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Auteurs : Leclercq-Dransar Juliet; Santorufo Lucia; Pernin Céline; Louvel Brice; Demuynck Sylvain; Grumiaux Fabien; Douay Francis; Leprêtre Alain Litter breakdown as a tool for assessment of the efficiency of afforestation and ash-aided phytostabilisation on metal contaminated soils functioning in Northern France Environmental Science and Pollution Research, @article{Leclercq-Dransart2018, title = {Litter breakdown as a tool for assessment of the efficiency of afforestation and ash-aided phytostabilisation on metal contaminated soils functioning in Northern France}, author = {Juliet Leclercq-Dransar and Lucia Santorufo and Céline Pernin and Brice Louvel and Sylvain Demuynck and Fabien Grumiaux and Francis Douay and Alain Leprêtre}, year = {2018}, date = {2018-01-01}, journal = {Environmental Science and Pollution Research}, abstract = {The main objective of the study was to assess the efficiency of phytoremediation methods implemented for 14 years on highly metal contaminated soils. The different experimental strategies were plots planted with a tree mix or with a single tree species coupled or not with the use of fly-ashes as an amendment to limit metals mobility in soil. The breakdown of poplar litter on the four plots was monitored during 10 months. In parallel, colonization of litter bags by functional groups of mesofauna (Collembola and Acari) was followed. Two mesh sized litter-bags were used to allow distinguishing microbial and mesofaunal actions on the litter breakdown. We observed the breakdown of litter in four studied plots. Litter breakdown occurred faster in 3 mm litter bags than 250 µm ones during summer demonstrating the importance of mesofauna. Mixed plantation allowed faster litter breakdown than mono-specific plantation. A higher abundance of mesofauna and/or better abiotic conditions (moisture, shading…) could explain this result. Regarding litter breakdown and mesofauna, no significant difference was observed between the amended plots and those subjected to soil phytomanagement. However, communities of the studied area are disturbed since a low abundance of detritivores was observed. This could explain also the slower litter breakdown than expected in our study. To conclude, among the phytomanagement methods tested, mixed plantations could provide a benefit for the restoration of degraded soils. By contrast, the use of fly-ashes does not seem to have any effect on the functionality of ecosystem neither on the litter breakdown process nor on the abundance of mesofauna.}, note = {ACL}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {article} } The main objective of the study was to assess the efficiency of phytoremediation methods implemented for 14 years on highly metal contaminated soils. The different experimental strategies were plots planted with a tree mix or with a single tree species coupled or not with the use of fly-ashes as an amendment to limit metals mobility in soil. The breakdown of poplar litter on the four plots was monitored during 10 months. In parallel, colonization of litter bags by functional groups of mesofauna (Collembola and Acari) was followed. Two mesh sized litter-bags were used to allow distinguishing microbial and mesofaunal actions on the litter breakdown. We observed the breakdown of litter in four studied plots. Litter breakdown occurred faster in 3 mm litter bags than 250 µm ones during summer demonstrating the importance of mesofauna. Mixed plantation allowed faster litter breakdown than mono-specific plantation. A higher abundance of mesofauna and/or better abiotic conditions (moisture, shading…) could explain this result. Regarding litter breakdown and mesofauna, no significant difference was observed between the amended plots and those subjected to soil phytomanagement. However, communities of the studied area are disturbed since a low abundance of detritivores was observed. This could explain also the slower litter breakdown than expected in our study. To conclude, among the phytomanagement methods tested, mixed plantations could provide a benefit for the restoration of degraded soils. By contrast, the use of fly-ashes does not seem to have any effect on the functionality of ecosystem neither on the litter breakdown process nor on the abundance of mesofauna. |
Auteurs : ADEME ; Bert Valérie; Douay Francis; Faure Olivier; Cadière Frédérique Les phytotechnologies appliquées aux sites et sols pollués (nouveaux résultats de recherche et démonstration) ADEME @techreport{ADEME2017, title = {Les phytotechnologies appliquées aux sites et sols pollués (nouveaux résultats de recherche et démonstration)}, author = {ADEME and Valérie Bert and Francis Douay and Olivier Faure and Frédérique Cadière}, year = {2017}, date = {2017-01-01}, institution = {ADEME}, abstract = {Les phytotechnologies appliquées à la gestion des sites pollués regroupent un ensemble de techniques qui utilisent in situ des espèces végétales pour contenir, extraire ou dégrader des polluants inorganiques ou organiques du sol. Ces techniques, bien que connues et relativement bien perçues par les gestionnaires et opérateurs des sites pollués, restent encore émergentes sur les marchés des techniques de gestion des sites et sols pollués, notamment par manque de recul opérationnel et d’informations sur les possibilités de valorisation de la biomasse produite et les coûts et bénéfices associés. Cet ouvrage présente de nouveaux résultats de recherche et de démonstration issues de projets encouragés ou soutenus par l’ADEME et finalisés depuis 2012. A ce titre, il complète et enrichit le contenu du guide « Les phytotechnologies appliquées aux sites et sols pollués : Etat de l’art et guide de mise en oeuvre » réalisé par l’ADEME et l’INERIS. L’approche globale de gestion des sites et sols pollués (phytomanagement) est envisagée au travers de 5 chapitres dédiés à la présentation d’outils d’aide à la décision (Chapitre 1), de retours d’expériences et d’exemples d’application de phytostabilisation (Chapitre 2), d’outils d’estimation des transferts et des expositions des écosystèmes en lien avec l’évaluation de la biodisponibilité des polluants (Chapitre 3), de filières de conversion de la biomasse en énergie et de leurs enjeux sociaux, règlementaires (Chapitre 4) et économiques (Chapitre 5). La synthèse des outils disponibles d’aide à la décision et les informations à caractères techniques, économiques, règlementaires et sociaux présentés dans cet ouvrage doivent permettre à tout utilisateur ou prescripteur potentiel des phytotechnologies de valider la pertinence de l’utilisation des phytotechnologies au regard des objectifs de gestion du site.}, note = {OV}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {techreport} } Les phytotechnologies appliquées à la gestion des sites pollués regroupent un ensemble de techniques qui utilisent in situ des espèces végétales pour contenir, extraire ou dégrader des polluants inorganiques ou organiques du sol. Ces techniques, bien que connues et relativement bien perçues par les gestionnaires et opérateurs des sites pollués, restent encore émergentes sur les marchés des techniques de gestion des sites et sols pollués, notamment par manque de recul opérationnel et d’informations sur les possibilités de valorisation de la biomasse produite et les coûts et bénéfices associés. Cet ouvrage présente de nouveaux résultats de recherche et de démonstration issues de projets encouragés ou soutenus par l’ADEME et finalisés depuis 2012. A ce titre, il complète et enrichit le contenu du guide « Les phytotechnologies appliquées aux sites et sols pollués : Etat de l’art et guide de mise en oeuvre » réalisé par l’ADEME et l’INERIS. L’approche globale de gestion des sites et sols pollués (phytomanagement) est envisagée au travers de 5 chapitres dédiés à la présentation d’outils d’aide à la décision (Chapitre 1), de retours d’expériences et d’exemples d’application de phytostabilisation (Chapitre 2), d’outils d’estimation des transferts et des expositions des écosystèmes en lien avec l’évaluation de la biodisponibilité des polluants (Chapitre 3), de filières de conversion de la biomasse en énergie et de leurs enjeux sociaux, règlementaires (Chapitre 4) et économiques (Chapitre 5). La synthèse des outils disponibles d’aide à la décision et les informations à caractères techniques, économiques, règlementaires et sociaux présentés dans cet ouvrage doivent permettre à tout utilisateur ou prescripteur potentiel des phytotechnologies de valider la pertinence de l’utilisation des phytotechnologies au regard des objectifs de gestion du site. |
Auteurs : Al-Souki Karim; Louvel Brice; Douay Francis; Pourrut Bertrand Interest of Miscanthus x Giganteus in phytomanaging heavy metal contaminated soils 2nd World Congress on Civil, Structural, and Environmental Engineering (CSEE’17), 2-4 April 2017, Barcelona (Espagne) @conference{Al-Souki2017a, title = {Interest of Miscanthus x Giganteus in phytomanaging heavy metal contaminated soils}, author = {Karim Al-Souki and Brice Louvel and Francis Douay and Bertrand Pourrut}, year = {2017}, date = {2017-01-01}, booktitle = {2nd World Congress on Civil, Structural, and Environmental Engineering (CSEE’17), 2-4 April 2017, Barcelona (Espagne)}, note = {COM}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } |
Auteurs : Al-Souki Karim-Suhail; Louvel Brice; Douay Francis; Pourrut Bertrand Assessment of Miscanthus x giganteus capacity to restore the functionality of metal-contaminated soils: Ex situ experiment Applied Soil Ecology, 115, pp. 44-521 @article{Al-Souki2017c, title = {Assessment of Miscanthus x giganteus capacity to restore the functionality of metal-contaminated soils: Ex situ experiment}, author = {Karim-Suhail Al-Souki and Brice Louvel and Francis Douay and Bertrand Pourrut}, year = {2017}, date = {2017-01-01}, journal = {Applied Soil Ecology}, volume = {115}, pages = {44-52}, abstract = {Phytostabilization can be an appropriate choice for managing soils contaminated by diverse metals. Miscanthus x giganteus was selected to phytostabilize polluted soils surrounding the former lead smelter Metaleurop Nord, in Northern France. The aim of the current study was to determine the impact of miscanthus plants on restoring the corresponding soil functionality. Ex situ experiments revealed that soil biological activities (basal respiration, fluorescein diacetate hydrolytic activity, acid phosphatases, laccases and ureases) as well as microbial biomass carbon increased in contaminated soils cultivated with miscanthus, compared to unplanted soils. These results verify the capacity of miscanthus to restore polluted soil functionalities, indicating the positive effect of phytostabilization on soil biological activities. In conclusion, revegetating polluted soils with miscanthus plants can restore their capacity to perform biochemically and biologically.}, note = {ACL}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {article} } Phytostabilization can be an appropriate choice for managing soils contaminated by diverse metals. Miscanthus x giganteus was selected to phytostabilize polluted soils surrounding the former lead smelter Metaleurop Nord, in Northern France. The aim of the current study was to determine the impact of miscanthus plants on restoring the corresponding soil functionality. Ex situ experiments revealed that soil biological activities (basal respiration, fluorescein diacetate hydrolytic activity, acid phosphatases, laccases and ureases) as well as microbial biomass carbon increased in contaminated soils cultivated with miscanthus, compared to unplanted soils. These results verify the capacity of miscanthus to restore polluted soil functionalities, indicating the positive effect of phytostabilization on soil biological activities. In conclusion, revegetating polluted soils with miscanthus plants can restore their capacity to perform biochemically and biologically. |
Auteurs : Détriché Sébastien; Douay Francis Distribution spatiale et enrichissements en ETM dans les sols agricoles du Nord-Pas-de-Calais @book{Detriche2017, title = {Distribution spatiale et enrichissements en ETM dans les sols agricoles du Nord-Pas-de-Calais}, author = {Sébastien Détriché and Francis Douay}, year = {2017}, date = {2017-01-01}, booktitle = {Les cartes et les données pédologiques, des outils au service des territoires}, volume = {pp70-73, Educagri Editions}, note = {OV}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {book} } |
Auteurs : Détriché Sébastien; Douay Francis Evaluation et conséquences de la contamination des sols en ETM autour d’une ancienne fonderie de plomb et de zinc Educagri Editions, 74-77, @book{Detriche2017a, title = {Evaluation et conséquences de la contamination des sols en ETM autour d’une ancienne fonderie de plomb et de zinc}, author = {Sébastien Détriché and Francis Douay}, year = {2017}, date = {2017-01-01}, booktitle = {Les cartes et les données pédologiques, des outils au service des territoires}, publisher = {Educagri Editions, 74-77}, note = {OV}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {book} } |
Auteurs : Détriché Sébastien; Douay Francis Interpréter la présence d’élémnets traces métalliques @book{Detriche2017b, title = {Interpréter la présence d’élémnets traces métalliques}, author = {Sébastien Détriché and Francis Douay}, year = {2017}, date = {2017-01-01}, booktitle = {Les cartes et les données pédologiques, des outils au service des territoires}, volume = {pp64-65, Educagri Editions}, note = {OV}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {book} } |
Auteurs : Douay Francis; Lefebvre Gaëtan; Branchu Philippe; Pelfrêne Aurélie; Lebeau Thierry; Rémy Elisabeth Jardins collectifs urbains et contaminations des sols : retours d’expérience sur l’évaluation des risques sanitaires International congress - Sustainable Urban Agricultures: Vector for the Ecological Transition, 6-9 juin 2017, Toulouse @conference{Douay2017, title = {Jardins collectifs urbains et contaminations des sols : retours d’expérience sur l’évaluation des risques sanitaires}, author = {Francis Douay and Gaëtan Lefebvre and Philippe Branchu and Aurélie Pelfrêne and Thierry Lebeau and Elisabeth Rémy}, year = {2017}, date = {2017-01-01}, booktitle = {International congress - Sustainable Urban Agricultures: Vector for the Ecological Transition, 6-9 juin 2017, Toulouse}, abstract = {Avec la montée en puissance de préoccupations en lien avec l’environnement et le « manger sain », on constate une demande croissante pour les jardins collectifs urbains (jardins familiaux, partagés, pédagogiques ou encore d’insertion) (canavese, 2014). cependant, résultant de processus géologiques et pédologiques hétérogènes (pouvant dans certains cas être à l’origine d’anomalies avec fortes concentrations en certains éléments métalliques, cf. jean-soro et al. 2014; bouquet et al., 2016), leurs sols sont des milieux très complexes et réactifs aux activités humaines et industrielles (douay, 2008). les jardins collectifs présentent la particularité d’être très souvent en milieu urbain ou périurbain et leurs sols ont été fréquemment remaniés, d’où la difficulté de retracer l’historique et, parfois, l’origine des contaminants. de fait, les jardins sont souvent aménagés sur des espaces qui ont été exposés par le passé à une, voire plusieurs sources de contamination. à ce jour, demeurent de nombreuses sources de contaminations (potentielles ou avérées) telles que la proximité d’industries, d’axes routiers ou ferroviaires susceptibles d’impacter la qualité de ces milieux comme supports de cultures, l'utilisation fréquente de produits phytosanitaires (même lorsque la charte du jardin ou de la ville l’interdit) qui peuvent conduire à modifier le fonctionnement du sol, ou encore l’utilisation pour l’irrigation d’eau contaminée. à cela s’ajoutent les sources de contaminations urbaines issues de la circulation automobile, du chauffage et de l’altération des constructions et des matériaux. dans ce contexte, les jardins peuvent présenter un risque du fait d’une contamination de leurs sols et plus globalement du milieu où ils sont localisés, surtout lorsqu’ils concernent une population dite « sensible » (enfants, femmes enceintes, personnes âgées). l’exposition aux polluants peut être liée à la consommation de légumes et/ou à l’inhalation ou à l’ingestion de particules de terre contaminées. une autre voie d’exposition est celle de la pollution atmosphérique dont les effets sont accentués par l’activité physique associée au jardinage. d’un point de vue réglementaire et contrairement à l’air ou à l’eau, le sol a un statut très particulier puisqu’il n’est pas protégé en tant que tel. l’absence de valeurs seuils réglementant les usages des sols pèse sur la gestion des jardins urbains, espaces récréatifs et de productions alimentaires. en cas de contamination environnementale avérée, la démarche nationale repose sur les procédures réglementaires d’évaluation de risques sanitaires des sites et sols pollués. dans le cadre d’une démarche pluridisciplinaire, il a été décidé de centrer notre attention sur l’outil « evaluation quantitative des risques sanitaires –eqrs » préconisé dans le cadre de la méthodologie nationale et appliqué à la problématique des jardins associatifs. après un rappel des étapes relatives à cet outil, seront exposés les contextes et les démarches de cinq études réalisées sur des jardins familiaux français situés dans différents contextes environnementaux. le retour d’expérience sur l’analyse des eqrs étudiées a mis en évidence six points majeurs, lesquels seront présentés et discutés : - les compétences du maître d’ouvrage / maître d’oeuvre pour définir ce qui est à faire ou à ne pas faire dans le cadre d’une eqrs ; - les données nécessaires pour la réalisation d’une eqrs en soulignant l’importance du diagnostic historique ; - les incertitudes liées à la mesure des concentrations en polluants dans les matrices échantillonnées ; - les difficultés qui résultent de l’absence de valeurs réglementaires pour déclarer qu’un sol est contaminé et du recours à des référentiels plus ou moins adaptés au contexte et aux objectifs de la démarche ; - les incertitudes sur les paramètres à considérer dans l’eqrs (quantités de particules de terre et de légumes ingérés, part des légumes autoproduits…) ; - la perception du sol par les jardiniers et la nécessité de définir un langage commun pour ne pas générer de problèmes d’interprétation et répondre aux questions que se posent ces acteurs. l’analyse des cinq exemples d’eqrs atteste l’importance des paramètres à considérer pour une telle démarche. le présent travail représente un apport méthodologique en vue d’essayer de guider les pratiques et aussi, de répondre aux collectivités, lesquelles sont de plus en plus confrontées à des problèmes de contamination des sols de jardin et veulent s’engager dans la réalisation d’une eqrs. au vu de certains résultats, des jardiniers vont se documenter, chercher des réponses auprès des mairies, des bureaux d’études, de l’ars, de scientifiques… l’expérience souligne aussi l’absence de référent qui pourrait renseigner sur la qualité des sols et de leur gestion en milieu contaminé. la restitution des résultats auprès des gestionnaires de jardins et des populations concernées nécessite une réflexion approfondie associant les parties concernées et des spécialistes de la santé.}, note = {ACTI}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } Avec la montée en puissance de préoccupations en lien avec l’environnement et le « manger sain », on constate une demande croissante pour les jardins collectifs urbains (jardins familiaux, partagés, pédagogiques ou encore d’insertion) (canavese, 2014). cependant, résultant de processus géologiques et pédologiques hétérogènes (pouvant dans certains cas être à l’origine d’anomalies avec fortes concentrations en certains éléments métalliques, cf. jean-soro et al. 2014; bouquet et al., 2016), leurs sols sont des milieux très complexes et réactifs aux activités humaines et industrielles (douay, 2008). les jardins collectifs présentent la particularité d’être très souvent en milieu urbain ou périurbain et leurs sols ont été fréquemment remaniés, d’où la difficulté de retracer l’historique et, parfois, l’origine des contaminants. de fait, les jardins sont souvent aménagés sur des espaces qui ont été exposés par le passé à une, voire plusieurs sources de contamination. à ce jour, demeurent de nombreuses sources de contaminations (potentielles ou avérées) telles que la proximité d’industries, d’axes routiers ou ferroviaires susceptibles d’impacter la qualité de ces milieux comme supports de cultures, l'utilisation fréquente de produits phytosanitaires (même lorsque la charte du jardin ou de la ville l’interdit) qui peuvent conduire à modifier le fonctionnement du sol, ou encore l’utilisation pour l’irrigation d’eau contaminée. à cela s’ajoutent les sources de contaminations urbaines issues de la circulation automobile, du chauffage et de l’altération des constructions et des matériaux. dans ce contexte, les jardins peuvent présenter un risque du fait d’une contamination de leurs sols et plus globalement du milieu où ils sont localisés, surtout lorsqu’ils concernent une population dite « sensible » (enfants, femmes enceintes, personnes âgées). l’exposition aux polluants peut être liée à la consommation de légumes et/ou à l’inhalation ou à l’ingestion de particules de terre contaminées. une autre voie d’exposition est celle de la pollution atmosphérique dont les effets sont accentués par l’activité physique associée au jardinage. d’un point de vue réglementaire et contrairement à l’air ou à l’eau, le sol a un statut très particulier puisqu’il n’est pas protégé en tant que tel. l’absence de valeurs seuils réglementant les usages des sols pèse sur la gestion des jardins urbains, espaces récréatifs et de productions alimentaires. en cas de contamination environnementale avérée, la démarche nationale repose sur les procédures réglementaires d’évaluation de risques sanitaires des sites et sols pollués. dans le cadre d’une démarche pluridisciplinaire, il a été décidé de centrer notre attention sur l’outil « evaluation quantitative des risques sanitaires –eqrs » préconisé dans le cadre de la méthodologie nationale et appliqué à la problématique des jardins associatifs. après un rappel des étapes relatives à cet outil, seront exposés les contextes et les démarches de cinq études réalisées sur des jardins familiaux français situés dans différents contextes environnementaux. le retour d’expérience sur l’analyse des eqrs étudiées a mis en évidence six points majeurs, lesquels seront présentés et discutés : - les compétences du maître d’ouvrage / maître d’oeuvre pour définir ce qui est à faire ou à ne pas faire dans le cadre d’une eqrs ; - les données nécessaires pour la réalisation d’une eqrs en soulignant l’importance du diagnostic historique ; - les incertitudes liées à la mesure des concentrations en polluants dans les matrices échantillonnées ; - les difficultés qui résultent de l’absence de valeurs réglementaires pour déclarer qu’un sol est contaminé et du recours à des référentiels plus ou moins adaptés au contexte et aux objectifs de la démarche ; - les incertitudes sur les paramètres à considérer dans l’eqrs (quantités de particules de terre et de légumes ingérés, part des légumes autoproduits…) ; - la perception du sol par les jardiniers et la nécessité de définir un langage commun pour ne pas générer de problèmes d’interprétation et répondre aux questions que se posent ces acteurs. l’analyse des cinq exemples d’eqrs atteste l’importance des paramètres à considérer pour une telle démarche. le présent travail représente un apport méthodologique en vue d’essayer de guider les pratiques et aussi, de répondre aux collectivités, lesquelles sont de plus en plus confrontées à des problèmes de contamination des sols de jardin et veulent s’engager dans la réalisation d’une eqrs. au vu de certains résultats, des jardiniers vont se documenter, chercher des réponses auprès des mairies, des bureaux d’études, de l’ars, de scientifiques… l’expérience souligne aussi l’absence de référent qui pourrait renseigner sur la qualité des sols et de leur gestion en milieu contaminé. la restitution des résultats auprès des gestionnaires de jardins et des populations concernées nécessite une réflexion approfondie associant les parties concernées et des spécialistes de la santé. |
Auteurs : Ghemari Chedliya; Waterlot Christophe; Ayari Anas; Leclercq Julie; Douay Francis; Nasri-Ammar Karima Assessment of heavy metals in soil and terrestrial isopod Porcellio laevis in Tunisian industrialized areas Environmental Earth Sciences, 76, pp. 623-6361 @article{Ghemari2017, title = {Assessment of heavy metals in soil and terrestrial isopod Porcellio laevis in Tunisian industrialized areas}, author = {Chedliya Ghemari and Christophe Waterlot and Anas Ayari and Julie Leclercq and Francis Douay and Karima Nasri-Ammar}, year = {2017}, date = {2017-01-01}, journal = {Environmental Earth Sciences}, volume = {76}, pages = {623-636}, abstract = {In this study, data on several metals (cd, pb, zn and cu) in soil and isopod porcellio laevis taken at 21 sites from the most important industrial areas in tunisia (bizerte, nabeul, zaghouan, sfax and gabes) were presented. heavy metal concentrations in both soil samples and isopods were determined using atomic absorption spectrometry. soil contamination was estimated using the contamination factor (cf). on the other hand, the bioaccumulation factor (baf) was determined to estimate metal accumulation in isopods. the cf values show that the level of contamination varies between sampled soils, which may be due to the source of pollution at each site. the baf values allow defining the order of accumulation in p. laevis which was classified for the majority of the sampled sites as a macro-concentrator of cu and zn and a deconcentrator of cd with some exceptions. a principal component analysis (pca) was conducted between soil properties (ph, om and caco3) and metal concentrations in soils. through pca, we obtained four groups in which soils were distinguished by their physicochemical properties and their metal concentrations. moreover, linear multiple regressions with a downward stepwise procedure were conducted to test the relationships between the physicochemical parameters and metal concentrations in both soils and isopods. thus, positive correlations (0.78 < r 2 < 0.99) were obtained for pb considering dataset from the groups 1, 2 and for zn with data of groups 2 and 3. finally, results showed that p. laevis could be used as a bio-indicator for monitoring and reducing the impact of pollution in terrestrial ecosystems}, note = {ACL}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {article} } In this study, data on several metals (cd, pb, zn and cu) in soil and isopod porcellio laevis taken at 21 sites from the most important industrial areas in tunisia (bizerte, nabeul, zaghouan, sfax and gabes) were presented. heavy metal concentrations in both soil samples and isopods were determined using atomic absorption spectrometry. soil contamination was estimated using the contamination factor (cf). on the other hand, the bioaccumulation factor (baf) was determined to estimate metal accumulation in isopods. the cf values show that the level of contamination varies between sampled soils, which may be due to the source of pollution at each site. the baf values allow defining the order of accumulation in p. laevis which was classified for the majority of the sampled sites as a macro-concentrator of cu and zn and a deconcentrator of cd with some exceptions. a principal component analysis (pca) was conducted between soil properties (ph, om and caco3) and metal concentrations in soils. through pca, we obtained four groups in which soils were distinguished by their physicochemical properties and their metal concentrations. moreover, linear multiple regressions with a downward stepwise procedure were conducted to test the relationships between the physicochemical parameters and metal concentrations in both soils and isopods. thus, positive correlations (0.78 < r 2 < 0.99) were obtained for pb considering dataset from the groups 1, 2 and for zn with data of groups 2 and 3. finally, results showed that p. laevis could be used as a bio-indicator for monitoring and reducing the impact of pollution in terrestrial ecosystems |
Auteurs : Janus Adeline; Goulas Anaïs; Pelfrêne Aurélie; Douay Francis; Waterlot Christophe Determination of PAHs by ultra fast liquid chromatography using a coreshell technology – Application to their determination after using biochar as adsorbent Measurement, 106, pp. 137-1421 @article{Janus2017a, title = {Determination of PAHs by ultra fast liquid chromatography using a coreshell technology – Application to their determination after using biochar as adsorbent}, author = {Adeline Janus and Anaïs Goulas and Aurélie Pelfrêne and Francis Douay and Christophe Waterlot}, year = {2017}, date = {2017-01-01}, journal = {Measurement}, volume = {106}, pages = {137-142}, abstract = {The c18 column (250 mm 4.6 mm) packed with 5 lm core-shell particles was used in an effort to optimize the separation of 8 polycyclic aromatic hydrocarbons under very low pressure values (44–52 bars), low temperature (16 c) and in 13 min. chromatogram and peaks of each polycyclic aromatic hydrocarbon studied, obtained using an ultra fast liquid chromatography fitted with this column and equipped with a diode array detector, were compared with those obtained using two other traditional c18 columns, packed with fully porous 5 lm particles, usually used in our laboratory and specifically designed for the separation of polycyclic aromatic hydrocarbons. thinnest peaks, highest sensitivity and efficiency are the main results related to the core-shell technology of the recent kinetex column compared to more common c18 columns due to the bed uniformity and packing quality of the recent core-shell column. on the other hand, reduced retention time was highlighted for each polycyclic aromatic hydrocarbon using the column packed with core-shell particles resulting from the lower surface area of these particles compared to the totally porous particles of the both other columns used. limits of detection and quantification, linearity range and repeatability were determined for the method developed with the core-shell column. a practical application was realized by evaluating the sorption capacity of the 8 polycyclic aromatic hydrocarbons studied in mixture using a biochar made from woody plants. to this end, the biochar was mixed with aqueous solution spiked with the mixture of polycyclic aromatic hydrocarbons and a kinetic study of their sorption was conducted. sorption percentage was higher than 89% for each polycyclic aromatic hydrocarbon studied showing that the studied biochar could be good and low cost alternative products for the sorption of this type of pollutants.}, note = {ACL}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {article} } The c18 column (250 mm 4.6 mm) packed with 5 lm core-shell particles was used in an effort to optimize the separation of 8 polycyclic aromatic hydrocarbons under very low pressure values (44–52 bars), low temperature (16 c) and in 13 min. chromatogram and peaks of each polycyclic aromatic hydrocarbon studied, obtained using an ultra fast liquid chromatography fitted with this column and equipped with a diode array detector, were compared with those obtained using two other traditional c18 columns, packed with fully porous 5 lm particles, usually used in our laboratory and specifically designed for the separation of polycyclic aromatic hydrocarbons. thinnest peaks, highest sensitivity and efficiency are the main results related to the core-shell technology of the recent kinetex column compared to more common c18 columns due to the bed uniformity and packing quality of the recent core-shell column. on the other hand, reduced retention time was highlighted for each polycyclic aromatic hydrocarbon using the column packed with core-shell particles resulting from the lower surface area of these particles compared to the totally porous particles of the both other columns used. limits of detection and quantification, linearity range and repeatability were determined for the method developed with the core-shell column. a practical application was realized by evaluating the sorption capacity of the 8 polycyclic aromatic hydrocarbons studied in mixture using a biochar made from woody plants. to this end, the biochar was mixed with aqueous solution spiked with the mixture of polycyclic aromatic hydrocarbons and a kinetic study of their sorption was conducted. sorption percentage was higher than 89% for each polycyclic aromatic hydrocarbon studied showing that the studied biochar could be good and low cost alternative products for the sorption of this type of pollutants. |
Auteurs : Janus Adeline; Pelfrêne Aurélie; Sahmer Karin; Heymans Sophie; Deboffe Christophe; Douay Francis; Waterlot Christophe Value of biochars from Miscanthus x giganteus cultivated on contaminated soils to decrease the availability of metals in multicontaminated aqueous solutions Environmental Science and Pollution Research, 24(22), pp. 18204-182171 @article{Janus2017b, title = {Value of biochars from Miscanthus x giganteus cultivated on contaminated soils to decrease the availability of metals in multicontaminated aqueous solutions}, author = {Adeline Janus and Aurélie Pelfrêne and Karin Sahmer and Sophie Heymans and Christophe Deboffe and Francis Douay and Christophe Waterlot}, year = {2017}, date = {2017-01-01}, journal = {Environmental Science and Pollution Research}, volume = {24}, number = {22}, pages = {18204-18217}, abstract = {The objective of this study was to evaluate the sorption efficiency of eight biochars, made from miscanthus x giganteus cultivated on contaminated agricultural soil, in aqueous solutions contaminated with metals alone or mixed with polycyclic aromatic hydrocarbons. these biochars were produced in different pyrolysis conditions (temperature, 400/600 °c; heating rate, 5/10 °c min−1; duration, 45/90 min) and compared with an uncontaminated commercialized biochar made of wood. the physicochemical characterization of the miscanthus biochars confirmed the impact of the pyrolysis on the biochar parameters with substantial differences between the biochars in terms of ph, cation exchange capacity, and specific surface area. the sorption experiment showed higher sorption efficiency of cd, pb, and zn for the miscanthus biochars produced at 600 °c compared with the biochars produced at 400 °c when the aqueous solutions were mono- or multicontaminated. furthermore, the desorption study showed that the sorption process was largely irreversible. therefore, the high sorption capacity of miscanthus biochars and the low sorption reversibility confirmed that these biochars are a suitable sorbent for metals.}, note = {ACL}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {article} } The objective of this study was to evaluate the sorption efficiency of eight biochars, made from miscanthus x giganteus cultivated on contaminated agricultural soil, in aqueous solutions contaminated with metals alone or mixed with polycyclic aromatic hydrocarbons. these biochars were produced in different pyrolysis conditions (temperature, 400/600 °c; heating rate, 5/10 °c min−1; duration, 45/90 min) and compared with an uncontaminated commercialized biochar made of wood. the physicochemical characterization of the miscanthus biochars confirmed the impact of the pyrolysis on the biochar parameters with substantial differences between the biochars in terms of ph, cation exchange capacity, and specific surface area. the sorption experiment showed higher sorption efficiency of cd, pb, and zn for the miscanthus biochars produced at 600 °c compared with the biochars produced at 400 °c when the aqueous solutions were mono- or multicontaminated. furthermore, the desorption study showed that the sorption process was largely irreversible. therefore, the high sorption capacity of miscanthus biochars and the low sorption reversibility confirmed that these biochars are a suitable sorbent for metals. |
Auteurs : Labidi Sonia; Firmin Stéphane; Verdin Anthony; Bidar Géraldine; Laruelle Frédéric; Douay Francis; Shirali Pirouz; Fontaine Joël; Lounès-Hadj-Sahraoui Anissa Nature of fly ash amendments differently influences oxidative stress alleviation in four forest tree species and metal trace element phytostabilization in aged contaminated soil: A long-term field experiment Ecotoxicology and Environmental Safety, 138, pp. 190-1981 @article{Labidi2017, title = {Nature of fly ash amendments differently influences oxidative stress alleviation in four forest tree species and metal trace element phytostabilization in aged contaminated soil: A long-term field experiment}, author = {Sonia Labidi and Stéphane Firmin and Anthony Verdin and Géraldine Bidar and Frédéric Laruelle and Francis Douay and Pirouz Shirali and Joël Fontaine and Anissa Lounès-Hadj-Sahraoui}, year = {2017}, date = {2017-01-01}, journal = {Ecotoxicology and Environmental Safety}, volume = {138}, pages = {190-198}, abstract = {Aided phytostabilization using coal fly ashes (cfas) is an interesting technique to clean-up polluted soils and valorizing industrial wastes. in this context, our work aims to study the effect of two cfas: silico-aluminous (cfa1) and sulfo-calcic (cfa2) ones, 10 years after their addition, on the phytostabilization of a highly cd (cadmium), pb (lead) and zn (zinc) contaminated agricultural soil, with four forest tree species: robinia pseudoacacia, alms glutinosa, acer pseudoplatanus and salix alba. to assess the effect of cfas on trees, leaf fatty acid composition, malondialdehyde (mda), oxidized and reduced glutathione contents ratio (gssg: gsh), 8hydroxy-2'-deoxyguanosine (8-ohdg), peroxidase (po) and superoxide dismutase (sod) activities were examined. our results showed that cfa amendments decreased the cac1(2-)extractable fraction of cd and zn from the soil. however, no significant effect was observed on metal trace element (mte) concentrations in leaves. fatty acid percentages were only affected by the addition of sulfo-calcic cfa. the most affected species were a. glutinosa and r. pseudoacacia in which c16:0, c18:0 and c18:2 percentages increased significantly whereas the c18:3 decreased. the addition of sulfo-calcic cfa induced the antioxidant systems response in tree leaves. an increase of sod and pod activities in leaves of trees planted on the cfa2-amended plot was recorded. conversely, silico-aluminous cfa generated a reduction of lipid and dna oxidation associated with the absence or low induction of anti-oxidative processes. our study evidenced oxidative stress alleviation in tree leaves due to cfa amendments. mte mobility in contaminated soil and their accumulation in leaves differed with the nature of cfa amendments and the selected tree species.}, note = {ACL}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {article} } Aided phytostabilization using coal fly ashes (cfas) is an interesting technique to clean-up polluted soils and valorizing industrial wastes. in this context, our work aims to study the effect of two cfas: silico-aluminous (cfa1) and sulfo-calcic (cfa2) ones, 10 years after their addition, on the phytostabilization of a highly cd (cadmium), pb (lead) and zn (zinc) contaminated agricultural soil, with four forest tree species: robinia pseudoacacia, alms glutinosa, acer pseudoplatanus and salix alba. to assess the effect of cfas on trees, leaf fatty acid composition, malondialdehyde (mda), oxidized and reduced glutathione contents ratio (gssg: gsh), 8hydroxy-2'-deoxyguanosine (8-ohdg), peroxidase (po) and superoxide dismutase (sod) activities were examined. our results showed that cfa amendments decreased the cac1(2-)extractable fraction of cd and zn from the soil. however, no significant effect was observed on metal trace element (mte) concentrations in leaves. fatty acid percentages were only affected by the addition of sulfo-calcic cfa. the most affected species were a. glutinosa and r. pseudoacacia in which c16:0, c18:0 and c18:2 percentages increased significantly whereas the c18:3 decreased. the addition of sulfo-calcic cfa induced the antioxidant systems response in tree leaves. an increase of sod and pod activities in leaves of trees planted on the cfa2-amended plot was recorded. conversely, silico-aluminous cfa generated a reduction of lipid and dna oxidation associated with the absence or low induction of anti-oxidative processes. our study evidenced oxidative stress alleviation in tree leaves due to cfa amendments. mte mobility in contaminated soil and their accumulation in leaves differed with the nature of cfa amendments and the selected tree species. |
Auteurs : Leclercq-Dransart Julie; Santorufo Lucia; Pernin Céline; Louvel Brice; Demuynck Sylvain; Grumiaux Fabien; Douay Francis; Leprêtre Alain Efficacité de méthodes de phytomanagement sur la fonctionnalité de sols fortement contaminés par les métaux Société Française d'Ecotoxicologie Fondamentale et Appliquée, 29-30 juin 2017, Lille @conference{Leclercq-Dransart2017c, title = {Efficacité de méthodes de phytomanagement sur la fonctionnalité de sols fortement contaminés par les métaux}, author = {Julie Leclercq-Dransart and Lucia Santorufo and Céline Pernin and Brice Louvel and Sylvain Demuynck and Fabien Grumiaux and Francis Douay and Alain Leprêtre}, year = {2017}, date = {2017-01-01}, booktitle = {Société Française d'Ecotoxicologie Fondamentale et Appliquée, 29-30 juin 2017, Lille}, abstract = {Le site atelier metaleurop situé dans l'ancien bassin minier du nord - pas de calais, présente des paysages fortement dégradés par les activités humaines (urbanisation, activités industrielles, agriculture intensive). la forte contamination des sols en métaux aux alentours de l’ancienne fonderie metaleurop nord est bien connue et plusieurs études ont été menées sur le secteur (e.g: comportement des métaux dans le sol et phytomanagement [1,2], risques sanitaires [3] et communautés biologiques [4,5]). une expérimentation a été mise en place sur le terrain pendant 10 mois avec pour objectif d'évaluer l'efficacité de méthodes de remédiation mises en place depuis 17 ans sur des sols contaminés en métaux et fortement dégradés. il s'agit de plantations mixtes ou mono-spécifiques couplées ou non à l'utilisation de cendres volantes comme amendement pour limiter la mobilité des métaux dans le sol. l'approche mise en oeuvre est basée sur le suivi de la dégradation d'une litière de peuplier selon les différents modes de gestion et sa colonisation par les organismes de la mésofaune, connus pour intervenir lors de la dégradation de la matière organique et fournir de nombreux services écosystémiques [6]. la technique des sacs de litière a été utilisée. les principaux résultats montrent que la dégradation de la litière est significativement plus rapide sur la plantation mixte que la monospécifique en été ce qui pourrait s'expliquer par une plus forte abondance de la mésofaune et/ou des conditions abiotiques (humidité, ombrage…) plus favorables à l'activité de celle-ci. en revanche, aucune différence significative n'a été observée entre les parcelles amendées (dégradation de la litière apportée et communautés mésofauniques) et celle soumise au seul phytomanagement. on note que les communautés sont perturbées et que l'abondance des organismes détritivores est faible ce qui pourrait expliquer une dégradation des litières plus lente qu'attendue. ainsi, parmi les méthodes de phytomanagement testées sur le site expérimental, les plantations mixtes pourraient apporter un bénéfice pour la restauration des sols dégradés. l'emploi de cendres volantes n'a pas d'incidence notable sur la fonctionnalité des sols.}, note = {ACTN}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } Le site atelier metaleurop situé dans l'ancien bassin minier du nord - pas de calais, présente des paysages fortement dégradés par les activités humaines (urbanisation, activités industrielles, agriculture intensive). la forte contamination des sols en métaux aux alentours de l’ancienne fonderie metaleurop nord est bien connue et plusieurs études ont été menées sur le secteur (e.g: comportement des métaux dans le sol et phytomanagement [1,2], risques sanitaires [3] et communautés biologiques [4,5]). une expérimentation a été mise en place sur le terrain pendant 10 mois avec pour objectif d'évaluer l'efficacité de méthodes de remédiation mises en place depuis 17 ans sur des sols contaminés en métaux et fortement dégradés. il s'agit de plantations mixtes ou mono-spécifiques couplées ou non à l'utilisation de cendres volantes comme amendement pour limiter la mobilité des métaux dans le sol. l'approche mise en oeuvre est basée sur le suivi de la dégradation d'une litière de peuplier selon les différents modes de gestion et sa colonisation par les organismes de la mésofaune, connus pour intervenir lors de la dégradation de la matière organique et fournir de nombreux services écosystémiques [6]. la technique des sacs de litière a été utilisée. les principaux résultats montrent que la dégradation de la litière est significativement plus rapide sur la plantation mixte que la monospécifique en été ce qui pourrait s'expliquer par une plus forte abondance de la mésofaune et/ou des conditions abiotiques (humidité, ombrage…) plus favorables à l'activité de celle-ci. en revanche, aucune différence significative n'a été observée entre les parcelles amendées (dégradation de la litière apportée et communautés mésofauniques) et celle soumise au seul phytomanagement. on note que les communautés sont perturbées et que l'abondance des organismes détritivores est faible ce qui pourrait expliquer une dégradation des litières plus lente qu'attendue. ainsi, parmi les méthodes de phytomanagement testées sur le site expérimental, les plantations mixtes pourraient apporter un bénéfice pour la restauration des sols dégradés. l'emploi de cendres volantes n'a pas d'incidence notable sur la fonctionnalité des sols. |
Auteurs : Pelfrêne Aurélie; Cave Mark-R.; Wragg Joanna; Douay Francis In vitro investigations of human bioaccessibility from reference materials using simulated lung fluids International Journal of Environmental Research and Public Health, 14(2), pp. 1121 @article{Pelfrene2017b, title = {In vitro investigations of human bioaccessibility from reference materials using simulated lung fluids}, author = {Aurélie Pelfrêne and Mark-R. Cave and Joanna Wragg and Francis Douay}, year = {2017}, date = {2017-01-01}, journal = {International Journal of Environmental Research and Public Health}, volume = {14}, number = {2}, pages = {112}, abstract = {An investigation for assessing pulmonary bioaccessibility of metals from reference materials is presented using simulated lung fluids. the objective of this paper was to contribute to an enhanced understanding of airborne particulate matter and its toxic potential following inhalation. a large set of metallic elements (ba, cd, co, cr, cu, mn, ni, pb, sr, and zn) was investigated using three lung fluids (phosphate-buffered saline, gamble’s solution and artificial lysosomal fluid) on three standard reference materials representing different types of particle sources. composition of the leaching solution and four solid-to-liquid (s/l) ratios were tested. the results showed that bioaccessibility was speciation- (i.e., distribution) and element-dependent, with percentages varying from 0.04% for pb to 86.0% for cd. the higher extraction of metallic elements was obtained with the artificial lysosomal fluid, in which a relative stability of bioaccessibility was observed in a large range of s/l ratios from 1/1000 to 1/10,000. for further investigations, it is suggested that this method be used to assess lung bioaccessibility of metals from smelter-impacted dusts.}, note = {ACL}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {article} } An investigation for assessing pulmonary bioaccessibility of metals from reference materials is presented using simulated lung fluids. the objective of this paper was to contribute to an enhanced understanding of airborne particulate matter and its toxic potential following inhalation. a large set of metallic elements (ba, cd, co, cr, cu, mn, ni, pb, sr, and zn) was investigated using three lung fluids (phosphate-buffered saline, gamble’s solution and artificial lysosomal fluid) on three standard reference materials representing different types of particle sources. composition of the leaching solution and four solid-to-liquid (s/l) ratios were tested. the results showed that bioaccessibility was speciation- (i.e., distribution) and element-dependent, with percentages varying from 0.04% for pb to 86.0% for cd. the higher extraction of metallic elements was obtained with the artificial lysosomal fluid, in which a relative stability of bioaccessibility was observed in a large range of s/l ratios from 1/1000 to 1/10,000. for further investigations, it is suggested that this method be used to assess lung bioaccessibility of metals from smelter-impacted dusts. |
Auteurs : Pelfrêne Aurélie; Sahmer Karin; Grard Olivier; Heyman Christophe; Douay Francis De l’acquisition de données environnementales et sociales à l’évaluation de l’exposition des jardiniers: Un retour d’expérience dans un contexte urbain fortement contaminé par les métaux International congress - Sustainable Urban Agricultures: Vector for the Ecological Transition, 6-9 juin 2017, Toulouse @conference{Pelfrene2017bb, title = {De l’acquisition de données environnementales et sociales à l’évaluation de l’exposition des jardiniers: Un retour d’expérience dans un contexte urbain fortement contaminé par les métaux}, author = {Aurélie Pelfrêne and Karin Sahmer and Olivier Grard and Christophe Heyman and Francis Douay}, year = {2017}, date = {2017-01-01}, booktitle = {International congress - Sustainable Urban Agricultures: Vector for the Ecological Transition, 6-9 juin 2017, Toulouse}, abstract = {Jusqu’à récemment, le jardinage avait souvent pour vocation principale de subvenir aux besoins alimentaires. de nos jours, les motivations des jardiniers sont beaucoup plus variées et il est admis que le jardin joue aussi un rôle social et de support de biodiversité. si le jardinage est effectué le plus souvent en milieu rural (près de 51% selon l’insee, 1993), il se pratique aussi de plus en plus souvent en milieu périurbain ou urbain. se pose alors la question de la qualité des sols et des productions cultivées au regard de pollutions potentielles résultant des activités humaines (chauffage urbain, circulation automobile, activités industrielles…). cette problématique, qui reste rarement étudiée, est d’autant plus importante dans les régions avec un lourd passé industriel comme dans l’ancien bassin minier du nord – pas de calais. depuis plus de 20 ans, des travaux y sont réalisés et ceci, plus spécifiquement dans un secteur compris entre lens et douai où deux fonderies de plomb et de zinc ont rejeté par le passé des quantités considérables de poussières métalliques. leurs retombées ont conduit à une contamination massive des sols notamment en plomb, cadmium et zinc. en partenariat avec les services sanitaires et pour évaluer les dangers, des investigations sont menées dans les jardins aux alentours de l’ancienne fonderie de plomb metaleurop nord (noyelles-godault) où plus de 900 potagers ont été repérés au moyen de photographies aériennes. sur les 530 jardiniers qui ont été sollicités pour participer à une étude visant à mieux connaître ces milieux et les dangers associés à leur contamination, 115 ont accepté. l’échantillonnage des sols et des productions a été réalisé durant l’année culturale 2013. les pratiques culturales et les habitudes alimentaires ont aussi été caractérisées au moyen d’enquêtes. les résultats ont confirmé le très fort degré de contamination de la plupart des sols des jardins étudiés, lequel ne peut être expliqué par les seules retombées de poussières. des apports de matériaux contaminés et diverses pratiques ont souvent conduit à accroître notablement les concentrations en polluants des sols. il a été dressé un bilan des productions selon leur aptitude à accumuler les métaux dans les organes consommés et préparés comme le ferait un consommateur soigneux (lavage, épluchage). sur les 1566 échantillons de légumes, aromates et fruits analysés, 51% présentent des concentrations en cadmium et plomb qui excèdent les valeurs réglementant la consommation des denrées alimentaires. au regard de ces résultats, s’est posée la question de l’exposition des jardiniers en lien avec la consommation de leurs productions potagères mais aussi avec l’ingestion de particules de sols. pour mieux évaluer cette exposition, la bioaccessibilité orale des métaux a été prise en compte au lieu des concentrations totales classiquement mesurées dans les légumes et les sols. les résultats obtenus ont été présentés aux jardiniers lors d’entretiens individuels. la restitution a été complétée par des préconisations culturales et/ou alimentaires personnalisées visant à réduire l’imprégnation des populations au plomb et au cadmium. l’évaluation des effets de ces restitutions sur les pratiques a été réalisée un an après la fin de la restitution sur la base d’un nouvel entretien avec les jardiniers concernés. sera présenté un retour d’expérience sur cette démarche, de l’échantillonnage des sols et des productions jusqu’à la restitution auprès des jardiniers des données acquises.}, note = {AFF}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } Jusqu’à récemment, le jardinage avait souvent pour vocation principale de subvenir aux besoins alimentaires. de nos jours, les motivations des jardiniers sont beaucoup plus variées et il est admis que le jardin joue aussi un rôle social et de support de biodiversité. si le jardinage est effectué le plus souvent en milieu rural (près de 51% selon l’insee, 1993), il se pratique aussi de plus en plus souvent en milieu périurbain ou urbain. se pose alors la question de la qualité des sols et des productions cultivées au regard de pollutions potentielles résultant des activités humaines (chauffage urbain, circulation automobile, activités industrielles…). cette problématique, qui reste rarement étudiée, est d’autant plus importante dans les régions avec un lourd passé industriel comme dans l’ancien bassin minier du nord – pas de calais. depuis plus de 20 ans, des travaux y sont réalisés et ceci, plus spécifiquement dans un secteur compris entre lens et douai où deux fonderies de plomb et de zinc ont rejeté par le passé des quantités considérables de poussières métalliques. leurs retombées ont conduit à une contamination massive des sols notamment en plomb, cadmium et zinc. en partenariat avec les services sanitaires et pour évaluer les dangers, des investigations sont menées dans les jardins aux alentours de l’ancienne fonderie de plomb metaleurop nord (noyelles-godault) où plus de 900 potagers ont été repérés au moyen de photographies aériennes. sur les 530 jardiniers qui ont été sollicités pour participer à une étude visant à mieux connaître ces milieux et les dangers associés à leur contamination, 115 ont accepté. l’échantillonnage des sols et des productions a été réalisé durant l’année culturale 2013. les pratiques culturales et les habitudes alimentaires ont aussi été caractérisées au moyen d’enquêtes. les résultats ont confirmé le très fort degré de contamination de la plupart des sols des jardins étudiés, lequel ne peut être expliqué par les seules retombées de poussières. des apports de matériaux contaminés et diverses pratiques ont souvent conduit à accroître notablement les concentrations en polluants des sols. il a été dressé un bilan des productions selon leur aptitude à accumuler les métaux dans les organes consommés et préparés comme le ferait un consommateur soigneux (lavage, épluchage). sur les 1566 échantillons de légumes, aromates et fruits analysés, 51% présentent des concentrations en cadmium et plomb qui excèdent les valeurs réglementant la consommation des denrées alimentaires. au regard de ces résultats, s’est posée la question de l’exposition des jardiniers en lien avec la consommation de leurs productions potagères mais aussi avec l’ingestion de particules de sols. pour mieux évaluer cette exposition, la bioaccessibilité orale des métaux a été prise en compte au lieu des concentrations totales classiquement mesurées dans les légumes et les sols. les résultats obtenus ont été présentés aux jardiniers lors d’entretiens individuels. la restitution a été complétée par des préconisations culturales et/ou alimentaires personnalisées visant à réduire l’imprégnation des populations au plomb et au cadmium. l’évaluation des effets de ces restitutions sur les pratiques a été réalisée un an après la fin de la restitution sur la base d’un nouvel entretien avec les jardiniers concernés. sera présenté un retour d’expérience sur cette démarche, de l’échantillonnage des sols et des productions jusqu’à la restitution auprès des jardiniers des données acquises. |
Auteurs : Pelfrêne Aurélie; Waterlot Christophe; Le-Bot Barbara; Glorennec Philippe; Douay Francis A decision-making tool for managing franch polluted sites: Toward an alternative approach to the Unified Barge Method 6-9 November 2017, Varsovie (Pologne) @conference{Pelfrene2017c, title = {A decision-making tool for managing franch polluted sites: Toward an alternative approach to the Unified Barge Method}, author = {Aurélie Pelfrêne and Christophe Waterlot and Barbara Le-Bot and Philippe Glorennec and Francis Douay}, year = {2017}, date = {2017-01-01}, booktitle = {6-9 November 2017, Varsovie (Pologne)}, note = {AFF}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } |
Auteurs : Pourrut Bertrand; Al-Souki Karim; Nsanganwimana Florien; Liné Clarisse; Bastia G; Douay Francis Use of Miscanthus x giganteus to phytomanage large contaminated areas: lessons from Northern France IV International conference “Plant Ontogenesis in Natural and Transformed Environment, 4-6 October 2017, Lviv (Ukraine) @conference{Pourrut2017, title = {Use of Miscanthus x giganteus to phytomanage large contaminated areas: lessons from Northern France}, author = {Bertrand Pourrut and Karim Al-Souki and Florien Nsanganwimana and Clarisse Liné and G Bastia and Francis Douay}, year = {2017}, date = {2017-01-01}, booktitle = {IV International conference “Plant Ontogenesis in Natural and Transformed Environment, 4-6 October 2017, Lviv (Ukraine)}, note = {COM}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } |
Auteurs : Pruvot Christelle; Douay Francis Usages et qualité des sols: Gestion des sols contaminés par les activités humaines Sols convoités, sols en danger: comment les protéger? Terre de liens - ISA Lille, 8 Juin 2017, Lille @conference{Pruvot2017, title = {Usages et qualité des sols: Gestion des sols contaminés par les activités humaines}, author = {Christelle Pruvot and Francis Douay}, year = {2017}, date = {2017-01-01}, booktitle = {Sols convoités, sols en danger: comment les protéger? Terre de liens - ISA Lille, 8 Juin 2017, Lille}, abstract = {Usages et qualité des sols: gestion des sols contaminés par les activités humaines}, note = {OV}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } Usages et qualité des sols: gestion des sols contaminés par les activités humaines |
Auteurs : Waterlot Christophe; Douay Francis; Pelfrêne Aurélie Chemical availability of Cd, Pb and Zn in anthropogenically polluted soil: Assessing the geochemical reactivity and oral bioaccessibility Pedosphere, 27(3), pp. 616-6291 @article{Waterlot2017b, title = {Chemical availability of Cd, Pb and Zn in anthropogenically polluted soil: Assessing the geochemical reactivity and oral bioaccessibility}, author = {Christophe Waterlot and Francis Douay and Aurélie Pelfrêne}, year = {2017}, date = {2017-01-01}, journal = {Pedosphere}, volume = {27}, number = {3}, pages = {616-629}, abstract = {The most recent in vitro tests used to determine metal bioaccessiblility are generally time-consuming and expensive. this study aimed at determining potential relationships between the concentrations of metals extracted using single-extraction methods and the concentrations of bioaccessible metals assessed by a harmonised in vitro test, the unified barge method (ubm). a total number of 27 soil samples were collected from kitchen gardens and lawns with various physicochemical parameters and contamination levels. significant relationships were obtained between cd, pb and zn extracted in gastric and gastrointestinal phases and using single extractions. the best relationhips were established using acetic and citric acids for cd, whereas for pb, citric acid and ethylenediaminetetraacetic acid (edta) were identified as the best extractants. these relationships were improved by means of a linear multiple regression with a downward stepwise procedure involving agronomic parameters (soil cation exchange capacity and assimilated p). this method highlighted the fact that the cation exchange capacity and p contents in soils were the two main parameters that controlled the human bioaccessibility of cd, pb and zn in the gastric phase. besides, the metal concentrations extracted with the acetic and citric acids correlated well with the metal concentrations in the gastric and gastrointestinal phases, suggesting that the bioaccessible metals were mainly in a soluble form, weakly bound to the organic matter and associated with the carbonates and the fe and mn oxides/hydroxides in soils.}, note = {ACL}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {article} } The most recent in vitro tests used to determine metal bioaccessiblility are generally time-consuming and expensive. this study aimed at determining potential relationships between the concentrations of metals extracted using single-extraction methods and the concentrations of bioaccessible metals assessed by a harmonised in vitro test, the unified barge method (ubm). a total number of 27 soil samples were collected from kitchen gardens and lawns with various physicochemical parameters and contamination levels. significant relationships were obtained between cd, pb and zn extracted in gastric and gastrointestinal phases and using single extractions. the best relationhips were established using acetic and citric acids for cd, whereas for pb, citric acid and ethylenediaminetetraacetic acid (edta) were identified as the best extractants. these relationships were improved by means of a linear multiple regression with a downward stepwise procedure involving agronomic parameters (soil cation exchange capacity and assimilated p). this method highlighted the fact that the cation exchange capacity and p contents in soils were the two main parameters that controlled the human bioaccessibility of cd, pb and zn in the gastric phase. besides, the metal concentrations extracted with the acetic and citric acids correlated well with the metal concentrations in the gastric and gastrointestinal phases, suggesting that the bioaccessible metals were mainly in a soluble form, weakly bound to the organic matter and associated with the carbonates and the fe and mn oxides/hydroxides in soils. |
Auteurs : Waterlot Christophe; Pruvot Christelle; Marot Franck; Douay Francis Impact of a phosphate amendment on the environmental availability and phytoavailability of Cd and Pb in moderately and highly carbonated kitchen garden soils Pedosphere, 27(3), pp. 588-6051 @article{Waterlot2017a, title = {Impact of a phosphate amendment on the environmental availability and phytoavailability of Cd and Pb in moderately and highly carbonated kitchen garden soils}, author = {Christophe Waterlot and Christelle Pruvot and Franck Marot and Francis Douay}, year = {2017}, date = {2017-01-01}, journal = {Pedosphere}, volume = {27}, number = {3}, pages = {588-605}, abstract = {The behaviour of metals mainly depends on soil ph, carbonate contents and contamination level, which should be considered for the management of contaminated soils. in this study, kitchen garden topsoils (0–25 cm) were sampled from the area around three smelters in france, with different cd and pb contamination levels. effect of a phosphate amendment (a mixture of diammonium phosphate and hydroxyapatite) on the environmental availability and phytoavailability of cd and pb was evaluated by different chemical extractions and cultivating lettuce (lactuca sativa l.), respectively. changes in the distribution of cd and pb were found in most contaminated soils after phosphate amendment. an increase of cd and pb in the residual phase was highlighted in almost all carbonated contaminated soils, whereas a decrease of pb in the exchangeable, water and acid- soluble phase was observed in most contaminated soils with the lowest carbonate contents. the concentrations of extractable cd and pb using calcium chloride and acetic and citric acids generally decreased after the soil amendment. lettuces grown on amended soils were acceptable for human consumption as regard to pb concentration. in contrast, some lettuces were unacceptable for human consumption, since the concentrations of cd in the leaves were higher than the european legislation limit. surprisingly, in carbonated soils with very low concentration of cd, the cd concentrations in lettuce reached up to the european legislation limit, making the lettuce unacceptable for human consumption. our study highlighted the fact that the total metal concentration in soils does not always allow to predict the metal accumulation in the edible parts of vegetables in order to make a judgement about their acceptability or unacceptability for human consumption. key words: chemical amendment, contamination, immobilisation, lettuce, metal, urban soil}, note = {ACL}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {article} } The behaviour of metals mainly depends on soil ph, carbonate contents and contamination level, which should be considered for the management of contaminated soils. in this study, kitchen garden topsoils (0–25 cm) were sampled from the area around three smelters in france, with different cd and pb contamination levels. effect of a phosphate amendment (a mixture of diammonium phosphate and hydroxyapatite) on the environmental availability and phytoavailability of cd and pb was evaluated by different chemical extractions and cultivating lettuce (lactuca sativa l.), respectively. changes in the distribution of cd and pb were found in most contaminated soils after phosphate amendment. an increase of cd and pb in the residual phase was highlighted in almost all carbonated contaminated soils, whereas a decrease of pb in the exchangeable, water and acid- soluble phase was observed in most contaminated soils with the lowest carbonate contents. the concentrations of extractable cd and pb using calcium chloride and acetic and citric acids generally decreased after the soil amendment. lettuces grown on amended soils were acceptable for human consumption as regard to pb concentration. in contrast, some lettuces were unacceptable for human consumption, since the concentrations of cd in the leaves were higher than the european legislation limit. surprisingly, in carbonated soils with very low concentration of cd, the cd concentrations in lettuce reached up to the european legislation limit, making the lettuce unacceptable for human consumption. our study highlighted the fact that the total metal concentration in soils does not always allow to predict the metal accumulation in the edible parts of vegetables in order to make a judgement about their acceptability or unacceptability for human consumption. key words: chemical amendment, contamination, immobilisation, lettuce, metal, urban soil |
Auteurs : Bayard Jean-Bernard; Douay Francis PHYTENER : Développement de la phytostabilisation à des fins énergétiques sur des sols contaminés par des métaux « Production, transformation et valorisation des biomasses : Une bioéconomie au service de la transition écologique et énergétique », Maison de la Chimie, 31 mars 2016, Paris @conference{Bayard2016, title = {PHYTENER : Développement de la phytostabilisation à des fins énergétiques sur des sols contaminés par des métaux}, author = {Jean-Bernard Bayard and Francis Douay}, year = {2016}, date = {2016-01-01}, booktitle = {« Production, transformation et valorisation des biomasses : Une bioéconomie au service de la transition écologique et énergétique », Maison de la Chimie, 31 mars 2016, Paris}, abstract = {Phytener : développement de la phytostabilisation à des fins énergétiques sur des sols contaminés par des métaux}, note = {COM}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } Phytener : développement de la phytostabilisation à des fins énergétiques sur des sols contaminés par des métaux |
Auteurs : Bidar Géraldine; Waterlot Christophe; Verdin Anthony; Proix Nicolas; Courcot Dominique; Détriché Sébastien; Fourrier Hervé; Richard Antoine; Douay Francis Sustainability of an in situ aided phytostabilisation on highly contaminated soils using fly ashes: Effects on the vertical distribution of physicochemical parameters and trace elements Journal of Environmental Management, 171, pp. 204-2161 @article{Bidar2016, title = {Sustainability of an in situ aided phytostabilisation on highly contaminated soils using fly ashes: Effects on the vertical distribution of physicochemical parameters and trace elements}, author = {Géraldine Bidar and Christophe Waterlot and Anthony Verdin and Nicolas Proix and Dominique Courcot and Sébastien Détriché and Hervé Fourrier and Antoine Richard and Francis Douay}, year = {2016}, date = {2016-01-01}, journal = {Journal of Environmental Management}, volume = {171}, pages = {204-216}, abstract = {Aided phytostabilisation using trees and fly ashes is a promising technique which has shown its effectiveness in the management of highly metal-contaminated soils. however, this success is generally established based on topsoil physicochemical analysis and short-term experiments. this paper focuses on the long-term effects of the afforestation and twofly ashes (silico-aluminous and sulfo-calcic called fa1 and fa2, respectively) by assessing the integrity of fly ashes 10 years after their incorporation into the soil as well as the vertical distribution of the physicochemical parameters and trace elements (tes) in the amended soils (f1 and f2) in comparison with a non-amended soil (r). ten years after the soil treatment, the particle size distribution analysis between fly ashes and their corresponding masses (fly ash þ soil particles) showed a loss or an agglomeration of finer particles. this evolution matches with the appearance of gypsum (caso4 2h2o) in fa2m instead of anhydrite (caso4), which is the major compound of fa2. this finding corresponds well with the dissolution and the lixiviation of ca, s and p included in fa2 along the f2 soil profile, generating an accumulation of these elements at 30 cm depth. however, no variation of te contaminationwas found between 0 and 25 cm depth in f2 soil except for cd. conversely, cd, pb, zn and hg enrichmentwas observed at 25 cm depth in the f1 soil, whereas no enrichmentwas observed for as. the fly ashes studied, and notably fa2, were able to reduce cd, pb and zn availability in soil and this capacity persists over the time despite their structural and chemical changes.}, note = {ACL}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {article} } Aided phytostabilisation using trees and fly ashes is a promising technique which has shown its effectiveness in the management of highly metal-contaminated soils. however, this success is generally established based on topsoil physicochemical analysis and short-term experiments. this paper focuses on the long-term effects of the afforestation and twofly ashes (silico-aluminous and sulfo-calcic called fa1 and fa2, respectively) by assessing the integrity of fly ashes 10 years after their incorporation into the soil as well as the vertical distribution of the physicochemical parameters and trace elements (tes) in the amended soils (f1 and f2) in comparison with a non-amended soil (r). ten years after the soil treatment, the particle size distribution analysis between fly ashes and their corresponding masses (fly ash þ soil particles) showed a loss or an agglomeration of finer particles. this evolution matches with the appearance of gypsum (caso4 2h2o) in fa2m instead of anhydrite (caso4), which is the major compound of fa2. this finding corresponds well with the dissolution and the lixiviation of ca, s and p included in fa2 along the f2 soil profile, generating an accumulation of these elements at 30 cm depth. however, no variation of te contaminationwas found between 0 and 25 cm depth in f2 soil except for cd. conversely, cd, pb, zn and hg enrichmentwas observed at 25 cm depth in the f1 soil, whereas no enrichmentwas observed for as. the fly ashes studied, and notably fa2, were able to reduce cd, pb and zn availability in soil and this capacity persists over the time despite their structural and chemical changes. |
Auteurs : Branchu Philippe; Joimel Sophie; Douay Francis; Lefebvre Gaëtan; Rémy Elisabeth; Béchet Béatrice; Neel Catherine; Dumat Camille; Scimia Jennifer; Schwartz Christophe Risques environnemetaux et sanitaires dans les jardins AFPP – 4ème conférence sur l’entretien des jardins végétalisés et infrastructures, 19-20 octobre 2016, Toulouse @conference{Branchu2016, title = {Risques environnemetaux et sanitaires dans les jardins}, author = {Philippe Branchu and Sophie Joimel and Francis Douay and Gaëtan Lefebvre and Elisabeth Rémy and Béatrice Béchet and Catherine Neel and Camille Dumat and Jennifer Scimia and Christophe Schwartz}, year = {2016}, date = {2016-01-01}, booktitle = {AFPP – 4ème conférence sur l’entretien des jardins végétalisés et infrastructures, 19-20 octobre 2016, Toulouse}, abstract = {L’objectif de cet exposé est de discuter de quelle façon, dans certains cas, l’état du système sol (cf. présentation de c. schwartz) peut constituer un risque pour l’environnement et/ou un risque sanitaire pour les usagers des jardins et/ou les consommateurs de légumes y poussant. la limite entre contamination (présence en concentrations anormales de substances potentiellement dangereuses) et pollution (dans le cas où ces concentrations peuvent induire une nuisance ou un risque pour l’homme et/ou l’environnement) n’étant pas définie sur le plan réglementaire en france, on se trouve parfois en situation d’incertitude que la réalisation d’une évaluation quantitative des risques sanitaires ne lève pas complètement, tant les hypothèses associées au calcul sont nombreuses. la situation des sites étudiés dans le projet jassur est discutée en termes de fertilité, de contamination en éléments traces métalliques, de biodiversité et de risques environnementaux. des exemples d’évaluations quantitatives des risques sanitaires de jardins seront présentés. enfin nous verrons de quelles façons certaines collectivités, à l’étranger, se sont appuyés sur la connaissance scientifique du système et sur le dire d’experts en cas d’incertitudes pour proposer des lignes directrices visant à la création de jardins associatifs en milieu urbain}, note = {COM}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } L’objectif de cet exposé est de discuter de quelle façon, dans certains cas, l’état du système sol (cf. présentation de c. schwartz) peut constituer un risque pour l’environnement et/ou un risque sanitaire pour les usagers des jardins et/ou les consommateurs de légumes y poussant. la limite entre contamination (présence en concentrations anormales de substances potentiellement dangereuses) et pollution (dans le cas où ces concentrations peuvent induire une nuisance ou un risque pour l’homme et/ou l’environnement) n’étant pas définie sur le plan réglementaire en france, on se trouve parfois en situation d’incertitude que la réalisation d’une évaluation quantitative des risques sanitaires ne lève pas complètement, tant les hypothèses associées au calcul sont nombreuses. la situation des sites étudiés dans le projet jassur est discutée en termes de fertilité, de contamination en éléments traces métalliques, de biodiversité et de risques environnementaux. des exemples d’évaluations quantitatives des risques sanitaires de jardins seront présentés. enfin nous verrons de quelles façons certaines collectivités, à l’étranger, se sont appuyés sur la connaissance scientifique du système et sur le dire d’experts en cas d’incertitudes pour proposer des lignes directrices visant à la création de jardins associatifs en milieu urbain |
Auteurs : Canavese Marine; Bouquet Dorine; Grenet Marie; Lebeau Thierry; Béchet Béatrice; Le-Guern Cécile; Douay Francis; Pinte Emilie; Berthier Nathalie; Branchu Philippe; Cambier Philippe; Rémy Elisabeth La qualité des sols urbains entre en politique: du désordre aux nouveaux agencements sociotechniques dans les jardins collectifs urbains Colloque "ordres et désordres au jardin", 23-24 mars 2016, Strasbourg @conference{Canavese2016, title = {La qualité des sols urbains entre en politique: du désordre aux nouveaux agencements sociotechniques dans les jardins collectifs urbains}, author = {Marine Canavese and Dorine Bouquet and Marie Grenet and Thierry Lebeau and Béatrice Béchet and Cécile Le-Guern and Francis Douay and Emilie Pinte and Nathalie Berthier and Philippe Branchu and Philippe Cambier and Elisabeth Rémy}, year = {2016}, date = {2016-01-01}, booktitle = {Colloque "ordres et désordres au jardin", 23-24 mars 2016, Strasbourg}, note = {COM}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } |
Auteurs : Cheviron Nathalie; Gattin Isabelle; Criquet Steven; Marrauld Christelle; Laval Karine; Peres Guenola; Bispo Antonio; Béguiristain Thierry; Faure Olivier; Douay Francis; Hitmi Adnane; Mougin Christian Effect of the type of contamination and land use on soil enzymatic activities: results of the french « Bioindicators program » SETAC Europe. 22-26 mai 2016, Nantes @conference{Cheviron2016, title = {Effect of the type of contamination and land use on soil enzymatic activities: results of the french « Bioindicators program »}, author = {Nathalie Cheviron and Isabelle Gattin and Steven Criquet and Christelle Marrauld and Karine Laval and Guenola Peres and Antonio Bispo and Thierry Béguiristain and Olivier Faure and Francis Douay and Adnane Hitmi and Christian Mougin}, year = {2016}, date = {2016-01-01}, booktitle = {SETAC Europe. 22-26 mai 2016, Nantes}, abstract = {Since many decades, enzymatic activities are used to assess the effects of land use changes, agricultural practices and soil contamination on soil functioning. a lot of studies point out contradictory results depending notably on the methodology used, as well as soil characteristics… to overcome the lack of consensus concerning indicators available to assess soil quality and functioning, the national research program “bioindicators” (overseen by ademe) was set up in france to develop and assay biological indicators (2006–2012). we aimed at standardizing and emphasizing the number of biochemical measurements, and developed miniaturized colorimetric methods to obtain a great enzymatic dataset. thirteen activities (from three laboratories) were measured within this programme, in 47 modalities of 13 experimental sites differing in terms of land use (corine land cover), contamination type – polycyclic aromatic hydrocarbures or metals – and pollution levels (vibrisse 8th decile). this dataset allows an analysis of the effect of atmospheric or added contamination gradients on the enzymatic activities. our objectives are to point out the sensitivity of enzymes for each type of pollution and land use, to offer the most appropriate panel for a sensible analysis. data were analysed for the all datasets, each site and each land use. our main conclusions are as follows: enzymes are more sensitive to metallic contamination than to organic ones; enzymes are good indicators of metal bioavailability; alkaline phosphatase and arylamidase are the most relevant enzymes to assess the effect of soil contamination.}, note = {ACTI}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } Since many decades, enzymatic activities are used to assess the effects of land use changes, agricultural practices and soil contamination on soil functioning. a lot of studies point out contradictory results depending notably on the methodology used, as well as soil characteristics… to overcome the lack of consensus concerning indicators available to assess soil quality and functioning, the national research program “bioindicators” (overseen by ademe) was set up in france to develop and assay biological indicators (2006–2012). we aimed at standardizing and emphasizing the number of biochemical measurements, and developed miniaturized colorimetric methods to obtain a great enzymatic dataset. thirteen activities (from three laboratories) were measured within this programme, in 47 modalities of 13 experimental sites differing in terms of land use (corine land cover), contamination type – polycyclic aromatic hydrocarbures or metals – and pollution levels (vibrisse 8th decile). this dataset allows an analysis of the effect of atmospheric or added contamination gradients on the enzymatic activities. our objectives are to point out the sensitivity of enzymes for each type of pollution and land use, to offer the most appropriate panel for a sensible analysis. data were analysed for the all datasets, each site and each land use. our main conclusions are as follows: enzymes are more sensitive to metallic contamination than to organic ones; enzymes are good indicators of metal bioavailability; alkaline phosphatase and arylamidase are the most relevant enzymes to assess the effect of soil contamination. |
Auteurs : Détriché Sébastien; Pelfrêne Aurélie; Pinte Emilie; Cheppe Gaëtan; Douay Francis Les sols urbains de la Métropole Européenne de Lille (France) : Premiers éléments de caractérisation et enjeux 13èmes Journées d’étude des sols, 4-8 juillet 2016, Louvain-la-Neuve (Belgique) @conference{Detriche2016, title = {Les sols urbains de la Métropole Européenne de Lille (France) : Premiers éléments de caractérisation et enjeux}, author = {Sébastien Détriché and Aurélie Pelfrêne and Emilie Pinte and Gaëtan Cheppe and Francis Douay}, year = {2016}, date = {2016-01-01}, booktitle = {13èmes Journées d’étude des sols, 4-8 juillet 2016, Louvain-la-Neuve (Belgique)}, abstract = {Les études se focalisant sur les environnements et les sols urbains se sont multipliées au cours des dernières décennies, notamment en raison d’une urbanisation croissante au détriment des terres agricoles. de forts enjeux environnementaux, économiques, sociaux et sanitaires sont clairement identifiés quant à la connaissance et à la maîtrise de ces espaces en termes de gestion et de planification urbaine. les sols urbains constituent un compartiment environnemental complexe, réactif et susceptible d’assurer des services écosystémiques majeurs bien que soumis à des pressions anthropiques passées et actuelles fortes. les besoins de connaissance sur la qualité de ces sols et leur distribution spatiale sont en plein développement malgré les difficultés rencontrées en raison de fortes variabilités spatiales du degré d’anthropisation des sols, de l’hétérogénéité des méthodes d’échantillonnage et d’analyse, et de l’absence fréquente de valeurs de référence nationales en milieu urbain pour la caractérisation des pollutions. la problématique de la qualité des sols urbains et plus particulièrement celle des productions légumières au sein des jardins ou de requalification de friches industrielles est particulièrement importante sur le territoire de la métropole lilloise du fait de son lourd passé industriel, de sa forte densité de population et de l’étendue des surfaces urbanisées. ce travail se propose de porter un regard croisé sur les paramètres pédologiques et physico-chimiques de sols urbains (paramètres agronomiques classiques, degré de contamination en métaux et polluants organiques, teneurs et nature des éléments grossiers...) caractérisés sur de la métropole lilloise lors de différents programmes de recherches. ces sols ont été caractérisés dans différents contextes environnementaux tels que des délaissés infrastructuraux, des friches industrielles, des jardins familiaux et partagés... ils présentent des caractéristiques en lien étroit avec les usages des espaces étudiés et ce, au regard des activités anthropiques passées et actuelles au droit des sites (activités industrielles, remaniements, apports et altération de matériaux, pratiques culturales...) et/ou de la proximité actuelle ou passée de potentielles sources de contamination (industries, infrastructures de transport, chauffage urbain...)}, note = {AFF}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } Les études se focalisant sur les environnements et les sols urbains se sont multipliées au cours des dernières décennies, notamment en raison d’une urbanisation croissante au détriment des terres agricoles. de forts enjeux environnementaux, économiques, sociaux et sanitaires sont clairement identifiés quant à la connaissance et à la maîtrise de ces espaces en termes de gestion et de planification urbaine. les sols urbains constituent un compartiment environnemental complexe, réactif et susceptible d’assurer des services écosystémiques majeurs bien que soumis à des pressions anthropiques passées et actuelles fortes. les besoins de connaissance sur la qualité de ces sols et leur distribution spatiale sont en plein développement malgré les difficultés rencontrées en raison de fortes variabilités spatiales du degré d’anthropisation des sols, de l’hétérogénéité des méthodes d’échantillonnage et d’analyse, et de l’absence fréquente de valeurs de référence nationales en milieu urbain pour la caractérisation des pollutions. la problématique de la qualité des sols urbains et plus particulièrement celle des productions légumières au sein des jardins ou de requalification de friches industrielles est particulièrement importante sur le territoire de la métropole lilloise du fait de son lourd passé industriel, de sa forte densité de population et de l’étendue des surfaces urbanisées. ce travail se propose de porter un regard croisé sur les paramètres pédologiques et physico-chimiques de sols urbains (paramètres agronomiques classiques, degré de contamination en métaux et polluants organiques, teneurs et nature des éléments grossiers...) caractérisés sur de la métropole lilloise lors de différents programmes de recherches. ces sols ont été caractérisés dans différents contextes environnementaux tels que des délaissés infrastructuraux, des friches industrielles, des jardins familiaux et partagés... ils présentent des caractéristiques en lien étroit avec les usages des espaces étudiés et ce, au regard des activités anthropiques passées et actuelles au droit des sites (activités industrielles, remaniements, apports et altération de matériaux, pratiques culturales...) et/ou de la proximité actuelle ou passée de potentielles sources de contamination (industries, infrastructures de transport, chauffage urbain...) |
Auteurs : Douay Francis; Pelfrêne Aurélie Etat des connaissances sur les sols, usages et productions potagères dans les jardins français Journée Parc Naturel Régional Scarpe - Escaut, Conseil Scientifique de l’Environnement, 2 décembre 2016, Saint-Amand-les-Eaux @conference{Douay2016, title = {Etat des connaissances sur les sols, usages et productions potagères dans les jardins français}, author = {Francis Douay and Aurélie Pelfrêne}, year = {2016}, date = {2016-01-01}, booktitle = {Journée Parc Naturel Régional Scarpe - Escaut, Conseil Scientifique de l’Environnement, 2 décembre 2016, Saint-Amand-les-Eaux}, note = {COM}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } |
Auteurs : Douay Francis; Pelfrêne Aurélie Retour d’expérience sur la problématique des jardins affectés massivement par les activités passées de Metaleurop Nord: Projet REPJAR Journée Parc Naturel Régional Scarpe - Escaut, Conseil Scientifique de l’Environnement, 2 décembre 2016, Saint-Amand-les-Eaux @conference{Douay2016a, title = {Retour d’expérience sur la problématique des jardins affectés massivement par les activités passées de Metaleurop Nord: Projet REPJAR}, author = {Francis Douay and Aurélie Pelfrêne}, year = {2016}, date = {2016-01-01}, booktitle = {Journée Parc Naturel Régional Scarpe - Escaut, Conseil Scientifique de l’Environnement, 2 décembre 2016, Saint-Amand-les-Eaux}, note = {COM}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } |
Auteurs : Fourrier Hervé; Détriché Sébastien; Douay Francis Référentiel Régional Pédologique de la région Nord-Pas de Calais : carte des pédopaysages à 1/250 000. Programme Inventaire pour la Gestion et la Conservation des Sols (IGCS) Ministère de l’Agriculture et de l’Alimentation, LGCgE-ISA Lille, Etablissement ISA du Groupe Yncréa, 288 p., @book{Fourrier2016, title = {Référentiel Régional Pédologique de la région Nord-Pas de Calais : carte des pédopaysages à 1/250 000. Programme Inventaire pour la Gestion et la Conservation des Sols (IGCS)}, author = {Hervé Fourrier and Sébastien Détriché and Francis Douay}, year = {2016}, date = {2016-01-01}, publisher = {Ministère de l’Agriculture et de l’Alimentation, LGCgE-ISA Lille, Etablissement ISA du Groupe Yncréa, 288 p.}, abstract = {Référentiel régional pédologique de la région nord-pas de calais : carte des pédopaysages à 1/250 000. programme inventaire pour la gestion et la conservation des sols (igcs)}, note = {VAL}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {book} } Référentiel régional pédologique de la région nord-pas de calais : carte des pédopaysages à 1/250 000. programme inventaire pour la gestion et la conservation des sols (igcs) |
Auteurs : Girault Frédéric; Perrier Frédéric; Poitou Charles; Isambert Aude; Théveniaut Hervé; Laperche Valérie; Clozel-Leloup Blandine; Douay Francis Effective radium concentration in topsoils contaminated by lead and zinc smelters Science of the Total Environment, 566, pp. 865-8761 @article{Girault2016, title = {Effective radium concentration in topsoils contaminated by lead and zinc smelters}, author = {Frédéric Girault and Frédéric Perrier and Charles Poitou and Aude Isambert and Hervé Théveniaut and Valérie Laperche and Blandine Clozel-Leloup and Francis Douay}, year = {2016}, date = {2016-01-01}, journal = {Science of the Total Environment}, volume = {566}, pages = {865-876}, abstract = {Trace elements (te) are indicative of industrial pollution in soils, but geochemical methods are difficult to implement in contaminated sites with large numbers of samples. therefore, measurement of soil magnetic susceptibility (ms) has been used to map te pollutions, albeit with contrasted results in some cases. effective radium concentration (ecra), product of radium concentration by the emanation factor, can be measured in a cost-effective manner in the laboratory, and could then provide a useful addition. we evaluate this possibility using 186 topsoils sampled over about 783 km(2) around two former lead and zinc smelters in northern france. the ecra values, obtained from 319 measurements, range from 0.70 +/- 0.06 to 12.53 +/- 0.49 bq.kg(-1), and are remarkably organized spatially, away from the smelters, in domains corresponding to geographical units. lead-contaminated soils, with lead concentrations above 100 mg.kg(-1) <3 km from the smelters, are characterized on average by larger peak ecra values and larger dispersion. at large scales, away from the smelters, spatial variations of ecra correlate well with spatial variations of ms, thus suggesting that, at distance larger than 5 km, variability of ms contains a significant natural component. larger ecra values are correlated with larger fine fraction and, possibly, mercury concentration. while ms is enhanced in the vicinity of the smelters and is associated with the presence of soft ferrimagnetic minerals such as magnetite, it does not correlate systematically with metal concentrations. when multiple industrial and urban sources are present, ecra mapping, thus, can help in identifying at least part of the natural spatial variability of ms. more generally, this study shows that ecra mapping provides an independent and reliable assessment of the background spatial structure which underlies the structure of a given contamination. furthermore, ecra may provide a novel index to identify soils potentially able to fix leached components. (c) 2016 elsevier b.v. all rights reserved.}, note = {ACL}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {article} } Trace elements (te) are indicative of industrial pollution in soils, but geochemical methods are difficult to implement in contaminated sites with large numbers of samples. therefore, measurement of soil magnetic susceptibility (ms) has been used to map te pollutions, albeit with contrasted results in some cases. effective radium concentration (ecra), product of radium concentration by the emanation factor, can be measured in a cost-effective manner in the laboratory, and could then provide a useful addition. we evaluate this possibility using 186 topsoils sampled over about 783 km(2) around two former lead and zinc smelters in northern france. the ecra values, obtained from 319 measurements, range from 0.70 +/- 0.06 to 12.53 +/- 0.49 bq.kg(-1), and are remarkably organized spatially, away from the smelters, in domains corresponding to geographical units. lead-contaminated soils, with lead concentrations above 100 mg.kg(-1) <3 km from the smelters, are characterized on average by larger peak ecra values and larger dispersion. at large scales, away from the smelters, spatial variations of ecra correlate well with spatial variations of ms, thus suggesting that, at distance larger than 5 km, variability of ms contains a significant natural component. larger ecra values are correlated with larger fine fraction and, possibly, mercury concentration. while ms is enhanced in the vicinity of the smelters and is associated with the presence of soft ferrimagnetic minerals such as magnetite, it does not correlate systematically with metal concentrations. when multiple industrial and urban sources are present, ecra mapping, thus, can help in identifying at least part of the natural spatial variability of ms. more generally, this study shows that ecra mapping provides an independent and reliable assessment of the background spatial structure which underlies the structure of a given contamination. furthermore, ecra may provide a novel index to identify soils potentially able to fix leached components. (c) 2016 elsevier b.v. all rights reserved. |
Auteurs : Janus Adeline; Pelfrêne Aurélie; Sahmer Karin; Heymans Sophie; Deboffe Christophe; Douay Francis; Waterlot Christophe Value of Miscanthus biochars to decrease the availability of metals in aqueous solutions International Conference Contaminated Sites 2016, 12-13 September 2016, Bratislava (Slovakia) @conference{Janus2016, title = {Value of Miscanthus biochars to decrease the availability of metals in aqueous solutions}, author = {Adeline Janus and Aurélie Pelfrêne and Karin Sahmer and Sophie Heymans and Christophe Deboffe and Francis Douay and Christophe Waterlot}, year = {2016}, date = {2016-01-01}, booktitle = {International Conference Contaminated Sites 2016, 12-13 September 2016, Bratislava (Slovakia)}, abstract = {Introduction in europe, more than 2.5 millions of potentially contaminated site a new approach: use biochars produced with biomass cultivated on agricultural soils contaminated by metals biochar production materials and methods results conclusions the pyrolysis process impacts the biochar characteristics. the biochars produced at 600 °c show the highest ssa and ph and the lowest cec. the biochars show a high efficiency to sorb the three metals, especially those produced at 600°c. the presence of metals and pahs affect the removal efficiency, notably for the biochars produced at 400°c. the high sorption capacity of miscanthus biochars confirmed these biochars as suitable sorbent for cd, pb and zn.}, note = {AFF}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } Introduction in europe, more than 2.5 millions of potentially contaminated site a new approach: use biochars produced with biomass cultivated on agricultural soils contaminated by metals biochar production materials and methods results conclusions the pyrolysis process impacts the biochar characteristics. the biochars produced at 600 °c show the highest ssa and ph and the lowest cec. the biochars show a high efficiency to sorb the three metals, especially those produced at 600°c. the presence of metals and pahs affect the removal efficiency, notably for the biochars produced at 400°c. the high sorption capacity of miscanthus biochars confirmed these biochars as suitable sorbent for cd, pb and zn. |
Auteurs : Mench Michel; Benot Marie-Lise; Castagneyrol Bastien; Marchand Lilian; Oustrière Nadège; Sæbø A; Persson T; Schröder Peter; Szulc Wieslaw; Vangronsveld Jaco; Witters Nele; Rineau François; Weyens Nele; Daniels S; Millán Rocio; Schmid Thomas; Poschenrieder Charlotte; Marmiroli Nelson; Maestri Elena; Douay Francis Intensify production, transform biomass to energy and novel goods and protect soils in Europe (INTENSE) The 5th International Conference on Soil Pollution and Remediation, 24-26 September 2016, HangZhou (China) @conference{Mench2016, title = {Intensify production, transform biomass to energy and novel goods and protect soils in Europe (INTENSE)}, author = {Michel Mench and Marie-Lise Benot and Bastien Castagneyrol and Lilian Marchand and Nadège Oustrière and A Sæbø and T Persson and Peter Schröder and Wieslaw Szulc and Jaco Vangronsveld and Nele Witters and François Rineau and Nele Weyens and S Daniels and Rocio Millán and Thomas Schmid and Charlotte Poschenrieder and Nelson Marmiroli and Elena Maestri and Francis Douay}, year = {2016}, date = {2016-01-01}, booktitle = {The 5th International Conference on Soil Pollution and Remediation, 24-26 September 2016, HangZhou (China)}, abstract = {Introduction facce surplus (sustainable and resilient agriculture for food and non-food systems) is an era-net cofund, formed in collaboration between the european commission and a partnership of 15 countries in the frame of the joint programming initiative on agriculture, food security and climate change (facce-jpi, https://www.faccejpi.com/themes/sustainable-intensification-of-agriculture/era-net-cofund-facce-surplus). facce surplus is committed to improve collaboration across the european research area in the range of diverse, but integrated, food and non-food biomass production and transformation systems, including biorefining. the intense project is one of the 14 projects selected for funding (http://faccesurplus.org/research-projects/intense/). it is responding to several of the “great challenges” for the 21st century, which are global food security, use of renewable raw materials and production of energy from biomass, for which the agricultural sector is important. intense will contribute to sustainable increase in food production, novel products for agriculture and new perspectives for european rural landscapes. future land use must embrace efficient production and utilization of biomass for improved economic, environmental and social outcomes (thewys et al 2010).}, note = {ACTI}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } Introduction facce surplus (sustainable and resilient agriculture for food and non-food systems) is an era-net cofund, formed in collaboration between the european commission and a partnership of 15 countries in the frame of the joint programming initiative on agriculture, food security and climate change (facce-jpi, https://www.faccejpi.com/themes/sustainable-intensification-of-agriculture/era-net-cofund-facce-surplus). facce surplus is committed to improve collaboration across the european research area in the range of diverse, but integrated, food and non-food biomass production and transformation systems, including biorefining. the intense project is one of the 14 projects selected for funding (http://faccesurplus.org/research-projects/intense/). it is responding to several of the “great challenges” for the 21st century, which are global food security, use of renewable raw materials and production of energy from biomass, for which the agricultural sector is important. intense will contribute to sustainable increase in food production, novel products for agriculture and new perspectives for european rural landscapes. future land use must embrace efficient production and utilization of biomass for improved economic, environmental and social outcomes (thewys et al 2010). |
Auteurs : Nsanganwimana Florien; Waterlot Christophe; Louvel Brice; Pourrut Bertrand; Douay Francis Metal, nutrient and biomass accumulation during the growing cycle of Miscanthus established on metal-contaminated soils Journal of Plant Nutrition and Soil Science, 179(2), pp. 257-2691 @article{Nsanganwimana2016, title = {Metal, nutrient and biomass accumulation during the growing cycle of Miscanthus established on metal-contaminated soils}, author = {Florien Nsanganwimana and Christophe Waterlot and Brice Louvel and Bertrand Pourrut and Francis Douay}, year = {2016}, date = {2016-01-01}, journal = {Journal of Plant Nutrition and Soil Science}, volume = {179}, number = {2}, pages = {257-269}, abstract = {The energy crop miscanthus presents high potentials for phytomanagement. its shoot yield and nutrient accumulation has been extensively characterized in uncontaminated agricultural soils, while very little is known for metal-contaminated conditions. this study aimed at assessing potential differences in dry matter and metal and nutrient accumulation of the standing aerial biomass in miscanthus (m. x giganteus) growing in situ on agricultural plots presenting different soil cd, pb, and zn concentrations. plant samplings were conducted monthly along the growing period from may to december. cadmium, pb, zn, and the concentrations of the nutrients n, p, k, ca, mg, and na were determined in leaves and stems separately. during the growing phase, the maximum dry matter was reached in early in autumn. whatever the organ, cd and zn concentrations were higher on contaminated than on uncontaminated plots. during summer and autumn, zn and pb concentrations were higher in leaves than in stems whereas cd concentrations did not significantly differ between the organs. concentrations of n, p, k, and mg decreased across the study period whereas those of ca and na increased. overall, metal and nutrient concentrations depended on plant organ and its development stage. the dry matter and nutrient accumulation patterns were not different between contaminated and uncontaminated plots. the significance of these findings is discussed in light of best phytomanagement practices and potential uses of miscanthus biomass.}, note = {ACL}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {article} } The energy crop miscanthus presents high potentials for phytomanagement. its shoot yield and nutrient accumulation has been extensively characterized in uncontaminated agricultural soils, while very little is known for metal-contaminated conditions. this study aimed at assessing potential differences in dry matter and metal and nutrient accumulation of the standing aerial biomass in miscanthus (m. x giganteus) growing in situ on agricultural plots presenting different soil cd, pb, and zn concentrations. plant samplings were conducted monthly along the growing period from may to december. cadmium, pb, zn, and the concentrations of the nutrients n, p, k, ca, mg, and na were determined in leaves and stems separately. during the growing phase, the maximum dry matter was reached in early in autumn. whatever the organ, cd and zn concentrations were higher on contaminated than on uncontaminated plots. during summer and autumn, zn and pb concentrations were higher in leaves than in stems whereas cd concentrations did not significantly differ between the organs. concentrations of n, p, k, and mg decreased across the study period whereas those of ca and na increased. overall, metal and nutrient concentrations depended on plant organ and its development stage. the dry matter and nutrient accumulation patterns were not different between contaminated and uncontaminated plots. the significance of these findings is discussed in light of best phytomanagement practices and potential uses of miscanthus biomass. |
Auteurs : Pelfrêne Aurélie; Douay Francis De l’acquisition de données environnementales et sociales à l’évaluation de l’exposition des jardiniers Journée Parc Naturel Régional Scarpe - Escaut, Conseil Scientifique de l’Environnement, 2 décembre 2016, Saint-Amand-les-Eaux @conference{Pelfrene2016b, title = {De l’acquisition de données environnementales et sociales à l’évaluation de l’exposition des jardiniers}, author = {Aurélie Pelfrêne and Francis Douay}, year = {2016}, date = {2016-01-01}, booktitle = {Journée Parc Naturel Régional Scarpe - Escaut, Conseil Scientifique de l’Environnement, 2 décembre 2016, Saint-Amand-les-Eaux}, note = {COM}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } |
Auteurs : Pelfrêne Aurélie; Grard Olivier; Heyman Christophe; Douay Francis Assessment of oral and lung bioaccessibilities of metals from smelter-impacted dust The 3rd International Symposium on Environment and Health, 14-20 August 2016, Galway (Ireland) @conference{Pelfrene2016a, title = {Assessment of oral and lung bioaccessibilities of metals from smelter-impacted dust}, author = {Aurélie Pelfrêne and Olivier Grard and Christophe Heyman and Francis Douay}, year = {2016}, date = {2016-01-01}, booktitle = {The 3rd International Symposium on Environment and Health, 14-20 August 2016, Galway (Ireland)}, note = {AFF}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } |
Auteurs : Pelfrêne Aurélie; Grard Olivier; Heyman Christophe; Triquet Judith; Douay Francis Comment continuer à consommer ses fruits et légumes cultivés dans un contexte contaminé? Retour d’expérience. Rencontres Internationales de Liessies « Les sols nourriciers », 22 septembre 2016, Liessies @conference{Pelfrene2016bb, title = {Comment continuer à consommer ses fruits et légumes cultivés dans un contexte contaminé?}, author = {Aurélie Pelfrêne and Olivier Grard and Christophe Heyman and Judith Triquet and Francis Douay}, year = {2016}, date = {2016-01-01}, booktitle = {Retour d’expérience. Rencontres Internationales de Liessies « Les sols nourriciers », 22 septembre 2016, Liessies}, note = {ACTN}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } |
Auteurs : Pourrut Bertrand; Al-Souki Karim; Liné Clarisse; Gruyer Nicolas; Dorais Martine; Douay Francis Use of multiple biomarkers to evaluate plant species suitability to manage contaminated areas International Conference on Occupational & Environmental Toxicology, 21-23 June 2016, Porto (Portugal) @conference{Pourrut2016, title = {Use of multiple biomarkers to evaluate plant species suitability to manage contaminated areas}, author = {Bertrand Pourrut and Karim Al-Souki and Clarisse Liné and Nicolas Gruyer and Martine Dorais and Francis Douay}, year = {2016}, date = {2016-01-01}, booktitle = {International Conference on Occupational & Environmental Toxicology, 21-23 June 2016, Porto (Portugal)}, abstract = {The use of plants and associated microorganisms has been increasely consider as a sustainable and cost-effective option to manage contaminated areas. phytoremediation techniques aims at using plant species to alleviate environmental and health risks induced by pollutants, and at restoring ecosystem services. suitable plant species must be tolerant to contaminants and reduce their transfer into the food chain. the selection of plant species or cultivars is mainly based on their ability to grow on contaminated soils, to stimulate organic pollutant degradation (rhizodegradation) and/or to reduce pollutants mobility (phytostabilisation) or increase pollutant uptake (phytoextraction). however, despite phytoremediation approaches are long-term techniques, only limited studies have focused on plant long-term ability to survive on contaminated areas. in this study, we investigated sub-lethal effects of contaminated soil exposure on three plant species which have been described as good candidates for phytomanagement of contaminated areas: ryegrass (lolium perenne), clover (trifolium repens) and miscanthus (miscanthus x giganteus). plants were grown on soils contaminated with heavy metals by a former lead smelter. we analyzed several biomarkers (oxidative stress, lipid peroxidation, photosynthetic pigments, dna degradation) to evaluate plant health. our results clearly demonstrated different metal tolerances among these plants. despite its potential to stabilize pollutants in soils, ryegrass plants exhibited high level of oxidative stress, lipid degradation and dna stand breaks. these results challenge the suitability of this plant for a long-term management of contaminated soils. in the other hands, miscanthus plants showed little effects of metals, event at extremely high concentrations. this confirm this plant as good candidate for phytomanagement.}, note = {ACTI}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } The use of plants and associated microorganisms has been increasely consider as a sustainable and cost-effective option to manage contaminated areas. phytoremediation techniques aims at using plant species to alleviate environmental and health risks induced by pollutants, and at restoring ecosystem services. suitable plant species must be tolerant to contaminants and reduce their transfer into the food chain. the selection of plant species or cultivars is mainly based on their ability to grow on contaminated soils, to stimulate organic pollutant degradation (rhizodegradation) and/or to reduce pollutants mobility (phytostabilisation) or increase pollutant uptake (phytoextraction). however, despite phytoremediation approaches are long-term techniques, only limited studies have focused on plant long-term ability to survive on contaminated areas. in this study, we investigated sub-lethal effects of contaminated soil exposure on three plant species which have been described as good candidates for phytomanagement of contaminated areas: ryegrass (lolium perenne), clover (trifolium repens) and miscanthus (miscanthus x giganteus). plants were grown on soils contaminated with heavy metals by a former lead smelter. we analyzed several biomarkers (oxidative stress, lipid peroxidation, photosynthetic pigments, dna degradation) to evaluate plant health. our results clearly demonstrated different metal tolerances among these plants. despite its potential to stabilize pollutants in soils, ryegrass plants exhibited high level of oxidative stress, lipid degradation and dna stand breaks. these results challenge the suitability of this plant for a long-term management of contaminated soils. in the other hands, miscanthus plants showed little effects of metals, event at extremely high concentrations. this confirm this plant as good candidate for phytomanagement. |
Auteurs : Pourrut Bertrand; Al-Souki Karim; Liné Clarisse; Gruyer Nicolas; Dorais Martine; Douay Francis Use of multiple biomarkers to evaluate plant species suitability to manage contaminated areas International Conference Contaminated Sites 2016, 12-13 September 2016, Bratislava (Slovakia) @conference{Pourrut2016a, title = {Use of multiple biomarkers to evaluate plant species suitability to manage contaminated areas}, author = {Bertrand Pourrut and Karim Al-Souki and Clarisse Liné and Nicolas Gruyer and Martine Dorais and Francis Douay}, year = {2016}, date = {2016-01-01}, booktitle = {International Conference Contaminated Sites 2016, 12-13 September 2016, Bratislava (Slovakia)}, abstract = {The use of plants and associated microorganisms has been increasely consider as a sustainable and cost-effective option to manage contaminated areas. phytomanagement is mainly based on the ability of plants to uptake (phytoextraction), stabilize (phytostabilization) or degrade (phytodegration/rhizodegradation) pollutants. it aims at reducing human and environmental risks while stimulating ecological restoration and restoring landscape. the selection of plant species or cultivars to manage contaminated sites is mainly based on their ability to stimulate organic pollutant degradation and/or to reduce pollutants mobility or increase pollutant uptake. however, despite phytoremediation approaches are long-term techniques, only limited studies have focused on plant long-term ability to survive on contaminated areas. most of studies are short-term experiments (few weeks/months) lead into greenhouses. moreover, plant health is superficially evaluated using few macroscopic markers (germination, plant growth, number of leaves…) or biomarkers at molecular scale (antioxidant enzymes, photosynthetic pigments…). this lack of concern about plant health explains the limited success of several in situ phytomanagement studies. thus, there is a need to develop alternative methods to evaluate plant long-term ability to survive on contaminated areas. here, we investigated sub-lethal effects of contaminated soil exposure on three plant species described as good candidates for phytomanagement of contaminated areas: ryegrass (lolium perenne), clover (trifolium repens) and miscanthus (miscanthus x giganteus). plants were grown during 8 weeks into pots containing a range of contaminated soils (fig. 1) collected around the former lead smelter metaleurop nord (northern france).}, note = {ACTI}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } The use of plants and associated microorganisms has been increasely consider as a sustainable and cost-effective option to manage contaminated areas. phytomanagement is mainly based on the ability of plants to uptake (phytoextraction), stabilize (phytostabilization) or degrade (phytodegration/rhizodegradation) pollutants. it aims at reducing human and environmental risks while stimulating ecological restoration and restoring landscape. the selection of plant species or cultivars to manage contaminated sites is mainly based on their ability to stimulate organic pollutant degradation and/or to reduce pollutants mobility or increase pollutant uptake. however, despite phytoremediation approaches are long-term techniques, only limited studies have focused on plant long-term ability to survive on contaminated areas. most of studies are short-term experiments (few weeks/months) lead into greenhouses. moreover, plant health is superficially evaluated using few macroscopic markers (germination, plant growth, number of leaves…) or biomarkers at molecular scale (antioxidant enzymes, photosynthetic pigments…). this lack of concern about plant health explains the limited success of several in situ phytomanagement studies. thus, there is a need to develop alternative methods to evaluate plant long-term ability to survive on contaminated areas. here, we investigated sub-lethal effects of contaminated soil exposure on three plant species described as good candidates for phytomanagement of contaminated areas: ryegrass (lolium perenne), clover (trifolium repens) and miscanthus (miscanthus x giganteus). plants were grown during 8 weeks into pots containing a range of contaminated soils (fig. 1) collected around the former lead smelter metaleurop nord (northern france). |
Auteurs : Pourrut Bertrand; Dubus Julien; Homsy Charles; Ratsizafy Irinah; Douay Francis; Soussaline Michel; Soussaline Françoise Development of an automated scoring system for plant comet assay International Conference on Occupational & Environmental Toxicology, 21-23 June 2016, Porto (Portugal) @conference{Pourrut2016b, title = {Development of an automated scoring system for plant comet assay}, author = {Bertrand Pourrut and Julien Dubus and Charles Homsy and Irinah Ratsizafy and Francis Douay and Michel Soussaline and Françoise Soussaline}, year = {2016}, date = {2016-01-01}, booktitle = {International Conference on Occupational & Environmental Toxicology, 21-23 June 2016, Porto (Portugal)}, abstract = {In ten years, the application of the comet assay has been established as one of the most interesting techniques in eco-genotoxicology. it is a rapid, sensitive and relatively inexpensive assay for measuring dna damages and repairs in individual cells. however, its use in plant studies was rather limited compared to animal studies because of (i) the difficulty to isolate intact nuclei compared to animal systems, (ii) the low throughput of current nucleus extraction, and (iii) the lack of a high throughput comet assay scoring method. in the frame of the french-norwegian project compack (2014-2017), we first developed a new nucleus extraction technique compatible with the medium-throughput comet assay (12-gel system). this new extraction method appeared to be faster, more reliable and more efficient than the so far used methods. however, the mechanical nuclei extraction generates nonuniform backgrounds with several debris that could potentially induce bias in image analysis with automated scoring systems. thus, we have optimized our nuclei extraction to reduce the presence of debris and increase background quality. meanwhile, we have worked on the automation of the scoring method which represents a technological breakthrough in plant comet assay. we have adapted the automated scoring system pathfinder™, developed by imstar, which was initially set up for human/animal cells, to score plant nuclei. our promising results open up the perspective of an automated high-throughput scoring of plant nuclei.}, note = {AFF}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } In ten years, the application of the comet assay has been established as one of the most interesting techniques in eco-genotoxicology. it is a rapid, sensitive and relatively inexpensive assay for measuring dna damages and repairs in individual cells. however, its use in plant studies was rather limited compared to animal studies because of (i) the difficulty to isolate intact nuclei compared to animal systems, (ii) the low throughput of current nucleus extraction, and (iii) the lack of a high throughput comet assay scoring method. in the frame of the french-norwegian project compack (2014-2017), we first developed a new nucleus extraction technique compatible with the medium-throughput comet assay (12-gel system). this new extraction method appeared to be faster, more reliable and more efficient than the so far used methods. however, the mechanical nuclei extraction generates nonuniform backgrounds with several debris that could potentially induce bias in image analysis with automated scoring systems. thus, we have optimized our nuclei extraction to reduce the presence of debris and increase background quality. meanwhile, we have worked on the automation of the scoring method which represents a technological breakthrough in plant comet assay. we have adapted the automated scoring system pathfinder™, developed by imstar, which was initially set up for human/animal cells, to score plant nuclei. our promising results open up the perspective of an automated high-throughput scoring of plant nuclei. |
