluants métalliques, rhizo/phytodégradation de certains polluants 

	organiques (ray-grass anglais, agrostide stolonifère, fétuque rouge, 

	trèfle blanc), et des mêmes espèces en implantation monospécifique 

	avec (3) un ray-grass, (4) une agrostis stolonifère, (5) un trèfle 

	blanc, et (6) une fétuque rouge. Une septième modalité correspond 

	à l’installation d’une végétation spontanée. Aux espèces implantées 

	en mélange et à la végétation spontanée, correspondent deux modes 

	de gestion (tonte ou pas du couvert végétal). Durant les trois années 

	d’expérimentation seront étudiés le comportement des polluants des 

	sols, les effets des modes de gestion sur les communautés végétales 

	et d’une façon globale, sur la fonctionnalité des sols.},

note = {ACTN},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Louzon2019c,

title = {Couplage de la bioaccessibilité orale pour l’homme et de la biodisponibilité pour l’escargot},

author = {Maxime Louzon and Benjamin Pauget and Aurélie Pelfrêne and Frédéric Gimbert and Nadia Crini and Dominique Rieffel and Caroline Amiot and Francis Douay and Annette De-Vaufleury},

year  = {2019},

date = {2019-01-01},

booktitle = {4ème Rencontres nationales de la Recherche sur les sites et sols pollués, Le Beffroi de Montrouge, 26-27 novembre 2019, Paris},

abstract = {Dans le contexte de l’évaluation des risques sanitaires (ERS) et environnementaux 

	(ERE), un des objectifs du programme COMBINE, soutenu par l’ADEME 

	est de confronter les outils utilisés dans ces évaluations. Sur vingt-neuf 

	échantillons de terres contaminées par l’As, le Cd et le Pb, ont 

	été réalisées d’une part, un test in vitro de bioaccessibilité pour 

	l’homme (UBM) estimant la fraction des contaminants solubilisée au 

	niveau du tractus gastro-intestinal après ingestion de particules 

	de terre, et d’autre part, un test de bioaccumulation ex situ en 

	utilisant l’escargot. Les résultats montrent une influence des paramètres 

	physico-chimiques des terres sur la bioaccessibilité et la bioaccumulation 

	des contaminants par l’escargot. Les régressions multiples révèlent 

	de bonnes corrélations entre la bioaccumulation chez l’escargot et 

	la bioaccessibilité (r²adj entre 0,77 et 0,95) selon la nature et 

	la concentration du contaminant, des paramètres physico-chimiques 

	des terres, et de la phase gastrique ou gastro-intestinale du test 

	UBM. Pour les trois éléments métalliques étudiés, une forte relation 

	existe entre les méthodologies d’évaluation de la biodisponibilité 

	pour l’ERE et l’ERS. Ceci n’avait jamais encore été montré pour des 

	organismes présentant une physiologie et des modes d’exposition variés},

note = {ACTN},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Marchand2019,

title = {Adverse effects of fly ashes used as immobilizing agents for highly metal-contaminated soils on Xenopus laevis oocytes survival and maturation-a study performed in the north of France with field soil extracts},

author = {Guillaume Marchand and Sylvain Demuynck and Sylvain Slaby and Arlette Lescuyer and Sébastien Lemière and Matthieu Marin},

year  = {2019},

date = {2019-01-01},

journal = {Environmental Science and Pollution Research},

volume = {27},

pages = {3706–3714},

abstract = {Amphibians are now recognized as the most endangered group. One of 

	this decline causes is the degradation of their habitat through direct 

	contamination of water, soil leaching, or runoff from surrounding 

	contaminated soils and environments. In the North of France, the 

	extensive industrial activities resulted in massive soil contamination 

	by metal compounds. Mineral amendments were added to soils to decrease 

	trace metal mobility. Because of the large areas to be treated, the 

	use of inexpensive industrial by-products was favored. Two types 

	of fly ashes were both tested in an experimental site with the plantation 

	of trees in 2000. Aim of the present work was to investigate the 

	effects of extracts from metal-contaminated soils treated or not 

	for 10 years with fly ashes on Xenopus laevis oocyte using cell biology 

	approaches. Indeed, our previous studies have shown that the Xenopus 

	oocyte is a relevant model to study the metal ion toxicity. Survival 

	and maturation of oocyte exposed to the soil extracts were evaluated 

	by phenotypic approaches and electrophysiological recordings. An 

	extract derived from a metal-contaminated soil treated for 10 years 

	with sulfo-calcic ashes induced the largest effects. Membrane integrity 

	appeared affected and ion fluxes in exposed oocytes were changed. 