Auteurs : Waterlot Christophe; Pelfrêne Aurélie; Douay Francis Determining the influence of the physicochemical parameters of urban soils on As availability using chemometric methods: A preliminary study Journal of Environmental Sciences, 47, pp. 183-1921 @article{Waterlot2016a, title = {Determining the influence of the physicochemical parameters of urban soils on As availability using chemometric methods: A preliminary study}, author = {Christophe Waterlot and Aurélie Pelfrêne and Francis Douay}, year = {2016}, date = {2016-01-01}, journal = {Journal of Environmental Sciences}, volume = {47}, pages = {183-192}, abstract = {An initial exploration was conducted using mathematical and statistical methods to obtain relevant information about the determination of the physicochemical parameters capable of controlling As uptake by ryegrass grown on contaminated topsoils. Concentrations of As in the soils were from 10 to 47 mg/kg, mainly in the As(V) form (57%–73%). Concentrations of As in water extracts were very low (61–700 μg/kg). It was suggested that As(III) was mainly in the uncharged species and As(V) in the charged species. Chemometric methods revealed that the values of the ratio As(III)/As(V) depended on the assimilated-phosphorus, the pseudo-total and water-extractable Fe contents and the soil pH. Arsenic concentrations measured in ryegrass shoots ranged from 119 to 1602 μg/kg. Positive linear correlations were obtained between As in ryegrass shoots and water extractable-As. The transfer coefficient of As correlated well with the ratio assimilated-phosphorus/Fe-oxides. As(III) uptake by the shoot of ryegrass was controlled by the organic matter and Fe-oxide contents.}, note = {ACL}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {article} } An initial exploration was conducted using mathematical and statistical methods to obtain relevant information about the determination of the physicochemical parameters capable of controlling As uptake by ryegrass grown on contaminated topsoils. Concentrations of As in the soils were from 10 to 47 mg/kg, mainly in the As(V) form (57%–73%). Concentrations of As in water extracts were very low (61–700 μg/kg). It was suggested that As(III) was mainly in the uncharged species and As(V) in the charged species. Chemometric methods revealed that the values of the ratio As(III)/As(V) depended on the assimilated-phosphorus, the pseudo-total and water-extractable Fe contents and the soil pH. Arsenic concentrations measured in ryegrass shoots ranged from 119 to 1602 μg/kg. Positive linear correlations were obtained between As in ryegrass shoots and water extractable-As. The transfer coefficient of As correlated well with the ratio assimilated-phosphorus/Fe-oxides. As(III) uptake by the shoot of ryegrass was controlled by the organic matter and Fe-oxide contents. |
Auteurs : Waterlot Christophe; Pruvot Christelle; Bidar Géraldine; Fritsch Clémentine; De-Vaufleury Annette; Scheifler Renaud; Douay Francis Prediction of extractable Cd, Pb and Zn in contaminated woody habitat soils using a change point detection method Pedosphere, 26(3), pp. 282-2981 @article{Waterlot2016b, title = {Prediction of extractable Cd, Pb and Zn in contaminated woody habitat soils using a change point detection method}, author = {Christophe Waterlot and Christelle Pruvot and Géraldine Bidar and Clémentine Fritsch and Annette De-Vaufleury and Renaud Scheifler and Francis Douay}, year = {2016}, date = {2016-01-01}, journal = {Pedosphere}, volume = {26}, number = {3}, pages = {282-298}, abstract = {Accumulation of heavy metals in soils poses a potential risk to plant production, which is related to availability of the metals in soil. The phytoavailability of metals is usually evaluated using extracting solutions such as salts, acids or chelates. The purpose of this study was to identify the most significant soil parameters that can be used to predict the concentrations of acetic and citric acid-extractable cadmium (Cd), lead (Pb) and zinc (Zn) in contaminated woody habitat topsoils. Multiple linear regression models were established using two analysis strategies and three sets of variables based on a dataset of 260 soil samples. The performance of these models was evaluated using statistical parameters. Cation exchange capacity, CaCO3, organic matter, assimilated P, free Al oxide, sand and the total metal concentrations appeared to be the main soil parameters governing the solubility of Cd, Pb and Zn in acetic and citric acid solutions. The results strongly suggest that the metal solubility in extracting solutions is extractable concentration-dependent since models were overall improved by incorporating a change point. This change point detection method was a powerful tool for predicting extractable Cd, Pb and Zn. Suitable predictions of extractable Cd, Pb and Zn concentrations were obtained, with correlation coefficient (adjusted r) ranging from 0.80 to 0.99, given the high complexity of the woody habitat soils studied. Therefore, the predictive models can constitute a decision-making support tool for managing phytoremediation of contaminated soils, making recommendations to control the potential bioavailability of metals. The relationships between acetic and/or citric acid-extractable concentrations and the concentrations of metals into the aboveground parts of plants need to be predicted, in order to make their temporal monitoring easier.}, note = {ACL}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {article} } Accumulation of heavy metals in soils poses a potential risk to plant production, which is related to availability of the metals in soil. The phytoavailability of metals is usually evaluated using extracting solutions such as salts, acids or chelates. The purpose of this study was to identify the most significant soil parameters that can be used to predict the concentrations of acetic and citric acid-extractable cadmium (Cd), lead (Pb) and zinc (Zn) in contaminated woody habitat topsoils. Multiple linear regression models were established using two analysis strategies and three sets of variables based on a dataset of 260 soil samples. The performance of these models was evaluated using statistical parameters. Cation exchange capacity, CaCO3, organic matter, assimilated P, free Al oxide, sand and the total metal concentrations appeared to be the main soil parameters governing the solubility of Cd, Pb and Zn in acetic and citric acid solutions. The results strongly suggest that the metal solubility in extracting solutions is extractable concentration-dependent since models were overall improved by incorporating a change point. This change point detection method was a powerful tool for predicting extractable Cd, Pb and Zn. Suitable predictions of extractable Cd, Pb and Zn concentrations were obtained, with correlation coefficient (adjusted r) ranging from 0.80 to 0.99, given the high complexity of the woody habitat soils studied. Therefore, the predictive models can constitute a decision-making support tool for managing phytoremediation of contaminated soils, making recommendations to control the potential bioavailability of metals. The relationships between acetic and/or citric acid-extractable concentrations and the concentrations of metals into the aboveground parts of plants need to be predicted, in order to make their temporal monitoring easier. |
Auteurs : Détriché Sébastien; Le-Guillou Camille; Bruneel Jean-Claude; Pinte Emilie; Douay Francis; Delbaere Denis Characterization of soils from urban amenities in the vicinity of transportation infrastructures: Examples from the Lille-Kortrijk-Tournai Eurometropolis 12th Urban Environment Symposium, 1-3 June 2015, Oslo (Norvége) @conference{Detriche2015, title = {Characterization of soils from urban amenities in the vicinity of transportation infrastructures: Examples from the Lille-Kortrijk-Tournai Eurometropolis}, author = {Sébastien Détriché and Camille Le-Guillou and Jean-Claude Bruneel and Emilie Pinte and Francis Douay and Denis Delbaere}, year = {2015}, date = {2015-01-01}, booktitle = {12th Urban Environment Symposium, 1-3 June 2015, Oslo (Norvége)}, note = {AFF}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } |
Auteurs : Firmin Stéphane; Labidi Sonia; Fontaine Joël; Verdin Anthony; Laruelle Frédéric; Tisserant Benoit; Nsanganwimana Florien; Pourrut Bertrand; Dalpé Yolande; Grandmougin-Ferjani Anne; Douay Francis; Shirali Pirouz; Lounès-Hadj-Sahraoui Anissa Arbuscular mycorrhizal fungal inoculation protects Miscanthus x giganteus against trace element toxicity in a highly metal-contaminated site Science of the Total Environment, 527-528, pp. 91-991 @article{Firmin2015, title = {Arbuscular mycorrhizal fungal inoculation protects Miscanthus x giganteus against trace element toxicity in a highly metal-contaminated site}, author = {Stéphane Firmin and Sonia Labidi and Joël Fontaine and Anthony Verdin and Frédéric Laruelle and Benoit Tisserant and Florien Nsanganwimana and Bertrand Pourrut and Yolande Dalpé and Anne Grandmougin-Ferjani and Francis Douay and Pirouz Shirali and Anissa Lounès-Hadj-Sahraoui}, year = {2015}, date = {2015-01-01}, journal = {Science of the Total Environment}, volume = {527-528}, pages = {91-99}, abstract = {Arbuscular mycorrhizal fungus (amf)-assisted phytoremediation could constitute an ecological and economic method in polluted soil rehabilitation programs. the aim of this work was to characterize the trace element (te) phytoremediation potential of mycorrhizal miscanthus × giganteus. to understand the mechanisms involved in arbuscular mycorrhizal symbiosis tolerance to te toxicity, the fatty acid compositions and several stress oxidative biomarkers were compared in the roots and leaves of miscanthus × giganteus cultivated under field conditions in either te-contaminated or control soils. tes were accumulated in greater amounts in roots, but the leaves were the organ most affected by te contamination and were characterized by a strong decrease in fatty acid contents. te-induced oxidative stress in leaves was confirmed by an increase in the lipid peroxidation biomarker malondialdehyde (mda). te contamination decreased the gssg/gsh ratio in the leaves of exposed plants, while peroxidase (po) and superoxide dismutase (sod) activities were increased in leaves and in whole plants, respectively. amf inoculation also increased root colonization in the presence of te contamination. the mycorrhizal colonization determined a decrease in sod activity in the whole plant and po activities in leaves and induced a significant increase in the fatty acid content in leaves and a decrease in mda formation in whole plants. these results suggested that mycorrhization is able to confer protection against oxidative stress induced by soil pollution. our findings suggest that mycorrhizal inoculation could be used as a bioaugmentation technique, facilitating miscanthus cultivation on highly te-contaminated soil.}, note = {ACL}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {article} } Arbuscular mycorrhizal fungus (amf)-assisted phytoremediation could constitute an ecological and economic method in polluted soil rehabilitation programs. the aim of this work was to characterize the trace element (te) phytoremediation potential of mycorrhizal miscanthus × giganteus. to understand the mechanisms involved in arbuscular mycorrhizal symbiosis tolerance to te toxicity, the fatty acid compositions and several stress oxidative biomarkers were compared in the roots and leaves of miscanthus × giganteus cultivated under field conditions in either te-contaminated or control soils. tes were accumulated in greater amounts in roots, but the leaves were the organ most affected by te contamination and were characterized by a strong decrease in fatty acid contents. te-induced oxidative stress in leaves was confirmed by an increase in the lipid peroxidation biomarker malondialdehyde (mda). te contamination decreased the gssg/gsh ratio in the leaves of exposed plants, while peroxidase (po) and superoxide dismutase (sod) activities were increased in leaves and in whole plants, respectively. amf inoculation also increased root colonization in the presence of te contamination. the mycorrhizal colonization determined a decrease in sod activity in the whole plant and po activities in leaves and induced a significant increase in the fatty acid content in leaves and a decrease in mda formation in whole plants. these results suggested that mycorrhization is able to confer protection against oxidative stress induced by soil pollution. our findings suggest that mycorrhizal inoculation could be used as a bioaugmentation technique, facilitating miscanthus cultivation on highly te-contaminated soil. |
Auteurs : Glorennec Philippe; Lucas Jean-Paul; Etchevers Anne; Oulhote Youssef; Mandin Corinne; Poupon Joël; Le-Strat Yann; Bretin Philippe; Douay Francis; Le-Bot Barbara; Le-Tertre Alain Exposition au plomb des enfants dans leur logement. Projet Plomb-Habitat (2008-2014): principaux résultats, retombées et perspectives Environnement, Risques & Santé, 14(1), pp. 28-371 @article{Glorennec2015, title = {Exposition au plomb des enfants dans leur logement. Projet Plomb-Habitat (2008-2014): principaux résultats, retombées et perspectives}, author = {Philippe Glorennec and Jean-Paul Lucas and Anne Etchevers and Youssef Oulhote and Corinne Mandin and Joël Poupon and Yann Le-Strat and Philippe Bretin and Francis Douay and Barbara Le-Bot and Alain Le-Tertre}, year = {2015}, date = {2015-01-01}, journal = {Environnement, Risques & Santé}, volume = {14}, number = {1}, pages = {28-37}, abstract = {Les niveaux d'exposition au plomb ont beaucoup baissé mais demeurent une préoccupation de santé publique du fait d'une grande prévalence de l'exposition et d'effets à faibles doses, sans seuil connu. le projet « plomb-habitat » a pour objectif de décrire les sources de plomb dans l'habitat, leur association avec la plombémie, et de tester des techniques de mesure et repérage des sources. il repose sur un souséchantillon de l'enqu^ete de prévalence du saturnisme infantile « saturn'inf ». pour la première fois en france, des mesurages environnementaux menés au sein de 484 foyers, représentatifs des 3,6 millions de logements abritant au moins un enfant âgé de six mois à six ans, ont permis de décrire la contamination en plomb dans l'eau du robinet, les poussières déposées au sol, les peintures, les aires de jeux extérieures, les parties communes et les plats et cosmétiques traditionnels. le projet plomb-habitat a également permis d'identifier les déterminants environnementaux des plombémies, d'étudier dans quelle mesure la détermination des ratios isotopiques du plomb dans les compartiments environnementaux et le sang permet d'identifier les sources d'exposition, et de comparer les informations apportées par les analyses des fractions totales et acido-solubles. l'élaboration d'un modèle de prédiction des plombémies a conduit à l'évaluation de valeurs limites en plomb dans l'eau de boisson, les poussières déposées et le sol extérieur. le corpus de données et de résultats ainsi constitué dans le cadre de plomb-habitat est aujourd'hui utilisable pour fonder les décisions publiques en vue de la poursuite des efforts de réduction des expositions au plomb.}, note = {ACLN}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {article} } Les niveaux d'exposition au plomb ont beaucoup baissé mais demeurent une préoccupation de santé publique du fait d'une grande prévalence de l'exposition et d'effets à faibles doses, sans seuil connu. le projet « plomb-habitat » a pour objectif de décrire les sources de plomb dans l'habitat, leur association avec la plombémie, et de tester des techniques de mesure et repérage des sources. il repose sur un souséchantillon de l'enqu^ete de prévalence du saturnisme infantile « saturn'inf ». pour la première fois en france, des mesurages environnementaux menés au sein de 484 foyers, représentatifs des 3,6 millions de logements abritant au moins un enfant âgé de six mois à six ans, ont permis de décrire la contamination en plomb dans l'eau du robinet, les poussières déposées au sol, les peintures, les aires de jeux extérieures, les parties communes et les plats et cosmétiques traditionnels. le projet plomb-habitat a également permis d'identifier les déterminants environnementaux des plombémies, d'étudier dans quelle mesure la détermination des ratios isotopiques du plomb dans les compartiments environnementaux et le sang permet d'identifier les sources d'exposition, et de comparer les informations apportées par les analyses des fractions totales et acido-solubles. l'élaboration d'un modèle de prédiction des plombémies a conduit à l'évaluation de valeurs limites en plomb dans l'eau de boisson, les poussières déposées et le sol extérieur. le corpus de données et de résultats ainsi constitué dans le cadre de plomb-habitat est aujourd'hui utilisable pour fonder les décisions publiques en vue de la poursuite des efforts de réduction des expositions au plomb. |
Auteurs : Janus Adeline; Pelfrêne Aurélie; Heymans Sophie; Deboffe Christophe; Douay Francis; Waterlot Christophe Elaboration, characteristics and advantages of biochars for the management of contaminated soils with a specific overview on Miscanthus biochars Journal of Environmental Management, 162, pp. 275-2891 @article{Janus2015, title = {Elaboration, characteristics and advantages of biochars for the management of contaminated soils with a specific overview on Miscanthus biochars}, author = {Adeline Janus and Aurélie Pelfrêne and Sophie Heymans and Christophe Deboffe and Francis Douay and Christophe Waterlot}, year = {2015}, date = {2015-01-01}, journal = {Journal of Environmental Management}, volume = {162}, pages = {275-289}, abstract = {Biochars are products that are rich in carbon obtained by pyrolysis processes that consist in introducing a biomass (such as wood or manure) in a closed container and heating it with little or no available air. this paper reports the impacts of pyrolysis parameters on biochar characteristics. a preliminary examination of the scientific literature revealed that the type of feedstock, the temperature, the heating rate and the gas flow were the major parameters influencing the biochar characteristics. this review highlights the multitude of biochars that can be made and shows the importance of characterizing them before their use in soils. then we assess how the input of biochars in soils can affect soil parameters. a review of the literature showed modifications on: i) the physical properties of soils (i.e. the modification in soil structure and water retention), ii) the chemical properties of soils (i.e. the modification of ph, cation exchange capacity, nutrient availability, the organic matter content) and iii) the biological properties (i.e. the changes in microbial and faunal communities). all these modifications can lead to an increase in crop productivity, which confirms the value of biochars as a soil amendment. moreover, biochars can also provide an advantage for soil remediation. indeed, biochars efficiently reduce the bioavailability of organic and inorganic pollutants. in addition, this review focuses on a specific plant that can be used to produce biochars: miscanthus, a non-wood rhizomatous c4 perennial grass. miscanthus presents advantages for biochar production due to: i) its lignocellulosic content, ii) its silicon content, which can mitigate environmental stresses (notably for plants grown on contaminated sites) and iii) the greater surface area of the miscanthus biochars compared to the biochars produced with other feedstock}, note = {ACLS}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {article} } Biochars are products that are rich in carbon obtained by pyrolysis processes that consist in introducing a biomass (such as wood or manure) in a closed container and heating it with little or no available air. this paper reports the impacts of pyrolysis parameters on biochar characteristics. a preliminary examination of the scientific literature revealed that the type of feedstock, the temperature, the heating rate and the gas flow were the major parameters influencing the biochar characteristics. this review highlights the multitude of biochars that can be made and shows the importance of characterizing them before their use in soils. then we assess how the input of biochars in soils can affect soil parameters. a review of the literature showed modifications on: i) the physical properties of soils (i.e. the modification in soil structure and water retention), ii) the chemical properties of soils (i.e. the modification of ph, cation exchange capacity, nutrient availability, the organic matter content) and iii) the biological properties (i.e. the changes in microbial and faunal communities). all these modifications can lead to an increase in crop productivity, which confirms the value of biochars as a soil amendment. moreover, biochars can also provide an advantage for soil remediation. indeed, biochars efficiently reduce the bioavailability of organic and inorganic pollutants. in addition, this review focuses on a specific plant that can be used to produce biochars: miscanthus, a non-wood rhizomatous c4 perennial grass. miscanthus presents advantages for biochar production due to: i) its lignocellulosic content, ii) its silicon content, which can mitigate environmental stresses (notably for plants grown on contaminated sites) and iii) the greater surface area of the miscanthus biochars compared to the biochars produced with other feedstock |
Auteurs : Janus Adeline; Pelfrêne Aurélie; Heymans Sophie; Deboffe Christophe; Douay Francis; Waterlot Christophe Interest of Miscanthus biochars to decrease the bioavailability of metals and PAHs in aqueous solutions Joint International Biochar Symposium “Understanding Biochar Mechanisms for Pratical Implentation”, 28–30 September 2015, Geisenheim (Allemagne) @conference{Janus2015a, title = {Interest of Miscanthus biochars to decrease the bioavailability of metals and PAHs in aqueous solutions}, author = {Adeline Janus and Aurélie Pelfrêne and Sophie Heymans and Christophe Deboffe and Francis Douay and Christophe Waterlot}, year = {2015}, date = {2015-01-01}, booktitle = {Joint International Biochar Symposium “Understanding Biochar Mechanisms for Pratical Implentation”, 28–30 September 2015, Geisenheim (Allemagne)}, note = {AFF}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } |
Auteurs : Labidi Sonia; Fontaine Joël; Laruelle Frédéric; Tisserant Benoit; Dalpé Yolande; Grandmougin-Ferjani Anne; Douay Francis; Lounès-Hadj-Sahraoui Anissa Fly ash-aided phytostabilisation of highly trace element polluted soils improves the telluric fungal biomass Applied Soil Ecology, 85, pp. 69-751 @article{Labidi2015, title = {Fly ash-aided phytostabilisation of highly trace element polluted soils improves the telluric fungal biomass}, author = {Sonia Labidi and Joël Fontaine and Frédéric Laruelle and Benoit Tisserant and Yolande Dalpé and Anne Grandmougin-Ferjani and Francis Douay and Anissa Lounès-Hadj-Sahraoui}, year = {2015}, date = {2015-01-01}, journal = {Applied Soil Ecology}, volume = {85}, pages = {69-75}, abstract = {Ten years after fly ash (fa) amendments and tree mix plantation (black locust, black alder, sycamore maple, pedunculate oak and white willow), the viability of the telluric microorganisms in a highly trace element (te) polluted topsoil was studied. previous to tree plantation, three experimental plots were set up in the field: a non-amended plot (r), an amended plot with silico-aluminous fly ash (f1) and an amended plot with sulfo-calcic fly ash (f2). the arbuscular mycorrhizal fungi (amf), saprophytic fungal and bacterial biomasses were quantified by the measure of specific lipid markers (phospholipid fatty acids (plfa) and ergosterol). the highest amf root colonization was of 18% in the sub-plot of the plot (f1). the highest plfa c16:1v5 amount (2 nmol g1 soil), reported as a marker of the amf biomass, was recorded in the fly ash amended topsoil compared to the control (r). this result was in accordance with the highest number of amf spores isolated from the sub-plot of the plot f1. saprophytic and ectomycorrhizal fungal biomasses were estimated by measuring the plfa c18:2v6,9 and ergosterol amounts in the topsoil. similarly to the plfa c16:1v5 amounts, the highest plfa c18:2v6,9 amounts (4.7 nmol g1 soil) were observed in the fly ash amended topsoil compared to the control. however, no significant effect of fly ash amendments was observed on both gram-positive and gram-negative specific plfa. this study demonstrated the usefulness of fa amendments in the assisted phytostabilisation of te polluted topsoil through the enhancement of fungal population viability.}, note = {ACL}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {article} } Ten years after fly ash (fa) amendments and tree mix plantation (black locust, black alder, sycamore maple, pedunculate oak and white willow), the viability of the telluric microorganisms in a highly trace element (te) polluted topsoil was studied. previous to tree plantation, three experimental plots were set up in the field: a non-amended plot (r), an amended plot with silico-aluminous fly ash (f1) and an amended plot with sulfo-calcic fly ash (f2). the arbuscular mycorrhizal fungi (amf), saprophytic fungal and bacterial biomasses were quantified by the measure of specific lipid markers (phospholipid fatty acids (plfa) and ergosterol). the highest amf root colonization was of 18% in the sub-plot of the plot (f1). the highest plfa c16:1v5 amount (2 nmol g1 soil), reported as a marker of the amf biomass, was recorded in the fly ash amended topsoil compared to the control (r). this result was in accordance with the highest number of amf spores isolated from the sub-plot of the plot f1. saprophytic and ectomycorrhizal fungal biomasses were estimated by measuring the plfa c18:2v6,9 and ergosterol amounts in the topsoil. similarly to the plfa c16:1v5 amounts, the highest plfa c18:2v6,9 amounts (4.7 nmol g1 soil) were observed in the fly ash amended topsoil compared to the control. however, no significant effect of fly ash amendments was observed on both gram-positive and gram-negative specific plfa. this study demonstrated the usefulness of fa amendments in the assisted phytostabilisation of te polluted topsoil through the enhancement of fungal population viability. |
Auteurs : Masson Eric; Akmalov Shamshod; Blanpain Olivier; Litot Jean-Baptiste; Melin Hélène; Dos-Santos Antonio; Durand Cyril; Leprêtre Alain; Pernin Céline; Burrow Coralie; Détriché Sébastien; Douay Francis; Leclercq Julie Environnements numériques de l’image – Images numériques de l’environnement 4e journées Big Data Mining and Visualization, 18-19 juin 2015, Lyon @conference{Masson2015, title = {Environnements numériques de l’image – Images numériques de l’environnement}, author = {Eric Masson and Shamshod Akmalov and Olivier Blanpain and Jean-Baptiste Litot and Hélène Melin and Antonio Dos-Santos and Cyril Durand and Alain Leprêtre and Céline Pernin and Coralie Burrow and Sébastien Détriché and Francis Douay and Julie Leclercq}, year = {2015}, date = {2015-01-01}, booktitle = {4e journées Big Data Mining and Visualization, 18-19 juin 2015, Lyon}, note = {ACTN}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } |
Auteurs : Nsanganwimana Florien; Pourrut Bertrand; Waterlot Christophe; Louvel Brice; Bidar Géraldine; Labidi Sonia; Fontaine Joël; Muchembled Jérôme; Lounès-Hadj-Sahraoui Anissa; Fourrier Hervé; Douay Francis Metal accumulation and shoot yield of Miscanthus × giganteus growing in contaminated agricultural soils: Insights into agronomic practices Agriculture, Ecosystems & Environment, 213, pp. 61-711 @article{Nsanganwimana2015, title = {Metal accumulation and shoot yield of Miscanthus × giganteus growing in contaminated agricultural soils: Insights into agronomic practices}, author = {Florien Nsanganwimana and Bertrand Pourrut and Christophe Waterlot and Brice Louvel and Géraldine Bidar and Sonia Labidi and Joël Fontaine and Jérôme Muchembled and Anissa Lounès-Hadj-Sahraoui and Hervé Fourrier and Francis Douay}, year = {2015}, date = {2015-01-01}, journal = {Agriculture, Ecosystems & Environment}, volume = {213}, pages = {61-71}, abstract = {The choice of agronomic practices for phytomanagement of metal-contaminated soils is of crucial importance to optimize plant biomass yields and to mitigate both environmental and health risks due to metal exposure. the present study aimed to assess the effects of agronomic practices on shoot yield and on metal (cd, pb, and zn) accumulation in the organs of the energy crop miscanthus (miscanthus × giganteus) during the first three years since the plantation on metal-contaminated agricultural soils. three miscanthus cultivars, hereafter named mis-a, mis-b and mis-i, were planted at low and high density. an inoculum of arbuscular mycorrhizal fungi-amf (glomus lpa val 1) was added during plantation, and nitrogen fertilization was applied during the third growing season. metal accumulation in miscanthus organs was determined during the second growing season, whereas shoot yields and their metal concentrations were determined during both the second and the third growing seasons. based on metal concentrations and bioconcentration factors, the three cultivars mainly accumulated metals in their roots. the shoot yields increased from 3.7 to 10.3 t dw ha−1 in the second growing season to 15.8–23.3 t dw ha−1 in third growing season. there were no or very few significant differences in metal concentrations and shoot yields within treatments comprising the same cultivar. the addition of amf inoculum increased metal (mainly cd and zn) accumulation in miscanthus organs and in the shoot yields and this was more observed in both mis-b and mis-i which presented a higher root mycorrhization level than in mis-a. shoot yields in treatments comprising different cultivars depended not on fertilization but on the interactions between cultivar and planting density, and between cultivar, planting density and amf inoculum. whatever the treatment and the sampling period, pb concentrations did not significantly differ in shoot yields. the interaction between cultivar and planting density resulted in higher cd concentrations in the yields of mis-b planted at low density during the third growing season. zn concentrations increased with fertilization in all treatments, and with the addition of the amf inoculum in mis-b and in mis-i. overall, the results demonstrated that the three cultivars could be potential candidates for coupling phytostabilization and biomass production on metal-contaminated soils.}, note = {ACL}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {article} } The choice of agronomic practices for phytomanagement of metal-contaminated soils is of crucial importance to optimize plant biomass yields and to mitigate both environmental and health risks due to metal exposure. the present study aimed to assess the effects of agronomic practices on shoot yield and on metal (cd, pb, and zn) accumulation in the organs of the energy crop miscanthus (miscanthus × giganteus) during the first three years since the plantation on metal-contaminated agricultural soils. three miscanthus cultivars, hereafter named mis-a, mis-b and mis-i, were planted at low and high density. an inoculum of arbuscular mycorrhizal fungi-amf (glomus lpa val 1) was added during plantation, and nitrogen fertilization was applied during the third growing season. metal accumulation in miscanthus organs was determined during the second growing season, whereas shoot yields and their metal concentrations were determined during both the second and the third growing seasons. based on metal concentrations and bioconcentration factors, the three cultivars mainly accumulated metals in their roots. the shoot yields increased from 3.7 to 10.3 t dw ha−1 in the second growing season to 15.8–23.3 t dw ha−1 in third growing season. there were no or very few significant differences in metal concentrations and shoot yields within treatments comprising the same cultivar. the addition of amf inoculum increased metal (mainly cd and zn) accumulation in miscanthus organs and in the shoot yields and this was more observed in both mis-b and mis-i which presented a higher root mycorrhization level than in mis-a. shoot yields in treatments comprising different cultivars depended not on fertilization but on the interactions between cultivar and planting density, and between cultivar, planting density and amf inoculum. whatever the treatment and the sampling period, pb concentrations did not significantly differ in shoot yields. the interaction between cultivar and planting density resulted in higher cd concentrations in the yields of mis-b planted at low density during the third growing season. zn concentrations increased with fertilization in all treatments, and with the addition of the amf inoculum in mis-b and in mis-i. overall, the results demonstrated that the three cultivars could be potential candidates for coupling phytostabilization and biomass production on metal-contaminated soils. |
Auteurs : Ouni Ahmed; Ghemari Chedliya; Waterlot Christophe; Ayari Anas; Douay Francis; Nasri-Ammar Karima Réponses physiologiques et comportementale chez Armadillo officinalis (Duméril, 1816) suite à une contamination du substrat par des ETM 16émes journées des sciences de la mer, December 2015, Zarzis (Tunisie) @conference{Ouni2015, title = {Réponses physiologiques et comportementale chez Armadillo officinalis (Duméril, 1816) suite à une contamination du substrat par des ETM}, author = {Ahmed Ouni and Chedliya Ghemari and Christophe Waterlot and Anas Ayari and Francis Douay and Karima Nasri-Ammar}, year = {2015}, date = {2015-01-01}, booktitle = {16émes journées des sciences de la mer, December 2015, Zarzis (Tunisie)}, abstract = {Armadillo officinalis (duméril, 1816), espèce à large répartition géographique est qualifiée de bio-accumulatrice de métaux lourds. afin de mettre en évidence les réponses physiologiques et comportementales de cette espèce vis-à-vis d’une contamination par des éléments traces métalliques (cd, pb, zn et cu), des expérimentations dans des conditions de laboratoire de température (20° c) et de photopériode (ld16:8) ont été réalisées. pour ce faire, des spécimens en repos sexuel, collectés au niveau des berges de la lagune de ghar el melh, ont été exposés durant 42 jours à des concentrations croissantes des etm cités plus haut. le taux de survie ainsi que l’évolution de la masse pondérale ont été estimés chaque semaine. par ailleurs, un test d’évitement a été réalisé afin de mettre en évidence l’ec50 de cette espèce vis-à-vis des métaux étudiés cd, pb, zn et cu, qui est égale à 2.48, 63.53, 447.63 et 234,05 ppm respectivement}, note = {AFF}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } Armadillo officinalis (duméril, 1816), espèce à large répartition géographique est qualifiée de bio-accumulatrice de métaux lourds. afin de mettre en évidence les réponses physiologiques et comportementales de cette espèce vis-à-vis d’une contamination par des éléments traces métalliques (cd, pb, zn et cu), des expérimentations dans des conditions de laboratoire de température (20° c) et de photopériode (ld16:8) ont été réalisées. pour ce faire, des spécimens en repos sexuel, collectés au niveau des berges de la lagune de ghar el melh, ont été exposés durant 42 jours à des concentrations croissantes des etm cités plus haut. le taux de survie ainsi que l’évolution de la masse pondérale ont été estimés chaque semaine. par ailleurs, un test d’évitement a été réalisé afin de mettre en évidence l’ec50 de cette espèce vis-à-vis des métaux étudiés cd, pb, zn et cu, qui est égale à 2.48, 63.53, 447.63 et 234,05 ppm respectivement |
Auteurs : Pelfrêne Aurélie; Détriché Sébastien; Douay Francis Combining spatial distribution with oral bioaccessibility of metals in smelter-impacted soils: implications for human health risk assessment Environmental Geochemistry and Health, 37(1), pp. 49-621 @article{Pelfrene2015, title = {Combining spatial distribution with oral bioaccessibility of metals in smelter-impacted soils: implications for human health risk assessment}, author = {Aurélie Pelfrêne and Sébastien Détriché and Francis Douay}, year = {2015}, date = {2015-01-01}, journal = {Environmental Geochemistry and Health}, volume = {37}, number = {1}, pages = {49-62}, abstract = {Geostatistical analysis and gis-based spatial mapping have been widely used for risk assessment of environmental pollution. the objectives of this study were to: (1) investigate the spatial variability of pseudototal concentrations of cd, pb, and zn; (2) estimate the degree of contamination on the basis of pollution indexes; and (3) combine geostatistical analysis with oral bioaccessibility to better assess the population’s exposure to metals in smelter-impacted soils. implications for human health risks were assessed by considering soil as a contaminant source, a release mechanism of contaminated soil to the hands, ingestion as an exposure route, and metal bioaccessibility. the bioaccessibility data in the gastric (g) and gastrointestinal (gi) phases were integrated into the standard hazard quotient-based risk assessment method. using pollution indices showed that the entire area studied was highly polluted in terms of soil metal concentrations. however, the spatial pattern of health risk levels did not coincide with the spatial distribution of the degree of soil contamination. introducing the bioaccessible fraction of metals from soils into the exposure calculations resulted in a substantial decrease in calculated risk (hi, hazard index) and provided a more realistic estimate of exposure to the three metals. for the highly exposed population, 46 % of the soils studied provided an hi-g > 1.0 and 15 % provided an hi-gi > 1.0, suggesting probable adverse health effects in children. the present study highlights the importance of conducting studies taking into account metal bioaccessible values in risk assessment}, note = {ACL}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {article} } Geostatistical analysis and gis-based spatial mapping have been widely used for risk assessment of environmental pollution. the objectives of this study were to: (1) investigate the spatial variability of pseudototal concentrations of cd, pb, and zn; (2) estimate the degree of contamination on the basis of pollution indexes; and (3) combine geostatistical analysis with oral bioaccessibility to better assess the population’s exposure to metals in smelter-impacted soils. implications for human health risks were assessed by considering soil as a contaminant source, a release mechanism of contaminated soil to the hands, ingestion as an exposure route, and metal bioaccessibility. the bioaccessibility data in the gastric (g) and gastrointestinal (gi) phases were integrated into the standard hazard quotient-based risk assessment method. using pollution indices showed that the entire area studied was highly polluted in terms of soil metal concentrations. however, the spatial pattern of health risk levels did not coincide with the spatial distribution of the degree of soil contamination. introducing the bioaccessible fraction of metals from soils into the exposure calculations resulted in a substantial decrease in calculated risk (hi, hazard index) and provided a more realistic estimate of exposure to the three metals. for the highly exposed population, 46 % of the soils studied provided an hi-g > 1.0 and 15 % provided an hi-gi > 1.0, suggesting probable adverse health effects in children. the present study highlights the importance of conducting studies taking into account metal bioaccessible values in risk assessment |
Auteurs : Pelfrêne Aurélie; Kleckerová Andrea; Pourrut Bertrand; Nsanganwimana Florien; Douay Francis; Waterlot Christophe Effect of miscanthus cultivation on metal fractionation and human bioaccessibility in metal-contaminated soils: Comparison between greenhouse and field experiments Environmental Science and Pollution Research, 22, pp. 3043–30541 @article{Pelfrene2015a, title = {Effect of miscanthus cultivation on metal fractionation and human bioaccessibility in metal-contaminated soils: Comparison between greenhouse and field experiments}, author = {Aurélie Pelfrêne and Andrea Kleckerová and Bertrand Pourrut and Florien Nsanganwimana and Francis Douay and Christophe Waterlot}, year = {2015}, date = {2015-01-01}, journal = {Environmental Science and Pollution Research}, volume = {22}, pages = {3043–3054}, abstract = {The in situ stabilization of metals in soils using plants with great biomass value is a promising, cost-effective, and ecologically friendly alternative to manage metal-polluted sites. the goal of phytostabilization is to reduce the bioavailable concentrations of metals in polluted soil and thus reduce the risk to the environment and human health. in this context, this study aimed at evaluating miscanthus × giganteus efficiency in phytostabilizing metals on three contaminated agricultural sites after short-term exposure under greenhouse conditions and after long-term exposure under field conditions. particular attention was paid to the influence of miscanthus cultivation on (i) cd, pb, and zn fractionation using sequential extractions and (ii) metal bioaccessibility using an in vitro gastrointestinal digestion test. data gave evidence of (i) different behaviors between the greenhouse and the field; (ii) metal redistribution in soils induced by miscanthus culture, more specifically under field conditions; (iii) higher environmental availability for cd than for pb and zn was found in both conditions; and (iv) overall, a higher bioaccessible fraction for pb (about 80 %) and cd (65–77 %) than for zn (36– 52 %) was recorded in the gastric phase, with a sharp decrease in the intestinal phase (18–35 % for cd, 5–30 % for pb, and 36–52 % for zn). compared to soils without culture, the results showed that phytostabilization using miscanthus culture provided evidence for substantial effects on oral bioaccessibility of cd, pb, and zn.}, note = {ACL}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {article} } The in situ stabilization of metals in soils using plants with great biomass value is a promising, cost-effective, and ecologically friendly alternative to manage metal-polluted sites. the goal of phytostabilization is to reduce the bioavailable concentrations of metals in polluted soil and thus reduce the risk to the environment and human health. in this context, this study aimed at evaluating miscanthus × giganteus efficiency in phytostabilizing metals on three contaminated agricultural sites after short-term exposure under greenhouse conditions and after long-term exposure under field conditions. particular attention was paid to the influence of miscanthus cultivation on (i) cd, pb, and zn fractionation using sequential extractions and (ii) metal bioaccessibility using an in vitro gastrointestinal digestion test. data gave evidence of (i) different behaviors between the greenhouse and the field; (ii) metal redistribution in soils induced by miscanthus culture, more specifically under field conditions; (iii) higher environmental availability for cd than for pb and zn was found in both conditions; and (iv) overall, a higher bioaccessible fraction for pb (about 80 %) and cd (65–77 %) than for zn (36– 52 %) was recorded in the gastric phase, with a sharp decrease in the intestinal phase (18–35 % for cd, 5–30 % for pb, and 36–52 % for zn). compared to soils without culture, the results showed that phytostabilization using miscanthus culture provided evidence for substantial effects on oral bioaccessibility of cd, pb, and zn. |
Auteurs : Pelfrêne Aurélie; Waterlot Christophe; Guérin Annie; Proix Nicolas; Richard Antoine; Douay Francis Use of an in vitro digestion method to estimate human bioaccessibility of Cd in vegetables grown in smelter-impacted soils: the influence of cooking Environmental Geochemistry and Health, 37, pp. 767-7781 @article{Pelfrene2015b, title = {Use of an in vitro digestion method to estimate human bioaccessibility of Cd in vegetables grown in smelter-impacted soils: the influence of cooking}, author = {Aurélie Pelfrêne and Christophe Waterlot and Annie Guérin and Nicolas Proix and Antoine Richard and Francis Douay}, year = {2015}, date = {2015-01-01}, journal = {Environmental Geochemistry and Health}, volume = {37}, pages = {767-778}, abstract = {Metal contamination of urban soils and homegrown vegetables has caused major concern. some studies showed that cadmium (cd) was among the most significant hazards in kitchen garden soils and prolonged exposure to this metal could cause deleterious health effects in humans. in general, most risk assessment procedures are based on total concentrations of metals in vegetables. the present study assesses human bioaccessibility of cd in vegetables cultivated in smelter-impacted kitchen garden soils. seven vegetables (radish, lettuce, french bean, carrot, leek, tomato, and potato) were considered. using the ubm protocol (unified barge bioaccessibility method), the bioaccessibility of cd was measured in raw/cooked vegetables. a considerable amount of cd was mobilized from raw vegetables during the digestion process (on average 85 % in the gastric phase and 69 % in the gastrointestinal phase), which could be attributed to a high uptake of cd during the growth of the vegetables. most cd is accumulated in the vacuoles of plant cells, except what is absorbed by the cell wall, allowing cd to be released from plant tissues under moderate conditions. cooking by the steaming process generally increased the bioaccessibility of cd in french bean, carrot, and leek. for potato, few or no significant differences of cd bioaccessibility were observed after the steaming process, while the frying process strongly decreased bioaccessibility in both phases. the estimation of metal bioaccessibility in vegetables is helpful for human health risk assessment.}, note = {ACL}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {article} } Metal contamination of urban soils and homegrown vegetables has caused major concern. some studies showed that cadmium (cd) was among the most significant hazards in kitchen garden soils and prolonged exposure to this metal could cause deleterious health effects in humans. in general, most risk assessment procedures are based on total concentrations of metals in vegetables. the present study assesses human bioaccessibility of cd in vegetables cultivated in smelter-impacted kitchen garden soils. seven vegetables (radish, lettuce, french bean, carrot, leek, tomato, and potato) were considered. using the ubm protocol (unified barge bioaccessibility method), the bioaccessibility of cd was measured in raw/cooked vegetables. a considerable amount of cd was mobilized from raw vegetables during the digestion process (on average 85 % in the gastric phase and 69 % in the gastrointestinal phase), which could be attributed to a high uptake of cd during the growth of the vegetables. most cd is accumulated in the vacuoles of plant cells, except what is absorbed by the cell wall, allowing cd to be released from plant tissues under moderate conditions. cooking by the steaming process generally increased the bioaccessibility of cd in french bean, carrot, and leek. for potato, few or no significant differences of cd bioaccessibility were observed after the steaming process, while the frying process strongly decreased bioaccessibility in both phases. the estimation of metal bioaccessibility in vegetables is helpful for human health risk assessment. |
Auteurs : Pelfrêne Aurélie; Waterlot Christophe; Proix Nicolas; Guérin Annie; Richard Antoine; Douay Francis Evaluation de l'exposition des populations en lien avec la consommation de légumes autoproduits aux alentours d’une ancienne fonderie de plomb: Contribution à l’évaluation des risques sanitaires (BioacLeg) Journée Santé Environnement Société, Région Nord – Pas de Calais, 23 juin 2015, Lille @conference{Pelfrene2015c, title = {Evaluation de l'exposition des populations en lien avec la consommation de légumes autoproduits aux alentours d’une ancienne fonderie de plomb: Contribution à l’évaluation des risques sanitaires (BioacLeg)}, author = {Aurélie Pelfrêne and Christophe Waterlot and Nicolas Proix and Annie Guérin and Antoine Richard and Francis Douay}, year = {2015}, date = {2015-01-01}, booktitle = {Journée Santé Environnement Société, Région Nord – Pas de Calais, 23 juin 2015, Lille}, note = {COM}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } |
Auteurs : Pinte Emilie; Pelfrêne Aurélie; Détriché Sébastien; Cheppe Gaetan; Consales J -N; Schwartz Christophe; Douay Francis Metallic pollutants in Lille city (Northern France) community garden soils: Contamination degree and bioavailability 12th Urban Environment Symposium, 1-3 June 2015, Oslo (Norvége) @conference{Pinte2015, title = {Metallic pollutants in Lille city (Northern France) community garden soils: Contamination degree and bioavailability}, author = {Emilie Pinte and Aurélie Pelfrêne and Sébastien Détriché and Gaetan Cheppe and J -N Consales and Christophe Schwartz and Francis Douay}, year = {2015}, date = {2015-01-01}, booktitle = {12th Urban Environment Symposium, 1-3 June 2015, Oslo (Norvége)}, note = {AFF}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } |
Auteurs : Pottiez B; Allexandre S; Craye E; Couturier F; Billaut G; Petit K; Bruyere J; Delannoy V; Louvel Brice; Douay Francis; Oste S; Werbrouck D; Siah Ali; Halama Patrice Intégration de méthodes de lutte alternative dans des systèmes de culture pour réduire de 50% l’utilisation des produits conventionnels de protection des plantes 5th International Conference on Alternative Methods of Crop Protection, 11-13 mars 2015, Lille @conference{Pottiez2015, title = {Intégration de méthodes de lutte alternative dans des systèmes de culture pour réduire de 50% l’utilisation des produits conventionnels de protection des plantes}, author = {B Pottiez and S Allexandre and E Craye and F Couturier and G Billaut and K Petit and J Bruyere and V Delannoy and Brice Louvel and Francis Douay and S Oste and D Werbrouck and Ali Siah and Patrice Halama}, year = {2015}, date = {2015-01-01}, booktitle = {5th International Conference on Alternative Methods of Crop Protection, 11-13 mars 2015, Lille}, note = {ACTI}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } |
Auteurs : Pourrut Bertrand; Pinelli Eric; Celiz-Mendiola Vanessa; Silvestre Jérôme; Douay Francis Recommendations for increasing alkaline comet assay reliability in plants Mutagenesis, 30(1), pp. 37-431 @article{Pourrut2015b, title = {Recommendations for increasing alkaline comet assay reliability in plants}, author = {Bertrand Pourrut and Eric Pinelli and Vanessa Celiz-Mendiola and Jérôme Silvestre and Francis Douay}, year = {2015}, date = {2015-01-01}, journal = {Mutagenesis}, volume = {30}, number = {1}, pages = {37-43}, abstract = {In plants, an increasing interest for the comet assay was shown in the last decade. this versatile technique appears to be promising to detect the genotoxic effect of pollutants and to monitor the environment. however, the lack of a standardised protocol and the low throughput of the assay limit its use in plants. the aims of this paper are to identify key factors affecting comet assay performance and to improve its reliability and reproducibility. we examined the effect of varying several parameters on four different plant species: broad bean (vicia faba), white clover (trifolium repens), english ryegrass (lolium perenne) and miscanthus (miscanthus x giganteus). the influence of both internal (different nucleus isolation methods, presence or absence of filtration and lysis steps) and external (room temperature, light intensity) parameters were evaluated. results clearly indicate that short chopping is more efficient to isolate nuclei than the standard slicing method. filtration and lysis steps were shown to be unnecessary and thus should be skipped. data also demonstrate that high room temperatures and light could induce dna damage in isolated nuclei. calibration tests with h2o2 or ethyl methanesulfonate revealed that a special attention should be paid to plant growing stage, leaf position and exposure duration.}, note = {ACL}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {article} } In plants, an increasing interest for the comet assay was shown in the last decade. this versatile technique appears to be promising to detect the genotoxic effect of pollutants and to monitor the environment. however, the lack of a standardised protocol and the low throughput of the assay limit its use in plants. the aims of this paper are to identify key factors affecting comet assay performance and to improve its reliability and reproducibility. we examined the effect of varying several parameters on four different plant species: broad bean (vicia faba), white clover (trifolium repens), english ryegrass (lolium perenne) and miscanthus (miscanthus x giganteus). the influence of both internal (different nucleus isolation methods, presence or absence of filtration and lysis steps) and external (room temperature, light intensity) parameters were evaluated. results clearly indicate that short chopping is more efficient to isolate nuclei than the standard slicing method. filtration and lysis steps were shown to be unnecessary and thus should be skipped. data also demonstrate that high room temperatures and light could induce dna damage in isolated nuclei. calibration tests with h2o2 or ethyl methanesulfonate revealed that a special attention should be paid to plant growing stage, leaf position and exposure duration. |
Auteurs : Pourrut Bertrand; Ratsizafy Irinah; Homsy Charles; Dubus Julien; Al-Souki Karim; Douay Francis; Soussaline Michel; Soussaline Françoise Development of an automated scoring system for plant comet assay ICAW 2015 - 11th International Comet Assay Workshop, 1- 4 September 2015, Antwerpen (Belgique) @conference{Pourrut2015bb, title = {Development of an automated scoring system for plant comet assay}, author = {Bertrand Pourrut and Irinah Ratsizafy and Charles Homsy and Julien Dubus and Karim Al-Souki and Francis Douay and Michel Soussaline and Françoise Soussaline}, year = {2015}, date = {2015-01-01}, booktitle = {ICAW 2015 - 11th International Comet Assay Workshop, 1- 4 September 2015, Antwerpen (Belgique)}, abstract = {In plants, an increasing interest for the comet assay was shown in the last decade and this versatile technique appears to be promising to detect the genotoxic effect of pollutants and to monitor the environment. however, its use in plant studies was rather limited compared to animal studies because of (i) the difficulty to isolate intact nuclei compared to animal systems and the lack of a standardized protocol, (ii) the low throughput of current nucleus extraction, and (iii) the lack of a high throughput comet assay scoring method. in order to deal with these issues, we recently identified the key steps of the comet assay on plant models and proposed an optimized protocol to increase its reliability (pourrut et al.; 2015). at the same time, in the frame of the french-norwegian project compack (2014-2017), we have worked on: -the development a new nucleus extraction technique compatible with the high-throughput comet assay scoring methods; -the automation of the scoring method based on the automated scoring system pathfinder™, developed by imstar. details and results from the preliminary experiments will be presented and discussed. major issues have been: -scoring system: specific adaptation of the automated scoring system pathfinder™ is crucial as it was initially set up for human/animal cells; -background: optimization of the protocol to reduce the presence of debris and increase background quality; -nucleus density: increase the density of nuclei is of importance to increase scoring reliability (sharma et al., 2012). in conclusion, increasing plant nucleus extraction yield and automated scoring of nuclei do represent big challenges. however, our promising preliminary results open up the perspective of an automated high-throughput scoring of plant nuclei. acknowledgements authors are grateful to the french-norwegian foundation ffs/fns, the french ministry of higher education and research, and bpi france for the financial support of the compack project}, note = {ACTI}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } In plants, an increasing interest for the comet assay was shown in the last decade and this versatile technique appears to be promising to detect the genotoxic effect of pollutants and to monitor the environment. however, its use in plant studies was rather limited compared to animal studies because of (i) the difficulty to isolate intact nuclei compared to animal systems and the lack of a standardized protocol, (ii) the low throughput of current nucleus extraction, and (iii) the lack of a high throughput comet assay scoring method. in order to deal with these issues, we recently identified the key steps of the comet assay on plant models and proposed an optimized protocol to increase its reliability (pourrut et al.; 2015). at the same time, in the frame of the french-norwegian project compack (2014-2017), we have worked on: -the development a new nucleus extraction technique compatible with the high-throughput comet assay scoring methods; -the automation of the scoring method based on the automated scoring system pathfinder™, developed by imstar. details and results from the preliminary experiments will be presented and discussed. major issues have been: -scoring system: specific adaptation of the automated scoring system pathfinder™ is crucial as it was initially set up for human/animal cells; -background: optimization of the protocol to reduce the presence of debris and increase background quality; -nucleus density: increase the density of nuclei is of importance to increase scoring reliability (sharma et al., 2012). in conclusion, increasing plant nucleus extraction yield and automated scoring of nuclei do represent big challenges. however, our promising preliminary results open up the perspective of an automated high-throughput scoring of plant nuclei. acknowledgements authors are grateful to the french-norwegian foundation ffs/fns, the french ministry of higher education and research, and bpi france for the financial support of the compack project |