	Thus, it appeared that extracted elements from certain mineral amendments 

	used to prevent the mobility of metals in the case of highly metal-contaminated 

	soils could have a negative impact on X. laevis oocytes.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Ors2019,

title = {A plant nutrient-and microbial protein-based resistance inducer elicits wheat cultivar-dependent resistance against Zymoseptoria tritici},

author = {Marie-Eva Ors and Béatrice Randoux and Ali Siah and Gilles Couleaud and Claude Maumené and Karin Sahmer and Philippe-Lucien Reignault and Patrice Halama and Sameh Selim},

year  = {2019},

date = {2019-01-01},

journal = {Phytopathology},

volume = {109},

number = {12},

pages = {2033-2045},

abstract = {The induction of plant defense mechanisms by resistance inducers is 

	an attractive and innovative alternative to reduce the use of fungicides 

	on wheat against Zymoseptoria tritici, the responsible agent of Septoria 

	tritici blotch (STB). Under controlled conditions, we investigated 

	the resistance induction in three wheat cultivars with different 

	susceptible levels to STB as a response to a treatment with a sulfur, 

	manganese sulfate and protein-based resistance inducer (NECTAR Céréales®). 

	While no direct anti-germination effect of the product was observed 

	in planta, more than 50 % reduction of both symptoms and sporulation 

	were recorded on the three tested cultivars. However, an impact of 

	the wheat genotype on resistance induction was highlighted, which 

	affects host penetration, cell colonization and the production of 

	cell-wall degrading enzymes by the fungus. Moreover, in the most 

	susceptible cultivar Alixan, the product upregulated POX2, PAL, PR1 

	and GLUC gene expression in both non-inoculated and inoculated plants 

	and CHIT2 in non-inoculated plants only. In contrast, defense responses 

	induced in Altigo, the most resistant cultivar, seem to be more specifically 

	mediated by the phenylpropanoid pathway in non-inoculated as well 

	as inoculated plants, since PAL and CHS were most specifically upregulated 

	in this cultivar. In Premio, the moderate resistant cultivar, NECTAR 

	Céréales® elicits mainly the octadecanoid pathway, via LOX and AOS 

	induction in non-inoculated plants. We concluded that this complex 

	resistance-inducing product protects wheat against Z. tritici by 

	stimulating the cultivar-dependent plant defense mechanisms.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@book{Ouni2019,

title = {Assessment of heavy metals along a contamination gradient in soils collected from industrial areas in northern Tunisia},

author = {Ahmed Ouni and Chedliya Ghemari and Amina Ben-Said and Christelle Pruvot and Francis Douay and Karima Nasri-Ammar},

year  = {2019},

date = {2019-01-01},

booktitle = {Recent Advances in Geo-Environmental Engineering, Geomechanics and Geotechnics, and Geohazards},

pages = {45-48},

abstract = {Many studies were interested in the assessment and the monitoring 

	of soil contamination with heavy metals. This study aimed to evaluate 

	the effects of emissions from the Tunisian Steel Company El Fouladh 

	in Bizerte (Tunisia). In this context, we measured Zinc (Zn), Copper 

	(Cu), Lead (Pb) and Cadmium (Cd) soil’s concentrations from 8 sites 

	along a contamination gradient. Besides, physicochemical parameters, 

	pH, organic carbon, CEC, and CaCO3 were measured. Heavy metals were 

	determined using an atomic absorption spectrometry. The results revealed 

	a high concentration of heavy metals in the sampled soils. The contamination 

	factor (CF) was determined to assess the soil contamination of the 

	sampling sites, and revealed that the level of contamination varied 

	along a contamination gradient, which may be due to the mobility 

	of metals.},

note = {OS},

keywords = {ER4},

pubstate = {published},

tppubtype = {book}

}

@article{Ouni2019a,

title = {Monitoring of heavy metal contamination in soils and terrestrial isopods sampled from the industrialized areas of Sfax (southeastern Tunisia)},

author = {Ahmed Ouni and Chedliya Ghemari and Amina Ben-Said and Christelle Pruvot and Francis Douay and Karima Nasri-Ammar},

year  = {2019},

date = {2019-01-01},

journal = {Environmental Earth Sciences},

volume = {78},

number = {440},

pages = {1-13},

abstract = {To evaluate the influence of soil contamination on the distribution 

	of terrestrial isopods, species richness, relative abundance, and 

	diversity, various indices were studied in 20 sampling sites from 

	five industrial areas located in Sfax (SE Tunisia). The following 

	hypotheses were tested: (1) is isopod diversity affected by soil 

	properties? (2) What are the environmental factors that influence 

	the isopod distribution? A total of 445 individuals were sampled 

	and eight species of terrestrial isopods were identified. Porcellionides 

	pruinosus was the most abundant species (38%); however, the highest 

	specific richness per area was equal to four species. To evaluate 

	the impact of metal contamination on isopod distribution, cadmium 

	(Cd), lead (Pb), zinc (Zn), copper (Cu), and iron (Fe) concentrations 

	were measured in soils and terrestrial isopods. Moreover, multivariate 

	analyses revealed a negative correlation between heavy metal concentrations 

	and ecological parameters suggesting an impact of the contamination 

	degree on biodiversity. In addition, to characterize the heavy metal 

	accumulation in isopods, the bioaccumulation factor (BAF) was determined 

	and revealed that Cu and Zn were the most accumulated elements. Results 

	showed that for all species, the order of the BAF values is as follows: 