Auteurs : Sahmer Karin; Deweer Caroline; Muchembled Jérôme; Santorufo Lucia; Louvel Brice; Douay Francis; Halama Patrice Utilisation d'une régression non linéaire pour des applications microbiologiques Séminaire Modal (MOdels for Data Analysis and Learning), INRIA, 13 janvier 2015, Lille @conference{Sahmer2015, title = {Utilisation d'une régression non linéaire pour des applications microbiologiques}, author = {Karin Sahmer and Caroline Deweer and Jérôme Muchembled and Lucia Santorufo and Brice Louvel and Francis Douay and Patrice Halama}, year = {2015}, date = {2015-01-01}, booktitle = {Séminaire Modal (MOdels for Data Analysis and Learning), INRIA, 13 janvier 2015, Lille}, note = {ACTN}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } |
Auteurs : Waterlot Christophe; Douay Francis Arsenic mobility and speciation in contaminated kitchen garden and lawn soils: an evaluation of water for assessment of As phytoavailability Environmental Science and Pollution Research, 22(8), pp. 6164-61751 @article{Waterlot2015b, title = {Arsenic mobility and speciation in contaminated kitchen garden and lawn soils: an evaluation of water for assessment of As phytoavailability}, author = {Christophe Waterlot and Francis Douay}, year = {2015}, date = {2015-01-01}, journal = {Environmental Science and Pollution Research}, volume = {22}, number = {8}, pages = {6164-6175}, abstract = {Emissions from primary lead smelters have been recognized as one of the mainly factor which has contributed to the contamination of soils by metals. less attention has been paid to volatile metalloids such as arsenic (as) which accompanies lead (pb) smelting activities. one of the objectives of this study was to determine the as concentrations in various extracting solutions using a collection of urban soils located no far away from two former pb and zinc plants in the north of france. the procedure for the determination of as, asiii, and asv with hydride vapor generator atomic absorption spectrometry was described in details. pseudo-total concentrations of as in the studied soils ranged from 5.3 to 65.9 mg kg-1. good correlations were found between as and lead, zinc, and cadmium concentrations in soils. these depended on the soil uses and the soil distance from the source of contamination. because the form of as may pose a health risk to human population, its speciation was determined in each urban top soils. very good correlations were found between asiii and asv versus as concentrations in soils studied, but the results did no permit to establish a relation between the location of soils and their uses. in contrast, it was shown that the highest mobility factor and lowest partitioning index values were related to the location. the mobilty of as depended on the assimilated phosphorus (p), carbonate contents, and ph. the percentages of the water-extractable as concentrations ranged from 0.3 to 3.0 % of the as concentrations in soils. very good positive correlations between water-extractable asiii and asv versus water-extractable as concentrations were obtained. it was shown that the water-extractable asiii concentrations depended on the soil uses. the results revealed that soils for which the as was the most mobile presented the highest water-extractable as concentrations. principal component analysis indicated that mechanisms related to the release of as depended on the physico-chemical parameters of the soils, particularly on the assimilated p, organic matter, and/or iron oxides/hydroxides contents. finally, the glasshouse experiments using ryegrass as plant model and three soils with similar physico-chemical parameters with regard to the pca analysis showed that the water extracting solution could be a good indicator to evaluate the as phytoavailability}, note = {ACL}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {article} } Emissions from primary lead smelters have been recognized as one of the mainly factor which has contributed to the contamination of soils by metals. less attention has been paid to volatile metalloids such as arsenic (as) which accompanies lead (pb) smelting activities. one of the objectives of this study was to determine the as concentrations in various extracting solutions using a collection of urban soils located no far away from two former pb and zinc plants in the north of france. the procedure for the determination of as, asiii, and asv with hydride vapor generator atomic absorption spectrometry was described in details. pseudo-total concentrations of as in the studied soils ranged from 5.3 to 65.9 mg kg-1. good correlations were found between as and lead, zinc, and cadmium concentrations in soils. these depended on the soil uses and the soil distance from the source of contamination. because the form of as may pose a health risk to human population, its speciation was determined in each urban top soils. very good correlations were found between asiii and asv versus as concentrations in soils studied, but the results did no permit to establish a relation between the location of soils and their uses. in contrast, it was shown that the highest mobility factor and lowest partitioning index values were related to the location. the mobilty of as depended on the assimilated phosphorus (p), carbonate contents, and ph. the percentages of the water-extractable as concentrations ranged from 0.3 to 3.0 % of the as concentrations in soils. very good positive correlations between water-extractable asiii and asv versus water-extractable as concentrations were obtained. it was shown that the water-extractable asiii concentrations depended on the soil uses. the results revealed that soils for which the as was the most mobile presented the highest water-extractable as concentrations. principal component analysis indicated that mechanisms related to the release of as depended on the physico-chemical parameters of the soils, particularly on the assimilated p, organic matter, and/or iron oxides/hydroxides contents. finally, the glasshouse experiments using ryegrass as plant model and three soils with similar physico-chemical parameters with regard to the pca analysis showed that the water extracting solution could be a good indicator to evaluate the as phytoavailability |
Auteurs : Bidar Géraldine; Louvel Brice; Proix Nicolas; Waterlot Christophe; Fourrier Hervé; Richard Antoine; Douay Francis Effets à moyen terme d’une phytostabilisation aidée sur la distribution verticale des paramètres physico-chimiques et des éléments traces métalliques dans les sols 3èmes Rencontres nationales de la Recherche sur les sites et sols pollués, ADEME, 18-19 novembre 2014, Paris @conference{Bidar2014, title = {Effets à moyen terme d’une phytostabilisation aidée sur la distribution verticale des paramètres physico-chimiques et des éléments traces métalliques dans les sols}, author = {Géraldine Bidar and Brice Louvel and Nicolas Proix and Christophe Waterlot and Hervé Fourrier and Antoine Richard and Francis Douay}, year = {2014}, date = {2014-01-01}, booktitle = {3èmes Rencontres nationales de la Recherche sur les sites et sols pollués, ADEME, 18-19 novembre 2014, Paris}, note = {ACTN}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } |
Auteurs : Demuynck Sylvain; Rosalia-Succiu Iuliana; Grumiaux Fabien; Douay Francis; Leprêtre Alain Effects of field metal-contaminated soils submitted to phytostabilisation and fly ash-aided phytostabilisation on the avoidance behaviour of the earthworm Eisenia fetida Ecotoxicology and Environmental Safety, 107, pp. 170-1771 @article{Demuynck2014, title = {Effects of field metal-contaminated soils submitted to phytostabilisation and fly ash-aided phytostabilisation on the avoidance behaviour of the earthworm Eisenia fetida}, author = {Sylvain Demuynck and Iuliana Rosalia-Succiu and Fabien Grumiaux and Francis Douay and Alain Leprêtre}, year = {2014}, date = {2014-01-01}, journal = {Ecotoxicology and Environmental Safety}, volume = {107}, pages = {170-177}, abstract = {The earthworm eisenia fetida avoidance behaviour test was used to assess the quality recovery of metal-contaminated soils from lands submitted for 10 years to remediation. soils were from plots located in the surroundings of a former lead smelter plant of northern france. metal concentrations in the soils ranged from 93 to 1231, 56 to 1424, 0.3 to 20 and 15 to 45.5 mg metal/kg dry soil for pb, zn, cd and cu, respectively. several former agricultural plots were treated either by a single phytostabilisation process involving the plantation of a tree mix or by fly ash aided-phytostabilisation. silico-aluminous or sulfo-calcic ashes used were ploughed up to a 25- to 30-cm soil depth at a rate of 23.3 kg/m2 (i.e., 6 percent w/w). e. fetida was shown to avoid significantly the 10 years ash-treated soils whose habitat function has to be considered as limited. this avoidance would relate to a change of the texture of soils induced by the addition of ashes and consisting in an increased level of fine silts together with a decreased level of clays. by contrast, afforested metal-contaminated soils appeared for e. fetida as more attractive than unplanted ones. regarding the influence of the metal contamination of the soils on e. fetida, none of the soils tested even the highest contaminated one was significantly avoided by worms. this lack of reaction would result from the low bioavailability of the metals in the soils tested. at the lights of our results and those previously published on both these ashes and these ash-treated soils, the usefulness of these soil treatments is discussed}, note = {ACL}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {article} } The earthworm eisenia fetida avoidance behaviour test was used to assess the quality recovery of metal-contaminated soils from lands submitted for 10 years to remediation. soils were from plots located in the surroundings of a former lead smelter plant of northern france. metal concentrations in the soils ranged from 93 to 1231, 56 to 1424, 0.3 to 20 and 15 to 45.5 mg metal/kg dry soil for pb, zn, cd and cu, respectively. several former agricultural plots were treated either by a single phytostabilisation process involving the plantation of a tree mix or by fly ash aided-phytostabilisation. silico-aluminous or sulfo-calcic ashes used were ploughed up to a 25- to 30-cm soil depth at a rate of 23.3 kg/m2 (i.e., 6 percent w/w). e. fetida was shown to avoid significantly the 10 years ash-treated soils whose habitat function has to be considered as limited. this avoidance would relate to a change of the texture of soils induced by the addition of ashes and consisting in an increased level of fine silts together with a decreased level of clays. by contrast, afforested metal-contaminated soils appeared for e. fetida as more attractive than unplanted ones. regarding the influence of the metal contamination of the soils on e. fetida, none of the soils tested even the highest contaminated one was significantly avoided by worms. this lack of reaction would result from the low bioavailability of the metals in the soils tested. at the lights of our results and those previously published on both these ashes and these ash-treated soils, the usefulness of these soil treatments is discussed |
Auteurs : Détriché Sébastien; Pelfrêne Aurélie; Douay Francis Combining spatial distribution with oral bioaccessibility of metals in smelter-impacted soils: Implications for human health risk assessment 30th International SEGH conference, 30 June – 4 July 2014, Newcastle upon Tyne (UK) @conference{Detriche2014, title = {Combining spatial distribution with oral bioaccessibility of metals in smelter-impacted soils: Implications for human health risk assessment}, author = {Sébastien Détriché and Aurélie Pelfrêne and Francis Douay}, year = {2014}, date = {2014-01-01}, booktitle = {30th International SEGH conference, 30 June – 4 July 2014, Newcastle upon Tyne (UK)}, note = {AFF}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } |
Auteurs : Dumat Camille; Chenot Elodie-Denise; Pourrut Bertrand; Schwartz B; Leglize P; Ouvrard S; Louvel Brice; Douay Francis; Hulot C; Marot Franck Evaluation de la qualité des plantes potagères: guide d’échantillonnage et bases de données 3èmes Rencontres nationales de la Recherche sur les sites et sols pollués, ADEME, 18-19 novembre 2014, Paris @conference{Dumat2014, title = {Evaluation de la qualité des plantes potagères: guide d’échantillonnage et bases de données}, author = {Camille Dumat and Elodie-Denise Chenot and Bertrand Pourrut and B Schwartz and P Leglize and S Ouvrard and Brice Louvel and Francis Douay and C Hulot and Franck Marot}, year = {2014}, date = {2014-01-01}, booktitle = {3èmes Rencontres nationales de la Recherche sur les sites et sols pollués, ADEME, 18-19 novembre 2014, Paris}, note = {ACTN}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } |
Auteurs : Fontaine Joël; Verdin Anthony; Firmin Stéphane; Labidi Sonia; Laruelle Frédéric; Tisserant Benoit; Grandmougin-Ferjani Anne; Dalpé Yolande; Douay Francis; Shirali Pirouz; Lounès-Hadj-Sahraoui Anissa L’inoculation mycorhizienne protège Miscanthus x giganteus contre le stress oxydant induit par la contamination métallique du sol de Metaleurop : expérimentation in situ 3èmes Rencontres nationales de la Recherche sur les sites et sols pollués, ADEME, 18-19 novembre 2014, Paris @conference{Fontaine2014, title = {L’inoculation mycorhizienne protège Miscanthus x giganteus contre le stress oxydant induit par la contamination métallique du sol de Metaleurop : expérimentation in situ}, author = {Joël Fontaine and Anthony Verdin and Stéphane Firmin and Sonia Labidi and Frédéric Laruelle and Benoit Tisserant and Anne Grandmougin-Ferjani and Yolande Dalpé and Francis Douay and Pirouz Shirali and Anissa Lounès-Hadj-Sahraoui}, year = {2014}, date = {2014-01-01}, booktitle = {3èmes Rencontres nationales de la Recherche sur les sites et sols pollués, ADEME, 18-19 novembre 2014, Paris}, note = {ACTN}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } |
Auteurs : Fourrier Hervé; Détriché Sébastien; Douay Francis Référentiel Régional Pédologique de la région Nord-Pas de Calais: Etat d’avancement Journées d’Etude des Sols, 30 juin - 4 juillet 2014, Bourget du Lac @conference{Fourrier2014, title = {Référentiel Régional Pédologique de la région Nord-Pas de Calais: Etat d’avancement}, author = {Hervé Fourrier and Sébastien Détriché and Francis Douay}, year = {2014}, date = {2014-01-01}, booktitle = {Journées d’Etude des Sols, 30 juin - 4 juillet 2014, Bourget du Lac}, note = {AFF}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } |
Auteurs : Galende Maria-A.; Kolbas Aliaksandr; Marchand Lilian; Oustrière Nadège; Nsanganwimana Florien; Douay Francis; Mench Michel Miscanthus x giganteus as a candidate for phytomanaging Cu-contaminated soils Phytoremediation of Polluted Soils, 29-30 July 2014, Vigo (Espagne) @conference{Galende2014, title = {Miscanthus x giganteus as a candidate for phytomanaging Cu-contaminated soils}, author = {Maria-A. Galende and Aliaksandr Kolbas and Lilian Marchand and Nadège Oustrière and Florien Nsanganwimana and Francis Douay and Michel Mench}, year = {2014}, date = {2014-01-01}, booktitle = {Phytoremediation of Polluted Soils, 29-30 July 2014, Vigo (Espagne)}, note = {ACTI}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } |
Auteurs : Ghemari Chedliya; Waterlot Christophe; Leclercq Julie; Douay Francis; Nasri-Ammar K Metal bioaccumulation in two species of Oniscideans: Porcellio laevis and Porcellionides pruinosus from Tunisian contaminated sites 9th International Symposium of Terrestrial Isopod Biology, 26-30 juin 2014, Poitiers @conference{Ghemari2014, title = {Metal bioaccumulation in two species of Oniscideans: Porcellio laevis and Porcellionides pruinosus from Tunisian contaminated sites}, author = {Chedliya Ghemari and Christophe Waterlot and Julie Leclercq and Francis Douay and K Nasri-Ammar}, year = {2014}, date = {2014-01-01}, booktitle = {9th International Symposium of Terrestrial Isopod Biology, 26-30 juin 2014, Poitiers}, abstract = {Terrestrial isopods that are important decomposers, are known for their resistance to high soil contamination by metal trace, making their use very helpful in biomonotoring. the aim of the present study was to compare metal bioaccumulation of two common species of porcellio laevis and porcellionides pruinosus exposed to the same contaminated soil belonging to industrial area polluted by metal trace elements in the north (1), central (3) and south (1) of tunisia. we hypothesized that these two species would accumulate metals according to their bioavailability in soil and in vegetation. these results will be discussed in relation to the soil and climatic of the different sites explored}, note = {ACTI}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } Terrestrial isopods that are important decomposers, are known for their resistance to high soil contamination by metal trace, making their use very helpful in biomonotoring. the aim of the present study was to compare metal bioaccumulation of two common species of porcellio laevis and porcellionides pruinosus exposed to the same contaminated soil belonging to industrial area polluted by metal trace elements in the north (1), central (3) and south (1) of tunisia. we hypothesized that these two species would accumulate metals according to their bioavailability in soil and in vegetation. these results will be discussed in relation to the soil and climatic of the different sites explored |
Auteurs : Girault Frédéric; Perrier Frédéric; Poitou Charles; Douay Francis; Théveniaut Hervé; Laperche Valérie; Bollinger Laurent Effective radium concentration of lead-contaminated topsoils European Geosciences Union General Assembly 2014. 27 April - 2 May 2014, Vienna (Autriche) @conference{Girault2014, title = {Effective radium concentration of lead-contaminated topsoils}, author = {Frédéric Girault and Frédéric Perrier and Charles Poitou and Francis Douay and Hervé Théveniaut and Valérie Laperche and Laurent Bollinger}, year = {2014}, date = {2014-01-01}, booktitle = {European Geosciences Union General Assembly 2014. 27 April - 2 May 2014, Vienna (Autriche)}, abstract = {As the global amount of topsoil is decreasing and its importance for agricultural purposes is increasing, the detection and quantification of metallic pollutions in topsoils has become a topical concern of the utmost importance. radium, which is generally concentrated in metal oxides and hydroxides and relatively easily leached from rock and soil, could potentially give precious information about the extent of the pollution at large spatial scales. in this study, the radon source term (effective radium concentration, ecra) of more than 300 topsoils from a leadcontaminated site in the north of france has been measured using the accumulation technique. after placing the sample in a container, sampling of the air is done using a scintillation flask after some accumulation time. radon concentration in the flask is inferred from counting in a photomultiplier 3.5 h after sampling, from which the effective radium concentration (ecra) of the soil sample is calculated, expressed in bq kg−1. this technique allows the measurement of large numbers of samples. the ecra results of the topsoils, obtained over ca. 800 km2, show remarkable spatial organization and the values are compared with the results of their chemical analyses performed at isa (lille, france) and brgm (orléans, france). the highly lead-contaminated zone (with pb concentrations larger than 250 ppm) is also relatively well circumscribed using ecra apart. indeed, ecra values of topsoils are larger in the contaminated area than outside, compared with the average regional ecra value. the mapping of ecra of topsoils at large spatial scale appears therefore as an important asset to characterize this polluted area. our ecra data are also compared with the low-field specific magnetic susceptibility (χm) and other magnetic parameters to infer some insights from the magnetic fabrics in the soil and the ecra–χm relations. relations between ecra and others metallic elements (cr, co, hg, ag) or others intrinsic characteristics (amount of sand, silt, organic matter) of the topsoils are also provided. ecra appears as a powerful parameter to characterize the spatial structure of soils, and in addition provides clues on the susceptibility to heavy metal contamination. this study opens some interesting perspectives for the understanding of the relation between radium and heavy metals in the environment.}, note = {ACTI}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } As the global amount of topsoil is decreasing and its importance for agricultural purposes is increasing, the detection and quantification of metallic pollutions in topsoils has become a topical concern of the utmost importance. radium, which is generally concentrated in metal oxides and hydroxides and relatively easily leached from rock and soil, could potentially give precious information about the extent of the pollution at large spatial scales. in this study, the radon source term (effective radium concentration, ecra) of more than 300 topsoils from a leadcontaminated site in the north of france has been measured using the accumulation technique. after placing the sample in a container, sampling of the air is done using a scintillation flask after some accumulation time. radon concentration in the flask is inferred from counting in a photomultiplier 3.5 h after sampling, from which the effective radium concentration (ecra) of the soil sample is calculated, expressed in bq kg−1. this technique allows the measurement of large numbers of samples. the ecra results of the topsoils, obtained over ca. 800 km2, show remarkable spatial organization and the values are compared with the results of their chemical analyses performed at isa (lille, france) and brgm (orléans, france). the highly lead-contaminated zone (with pb concentrations larger than 250 ppm) is also relatively well circumscribed using ecra apart. indeed, ecra values of topsoils are larger in the contaminated area than outside, compared with the average regional ecra value. the mapping of ecra of topsoils at large spatial scale appears therefore as an important asset to characterize this polluted area. our ecra data are also compared with the low-field specific magnetic susceptibility (χm) and other magnetic parameters to infer some insights from the magnetic fabrics in the soil and the ecra–χm relations. relations between ecra and others metallic elements (cr, co, hg, ag) or others intrinsic characteristics (amount of sand, silt, organic matter) of the topsoils are also provided. ecra appears as a powerful parameter to characterize the spatial structure of soils, and in addition provides clues on the susceptibility to heavy metal contamination. this study opens some interesting perspectives for the understanding of the relation between radium and heavy metals in the environment. |
Auteurs : Leclercq Julie; Pernin Céline; Louvel Brice; Demuynck Sylvain; Grumiaux Fabien; Pruvot Christelle; Douay Francis; Leprêtre Alain Contribution à la restauration d’une biodiversité dans un contexte péri-industriel fortement dégradé du Nord de la France Rever 5 : REVER et CONCILIER, 5ème journées atelier. 5-6 février 2014, Université de Rouen @conference{Leclercq2014, title = {Contribution à la restauration d’une biodiversité dans un contexte péri-industriel fortement dégradé du Nord de la France}, author = {Julie Leclercq and Céline Pernin and Brice Louvel and Sylvain Demuynck and Fabien Grumiaux and Christelle Pruvot and Francis Douay and Alain Leprêtre}, year = {2014}, date = {2014-01-01}, booktitle = {Rever 5 : REVER et CONCILIER, 5ème journées atelier. 5-6 février 2014, Université de Rouen}, abstract = {Contexte l’ancien bassin minier du nord - pas de calais, et plus particulièrement le site atelier metaleurop (réseau safir http://www.safir-network.com/site_metaleurop.html), est un secteur constitué de paysages fortement dégradés et morcelés par les activités humaines (urbanisation, activités industrielles, agriculture intensive). la forte contamination des sols en plomb, cadmium et zinc aux alentours de l’ancienne fonderie de plomb metaleurop nord (noyelles-godault) est notoirement connue. elle conduit à de profonds dysfonctionnements des sols et les concentrations en métaux des productions agricoles végétales excèdent souvent les valeurs réglementaires en vigueur pour l’alimentation humaine, voire animale. l’enjeu majeur pour les institutions en charge de la gestion de ces espaces dégradés est de contribuer à leur restauration en vue de réduire d’une part, les dangers pour les populations et l’environnement et d’autre part, de favoriser leur renaturation et une reconquête paysagère. les services de l’etat envisagent de boiser les terres agricoles les plus contaminées pour les exclure de la production alimentaire. c’est une démarche qu’avait entreprise metaleurop nord entre 1997 et 2003, date de sa fermeture. ceci s’est traduit par le boisement d’environ 85 ha parmi les terres agricoles les plus contaminées aux alentours de l’ancienne fonderie. il est projeté d’exclure toute production agricole sur les sols présentant des concentrations en plomb supérieures à 500 mg kg-1. environ 50 ha de terres labourées pourraient changer d’usage et être boisées. objectif général en octobre 2012, un travail de thèse a débuté avec pour objectif de contribuer au développement du phytomanagement sur des sols fortement contaminés par des métaux. il s’agit de favoriser une renaturation et une biodiversité fonctionnelle des sols les plus contaminés en s’inspirant du retour d’expériences acquises sur les sols anciennement boisés. la fonctionnalité des sols sera observée à différentes échelles au regard de différents indicateurs physico-chimiques et biologiques. matériel et méthode plusieurs dispositifs expérimentaux sont mis en place en vue : •de comparer la vitesse de dégradation des matières organiques sur 4 parcelles boisées (mono/pluri espèces, amendements minéraux des sols ou non) à l’aide de 256 sacs de litière et suivi de la pédofaune ; •d’évaluer la restauration d’une connectivité écologique au travers de 6 modalités (bois raméal fragmenté, miscanthus, paille de céréale, feuilles mortes, bâche plastique et témoin) : inventaire mensuel de la pédofaune (grands groupes taxonomiques), suivi de la dégradation des matières organiques et des activités enzymatiques des sols… •d’examiner différents aménagements paysagers sur ce secteur dégradé à l’aide d’un panel d’indicateurs biologiques et physico-chimiques des sols. premiers résultats il a été mis en évidence : une mosaïque de paysages (plantations arborées, bois, cultures, zones résidentielles et d’activités économiques) entre lesquels la transition est souvent brutale ; des caractéristiques pédologiques variables notamment en termes de teneurs en carbonates, d’engorgement des sols par la nappe phréatique et de degré de contamination ; des communautés faunistiques perturbées, bien que la richesse spécifique de certains groupes soit élevée (carabes) ; et aussi l’absence de micro-habitats et de continuité écologique. les premiers résultats seront présentés et discutés.}, note = {ACTN}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } Contexte l’ancien bassin minier du nord - pas de calais, et plus particulièrement le site atelier metaleurop (réseau safir http://www.safir-network.com/site_metaleurop.html), est un secteur constitué de paysages fortement dégradés et morcelés par les activités humaines (urbanisation, activités industrielles, agriculture intensive). la forte contamination des sols en plomb, cadmium et zinc aux alentours de l’ancienne fonderie de plomb metaleurop nord (noyelles-godault) est notoirement connue. elle conduit à de profonds dysfonctionnements des sols et les concentrations en métaux des productions agricoles végétales excèdent souvent les valeurs réglementaires en vigueur pour l’alimentation humaine, voire animale. l’enjeu majeur pour les institutions en charge de la gestion de ces espaces dégradés est de contribuer à leur restauration en vue de réduire d’une part, les dangers pour les populations et l’environnement et d’autre part, de favoriser leur renaturation et une reconquête paysagère. les services de l’etat envisagent de boiser les terres agricoles les plus contaminées pour les exclure de la production alimentaire. c’est une démarche qu’avait entreprise metaleurop nord entre 1997 et 2003, date de sa fermeture. ceci s’est traduit par le boisement d’environ 85 ha parmi les terres agricoles les plus contaminées aux alentours de l’ancienne fonderie. il est projeté d’exclure toute production agricole sur les sols présentant des concentrations en plomb supérieures à 500 mg kg-1. environ 50 ha de terres labourées pourraient changer d’usage et être boisées. objectif général en octobre 2012, un travail de thèse a débuté avec pour objectif de contribuer au développement du phytomanagement sur des sols fortement contaminés par des métaux. il s’agit de favoriser une renaturation et une biodiversité fonctionnelle des sols les plus contaminés en s’inspirant du retour d’expériences acquises sur les sols anciennement boisés. la fonctionnalité des sols sera observée à différentes échelles au regard de différents indicateurs physico-chimiques et biologiques. matériel et méthode plusieurs dispositifs expérimentaux sont mis en place en vue : •de comparer la vitesse de dégradation des matières organiques sur 4 parcelles boisées (mono/pluri espèces, amendements minéraux des sols ou non) à l’aide de 256 sacs de litière et suivi de la pédofaune ; •d’évaluer la restauration d’une connectivité écologique au travers de 6 modalités (bois raméal fragmenté, miscanthus, paille de céréale, feuilles mortes, bâche plastique et témoin) : inventaire mensuel de la pédofaune (grands groupes taxonomiques), suivi de la dégradation des matières organiques et des activités enzymatiques des sols… •d’examiner différents aménagements paysagers sur ce secteur dégradé à l’aide d’un panel d’indicateurs biologiques et physico-chimiques des sols. premiers résultats il a été mis en évidence : une mosaïque de paysages (plantations arborées, bois, cultures, zones résidentielles et d’activités économiques) entre lesquels la transition est souvent brutale ; des caractéristiques pédologiques variables notamment en termes de teneurs en carbonates, d’engorgement des sols par la nappe phréatique et de degré de contamination ; des communautés faunistiques perturbées, bien que la richesse spécifique de certains groupes soit élevée (carabes) ; et aussi l’absence de micro-habitats et de continuité écologique. les premiers résultats seront présentés et discutés. |
Auteurs : Leclercq Julie; Santorufo Lucia; Pernin Céline; Demuynck Sylvain; Louvel Brice; Grumiaux Fabien; Détriché Sébastien; Douay Francis; Leprêtre Alain Can mulching boost ecological connectivity between different management options in a disturbed environment? First Global Soil Biodiversity Conference - Assessing soil biodiversity and its role for ecosystem services, 2-5 décembre 2014, Dijon @conference{Leclercq2014a, title = {Can mulching boost ecological connectivity between different management options in a disturbed environment?}, author = {Julie Leclercq and Lucia Santorufo and Céline Pernin and Sylvain Demuynck and Brice Louvel and Fabien Grumiaux and Sébastien Détriché and Francis Douay and Alain Leprêtre}, year = {2014}, date = {2014-01-01}, booktitle = {First Global Soil Biodiversity Conference - Assessing soil biodiversity and its role for ecosystem services, 2-5 décembre 2014, Dijon}, abstract = {Today, rare are unfragmented habitats. the landscapes are transformed by human activities and species are threatened: isolation, downsizing, reduced trade between populations... the former coal mining area of northern france is not an exception: urbanization, industry, intensive agriculture and metal pollution are the main causes of land fragmentation and disturbance. it is important to look for ways to reconnect these isolated and degraded areas. the present study aimed at evaluating the attractivity of different kind of mulches and their efficiency to promote recolonization of isolated and degrades areas. since march 2013, 18 experimental rows (6m long x 0.5 m width) of mulch were installed between two wood plots (pluri and monospecific plantations) on a surface area which stretch on 4400 m². five kind of mulch (ramial chipped wood (rcw), dead leaves, chopped miscanthus, chopped wheat straw and canvas cover) and a control (bare soil) were tested (3 replicates each). the control consisted in 3 replicated of bare soil. each mulch was characterized by the loss of biomass with litter bag technique, metal concentrations, temporal monitoring of enzymatic activities and organic matter, etc. the attendance of these mulches by pedofauna was followed using both interception and emergence traps. the formers are wet pitfall traps revealing the activity intensity of aboveground fauna. the latter track imagos of soil insects. the trapping of pedofauna was carried out from april to july 2014 using 90 pitfall traps and 78 soil emergence traps (no trap on canvas cover). a general inventory of pedofauna trapped was made, focusing on two groups models: groundbeetles (carabidae) and woodlice (isopoda), which belong to two different trophic groups, predators and saprophageous. first results show that mulches welcome more organisms than bare soil. leaves and rcw are the modalities with the highest abundances. more detailed results will be presented and discussed.}, note = {ACTI}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } Today, rare are unfragmented habitats. the landscapes are transformed by human activities and species are threatened: isolation, downsizing, reduced trade between populations... the former coal mining area of northern france is not an exception: urbanization, industry, intensive agriculture and metal pollution are the main causes of land fragmentation and disturbance. it is important to look for ways to reconnect these isolated and degraded areas. the present study aimed at evaluating the attractivity of different kind of mulches and their efficiency to promote recolonization of isolated and degrades areas. since march 2013, 18 experimental rows (6m long x 0.5 m width) of mulch were installed between two wood plots (pluri and monospecific plantations) on a surface area which stretch on 4400 m². five kind of mulch (ramial chipped wood (rcw), dead leaves, chopped miscanthus, chopped wheat straw and canvas cover) and a control (bare soil) were tested (3 replicates each). the control consisted in 3 replicated of bare soil. each mulch was characterized by the loss of biomass with litter bag technique, metal concentrations, temporal monitoring of enzymatic activities and organic matter, etc. the attendance of these mulches by pedofauna was followed using both interception and emergence traps. the formers are wet pitfall traps revealing the activity intensity of aboveground fauna. the latter track imagos of soil insects. the trapping of pedofauna was carried out from april to july 2014 using 90 pitfall traps and 78 soil emergence traps (no trap on canvas cover). a general inventory of pedofauna trapped was made, focusing on two groups models: groundbeetles (carabidae) and woodlice (isopoda), which belong to two different trophic groups, predators and saprophageous. first results show that mulches welcome more organisms than bare soil. leaves and rcw are the modalities with the highest abundances. more detailed results will be presented and discussed. |
Auteurs : Leclercq Julie; Santorufo Lucia; Pernin Céline; Demuynck Sylvain; Louvel Brice; Grumiaux Fabien; Détriché Sébastien; Douay Francis; Leprêtre Alain The use of indicators to characterize the functioning of soils strongly affected by metallurgical activities First Global Soil Biodiversity Conference - Assessing soil biodiversity and its role for ecosystem services, 2-5 décembre 2014, Dijon @conference{Leclercq2014b, title = {The use of indicators to characterize the functioning of soils strongly affected by metallurgical activities}, author = {Julie Leclercq and Lucia Santorufo and Céline Pernin and Sylvain Demuynck and Brice Louvel and Fabien Grumiaux and Sébastien Détriché and Francis Douay and Alain Leprêtre}, year = {2014}, date = {2014-01-01}, booktitle = {First Global Soil Biodiversity Conference - Assessing soil biodiversity and its role for ecosystem services, 2-5 décembre 2014, Dijon}, abstract = {The former coal mining field of northern france has been heavily disturbed due to human activities (urbanization, industry and intensive agriculture). this is particularly the case around a lead smelter (metaleurop nord) that emitted into the atmosphere until 2003 considerable quantities of metals. in order to restore this degraded area and to help reducing the environmental hazard, the most contaminated sites were afforested started in 1997. thus, 85 ha were planted around this former industrial plant creating a “green ring”. different tree species and different management options were established, but no study has been conducted on the effects of different tree species and managements on soil quality and functionality. the main aim of this study was to assess soil functionality of several parcels, differing by vegetation cover (species of trees, planting density, etc.), using soil physico-chemical and biological indicators. results would allow proposing a management for the restoration of these degraded environments. the study site consists of 12 plots: nine wooded plots (plantations of ash, poplar, maple or mixed plantations, with or without soil amendment) and three reference soils (agricultural, hedgerow, forest). the general status of trees and the truck diameters measurements were made. in addition, the topsoil was characterized by texture, bulk density, ph, cec, trace metal concentrations, etc. several soil biological indicators belonging to different functional and trophic groups were selected: microbial activity, ergosterol quantification (as estimation of fungal biomass), enzyme activities (laccase and urease) and several groups of pedofauna. indeed, the imagos emergence of soil insects was monitoring. it was conducted from mid-april to july 2014 using three soil emergence traps per plot.}, note = {ACTI}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } The former coal mining field of northern france has been heavily disturbed due to human activities (urbanization, industry and intensive agriculture). this is particularly the case around a lead smelter (metaleurop nord) that emitted into the atmosphere until 2003 considerable quantities of metals. in order to restore this degraded area and to help reducing the environmental hazard, the most contaminated sites were afforested started in 1997. thus, 85 ha were planted around this former industrial plant creating a “green ring”. different tree species and different management options were established, but no study has been conducted on the effects of different tree species and managements on soil quality and functionality. the main aim of this study was to assess soil functionality of several parcels, differing by vegetation cover (species of trees, planting density, etc.), using soil physico-chemical and biological indicators. results would allow proposing a management for the restoration of these degraded environments. the study site consists of 12 plots: nine wooded plots (plantations of ash, poplar, maple or mixed plantations, with or without soil amendment) and three reference soils (agricultural, hedgerow, forest). the general status of trees and the truck diameters measurements were made. in addition, the topsoil was characterized by texture, bulk density, ph, cec, trace metal concentrations, etc. several soil biological indicators belonging to different functional and trophic groups were selected: microbial activity, ergosterol quantification (as estimation of fungal biomass), enzyme activities (laccase and urease) and several groups of pedofauna. indeed, the imagos emergence of soil insects was monitoring. it was conducted from mid-april to july 2014 using three soil emergence traps per plot. |
Auteurs : Leclercq Julie; Santorufo Lucia; Pernin Céline; Demuynck Sylvain; Louvel Brice; Grumiaux Fabien; Douay Francis; Leprêtre Alain Le mulch de miscanthus peut-il présenter un intérêt pour la pédofaune dans un contexte dégradé et contaminé par les éléments traces métalliques? 3èmes Rencontres nationales de la Recherche sur les sites et sols pollués, ADEME, 18-19 novembre 2014, Paris @conference{Leclercq2014c, title = {Le mulch de miscanthus peut-il présenter un intérêt pour la pédofaune dans un contexte dégradé et contaminé par les éléments traces métalliques?}, author = {Julie Leclercq and Lucia Santorufo and Céline Pernin and Sylvain Demuynck and Brice Louvel and Fabien Grumiaux and Francis Douay and Alain Leprêtre}, year = {2014}, date = {2014-01-01}, booktitle = {3èmes Rencontres nationales de la Recherche sur les sites et sols pollués, ADEME, 18-19 novembre 2014, Paris}, abstract = {L’urbanisation, les activités industrielles, la pollution métallique, l’agriculture intensive sont les causes principales de la fragmentation et de la perturbation des sols dans l’ancien bassin minier de la région nord-pas de calais. ceux-ci induisent une perte de biodiversité et le fonctionnement du sol s’en trouve altéré. l’objectif de ce travail est d’évaluer l’intérêt du mulch de miscanthus x giganteus pour constituer un milieu attractif pour la pédofaune et favoriser la recolonisation des sols dégradés. en mars 2013, une expérimentation a été mise en place sur deux parcelles boisées. trois modalités de mulch (miscanthus, bois raméal fragmenté (brf), feuilles mortes) et un témoin (sol nu) ont été caractérisées en termes de concentrations en cadmium (cd), plomb (pb) et zinc (zn) ainsi que par les abondances moyennes et totales de la macrofaune. le mulch de miscanthus, bien que moins colonisé par la pédofaune par rapport aux feuilles ou au brf, est toutefois plus favorable qu’un sol nu. il présente des teneurs en cd, pb et zn très inférieures à celles du brf et des feuilles. le miscanthus apparaît alors comme une solution intermédiaire qui pourrait présenter un intérêt pour la pédofaune dans les réflexions de restauration de sols dégradés.}, note = {ACTN}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } L’urbanisation, les activités industrielles, la pollution métallique, l’agriculture intensive sont les causes principales de la fragmentation et de la perturbation des sols dans l’ancien bassin minier de la région nord-pas de calais. ceux-ci induisent une perte de biodiversité et le fonctionnement du sol s’en trouve altéré. l’objectif de ce travail est d’évaluer l’intérêt du mulch de miscanthus x giganteus pour constituer un milieu attractif pour la pédofaune et favoriser la recolonisation des sols dégradés. en mars 2013, une expérimentation a été mise en place sur deux parcelles boisées. trois modalités de mulch (miscanthus, bois raméal fragmenté (brf), feuilles mortes) et un témoin (sol nu) ont été caractérisées en termes de concentrations en cadmium (cd), plomb (pb) et zinc (zn) ainsi que par les abondances moyennes et totales de la macrofaune. le mulch de miscanthus, bien que moins colonisé par la pédofaune par rapport aux feuilles ou au brf, est toutefois plus favorable qu’un sol nu. il présente des teneurs en cd, pb et zn très inférieures à celles du brf et des feuilles. le miscanthus apparaît alors comme une solution intermédiaire qui pourrait présenter un intérêt pour la pédofaune dans les réflexions de restauration de sols dégradés. |
Auteurs : Le-Guillou Camille; Détriché Sébastien; Bruneel Jean-Claude; Douay Francis; Delbaere Denis Caractérisation des sols de délaissés infrastructuraux : exemple de l’Eurométropole Lille – Courtrai – Tournai Gestion et requalification durable des sites et sols pollués : Expériences en Nord - Pas de Calais, 25-26 septembre 2014, Villeneuve d’Ascq @conference{Le-Guillou2014, title = {Caractérisation des sols de délaissés infrastructuraux : exemple de l’Eurométropole Lille – Courtrai – Tournai}, author = {Camille Le-Guillou and Sébastien Détriché and Jean-Claude Bruneel and Francis Douay and Denis Delbaere}, year = {2014}, date = {2014-01-01}, booktitle = {Gestion et requalification durable des sites et sols pollués : Expériences en Nord - Pas de Calais, 25-26 septembre 2014, Villeneuve d’Ascq}, note = {COM}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } |
Auteurs : Louvel Brice; Fourrier Hervé; Douay Francis Influence de la phytostabilisation aidée sur les activités microbiennes dans des sols contaminés par des éléments traces métalliques 3èmes Rencontres nationales de la Recherche sur les sites et sols pollués, ADEME, 18-19 novembre 2014, Paris @conference{Louvel2014, title = {Influence de la phytostabilisation aidée sur les activités microbiennes dans des sols contaminés par des éléments traces métalliques}, author = {Brice Louvel and Hervé Fourrier and Francis Douay}, year = {2014}, date = {2014-01-01}, booktitle = {3èmes Rencontres nationales de la Recherche sur les sites et sols pollués, ADEME, 18-19 novembre 2014, Paris}, note = {ACTN}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } |
Auteurs : Marot Franck; Denys G; Cardenas G; Tack K; Douay Francis; Dumat Camille; Schwartz Christophe; Elreedy S; Gobron V; Dinant S; Jeannée Nicolas; Pinet Claire; Prost R; Pascal M; Lemaitre N; Leprieur F; Mercat-Rommens C; Barbaste Mireille Guide d’échantillonnage de plantes potagères dans le cadre de diagnostics environnementaux (2ème édition) ADEME - INERIS @techreport{Marot2014, title = {Guide d’échantillonnage de plantes potagères dans le cadre de diagnostics environnementaux (2ème édition)}, author = {Franck Marot and G Denys and G Cardenas and K Tack and Francis Douay and Camille Dumat and Christophe Schwartz and S Elreedy and V Gobron and S Dinant and Nicolas Jeannée and Claire Pinet and R Prost and M Pascal and N Lemaitre and F Leprieur and C Mercat-Rommens and Mireille Barbaste}, year = {2014}, date = {2014-01-01}, institution = {ADEME - INERIS}, abstract = {L’objectif principal de ce guide est de proposer une méthode d’échantillonnage de plantes potagères permettant de disposer d’espèces végétales puis de données analytiques représentatives des situations environnementales rencontrées. ce guide pourra être utile à la conduite d’études portant sur l’évaluation, à partir de prélèvements, de la qualité sanitaire de productions potagères consommées par l’homme et cultivées dans un environnement potentiellement pollué par une installation industrielle (actuelle ou ancienne), en situation de pollution chronique ou accidentelle. il s’applique pour interpréter directement l’état de contamination de ce milieu (les plantes potagères) au regard des valeurs réglementaires ou de référence et également pour engager une démarche basée sur la quantification des risques sanitaires dans le cas où ce milieu est retenu comme voie d’exposition. ce guide apporte aussi une démarche méthodologique visant à évaluer la contribution de l’installation industrielle étudiée sur une éventuelle contamination des cultures potagères. cela explique qu’il aborde également (sans les développer) l’échantillonnage d’autres milieux que les seules plantes potagères (e.g. eau, air, sol), ces milieux pouvant être impliqués dans les transferts de polluants vers les végétaux. la méthodologie proposée peut également être utilisée pour d’autres suivis de rejets de polluants comme les axes routiers ou autoroutiers, voire même en dehors de tout contexte de pollution connue, lorsque l’objectif consiste simplement à caractériser la qualité sanitaire de productions potagères.}, note = {OV}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {techreport} } L’objectif principal de ce guide est de proposer une méthode d’échantillonnage de plantes potagères permettant de disposer d’espèces végétales puis de données analytiques représentatives des situations environnementales rencontrées. ce guide pourra être utile à la conduite d’études portant sur l’évaluation, à partir de prélèvements, de la qualité sanitaire de productions potagères consommées par l’homme et cultivées dans un environnement potentiellement pollué par une installation industrielle (actuelle ou ancienne), en situation de pollution chronique ou accidentelle. il s’applique pour interpréter directement l’état de contamination de ce milieu (les plantes potagères) au regard des valeurs réglementaires ou de référence et également pour engager une démarche basée sur la quantification des risques sanitaires dans le cas où ce milieu est retenu comme voie d’exposition. ce guide apporte aussi une démarche méthodologique visant à évaluer la contribution de l’installation industrielle étudiée sur une éventuelle contamination des cultures potagères. cela explique qu’il aborde également (sans les développer) l’échantillonnage d’autres milieux que les seules plantes potagères (e.g. eau, air, sol), ces milieux pouvant être impliqués dans les transferts de polluants vers les végétaux. la méthodologie proposée peut également être utilisée pour d’autres suivis de rejets de polluants comme les axes routiers ou autoroutiers, voire même en dehors de tout contexte de pollution connue, lorsque l’objectif consiste simplement à caractériser la qualité sanitaire de productions potagères. |
Auteurs : Nsanganwimana Florien; Marchand Lilian; Douay Francis; Mench Michel Arundo donax L., a candidate for phytomanaging water and soils Contaminated by trace elements and producing plant-based feedstock. A review International Journal of Phytoremediation, 16(10), pp. 982-10171 @article{Nsanganwimana2014b, title = {Arundo donax L., a candidate for phytomanaging water and soils Contaminated by trace elements and producing plant-based feedstock. A review}, author = {Florien Nsanganwimana and Lilian Marchand and Francis Douay and Michel Mench}, year = {2014}, date = {2014-01-01}, journal = {International Journal of Phytoremediation}, volume = {16}, number = {10}, pages = {982-1017}, abstract = {Plants and associated microorganisms are used to remediate anthropogenic metal(loid) contamination of water, soils and sediments. this review focuses on the potential of arundo donax l. (giant reed) for alleviating risks due to soils, water, and sediments contaminated by trace elements (te), with emphasis on its advantages and limits over macrophytes and perennial grasses used for bioenergy and plant-based feedstock. arundo donax is relevant to phytomanage te-contaminated matrices, notably in its native area, as it possesses characteristics of large biomass production even under nutrient and abiotic stresses, fast growth rate, te tolerance and accumulation mainly in belowground plant parts. cultivating a. donax on contaminated lands and in constructed wetlands can contribute to increase land availability and limit the food vs. plant-based feedstock controversy. to gain more tools for decision-taking and sustainable management, further researches on a. donax should focus on: interactions between roots, te exposure, and rhizosphere and endophytic microorganisms; biomass response to (a)biotic factors; sustainable agricultural practices on marginal and contaminated land; integration into local, efficient, energy and biomass conversion chains with concern to biomass quality and production; life-cycle assessment including contaminant behavior, as well as environmental, agricultural and socio-economic benefits and drawbacks}, note = {ACL}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {article} } Plants and associated microorganisms are used to remediate anthropogenic metal(loid) contamination of water, soils and sediments. this review focuses on the potential of arundo donax l. (giant reed) for alleviating risks due to soils, water, and sediments contaminated by trace elements (te), with emphasis on its advantages and limits over macrophytes and perennial grasses used for bioenergy and plant-based feedstock. arundo donax is relevant to phytomanage te-contaminated matrices, notably in its native area, as it possesses characteristics of large biomass production even under nutrient and abiotic stresses, fast growth rate, te tolerance and accumulation mainly in belowground plant parts. cultivating a. donax on contaminated lands and in constructed wetlands can contribute to increase land availability and limit the food vs. plant-based feedstock controversy. to gain more tools for decision-taking and sustainable management, further researches on a. donax should focus on: interactions between roots, te exposure, and rhizosphere and endophytic microorganisms; biomass response to (a)biotic factors; sustainable agricultural practices on marginal and contaminated land; integration into local, efficient, energy and biomass conversion chains with concern to biomass quality and production; life-cycle assessment including contaminant behavior, as well as environmental, agricultural and socio-economic benefits and drawbacks |
Auteurs : Nsanganwimana Florien; Pourrut Bertrand; Mench Michel; Douay Francis Suitability of Miscanthus species for managing inorganic and organic contaminated land and restoring ecosystem services. A review Journal of Environmental Management, 143, pp. 123-1341 @article{Nsanganwimana2014a, title = {Suitability of Miscanthus species for managing inorganic and organic contaminated land and restoring ecosystem services. A review}, author = {Florien Nsanganwimana and Bertrand Pourrut and Michel Mench and Francis Douay}, year = {2014}, date = {2014-01-01}, journal = {Journal of Environmental Management}, volume = {143}, pages = {123-134}, abstract = {The mitigation of potential health hazards and land scarcity due to land use change can be addressed by restoring functional and ecosystem services of contaminated land. physico-chemical remediation options are criticized as being costly and not providing environment-friendly solutions. the use of plants and associated microorganisms could be a sustainable, cost-effective option to reduce pollutant exposure. phytomanagement aims at using valuable non-food crops to alleviate environmental and health risks induced by pollutants, and at restoring ecosystem services. suitable plant species must be tolerant to contaminants, reduce their transfer into the food chain, and efficiently produce marketable biomass. based on miscanthus' capacity to sequestrate inorganic contaminants into the root system and to induce dissipation of persistent organic contaminants in soil, these plant species are favorable for phytostabilization and phytodegradation. among miscanthus species, the noninvasive hybrid miscanthus x giganteus, with a high lignocellulosic content, is a promising biomass crop for the bioeconomy, notably the biorefinery and bioenergy industries. planting this species on contaminated and marginal land is a promising option to avoid changes in arable land use to mitigate the food vs. biofuel controversy. key issues in promoting sustainable management of miscanthus sp. on contaminated land are: (a) crop suitability, integration, and sustainability in a region with a potential local market; (b) site suitability in relation to the species' requirements and potential, (c) biotic interactions in the landscape diversity; and (d) increase in shoot yields in line with various stressors (e.g., pollutants, drought, cold temperatures), and with minimal inputs. (c) 2014 elsevier ltd. all rights reserved.}, note = {ACLS}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {article} } The mitigation of potential health hazards and land scarcity due to land use change can be addressed by restoring functional and ecosystem services of contaminated land. physico-chemical remediation options are criticized as being costly and not providing environment-friendly solutions. the use of plants and associated microorganisms could be a sustainable, cost-effective option to reduce pollutant exposure. phytomanagement aims at using valuable non-food crops to alleviate environmental and health risks induced by pollutants, and at restoring ecosystem services. suitable plant species must be tolerant to contaminants, reduce their transfer into the food chain, and efficiently produce marketable biomass. based on miscanthus' capacity to sequestrate inorganic contaminants into the root system and to induce dissipation of persistent organic contaminants in soil, these plant species are favorable for phytostabilization and phytodegradation. among miscanthus species, the noninvasive hybrid miscanthus x giganteus, with a high lignocellulosic content, is a promising biomass crop for the bioeconomy, notably the biorefinery and bioenergy industries. planting this species on contaminated and marginal land is a promising option to avoid changes in arable land use to mitigate the food vs. biofuel controversy. key issues in promoting sustainable management of miscanthus sp. on contaminated land are: (a) crop suitability, integration, and sustainability in a region with a potential local market; (b) site suitability in relation to the species' requirements and potential, (c) biotic interactions in the landscape diversity; and (d) increase in shoot yields in line with various stressors (e.g., pollutants, drought, cold temperatures), and with minimal inputs. (c) 2014 elsevier ltd. all rights reserved. |
Auteurs : Nsanganwimana Florien; Pourrut Bertrand; Waterlot Christophe; Bidar Géraldine; Douay Francis Intérêt de Miscanthus × giganteus pour le phytomanagement de sols agricoles contaminés par des éléments traces métalliques dans le Nord de la France 3èmes Rencontres nationales de la Recherche sur les sites et sols pollués, ADEME, 18-19 novembre 2014, Paris @conference{Nsanganwimana2014bb, title = {Intérêt de Miscanthus × giganteus pour le phytomanagement de sols agricoles contaminés par des éléments traces métalliques dans le Nord de la France}, author = {Florien Nsanganwimana and Bertrand Pourrut and Christophe Waterlot and Géraldine Bidar and Francis Douay}, year = {2014}, date = {2014-01-01}, booktitle = {3èmes Rencontres nationales de la Recherche sur les sites et sols pollués, ADEME, 18-19 novembre 2014, Paris}, note = {ACTN}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } |
Auteurs : Nsanganwimana Florien; Pourrut Bertrand; Waterlot Christophe; Douay Francis Miscanthus x giganteus: une graminée pérenne pour la gestion de sols agricoles contaminés par les éléments trace métalliques dans le nord de la France Journée de l'environnement, LaSalle Beauvais, 27 mars 2014, Beauvais @conference{Nsanganwimana2014c, title = {Miscanthus x giganteus: une graminée pérenne pour la gestion de sols agricoles contaminés par les éléments trace métalliques dans le nord de la France}, author = {Florien Nsanganwimana and Bertrand Pourrut and Christophe Waterlot and Francis Douay}, year = {2014}, date = {2014-01-01}, booktitle = {Journée de l'environnement, LaSalle Beauvais, 27 mars 2014, Beauvais}, note = {COM}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } |
Auteurs : Nsanganwimana Florien; Waterlot Christophe; Bidar Géraldine; Pourrut Bertrand; Douay Francis Comportement de Miscanthus cultivé sur des sols fortement contaminés en Cd, Pb et Zn Gestion et requalification durable des sites et sols pollués : Expériences en Nord - Pas de Calais, 25-26 septembre 2014, Villeneuve d’Ascq @conference{Nsanganwimana2014d, title = {Comportement de Miscanthus cultivé sur des sols fortement contaminés en Cd, Pb et Zn}, author = {Florien Nsanganwimana and Christophe Waterlot and Géraldine Bidar and Bertrand Pourrut and Francis Douay}, year = {2014}, date = {2014-01-01}, booktitle = {Gestion et requalification durable des sites et sols pollués : Expériences en Nord - Pas de Calais, 25-26 septembre 2014, Villeneuve d’Ascq}, abstract = {Objectifs spécifiques de l’étude §evaluer l’aptitude de m. × giganteus à se developer sur les sols contaminés §etudier l’influence de la culture sur les paramètres physico-chimiques des sols et le comportement de cd, pb et zn §déterminer l’accumulation des etm dans les organes de m. × giganteus selon le gradient de contamination des sols et les pratiques culturales}, note = {COM}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } Objectifs spécifiques de l’étude §evaluer l’aptitude de m. × giganteus à se developer sur les sols contaminés §etudier l’influence de la culture sur les paramètres physico-chimiques des sols et le comportement de cd, pb et zn §déterminer l’accumulation des etm dans les organes de m. × giganteus selon le gradient de contamination des sols et les pratiques culturales |
Auteurs : Pelfrêne Aurélie; Waterlot Christophe; Douay Francis Evaluation de l'impact de deux filières de phytomanagement sur la bioaccessibilité orale de Cd et Pb pour l’homme 3èmes Rencontres nationales de la Recherche sur les sites et sols pollués, ADEME, 18-19 novembre 2014, Paris @conference{Pelfrene2014, title = {Evaluation de l'impact de deux filières de phytomanagement sur la bioaccessibilité orale de Cd et Pb pour l’homme}, author = {Aurélie Pelfrêne and Christophe Waterlot and Francis Douay}, year = {2014}, date = {2014-01-01}, booktitle = {3èmes Rencontres nationales de la Recherche sur les sites et sols pollués, ADEME, 18-19 novembre 2014, Paris}, abstract = {Dans une démarche de gestion des sites et sols pollués, la requalification des sols par le phytomanagement permet de limiter les risques environnementaux et de maintenir une activité économique. le mode de gestion proposé dans la présente étude implique deux filières de phytomanagement à l’aide (1) d’une graminée vivace (miscanthus x giganteus) et (2) d’essences arborées avec et sans ajout d’amendements minéraux. en vue de mieux évaluer les dangers sanitaires en lien avec l’ingestion de particules de sols contaminés, la bioaccessibilité orale de cd et pb a été déterminée sur des sols collectés avant et après leur requalification. l’intérêt d’introduire la notion de bioaccessibilité au sein de l’évaluation de risques, au lieu des concentrations totales en métaux, est d’obtenir une valeur d’exposition plus pertinente. globalement, les modes de gestion proposés ne permettent pas de diminuer la bioaccessibilité orale de cd et pb dans la phase gastrique. dans la phase gastro-intestinale, qui d’un point de vue physiologique correspond à la phase la plus pertinente, la bioaccessibilité de cd n’est pas diminuée. en revanche, le phytomanagement (principalement avec le miscanthus) permet la réduction de la bioaccessibilité orale pour l’homme de pb.}, note = {ACTN}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } Dans une démarche de gestion des sites et sols pollués, la requalification des sols par le phytomanagement permet de limiter les risques environnementaux et de maintenir une activité économique. le mode de gestion proposé dans la présente étude implique deux filières de phytomanagement à l’aide (1) d’une graminée vivace (miscanthus x giganteus) et (2) d’essences arborées avec et sans ajout d’amendements minéraux. en vue de mieux évaluer les dangers sanitaires en lien avec l’ingestion de particules de sols contaminés, la bioaccessibilité orale de cd et pb a été déterminée sur des sols collectés avant et après leur requalification. l’intérêt d’introduire la notion de bioaccessibilité au sein de l’évaluation de risques, au lieu des concentrations totales en métaux, est d’obtenir une valeur d’exposition plus pertinente. globalement, les modes de gestion proposés ne permettent pas de diminuer la bioaccessibilité orale de cd et pb dans la phase gastrique. dans la phase gastro-intestinale, qui d’un point de vue physiologique correspond à la phase la plus pertinente, la bioaccessibilité de cd n’est pas diminuée. en revanche, le phytomanagement (principalement avec le miscanthus) permet la réduction de la bioaccessibilité orale pour l’homme de pb. |
Auteurs : Pelfrêne Aurélie; Waterlot Christophe; Proix Nicolas; Guérin Annie; Richard Antoine; Douay Francis Use of an in vitro digestion method to estimate cadmium bioaccessibility in vegetables grown in smelter-impacted soils: influence of cooking 30th International SEGH conference, 30 June – 4 July 2014, Newcastle upon Tyne (UK) @conference{Pelfrene2014a, title = {Use of an in vitro digestion method to estimate cadmium bioaccessibility in vegetables grown in smelter-impacted soils: influence of cooking}, author = {Aurélie Pelfrêne and Christophe Waterlot and Nicolas Proix and Annie Guérin and Antoine Richard and Francis Douay}, year = {2014}, date = {2014-01-01}, booktitle = {30th International SEGH conference, 30 June – 4 July 2014, Newcastle upon Tyne (UK)}, note = {ACTI}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } |
Auteurs : Pourrut Bertrand; Nsanganwimana Florien; Waterlot Christophe; Douay Francis Miscanthus x giganteus: a promising perennial grass for sustainable phytomanagement of heavy metal contaminated sites in Northern France Sustainable Remediation Conference 2014, 17-19 September 2014, Ferrara (Italie) @conference{Pourrut2014a, title = {Miscanthus x giganteus: a promising perennial grass for sustainable phytomanagement of heavy metal contaminated sites in Northern France}, author = {Bertrand Pourrut and Florien Nsanganwimana and Christophe Waterlot and Francis Douay}, year = {2014}, date = {2014-01-01}, booktitle = {Sustainable Remediation Conference 2014, 17-19 September 2014, Ferrara (Italie)}, abstract = {Soil contamination by metals is of major concern in northern france, particularly in the former coal-mining region. metaleurop nord, located at noyelles-godault, was a major european lead (pb) and zinc (zn) smelter for almost a century until its closure in 2003. in this area, the mean concentrations of cd, pb and zn in ploughed layers are 20-50 times higher than the regional background values and their concentrations in agricultural crops exceed threshold values for human consumption. contaminated dusts are also a risk for human health, especially children. the remediation of the highly contaminated soils is a major preoccupation for national and local authorities. however, the contaminated area is too large to be remediated in an economically relevant way by the currently applied remediation techniques. moreover, in order to find new income sources for local farmers, a sustainable management of these polluted soils is crucial. in this context, the phytener project aims to assess phytostabilization in combination with energy crop production (wood and miscanthus crops). the phytener consortium is a strong region-wide network of 11 laboratories, which leads a multidisciplinary approach combining a study of the impacts of these crops on soils and the environment, on social perceptions and on economic outcomes. in spring 2007, three approximately-1 hectare miscanthus giganteus experimental fields were established on former agricultural lands presenting a contamination gradient. the aim of this study was to (a) evaluate the ability of this plant to grow on metal-contaminated soils, b) to establish metal accumulation patterns in m. giganteus organs and c) to study the relationship between metal accumulation in the plant organs and their fractionations in soils. topsoil and plant samplings were carried out in september 2011. in field experimental conditions, the results show that m. giganteus grows well on highly contaminated soils. measured metal concentrations, bioconcentration and transfer factors demonstrate that this species accumulates metals mainly in roots and strongly limits their transfer to aboveground parts. in roots and rhizomes, the accumulation was as follows: cd > zn > pb. conversely, in stems and leaves it was zn ≥ cd > pb. though soils present a clear contamination! gradient, there were no significant difference in metal concentrations in stems and leaves. therefore, m. giganteus could be considered as a metal-excluder plant and appears to be a potential candidate crop for coupling phytostabilization and production of a valuable biomass on contaminated sites.}, note = {ACTI}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } Soil contamination by metals is of major concern in northern france, particularly in the former coal-mining region. metaleurop nord, located at noyelles-godault, was a major european lead (pb) and zinc (zn) smelter for almost a century until its closure in 2003. in this area, the mean concentrations of cd, pb and zn in ploughed layers are 20-50 times higher than the regional background values and their concentrations in agricultural crops exceed threshold values for human consumption. contaminated dusts are also a risk for human health, especially children. the remediation of the highly contaminated soils is a major preoccupation for national and local authorities. however, the contaminated area is too large to be remediated in an economically relevant way by the currently applied remediation techniques. moreover, in order to find new income sources for local farmers, a sustainable management of these polluted soils is crucial. in this context, the phytener project aims to assess phytostabilization in combination with energy crop production (wood and miscanthus crops). the phytener consortium is a strong region-wide network of 11 laboratories, which leads a multidisciplinary approach combining a study of the impacts of these crops on soils and the environment, on social perceptions and on economic outcomes. in spring 2007, three approximately-1 hectare miscanthus giganteus experimental fields were established on former agricultural lands presenting a contamination gradient. the aim of this study was to (a) evaluate the ability of this plant to grow on metal-contaminated soils, b) to establish metal accumulation patterns in m. giganteus organs and c) to study the relationship between metal accumulation in the plant organs and their fractionations in soils. topsoil and plant samplings were carried out in september 2011. in field experimental conditions, the results show that m. giganteus grows well on highly contaminated soils. measured metal concentrations, bioconcentration and transfer factors demonstrate that this species accumulates metals mainly in roots and strongly limits their transfer to aboveground parts. in roots and rhizomes, the accumulation was as follows: cd > zn > pb. conversely, in stems and leaves it was zn ≥ cd > pb. though soils present a clear contamination! gradient, there were no significant difference in metal concentrations in stems and leaves. therefore, m. giganteus could be considered as a metal-excluder plant and appears to be a potential candidate crop for coupling phytostabilization and production of a valuable biomass on contaminated sites. |
Auteurs : Poussin M; Pernin Céline; Leclercq Julie; Santorufo Lucia; Richard F J; Demuynck Sylvain; Douay Francis; Leprêtre Alain; Souty-Grosset Catherine Woodlice and recolonization of disturbed habitats in the Région Nord-Pas-de-Calais (France) 9th International Symposium of Terrestrial Isopod Biology, 26-30 juin 2014, Poitiers @conference{Poussin2014, title = {Woodlice and recolonization of disturbed habitats in the Région Nord-Pas-de-Calais (France)}, author = {M Poussin and Céline Pernin and Julie Leclercq and Lucia Santorufo and F J Richard and Sylvain Demuynck and Francis Douay and Alain Leprêtre and Catherine Souty-Grosset}, year = {2014}, date = {2014-01-01}, booktitle = {9th International Symposium of Terrestrial Isopod Biology, 26-30 juin 2014, Poitiers}, note = {ACTI}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } |
Auteurs : Santorufo Lucia; Leclercq Julie; Louvel Brice; Douay Francis Linking litter characteristics and microbial communities in different stages of decomposition in fly ash amended soils First Global Soil Biodiversity Conference - Assessing soil biodiversity and its role for ecosystem services, 2-5 décembre 2014, Dijon @conference{Santorufo2014, title = {Linking litter characteristics and microbial communities in different stages of decomposition in fly ash amended soils}, author = {Lucia Santorufo and Julie Leclercq and Brice Louvel and Francis Douay}, year = {2014}, date = {2014-01-01}, booktitle = {First Global Soil Biodiversity Conference - Assessing soil biodiversity and its role for ecosystem services, 2-5 décembre 2014, Dijon}, note = {ACTI}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } |
Auteurs : Verdin Anthony; Fontaine Joël; Firmin Stéphane; Labidi Sonia; Bidar Géraldine; Lopareva-Pohu Alena; Laruelle Frédéric; Pourrut Bertrand; Douay Francis; Lounès-Hadj-Sahraoui Anissa; Shirali Pirouz Phytostabilisation assistée: Influence des amendements minéraux sur le comportement des ETM et la viabilité de la microflore tellurique, des strates herbacée et arborée sur un sol historiquement pollué 3èmes Rencontres nationales de la Recherche sur les sites et sols pollués, ADEME, 18-19 novembre 2014, Paris @conference{Verdin2014, title = {Phytostabilisation assistée: Influence des amendements minéraux sur le comportement des ETM et la viabilité de la microflore tellurique, des strates herbacée et arborée sur un sol historiquement pollué}, author = {Anthony Verdin and Joël Fontaine and Stéphane Firmin and Sonia Labidi and Géraldine Bidar and Alena Lopareva-Pohu and Frédéric Laruelle and Bertrand Pourrut and Francis Douay and Anissa Lounès-Hadj-Sahraoui and Pirouz Shirali}, year = {2014}, date = {2014-01-01}, booktitle = {3èmes Rencontres nationales de la Recherche sur les sites et sols pollués, ADEME, 18-19 novembre 2014, Paris}, note = {ACTN}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } |
Auteurs : Waterlot Christophe; Douay Francis; Daurangeon Fabien Intérêt de la méthode de correction du bruit de fond par renversement de spectre lors du dosage par spectrométrie d’absorption atomique électrothermique de l’arsenic dans des matrices contenant des concentrations élevées en aluminium Spectra Analyse, 297, pp. 52-561 @article{Waterlot2014, title = {Intérêt de la méthode de correction du bruit de fond par renversement de spectre lors du dosage par spectrométrie d’absorption atomique électrothermique de l’arsenic dans des matrices contenant des concentrations élevées en aluminium}, author = {Christophe Waterlot and Francis Douay and Fabien Daurangeon}, year = {2014}, date = {2014-01-01}, journal = {Spectra Analyse}, volume = {297}, pages = {52-56}, note = {ACLN}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {article} } |
Auteurs : Waterlot Christophe; Douay Francis; Daurangeon Fabien Réduction des effets de matrices et des interférences lors du dosage de l’antimoine par spectrométrie d’absorption atomique électrothermique Spectra Analyse, 297, pp. 58-631 @article{Waterlot2014a, title = {Réduction des effets de matrices et des interférences lors du dosage de l’antimoine par spectrométrie d’absorption atomique électrothermique}, author = {Christophe Waterlot and Francis Douay and Fabien Daurangeon}, year = {2014}, date = {2014-01-01}, journal = {Spectra Analyse}, volume = {297}, pages = {58-63}, note = {ACLN}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {article} } |
Auteurs : Waterlot Christophe; Pelfrêne Aurélie; Nsanganwimana Florien; Pourrut Bertrand; Douay Francis Évaluation des effets de Miscanthus × giganteus sur la distribution des ETM d’une parcelle agricole 3èmes Rencontres nationales de la Recherche sur les sites et sols pollués, ADEME, 18-19 novembre 2014, Paris @conference{Waterlot2014b, title = {Évaluation des effets de Miscanthus × giganteus sur la distribution des ETM d’une parcelle agricole}, author = {Christophe Waterlot and Aurélie Pelfrêne and Florien Nsanganwimana and Bertrand Pourrut and Francis Douay}, year = {2014}, date = {2014-01-01}, booktitle = {3èmes Rencontres nationales de la Recherche sur les sites et sols pollués, ADEME, 18-19 novembre 2014, Paris}, abstract = {Le phytomanagement peut être un mode de gestion de sols contaminés en visant à limiter les dangers environnementaux et sanitaires mais aussi, à requalifier les milieux dégradés en restaurant leurs fonctions. l’un des modes de gestion proposé dans le cadre du programme phytener repose sur l’utilisation, à des fins de production d’une biomasse, d’une graminée vivace et pérenne, miscanthus × giganteus. dans le but d’étudier les effets du couvert végétal sur la mobilité des métaux, des extractions séquentielles ont été réalisées avant et après son installation. bien que la mobilité de cd, pb et zn ait significativement diminué la première année qui a suivi la mise en place du dispositif expérimental, il s’avère que l’extractabilité de cd et pb reste globalement inchangée à l’issue de trois années d’expérimentation. en revanche, miscanthus × giganteus induit une augmentation de la mobilité de zn.}, note = {ACTN}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } Le phytomanagement peut être un mode de gestion de sols contaminés en visant à limiter les dangers environnementaux et sanitaires mais aussi, à requalifier les milieux dégradés en restaurant leurs fonctions. l’un des modes de gestion proposé dans le cadre du programme phytener repose sur l’utilisation, à des fins de production d’une biomasse, d’une graminée vivace et pérenne, miscanthus × giganteus. dans le but d’étudier les effets du couvert végétal sur la mobilité des métaux, des extractions séquentielles ont été réalisées avant et après son installation. bien que la mobilité de cd, pb et zn ait significativement diminué la première année qui a suivi la mise en place du dispositif expérimental, il s’avère que l’extractabilité de cd et pb reste globalement inchangée à l’issue de trois années d’expérimentation. en revanche, miscanthus × giganteus induit une augmentation de la mobilité de zn. |
Auteurs : Leclercq Julie; Santorufo Lucia; Pernin Céline; Demuynck Sylvain; Louvel Brice; Grumiaux Fabien; Pruvot Christelle; Douay Francis; Leprêtre Alain Évaluation de méthodes d’ingénierie écologique visant à restaurer les habitats dégradés: Intérêt du mulching dans la reconnexion écologique Gestion et requalification durable des sites et sols pollués : Expériences en Nord - Pas de Calais, 25-26 septembre 2014, Villeneuve d’Ascq @conference{Leclercq2014d, title = {Évaluation de méthodes d’ingénierie écologique visant à restaurer les habitats dégradés: Intérêt du mulching dans la reconnexion écologique}, author = {Julie Leclercq and Lucia Santorufo and Céline Pernin and Sylvain Demuynck and Brice Louvel and Fabien Grumiaux and Christelle Pruvot and Francis Douay and Alain Leprêtre}, year = {2014}, date = {2014-01-01}, booktitle = {Gestion et requalification durable des sites et sols pollués : Expériences en Nord - Pas de Calais, 25-26 septembre 2014, Villeneuve d’Ascq}, note = {COM}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } |
Auteurs : Burrow Coralie; Détriché Sébastien; Douay Francis; Leclercq Julie; Leprêtre Alain; Pernin Céline; Masson Eric Apport de l’imagerie pour la validation de campagne d’échantillonnage "Du payasage Image à l’Image Paysage. Concepts & méthodes appliqués à l’analyse participative des paysage ", Séminaire ADA, MESHS Lille Nord de France, 18-19 novembre 2013, Lille @conference{Burrow2013, title = {Apport de l’imagerie pour la validation de campagne d’échantillonnage}, author = {Coralie Burrow and Sébastien Détriché and Francis Douay and Julie Leclercq and Alain Leprêtre and Céline Pernin and Eric Masson}, year = {2013}, date = {2013-01-01}, booktitle = {"Du payasage Image à l’Image Paysage. Concepts & méthodes appliqués à l’analyse participative des paysage ", Séminaire ADA, MESHS Lille Nord de France, 18-19 novembre 2013, Lille}, note = {COM}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } |
Auteurs : Chenot Elodie-Denise; Douay Francis; Dumat Camille; Pernin Céline; Pourrut Bertrand; Schwartz Christophe Jardins potagers: terres inconnues ? @book{Chenot2013, title = {Jardins potagers: terres inconnues ?}, author = {Elodie-Denise Chenot and Francis Douay and Camille Dumat and Céline Pernin and Bertrand Pourrut and Christophe Schwartz}, year = {2013}, date = {2013-01-01}, volume = {Eds EDP, 172pp}, note = {OS}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {book} } |
Auteurs : Demuynck Sylvain; Succiu I -R; Pernin Céline; Douay Francis; Leprêtre Alain The Eisenia fetida avoidance behaviour test to assess metal-contaminated soil quality recovery after phytostabilisation SETAC Europe 23rd Annual Meeting, 12-16 May 2013, Glasgow, Scotland (UK) @conference{Demuynck2013, title = {The Eisenia fetida avoidance behaviour test to assess metal-contaminated soil quality recovery after phytostabilisation}, author = {Sylvain Demuynck and I -R Succiu and Céline Pernin and Francis Douay and Alain Leprêtre}, year = {2013}, date = {2013-01-01}, booktitle = {SETAC Europe 23rd Annual Meeting, 12-16 May 2013, Glasgow, Scotland (UK)}, note = {ACTI}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } |
Auteurs : Douay Francis Sols et environnement Colloque Paysage Transfrontalier; Cœur du Hainaut 2025: Ecriture des paysages d’une regénération industrielle; Faculté d’Architecture et d’Urbanisme de l’Université de Mons, 27-28 mars 2013, Mons (Belgique) @conference{Douay2013a, title = {Sols et environnement}, author = {Francis Douay}, year = {2013}, date = {2013-01-01}, booktitle = {Colloque Paysage Transfrontalier; Cœur du Hainaut 2025: Ecriture des paysages d’une regénération industrielle; Faculté d’Architecture et d’Urbanisme de l’Université de Mons, 27-28 mars 2013, Mons (Belgique)}, note = {COM}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } |
Auteurs : Douay Francis; Nsanganwimana Florien; Bidar Géraldine; Pourrut Bertrand Apport du phytomanagement dans la gestion de territoires fortement dégradés par les activités humaines : Exemple de Metaleurop (France) Colloque Paysage Transfrontalier; Cœur du Hainaut 2025: Ecriture des paysages d’une regénération industrielle; Faculté d’Architecture et d’Urbanisme de l’Université de Mons, 27-28 mars 2013, Mons (Belgique) @conference{Douay2013b, title = {Apport du phytomanagement dans la gestion de territoires fortement dégradés par les activités humaines : Exemple de Metaleurop (France)}, author = {Francis Douay and Florien Nsanganwimana and Géraldine Bidar and Bertrand Pourrut}, year = {2013}, date = {2013-01-01}, booktitle = {Colloque Paysage Transfrontalier; Cœur du Hainaut 2025: Ecriture des paysages d’une regénération industrielle; Faculté d’Architecture et d’Urbanisme de l’Université de Mons, 27-28 mars 2013, Mons (Belgique)}, note = {COM}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } |
Auteurs : Douay Francis; Pelfrêne Aurélie; Planque Julie; Fourrier Hervé; Richard Antoine; Roussel Hélène; Girondelot Bertrand Assessment of potential health risk for inhabitants living near a former lead smelter. Part 1: metal concentrations in soils, agricultural crops, and homegrown vegetables Environmental Monitoring and Assessment, 185(5), pp. 3665-36801 @article{Douay2013c, title = {Assessment of potential health risk for inhabitants living near a former lead smelter. Part 1: metal concentrations in soils, agricultural crops, and homegrown vegetables}, author = {Francis Douay and Aurélie Pelfrêne and Julie Planque and Hervé Fourrier and Antoine Richard and Hélène Roussel and Bertrand Girondelot}, year = {2013}, date = {2013-01-01}, journal = {Environmental Monitoring and Assessment}, volume = {185}, number = {5}, pages = {3665-3680}, abstract = {Soil contamination by metals engenders important environmental and health problems in northern france where a smelter (metaleurop nord) was in activity for more than a century. this study aims to look at the long-term effects of the smelter after its closedown by combining data on the degree of soil contamination and the quality of the crops grown (agricultural crops and homegrown vegetables) in these soils for a better assessment of the local population's exposure to cd, pb, and zn. seven years after the metaleurop nord closedown, (1) the agricultural and urban topsoils were strongly contaminated by cd, pb, and zn; (2) the kitchen garden topsoils were even more polluted than the agricultural soils, with great variability in metal concentrations within the gardens studied; (3) a high proportion of the agricultural crops for foodstuffs did not conform with the european legislation; (4) for feedstuffs, most samples did not exceed the cd and pb legislation limits, indicating that feedstuffs may be an opportunity for most agricultural produce; and (5) a high proportion of the vegetables produced in the kitchen gardens did not conform with the european foodstuff legislation. the high contamination level of the soils studied continues to be a risk for the environment and the population's health. a further investigation (part 2) assesses the associated potential health risk for local inhabitants through consumption of homegrown vegetables and ingestion of soil particles by estimating the site-specific human health assessment criteria for cd and pb.}, note = {ACL}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {article} } Soil contamination by metals engenders important environmental and health problems in northern france where a smelter (metaleurop nord) was in activity for more than a century. this study aims to look at the long-term effects of the smelter after its closedown by combining data on the degree of soil contamination and the quality of the crops grown (agricultural crops and homegrown vegetables) in these soils for a better assessment of the local population's exposure to cd, pb, and zn. seven years after the metaleurop nord closedown, (1) the agricultural and urban topsoils were strongly contaminated by cd, pb, and zn; (2) the kitchen garden topsoils were even more polluted than the agricultural soils, with great variability in metal concentrations within the gardens studied; (3) a high proportion of the agricultural crops for foodstuffs did not conform with the european legislation; (4) for feedstuffs, most samples did not exceed the cd and pb legislation limits, indicating that feedstuffs may be an opportunity for most agricultural produce; and (5) a high proportion of the vegetables produced in the kitchen gardens did not conform with the european foodstuff legislation. the high contamination level of the soils studied continues to be a risk for the environment and the population's health. a further investigation (part 2) assesses the associated potential health risk for local inhabitants through consumption of homegrown vegetables and ingestion of soil particles by estimating the site-specific human health assessment criteria for cd and pb. |
Auteurs : Douay Francis; Pourrut Bertrand; Bidar Géraldine; Pruvot Christelle La gestion des sols dégradés par les activités humaines: l'exemple de Metaleurop Au Pays des Racines, Journée de conférences et d’échanges Société Nationale d’Horticulture de France, 31 janvier 2013, Lomme @conference{Douay2013cb, title = {La gestion des sols dégradés par les activités humaines: l'exemple de Metaleurop}, author = {Francis Douay and Bertrand Pourrut and Géraldine Bidar and Christelle Pruvot}, year = {2013}, date = {2013-01-01}, booktitle = {Au Pays des Racines, Journée de conférences et d’échanges Société Nationale d’Horticulture de France, 31 janvier 2013, Lomme}, note = {OV}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } |
Auteurs : Firmin Stéphane; Labidi Sonia; Verdin Anthony; Lounès-Hadj-Sahraoui Anissa; Fontaine Joël; Billet S; Pourrut Bertrand; Douay Francis; Shirali Pirouz Influence of fly ash aided phytostabilisation of Pb, Cd and Zn highly contaminated site on tree species oxidative stress Urban Environmental Pollution 2013, 17-20 November 2013, Beijing (China) @conference{Firmin2013, title = {Influence of fly ash aided phytostabilisation of Pb, Cd and Zn highly contaminated site on tree species oxidative stress}, author = {Stéphane Firmin and Sonia Labidi and Anthony Verdin and Anissa Lounès-Hadj-Sahraoui and Joël Fontaine and S Billet and Bertrand Pourrut and Francis Douay and Pirouz Shirali}, year = {2013}, date = {2013-01-01}, booktitle = {Urban Environmental Pollution 2013, 17-20 November 2013, Beijing (China)}, note = {ACTI}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } |
Auteurs : Fourrier Hervé; Détriché Sébastien; Douay Francis Les sols en Nord-Pas de Calais Au Pays des Racines; Journée de conférences et d’échanges Société Nationale d’Horticulture de France, 31 janvier 2013, Lomme @conference{Fourrier2013, title = {Les sols en Nord-Pas de Calais}, author = {Hervé Fourrier and Sébastien Détriché and Francis Douay}, year = {2013}, date = {2013-01-01}, booktitle = {Au Pays des Racines; Journée de conférences et d’échanges Société Nationale d’Horticulture de France, 31 janvier 2013, Lomme}, note = {OV}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } |
Auteurs : Goulas Anaïs; Pelfrêne Aurélie; Douay Francis; Waterlot Christophe Optimisation des conditions d’analyses des HAP de la liste US EPA en UFLC munie d’un détecteur à barrettes de diodes et d’une colonne Kinetex®- C18 à particules Core-Shell Spectra Analyse, 295, pp. 48-531 @article{Goulas2013, title = {Optimisation des conditions d’analyses des HAP de la liste US EPA en UFLC munie d’un détecteur à barrettes de diodes et d’une colonne Kinetex®- C18 à particules Core-Shell}, author = {Anaïs Goulas and Aurélie Pelfrêne and Francis Douay and Christophe Waterlot}, year = {2013}, date = {2013-01-01}, journal = {Spectra Analyse}, volume = {295}, pages = {48-53}, abstract = {Les hydrocarbures aromatiques polycycliques (hap) sont présents dans les compartiments environnementaux (sols, sédiments, particules en suspension dans l’eau et dans l’air) et dans la chaîne alimentaire. connus pour leurs effets mutagènes, génotoxiques et cancérogènes, les hap font l’objet de nombreuses études portant sur la caractérisation des dangers qu’ils représentent, soit individuellement, soit en mélange. quelles que soient les matrices contaminées, la quantification à l’état de traces et d’ultratraces des hap nécessite des techniques analytiques permettant une séparation des composés avec une sensibilité optimale. les caractéristiques analytiques d’un système associant une chaîne uflc (ultra fast liquid chromatography), un détecteur à barrettes de diodes et une colonne équipée des particules core- shell ont donc été déterminées pour l’analyse des 16 hap classés prioritaires par l’agence de protection de l’environnement américaine (us epa). leur séparation a été réalisée en 21,5 minutes et un bon compromis entre ce temps et la résolution des pics a permis l’intégration automatisée de l’ensemble des hap au point d’obtenir des limites de détection faibles (comprises entre 0,88 et 9,16 μg.l-1) au regard du détecteur utilisé.}, note = {ACLN}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {article} } Les hydrocarbures aromatiques polycycliques (hap) sont présents dans les compartiments environnementaux (sols, sédiments, particules en suspension dans l’eau et dans l’air) et dans la chaîne alimentaire. connus pour leurs effets mutagènes, génotoxiques et cancérogènes, les hap font l’objet de nombreuses études portant sur la caractérisation des dangers qu’ils représentent, soit individuellement, soit en mélange. quelles que soient les matrices contaminées, la quantification à l’état de traces et d’ultratraces des hap nécessite des techniques analytiques permettant une séparation des composés avec une sensibilité optimale. les caractéristiques analytiques d’un système associant une chaîne uflc (ultra fast liquid chromatography), un détecteur à barrettes de diodes et une colonne équipée des particules core- shell ont donc été déterminées pour l’analyse des 16 hap classés prioritaires par l’agence de protection de l’environnement américaine (us epa). leur séparation a été réalisée en 21,5 minutes et un bon compromis entre ce temps et la résolution des pics a permis l’intégration automatisée de l’ensemble des hap au point d’obtenir des limites de détection faibles (comprises entre 0,88 et 9,16 μg.l-1) au regard du détecteur utilisé. |
Auteurs : Goulas Anaïs; Pelfrêne Aurélie; Douay Francis; Waterlot Christophe Optimisation des conditions d’analyses des HAP en UFLC équipée d’un détecteur à barrettes de diodes et d’une colonne Kinetex®- C18 Université Catholique de Lille - Journée de la R&D en développement durable et responsabilité sociétale, 31 mai 2013, Lille @conference{Goulas2013a, title = {Optimisation des conditions d’analyses des HAP en UFLC équipée d’un détecteur à barrettes de diodes et d’une colonne Kinetex®- C18}, author = {Anaïs Goulas and Aurélie Pelfrêne and Francis Douay and Christophe Waterlot}, year = {2013}, date = {2013-01-01}, booktitle = {Université Catholique de Lille - Journée de la R&D en développement durable et responsabilité sociétale, 31 mai 2013, Lille}, note = {COM}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } |
Auteurs : Hayet A; de-Foucault Bruno; Douay Francis; Deram Annabelle Impact du Miscanthus sur la biodiversité végétale de sols contaminés par des éléments traces métalliques Etude et Gestion des Sols, 20(2), pp. 151-1611 @article{Hayet2013, title = {Impact du Miscanthus sur la biodiversité végétale de sols contaminés par des éléments traces métalliques}, author = {A Hayet and Bruno de-Foucault and Francis Douay and Annabelle Deram}, year = {2013}, date = {2013-01-01}, journal = {Etude et Gestion des Sols}, volume = {20}, number = {2}, pages = {151-161}, abstract = {Dans le cadre du programme phytener, une évaluation de l'influence de la production de miscanthus giganteus sur la biodiversité végétale de parcelles cultivées a été réalisée afin d'apprécier la viabilité écologique de ce type de culture sur sols contaminés. pour ce faire, la flore vasculaire de six parcelles expérimentales, toutes plantées en miscanthus, a été analysée. quatre de ces parcelles présentent des sols contaminés à des degrés divers par des éléments traces métalliques (etm). les concentrations en etm des deux autres parcelles avoisinent celles des teneurs agricoles habituelles (tah). ces parcelles ont été considérées comme des références dans la démarche. les résultats montrent que la flore adventice des cultures de miscanthus se compose essentiellement d'espèces indigènes, communes et peu menacées (niveau de préoccupation mineure). au niveau communautaire, deux grands types biologiques sont représentés : les annuelles (végétation thérophytique) et les vivaces. ces communautés d'adventices témoignent des perturbations occasionnées par la plantation de miscanthus et de l'hétérogénéité locale du milieu. sur la base des résultats obtenus, la production de miscanthus ne semble pas exercer d'influence néfaste sur les adventices de culture. en retour, les adventices ne semblent pas nuire au développement de miscanthus, une fois la période d'implantation réussie. dans notre contexte, la contamination des sols ne semble pas constituer un obstacle à la colonisation des cultures par les adventices. les facteurs influant sur la colonisation des cultures par les adventices sont étroitement liés au contexte environnant et à la durée du cycle des cultures. en effet, une hétérogénéité spatiale des paysages à proximité des parcelles permet une meilleure diversité des adventices et un cycle de culture long est favorable à une reprise de la dynamique évolutive de la végétation.}, note = {ACLN}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {article} } Dans le cadre du programme phytener, une évaluation de l'influence de la production de miscanthus giganteus sur la biodiversité végétale de parcelles cultivées a été réalisée afin d'apprécier la viabilité écologique de ce type de culture sur sols contaminés. pour ce faire, la flore vasculaire de six parcelles expérimentales, toutes plantées en miscanthus, a été analysée. quatre de ces parcelles présentent des sols contaminés à des degrés divers par des éléments traces métalliques (etm). les concentrations en etm des deux autres parcelles avoisinent celles des teneurs agricoles habituelles (tah). ces parcelles ont été considérées comme des références dans la démarche. les résultats montrent que la flore adventice des cultures de miscanthus se compose essentiellement d'espèces indigènes, communes et peu menacées (niveau de préoccupation mineure). au niveau communautaire, deux grands types biologiques sont représentés : les annuelles (végétation thérophytique) et les vivaces. ces communautés d'adventices témoignent des perturbations occasionnées par la plantation de miscanthus et de l'hétérogénéité locale du milieu. sur la base des résultats obtenus, la production de miscanthus ne semble pas exercer d'influence néfaste sur les adventices de culture. en retour, les adventices ne semblent pas nuire au développement de miscanthus, une fois la période d'implantation réussie. dans notre contexte, la contamination des sols ne semble pas constituer un obstacle à la colonisation des cultures par les adventices. les facteurs influant sur la colonisation des cultures par les adventices sont étroitement liés au contexte environnant et à la durée du cycle des cultures. en effet, une hétérogénéité spatiale des paysages à proximité des parcelles permet une meilleure diversité des adventices et un cycle de culture long est favorable à une reprise de la dynamique évolutive de la végétation. |
Auteurs : Leclercq Julie; Louvel Brice; Pruvot Christelle; Douay Francis Ecologie et restauration de la biodiversité des sols perturbés dans un contexte de phytomanagement Journée de la recherche de l’UCL. 21 mai 2013, Lille @conference{Leclercq2013, title = {Ecologie et restauration de la biodiversité des sols perturbés dans un contexte de phytomanagement}, author = {Julie Leclercq and Brice Louvel and Christelle Pruvot and Francis Douay}, year = {2013}, date = {2013-01-01}, booktitle = {Journée de la recherche de l’UCL. 21 mai 2013, Lille}, note = {COM}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } |
Auteurs : Nsanganwimana Florien; Pourrut Bertrand; Douay Francis Miscanthus giganteus: A perennial grass suitable for coupling biomass production and management of metal-contaminated sites in northern France. Sustainable approached for remediation of contaminated land in Europe (SARCLE-2013) Contaminated Site Management in Europe (CSME-2013). 22-24 October 2013, Amsterdam (Pays-Bas) @conference{Nsanganwimana2013, title = {Miscanthus giganteus: A perennial grass suitable for coupling biomass production and management of metal-contaminated sites in northern France. Sustainable approached for remediation of contaminated land in Europe (SARCLE-2013)}, author = {Florien Nsanganwimana and Bertrand Pourrut and Francis Douay}, year = {2013}, date = {2013-01-01}, booktitle = {Contaminated Site Management in Europe (CSME-2013). 22-24 October 2013, Amsterdam (Pays-Bas)}, note = {ACTI}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } |
Auteurs : Pauget Benjamin; Gimbert Frédéric; Coeurdassier Michaël; Crini Nadia; Pérès Guénola; Faure Olivier; Douay Francis; Hitmi Adnane; Béguiristain Thierry; Alaphilippe A; Guernion Muriel; Houot Sabine; Legras Marc; Vian Jean-François; Hedde Mickaël; Bispo Antonio; Grand Cécile; De-Vaufleury Annette Ranking field site management priorities according to their metal transfer to snails Ecological Indicators, 29, pp. 445-4541 @article{Pauget2013, title = {Ranking field site management priorities according to their metal transfer to snails}, author = {Benjamin Pauget and Frédéric Gimbert and Michaël Coeurdassier and Nadia Crini and Guénola Pérès and Olivier Faure and Francis Douay and Adnane Hitmi and Thierry Béguiristain and A Alaphilippe and Muriel Guernion and Sabine Houot and Marc Legras and Jean-François Vian and Mickaël Hedde and Antonio Bispo and Cécile Grand and Annette De-Vaufleury}, year = {2013}, date = {2013-01-01}, journal = {Ecological Indicators}, volume = {29}, pages = {445-454}, abstract = {Current soil quality evaluation does not include an assessment of metal bioavailability to organisms. however, sentinel soil-dwelling invertebrates can be used for such an assessment. this study aims to establish the modulating soil parameter of metal bioavailability to snails and a procedure for ranking field sites (n = 9; 43 plots) based on the evaluation of the transfer of metals to the land snails used as indicators of metal zooavailability. multivariate regressions identify soil ph, organic carbon and iron oxides influence cadmium, chromium, copper, lead and zinc zooavailability to snails underlining the need to consider other parameter than total soil concentration during bioavailability assessment. however, for as, no influence of soil parameter on it bioavailability to snails was identified. internal concentrations of reference (ciref) of cd, pb, as, cr, cu and zn were determined in cantareus aspersus that were caged on unpolluted plots. ciref allow for the identification of contaminated sites. ciref have revealed unexpected metal transfer on some “unpolluted” sites and a lack of transfer on some contaminated sites, thus confirming the necessity for biological measures to evaluate metal mobility. the sum of excess of transfers (set) index ranked the industrially impacted sites as the top priorities for management. we recommend that the set methodology be used for future environmental risk assessment. by highlighting real metal transfers and considering the numerous parameters influencing environmental bioavailability, the snails watch provides information on environmental quality.}, note = {ACL}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {article} } Current soil quality evaluation does not include an assessment of metal bioavailability to organisms. however, sentinel soil-dwelling invertebrates can be used for such an assessment. this study aims to establish the modulating soil parameter of metal bioavailability to snails and a procedure for ranking field sites (n = 9; 43 plots) based on the evaluation of the transfer of metals to the land snails used as indicators of metal zooavailability. multivariate regressions identify soil ph, organic carbon and iron oxides influence cadmium, chromium, copper, lead and zinc zooavailability to snails underlining the need to consider other parameter than total soil concentration during bioavailability assessment. however, for as, no influence of soil parameter on it bioavailability to snails was identified. internal concentrations of reference (ciref) of cd, pb, as, cr, cu and zn were determined in cantareus aspersus that were caged on unpolluted plots. ciref allow for the identification of contaminated sites. ciref have revealed unexpected metal transfer on some “unpolluted” sites and a lack of transfer on some contaminated sites, thus confirming the necessity for biological measures to evaluate metal mobility. the sum of excess of transfers (set) index ranked the industrially impacted sites as the top priorities for management. we recommend that the set methodology be used for future environmental risk assessment. by highlighting real metal transfers and considering the numerous parameters influencing environmental bioavailability, the snails watch provides information on environmental quality. |
Auteurs : Pauget Benjamin; Gimbert Frédéric; Coeurdassier Michaël; Crini Nadia; Pérès Guénola; Faure Olivier; Douay Francis; Richard Antoine; Grand Cécile; De-Vaufleury Annette Assessing the in situ bioavailability of trace elements to snails using accumulation kinetics Ecological Indicators, 34, pp. 126-1351 @article{Pauget2013a, title = {Assessing the in situ bioavailability of trace elements to snails using accumulation kinetics}, author = {Benjamin Pauget and Frédéric Gimbert and Michaël Coeurdassier and Nadia Crini and Guénola Pérès and Olivier Faure and Francis Douay and Antoine Richard and Cécile Grand and Annette De-Vaufleury}, year = {2013}, date = {2013-01-01}, journal = {Ecological Indicators}, volume = {34}, pages = {126-135}, abstract = {The bioavailability of trace elements in soils is conditioned by both physico-chemical and biological parameters. in this study, the accumulation kinetics of cadmium (cd), lead (pb), arsenic (as) and antimony (sb) were determined for 3 industrially impacted sites to assess the bioavailability of these contaminants to the garden snail (cantareus aspersus). mono and multivariate regressions allowed the identification of cation exchange capacity (cec), silts and organic carbon content as the soil parameters modulating the in situ bioavailability of cd and pb. for all elements, the total concentrations in the soils were not good predictor (not significant correlation) of the bioavailability to snails. the cd, as and sb assimilation fluxes were correlated with the calcium chloride (cacl2) extract concentrations, but this correlation was not observed with pb. the total soil concentration coupled with soil properties best explained the variation in pb assimilation, whereas their influences on cd bioavailability were lower, signifying that other parameters such as contamination sources may modulate cd bioavailability. here, the as and sb in situ accumulation kinetics are described for the first time and highlighted a slight bioavailability to snails at the studied sites. the absence of a correlation between the as or sb assimilation fluxes and total metals in the soil coupled with the absence of influence of soil properties on their bioavailability may result from the speciation of these metalloids, which are known to modulate their mobility in soils. this study highlights the need to consider both physico-chemical and biological aspects of metal and metalloid bioavailability to assess the risk of metal transfer from soil to organisms. (c) 2013 elsevier ltd. all rights reserved.}, note = {ACL}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {article} } The bioavailability of trace elements in soils is conditioned by both physico-chemical and biological parameters. in this study, the accumulation kinetics of cadmium (cd), lead (pb), arsenic (as) and antimony (sb) were determined for 3 industrially impacted sites to assess the bioavailability of these contaminants to the garden snail (cantareus aspersus). mono and multivariate regressions allowed the identification of cation exchange capacity (cec), silts and organic carbon content as the soil parameters modulating the in situ bioavailability of cd and pb. for all elements, the total concentrations in the soils were not good predictor (not significant correlation) of the bioavailability to snails. the cd, as and sb assimilation fluxes were correlated with the calcium chloride (cacl2) extract concentrations, but this correlation was not observed with pb. the total soil concentration coupled with soil properties best explained the variation in pb assimilation, whereas their influences on cd bioavailability were lower, signifying that other parameters such as contamination sources may modulate cd bioavailability. here, the as and sb in situ accumulation kinetics are described for the first time and highlighted a slight bioavailability to snails at the studied sites. the absence of a correlation between the as or sb assimilation fluxes and total metals in the soil coupled with the absence of influence of soil properties on their bioavailability may result from the speciation of these metalloids, which are known to modulate their mobility in soils. this study highlights the need to consider both physico-chemical and biological aspects of metal and metalloid bioavailability to assess the risk of metal transfer from soil to organisms. (c) 2013 elsevier ltd. all rights reserved. |
Auteurs : Pelfrêne Aurélie; Douay Francis; Richard Antoine; Roussel Hélène; Girondelot Bertrand Assessment of potential health risk for inhabitants living near a former lead smelter. Part 2: Site-specific human health risk assessment of Cd and Pb contamination in kitchen gardens Environmental Monitoring and Assessment, 185(5), pp. 2999-30121 @article{Pelfrene2013b, title = {Assessment of potential health risk for inhabitants living near a former lead smelter. Part 2: Site-specific human health risk assessment of Cd and Pb contamination in kitchen gardens}, author = {Aurélie Pelfrêne and Francis Douay and Antoine Richard and Hélène Roussel and Bertrand Girondelot}, year = {2013}, date = {2013-01-01}, journal = {Environmental Monitoring and Assessment}, volume = {185}, number = {5}, pages = {2999-3012}, abstract = {Metal contamination of urban soils and homegrown products has caused major concern. in part 1, we investigated the long-term effects of a former smelter on the degree of kitchen garden-soil contamination and the quality of the homegrown vegetables from these gardens. the results showed that the soils retained a high level of contamination and that a large proportion of the vegetables produced did not comply with the legislation on the levels of metals allowed for human consumption. the present study aims to assess the associated potential health risk to local inhabitants through consumption of homegrown vegetables and ingestion of soil particles using a land use-based approach. for lead (pb), the standard hazard quotient (hq)-based risk assessment method was used to determine the hq. for cadmium (cd), the approach consisted of calculating the hqs and then deriving site-specific assessment criteria (ssac) using the sniffer method. the results suggested that the exposure pathways considered should not engender any form of deleterious health effects for adults. for children, pb was the main concern and induced a relatively high health risk through soil particle ingestion, and most total soil cd concentrations exceeded the derived ssac, in particular, through consumption of vegetables. the metal bioaccessibility in soils was incorporated into the methods to establish more realistic risk assessment measures. this study proposes an approach to integrate different human health risk assessment methods. further investigations should complete the assessment to improve risk determination, e.g., the determination of metal bioaccessibility in vegetables.}, note = {ACL}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {article} } Metal contamination of urban soils and homegrown products has caused major concern. in part 1, we investigated the long-term effects of a former smelter on the degree of kitchen garden-soil contamination and the quality of the homegrown vegetables from these gardens. the results showed that the soils retained a high level of contamination and that a large proportion of the vegetables produced did not comply with the legislation on the levels of metals allowed for human consumption. the present study aims to assess the associated potential health risk to local inhabitants through consumption of homegrown vegetables and ingestion of soil particles using a land use-based approach. for lead (pb), the standard hazard quotient (hq)-based risk assessment method was used to determine the hq. for cadmium (cd), the approach consisted of calculating the hqs and then deriving site-specific assessment criteria (ssac) using the sniffer method. the results suggested that the exposure pathways considered should not engender any form of deleterious health effects for adults. for children, pb was the main concern and induced a relatively high health risk through soil particle ingestion, and most total soil cd concentrations exceeded the derived ssac, in particular, through consumption of vegetables. the metal bioaccessibility in soils was incorporated into the methods to establish more realistic risk assessment measures. this study proposes an approach to integrate different human health risk assessment methods. further investigations should complete the assessment to improve risk determination, e.g., the determination of metal bioaccessibility in vegetables. |
Auteurs : Pelfrêne Aurélie; Grard Olivier; Douay Francis Réduction de l’exposition des populations aux métaux en lien avec le jardinage dans un contexte fortement contaminé de l’ancien bassin minier Conférence Régionale Santé Environnement, 7 juin 2013, Lille @conference{Pelfrene2013c, title = {Réduction de l’exposition des populations aux métaux en lien avec le jardinage dans un contexte fortement contaminé de l’ancien bassin minier}, author = {Aurélie Pelfrêne and Olivier Grard and Francis Douay}, year = {2013}, date = {2013-01-01}, booktitle = {Conférence Régionale Santé Environnement, 7 juin 2013, Lille}, note = {OV}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } |
Auteurs : Pelfrêne Aurélie; Waterlot Christophe; Douay Francis Influence of land use on human bioaccessibility of metals in smelter-impacted soils Environmental Pollution, 178, pp. 80-881 @article{Pelfrene2013a, title = {Influence of land use on human bioaccessibility of metals in smelter-impacted soils}, author = {Aurélie Pelfrêne and Christophe Waterlot and Francis Douay}, year = {2013}, date = {2013-01-01}, journal = {Environmental Pollution}, volume = {178}, pages = {80-88}, abstract = {An investigation was undertaken to evaluate the empirical model developed by pelfrene et al. (2012), predicting the human bioaccessibility of cd and pb in smelter-contaminated agricultural topsoils, by including other soil uses: 50 urban and 65 woody habitat topsoils collected in the same area. the results showed that land use significantly affected the pseudototal metal concentrations and their oral bioaccessibility. however, whatever the soil's physicochemical parameters and degree of contamination, the 'agricultural' model can be used to simulate metal gastric bioaccessibility in urban and woody habitat soils. to simulate gastrointestinal bioaccessibility, this model can be used directly if the pseudototal metal concentrations are on the same order of magnitude as those usually recorded in the agricultural soils studied or after the use of a correction factor if these concentrations are greater. these results showed that the oral bioaccessibility predictions could be applicable for further environmental risk evaluation. (c) 2013 elsevier ltd. all rights reserved.}, note = {ACL}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {article} } An investigation was undertaken to evaluate the empirical model developed by pelfrene et al. (2012), predicting the human bioaccessibility of cd and pb in smelter-contaminated agricultural topsoils, by including other soil uses: 50 urban and 65 woody habitat topsoils collected in the same area. the results showed that land use significantly affected the pseudototal metal concentrations and their oral bioaccessibility. however, whatever the soil's physicochemical parameters and degree of contamination, the 'agricultural' model can be used to simulate metal gastric bioaccessibility in urban and woody habitat soils. to simulate gastrointestinal bioaccessibility, this model can be used directly if the pseudototal metal concentrations are on the same order of magnitude as those usually recorded in the agricultural soils studied or after the use of a correction factor if these concentrations are greater. these results showed that the oral bioaccessibility predictions could be applicable for further environmental risk evaluation. (c) 2013 elsevier ltd. all rights reserved. |
Auteurs : Pourrut Bertrand; Bidar Géraldine; Douay Francis Les plantes face aux polluants: cas des polluants inorganiques Au Pays des Racines, Journée de conférences et d’échanges Société Nationale d’Horticulture de France, 31 janvier 2013, Lomme @conference{Pourrut2013, title = {Les plantes face aux polluants: cas des polluants inorganiques}, author = {Bertrand Pourrut and Géraldine Bidar and Francis Douay}, year = {2013}, date = {2013-01-01}, booktitle = {Au Pays des Racines, Journée de conférences et d’échanges Société Nationale d’Horticulture de France, 31 janvier 2013, Lomme}, note = {OV}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } |
Auteurs : Pourrut Bertrand; Escorza Gabriel; Douay Francis Development of a high-throughput nucleus extraction method on plant tissues 10th International Comet Assay Workshop, 18-20 September 2013, Porto (Portugal) @conference{Pourrut2013a, title = {Development of a high-throughput nucleus extraction method on plant tissues}, author = {Bertrand Pourrut and Gabriel Escorza and Francis Douay}, year = {2013}, date = {2013-01-01}, booktitle = {10th International Comet Assay Workshop, 18-20 September 2013, Porto (Portugal)}, note = {ACTI}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } |
Auteurs : Pourrut Bertrand; Shahid Muhammad; Douay Francis; Dumat Camille; Pinelli Eric Molecular mechanisms involved in lead uptake, toxicity and detoxification in higher plants Dharmendra K. Gupta Francisco J. Corpas José M. Palma, @book{Pourrut2013b, title = {Molecular mechanisms involved in lead uptake, toxicity and detoxification in higher plants}, author = {Bertrand Pourrut and Muhammad Shahid and Francis Douay and Camille Dumat and Eric Pinelli}, year = {2013}, date = {2013-01-01}, booktitle = {in "Heavy Metal Stress in Plants"}, volume = {pp 121-147, Springer}, edition = {Dharmendra K. Gupta Francisco J. Corpas José M. Palma}, abstract = {Lead (pb) is one of the most useful metals, and has contaminated most of the ecosystems. it has been known to influence various morphological, physiological and biochemical processes in plants. higher plants have evolved sophisticated internal detoxification mechanisms, to deal with metal toxicity, that include selective metal uptake, excretion, chelation by specific ligands and compartmentalization. despite these detoxification systems, pb is able to induce the production of reactive oxygen species (ros) which can cause harmful effects on vital constituents of plant cells: protein oxidation, lipid peroxidation, enzyme inactivation or dna damages. unlike other metals, little is known about the mechanisms involved in pb uptake, toxicity, oxidative stress generation and detoxification in plants. this chapter reviews the recent understanding of physiological and biochemical mechanisms involved in pb uptake and translocation into plants. it also provides a broad overview of the most important mechanisms of pb toxicity and tolerance in plants.}, note = {OS}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {book} } Lead (pb) is one of the most useful metals, and has contaminated most of the ecosystems. it has been known to influence various morphological, physiological and biochemical processes in plants. higher plants have evolved sophisticated internal detoxification mechanisms, to deal with metal toxicity, that include selective metal uptake, excretion, chelation by specific ligands and compartmentalization. despite these detoxification systems, pb is able to induce the production of reactive oxygen species (ros) which can cause harmful effects on vital constituents of plant cells: protein oxidation, lipid peroxidation, enzyme inactivation or dna damages. unlike other metals, little is known about the mechanisms involved in pb uptake, toxicity, oxidative stress generation and detoxification in plants. this chapter reviews the recent understanding of physiological and biochemical mechanisms involved in pb uptake and translocation into plants. it also provides a broad overview of the most important mechanisms of pb toxicity and tolerance in plants. |
Auteurs : Scheifler Renaud; Parmentier A -L; Douay Francis Is Miscanthus efficient to reduce trace elements transfer in small mammals on contaminated areas? AquaConSoil, 16-19 April 2013, Barcelona (Espagne) @conference{Scheifler2013, title = {Is Miscanthus efficient to reduce trace elements transfer in small mammals on contaminated areas?}, author = {Renaud Scheifler and A -L Parmentier and Francis Douay}, year = {2013}, date = {2013-01-01}, booktitle = {AquaConSoil, 16-19 April 2013, Barcelona (Espagne)}, note = {ACTI}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } |
Auteurs : Waterlot Christophe; Bidar Géraldine; Pelfrêne Aurélie; Roussel Hélène; Fourrier Hervé; Douay Francis Contamination, fractionation and availability of metals in urban soils in the vicinity of former lead and zinc smelters, France Pedosphere, 23(2), pp. 143-1591 @article{Waterlot2013, title = {Contamination, fractionation and availability of metals in urban soils in the vicinity of former lead and zinc smelters, France}, author = {Christophe Waterlot and Géraldine Bidar and Aurélie Pelfrêne and Hélène Roussel and Hervé Fourrier and Francis Douay}, year = {2013}, date = {2013-01-01}, journal = {Pedosphere}, volume = {23}, number = {2}, pages = {143-159}, abstract = {Soil contamination by metals from anthropogenic activities (e.g., mining and smelting) is a major concern for the environment and human health. environmental availability of cadmium (cd), lead (pb), zinc (zn), copper (cu), and indium (in) in 27 urban soils located around two former pb and zn smelters in northern france were studied by analysing the chemical forms of these metals and evaluating their phytoavailability. these metals were determined using flame or electrothermal absorption atomic spectrometry (faas or etaas), depending on their concentration levels. after optimisation of the etaas method, characteristic mass of in in water and aqua regia were 9.9 and 18 pg, respectively, showing the high sensitivity of the analytical procedure. metal partitioning was conducted using a four-step sequential extraction procedure. the results showed that cd and zn were mainly in the acid-extractable and reducible forms in the urban soils studied. in contrast, pb and in were largely in the reducible fraction. however, in some samples, the amount of in extracted in the residual or exchangeable fraction was higher than that in the reducible fraction. copper was mainly found in the reducible and residual fractions. a pot experiment was conducted in a glasshouse with seven soils (six contaminated and one uncontaminated) and two plant species, ryegrass and lettuce. the results showed transfer of metals from the contaminated soils to the shoots of ryegrass and the edible part of lettuce. the metal bioconcentration factor was in the order of cd >> cu > in > zn >> pb for lettuce leaves, whereas for ryegrass shoots, three orders were found, cd > zn > cu >> in > pb, cd >= in > zn > cu >> pb, and zn > cd > cu > in > pb, depending on the physico-chemical properties of the soils, such as ph, cation exchange capacity, carbonates, and organic matter. it was established that the metal toxicity was related to the contamination levels and the physico-chemical properties, including ph, organic matter, and in a lesser extent, ca, mg, and phosphorus contents, of the soils. however, it was shown that lettuce could grow on soils having high cd and caco3 contents. cadmium was one of the most available metals while pb was always the least available in the soils studied.}, note = {ACL}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {article} } Soil contamination by metals from anthropogenic activities (e.g., mining and smelting) is a major concern for the environment and human health. environmental availability of cadmium (cd), lead (pb), zinc (zn), copper (cu), and indium (in) in 27 urban soils located around two former pb and zn smelters in northern france were studied by analysing the chemical forms of these metals and evaluating their phytoavailability. these metals were determined using flame or electrothermal absorption atomic spectrometry (faas or etaas), depending on their concentration levels. after optimisation of the etaas method, characteristic mass of in in water and aqua regia were 9.9 and 18 pg, respectively, showing the high sensitivity of the analytical procedure. metal partitioning was conducted using a four-step sequential extraction procedure. the results showed that cd and zn were mainly in the acid-extractable and reducible forms in the urban soils studied. in contrast, pb and in were largely in the reducible fraction. however, in some samples, the amount of in extracted in the residual or exchangeable fraction was higher than that in the reducible fraction. copper was mainly found in the reducible and residual fractions. a pot experiment was conducted in a glasshouse with seven soils (six contaminated and one uncontaminated) and two plant species, ryegrass and lettuce. the results showed transfer of metals from the contaminated soils to the shoots of ryegrass and the edible part of lettuce. the metal bioconcentration factor was in the order of cd >> cu > in > zn >> pb for lettuce leaves, whereas for ryegrass shoots, three orders were found, cd > zn > cu >> in > pb, cd >= in > zn > cu >> pb, and zn > cd > cu > in > pb, depending on the physico-chemical properties of the soils, such as ph, cation exchange capacity, carbonates, and organic matter. it was established that the metal toxicity was related to the contamination levels and the physico-chemical properties, including ph, organic matter, and in a lesser extent, ca, mg, and phosphorus contents, of the soils. however, it was shown that lettuce could grow on soils having high cd and caco3 contents. cadmium was one of the most available metals while pb was always the least available in the soils studied. |
Auteurs : Waterlot Christophe; Douay Francis Evaluation des effets d’une lampe à cathode creuse pulsée à courant variable sur les interférences spectrales de l’arsenic dans le dosage du cadmium par spectrophotométrie d’absorption atomique Canadian Journal of Civil Engineering, 8(4), pp. 485-4921 @article{Waterlot2013a, title = {Evaluation des effets d’une lampe à cathode creuse pulsée à courant variable sur les interférences spectrales de l’arsenic dans le dosage du cadmium par spectrophotométrie d’absorption atomique}, author = {Christophe Waterlot and Francis Douay}, year = {2013}, date = {2013-01-01}, journal = {Canadian Journal of Civil Engineering}, volume = {8}, number = {4}, pages = {485-492}, abstract = {Les teneurs en cadmium de solutions aqueuses contaminees a des teneurs croissantes en arsenic ont ete mesurees a l’aide d’un spectrophotome`tre d’absorption atomique equipe d’une flamme et d’un four electrothermique. pour chacune des techniques d’atomisation, a ete evaluee une me´thode de correction du bruit de fond base´e sur le renversement de spectre et connue sous le nom de « high-speed self-reversal method », au regard d’une autre methode plus couramment utilisee ne ne´cessitant qu’une lampe au deute´rium et une lampe au cadmium. en comparaison avec cette me´thode, les re- sultats ont montre que le mode de correction base´ sur le renversement spectral via l’utilisation d’une lampe au cadmium a` cathode creuse pulse´e a` courant variable etait une methode plus approprie´e pour corriger les phe´nome`nes d’interfe´rences causes par l’arsenic (as) lors du dosage du cadmium (cd). il a ete etabli que cette methode permettait de s’affranchir des interferences de l’arsenic pour des ratios [as]/[cd] infe´rieurs a` 50 000.}, note = {ACLN}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {article} } Les teneurs en cadmium de solutions aqueuses contaminees a des teneurs croissantes en arsenic ont ete mesurees a l’aide d’un spectrophotome`tre d’absorption atomique equipe d’une flamme et d’un four electrothermique. pour chacune des techniques d’atomisation, a ete evaluee une me´thode de correction du bruit de fond base´e sur le renversement de spectre et connue sous le nom de « high-speed self-reversal method », au regard d’une autre methode plus couramment utilisee ne ne´cessitant qu’une lampe au deute´rium et une lampe au cadmium. en comparaison avec cette me´thode, les re- sultats ont montre que le mode de correction base´ sur le renversement spectral via l’utilisation d’une lampe au cadmium a` cathode creuse pulse´e a` courant variable etait une methode plus approprie´e pour corriger les phe´nome`nes d’interfe´rences causes par l’arsenic (as) lors du dosage du cadmium (cd). il a ete etabli que cette methode permettait de s’affranchir des interferences de l’arsenic pour des ratios [as]/[cd] infe´rieurs a` 50 000. |
Auteurs : Waterlot Christophe; Douay Francis Minimizing matrix effects and spectral interferences produced by Fe absorption lines in the determination of cadmium by electrothermal atomic absorption spectrometry: Application to the fractionation of cadmium in moderated contaminated soils Measurement, 46, pp. 2348-23581 @article{Waterlot2013b, title = {Minimizing matrix effects and spectral interferences produced by Fe absorption lines in the determination of cadmium by electrothermal atomic absorption spectrometry: Application to the fractionation of cadmium in moderated contaminated soils}, author = {Christophe Waterlot and Francis Douay}, year = {2013}, date = {2013-01-01}, journal = {Measurement}, volume = {46}, pages = {2348-2358}, abstract = {Solutions, including the extracting solutions used in the sequential procedure recommended by the standard, measurements and testing program, with different cadmium and iron concentrations were prepared and analyzed using combined electrothermal atomic absorption spectrometry (etaas) and two background compensation techniques. the first was based on the deuterium background correction method in which a deuterium lamp was used in combination with a conventional cd hollow-cathode lamp. for the second, a high-intensity boosted discharge hollow-cathode lamp (bdhcl) was used as in the smith–hieftje (s–h) background correction method, but this lamp was modulated with a low and a high current mode at a modulation frequency of 100 hz. in the present study, the modulation cycle was 10 times higher than in the s–h system. therefore, the background correction method was called high-speed self-reversal method. pseudo-total cd concentrations were determined using etaas in five reference materials and fractionation was made in two reference soils. with regard to the results, the high-speed self-reversal background correction method provided a method of choice to eliminate or decrease the spectral interference due to the close positioning of the analytical lines of cd and fe in solutions containing high iron concentrations during the cd determination by electrothermal atomic absorption spectrometry. it is worth noting that using the hssr-method, the sensitivity losses were from 32% to 39% compared to the continuum source background corrector equipped with a deuterium lamp. the hssr-method was successfully applied to the determination of cd in contaminated samples. the results showed that interferences caused by absorption line overlapping of cd and fe could be compensated accurately for fe/cd = 100,000 in 0.11 m acetic acid and fe/cd < 10,000 in 0.5 m hydroxylamine hydrochloride.}, note = {ACL}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {article} } Solutions, including the extracting solutions used in the sequential procedure recommended by the standard, measurements and testing program, with different cadmium and iron concentrations were prepared and analyzed using combined electrothermal atomic absorption spectrometry (etaas) and two background compensation techniques. the first was based on the deuterium background correction method in which a deuterium lamp was used in combination with a conventional cd hollow-cathode lamp. for the second, a high-intensity boosted discharge hollow-cathode lamp (bdhcl) was used as in the smith–hieftje (s–h) background correction method, but this lamp was modulated with a low and a high current mode at a modulation frequency of 100 hz. in the present study, the modulation cycle was 10 times higher than in the s–h system. therefore, the background correction method was called high-speed self-reversal method. pseudo-total cd concentrations were determined using etaas in five reference materials and fractionation was made in two reference soils. with regard to the results, the high-speed self-reversal background correction method provided a method of choice to eliminate or decrease the spectral interference due to the close positioning of the analytical lines of cd and fe in solutions containing high iron concentrations during the cd determination by electrothermal atomic absorption spectrometry. it is worth noting that using the hssr-method, the sensitivity losses were from 32% to 39% compared to the continuum source background corrector equipped with a deuterium lamp. the hssr-method was successfully applied to the determination of cd in contaminated samples. the results showed that interferences caused by absorption line overlapping of cd and fe could be compensated accurately for fe/cd = 100,000 in 0.11 m acetic acid and fe/cd < 10,000 in 0.5 m hydroxylamine hydrochloride. |
Auteurs : Douay Francis Le phytomanagement peut-il constituer une solution acceptable sur des sols fortement contaminés par des métaux ? Exemple de Metaleurop Nord Bioindicateurs pour la caractérisation des sols - journée technique nationale. ParisADEME, @conference{Douay2012, title = {Le phytomanagement peut-il constituer une solution acceptable sur des sols fortement contaminés par des métaux ? Exemple de Metaleurop Nord}, author = {Francis Douay}, year = {2012}, date = {2012-01-01}, booktitle = {Bioindicateurs pour la caractérisation des sols - journée technique nationale. Paris}, pages = {10pp}, publisher = {ADEME}, abstract = {1. contexte 2. le site atelier « metaleurop » 3. la gestion des sols agricoles fortement contaminés 4. retour d’expérience sur les phytotechnologies 5. bilan et retombées opérationnelles}, note = {ACTN}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } 1. contexte 2. le site atelier « metaleurop » 3. la gestion des sols agricoles fortement contaminés 4. retour d’expérience sur les phytotechnologies 5. bilan et retombées opérationnelles |
Auteurs : Faure Olivier; Doumas Marc; Conord Cyrille; Bouchardon Jean-Luc; Douay Francis; Leprêtre Alain; Scheifler Renaud Efficience de la phytostabilisation sur le comportement et les transferts des éléments traces métalliques dans les écosystèmes terrestres Bioindicateurs pour la caractérisation des sols - journée technique nationale. ParisADEME, @conference{Faure2012, title = {Efficience de la phytostabilisation sur le comportement et les transferts des éléments traces métalliques dans les écosystèmes terrestres}, author = {Olivier Faure and Marc Doumas and Cyrille Conord and Jean-Luc Bouchardon and Francis Douay and Alain Leprêtre and Renaud Scheifler}, year = {2012}, date = {2012-01-01}, booktitle = {Bioindicateurs pour la caractérisation des sols - journée technique nationale. Paris}, pages = {9pp}, publisher = {ADEME}, abstract = {1. introduction 2. les transferts « vers le bas » : influence de la phytostabilisation sur la quantité et la qualité des eaux d’infiltration 3. les transferts « latéraux » : influence de la phytostabilisation sur la quantité et la qualité des eaux de ruissellement et sur l’envolée de poussières 4. les transferts « vers le haut » : influence de la phytostabilisation sur les transferts vers les réseaux trophiques 5. conclusion}, note = {ACTN}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } 1. introduction 2. les transferts « vers le bas » : influence de la phytostabilisation sur la quantité et la qualité des eaux d’infiltration 3. les transferts « latéraux » : influence de la phytostabilisation sur la quantité et la qualité des eaux de ruissellement et sur l’envolée de poussières 4. les transferts « vers le haut » : influence de la phytostabilisation sur les transferts vers les réseaux trophiques 5. conclusion |
Auteurs : Firmin Stéphane; Verdin Anthony; Philibert E; Pourrut Bertrand; Billet S; Douay Francis; Shirali Pirouz Metal-induced oxidative stress in Miscanthus giganteus grown on a higly contaminated site 9th International Phytotechnologies Society Conference, 12-15 September 2012, Hasselt (Belgique) @conference{Firmin2012, title = {Metal-induced oxidative stress in Miscanthus giganteus grown on a higly contaminated site}, author = {Stéphane Firmin and Anthony Verdin and E Philibert and Bertrand Pourrut and S Billet and Francis Douay and Pirouz Shirali}, year = {2012}, date = {2012-01-01}, booktitle = {9th International Phytotechnologies Society Conference, 12-15 September 2012, Hasselt (Belgique)}, note = {ACTI}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } |
Auteurs : Godet Jean-Philippe; Demuynck Sylvain; Waterlot Christophe; Lemière Sébastien; Souty-Grosset Catherine; Douay Francis; Leprêtre Alain; Pruvot Christelle Fluctuating asymmetry analysis on Porcellio scaber (Crustacea, Isopoda) populations living under metals-contaminated woody habitats Ecological Indicators, 23, pp. 130-1391 @article{Godet2012, title = {Fluctuating asymmetry analysis on Porcellio scaber (Crustacea, Isopoda) populations living under metals-contaminated woody habitats}, author = {Jean-Philippe Godet and Sylvain Demuynck and Christophe Waterlot and Sébastien Lemière and Catherine Souty-Grosset and Francis Douay and Alain Leprêtre and Christelle Pruvot}, year = {2012}, date = {2012-01-01}, journal = {Ecological Indicators}, volume = {23}, pages = {130-139}, abstract = {This field study aimed at determining the effect of metal-polluted environments on fluctuating asymmetry (fa) level of porcellio scaber as a measure of developmental stability (ds). for this, woodlice and litter were collected on 8 sites of northern france located either away from metal pollution source or near metallurgical smelters. physico-chemical parameters such as ph, c/n ratio and caco(3) were measured in litter since they could have potential effects on woodlouse population. moreover, cd, pb, and zn concentrations in litter were determined to evaluate the contamination degree of each site. the metal body burdens were determined in woodlice to evaluate their impregnation degree. fluctuating asymmetry levels on males and gravid females were performed on 9 traits (the 2nd and the 3rd articles of antennae, the 1st flagellum segment of the antennae, and the merus, carpus and propodus of the 6th and the 7th pereiopods) according (1) to each site and (2) to two habitat types (cf. metal-contaminated and uncontaminated habitats). results showed close correlations between litter metal concentrations and metal body burdens in woodlice. however, contrary to the initial hypothesis that metal pollution would induce an increase of the fa level, this seemed to be lower for individuals from metal-contaminated habitats than for those from uncontaminated habitats, and particularly for males. the role of antennae and pereiopods on the foraging behavior and reproduction processes on woodlice were discussed and could explain these observations. (c) 2012 elsevier ltd. all rights reserved.}, note = {ACL}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {article} } This field study aimed at determining the effect of metal-polluted environments on fluctuating asymmetry (fa) level of porcellio scaber as a measure of developmental stability (ds). for this, woodlice and litter were collected on 8 sites of northern france located either away from metal pollution source or near metallurgical smelters. physico-chemical parameters such as ph, c/n ratio and caco(3) were measured in litter since they could have potential effects on woodlouse population. moreover, cd, pb, and zn concentrations in litter were determined to evaluate the contamination degree of each site. the metal body burdens were determined in woodlice to evaluate their impregnation degree. fluctuating asymmetry levels on males and gravid females were performed on 9 traits (the 2nd and the 3rd articles of antennae, the 1st flagellum segment of the antennae, and the merus, carpus and propodus of the 6th and the 7th pereiopods) according (1) to each site and (2) to two habitat types (cf. metal-contaminated and uncontaminated habitats). results showed close correlations between litter metal concentrations and metal body burdens in woodlice. however, contrary to the initial hypothesis that metal pollution would induce an increase of the fa level, this seemed to be lower for individuals from metal-contaminated habitats than for those from uncontaminated habitats, and particularly for males. the role of antennae and pereiopods on the foraging behavior and reproduction processes on woodlice were discussed and could explain these observations. (c) 2012 elsevier ltd. all rights reserved. |
Auteurs : Hayet A; de-Foucault Bruno; Douay Francis; Deram Annabelle Impact sur la biodiversité végétale du Miscanthus cultivé sur des sols contaminés par des éléments traces métalliques Journées d'Etude des Sols. 19-23 mars 2012, Versailles @conference{Hayet2012, title = {Impact sur la biodiversité végétale du Miscanthus cultivé sur des sols contaminés par des éléments traces métalliques}, author = {A Hayet and Bruno de-Foucault and Francis Douay and Annabelle Deram}, year = {2012}, date = {2012-01-01}, booktitle = {Journées d'Etude des Sols. 19-23 mars 2012, Versailles}, note = {ACTN}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } |
Auteurs : Hedde Mickaël; Bispo Antonio; Cluzeau Daniel; Coeurdassier Michaël; Cortet Jérôme; De-Vaufleury Annette; Guernion Muriel; Pauget Benjamin; Ruiz Nuria; Vandenbulcke Franck; Villenave Cécile; Alaphilippe A; Béguiristain Thierry; Douay Francis; Faure Olivier; Hitmi H; Houot Sabine; Legras Marc; Vian Jean-François; Conil S; Rougé Laurence; Pérès Guénola Soil fauna used as bioindicators for soil monitoring, risk assessment and soil characterization. Results from the French national “Bioindicators Programme.” XVI ICSZ – International Colloquium on Soil Zoology, 6-10 August 2012, Coimbra (Portugal) @conference{Hedde2012, title = {Soil fauna used as bioindicators for soil monitoring, risk assessment and soil characterization. Results from the French national “Bioindicators Programme.”}, author = {Mickaël Hedde and Antonio Bispo and Daniel Cluzeau and Michaël Coeurdassier and Jérôme Cortet and Annette De-Vaufleury and Muriel Guernion and Benjamin Pauget and Nuria Ruiz and Franck Vandenbulcke and Cécile Villenave and A Alaphilippe and Thierry Béguiristain and Francis Douay and Olivier Faure and H Hitmi and Sabine Houot and Marc Legras and Jean-François Vian and S Conil and Laurence Rougé and Guénola Pérès}, year = {2012}, date = {2012-01-01}, booktitle = {XVI ICSZ – International Colloquium on Soil Zoology, 6-10 August 2012, Coimbra (Portugal)}, note = {ACTI}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } |
Auteurs : Lucas Jean-Paul; Bretin Philippe; Douay Francis; Etchevers Anne; Glorennec Philippe; Kirchner S; Le-Bot Barbara; Lecoffre C; Le-Strat Yann; Le-Tertre Alain; Mandin Corinne; Oulhote Youssef; Poupon Joël Contamination par le plomb des logements français abritant au moins un enfant âgé de 6 mois à 6 ans Université Paris-Est, Centre Scientifique et Technique du Bâtiment, UPRES EA4275 Université de Nantes, Institut de Veille Sanitaire, Institut Supérieur d’Agriculture de Lille, Ecole des Hautes Etudes en Santé Publique, Hôpital Lariboisière, AP-HP @techreport{Lucas2012b, title = {Contamination par le plomb des logements français abritant au moins un enfant âgé de 6 mois à 6 ans}, author = {Jean-Paul Lucas and Philippe Bretin and Francis Douay and Anne Etchevers and Philippe Glorennec and S Kirchner and Barbara Le-Bot and C Lecoffre and Yann Le-Strat and Alain Le-Tertre and Corinne Mandin and Youssef Oulhote and Joël Poupon}, year = {2012}, date = {2012-01-01}, institution = {Université Paris-Est, Centre Scientifique et Technique du Bâtiment, UPRES EA4275 Université de Nantes, Institut de Veille Sanitaire, Institut Supérieur d’Agriculture de Lille, Ecole des Hautes Etudes en Santé Publique, Hôpital Lariboisière, AP-HP}, abstract = {Contexte la loi du 9 août 2004 relative à la politique de santé publique avait fixé dans ses objectifs de « réduire de 50 % la prévalence du saturnisme infantile (plombémie j 100 kg/l), à savoir passer de 2 % en 1996 à 1 % en 2008 » (objectif n°18). l’indicateur retenu était « le nombre d’enfants de 1 à 6 ans ayant une plombémie j 100 kg/l en population générale et dans les groupes à risque ». en 1996, la prévalence du saturnisme infantile était de 84 000 enfants âgés de 1 an à 6 ans. cependant, seuls 500 cas sont déclarés chaque année. parallèlement, les données collectées par les outils réglementaires relatifs au plomb dans les logements ne sont pas centralisées ; l’état de la contamination en plomb ne peut alors être connu. ainsi, afin d’actualiser les données sur l’imprégnation saturnine infantile, l’institut de veille sanitaire (invs) a réalisé en 2007-2009 une enquête de prévalence du saturnisme chez les enfants de 6 mois à 6 ans. cette enquête a offert l’opportunité d’examiner, à travers une étude nationale complémentaire au domicile de près de 500 enfants, le lien entre la plombémie chez un enfant et les facteurs d'exposition de son environnement. l’enquête environnementale, dénommée plomb-habitat, permet d’avoir une meilleure connaissance de ces déterminants environnementaux, des modes d’occupation du logement et des comportements expliquant l’imprégnation. objectif le présent rapport fournit l’état de la contamination au plomb des logements français, construits avant ou après 1949, abritant au moins un enfant âgé de 6 mois à 6 ans, obtenu à partir de l’enquête plomb-habitat. méthode quatre cent quatre vingt quatre logements ont été enquêtés entre octobre 2008 et août 2009. un questionnaire a permis de collecter des informations relatives au ménage, au comportement de l’enfant et au logement. en outre, des prélèvements de plusieurs sources potentielles d’intoxication par le plomb ont été réalisés : eau du robinet, poussières du sol dans cinq pièces au maximum du logement, poussières du sol dans les parties communes le cas échéant, écailles de peinture et sol extérieur sur l’aire de jeu de l’enfant. des mesures à fluorescence x des revêtements de chaque pièce investiguée ont complété les investigations. l’analyse descriptive réalisée a pris en compte les poids de sondage, permettant ainsi des estimations au niveau national, et a tenu compte du plan de sondage permettant de calculer des incertitudes sur ces estimations. la population de logements ciblée est composée de 3 581 991 unités. l’influence de certains facteurs (âge et localisation du logement, caractéristiques constructives, etc.) sur le plomb présent dans le logement a été testée. la concordance mesure xrf versus concentration massique en plomb dans la peinture a été testée. les ratios concentration en plomb acido-soluble sur concentration en plomb total ont été calculés dans les différents médias.}, note = {VAL}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {techreport} } Contexte la loi du 9 août 2004 relative à la politique de santé publique avait fixé dans ses objectifs de « réduire de 50 % la prévalence du saturnisme infantile (plombémie j 100 kg/l), à savoir passer de 2 % en 1996 à 1 % en 2008 » (objectif n°18). l’indicateur retenu était « le nombre d’enfants de 1 à 6 ans ayant une plombémie j 100 kg/l en population générale et dans les groupes à risque ». en 1996, la prévalence du saturnisme infantile était de 84 000 enfants âgés de 1 an à 6 ans. cependant, seuls 500 cas sont déclarés chaque année. parallèlement, les données collectées par les outils réglementaires relatifs au plomb dans les logements ne sont pas centralisées ; l’état de la contamination en plomb ne peut alors être connu. ainsi, afin d’actualiser les données sur l’imprégnation saturnine infantile, l’institut de veille sanitaire (invs) a réalisé en 2007-2009 une enquête de prévalence du saturnisme chez les enfants de 6 mois à 6 ans. cette enquête a offert l’opportunité d’examiner, à travers une étude nationale complémentaire au domicile de près de 500 enfants, le lien entre la plombémie chez un enfant et les facteurs d'exposition de son environnement. l’enquête environnementale, dénommée plomb-habitat, permet d’avoir une meilleure connaissance de ces déterminants environnementaux, des modes d’occupation du logement et des comportements expliquant l’imprégnation. objectif le présent rapport fournit l’état de la contamination au plomb des logements français, construits avant ou après 1949, abritant au moins un enfant âgé de 6 mois à 6 ans, obtenu à partir de l’enquête plomb-habitat. méthode quatre cent quatre vingt quatre logements ont été enquêtés entre octobre 2008 et août 2009. un questionnaire a permis de collecter des informations relatives au ménage, au comportement de l’enfant et au logement. en outre, des prélèvements de plusieurs sources potentielles d’intoxication par le plomb ont été réalisés : eau du robinet, poussières du sol dans cinq pièces au maximum du logement, poussières du sol dans les parties communes le cas échéant, écailles de peinture et sol extérieur sur l’aire de jeu de l’enfant. des mesures à fluorescence x des revêtements de chaque pièce investiguée ont complété les investigations. l’analyse descriptive réalisée a pris en compte les poids de sondage, permettant ainsi des estimations au niveau national, et a tenu compte du plan de sondage permettant de calculer des incertitudes sur ces estimations. la population de logements ciblée est composée de 3 581 991 unités. l’influence de certains facteurs (âge et localisation du logement, caractéristiques constructives, etc.) sur le plomb présent dans le logement a été testée. la concordance mesure xrf versus concentration massique en plomb dans la peinture a été testée. les ratios concentration en plomb acido-soluble sur concentration en plomb total ont été calculés dans les différents médias. |
Auteurs : Lucas Jean-Paul; Douay Francis Etat de la contamination par le plomb des logements français 7ème colloque francophone sur les sondages, 5-7 novembre 2012, Brutz @conference{Lucas2012a, title = {Etat de la contamination par le plomb des logements français}, author = {Jean-Paul Lucas and Francis Douay}, year = {2012}, date = {2012-01-01}, booktitle = {7ème colloque francophone sur les sondages, 5-7 novembre 2012, Brutz}, note = {ACTI}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } |
Auteurs : Lucas Jean-Paul; Le-Bot Barbara; Glorennec Philippe; Etchevers Anne; Bretin Philippe; Douay Francis; Sébille Véronique; Bellanger Lise; Mandin Corinne Lead contamination in French children's homes and environment Environmental Research, 116, pp. 58-651 @article{Lucas2012c, title = {Lead contamination in French children's homes and environment}, author = {Jean-Paul Lucas and Barbara Le-Bot and Philippe Glorennec and Anne Etchevers and Philippe Bretin and Francis Douay and Véronique Sébille and Lise Bellanger and Corinne Mandin}, year = {2012}, date = {2012-01-01}, journal = {Environmental Research}, volume = {116}, pages = {58-65}, abstract = {Lead in homes is a well-known source of childhood lead exposure, which is still of concern due to the health effects of low lead doses. this study aims to describe lead contamination in the homes of children aged 6 months to 6 years in france (without overseas). between october 2008 and august 2009, 484 housing units were investigated. lead in tap water and total and leachable lead levels from floor dust, outdoor soils and paint chips were measured. x-ray fluorescence measurements were carried out on non-metallic and metallic substrates. nationwide results are provided. the indoor floor dust lead (pbd) geometric mean (gm) was 8.8 mu g/m(2) (0.8 mu g/ft(2)) and 6.8 mu g/m(2) (0.6 mu g/ft(2)) for total and leachable lead respectively; 0.21% of homes had an indoor pbd loading above 430.5 mu g/m(2) (40 mu g/ft(2)). the outdoor play area concentration gm was 33.5 mg/kg and 21.7 mg/kg in total and leachable lead respectively; 1.4% of concentrations were higher than or equal to 400 mg/kg. outdoor floor pbd gm was 44.4 mu g/m(2) (4.1 mu g/ft(2)) that was approximately 3.2 times higher than the gm of indoor pbd. lead-based paint (lbp) was present in 25% of dwellings, lbp on only non-metallic substrates was present in 19% of homes and on metallic substrates in 10% of dwellings. the gm of lead concentrations in tap water was below 1 mu g/l; 58% of concentrations were lower than 1 mu g/l and 2.9% were higher than or equal to 10 mu g/l the age cut-off for homes with lead would be 1974 for paint and 1993 for indoor floor dust. this study provides, for the first time, a look at the state of lead contamination to which children are exposed in french housing. moreover, it provides policy makers an estimate of the number of french dwellings sheltering children where abatement should be conducted. (c) 2012 elsevier inc. all rights reserved.}, note = {ACL}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {article} } Lead in homes is a well-known source of childhood lead exposure, which is still of concern due to the health effects of low lead doses. this study aims to describe lead contamination in the homes of children aged 6 months to 6 years in france (without overseas). between october 2008 and august 2009, 484 housing units were investigated. lead in tap water and total and leachable lead levels from floor dust, outdoor soils and paint chips were measured. x-ray fluorescence measurements were carried out on non-metallic and metallic substrates. nationwide results are provided. the indoor floor dust lead (pbd) geometric mean (gm) was 8.8 mu g/m(2) (0.8 mu g/ft(2)) and 6.8 mu g/m(2) (0.6 mu g/ft(2)) for total and leachable lead respectively; 0.21% of homes had an indoor pbd loading above 430.5 mu g/m(2) (40 mu g/ft(2)). the outdoor play area concentration gm was 33.5 mg/kg and 21.7 mg/kg in total and leachable lead respectively; 1.4% of concentrations were higher than or equal to 400 mg/kg. outdoor floor pbd gm was 44.4 mu g/m(2) (4.1 mu g/ft(2)) that was approximately 3.2 times higher than the gm of indoor pbd. lead-based paint (lbp) was present in 25% of dwellings, lbp on only non-metallic substrates was present in 19% of homes and on metallic substrates in 10% of dwellings. the gm of lead concentrations in tap water was below 1 mu g/l; 58% of concentrations were lower than 1 mu g/l and 2.9% were higher than or equal to 10 mu g/l the age cut-off for homes with lead would be 1974 for paint and 1993 for indoor floor dust. this study provides, for the first time, a look at the state of lead contamination to which children are exposed in french housing. moreover, it provides policy makers an estimate of the number of french dwellings sheltering children where abatement should be conducted. (c) 2012 elsevier inc. all rights reserved. |
Auteurs : Pauget Benjamin; Coeurdassier Michaël; Gimbert Frédéric; Pérès Guénola; Alaphilippe A; Béguiristain Thierry; Douay Francis; Faure Olivier; Hitmi Adnane; Houot Sabine; Legras Marc; Guernion Muriel; Vian Jean-François; De-Vaufleury Annette Snail watch: a tool to assess the risk of metal transfer taking into account their bioavailability SETAC. 20-24 May 2012, Berlin (Allemagne) @conference{Pauget2012, title = {Snail watch: a tool to assess the risk of metal transfer taking into account their bioavailability}, author = {Benjamin Pauget and Michaël Coeurdassier and Frédéric Gimbert and Guénola Pérès and A Alaphilippe and Thierry Béguiristain and Francis Douay and Olivier Faure and Adnane Hitmi and Sabine Houot and Marc Legras and Muriel Guernion and Jean-François Vian and Annette De-Vaufleury}, year = {2012}, date = {2012-01-01}, booktitle = {SETAC. 20-24 May 2012, Berlin (Allemagne)}, note = {ACTI}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } |
Auteurs : Pelfrêne Aurélie; Waterlot Christophe; Douay Francis Validation and first deployment of the DGT technique in artificial human gastrointestinal fluids after ingestion of metal-containing soil particles SETAC. 20-24 May 2012, Berlin (Allemagne) @conference{Pelfrene2012b, title = {Validation and first deployment of the DGT technique in artificial human gastrointestinal fluids after ingestion of metal-containing soil particles}, author = {Aurélie Pelfrêne and Christophe Waterlot and Francis Douay}, year = {2012}, date = {2012-01-01}, booktitle = {SETAC. 20-24 May 2012, Berlin (Allemagne)}, note = {ACTI}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } |
Auteurs : Pelfrêne Aurélie; Waterlot Christophe; Mazzuca Muriel; Nisse Catherine; Bidar Géraldine; Douay Francis Modélisation de la bioaccessibilité orale de Cd et Pb au regard des paramètres physico-chimiques de sols agricoles fortement contaminés : Application à un site contaminé du Nord-Pas de Calais Intersol. 27-30 mars 2012, Paris @conference{Pelfrene2012c, title = {Modélisation de la bioaccessibilité orale de Cd et Pb au regard des paramètres physico-chimiques de sols agricoles fortement contaminés : Application à un site contaminé du Nord-Pas de Calais}, author = {Aurélie Pelfrêne and Christophe Waterlot and Muriel Mazzuca and Catherine Nisse and Géraldine Bidar and Francis Douay}, year = {2012}, date = {2012-01-01}, booktitle = {Intersol. 27-30 mars 2012, Paris}, note = {ACTN}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } |
Auteurs : Pelfrêne Aurélie; Waterlot Christophe; Mazzuca Muriel; Nisse Catherine; Cuny Damien; Richard Antoine; Denys Sébastien; Heyman Christophe; Roussel Hélène; Bidar Géraldine; Douay Francis Bioaccessibility of trace elements as affected by soil parameters in smelter-contaminated agricultural soils: A statistical modeling approach Environmental Pollution, 160, pp. 130-1381 @article{Pelfrene2012a, title = {Bioaccessibility of trace elements as affected by soil parameters in smelter-contaminated agricultural soils: A statistical modeling approach}, author = {Aurélie Pelfrêne and Christophe Waterlot and Muriel Mazzuca and Catherine Nisse and Damien Cuny and Antoine Richard and Sébastien Denys and Christophe Heyman and Hélène Roussel and Géraldine Bidar and Francis Douay}, year = {2012}, date = {2012-01-01}, journal = {Environmental Pollution}, volume = {160}, pages = {130-138}, abstract = {An investigation was undertaken to identify the most significant soil parameters that can be used to predict cd, pb, and zn bioaccessibility in smelter-contaminated agricultural soils. a robust model was established from an extended database of soils by using: (i) a training set of 280 samples to select the main soil parameters, to define the best population to be taken into account for the model elaboration, and to construct multivariate regression models, and (ii) a test set of 110 samples to validate the ability of the regression models. total carbonate, organic matter, sand, p2o5, free feemn oxide, and pseudototal al and trace element (te) contents appeared as the main variables governing te bioaccessibility. the statistical modeling approach was reasonably successful, indicating that the main soil factors influencing the bioaccessibility of tes were taken into account and the predictions could be applicable for further risk evaluation in the studied area.}, note = {ACL}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {article} } An investigation was undertaken to identify the most significant soil parameters that can be used to predict cd, pb, and zn bioaccessibility in smelter-contaminated agricultural soils. a robust model was established from an extended database of soils by using: (i) a training set of 280 samples to select the main soil parameters, to define the best population to be taken into account for the model elaboration, and to construct multivariate regression models, and (ii) a test set of 110 samples to validate the ability of the regression models. total carbonate, organic matter, sand, p2o5, free feemn oxide, and pseudototal al and trace element (te) contents appeared as the main variables governing te bioaccessibility. the statistical modeling approach was reasonably successful, indicating that the main soil factors influencing the bioaccessibility of tes were taken into account and the predictions could be applicable for further risk evaluation in the studied area. |
Auteurs : Pérès Guénola; Bispo Antonio; Grand Cécile; Galsomies Laurence; Gattin Isabelle; Harris-Hellal Jennifer; Hedde Mickaël; Le-Guédard Marina; Ruiz Nuria; Alaphilippe A; Béguiristain Thierry; Conil S; Douay Francis; Faure Olivier; Guernion Muriel; Hitmi Adnane; Houot Sabine; Legras Marc; Vian Jean-François; Dur Jeanne-Chantal; Lepelletier Patrice; Rougé Laurence; Taibi Salima; Cluzeau Daniel Utilisation des Biomarqueurs et Bioindicateurs dans la surveillance de la qualité des sols et l’évaluation des risques Journées d'Etude des Sols. 19-23 mars 2012, Versailles @conference{Peres2012a, title = {Utilisation des Biomarqueurs et Bioindicateurs dans la surveillance de la qualité des sols et l’évaluation des risques}, author = {Guénola Pérès and Antonio Bispo and Cécile Grand and Laurence Galsomies and Isabelle Gattin and Jennifer Harris-Hellal and Mickaël Hedde and Marina Le-Guédard and Nuria Ruiz and A Alaphilippe and Thierry Béguiristain and S Conil and Francis Douay and Olivier Faure and Muriel Guernion and Adnane Hitmi and Sabine Houot and Marc Legras and Jean-François Vian and Jeanne-Chantal Dur and Patrice Lepelletier and Laurence Rougé and Salima Taibi and Daniel Cluzeau}, year = {2012}, date = {2012-01-01}, booktitle = {Journées d'Etude des Sols. 19-23 mars 2012, Versailles}, note = {ACTN}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } |
Auteurs : Pérès Guénola; Bispo Antonio; Grand Cécile; Gattin Isabelle; Hedde Mickaël; Harris-Hellal Jennifer; Le-Guédard Marina; Ruiz Nuria; Alaphilippe A; Béguiristain Thierry; Douay Francis; Faure Olivier; Hitmi Adnane; Houot Sabine; Legras Marc; Guernion Muriel; Vian Jean-François; Rougé Laurence; Lepelletier Patrice; Cluzeau Daniel Soil bioindicators for soil monitoring, risk assessment and soil characterization. Results from the French national “Bioindicators Programme” Eurosoil, 2-6 July 2012, Bari (Italy) @conference{Peres2012b, title = {Soil bioindicators for soil monitoring, risk assessment and soil characterization. Results from the French national “Bioindicators Programme”}, author = {Guénola Pérès and Antonio Bispo and Cécile Grand and Isabelle Gattin and Mickaël Hedde and Jennifer Harris-Hellal and Marina Le-Guédard and Nuria Ruiz and A Alaphilippe and Thierry Béguiristain and Francis Douay and Olivier Faure and Adnane Hitmi and Sabine Houot and Marc Legras and Muriel Guernion and Jean-François Vian and Laurence Rougé and Patrice Lepelletier and Daniel Cluzeau}, year = {2012}, date = {2012-01-01}, booktitle = {Eurosoil, 2-6 July 2012, Bari (Italy)}, note = {ACTI}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } |
Auteurs : Pérès Guénola; Vandenbulcke Franck; Guernion Muriel; Hedde Mickaël; Béguiristain Thierry; Douay Francis; Houot Sabine; Piron Denis; Richard Antoine; Bispo Antonio; Grand Cécile; Galsomies Laurence; Cluzeau Daniel Earthworm indicators as tools for soil monitoring, characterization and risk assessment. An example from the national Bioindicator programme (France) Pedobiologia, 54, pp. S77-S871 @article{Peres2012c, title = {Earthworm indicators as tools for soil monitoring, characterization and risk assessment. An example from the national Bioindicator programme (France)}, author = {Guénola Pérès and Franck Vandenbulcke and Muriel Guernion and Mickaël Hedde and Thierry Béguiristain and Francis Douay and Sabine Houot and Denis Piron and Antoine Richard and Antonio Bispo and Cécile Grand and Laurence Galsomies and Daniel Cluzeau}, year = {2012}, date = {2012-01-01}, journal = {Pedobiologia}, volume = {54}, pages = {S77-S87}, abstract = {Considering the limited number of bioindicators available to assess soil quality, a national research programme was set up in france to develop such indicators (2006-2012), the "bioindicator" programme.this programme tested 47 biological parameters (i.e. microorganisms, fauna, flora) including earthworms, in several sites differing in terms of land use, contamination type - pahs or metals - and pollution levels. the present study proposes some study objectives for bioindicator approaches, based on the earthwormn results from the programme. therefore, different earthworm descriptors were tested at the community level (e.g. abundance, biomass, species and functional structures, and ecological traits) as well as the organism level (i.e. measuring the metallothionein coding gene expression level in earthworms). the present results, obtained from the programme's spring 2009 sampling campaign, discriminated among the different descriptors and showed that earthworm and endogeic abundance as well as the individual weight of endogeics seem to be good indicators in non-contaminated (cultivated) sites, while the ecological structure, namely the proportion of anecic vs. endogeic species, and the proportion of non-vulnerable species should be used as indicators of contaminated soils. furthermore, the first results obtained for lumbricus terrestris and l rubellus rubellus are encouraging as they show that metallothionein expression increases in metal-contaminated soils. the relevance of these descriptors, which have to be considered in study objectives, requires the analysis of 2010 results. (c) 2011 elsevier gmbh. all rights reserved.}, note = {ACL}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {article} } Considering the limited number of bioindicators available to assess soil quality, a national research programme was set up in france to develop such indicators (2006-2012), the "bioindicator" programme.this programme tested 47 biological parameters (i.e. microorganisms, fauna, flora) including earthworms, in several sites differing in terms of land use, contamination type - pahs or metals - and pollution levels. the present study proposes some study objectives for bioindicator approaches, based on the earthwormn results from the programme. therefore, different earthworm descriptors were tested at the community level (e.g. abundance, biomass, species and functional structures, and ecological traits) as well as the organism level (i.e. measuring the metallothionein coding gene expression level in earthworms). the present results, obtained from the programme's spring 2009 sampling campaign, discriminated among the different descriptors and showed that earthworm and endogeic abundance as well as the individual weight of endogeics seem to be good indicators in non-contaminated (cultivated) sites, while the ecological structure, namely the proportion of anecic vs. endogeic species, and the proportion of non-vulnerable species should be used as indicators of contaminated soils. furthermore, the first results obtained for lumbricus terrestris and l rubellus rubellus are encouraging as they show that metallothionein expression increases in metal-contaminated soils. the relevance of these descriptors, which have to be considered in study objectives, requires the analysis of 2010 results. (c) 2011 elsevier gmbh. all rights reserved. |
Auteurs : Pernin Céline; Demuynck Sylvain; Douay Francis; Grumiaux Fabien; Guillemand David; Leprêtre Alain; Pohu Alena; Pourrut Bertrand; Pruvot Christelle Une restauration de la biodiversité aux alentours de l’ancienne fonderie Metaleurop Nord est-elle envisageable? Quelques pistes... Premières journées techniques du réseau SAFIR. 11-12 mai 2012, Roubaix, @article{Pernin2012, title = {Une restauration de la biodiversité aux alentours de l’ancienne fonderie Metaleurop Nord est-elle envisageable? Quelques pistes...}, author = {Céline Pernin and Sylvain Demuynck and Francis Douay and Fabien Grumiaux and David Guillemand and Alain Leprêtre and Alena Pohu and Bertrand Pourrut and Christelle Pruvot}, year = {2012}, date = {2012-01-01}, journal = {Premières journées techniques du réseau SAFIR. 11-12 mai 2012, Roubaix}, note = {COM}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {article} } |
Auteurs : Schwartz Christophe; Chenot Elodie-Denise; Cortet Jérôme; Douay Francis; Dumat Camille; Pernin Céline; Pourrut Bertrand A strategy for the survey of urban garden soils European Geosciences Union. 22–27 April 2012, Vienna (Autriche) @conference{Schwartz2012, title = {A strategy for the survey of urban garden soils}, author = {Christophe Schwartz and Elodie-Denise Chenot and Jérôme Cortet and Francis Douay and Camille Dumat and Céline Pernin and Bertrand Pourrut}, year = {2012}, date = {2012-01-01}, booktitle = {European Geosciences Union. 22–27 April 2012, Vienna (Autriche)}, note = {ACTI}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } |
Auteurs : Sterckeman Thibault; Villanneau Estelle; Bourennane Hocine; Douay Francis; Ciesielski Henri; King Dominique; Baize Denis Les éléments en traces dans les sols agricoles du Nord-Pas de Calais. II. Cartographie des enrichissements dans les horizons de surface Etude et Gestion des Sols, 19(3-4), pp. 163-1781 @article{Sterckeman2012, title = {Les éléments en traces dans les sols agricoles du Nord-Pas de Calais. II. Cartographie des enrichissements dans les horizons de surface}, author = {Thibault Sterckeman and Estelle Villanneau and Hocine Bourennane and Francis Douay and Henri Ciesielski and Dominique King and Denis Baize}, year = {2012}, date = {2012-01-01}, journal = {Etude et Gestion des Sols}, volume = {19}, number = {3-4}, pages = {163-178}, abstract = {La région nord - pas de calais est une région très densément peuplée qui a été profondément affectée par des industries lourdes, l’exploitation minière du charbon dans le passé et une agriculture intensive, toujours active. un jeu de données a été constitué en rassemblant des teneurs en éléments en traces mesurées dans le cadre de différentes études menées sur les sols agricoles de la région. des facteurs d’enrichissements (fe) ont été calculés en chaque site pour 18 éléments en traces et 250 solums en utilisant l’aluminium comme élément géochimique de référence et un horizon profond ou le matériau parental supposé non contaminé par les activités humaines. l’analyse variographique a révélé que seulement huit métaux traces (bi, cd, cu, in, pb, sn, tl, zn) sont spatialement corrélés, ce qui a permis d’utiliser la simulation séquentielle gaussienne pour la spatialisation de leurs fe. le cd montre un net enrichissement (fe le plus souvent compris entre 3 et 10, parfois plus) sur la quasi-totalité du territoire régional tandis que le zn et le pb montrent de forts enrichissements localement (fe de 3 à 30) à proximité immédiate d’usines de production de ces métaux (auby, noyelles-godault, mortagne-du-nord) ou de grandes agglomérations (dunkerque, lille, valenciennes, est du bassin minier). le bi, le cu et le sn montrent des enrichissements modérés (de 1,5 à 3) sur la moitié du territoire régional, principalement autour des plus grandes villes et des usines de production de pb et zn. l’indium et le tl présentent des fe de 1 à 1,5 sur l’ensemble du territoire avec des valeurs plus élevées en certaines zones urbanisées ou industrialisées. les cartes de spatialisation des fe, rapprochées de celle de l’occupation du territoire par les activités humaines, montrent les origines principalement industrielle, urbaine et routière des enrichissements en pb et zn, de même que ceux, nettement plus faibles en bi, in et tl. les enrichissements en cu, sn et cd comporteraient quant à eux une origine agricole plus importante que ceux des précédents éléments. certains éléments non spatialement corrélés présentent des fe nettement supérieurs à 1 en moyenne. c’est le cas de hg, mn, mo, sb et se. ces enrichissements ont probablement en partie une origine humaine mais l’impossibilité de modéliser leur distribution spatiale n’a pas permis de les relier aux activités anthropiques. enfin, as, co, cr, ni et v ne semblent pas avoir été apportés dans les sols par les activités humaines, puisqu’ils présentent des fe proches de 1 et, de plus, non reliés à l’occupation du territoire. pour certains éléments, les quantités apportées par les activités humaines et stockées dans l’horizon de surface ont été estimées. les plus importantes sont celles du pb et du zn, de l’ordre de 60 000 t. viennent ensuite le cu, avec environ 15 000 t, le sn (environ 2 000 t) et le cd (environ 1 100 t).}, note = {ACLN}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {article} } La région nord - pas de calais est une région très densément peuplée qui a été profondément affectée par des industries lourdes, l’exploitation minière du charbon dans le passé et une agriculture intensive, toujours active. un jeu de données a été constitué en rassemblant des teneurs en éléments en traces mesurées dans le cadre de différentes études menées sur les sols agricoles de la région. des facteurs d’enrichissements (fe) ont été calculés en chaque site pour 18 éléments en traces et 250 solums en utilisant l’aluminium comme élément géochimique de référence et un horizon profond ou le matériau parental supposé non contaminé par les activités humaines. l’analyse variographique a révélé que seulement huit métaux traces (bi, cd, cu, in, pb, sn, tl, zn) sont spatialement corrélés, ce qui a permis d’utiliser la simulation séquentielle gaussienne pour la spatialisation de leurs fe. le cd montre un net enrichissement (fe le plus souvent compris entre 3 et 10, parfois plus) sur la quasi-totalité du territoire régional tandis que le zn et le pb montrent de forts enrichissements localement (fe de 3 à 30) à proximité immédiate d’usines de production de ces métaux (auby, noyelles-godault, mortagne-du-nord) ou de grandes agglomérations (dunkerque, lille, valenciennes, est du bassin minier). le bi, le cu et le sn montrent des enrichissements modérés (de 1,5 à 3) sur la moitié du territoire régional, principalement autour des plus grandes villes et des usines de production de pb et zn. l’indium et le tl présentent des fe de 1 à 1,5 sur l’ensemble du territoire avec des valeurs plus élevées en certaines zones urbanisées ou industrialisées. les cartes de spatialisation des fe, rapprochées de celle de l’occupation du territoire par les activités humaines, montrent les origines principalement industrielle, urbaine et routière des enrichissements en pb et zn, de même que ceux, nettement plus faibles en bi, in et tl. les enrichissements en cu, sn et cd comporteraient quant à eux une origine agricole plus importante que ceux des précédents éléments. certains éléments non spatialement corrélés présentent des fe nettement supérieurs à 1 en moyenne. c’est le cas de hg, mn, mo, sb et se. ces enrichissements ont probablement en partie une origine humaine mais l’impossibilité de modéliser leur distribution spatiale n’a pas permis de les relier aux activités anthropiques. enfin, as, co, cr, ni et v ne semblent pas avoir été apportés dans les sols par les activités humaines, puisqu’ils présentent des fe proches de 1 et, de plus, non reliés à l’occupation du territoire. pour certains éléments, les quantités apportées par les activités humaines et stockées dans l’horizon de surface ont été estimées. les plus importantes sont celles du pb et du zn, de l’ordre de 60 000 t. viennent ensuite le cu, avec environ 15 000 t, le sn (environ 2 000 t) et le cd (environ 1 100 t). |