	Cu ≫ Zn ≫ Cd > Pb > Fe. On the contrary, all species seemed to be 

	deconcentrators of Fe and Cd, with some exceptions.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Pauget2019,

title = {Intégration de la biodisponibilité dans l’évaluation des risques sanitaires et environnementaux de sols contaminés},

author = {Benjamin Pauget and Maxime Louzon and Aurélie Pelfrêne and Frédéric Gimbert and Francis Douay and Annette De-Vaufleury},

year  = {2019},

date = {2019-01-01},

booktitle = {4ème Rencontres nationales de la Recherche sur les sites et sols pollués, Le Beffroi de Montrouge, 26-27 novembre 2019, Paris},

note = {ACTN},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Pelfrene2019f,

title = {Une méthode simplifiée pour estimer la bioaccessibilité des polluants métalliques dans les sols},

author = {Aurélie Pelfrêne and Barbara Le-Bot and Christophe Waterlot and Philippe Glorennec and Karin Sahmer and Francis Douay},

year  = {2019},

date = {2019-01-01},

booktitle = {4ème Rencontres nationales de la Recherche sur les sites et sols pollués, Le Beffroi de Montrouge, 26-27 novembre 2019, Paris},

note = {ACTN},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Waterlot2019b,

title = {Benefits of Ryegrass on Multicontaminated Soils Part 2: Green process to provide Idrocilamide},

author = {Christophe Waterlot and Pierrick Dufrénoy and Marie Hechelski and Brice Louvel and Adam Daïch and Alina Ghinet},

year  = {2019},

date = {2019-01-01},

journal = {Sustainability},

volume = {11},

number = {23},

pages = {6685-6694},

abstract = {A restoration of highly contaminated garden soil is proposed as a 

	greener alternative to the production of vegetables. Depending on 

	potentially toxic elements and their concentration, ryegrass shoots 

	accumulate these elements in sufficient quantity to be used as a 

	catalyst in organic synthesis. The analysis of Cd, Pb, Zn, Cu, Fe, 

	Mn, and Al in ashes issued from the calcination of ryegrass shoots 

	revealed that the concentration of Zn was highest (>7000 mg kg–1). 

	The ratios between potential Lewis acids (Zn, Cu, Fe, Mn, and Al) 

	to carcinogenic metals (Cd or Pb) were 191 ± 7 for LA/Cd and 235 

	± 13 for LA/Pb, making the shoots of ryegrass suitable for the production 

	of Zn-rich polymetallic biosourced catalysts. This material was used 

	in the synthesis of idrocilamide under free-solvent condition, providing 

	the drug in a good yield (69.9%). Data show that a limitation of 

	waste, a maximization of the material incorporation in the process, 

	a minimization of the steps, and an optimization of the stoichiometric 

	factor are the main innovative factors in the current process in 

	comparison with those previously reported.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Courtois2019,

title = {Ecotoxicology of silver nanoparticles and their derivatives introduced in soil with or without sewage sludge: A review of effects on microorganisms, plants and animals},

author = {Pauline Courtois and Agnieszka Rorat and Sébastien Lemiere and Remy Guyoneaud and Eleonore Attard and Clément Levard and Franck Vandenbulcke},

year  = {2019},

date = {2019-01-01},

journal = {Environmental Pollution},

volume = {253},

pages = {578-598},

abstract = {Silver nanoparticles (AgNPs) are widely incorporated in many products, 

	partly due to their antimicrobial properties. The subsequent discharge 

	of this form of silver into wastewater leads to an accumulation of 

	silver species (AgNPs and derivatives resulting from their chemical 

	transformation), in sewage sludge. As a result of the land application 

	of sewage sludge for agricultural or remediation purposes, soils 

	are the primary receiver media of silver contamination. Research 

	on the long-term impact of AgNPs on the environment is ongoing, and 

	this paper is the first review that summarizes the existing state 

	of scientific knowledge on the potential impact of silver species 

	introduced into the soil via sewage sludge, from microorganisms to 

	earthworms and plants. Silver species can easily enter cells through 

	biological membranes and affect the physiology of organisms, resulting 

	in toxic effects. In soils, exposure to AgNPs may change microbial 

	biomass and diversity, decrease plant growth and inhibit soil invertebrate 

	reproduction. Physiological, biochemical and molecular effects have 

	been documented in various soil organisms and microorganisms. Negative 

	effects on organisms of the dominant form of silver in sewage sludge, 

	silver sulfide (Ag2S), have been observed, although these effects 

	are attenuated compared to the effects of metallic AgNPs. However, 

	silver toxicity is complex to evaluate and much remains unknown about 

	the ecotoxicology of silver species in soils, especially with respect 

	to the possibility of transfer along the trophic chain via accumulation 

	in plant and animal tissues. Critical points related to the hazards 

	associated with the presence of silver species in the environment 

	are described, and important issues concerning the ecotoxicity of 

	sewage sludge applied to soil are discussed to highlight gaps in 

	existing scientific knowledge and essential research directions for 

	improving risk assessment. (C) 2019 Elsevier Ltd. All rights reserved.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Dubois2019,

title = {Reconstitution de sculptures fragmentées par approches multitechniques (micropaléontologie, pétrographie et minéralogie) : exemple du gisant de Jeanne d’Ecaussinnes du Musée de Valenciennes},