Auteurs : Villenave Cécile; Pérès Guénola; Alaphilippe A; Béguiristain Thierry; Bispo Bruno; Cortet Jérôme; Douay Francis; Faure Olivier; Galsomies Laurence; Guernion Muriel; Grand Cécile; Hedde H; Hitmi H; Houot Sabine; Legras Marc; Ruiz Nuria; Vian Jean-François Nematofauna study for soil monitoring: results from the French national “Bioindicators Programme.” Second International Symposium on nematodes as environmental bio-indicators. 5-6 July 2012, Gent (Belgique) @conference{Villenave2012, title = {Nematofauna study for soil monitoring: results from the French national “Bioindicators Programme.”}, author = {Cécile Villenave and Guénola Pérès and A Alaphilippe and Thierry Béguiristain and Bruno Bispo and Jérôme Cortet and Francis Douay and Olivier Faure and Laurence Galsomies and Muriel Guernion and Cécile Grand and H Hedde and H Hitmi and Sabine Houot and Marc Legras and Nuria Ruiz and Jean-François Vian}, year = {2012}, date = {2012-01-01}, booktitle = {Second International Symposium on nematodes as environmental bio-indicators. 5-6 July 2012, Gent (Belgique)}, note = {ACTI}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } |
Auteurs : Villenave Cécile; Pérès Guénola; Alaphilippe A; Béguiristain Thierry; Bispo Bruno; Douay Francis; Faure Olivier; Guernion Muriel; Grand Cécile; Hedde H; Hitmi H; Houot Sabine; Legras Marc; Ruiz Nuria; Vian Jean-François Nematofauna study for soil monitoring, risk assessment and soil characterization. Results from the French national “Bioindicators Programme"; Eurosoil, 2-6 July 2012, Bari (Italy) @conference{Villenave2012a, title = {Nematofauna study for soil monitoring, risk assessment and soil characterization. Results from the French national “Bioindicators Programme"; Eurosoil, 2-6 July 2012, Bari (Italy)}, author = {Cécile Villenave and Guénola Pérès and A Alaphilippe and Thierry Béguiristain and Bruno Bispo and Francis Douay and Olivier Faure and Muriel Guernion and Cécile Grand and H Hedde and H Hitmi and Sabine Houot and Marc Legras and Nuria Ruiz and Jean-François Vian}, year = {2012}, date = {2012-01-01}, note = {ACTI}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } |
Auteurs : Waterlot Christophe; Bidar Géraldine; Pruvot Christelle; Douay Francis Effects of grinding and shaking on Cd, Pb and Zn distribution in anthropogenically impacted soils Talanta, 98, pp. 185-1961 @article{Waterlot2012, title = {Effects of grinding and shaking on Cd, Pb and Zn distribution in anthropogenically impacted soils}, author = {Christophe Waterlot and Géraldine Bidar and Christelle Pruvot and Francis Douay}, year = {2012}, date = {2012-01-01}, journal = {Talanta}, volume = {98}, pages = {185-196}, abstract = {The effectsofgrinding size andshaking process on the results ofcd (cadmium), pb (lead) and zn (zinc) distribution measurements three agricultural and three kitchen garden soils highly contaminated by past atmospheric fallout of two lead and zinc smelters in northern france were studied. the physico-chemical parameters and pseudo-total concentration of metals within these soils were determined. the fractionation of metals was performed in triplicate, using the procedure recommended by the standards, measurements and testing program (sm&t), on each air-dried soil sample, ground to pass through 2-mm, 0.315-mm and 0.250-mm sieves and using a reciprocating or rotary shaker. the samples were analysed by flame or electrothermal absorption atomic spectrometry using a self-reversal background system. for both shaking processes, the grinding size had no effecton the fractionation of metals in contaminated agricultural soils. in contrast, using a reciprocating shaker, the fractionation of metals in the kitchen garden samples sieved at <2 mm was so different that in the samples prepared to pass through the 0.315-mm and 0.250-mm sieves. therefore changes (use of a 50 ml graduated polypropylene centrifuge tube, evaporation of the solution to a fixed volume in step 3 and the use of an automatic shaking heating bath) were made to the initial procedure and a rotary shaker was used to improve the suspension of the soil samples during extraction. for all grinding sizes, the fractionation of the three metals contained in the contaminated kitchen garden soil samples was successfully achieved. nevertheless, some discrepancies from samples sieved at <2 mm were obtained. on the other hand, it is worth noting that the effectof the type of shaker on the distribution of metal depended on the soil and the grinding size. from an analytical point of view, precision and trueness were improved after optimisation of the procedure for all sequential extraction procedure steps. the best results were obtained for samples sieved at <0.250 mm. similar trends were obtained using the crm bcr®-701 certified material. all the results showed that optimisation of the sequential extraction procedure could be adopted for the cd-, pb- and zn-partitioning in contaminated kitchen garden soils with high-level anthropogenic sources.}, note = {ACL}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {article} } The effectsofgrinding size andshaking process on the results ofcd (cadmium), pb (lead) and zn (zinc) distribution measurements three agricultural and three kitchen garden soils highly contaminated by past atmospheric fallout of two lead and zinc smelters in northern france were studied. the physico-chemical parameters and pseudo-total concentration of metals within these soils were determined. the fractionation of metals was performed in triplicate, using the procedure recommended by the standards, measurements and testing program (sm&t), on each air-dried soil sample, ground to pass through 2-mm, 0.315-mm and 0.250-mm sieves and using a reciprocating or rotary shaker. the samples were analysed by flame or electrothermal absorption atomic spectrometry using a self-reversal background system. for both shaking processes, the grinding size had no effecton the fractionation of metals in contaminated agricultural soils. in contrast, using a reciprocating shaker, the fractionation of metals in the kitchen garden samples sieved at <2 mm was so different that in the samples prepared to pass through the 0.315-mm and 0.250-mm sieves. therefore changes (use of a 50 ml graduated polypropylene centrifuge tube, evaporation of the solution to a fixed volume in step 3 and the use of an automatic shaking heating bath) were made to the initial procedure and a rotary shaker was used to improve the suspension of the soil samples during extraction. for all grinding sizes, the fractionation of the three metals contained in the contaminated kitchen garden soil samples was successfully achieved. nevertheless, some discrepancies from samples sieved at <2 mm were obtained. on the other hand, it is worth noting that the effectof the type of shaker on the distribution of metal depended on the soil and the grinding size. from an analytical point of view, precision and trueness were improved after optimisation of the procedure for all sequential extraction procedure steps. the best results were obtained for samples sieved at <0.250 mm. similar trends were obtained using the crm bcr®-701 certified material. all the results showed that optimisation of the sequential extraction procedure could be adopted for the cd-, pb- and zn-partitioning in contaminated kitchen garden soils with high-level anthropogenic sources. |
Auteurs : Waterlot Christophe; Douay Francis Minimizing Chloride Interferences Produced by Calcium Chloride in the Determination of Cd by Graphite Furnace Atomic Absorption Spectrometry ISRN Spectroscopy, pp. 1-101 @article{Waterlot2012a, title = {Minimizing Chloride Interferences Produced by Calcium Chloride in the Determination of Cd by Graphite Furnace Atomic Absorption Spectrometry}, author = {Christophe Waterlot and Francis Douay}, year = {2012}, date = {2012-01-01}, journal = {ISRN Spectroscopy}, pages = {1-10}, abstract = {Cadmium concentrations in cacl2 extracting solutions at various concentrations were determined by graphite furnace atomic absorption using two background correctors: the deuterium and the high-speed self-reversal background correction systems. under- and overestimation of the cd absorbance signals in cacl2 solutions were observed for concentrations greater than 0.005m using the deuterium lamp while no important effect was observed using the other background correction system. the analytical performance of the spectrometer for the determination of cd was studied in 0.01m cacl2 solution and single extractions were performed using reference materials and contaminated soil samples. cadmium was determined using the two background correction systems and a third method, which consists of the use of the deuterium lamp without any chemical modifier, was added to the study. the results showed that the thirdmethod was unable to determine cd concentrations in the cacl2 solution due to the presence of extractable arsenic and iron. for solutions without any dilution or diluted with a very low dilution factor, the cacl2- extractable cd concentrations measured using the deuterium lamp were systematically below those found using the high-speed self-reversal method. these differences were explained by the presence of chloride ions in the atomization step.}, note = {ACLO}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {article} } Cadmium concentrations in cacl2 extracting solutions at various concentrations were determined by graphite furnace atomic absorption using two background correctors: the deuterium and the high-speed self-reversal background correction systems. under- and overestimation of the cd absorbance signals in cacl2 solutions were observed for concentrations greater than 0.005m using the deuterium lamp while no important effect was observed using the other background correction system. the analytical performance of the spectrometer for the determination of cd was studied in 0.01m cacl2 solution and single extractions were performed using reference materials and contaminated soil samples. cadmium was determined using the two background correction systems and a third method, which consists of the use of the deuterium lamp without any chemical modifier, was added to the study. the results showed that the thirdmethod was unable to determine cd concentrations in the cacl2 solution due to the presence of extractable arsenic and iron. for solutions without any dilution or diluted with a very low dilution factor, the cacl2- extractable cd concentrations measured using the deuterium lamp were systematically below those found using the high-speed self-reversal method. these differences were explained by the presence of chloride ions in the atomization step. |
Auteurs : Waterlot Christophe; Douay Francis; Daurangeon Fabien La spectrométrie d’absorption atomique par électrothermie: une technique d’analyse simple pour la détermination de l’indium dans les sols contaminés Spectra Analyse, 289, pp. 24-281 @article{Waterlot2012b, title = {La spectrométrie d’absorption atomique par électrothermie: une technique d’analyse simple pour la détermination de l’indium dans les sols contaminés}, author = {Christophe Waterlot and Francis Douay and Fabien Daurangeon}, year = {2012}, date = {2012-01-01}, journal = {Spectra Analyse}, volume = {289}, pages = {24-28}, note = {ACLN}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {article} } |
Auteurs : Waterlot Christophe; Pelfrêne Aurélie; Douay Francis Application of the high-speed self-reversal background corrector to the determination of cadmium by chemical vapor generation atomic absorption spectrometry Canadian Journal of Chemistry, 90, pp. 1-61 @article{Waterlot2012c, title = {Application of the high-speed self-reversal background corrector to the determination of cadmium by chemical vapor generation atomic absorption spectrometry}, author = {Christophe Waterlot and Aurélie Pelfrêne and Francis Douay}, year = {2012}, date = {2012-01-01}, journal = {Canadian Journal of Chemistry}, volume = {90}, pages = {1-6}, abstract = {Les concentrations en cadmium (cd) dans les solutions d’extraction (sels neutres) de sols contaminés sont souvent trop faibles pour pouvoir être déterminées par spectrométrie d’absorption atomique en utilisant une simple flamme pour atomiser les éléments. la quantification de cd a` l’état de traces requière donc des méthodes d’analyses sensibles mais aussi exemptes d’interférences. dans ce contexte, le dosage de cd a été réalisé au moyen d’un spectromètre d’absorption atomique couplé a` un générateur d’hydrure en mode continu et combiné a` une méthode de correction du bruit de fond par renversement de spectre. les conditions optimales d’utilisation de la technique ont été déterminées de manière a` obtenir une sensibilité maximale. pour des concentrations de 2,5 et 5 ng ml–1, les absorbances maximales de cd ont été obtenues en préparant une solution de nabh4 a` 3 % dans une solution de soude a` 1,5 % et en analysant des solutions dont la concentration en hno3 a été de 0,3 mol/l. dans ces conditions, la limite de détection a été de 1 ng ml–1 et un coefficient de variation de 5 % a été trouvé en analysant a` 10 reprises des solutions de cd a` 0,2 et 1 ng ml–1, montrant ainsi une bonne sensibilité et reproductibilité de la méthode. de plus, la méthode analytique proposée s’est avérée être une méthode efficace permettant de minimiser les interférences causées par les cations as, al, ca, fe, mn, ni, pb, se et zn jusqu’a` 10 g ml–1. la méthode a été validée par la détermination des concentrations en cd dans les solutions d’extraction au cacl2 0,01 mol/l de sols certifiés et de sols contaminés. les données acquises avec la méthode proposées ont été comparées a` celles obtenues en utilisant des méthodes d’analyses plus conventionnelles, spectrométrie absorption atomique avec atomisation électrothermique et flamme. l’ensemble des résultats obtenus a montré que la méthode proposée était sensible, reproductible et permettait de s’affranchir de certaines interférences.}, note = {ACL}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {article} } Les concentrations en cadmium (cd) dans les solutions d’extraction (sels neutres) de sols contaminés sont souvent trop faibles pour pouvoir être déterminées par spectrométrie d’absorption atomique en utilisant une simple flamme pour atomiser les éléments. la quantification de cd a` l’état de traces requière donc des méthodes d’analyses sensibles mais aussi exemptes d’interférences. dans ce contexte, le dosage de cd a été réalisé au moyen d’un spectromètre d’absorption atomique couplé a` un générateur d’hydrure en mode continu et combiné a` une méthode de correction du bruit de fond par renversement de spectre. les conditions optimales d’utilisation de la technique ont été déterminées de manière a` obtenir une sensibilité maximale. pour des concentrations de 2,5 et 5 ng ml–1, les absorbances maximales de cd ont été obtenues en préparant une solution de nabh4 a` 3 % dans une solution de soude a` 1,5 % et en analysant des solutions dont la concentration en hno3 a été de 0,3 mol/l. dans ces conditions, la limite de détection a été de 1 ng ml–1 et un coefficient de variation de 5 % a été trouvé en analysant a` 10 reprises des solutions de cd a` 0,2 et 1 ng ml–1, montrant ainsi une bonne sensibilité et reproductibilité de la méthode. de plus, la méthode analytique proposée s’est avérée être une méthode efficace permettant de minimiser les interférences causées par les cations as, al, ca, fe, mn, ni, pb, se et zn jusqu’a` 10 g ml–1. la méthode a été validée par la détermination des concentrations en cd dans les solutions d’extraction au cacl2 0,01 mol/l de sols certifiés et de sols contaminés. les données acquises avec la méthode proposées ont été comparées a` celles obtenues en utilisant des méthodes d’analyses plus conventionnelles, spectrométrie absorption atomique avec atomisation électrothermique et flamme. l’ensemble des résultats obtenus a montré que la méthode proposée était sensible, reproductible et permettait de s’affranchir de certaines interférences. |
Auteurs : Waterlot Christophe; Pelfrêne Aurélie; Douay Francis Effects of iron concentration level in extracting solutions from contaminated soils on the determination of zinc by flame atomic absorption spectrometry with two background correctors Journal of Analytical Methods in Chemistry, pp. 1-101 @article{Waterlot2012d, title = {Effects of iron concentration level in extracting solutions from contaminated soils on the determination of zinc by flame atomic absorption spectrometry with two background correctors}, author = {Christophe Waterlot and Aurélie Pelfrêne and Francis Douay}, year = {2012}, date = {2012-01-01}, journal = {Journal of Analytical Methods in Chemistry}, pages = {1-10}, abstract = {Zinc and iron concentrations were determined after digestion, water, and three-step sequential extractions of contaminated soils. analyses were carried out using flame absorption spectrometry with two background correctors: a deuterium lamp used as the continuum light source (d2 method) and the high-speed self-reversal method (hssr method). regarding the preliminary results obtained with synthetic solutions, the d2 method often emerged as an unsuitable configuration for compensating iron spectral interferences. in contrast, the hssr method appeared as a convenient and powerful configuration and was tested for the determination of zinc in contaminated soils containing high amounts of iron. simple, fast, and interference-free method, the hssr method allows zinc determination at the ppb level in the presence of large amounts of iron with high stability, sensitivity, and reproducibility of results. therefore, the hssr method is described here as a promising approach for monitoring zinc concentrations in various iron-containing samples without any pretreatment}, note = {ACL}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {article} } Zinc and iron concentrations were determined after digestion, water, and three-step sequential extractions of contaminated soils. analyses were carried out using flame absorption spectrometry with two background correctors: a deuterium lamp used as the continuum light source (d2 method) and the high-speed self-reversal method (hssr method). regarding the preliminary results obtained with synthetic solutions, the d2 method often emerged as an unsuitable configuration for compensating iron spectral interferences. in contrast, the hssr method appeared as a convenient and powerful configuration and was tested for the determination of zinc in contaminated soils containing high amounts of iron. simple, fast, and interference-free method, the hssr method allows zinc determination at the ppb level in the presence of large amounts of iron with high stability, sensitivity, and reproducibility of results. therefore, the hssr method is described here as a promising approach for monitoring zinc concentrations in various iron-containing samples without any pretreatment |
Auteurs : Aligon Damien; Douay Francis Site atelier Metaleurop. Synthèse des travaux de recherche réalisés autour de l’ancienne fonderie de Noyelles-Godault @techreport{Aligon2011, title = {Site atelier Metaleurop. Synthèse des travaux de recherche réalisés autour de l’ancienne fonderie de Noyelles-Godault}, author = {Damien Aligon and Francis Douay}, year = {2011}, date = {2011-01-01}, note = {VAL}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {techreport} } |
Auteurs : Baize Denis; Douay Francis; Sterckeman Thibault; Villanneau Estelle; Bourennane Hocine; Ciesielski Henri Teneurs en éléments traces et facteurs d'enrichissement dans les sols agricoles en Nord – Pas-de-Calais Forum GeoReg, 23-27 octobre 2011, Villeneuve d’Ascq @conference{Baize2011, title = {Teneurs en éléments traces et facteurs d'enrichissement dans les sols agricoles en Nord – Pas-de-Calais}, author = {Denis Baize and Francis Douay and Thibault Sterckeman and Estelle Villanneau and Hocine Bourennane and Henri Ciesielski}, year = {2011}, date = {2011-01-01}, booktitle = {Forum GeoReg, 23-27 octobre 2011, Villeneuve d’Ascq}, note = {COM}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } |
Auteurs : Brulle Franck; Lemière Sébastien; Waterlot Christophe; Douay Francis; Vandenbulcke Franck Gene expression analysis of 4 biomarker candidates in Eisenia fetida exposed to an environmental metallic trace elements gradient: A microcosm study Science of the Total Environment, 409(24), pp. 5470-54821 @article{Brulle2011, title = {Gene expression analysis of 4 biomarker candidates in Eisenia fetida exposed to an environmental metallic trace elements gradient: A microcosm study}, author = {Franck Brulle and Sébastien Lemière and Christophe Waterlot and Francis Douay and Franck Vandenbulcke}, year = {2011}, date = {2011-01-01}, journal = {Science of the Total Environment}, volume = {409}, number = {24}, pages = {5470-5482}, abstract = {Past activities of 2 smelters (metaleurop nord and nyrstar) led to the accumulation of high amounts of metal trace elements (tes) in top soils of the noyelles-godault/auby area, northern france. earthworms were exposed to polluted soils collected in this area to study and better understand the physiological changes, the mechanisms of acclimation, and detoxification resulting from te exposure. previously we have cloned and transcriptionally characterized potential biomarkers from immune cells of the ecotoxicologically important earthworm species eisenia fetida exposed in vivo to te-spiked standard soils. in the present study, analysis of expression kinetics of four candidate indicator genes (cadmium-metallothionein, coactosin like protein, phytochelatin synthase and lysenin) was performed in e. fetida after microcosm exposures to natural soils exhibiting an environmental cadmium (cd) gradient in a kinetic manner. te body burdens were also measured. this microcosm study provided insights into: (1) the ability of the 4 tested genes to serve as expression biomarkers, (2) detoxification processes through the expression analysis of selected genes, and (3) influence of land uses on the response of potential biomarkers (gene expression or te uptake). (c) 2011 elsevier b.v. all rights reserved.}, note = {ACL}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {article} } Past activities of 2 smelters (metaleurop nord and nyrstar) led to the accumulation of high amounts of metal trace elements (tes) in top soils of the noyelles-godault/auby area, northern france. earthworms were exposed to polluted soils collected in this area to study and better understand the physiological changes, the mechanisms of acclimation, and detoxification resulting from te exposure. previously we have cloned and transcriptionally characterized potential biomarkers from immune cells of the ecotoxicologically important earthworm species eisenia fetida exposed in vivo to te-spiked standard soils. in the present study, analysis of expression kinetics of four candidate indicator genes (cadmium-metallothionein, coactosin like protein, phytochelatin synthase and lysenin) was performed in e. fetida after microcosm exposures to natural soils exhibiting an environmental cadmium (cd) gradient in a kinetic manner. te body burdens were also measured. this microcosm study provided insights into: (1) the ability of the 4 tested genes to serve as expression biomarkers, (2) detoxification processes through the expression analysis of selected genes, and (3) influence of land uses on the response of potential biomarkers (gene expression or te uptake). (c) 2011 elsevier b.v. all rights reserved. |
Auteurs : Douay Francis L’impact de la pollution sur l’environnement: exemples concrets Renouvellement urbain sur sites et sols pollués: traiter l'héritage du passé pour la ville de demain, essai d'une approche interdisciplinaire. Université Lille 1. 8 décembre 2009 & 11 mai 2010, Villeneuve d'Ascq @conference{Douay2011, title = {L’impact de la pollution sur l’environnement: exemples concrets}, author = {Francis Douay}, year = {2011}, date = {2011-01-01}, booktitle = {Renouvellement urbain sur sites et sols pollués: traiter l'héritage du passé pour la ville de demain, essai d'une approche interdisciplinaire. Université Lille 1. 8 décembre 2009 & 11 mai 2010, Villeneuve d'Ascq}, abstract = {Les activités du laboratoire sols et environnement de l’institut portent sur cinq thématiques. connaître les paramètres physicochimiques des sols régionaux, c'est-à-dire élaborer les bases de données qui renseignent sur ces paramètres des sols. diagnostiquer leur degré de contamination, principalement en éléments métalliques. etudier le comportement de ces polluants dans les sols. contribuer à l’évaluation de l’exposition aux polluants des populations. proposer et évaluer des techniques de remédiation de sols fortement contaminés, principalement en utilisant une végétation, qu’elle soit arborée ou herbacée. l’équipe pluridisciplinaire est constituée de 14 personnes, 7 enseignants chercheurs, 1 ingénieur recherche, 1 ingénieur d’étude, et trois doctorants et post-doctorant. l’intérêt de nos travaux, c’est qu’ils sont menés dans le cadre d’un vaste partenariat universitaire, institutionnel et industriel, et nous avons beaucoup profité du programme de recherche présenté par jean-marie haguenoer qui nous a permis notamment d’accéder à un site atelier exceptionnel. l’impact de la pollution sur l’environnement est une question extrêmement complexe à plusieurs titres. il s’agit de polluants d’origine et de nature très diverses, regroupés en deux grandes catégories : polluants organiques et polluants inorganiques. ils concernent divers compartiments environnementaux : l’eau, l’air, le sol, les poussières, la biosphère, les plantes, les animaux. on est souvent en présence des pollutions des sols à des degrés très variables, mais également multiples, c'est-à-dire combinant à la fois des polluants métalliques et/ou inorganiques. leurs comportements diffèrent selon leur nature, leur degré de contamination et leur forme chimique. enfin, des dangers environnementaux et sanitaires sont très variables et plus ou moins connus selon la concentration et la nature des polluants.}, note = {ACTN}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } Les activités du laboratoire sols et environnement de l’institut portent sur cinq thématiques. connaître les paramètres physicochimiques des sols régionaux, c'est-à-dire élaborer les bases de données qui renseignent sur ces paramètres des sols. diagnostiquer leur degré de contamination, principalement en éléments métalliques. etudier le comportement de ces polluants dans les sols. contribuer à l’évaluation de l’exposition aux polluants des populations. proposer et évaluer des techniques de remédiation de sols fortement contaminés, principalement en utilisant une végétation, qu’elle soit arborée ou herbacée. l’équipe pluridisciplinaire est constituée de 14 personnes, 7 enseignants chercheurs, 1 ingénieur recherche, 1 ingénieur d’étude, et trois doctorants et post-doctorant. l’intérêt de nos travaux, c’est qu’ils sont menés dans le cadre d’un vaste partenariat universitaire, institutionnel et industriel, et nous avons beaucoup profité du programme de recherche présenté par jean-marie haguenoer qui nous a permis notamment d’accéder à un site atelier exceptionnel. l’impact de la pollution sur l’environnement est une question extrêmement complexe à plusieurs titres. il s’agit de polluants d’origine et de nature très diverses, regroupés en deux grandes catégories : polluants organiques et polluants inorganiques. ils concernent divers compartiments environnementaux : l’eau, l’air, le sol, les poussières, la biosphère, les plantes, les animaux. on est souvent en présence des pollutions des sols à des degrés très variables, mais également multiples, c'est-à-dire combinant à la fois des polluants métalliques et/ou inorganiques. leurs comportements diffèrent selon leur nature, leur degré de contamination et leur forme chimique. enfin, des dangers environnementaux et sanitaires sont très variables et plus ou moins connus selon la concentration et la nature des polluants. |
Auteurs : Douay Francis; Leprêtre Alain; Therssen Eric; Deram Annabelle; Shirali A; Cazier Fabrice; Richard Antoine; Scheifler Renaud; Statnik Corine; Muchembled Jérôme; Blarel Jacques; Lefevre B Evaluation d'un mode de gestion durable de sols agricoles fortement contaminés par les éléments traces métalliques : application aux alentours de l’ancienne fonderie de plomb Metaleurop Nord. Forum GeoReg. 23-27 octobre 2011, Villeneuve d’Ascq @conference{Douay2011a, title = {Evaluation d'un mode de gestion durable de sols agricoles fortement contaminés par les éléments traces métalliques : application aux alentours de l’ancienne fonderie de plomb Metaleurop Nord.}, author = {Francis Douay and Alain Leprêtre and Eric Therssen and Annabelle Deram and A Shirali and Fabrice Cazier and Antoine Richard and Renaud Scheifler and Corine Statnik and Jérôme Muchembled and Jacques Blarel and B Lefevre}, year = {2011}, date = {2011-01-01}, booktitle = {Forum GeoReg. 23-27 octobre 2011, Villeneuve d’Ascq}, note = {ACTN}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } |
Auteurs : Douay Francis; Lopareva-Pohu Alena; Pourrut Bertrand; Waterlot Christophe; Garçon Guillaume; Bidar Géraldine; Pruvot Christelle; Shirali Pirouz Impacts d’un mode de phytomanagement sur des sols fortement contaminés par des métaux ValBiom, 8èmes rencontres de la Biomasse. 26 octobre 2011, Gembloux (Belgique) @conference{Douay2011b, title = {Impacts d’un mode de phytomanagement sur des sols fortement contaminés par des métaux}, author = {Francis Douay and Alena Lopareva-Pohu and Bertrand Pourrut and Christophe Waterlot and Guillaume Garçon and Géraldine Bidar and Christelle Pruvot and Pirouz Shirali}, year = {2011}, date = {2011-01-01}, booktitle = {ValBiom, 8èmes rencontres de la Biomasse. 26 octobre 2011, Gembloux (Belgique)}, note = {COM}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } |
Auteurs : Douay Francis; Planque Julie; Loriette Alexandre; Fourrier Hervé Site Metaleurop Nord à Noyelles-Godault (62). Campagnes de mesures des teneurs en plomb et en cadmium autour de l’ancien site industriel @techreport{Douay2011d, title = {Site Metaleurop Nord à Noyelles-Godault (62). Campagnes de mesures des teneurs en plomb et en cadmium autour de l’ancien site industriel}, author = {Francis Douay and Julie Planque and Alexandre Loriette and Hervé Fourrier}, year = {2011}, date = {2011-01-01}, abstract = {Ce présent rapport porte sur la réalisation d’une nouvelle campagne de mesures des concentrations en plomb et en cadmium dans les sols et les productions végétales qu’elles soient agricoles ou potagères dans la zone à plus de 200 mg de plomb kg‐1 autour de l’ancienne fonderie de plomb metaleurop nord (evin‐malmaison, courcelles les lens, noyelles‐godault, dourges, ostricourt, leforest et auby). la démarche vise à conforter ou redéfinir le périmètre du pig (établi en 1999) sur la base de données acquises sept ans après l’arrêt des activités de l’usine. les concentrations en plomb et en cadmium ont été mesurées dans l’horizon organo‐minéral (0 ‐ 25 cm) en 328 points répartis sur l’ensemble du secteur concerné (228 sols agricoles et 100 sols urbains). en parallèle, la teneur en ces deux éléments a été mesurée dans 303 prélèvements de végétaux (95 plantes agricoles et 208 plantes potagères). des semences et plants de variétés identiques ont été distribués aux jardiniers. les végétaux ont été préparés en fonction de leur destination et seules les parties habituellement consommées ont été analysées. la mesure des concentrations totales en pb et cd des sols et des végétaux a été confiée à l’inra. l’analyse statistique des concentrations totales en pb et en cd (campagne 2010 et campagnes antérieures) n’a pas mis en évidence une évolution notable du degré de contamination des sols depuis l’arrêt de l’activité. les sols présentent toujours aujourd’hui des concentrations totales en pb et cd qui dépassent d’une façon significative les teneurs agricoles habituelles régionales. l’analyse géostatistiques réalisée sur l’ensemble des informations acquises depuis l’arrêt des émissions de l’usine (plus de 1 000 données) atteste d’un fort degré de contamination de l’horizon organo‐minéral, d’une contamination plus importante des sols urbains et des habitats ligneux, de l’existence de « hot spots » de contamination (gare d’eau de courcelles les lens, site de stockage des boues de curage de la deûle). en comparaison des travaux antérieurs, l’accroissement du nombre de données analytiques a permis de préciser les courbes d’isoconcentration en pb et cd des sols. au regard de la réglementation en vigueur, la production agricole de végétaux pour l’alimentation humaine reste préoccupante dans la zone où les concentrations excèdent 4 mg de cadmium kg‐1 (grain de blé, d’escourgeon, féverole et betterave sucrière). la plupart des productions sont toutefois conformes pour l’alimentation animale (sauf les pailles de céréales et le maïs fourrager). la production de légumes au niveau des potagers est beaucoup plus problématique, ils sont souvent non‐conformes pour l’alimentation humaine. les plus cultivés (carotte, pomme de terre et poireau) sont d’ailleurs ceux qui présentent les plus forts taux de non‐conformité. de manière générale, la situation est plus préoccupante en ce qui concerne les concentrations totales en cd observées dans les végétaux qu’ils proviennent de l’agriculture ou des potagers. la situation environnementale a peu évolué depuis l’arrêt de l’activité de l’usine. des denrées alimentaires non‐conformes sont actuellement mises sur le marché. le dispositif en place devrait être renforcé pour garantir la sécurité alimentaire tout en assurant l’avenir de l’agriculture dans la zone concernée. les mesures de gestion devraient être étendues à partir de la zone qui présente des concentrations supérieures à 200 mg de pb kg‐1 et/ou à 4 mg de cd kg‐1.pour les sols urbains une stratégie de remédiation des sols selon leurs usages et les dangers pour les populations devrait être mise en place. attendues des populations locales, ces actions devraient être concomitantes d’un engagement des instances régionales, de la volonté et de la concertation des gestionnaires territoriaux.}, note = {VAL}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {techreport} } Ce présent rapport porte sur la réalisation d’une nouvelle campagne de mesures des concentrations en plomb et en cadmium dans les sols et les productions végétales qu’elles soient agricoles ou potagères dans la zone à plus de 200 mg de plomb kg‐1 autour de l’ancienne fonderie de plomb metaleurop nord (evin‐malmaison, courcelles les lens, noyelles‐godault, dourges, ostricourt, leforest et auby). la démarche vise à conforter ou redéfinir le périmètre du pig (établi en 1999) sur la base de données acquises sept ans après l’arrêt des activités de l’usine. les concentrations en plomb et en cadmium ont été mesurées dans l’horizon organo‐minéral (0 ‐ 25 cm) en 328 points répartis sur l’ensemble du secteur concerné (228 sols agricoles et 100 sols urbains). en parallèle, la teneur en ces deux éléments a été mesurée dans 303 prélèvements de végétaux (95 plantes agricoles et 208 plantes potagères). des semences et plants de variétés identiques ont été distribués aux jardiniers. les végétaux ont été préparés en fonction de leur destination et seules les parties habituellement consommées ont été analysées. la mesure des concentrations totales en pb et cd des sols et des végétaux a été confiée à l’inra. l’analyse statistique des concentrations totales en pb et en cd (campagne 2010 et campagnes antérieures) n’a pas mis en évidence une évolution notable du degré de contamination des sols depuis l’arrêt de l’activité. les sols présentent toujours aujourd’hui des concentrations totales en pb et cd qui dépassent d’une façon significative les teneurs agricoles habituelles régionales. l’analyse géostatistiques réalisée sur l’ensemble des informations acquises depuis l’arrêt des émissions de l’usine (plus de 1 000 données) atteste d’un fort degré de contamination de l’horizon organo‐minéral, d’une contamination plus importante des sols urbains et des habitats ligneux, de l’existence de « hot spots » de contamination (gare d’eau de courcelles les lens, site de stockage des boues de curage de la deûle). en comparaison des travaux antérieurs, l’accroissement du nombre de données analytiques a permis de préciser les courbes d’isoconcentration en pb et cd des sols. au regard de la réglementation en vigueur, la production agricole de végétaux pour l’alimentation humaine reste préoccupante dans la zone où les concentrations excèdent 4 mg de cadmium kg‐1 (grain de blé, d’escourgeon, féverole et betterave sucrière). la plupart des productions sont toutefois conformes pour l’alimentation animale (sauf les pailles de céréales et le maïs fourrager). la production de légumes au niveau des potagers est beaucoup plus problématique, ils sont souvent non‐conformes pour l’alimentation humaine. les plus cultivés (carotte, pomme de terre et poireau) sont d’ailleurs ceux qui présentent les plus forts taux de non‐conformité. de manière générale, la situation est plus préoccupante en ce qui concerne les concentrations totales en cd observées dans les végétaux qu’ils proviennent de l’agriculture ou des potagers. la situation environnementale a peu évolué depuis l’arrêt de l’activité de l’usine. des denrées alimentaires non‐conformes sont actuellement mises sur le marché. le dispositif en place devrait être renforcé pour garantir la sécurité alimentaire tout en assurant l’avenir de l’agriculture dans la zone concernée. les mesures de gestion devraient être étendues à partir de la zone qui présente des concentrations supérieures à 200 mg de pb kg‐1 et/ou à 4 mg de cd kg‐1.pour les sols urbains une stratégie de remédiation des sols selon leurs usages et les dangers pour les populations devrait être mise en place. attendues des populations locales, ces actions devraient être concomitantes d’un engagement des instances régionales, de la volonté et de la concertation des gestionnaires territoriaux. |
Auteurs : Douay Francis; Pourrut Bertrand; Waterlot Christophe; Lopareva-Pohu Alena; Pruvot Christelle; Garçon Guillaume; Bidar Géraldine; Shirali Pirouz Fly ash-aided phytostabilisation of highly contaminated soils: assessment of field trial after 8 years 11th International Conference on the Biogeochemistry of Trace Elements (ICOBTE). 3-7 July 2011, Florence (Italie) @conference{Douay2011c, title = {Fly ash-aided phytostabilisation of highly contaminated soils: assessment of field trial after 8 years}, author = {Francis Douay and Bertrand Pourrut and Christophe Waterlot and Alena Lopareva-Pohu and Christelle Pruvot and Guillaume Garçon and Géraldine Bidar and Pirouz Shirali}, year = {2011}, date = {2011-01-01}, booktitle = {11th International Conference on the Biogeochemistry of Trace Elements (ICOBTE). 3-7 July 2011, Florence (Italie)}, note = {ACTI}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } |
Auteurs : Fritsch Clémentine; Coeurdassier Michaël; Giraudoux Patrick; Douay Francis; Rieffel Dominique; De-Vaufleury Annette; Scheifler Renaud Spatially explicit analysis of metal transfer to biota: influence of soil contamination and landscape PLoS ONE, 6(5), pp. e20682 (p 1-16)1 @article{Fritsch2011, title = {Spatially explicit analysis of metal transfer to biota: influence of soil contamination and landscape}, author = {Clémentine Fritsch and Michaël Coeurdassier and Patrick Giraudoux and Francis Douay and Dominique Rieffel and Annette De-Vaufleury and Renaud Scheifler}, year = {2011}, date = {2011-01-01}, journal = {PLoS ONE}, volume = {6}, number = {5}, pages = {e20682 (p 1-16)}, abstract = {Concepts and developments for a new field in ecotoxicology, referred to as ‘‘landscape ecotoxicology,’’ were proposed in the 1990s; however, to date, few studies have been developed in this emergent field. in fact, there is a strong interest in developing this area, both for renewing the concepts and tools used in ecotoxicology as well as for responding to practical issues, such as risk assessment. the aim of this study was to investigate the spatial heterogeneity of metal bioaccumulation in animals in order to identify the role of spatially explicit factors, such as landscape as well as total and extractable metal concentrations in soils. over a smelter-impacted area, we studied the accumulation of trace metals (tms: cd, pb and zn) in invertebrates (the grove snail cepaea sp and the glass snail oxychilus draparnaudi) and vertebrates (the bank vole myodes glareolus and the greater white-toothed shrew crocidura russula). total and cacl2-extractable concentrations of tms were measured in soils from woody patches where the animals were captured. tm concentrations in animals exhibited a high spatial heterogeneity. they increased with soil pollution and were better explained by total rather than cacl2-extractable tm concentrations, except in cepaea sp. tm levels in animals and their variations along the pollution gradient were modulated by the landscape, and this influence was species and metal specific. median soil metal concentrations (predicted by universal kriging) were calculated in buffers of increasing size and were related to bioaccumulation. the spatial scale at which tm concentrations in animals and soils showed the strongest correlations varied between metals, species and landscapes. the potential underlying mechanisms of landscape influence (community functioning, behaviour, etc.) are discussed. present results highlight the need for the further development of landscape ecotoxicology and multi-scale approaches, which would enhance our understanding of pollutant transfer and effects in ecosystems.}, note = {ACLO}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {article} } Concepts and developments for a new field in ecotoxicology, referred to as ‘‘landscape ecotoxicology,’’ were proposed in the 1990s; however, to date, few studies have been developed in this emergent field. in fact, there is a strong interest in developing this area, both for renewing the concepts and tools used in ecotoxicology as well as for responding to practical issues, such as risk assessment. the aim of this study was to investigate the spatial heterogeneity of metal bioaccumulation in animals in order to identify the role of spatially explicit factors, such as landscape as well as total and extractable metal concentrations in soils. over a smelter-impacted area, we studied the accumulation of trace metals (tms: cd, pb and zn) in invertebrates (the grove snail cepaea sp and the glass snail oxychilus draparnaudi) and vertebrates (the bank vole myodes glareolus and the greater white-toothed shrew crocidura russula). total and cacl2-extractable concentrations of tms were measured in soils from woody patches where the animals were captured. tm concentrations in animals exhibited a high spatial heterogeneity. they increased with soil pollution and were better explained by total rather than cacl2-extractable tm concentrations, except in cepaea sp. tm levels in animals and their variations along the pollution gradient were modulated by the landscape, and this influence was species and metal specific. median soil metal concentrations (predicted by universal kriging) were calculated in buffers of increasing size and were related to bioaccumulation. the spatial scale at which tm concentrations in animals and soils showed the strongest correlations varied between metals, species and landscapes. the potential underlying mechanisms of landscape influence (community functioning, behaviour, etc.) are discussed. present results highlight the need for the further development of landscape ecotoxicology and multi-scale approaches, which would enhance our understanding of pollutant transfer and effects in ecosystems. |
Auteurs : Godet Jean-Philippe; Demuynck Sylvain; Waterlot Christophe; Lemière Sébastien; Souty-Grosset Catherine; Scheifler Renaud; Douay Francis; Leprêtre Alain; Pruvot Christelle Growth and metal accumulation in Porcellio scaber exposed to poplar litter from Cd-, Pb- and Zn-contaminated sites Ecotoxicology and Environmental Safety, 74(3), pp. 451-4581 @article{Godet2011, title = {Growth and metal accumulation in Porcellio scaber exposed to poplar litter from Cd-, Pb- and Zn-contaminated sites}, author = {Jean-Philippe Godet and Sylvain Demuynck and Christophe Waterlot and Sébastien Lemière and Catherine Souty-Grosset and Renaud Scheifler and Francis Douay and Alain Leprêtre and Christelle Pruvot}, year = {2011}, date = {2011-01-01}, journal = {Ecotoxicology and Environmental Safety}, volume = {74}, number = {3}, pages = {451-458}, abstract = {This study aimed at determining, in the laboratory, the effects of poplar litter collected in woody habitats contaminated by heavy metals on growth and metal accumulation in the terrestrial isopod porcellio scaber. cd, pb, and zn pseudototal and cacl2-extractable concentrations in litter types were determined using aas. juveniles were fed ad libitum, individually, for 28 days with four litter types presenting an increasing gradient of metal contamination. individuals were weighed every week and metal body burdens were determined at the end of the experiment. from the first week until the end of the experiment, a decrease in p. scaber growth related to the increase of metal concentration in litter types was recorded. significant correlations were observed between metal body burdens and metal concentrations in litter types. however, cd accumulation in woodlice appeared to be related to the cd/zn concentration ratio in litter types. all these results showed the potential of weight gain in p. scaber as a suitable indicator for litter quality assessment with ecological relevance.}, note = {ACL}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {article} } This study aimed at determining, in the laboratory, the effects of poplar litter collected in woody habitats contaminated by heavy metals on growth and metal accumulation in the terrestrial isopod porcellio scaber. cd, pb, and zn pseudototal and cacl2-extractable concentrations in litter types were determined using aas. juveniles were fed ad libitum, individually, for 28 days with four litter types presenting an increasing gradient of metal contamination. individuals were weighed every week and metal body burdens were determined at the end of the experiment. from the first week until the end of the experiment, a decrease in p. scaber growth related to the increase of metal concentration in litter types was recorded. significant correlations were observed between metal body burdens and metal concentrations in litter types. however, cd accumulation in woodlice appeared to be related to the cd/zn concentration ratio in litter types. all these results showed the potential of weight gain in p. scaber as a suitable indicator for litter quality assessment with ecological relevance. |
Auteurs : Lafeuille Christine; Mossmann Jean-Rémi; Haguenoer Jean-Marie; Douay Francis Table ronde sur le renouvellement urbain face aux impacts sanitaires et environnementaux Renouvellement urbain sur sites et sols pollués: traiter l'héritage du passé pour la ville de demain, essai d'une approche interdisciplinaire.Université Lille 1. 8 décembre 2009 & 11 mai 2010, Villeneuve d'Ascq @conference{Lafeuille2011, title = {Table ronde sur le renouvellement urbain face aux impacts sanitaires et environnementaux}, author = {Christine Lafeuille and Jean-Rémi Mossmann and Jean-Marie Haguenoer and Francis Douay}, year = {2011}, date = {2011-01-01}, booktitle = {Renouvellement urbain sur sites et sols pollués: traiter l'héritage du passé pour la ville de demain, essai d'une approche interdisciplinaire.Université Lille 1. 8 décembre 2009 & 11 mai 2010, Villeneuve d'Ascq}, note = {ACTN}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } |
Auteurs : Lopareva-Pohu Alena; Pourrut Bertrand; Waterlot Christophe; Garçon Guillaume; Bidar Géraldine; Pruvot Christelle; Shirali Pirouz; Douay Francis Assessment of fly ash-aided phytostabilisation of highly contaminated soils after an 8-year field trial Part 1. Influence on soil parameters and metal extractability Science of the Total Environment, 409(3), pp. 647-6541 @article{Lopareva-Pohu2011b, title = {Assessment of fly ash-aided phytostabilisation of highly contaminated soils after an 8-year field trial Part 1. Influence on soil parameters and metal extractability}, author = {Alena Lopareva-Pohu and Bertrand Pourrut and Christophe Waterlot and Guillaume Garçon and Géraldine Bidar and Christelle Pruvot and Pirouz Shirali and Francis Douay}, year = {2011}, date = {2011-01-01}, journal = {Science of the Total Environment}, volume = {409}, number = {3}, pages = {647-654}, abstract = {Sustainable management of large surface areas contaminated with trace elements is a real challenge, since currently applied remediation techniques are too expensive for these areas. aided phytostabilisation appears to be a cost efficient technique to reduce metal mobility in contaminated soils and contaminated particle spread. in this context, this study aimed at evaluating the long-term efficiency of aided phytostabilisation on former agricultural soils highly contaminated with trace elements. the influence of afforestation and fly ash amendments to reduce metal mobility was investigated. before being planted with a tree mix, the study site was divided into three plots: a reference plot with no amendment, the second amended with silico-aluminous fly ash and the third with sulfo-calcic fly ash. after eight years, some soil physico-chemical parameters, including cadmium (cd), lead (pb) and zinc (zn) extractability were modified. in particular, ph decreased on the whole site while organic carbon content increased. the alteration of these parameters influencing trace element mobility is explained by afforestation. over time, concentrations of cacl2-extractable metals increased and were correlated with the soil ph decrease. in the amended soils, extractable cd, pb and zn concentrations were lower than in the reference soil. the results indicated that the two fly ashes buffered natural soil acidification due to vegetation development and limited trace element mobility and thus could limit their bioavailability. for long-term phytostabilisation, special attention should be focused on the soil ph, metal mobility and phytoavailability analysis. (c) 2010 elsevier b.v. all rights reserved.}, note = {ACL}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {article} } Sustainable management of large surface areas contaminated with trace elements is a real challenge, since currently applied remediation techniques are too expensive for these areas. aided phytostabilisation appears to be a cost efficient technique to reduce metal mobility in contaminated soils and contaminated particle spread. in this context, this study aimed at evaluating the long-term efficiency of aided phytostabilisation on former agricultural soils highly contaminated with trace elements. the influence of afforestation and fly ash amendments to reduce metal mobility was investigated. before being planted with a tree mix, the study site was divided into three plots: a reference plot with no amendment, the second amended with silico-aluminous fly ash and the third with sulfo-calcic fly ash. after eight years, some soil physico-chemical parameters, including cadmium (cd), lead (pb) and zinc (zn) extractability were modified. in particular, ph decreased on the whole site while organic carbon content increased. the alteration of these parameters influencing trace element mobility is explained by afforestation. over time, concentrations of cacl2-extractable metals increased and were correlated with the soil ph decrease. in the amended soils, extractable cd, pb and zn concentrations were lower than in the reference soil. the results indicated that the two fly ashes buffered natural soil acidification due to vegetation development and limited trace element mobility and thus could limit their bioavailability. for long-term phytostabilisation, special attention should be focused on the soil ph, metal mobility and phytoavailability analysis. (c) 2010 elsevier b.v. all rights reserved. |
Auteurs : Lopareva-Pohu Alena; Verdin Anthony; Garçon Guillaume; Lounès-Hadj-Sahraoui Anissa; Pourrut Bertrand; Debiane Djouher; Waterlot Christophe; Laruelle Frédéric; Bidar Géraldine; Douay Francis; Shirali Pirouz Influence of fly ash aided phytostabilisation of Pb, Cd and Zn highly contaminated soils on Lolium perenne and Trifolium repens metal transfer and physiological stress Environmental Pollution, 159(6), pp. 1721-17291 @article{Lopareva-Pohu2011a, title = {Influence of fly ash aided phytostabilisation of Pb, Cd and Zn highly contaminated soils on Lolium perenne and Trifolium repens metal transfer and physiological stress}, author = {Alena Lopareva-Pohu and Anthony Verdin and Guillaume Garçon and Anissa Lounès-Hadj-Sahraoui and Bertrand Pourrut and Djouher Debiane and Christophe Waterlot and Frédéric Laruelle and Géraldine Bidar and Francis Douay and Pirouz Shirali}, year = {2011}, date = {2011-01-01}, journal = {Environmental Pollution}, volume = {159}, number = {6}, pages = {1721-1729}, abstract = {Due to anthropogenic activities, large extends of soils are highly contaminated by metal trace element (mte). aided phytostabilisation aims to establish a vegetation cover in order to promote in situ immobilisation of trace elements by combining the use of metal-tolerant plants and inexpensive mineral or organic soil amendments. eight years after coal fly ash (cfa) soil amendment, mte bioavailability and uptake by two plants, lolium perenne and trifolium repens, were evaluated, as some biological markers reflecting physiological stress. results showed that the two plant species under study were suitable to reduce the mobility and the availability of these elements. moreover, the plant growth was better on cfa amended mte-contaminated soils, and the plant sensitivity to mte-induced physiological stress, as studied through photosynthetic pigment contents and oxidative damage was lower or similar. in conclusion, these results supported the usefulness of aided phytostabilisation of mte-highly contaminated soils. highlights ► aided phytostabilisation aims to establish a vegetation cover in order to promote immobilisation of mte. ► 8 years after the soil amendments, a pot culture study was carried out in greenhouse conditions. ► mte bioavailability and uptake by the two plants was drastically decreased with amendments. ► our results support the usefulness of aided phytostabilisation of mte-highly contaminated soils. ► cfa addition contributed to the reduction of the mte mobility and availability for the plants}, note = {ACL}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {article} } Due to anthropogenic activities, large extends of soils are highly contaminated by metal trace element (mte). aided phytostabilisation aims to establish a vegetation cover in order to promote in situ immobilisation of trace elements by combining the use of metal-tolerant plants and inexpensive mineral or organic soil amendments. eight years after coal fly ash (cfa) soil amendment, mte bioavailability and uptake by two plants, lolium perenne and trifolium repens, were evaluated, as some biological markers reflecting physiological stress. results showed that the two plant species under study were suitable to reduce the mobility and the availability of these elements. moreover, the plant growth was better on cfa amended mte-contaminated soils, and the plant sensitivity to mte-induced physiological stress, as studied through photosynthetic pigment contents and oxidative damage was lower or similar. in conclusion, these results supported the usefulness of aided phytostabilisation of mte-highly contaminated soils. highlights ► aided phytostabilisation aims to establish a vegetation cover in order to promote immobilisation of mte. ► 8 years after the soil amendments, a pot culture study was carried out in greenhouse conditions. ► mte bioavailability and uptake by the two plants was drastically decreased with amendments. ► our results support the usefulness of aided phytostabilisation of mte-highly contaminated soils. ► cfa addition contributed to the reduction of the mte mobility and availability for the plants |
Auteurs : Lucas Jean-Paul; Le-Bot Barbara; Glorennec Philippe; Etchevers Anne; Bretin Philippe; Douay Francis; Sébille Véronique; Bellanger Lise; Mandin Corinne Lead Contamination in French housing International Society of Exposure Science. 23-27 October 2011, Baltimore (USA) @conference{Lucas2011, title = {Lead Contamination in French housing}, author = {Jean-Paul Lucas and Barbara Le-Bot and Philippe Glorennec and Anne Etchevers and Philippe Bretin and Francis Douay and Véronique Sébille and Lise Bellanger and Corinne Mandin}, year = {2011}, date = {2011-01-01}, booktitle = {International Society of Exposure Science. 23-27 October 2011, Baltimore (USA)}, abstract = {White lead was extensively used from the 18th century in europe. since the beginning of the 20th century, french laws have served to reduce the use of lead in paints. since 1999, mandatory controls have been screening for the presence of lead in french housing, but their data are not centralized. the aim of this study is to describe the lead contamination in the dwellings of children aged 6 months to 6 years in metropolitan france. total and leachable lead levels were measured in settled dust, outdoor playgrounds and paint chips, and dissolved lead was measured in tap water of 484 dwellings. the statistical analysis took into account the sampling design and the adjusted sampling weights. less than 1% of dwellings have a lead concentration ≥ 25μg/l in tap water; 0.25% has an average lead content ≥ 40μg/ft2 in floor dust; 1.3% of outdoor playground top soils have a lead concentration ≥ 400mg/kg. concentrations depend on the age of building and on the urban/rural location. lead-based paints (≥1mg/cm2) are more prevalent in the oldest homes (built before 1949, the year that leaded paint was supposed not to be used anymore). in homes built between 1949 and 1975, surfaces coated with white lead-based paints are rare; in units built from 1975, such surfaces are almost non-existent. however, settled dusts in dwellings built from 1975 to 1993 are as contaminated as those from homes built before 1949. common areas are more contaminated than inside homes. despite the public policies to reduce lead poisoning implemented in the last fifteen years, lead exposure standards do not exist in france, except for drinking water. the prevalence of homes with levels above american regulatory limits is relatively low for each studied media. however, only main residences with children were studied here, whereas other indoor environments may also expose children to lead. this work is continuing, with the objective of providing tools to identify high risk housing in france.}, note = {COM}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } White lead was extensively used from the 18th century in europe. since the beginning of the 20th century, french laws have served to reduce the use of lead in paints. since 1999, mandatory controls have been screening for the presence of lead in french housing, but their data are not centralized. the aim of this study is to describe the lead contamination in the dwellings of children aged 6 months to 6 years in metropolitan france. total and leachable lead levels were measured in settled dust, outdoor playgrounds and paint chips, and dissolved lead was measured in tap water of 484 dwellings. the statistical analysis took into account the sampling design and the adjusted sampling weights. less than 1% of dwellings have a lead concentration ≥ 25μg/l in tap water; 0.25% has an average lead content ≥ 40μg/ft2 in floor dust; 1.3% of outdoor playground top soils have a lead concentration ≥ 400mg/kg. concentrations depend on the age of building and on the urban/rural location. lead-based paints (≥1mg/cm2) are more prevalent in the oldest homes (built before 1949, the year that leaded paint was supposed not to be used anymore). in homes built between 1949 and 1975, surfaces coated with white lead-based paints are rare; in units built from 1975, such surfaces are almost non-existent. however, settled dusts in dwellings built from 1975 to 1993 are as contaminated as those from homes built before 1949. common areas are more contaminated than inside homes. despite the public policies to reduce lead poisoning implemented in the last fifteen years, lead exposure standards do not exist in france, except for drinking water. the prevalence of homes with levels above american regulatory limits is relatively low for each studied media. however, only main residences with children were studied here, whereas other indoor environments may also expose children to lead. this work is continuing, with the objective of providing tools to identify high risk housing in france. |