author = {Michel Dubois and Eric Armynot-du-Châtelet and V Hadot and R Toullec and Sandra Ventalon},

year  = {2019},

date = {2019-01-01},

booktitle = {Rencontres Interdisciplinaires et Interprofessionnelles Géosciences - Archéologie, 17-18 juin 2019, Strasbourg},

pages = {15-16},

note = {AFF},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Dupont2019,

title = {Genetic variation of the epigeic earthworm Lumbricus castaneus populations in urban soils of the Paris region (France) revealed using eight newly developed microsatellite markers},

author = {Lise Dupont and Maxime Pauwels and Cassandre Dume and Valentin Deschins and Hélène Audusseau and Agnès Gigon and Florence Dubs and Franck Vandenbulcke},

year  = {2019},

date = {2019-01-01},

journal = {Applied Soil Ecology},

volume = {135},

pages = {33-37},

abstract = {Urban soils are subject to intense environmental pressures, e.g. physical 

	disturbance, surface transformation and pollution, which greatly 

	impact the activity of soil organisms. The epigeic earthworm Lumbricus 

	castaneus (Savigny, 1826) is known to be tolerant to trace elements 

	contamination of soils and was found to be abundant in the urban 

	context. In order to investigate how urban environmental stressors 

	shape the population genetic variation of this species, we developed 

	8 microsatellite loci from a microsatellite-enriched genomic library. 

	Polymorphism was explored in 6 populations (153 individuals) from 

	the Paris region. The number of alleles per locus varied from 7 to 

	29. The average expected heterozygosity within populations ranged 

	from 0.596 to 0.705. These new microsatellite markers revealed a 

	significant genetic structure at fine spatial scale, highlighting 

	that they could be particularly useful for genetic studies of populations 

	in urban soils.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Dusseaux2019,

title = {Characterization of meteoric water infiltration in Variscan shear zones},

author = {Camille Dusséaux and Aude Gebelin and Philippe Boulvais and Véronique Gardien and Michel Dubois and G Ruffet and Marc Poujol and Y Branquet and S Grimes and Andreas Mulch},

year  = {2019},

date = {2019-01-01},

booktitle = {Goldschmidt Conference, 18-13 August 2019, Barcelone (Espagne)},

note = {COM},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Gauthier2019,

title = {Apport de la minéralogie et de la géochimie à l’étude de la compréhension des processus archéométallurgiques. Cas des ferriers de la forêt de Moulière},

author = {Arnaud Gauthier and Michel Dubois and L Cochard},

year  = {2019},

date = {2019-01-01},

booktitle = {Rencontres Interdisciplinaires et Interprofessionnelles Géosciences - Archéologie, 17 -18 juin 2019, Strasbourg},

pages = {29-30},

note = {AFF},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Jaskulak2019,

title = {Gene expression, DNA damage and other stress markers in Sinapis alba L. exposed to heavy metals with special reference to sewage sludge application on contaminated sites},

author = {Marta Jaskulak and Anna Grobelak and Anna Grosser and Franck Vandenbulcke},

year  = {2019},

date = {2019-01-01},

journal = {Ecotoxicology and Environmental Safety},

volume = {181},

pages = {508-517},

abstract = {Bioindicators are promising tools used to detect the long-term effects 

	of selected biosolids on plants development and should be implemented 

	before large-scale supplementation of sewage sludge into the soil. 

	The presented study shows the impact of sewage sludge application 

	on metal-sensitive toxicity biological parameters (biomarkers) in 

	Sinapis alba including: germination, root length, the activity of 

	guaiacol peroxidase, the chlorophyll content, the level of DNA damage 

	and the expression level of Ribulose-1,5-bisphosphate carboxylase/oxygenase 

	(rbcL) and metallothionein (mt). We evaluated data from selected 

	biomarkers in order to broaden our understanding of plants defense 

	mechanisms against heavy metal contamination and the application 

	of sewage sludge into soils. Overall, in contaminated soil after 

	supplementation with both municipal sewage sludges, an increase in 

	toxicity was noticed in DNA damage, mt and rbcl expression and total 

	chlorophyll content. The supplementation of both soils with municipal 

	sewage sludge caused a two-time induction in the mt expression. Moreover, 

	clean soil supplemented with sewage sludge caused an increase in 

	DNA damage shown as the tail moment from approximately 12 mu m on 

	control to 40 mu m after supplementation. Even if those biosolids 

	increased the initial germination, roots length, and biomass in comparison 

	to the unamended soil, the toxicity was evidenced with other stress 

	markers. Results showed, that in order to accurately assess the influence 

	of sewage sludge application on plants the use of several specific 

	biomarkers is required for safe land restoration. The conducted study 

	also confirmed, both under biochemical and genotoxic tests, that 

	iron enrichment for biosolids or contaminated soil can significantly 

	reduce the bioavailability and toxicity of other metals.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Jaskulak2019a,

title = {Bioaccumulation, antioxidative response, and metallothionein expression in Lupinus luteus L. exposed to heavy metals and silver nanoparticles},

author = {Marta Jaskulak and Agnieszka Rorat and Anna Grobelak and Zayneb Chaabene and Malgorzata Kacprzak and Franck Vandenbulcke},

year  = {2019},

date = {2019-01-01},

journal = {Environmental Science and Pollution Research},

volume = {26},

number = {16},

pages = {16040-16052},

abstract = {Yellow-lupin (Lupinus luteus L.) was grown on soils contaminated with 

	heavy metals during two parallel studies. In the first one, the soil 

	was contaminated by industrial activities whereas, in the second 

	one, the soil was artificially contaminated with a single metal including 

	Cd, Pb, Zn, Ni (in nitrate form), and Ag (in nitrate and nanoparticles 

	form). The study was performed to assess a plant's response to contamination 

	including its antioxidative response and molecular mechanisms involved 

	in metal detoxification through the expression level of metallothioneins 

	(MTs). Overall, the study provided insights into identification and 

	validation of housekeeping genes (HKG) in L. luteus under exposure 

	to metal stress and showed the effects of selected heavy metals and 

	silver nanoparticles on the expression of metallothioneins, the activity 

	of guaiacol peroxidase (GPX) and bioaccumulation of metals in leaves 

	of L. luteus. As such, HKG validation using BestKeeper, NormFinder, 

	and geNorm software allowed for the selection of four most stable 

	reference genes in a context metal contamination for the selected 

	plant. Moreover, a significant increase in the expression levels 

	of MT was observed in plants grown under heavy metal stress and none 

	on plants grown on 25mgkg(-1) of silver nanoparticles. Also, the 

	GPX activity and MT expression showed statistically significant changes 

	between different conditions and doses which means that they can 

	be used as highly sensitive stress markers for planning the phytoremediation 

	process on a large scale.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Verron2019,

title = {Experimental Study of Pyrite Oxidation at 100 degrees C: Implications for Deep Geological Radwaste Repository in Claystone},

author = {Héloïse Verron and Jérôme Sterpenich and Julien Bonnet and Franck Bourdelle and Régine Mosser-Ruck and Catherine Lorgeoux and Aurélien Randi and Nicolas Michau},

year  = {2019},

date = {2019-01-01},

journal = {Minerals},

volume = {9},

number = {7},

pages = {16},

abstract = {The oxidation of pyrite is one of the near field processes of the 

	chemical evolution of clay rock planned to host a deep geological 

	radioactive waste repository during operation. Indeed, this process 

	can lead to transitory acidic conditions in the medium (i.e., production 

	of sulphuric acid, carbonic acid) which may influence the corrosion 

	kinetics of the carbon steel components of some disposal cells. In 

	order to improve the geochemical modelling of the long-term disposal, 

	the oxidation of pyrite in contact with clays and carbonates at 100 

	degrees C must be evaluated. In this study, special attention was 

	paid to the pyrite oxidation rate thanks to an original experimental 

	set-up, involving several pyrite/mineral mixtures and a reactor coupled 

	to a micro gas chromatograph (P-O2 and P-CO2 monitoring). Although 

	thermodynamic modelling expects that hematite is the most stable 

	phase in a pure pyrite heated system (low pH), experiments show the 

	formation of native sulfur as an intermediate product of the reaction. 

	In the presence of calcite, the pH is neutralized and drives the 

	lower reactivity of pyrite in the absence of native sulfur. The addition 

	of clay phases or other detrital silicates from the claystone had 

	no impact on pyrite oxidation rate. The discrepancies between experiments 

	and thermodynamic modelling are explained by kinetic effects. Two 

	laws were deduced at 100 degrees C. The first concerns a pure pyrite system, with the following law: rPy= 10-4.8 center dot PO20.5 center dot t-0.5. The second concerns a pyrite/carbonates system: rPy+Ca= 

	10-5.1 center dot PO20.5 center dot t-0.5 where P-O2 corresponds 

	to the partial pressure of O-2 (in bar) and t is time in seconds. 

	Different mechanisms are proposed to explain the evolution with time 

	of the O-2 consumption during pyrite oxidation: (i) decrease of the 

	specific or reactive surface area after oxidation of fine grains 

	of pyrite, (ii) decrease of O-2 pressure, (iii) growing up of secondary 

	minerals (Fe-oxides or anhydrite in the presence of calcium in the 

	system) on the surface of pyrite limiting the access of O-2 to the 

	fresh surface of pyrite, and (iv) change in the pH of the solution.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@techreport{Chalmandrier2019,

title = {Retour d’expérience sur la mise en place d’une expérimentation sur une friche péri-urbaine contaminée : dépasser la notion «d’acceptabilité sociale» pour faire du sol un bien commun},

author = {Chalmandrier Maud and Douay Francis and Louvel Brice and Petit-Berghem Yves and Rémy Elisabeth},

year  = {2019},

date = {2019-01-01},

urldate = {2019-01-01},

note = {VAL},

keywords = {ER4},

pubstate = {published},

tppubtype = {techreport}

}

@conference{Charvet2019,

title = {Introduction aux débats relatifs aux sols urbains et périurbains},

author = {Charvet Ronald and Douay Francis and Rémy Elisabeth},

year  = {2019},

date = {2019-01-01},

urldate = {2019-01-01},

booktitle = {Journée d'échanges "Qualité des sols urbains et périurbains et compatibilité des usages", OSUNA, 13 mai 2019, Paris},

abstract = {• Des sols urbains et périurbains : des milieux complexes et méconnus 

	• Disparité des connaissances sur ces sols à l’échelle du territoire 

	• Des inventaires historiques urbains disponibles sur certaines villes, 

	quelle échelle temporelle prendre en compte pour analyser la dialectique 

	Santé-Environnement ? • Nécessité de connaître les trajectoires socio-historiques, 

	agronomiques et physico-chimiques des sols pour prendre en compte 

	les héritages complexes qui les ont façonnés Une diversité de situations 

	et d’attentes • Des modalités de gestion différentes entre jardins 

	existants et jardins en création • En cas de contamination des sols 

	: des responsabilités partagées pour collectivités, associations, 

	experts • les leviers possibles pour reconsidérer des situations 

	problématiques ? • Instaurer des valeurs-seuils sur les sols ne semble 

	pas une priorité des pouvoirs publics. Aujourd’hui on réalise l’analyse 

	de l’exposition des populations au cas par cas : nécessité de suivis, 

	de contrôles, accompagnement des jardiniers dans leurs pratiques 

	sur le moyen ou long terme • Le sol : un patrimoine à préserver et 

	un bien commun. Faire reconnaître le rôle des jardiniers comme rendant 

	des services d’intérêt général},

note = {COM},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{PALUCH2019364,

title = {High airborne level of Aspergillus fumigatus and presence of azole-resistant TR34/L98H isolates in the home of a cystic fibrosis patient harbouring chronic colonisation with azole-resistant H285Y A. fumigatus},

author = {M. Paluch and S. Lejeune and E. Hecquet and A. Prévotat and A. Deschildre and Emilie Fréalle},

url = {https://www.sciencedirect.com/science/article/pii/S1569199319300013},

doi = {https://doi.org/10.1016/j.jcf.2019.01.001},

issn = {1569-1993},

year  = {2019},

date = {2019-01-01},

urldate = {2019-01-01},

journal = {Journal of Cystic Fibrosis},

volume = {18},

number = {3},

pages = {364-367},

abstract = {Azole-resistant Aspergillus fumigatus (ARAF) has been reported in the domestic environment of patients at risk for aspergillosis. Here, we assessed the mother's and father's homes of an 18-year-old cystic fibrosis patient harbouring chronic colonisation with H285Y CYP51A azole-resistant isolate, in order to explore the link between environmental exposure and ARAF infection. In one dwelling, a very high overall contamination level was found (710–7.240 CFU/m3), with a predominance of A. fumigatus (640–6.490 CFU/m3), and ARAF showing the TR34/L98H mutation was isolated. Mycological follow-up of the patient showed the persistence of H285Y isolates, but no acquisition of TR34/L98H isolates was observed. This could be due to the low proportion of TR34/L98H isolates (<3%), or the establishment of preventative measures and dwelling remediation taken after the environmental investigation. Our data underlines the value of an environmental assessment to establish preventative measures and limit the risk of A. fumigatus exposure and ARAF acquisition.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{allami:hal-04288648,

title = {Amendment-assisted revegetation of mine tailings: improvement of tailings quality and biomass production},

author = {Mariam Al-Lami and Nadège Oustrière and Eva Gonzales and Joel Burken},

url = {https://hal.science/hal-04288648},

doi = {10.1080/15226514.2018.1537249},

year  = {2019},

date = {2019-04-01},

urldate = {2019-04-01},

journal = {International Journal of Phytoremediation},

volume = {21},

number = {5},

pages = {425-434},

publisher = {Taylor & Francis},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Chaabene2018,

title = {Copper toxicity and date palm (Phoenix dactylifera) seedling tolerance: Monitoring of related biomarker},

author = {Zayneb Chaâbene and Imen Rekik-Hakim and Agnieszka Rorat and Amine Elleuch and Hafedh Mejdoub and Franck Vandenbulcke},

year  = {2018},

date = {2018-01-01},

journal = {Environmental Toxicology and Chemistry},

volume = {Article in Press. DOI: 10.1002/etc.4007},

abstract = {Date palm (Phoenix dactylifera) seeds were exposed to different copper (Cu) solutions to examine plant stress responses. Low Cu concentrations (0.02 and 0.2 mM) caused an increase of seed germination, whereas higher Cu amounts (2 mM) significantly inhibited seed germination, delayed hypocotyl elongation, increased seedling mortality, and reduced the germination index by more than 90%. Metal-related toxicity symptoms appeared after 15 d of 2 mM of Cu exposure. Biochemical activities such as amylase activity and redox balance elements were examined to study the relationship between external Cu amount and internal plant response. The present study showed that amylolytic activity	was dose- and time-dependent. Likewise, H2 O2 production increased after exposure to Cu, which was correlated with thiobarbituric acid reactive substance (TBARS) accumulation. Furthermore at low Cu concentrations, superoxide dismutase (SOD) and catalase (CAT) activities increased, suggesting that date palm seed stimulated its metal homeostasis networks.	However, the highest cupric ion amounts increased cell oxidant accumulation and reduced enzyme production. Gene expression level measures of P. dactylifera phytochelatin synthase (Pdpcs) and P. dactylifera metallothionein (Pdmt) encoding genes have been carried out to investigate the implication of PdPCS and PdMT proteins in Cu homeostasis and/or	its sequestration. Phoenix dactylifera metallothionein induction reached a peak after 30 d of exposure to 0.2 mM of Cu. However, it was down-regulated in plants exposed to higher Cu concentrations. In the same conditions, Pdpcs was overexpressed during 1 mo of exposure before it decreased thereafter. These observations provide a new insight into date palm cell response to Cu, a metal that can be toxic but that is also an essential element.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Chaabene2018a,

title = {Insight into the expression variation of metal-responsive genes in the seedling of date palm (Phoenix dactylifera)},

author = {Zayneb Chaâbene and Agnieszka Rorat and Imen Rekik-Hakim and Fabien Bernard and Grubb-C. Douglas and Amine Elleuch and Franck Vandenbulcke and Hafedh Mejdoub},

year  = {2018},

date = {2018-01-01},

journal = {Chemosphere},

volume = {197},

pages = {123-134},

abstract = {Phytochelatin synthase and metallothionein gene expressions were monitored via qPCR in order to investigate the molecular mechanisms involved in Cd and Cr detoxification in date palm (Phoenix dactylifera). A specific reference gene validation procedure using BestKeeper, NormFinder and geNorm programs allowed selection of the three most stable reference genes in a context of Cd or Cr contamination among six reference gene candidates, namely elongation factor α1, actin, aldehyde dehydrogenase, SAND family, tubulin 6 and TaTa box binding protein. Phytochelatin synthase (pcs) and metallothionein (mt) encoding gene expression were induced from the first days of exposure. At low Cd stress (0.02mM), genes were still up- regulated until 60th day of exposure. At the highest metal concentrations, however, pcs and mt gene expressions decreased. pcs encoding gene was significantly up-regulated under Cr exposure, and was more responsive to increasing Cr concentration than mt encoding gene. Moreover, exposure to Cd or Cr influenced clearly seed germination and hypocotyls elongation. Thus, the results have proved that both analyzed genes participate in metal detoxification and their expression is regulated at transcriptional level in date palm subjected to Cr and Cd stress. Consequently, variations of expression of mt and pcs genes may serve as early-warning biomarkers of metal stress in this species.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Crognier2018,

title = {Syn-orogenic fluid flow in the Jaca basin (South Pyrenean Fold and Thrust Belt) from fracture and vein analyses},

author = {Nemo Crognier and Guilhem Hoareau and Charles Aubourg and Michel Dubois and Brice Lacroix and Mathieu Branellec and Jean-Paul Callot and Torsten Vennemann},

year  = {2018},

date = {2018-01-01},

journal = {Basin Research},

pages = {Accepted manuscript online},

abstract = {This study aims at understanding the origin and nature of syn-orogenic fluid flow in the Jaca basin from the South Pyrenean fold and thrust-belt, as recorded in calcite and quartz veins of the Sierras Interiores (Spain) and the turbiditic basin, which cover upper Cretaceous t Late Eocene syntectonic deposits. The fracture network consists of	a classical pattern of transverse and longitudinal fractures related to Layer Parallel Shortening (LPS) and folding respectively. Veins filled equally about the third of fractures in the carbonate shelf	and turbidites. Carbon and oxygen isotopes of calcite veins mostly indicate precipitation from isotopically buffered water, consistent with high water-rock interaction. In the Sierras Interiores, petrographical observations and fluid inclusion microthermometry are consistent with two distinct stages of precipitation. The first stage is characterized by relatively low Th and low salinities (155-205° C and 0.5-3.2 wt% eq. NaCl). The second stage, which was characterized both by the	formation of mode-I joints and by mode-I reactivation of preexisting	veins, shows higher Th and salinities (215-270°C and 2.2-5.7 wt%	eq. NaCl). Waters recorded in the second stage are interpreted to have interacted with underlying Triassic evaporites and flowed along major thrusts before vein precipitation, which are locally in thermal disequilibrium with host-rocks. We suggest the transition from a 

rather closed hydrological system during the first stage of vein formation, interpreted to have occurred during Eaux-chaudes thrusting	(upper Lutetian-Bartonian), to a more open hydrological system during the second stage, which likely occurred during Gavarnie thrusting (Priabonian-early Rupelian). Finally, we also document the migration in space and time of hydrothermal pulses along the South Pyrenean Foreland Basin, related to the westward propagation of major thrusts during the Pyrenean orogeny.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Leclercq-Dransart2018,

title = {Litter breakdown as a tool for assessment of the efficiency of afforestation and ash-aided phytostabilisation on metal contaminated soils functioning in Northern France},

author = {Julie Leclercq-Dransart and Lucia Santorufo and Céline Pernin and Brice Louvel and Sylvain Demuynck and Fabien Grumiaux and Francis Douay and Alain Leprêtre},

year  = {2018},

date = {2018-01-01},

journal = {Environmental Science and Pollution Research},

abstract = {The main objective of the study was to assess the efficiency of phytoremediation methods implemented for 14 years on highly metal contaminated soils. The different experimental strategies were plots planted with a tree mix or with a single tree species coupled or not with the use of fly-ashes as an amendment to limit metals mobility in soil. The breakdown of poplar litter on the four plots was monitored during 10 months. In parallel, colonization of litter bags by functional groups of mesofauna (Collembola and Acari) was followed. Two mesh sized litter-bags were used to allow distinguishing microbial and mesofaunal actions	on the litter breakdown. We observed the breakdown of litter in four 

studied plots. Litter breakdown occurred faster in 3 mm litter bags than 250 µm ones during summer demonstrating the importance of mesofauna. Mixed plantation allowed faster litter breakdown than mono-specific plantation. A higher abundance of mesofauna and/or better abiotic conditions (moisture, shading…) could explain this result. Regarding litter breakdown and mesofauna, no significant difference was observed between the amended plots and those subjected to soil phytomanagement.	However, communities of the studied area are disturbed since a low abundance of detritivores was observed. This could explain also the slower litter breakdown than expected in our study. To conclude, 

among the phytomanagement methods tested, mixed plantations could provide a benefit for the restoration of degraded soils. By contrast, the use of fly-ashes does not seem to have any effect on the functionality	of ecosystem neither on the litter breakdown process nor on the abundance of mesofauna.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Lomaglio2018,

title = {Cd, Pb, and Zn mobility and (bio)availability in contaminated soils from a former smelting site amended with biochar},

author = {Tonia Lomaglio and Nour Hattab-Hambli and Florie Miard and Manhattan Lebrun and Romain Nandillon and Dalila Trupiano and Gabriella-Stefania Scippa and Arnaud Gauthier and Mikael Motelica-Heino and Sylvain Bourgerie and Domenico Morabito},

year  = {2018},

date = {2018-01-01},

journal = {Environmental Science and Pollution Research},

abstract = {Biochar is a potential candidate for the remediation of metal(loid)-contaminated soils. However, the mechanisms of contaminant-biochar retention and release depend on the amount of soil contaminants and physicochemical characteristics, as well as the durability of the biochar contaminant complex, which may be related to the pyrolysis process parameters. The objective of the present study was to evaluate, in a former contaminated smelting site, the impact of two doses of wood biochar (2 and 5% w/w) on metal immobilization and/or phytoavailability and their effectiveness in promoting plant growth in mesocosm experiments. Different soil mixtures were investigated. The main physicochemical parameters and the Cd, Pb, and Zn contents were determined in soil and in soil pore	water. Additionally, the growth, dry weight, and metal concentrations	were analyzed in the different dwarf bean plant (Phaseolus vulgaris L.) organs tested. Results showed that the addition of biochar at two doses (2 and 5%) improved soil conditions by increasing soil pH, electrical conductivity, and water holding capacity. Furthermore, the application of biochar (5%) to metal-contaminated soil reduced	Cd, Pb, and Zn mobility and availability, and hence their accumulation in the different P. vulgaris L. organs. In conclusion, the data clearly demonstrated that biochar application can be effectively used for Cd, Pb, and Zn immobilization, thereby reducing their bioavailability and phytotoxicity.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Lors2018,

title = {Environmental hazard assessment by the Ecoscore system to discriminate PAH-polluted soils},

author = {Christine Lors and Jean-François Ponge and Denis Damidot},

year  = {2018},

date = {2018-01-01},

journal = {Environmental Science and Pollution Research},

abstract = {A bioassay battery-integrated index was applied to different soils sampled from a former coke factory, with the aim to evaluate the discriminating capacity of the Ecoscore system (ES) to assess the environmental hazard of PAH-polluted soils. Two soils from a former coke factory, polluted with polycyclic aromatic hydrocarbons (PAHs), were evaluated for their ecotoxicity to terrestrial and aquatic organisms and their genotoxicity. These soils have been already presented in	a previous paper but data have been reanalyzed for the present article in an endeavor to standardize the ES. One soil was sampled in the untreated site and the second underwent a windrow treatment. While these soils had a similar total concentrations of US-EPA 16PAHs (around 3000 mg kg−1), different ecoscores were obtained when subjected to a set of solid- and liquid-phase bioassays measuring acute, chronic, and genotoxic effects. The total PAH content of the soil is not a pertinent parameter to assess soil pollution hazards contrary to the ES. ES is a robust method to classify soils according to their toxicity level. Four levels of toxicity have been defined: no (ecoscore = 0), weak (0 < ecoscore ≤33), moderate (33 < ecoscore ≤67), and	strong toxicity (67 < ecoscore ≤ 100). The combination of chemical and toxicological data highlights the relationship between three-ring PAHs and acute ecotoxicity. Conversely, chronic effects of water extracts on algal growth could be explained by high molecular weight PAHs, such as five- and six-ring PAHs.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}