Auteurs : Pelfrêne Aurélie; Waterlot Christophe; Douay Francis In vitro digestion and DGT techniques for estimating cadmium and lead bioavailability in contaminated soils: Influence of gastric juice pH Science of the Total Environment, 409, pp. 5076-50851 @article{Pelfrene2011, title = {In vitro digestion and DGT techniques for estimating cadmium and lead bioavailability in contaminated soils: Influence of gastric juice pH}, author = {Aurélie Pelfrêne and Christophe Waterlot and Francis Douay}, year = {2011}, date = {2011-01-01}, journal = {Science of the Total Environment}, volume = {409}, pages = {5076-5085}, abstract = {A sensitivity analysis was conducted on an in vitro gastrointestinal digestion test (i) to investigate the influence of a low variation of gastric juice ph on the bioaccessibility of cd and pb in smelter-contaminated soils (fb, using the unified bioaccessibility method ubm) and fractions of metals that may be transported across the intestinal epithelium (fa, using the diffusive gradient in thin film technique), and (ii) to provide a better understanding of the significance of ph in health risk assessment through ingestion of soil by children. the risk of metal exposure to children (hazard quotient, hq) was determined for conditions that represent a worst-case scenario (i.e., ingestion rate of 200 mg day−1) using three separate calculations of metal daily intake: estimated daily intake (edi), bioaccessible edi (edi-fb), and oral bioavailable edi (edi-fa). the increasing ph from 1.2 to 1.7 resulted in: (i) no significant variation in cd-fb in the gastric phase but a decrease in the gastrointestinal phase; (ii) a decrease in soluble pb in the gastric phase and a significant variation in pb-fb in the gastrointestinal phase; (iii) a significant decrease in cd-fa and no variation in pb-fa; (iv) no change in edi-fb and edi-fa hqs for cd; (v) a significant decrease in edi-fb hqs and no significant variation in edi-fa hq for pb. in the analytical conditions, these results show that risk to children decreases when the bioavailability of pb in soils is taken into account and that the studied ph values do not affect the edi-fa hqs. the present results provide evidence that the inclusion of bioavailability analysis during health risk assessment could provide a more realistic estimate of cd and pb exposure, and opens a wide field of practical research on this topic (e.g., in contaminated site management).}, note = {ACL}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {article} } A sensitivity analysis was conducted on an in vitro gastrointestinal digestion test (i) to investigate the influence of a low variation of gastric juice ph on the bioaccessibility of cd and pb in smelter-contaminated soils (fb, using the unified bioaccessibility method ubm) and fractions of metals that may be transported across the intestinal epithelium (fa, using the diffusive gradient in thin film technique), and (ii) to provide a better understanding of the significance of ph in health risk assessment through ingestion of soil by children. the risk of metal exposure to children (hazard quotient, hq) was determined for conditions that represent a worst-case scenario (i.e., ingestion rate of 200 mg day−1) using three separate calculations of metal daily intake: estimated daily intake (edi), bioaccessible edi (edi-fb), and oral bioavailable edi (edi-fa). the increasing ph from 1.2 to 1.7 resulted in: (i) no significant variation in cd-fb in the gastric phase but a decrease in the gastrointestinal phase; (ii) a decrease in soluble pb in the gastric phase and a significant variation in pb-fb in the gastrointestinal phase; (iii) a significant decrease in cd-fa and no variation in pb-fa; (iv) no change in edi-fb and edi-fa hqs for cd; (v) a significant decrease in edi-fb hqs and no significant variation in edi-fa hq for pb. in the analytical conditions, these results show that risk to children decreases when the bioavailability of pb in soils is taken into account and that the studied ph values do not affect the edi-fa hqs. the present results provide evidence that the inclusion of bioavailability analysis during health risk assessment could provide a more realistic estimate of cd and pb exposure, and opens a wide field of practical research on this topic (e.g., in contaminated site management). |
Auteurs : Pelfrêne Aurélie; Waterlot Christophe; Douay Francis Investigation of DGT as a metal speciation tool in artificial human gastrointestinal fluids Analytica Chimica Acta, 699, pp. 177-1861 @article{Pelfrene2011a, title = {Investigation of DGT as a metal speciation tool in artificial human gastrointestinal fluids}, author = {Aurélie Pelfrêne and Christophe Waterlot and Francis Douay}, year = {2011}, date = {2011-01-01}, journal = {Analytica Chimica Acta}, volume = {699}, pages = {177-186}, abstract = {This paper reports the results of an investigation on the performance of the diffusive gradient in thin film technique (dgt) as a speciation tool for trace elements (tes) in artificial human gastrointestinal fluids. the validity of cd, pb, and zn sampling by dgt in digestive fluids was checked. the te bioaccessibility in highly contaminated soils was determined using the in vitro unified barge method (ubm) test. dgt devices were deployed in the gastrointestinal solutions obtained after carrying out the ubm test. the computer speciation code jess (joint expert speciation system) was used to predict the metal speciation of cd, pb, and zn. combining the in vitro test with the dgt technique and jess provided an approach to the te species available for transport across the intestinal epithelium. the gastrointestinal absorption of ingested te ranged from 8 to 30% for cd, 0.6 to 11% for pb, and 0.8 to 7% for zn and was influenced by te speciation. in this original approach, the dgt technique was found to be simple and reliable in the investigation of te chemical speciation in digestive fluids. extrapolation to the in vivo situation should be undertaken very cautiously and requires further investigation.}, note = {ACL}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {article} } This paper reports the results of an investigation on the performance of the diffusive gradient in thin film technique (dgt) as a speciation tool for trace elements (tes) in artificial human gastrointestinal fluids. the validity of cd, pb, and zn sampling by dgt in digestive fluids was checked. the te bioaccessibility in highly contaminated soils was determined using the in vitro unified barge method (ubm) test. dgt devices were deployed in the gastrointestinal solutions obtained after carrying out the ubm test. the computer speciation code jess (joint expert speciation system) was used to predict the metal speciation of cd, pb, and zn. combining the in vitro test with the dgt technique and jess provided an approach to the te species available for transport across the intestinal epithelium. the gastrointestinal absorption of ingested te ranged from 8 to 30% for cd, 0.6 to 11% for pb, and 0.8 to 7% for zn and was influenced by te speciation. in this original approach, the dgt technique was found to be simple and reliable in the investigation of te chemical speciation in digestive fluids. extrapolation to the in vivo situation should be undertaken very cautiously and requires further investigation. |
Auteurs : Pelfrêne Aurélie; Waterlot Christophe; Mazzuca Muriel; Nisse Catherine; Bidar Géraldine; Douay Francis Assessing Cd, Pb, Zn mobility and human bioaccessibility in smelter-contaminated agricultural topsoils (northern France) Environmental Geochemistry and Health, 33(5), pp. 477-4931 @article{Pelfrene2011b, title = {Assessing Cd, Pb, Zn mobility and human bioaccessibility in smelter-contaminated agricultural topsoils (northern France)}, author = {Aurélie Pelfrêne and Christophe Waterlot and Muriel Mazzuca and Catherine Nisse and Géraldine Bidar and Francis Douay}, year = {2011}, date = {2011-01-01}, journal = {Environmental Geochemistry and Health}, volume = {33}, number = {5}, pages = {477-493}, abstract = {The extractability of cd, pb, and zn was investigated in contaminated agricultural topsoils located in an area highly affected by the past atmospheric emissions of two smelters in northern france in order to assess their mobility and human bioaccessibility. the determination of cd, pb, and zn bioaccessibility (unified barge method, in vitro test) was made to evaluate the absolute trace element (te) bioavailability. the results highlighted differences in bioaccessibility between cd, pb, and zn (cd[pb[ zn). the mean values of the bioaccessible fractions of cd, pb, and zn during the gastric phase were 82, 55, and 33%, respectively, of the pseudototal concentrations, whereas during the gastrointestinal phase, the bioaccessible fractions of metals decreased to 45, 20, and 10%, respectively. stepwise multiple regression analysis showed that human bioaccessibility was affected by various physicochemical parameters (i.e., sand, carbonates, organic matter, assimilated p, free al oxides, and pseudototal fe contents). sequential extractions were performed as an indication of the te availability in these soils. cadmium occurred in the more available fractions, pb was mostly present as bound by oxides, and a significant contribution to the pseudototal zn concentration was defined as the unavailable residual form related to the crystalline structures of minerals. the concepts of bioavailability and bioaccessibility are important for quantifying the risks associated with exposure to environmental pollutants and providing more realistic information for human health}, note = {ACL}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {article} } The extractability of cd, pb, and zn was investigated in contaminated agricultural topsoils located in an area highly affected by the past atmospheric emissions of two smelters in northern france in order to assess their mobility and human bioaccessibility. the determination of cd, pb, and zn bioaccessibility (unified barge method, in vitro test) was made to evaluate the absolute trace element (te) bioavailability. the results highlighted differences in bioaccessibility between cd, pb, and zn (cd[pb[ zn). the mean values of the bioaccessible fractions of cd, pb, and zn during the gastric phase were 82, 55, and 33%, respectively, of the pseudototal concentrations, whereas during the gastrointestinal phase, the bioaccessible fractions of metals decreased to 45, 20, and 10%, respectively. stepwise multiple regression analysis showed that human bioaccessibility was affected by various physicochemical parameters (i.e., sand, carbonates, organic matter, assimilated p, free al oxides, and pseudototal fe contents). sequential extractions were performed as an indication of the te availability in these soils. cadmium occurred in the more available fractions, pb was mostly present as bound by oxides, and a significant contribution to the pseudototal zn concentration was defined as the unavailable residual form related to the crystalline structures of minerals. the concepts of bioavailability and bioaccessibility are important for quantifying the risks associated with exposure to environmental pollutants and providing more realistic information for human health |
Auteurs : Pelfrêne Aurélie; Waterlot Christophe; Nisse Catherine; Mazzuca Muriel; Douay Francis Assessing Cd and Pb human bioaccessibility in smelter-contaminated agricultural and urban soils (northern France) 11th International Conference on the Biogeochemistry of Trace Elements (ICOBTE). 3-7 July 2011, Florence (Italy) @conference{Pelfrene2011c, title = {Assessing Cd and Pb human bioaccessibility in smelter-contaminated agricultural and urban soils (northern France)}, author = {Aurélie Pelfrêne and Christophe Waterlot and Catherine Nisse and Muriel Mazzuca and Francis Douay}, year = {2011}, date = {2011-01-01}, booktitle = {11th International Conference on the Biogeochemistry of Trace Elements (ICOBTE). 3-7 July 2011, Florence (Italy)}, note = {ACTI}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } |
Auteurs : Pérès Guénola; Bispo Antonio; Grand Cécile; Gattin Isabelle; Hedde Mickaël; Harris-Hellal Jennifer; Le-Guédard Marina; Ruiz Nuria; Alaphilippe A; Béguiristain Thierry; Douay Francis; Faure Olivier; Hitmi Adnane; Houot Sabine; Legras Marc; Guernion Muriel; Vian Jean-François; Rougé Laurence; Dur Jeanne-Chantal; Cluzeau Daniel Selection of soil bioindicators for impact assessment of land use changes and soil protection. Exemple of the "Bioindicators Programme." The Wageningen Conference on Applied Soil Science. 18-22 September 2011, Wageningen (the Netherlands) @conference{Peres2011, title = {Selection of soil bioindicators for impact assessment of land use changes and soil protection. Exemple of the "Bioindicators Programme."}, author = {Guénola Pérès and Antonio Bispo and Cécile Grand and Isabelle Gattin and Mickaël Hedde and Jennifer Harris-Hellal and Marina Le-Guédard and Nuria Ruiz and A Alaphilippe and Thierry Béguiristain and Francis Douay and Olivier Faure and Adnane Hitmi and Sabine Houot and Marc Legras and Muriel Guernion and Jean-François Vian and Laurence Rougé and Jeanne-Chantal Dur and Daniel Cluzeau}, year = {2011}, date = {2011-01-01}, booktitle = {The Wageningen Conference on Applied Soil Science. 18-22 September 2011, Wageningen (the Netherlands)}, note = {COM}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } |
Auteurs : Pourrut Bertrand; Leprêtre Alain; Therssen Eric; Deram Annabelle; Shirali Pirouz; Cazier Fabrice; Richard Antoine; Scheifler Renaud; Blarel Jacques; Douay Francis; the-PHYTENER-Team The PHYTENER project: development of phytostabilisation combined with energy crop production on agricultural soils highly contaminated by metals European Geosciences Union General Assembly. 3-8 April 2011, Vienna (Austria)13, @conference{Pourrut2011c, title = {The PHYTENER project: development of phytostabilisation combined with energy crop production on agricultural soils highly contaminated by metals}, author = {Bertrand Pourrut and Alain Leprêtre and Eric Therssen and Annabelle Deram and Pirouz Shirali and Fabrice Cazier and Antoine Richard and Renaud Scheifler and Jacques Blarel and Francis Douay and the-PHYTENER-Team}, year = {2011}, date = {2011-01-01}, booktitle = {European Geosciences Union General Assembly. 3-8 April 2011, Vienna (Austria)}, volume = {13}, pages = {1-2}, note = {ACTI}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } |
Auteurs : Pourrut Bertrand; Lopareva-Pohu Alena; Pruvot Christelle; Garçon Guillaume; Verdin Anthony; Waterlot Christophe; Bidar Géraldine; Shirali Pirouz; Douay Francis Assessment of fly ash-aided phytostabilisation of highly contaminated soils after an 8-year field trial. Part 2. Influence on plants Science of the Total Environment, 409, pp. 4504-45101 @article{Pourrut2011a, title = {Assessment of fly ash-aided phytostabilisation of highly contaminated soils after an 8-year field trial. Part 2. Influence on plants}, author = {Bertrand Pourrut and Alena Lopareva-Pohu and Christelle Pruvot and Guillaume Garçon and Anthony Verdin and Christophe Waterlot and Géraldine Bidar and Pirouz Shirali and Francis Douay}, year = {2011}, date = {2011-01-01}, journal = {Science of the Total Environment}, volume = {409}, pages = {4504-4510}, abstract = {Aided phytostabilisation is a cost-efficient technique to manage metal-contaminated areas, particularly in the presence of extensive pollution. plant establishment and survival in highly metal-contaminated soils are crucial for phytostabilisation success, as metal toxicity for plants is widely reported. a relevant phytostabilisation solution must limit metal transfer through the food chain. therefore, this study aimed at evaluating the long-term efficiency of aided phytostabilisation on former agricultural soils highly contaminated by cadmium, lead, and zinc. the influence of afforestation and fly ash amendments on reducing metal phytoavailability was investigated as were their effects on plant development. before being planted with a tree mix, the site was divided into three plots: a reference plot with no amendment, a plot amended with silico-aluminous fly ash and one with sulfo-calcic fly ash. unlike salix alba and quercus robur, alnus glutinosa, acer pseudoplatanus and robinia pseudoacacia grew well on the site and accumulated, overall, quite low concentrations of metals in their leaves and young twigs. this suggests that these three species have an excluder phenotype for cd, zn and pb. after 8 years, metal availability to a. glutinosa, a. pseudoplatanus and r. pseudoacacia, and translocation to their above-ground parts, strongly decreased in fly ash-amended soils. such decreases fit well together with the depletion of cacl2-extractable metals in amended soils. although both fly ashes were effective to decrease cd, pb and zn concentrations in above-ground parts of trees, the sulfo-calcic ash was more efficient.}, note = {ACL}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {article} } Aided phytostabilisation is a cost-efficient technique to manage metal-contaminated areas, particularly in the presence of extensive pollution. plant establishment and survival in highly metal-contaminated soils are crucial for phytostabilisation success, as metal toxicity for plants is widely reported. a relevant phytostabilisation solution must limit metal transfer through the food chain. therefore, this study aimed at evaluating the long-term efficiency of aided phytostabilisation on former agricultural soils highly contaminated by cadmium, lead, and zinc. the influence of afforestation and fly ash amendments on reducing metal phytoavailability was investigated as were their effects on plant development. before being planted with a tree mix, the site was divided into three plots: a reference plot with no amendment, a plot amended with silico-aluminous fly ash and one with sulfo-calcic fly ash. unlike salix alba and quercus robur, alnus glutinosa, acer pseudoplatanus and robinia pseudoacacia grew well on the site and accumulated, overall, quite low concentrations of metals in their leaves and young twigs. this suggests that these three species have an excluder phenotype for cd, zn and pb. after 8 years, metal availability to a. glutinosa, a. pseudoplatanus and r. pseudoacacia, and translocation to their above-ground parts, strongly decreased in fly ash-amended soils. such decreases fit well together with the depletion of cacl2-extractable metals in amended soils. although both fly ashes were effective to decrease cd, pb and zn concentrations in above-ground parts of trees, the sulfo-calcic ash was more efficient. |
Auteurs : Pourrut Bertrand; Pinelli Eric; Silvestre Jérôme; Douay Francis Recommendations for conducting the alkaline comet assay in higher plants 9th International Comet Assay Workshop (ICAW). 13-16 September 2011, Kusadası (Turquie) @conference{Pourrut2011d, title = {Recommendations for conducting the alkaline comet assay in higher plants}, author = {Bertrand Pourrut and Eric Pinelli and Jérôme Silvestre and Francis Douay}, year = {2011}, date = {2011-01-01}, booktitle = {9th International Comet Assay Workshop (ICAW). 13-16 September 2011, Kusadası (Turquie)}, note = {ACTI}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } |
Auteurs : Pruvot Christelle; Godet Jean-Philippe; Demuynck Sylvain; Souty-Grosset Catherine; Douay Francis; Leprêtre Alain The use of fluctuating asymmetry analysis to evaluate the effects of metal-pollution (Cd, Pb, Zn) on Porcellio scaber (Crustacea, Isopoda) living in contaminated woody habitats 12th European Ecological Federation Congress. 25-29 September 2011, Avila (Espagne) @conference{Pruvot2011, title = {The use of fluctuating asymmetry analysis to evaluate the effects of metal-pollution (Cd, Pb, Zn) on Porcellio scaber (Crustacea, Isopoda) living in contaminated woody habitats}, author = {Christelle Pruvot and Jean-Philippe Godet and Sylvain Demuynck and Catherine Souty-Grosset and Francis Douay and Alain Leprêtre}, year = {2011}, date = {2011-01-01}, booktitle = {12th European Ecological Federation Congress. 25-29 September 2011, Avila (Espagne)}, note = {ACTI}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } |
Auteurs : Schwartz Christophe; Chenot Elodie-Denise; Cortet Jérôme; Debeljak Marko; Džeroski Saso; Douay Francis; Dumat Camille; Kocev Dragi; Pernin Céline; Pourrut Bertrand Which strategy for a survey of garden soils? SUITMA 6. 3-7 October 2011, Marrakech (Maroc) @conference{Schwartz2011, title = {Which strategy for a survey of garden soils?}, author = {Christophe Schwartz and Elodie-Denise Chenot and Jérôme Cortet and Marko Debeljak and Saso Džeroski and Francis Douay and Camille Dumat and Dragi Kocev and Céline Pernin and Bertrand Pourrut}, year = {2011}, date = {2011-01-01}, booktitle = {SUITMA 6. 3-7 October 2011, Marrakech (Maroc)}, abstract = {In france and all over the world, there is no systematic data available on the quality (fertility and contamination) of garden soils. nevertheless, there is a growing need for a typology and for a method dedicated to national and international garden soil survey. this inventory is much needed in the context of environmental risk assessment, to predict the potential impact on human health of the direct contact with garden soils and of the consumption of vegetables from gardens. the state of the art on the international knowledge on garden soils, gardening practices and food production, shows that gardens remain poorly known and very complex ecological, economical and social systems. their global quality is the result of a wide number of factors including environment, history, specific characteristics of the gardens, gardeners and their practices, plant and/or animal productions and socio-economic context. the aim is then to better know the determinism of the agronomic, environmental and sanitary properties of gardens as a function of gardening practices and their impact on the quality of soils and plants. we propose a definition of “garden” and more generally of all the field “garden”. the system “garden” is represented by attributes (soil and plant characteristics) and factors with various impacts (e.g. environment > soil parent material > former land uses > age and sex of gardener > gardening practices > socio-professional group > type and proportion of productions > climate > age of the garden > size of the garden > education, information > cultural origin > functions of the garden > regulations). a typology of gardens including 7 selected factors and associated categories and a method for describing, sampling and characterizing a population of gardens representative (for a country) are proposed. based on the statistical analysis and data mining on regional databases, we have determined and proposed an optimum size for the collected population of garden soils. the analysis of a dataset of garden soils characteristics using data mining lead to regression tree models enabling us an easy understanding of the results on the “garden” system (e.g. predict total metal concentrations from agronomic parameters and land-use, percentage of coarse fraction and percentage of technogenic materials in garden soils). the discussion of the results of data mining highlights the main indicators of soil quality and the method for a survey of garden soils is proposed. these results and the resulting approach might be validated and used on a worldwide scale to collect garden soil samples with the objective of agronomic, environmental and sanitary studies adapted to this type of urban agriculture.}, note = {ACTI}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } In france and all over the world, there is no systematic data available on the quality (fertility and contamination) of garden soils. nevertheless, there is a growing need for a typology and for a method dedicated to national and international garden soil survey. this inventory is much needed in the context of environmental risk assessment, to predict the potential impact on human health of the direct contact with garden soils and of the consumption of vegetables from gardens. the state of the art on the international knowledge on garden soils, gardening practices and food production, shows that gardens remain poorly known and very complex ecological, economical and social systems. their global quality is the result of a wide number of factors including environment, history, specific characteristics of the gardens, gardeners and their practices, plant and/or animal productions and socio-economic context. the aim is then to better know the determinism of the agronomic, environmental and sanitary properties of gardens as a function of gardening practices and their impact on the quality of soils and plants. we propose a definition of “garden” and more generally of all the field “garden”. the system “garden” is represented by attributes (soil and plant characteristics) and factors with various impacts (e.g. environment > soil parent material > former land uses > age and sex of gardener > gardening practices > socio-professional group > type and proportion of productions > climate > age of the garden > size of the garden > education, information > cultural origin > functions of the garden > regulations). a typology of gardens including 7 selected factors and associated categories and a method for describing, sampling and characterizing a population of gardens representative (for a country) are proposed. based on the statistical analysis and data mining on regional databases, we have determined and proposed an optimum size for the collected population of garden soils. the analysis of a dataset of garden soils characteristics using data mining lead to regression tree models enabling us an easy understanding of the results on the “garden” system (e.g. predict total metal concentrations from agronomic parameters and land-use, percentage of coarse fraction and percentage of technogenic materials in garden soils). the discussion of the results of data mining highlights the main indicators of soil quality and the method for a survey of garden soils is proposed. these results and the resulting approach might be validated and used on a worldwide scale to collect garden soil samples with the objective of agronomic, environmental and sanitary studies adapted to this type of urban agriculture. |
Auteurs : Waterlot Christophe; Bidar Géraldine; Pruvot Christelle; Douay Francis Analysis of cadmium in water extracts from contaminated soils with high arsenic and iron concentration levels Journal of Environmental Science and Engineering, 5, pp. 271-2801 @article{Waterlot2011, title = {Analysis of cadmium in water extracts from contaminated soils with high arsenic and iron concentration levels}, author = {Christophe Waterlot and Géraldine Bidar and Christelle Pruvot and Francis Douay}, year = {2011}, date = {2011-01-01}, journal = {Journal of Environmental Science and Engineering}, volume = {5}, pages = {271-280}, abstract = {Cadmium (cd) concentrations in the water extracts often contaminated soils by arsenic (as) with various iron (fe) contents were measured using inductively coupled plasma atomic emission spectrometry (icp-aes) and electrothermal atomic absorption spectrometry (etaas). due to the potential spectral interferences of as and fe in the quantification of cd, two methods were used for the background compensation. the first method was based on the use of a deuterium lamp (bgcd2 mode). the second one required a cd hollow cathode lamp pulsated with a current for which the intensity varies in the course of time (bgcsr mode). the results showed that the choices of the analytical technique and the method used for the background compensation depend on the cd, as and fe concentrations in the solution and the concentration ratios as/cd or/and fe/cd. in comparison with the icp-aes and the etaas in combination with the bgcd2 mode, it was shown that the high-speed self-reversal method (hssr) was a more appropriate method to correct as and fe spectral interferences during the cd measurements. on the other hand, depending on the cd concentration, it was established that no significant fe interference occurred during the cd determination even if fe concentration was 50 mg/l, reflecting the efficiency of the hssr method to overcome the fe interferences in the determination of cd concentration by etaas without any matrix modifier in water extracts}, note = {ACL}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {article} } Cadmium (cd) concentrations in the water extracts often contaminated soils by arsenic (as) with various iron (fe) contents were measured using inductively coupled plasma atomic emission spectrometry (icp-aes) and electrothermal atomic absorption spectrometry (etaas). due to the potential spectral interferences of as and fe in the quantification of cd, two methods were used for the background compensation. the first method was based on the use of a deuterium lamp (bgcd2 mode). the second one required a cd hollow cathode lamp pulsated with a current for which the intensity varies in the course of time (bgcsr mode). the results showed that the choices of the analytical technique and the method used for the background compensation depend on the cd, as and fe concentrations in the solution and the concentration ratios as/cd or/and fe/cd. in comparison with the icp-aes and the etaas in combination with the bgcd2 mode, it was shown that the high-speed self-reversal method (hssr) was a more appropriate method to correct as and fe spectral interferences during the cd measurements. on the other hand, depending on the cd concentration, it was established that no significant fe interference occurred during the cd determination even if fe concentration was 50 mg/l, reflecting the efficiency of the hssr method to overcome the fe interferences in the determination of cd concentration by etaas without any matrix modifier in water extracts |
Auteurs : Waterlot Christophe; Pelfrêne Aurélie; Douay Francis Lead mobility and bioaccessibility in kitchen garden soils (northern France) after immobilization with a mixture of phosphates 11th International Conference on the Biogeochemistry of Trace Elements (ICOBTE). 3-7 July 2011, Florence (Italy) @conference{Waterlot2011d, title = {Lead mobility and bioaccessibility in kitchen garden soils (northern France) after immobilization with a mixture of phosphates}, author = {Christophe Waterlot and Aurélie Pelfrêne and Francis Douay}, year = {2011}, date = {2011-01-01}, booktitle = {11th International Conference on the Biogeochemistry of Trace Elements (ICOBTE). 3-7 July 2011, Florence (Italy)}, note = {ACTI}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } |
Auteurs : Waterlot Christophe; Pruvot Christelle; Ciesielski Henri; Douay Francis Effects of a phosphorus amendment and the pH of water used for watering on the mobility and phytoavailability of Cd, Pb and Zn in highly contaminated kitchen garden soils Ecological engineering, 37(7), pp. 1081-10931 @article{Waterlot2011b, title = {Effects of a phosphorus amendment and the pH of water used for watering on the mobility and phytoavailability of Cd, Pb and Zn in highly contaminated kitchen garden soils}, author = {Christophe Waterlot and Christelle Pruvot and Henri Ciesielski and Francis Douay}, year = {2011}, date = {2011-01-01}, journal = {Ecological engineering}, volume = {37}, number = {7}, pages = {1081-1093}, abstract = {Studies on two lead and zinc smelters in northern france (metaleurop nord and umicore) showed that the level of metallic contamination of kitchen garden soils is higher than the agricultural soils located in the same environment. this results most particularly from cropping practices and the addition of various products. due to the physical and chemical parameters of these soils, the behaviour and transfer of pollutants towards various plants (grass, trees, and vegetables) may be perceptibly different than what is observed on agricultural soils. for a better understanding of pollutant behaviour in kitchen garden topsoils, the cd, pb and zn was fractionated using the sm&t protocol and various extracting solutions (cacl2, acetic acid, and citric acid) to evaluate their mobility in two highly contaminated soils chosen in the area affected by the past atmospheric emissions of the two smelters. in addition, agricultural topsoil was sampled in a non-massively contaminated area and was therefore chosen as the control soil. the three soils were amended with a mixture of hydroxyapatite (ha) and diammonium phosphate (dap). at 6 months, extracting procedures were carried out to evaluate the effects of the amendment on the mobility of cd, pb and zn. this step was then supplemented by an evaluation of the impact of the amendment on the phytoavailability of pollutants, which was determined in plant uptake studies with ryegrass (lolium perenne l.) by considering only the pollutant concentrations in their shoots. two experiments were carried out. in the first one, unamended and amended soils and ryegrass were watered with distilled water (ph = 7). in the second one, osmosed water (ph = 5.5) was used to evaluate the effects of the acid water–phosphate amendment system on the mobility and phytoavailability of cd, pb and zn. six months after the start of the experiments, the selective extractions showed that the effectiveness of the amendment studied depended on the element, the soil and the water's ph. reductions of metal eluted from the contaminated soils were 1.5–37.9% for cd, and 9.1–80.9% for pb. application of p amendment to the combination of osmosed water was generally the most effective for immobilising cd and pb elution. in contrast, the mixture of ha and dap was ineffective for reducing zn elution. the plant-fresh biomass yield was significantly (p < 0.05) increased by the combination of p amendment and distilled water, whereas a reduction of biomass was recorded with the combined amendment and osmosed water. addition of p amendment generally reduced pb uptake in ryegrass shoots (1–47%), while both cd and zn were increased by 17.9–79% and 0.45–100%, respectively.}, note = {ACL}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {article} } Studies on two lead and zinc smelters in northern france (metaleurop nord and umicore) showed that the level of metallic contamination of kitchen garden soils is higher than the agricultural soils located in the same environment. this results most particularly from cropping practices and the addition of various products. due to the physical and chemical parameters of these soils, the behaviour and transfer of pollutants towards various plants (grass, trees, and vegetables) may be perceptibly different than what is observed on agricultural soils. for a better understanding of pollutant behaviour in kitchen garden topsoils, the cd, pb and zn was fractionated using the sm&t protocol and various extracting solutions (cacl2, acetic acid, and citric acid) to evaluate their mobility in two highly contaminated soils chosen in the area affected by the past atmospheric emissions of the two smelters. in addition, agricultural topsoil was sampled in a non-massively contaminated area and was therefore chosen as the control soil. the three soils were amended with a mixture of hydroxyapatite (ha) and diammonium phosphate (dap). at 6 months, extracting procedures were carried out to evaluate the effects of the amendment on the mobility of cd, pb and zn. this step was then supplemented by an evaluation of the impact of the amendment on the phytoavailability of pollutants, which was determined in plant uptake studies with ryegrass (lolium perenne l.) by considering only the pollutant concentrations in their shoots. two experiments were carried out. in the first one, unamended and amended soils and ryegrass were watered with distilled water (ph = 7). in the second one, osmosed water (ph = 5.5) was used to evaluate the effects of the acid water–phosphate amendment system on the mobility and phytoavailability of cd, pb and zn. six months after the start of the experiments, the selective extractions showed that the effectiveness of the amendment studied depended on the element, the soil and the water's ph. reductions of metal eluted from the contaminated soils were 1.5–37.9% for cd, and 9.1–80.9% for pb. application of p amendment to the combination of osmosed water was generally the most effective for immobilising cd and pb elution. in contrast, the mixture of ha and dap was ineffective for reducing zn elution. the plant-fresh biomass yield was significantly (p < 0.05) increased by the combination of p amendment and distilled water, whereas a reduction of biomass was recorded with the combined amendment and osmosed water. addition of p amendment generally reduced pb uptake in ryegrass shoots (1–47%), while both cd and zn were increased by 17.9–79% and 0.45–100%, respectively. |
Auteurs : Baize Denis; Douay Francis; Villanneau Estelle; Bourennane Hocine; Sterckeman Thibault; Ciesielski Henri; King Dominique Les éléments en traces dans les sols agricoles du Nord-Pas-de-Calais. I. Étude et cartographie des teneurs des horizons de surface Etude et Gestion des Sols, 17(3-4), pp. 213-2371 @article{Baize2010, title = {Les éléments en traces dans les sols agricoles du Nord-Pas-de-Calais. I. Étude et cartographie des teneurs des horizons de surface}, author = {Denis Baize and Francis Douay and Estelle Villanneau and Hocine Bourennane and Thibault Sterckeman and Henri Ciesielski and Dominique King}, year = {2010}, date = {2010-01-01}, journal = {Etude et Gestion des Sols}, volume = {17}, number = {3-4}, pages = {213-237}, abstract = {La base de données utilisée pour la présente étude rassemble des populations d’analyses de sols provenant de cinq sources correspondant chacune à une stratégie d’échantillonnage différente et à un objectif distinct. au total, 3 929 analyses réalisées entre 1990 et 2007 ont été collectées : elles provenaient presque exclusivement d’horizons de surface de sols agricoles. outre les concentrations en etm, les principales caractéristiques agro-pédologiques et les coordonnées géographiques, la base de données contient également des informations sur l’occupation du sol et sur la nature du matériau parental. dans le nord -pas-de-calais affleurent des roches sédimentaires variées, souvent recouvertes par des dépôts éoliens limoneux, mais tous ces matériaux montrent des teneurs naturelles faibles en etm. les différentes stratégies d’échantillonnage se sont assez bien complétées et ont permis une bonne couverture du territoire régional et des diverses causes de contaminations anthropiques : aussi bien les sources diffuses que celles très ponctuelles. ont été réalisés divers traitements tels que : statistiques régionales descriptives ; examen de la localisation des valeurs anomaliques ; cartographies mono-élémentaires par krigeage ; vision multi-élémentaire grâce à un indice numérique additif très simple. un fort contraste apparaît entre des secteurs fortement influencés par des contaminations voire des pollutions multimétalliques (zones riveraines de l’agglomération lilloise, environs immédiats de grands sites industriels) et les zones rurales qui ne montrent pas de contaminations nettes à l’exception du cadmium apporté par la fertilisation phosphatée. outre une vision spatiale des teneurs pour les 8 principaux éléments traces, les traitements ont permis d’avoir une bonne connaissance statistique des teneurs usuelles en stratifiant par matériaux parentaux et par petites régions naturelles. ces « teneurs agricoles habituelles » (tah) ainsi stratifiées peuvent être correctement estimées en considérant toutes les valeurs comprises entre le percentile 10 et le percentile 90. ces tah peuvent être utilisées comme valeurs de référence plus appropriées que de simples statistiques calculées à l’échelon régional ou départemental, tout particulièrement dans le cadre de l’interprétation de l’état des milieux ou pour proposer aux services santé-environnement des agences régionales de santé des valeurs seuils de sélection pour prendre en compte tel ou tel élément trace dans une évaluation des risques sanitaires}, note = {ACLN}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {article} } La base de données utilisée pour la présente étude rassemble des populations d’analyses de sols provenant de cinq sources correspondant chacune à une stratégie d’échantillonnage différente et à un objectif distinct. au total, 3 929 analyses réalisées entre 1990 et 2007 ont été collectées : elles provenaient presque exclusivement d’horizons de surface de sols agricoles. outre les concentrations en etm, les principales caractéristiques agro-pédologiques et les coordonnées géographiques, la base de données contient également des informations sur l’occupation du sol et sur la nature du matériau parental. dans le nord -pas-de-calais affleurent des roches sédimentaires variées, souvent recouvertes par des dépôts éoliens limoneux, mais tous ces matériaux montrent des teneurs naturelles faibles en etm. les différentes stratégies d’échantillonnage se sont assez bien complétées et ont permis une bonne couverture du territoire régional et des diverses causes de contaminations anthropiques : aussi bien les sources diffuses que celles très ponctuelles. ont été réalisés divers traitements tels que : statistiques régionales descriptives ; examen de la localisation des valeurs anomaliques ; cartographies mono-élémentaires par krigeage ; vision multi-élémentaire grâce à un indice numérique additif très simple. un fort contraste apparaît entre des secteurs fortement influencés par des contaminations voire des pollutions multimétalliques (zones riveraines de l’agglomération lilloise, environs immédiats de grands sites industriels) et les zones rurales qui ne montrent pas de contaminations nettes à l’exception du cadmium apporté par la fertilisation phosphatée. outre une vision spatiale des teneurs pour les 8 principaux éléments traces, les traitements ont permis d’avoir une bonne connaissance statistique des teneurs usuelles en stratifiant par matériaux parentaux et par petites régions naturelles. ces « teneurs agricoles habituelles » (tah) ainsi stratifiées peuvent être correctement estimées en considérant toutes les valeurs comprises entre le percentile 10 et le percentile 90. ces tah peuvent être utilisées comme valeurs de référence plus appropriées que de simples statistiques calculées à l’échelon régional ou départemental, tout particulièrement dans le cadre de l’interprétation de l’état des milieux ou pour proposer aux services santé-environnement des agences régionales de santé des valeurs seuils de sélection pour prendre en compte tel ou tel élément trace dans une évaluation des risques sanitaires |
Auteurs : Bernard Fabien; Brulle Franck; Douay Francis; Lemière Sébastien; Demuynck Sylvain; Vandenbulcke Franck Metallic trace element body burdens and gene expression analysis of biomarker candidates in Eisenia fetida, using an ''exposure/depuration'' experimental scheme with field soils Ecotoxicology and Environmental Safety, 73(5), pp. 1034-10451 @article{Bernard2010, title = {Metallic trace element body burdens and gene expression analysis of biomarker candidates in Eisenia fetida, using an ''exposure/depuration'' experimental scheme with field soils}, author = {Fabien Bernard and Franck Brulle and Francis Douay and Sébastien Lemière and Sylvain Demuynck and Franck Vandenbulcke}, year = {2010}, date = {2010-01-01}, journal = {Ecotoxicology and Environmental Safety}, volume = {73}, number = {5}, pages = {1034-1045}, abstract = {Smelting plant activities lead to the accumulation of metal trace elements (mtes) in soils. the presence of high concentrations of mtes can generate an environmental stress likely to affect macroinvertebrates living in close soil contact such as the annelida oligochaeta. eisenia fetida, an ecotoxicologically important test species, was successively exposed to two field soils: (1) a highly contaminated agricultural topsoil collected near the former smelter metaleurop nord (noyelles-godault, france) which contaminated surrounding soils by its atmospheric emissions [exposure phase], and then (2) a slightly contaminated topsoil from an urban garden located in the conurbation of lille (wambrechies) [depuration phase]. two analyses were performed during each phase. firstly, the gene expression levels of four biomarker candidates identified in previous studies were analyzed in e. fetida coelomocytes. these candidates are cd-metallothionein, phytochelatin synthase, coactosin-like protein and lysenin. secondly, the body burdens of the following elements cd, pb, zn, cu, fe, ca, and p were measured. moreover, both analyses were also performed in lumbricus rubellus, an annelid species collected from the two tested soil-originating sites. analysis of gene expression and mte body burdens in both species are discussed to: (1) evaluate expression biomarkers; (2) gain insight the detoxification processes and the long-term response to a metallic stress and (3) compare the responses observed in a test species (e. fetida) with the responses of a field species (l. rubellus). (c) 2010 elsevier inc. all rights reserved.}, note = {ACL}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {article} } Smelting plant activities lead to the accumulation of metal trace elements (mtes) in soils. the presence of high concentrations of mtes can generate an environmental stress likely to affect macroinvertebrates living in close soil contact such as the annelida oligochaeta. eisenia fetida, an ecotoxicologically important test species, was successively exposed to two field soils: (1) a highly contaminated agricultural topsoil collected near the former smelter metaleurop nord (noyelles-godault, france) which contaminated surrounding soils by its atmospheric emissions [exposure phase], and then (2) a slightly contaminated topsoil from an urban garden located in the conurbation of lille (wambrechies) [depuration phase]. two analyses were performed during each phase. firstly, the gene expression levels of four biomarker candidates identified in previous studies were analyzed in e. fetida coelomocytes. these candidates are cd-metallothionein, phytochelatin synthase, coactosin-like protein and lysenin. secondly, the body burdens of the following elements cd, pb, zn, cu, fe, ca, and p were measured. moreover, both analyses were also performed in lumbricus rubellus, an annelid species collected from the two tested soil-originating sites. analysis of gene expression and mte body burdens in both species are discussed to: (1) evaluate expression biomarkers; (2) gain insight the detoxification processes and the long-term response to a metallic stress and (3) compare the responses observed in a test species (e. fetida) with the responses of a field species (l. rubellus). (c) 2010 elsevier inc. all rights reserved. |
Auteurs : Bourennane Hocine; Douay Francis; Sterckeman Thibault; Villanneau Estelle; Ciesielski Henri; King Dominique; Baize Denis Mapping of anthropogenic trace elements inputs in agricultural topsoil from Northern France using enrichment factors Geoderma, 157(3-4), pp. 165-1741 @article{Bourennane2010, title = {Mapping of anthropogenic trace elements inputs in agricultural topsoil from Northern France using enrichment factors}, author = {Hocine Bourennane and Francis Douay and Thibault Sterckeman and Estelle Villanneau and Henri Ciesielski and Dominique King and Denis Baize}, year = {2010}, date = {2010-01-01}, journal = {Geoderma}, volume = {157}, number = {3-4}, pages = {165-174}, abstract = {Anthropogenic trace element contamination of soils in the densely populated and industrialized nord-pas de calais region (france) was quantified using enrichment factors (efs). total concentrations of al and 18 trace elements (as, bi, cd, co, cr, cu, hg, in, mn, mo, ni, pb, sb, se, sn, tl, v, and zn) were determined in 252 sampling sites both in topsoil and deep horizons of soils developed from sedimentary materials. thus, at each sampling site an enrichment factor was computed for each of the 18 trace elements as the ratio between total concentrations of the trace element in topsoil and deep horizons in the same soil pit, normalized by concentrations of al measured in the same horizons and the same pit. to estimate ef values at unsampled locations and then to assess the spatial magnitude of anthropogenic trace element contamination over the whole study area (12400 km(2)), variography analysis was used to quantify the spatial structure of each ef. results have shown that values of 8 efs are spatially correlated. these efs were thus mapped using the sequential gaussian simulation technique. one hundred horizon samples from 50 supplementary sites were analysed for the structured trace elements and al contents and used for the mapping validation. results of the validation based on the accuracy plots and goodness statistics (g and d) allowed us to conclude that ef maps exhibited high certainty in regard to the validation data set. in addition, results have shown that e-type estimates were accurate when they are used to estimate the measured ef value of the validation data set. accordingly, these maps were used to discuss the origin of patches of surface horizon enrichment displaying an exogenous input of the trace elements investigated. the whole study area under discussion is strongly enriched with cadmium, originating from both industrial and agricultural activities. surface horizons also exhibit locally high levels of pb and zn enrichment, mainly around well-known former or current industrial sites. however. pb and zn enriched surface horizons are far less widespread than those enriched in cd. higher cu, bi and sn ef values were observed in the northern part of the region studied than in the southern part. the dissimilarity underlined a rather diffuse contamination from the urbanized zone in the north to the rural zone in the south of the region. the enrichment of surface horizons by in and tl seems insignificant. some unstructured efs were attributes to point-source enrichments. (c) 2010 elsevier b.v. all rights reserved.}, note = {ACL}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {article} } Anthropogenic trace element contamination of soils in the densely populated and industrialized nord-pas de calais region (france) was quantified using enrichment factors (efs). total concentrations of al and 18 trace elements (as, bi, cd, co, cr, cu, hg, in, mn, mo, ni, pb, sb, se, sn, tl, v, and zn) were determined in 252 sampling sites both in topsoil and deep horizons of soils developed from sedimentary materials. thus, at each sampling site an enrichment factor was computed for each of the 18 trace elements as the ratio between total concentrations of the trace element in topsoil and deep horizons in the same soil pit, normalized by concentrations of al measured in the same horizons and the same pit. to estimate ef values at unsampled locations and then to assess the spatial magnitude of anthropogenic trace element contamination over the whole study area (12400 km(2)), variography analysis was used to quantify the spatial structure of each ef. results have shown that values of 8 efs are spatially correlated. these efs were thus mapped using the sequential gaussian simulation technique. one hundred horizon samples from 50 supplementary sites were analysed for the structured trace elements and al contents and used for the mapping validation. results of the validation based on the accuracy plots and goodness statistics (g and d) allowed us to conclude that ef maps exhibited high certainty in regard to the validation data set. in addition, results have shown that e-type estimates were accurate when they are used to estimate the measured ef value of the validation data set. accordingly, these maps were used to discuss the origin of patches of surface horizon enrichment displaying an exogenous input of the trace elements investigated. the whole study area under discussion is strongly enriched with cadmium, originating from both industrial and agricultural activities. surface horizons also exhibit locally high levels of pb and zn enrichment, mainly around well-known former or current industrial sites. however. pb and zn enriched surface horizons are far less widespread than those enriched in cd. higher cu, bi and sn ef values were observed in the northern part of the region studied than in the southern part. the dissimilarity underlined a rather diffuse contamination from the urbanized zone in the north to the rural zone in the south of the region. the enrichment of surface horizons by in and tl seems insignificant. some unstructured efs were attributes to point-source enrichments. (c) 2010 elsevier b.v. all rights reserved. |
Auteurs : Brulle Franck; Cocquerelle Claude; Lemière Sébastien; Douay Francis; Leprêtre Alain; Vandenbulcke Franck Identification and analysis of the expression profiles of new biomarker candidates in Eisenia fetida: A transcriptomic approach SETAC Europe 20th Annual Meeting. 23-27 May 2010, Seville (Espagne) @conference{Brulle2010a, title = {Identification and analysis of the expression profiles of new biomarker candidates in Eisenia fetida: A transcriptomic approach}, author = {Franck Brulle and Claude Cocquerelle and Sébastien Lemière and Francis Douay and Alain Leprêtre and Franck Vandenbulcke}, year = {2010}, date = {2010-01-01}, booktitle = {SETAC Europe 20th Annual Meeting. 23-27 May 2010, Seville (Espagne)}, note = {COM}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } |
Auteurs : Fritsch Clémentine; Giraudoux Patrick; Coeurdassier Michaël; Douay Francis; Raoul Francis; Pruvot Christelle; Waterlot Christophe; De-Vaufleury Annette; Scheifler Renaud Spatial distribution of metals in smelter-impacted soils of woody habitats: Influence of landscape and soil properties, and risk for wildlife Chemosphere, 81, pp. 141-1551 @article{Fritsch2010, title = {Spatial distribution of metals in smelter-impacted soils of woody habitats: Influence of landscape and soil properties, and risk for wildlife}, author = {Clémentine Fritsch and Patrick Giraudoux and Michaël Coeurdassier and Francis Douay and Francis Raoul and Christelle Pruvot and Christophe Waterlot and Annette De-Vaufleury and Renaud Scheifler}, year = {2010}, date = {2010-01-01}, journal = {Chemosphere}, volume = {81}, pages = {141-155}, abstract = {The spatial distribution of total and cacl2-extracted cd, pb and zn concentrations in smelter-impacted soils was investigated over a polluted site (40 km2) in northern france. the study was conducted on 262 soils sampled in woody habitats. total and extracted concentrations of trace metals (tms) rose up to 2402 and 59.5 mg kg1 for cd, 41 960 and 13.7 mg kg1 for pb, 38 760 and 143.0 mg kg1 for zn, respectively. the spatial dependence of both total and extracted concentrations showed a high spatial auto-correlation and ordinary kriging was used to predict soil concentrations. investigating which variables influenced metal concentrations and their spatial distribution, we found that total concentrations mostly depended on the distance from the smelter, the wind and the organic carbon, while extracted concentrations were mainly modulated by the ph. conditionally to those variables, other soil properties and landscape characteristics influenced both total and extracted concentrations. we conclude that total and extracted tm concentrations are governed by different processes which act at various spatial ranges: total concentrations are mainly related to input and retention of metals (large scale) whereas extracted concentrations were mainly explained by factors controlling metal solubility in soils (local scale). spatial distributions of total and extracted tms differed over the area, which should be considered for risk assessment. maps of risk based on the us epa’s eco-ssls (for plants, invertebrates and vertebrates) were realized, showing that wildlife may be at risk but that the relevance of eco-ssl values is questionable.}, note = {ACL}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {article} } The spatial distribution of total and cacl2-extracted cd, pb and zn concentrations in smelter-impacted soils was investigated over a polluted site (40 km2) in northern france. the study was conducted on 262 soils sampled in woody habitats. total and extracted concentrations of trace metals (tms) rose up to 2402 and 59.5 mg kg1 for cd, 41 960 and 13.7 mg kg1 for pb, 38 760 and 143.0 mg kg1 for zn, respectively. the spatial dependence of both total and extracted concentrations showed a high spatial auto-correlation and ordinary kriging was used to predict soil concentrations. investigating which variables influenced metal concentrations and their spatial distribution, we found that total concentrations mostly depended on the distance from the smelter, the wind and the organic carbon, while extracted concentrations were mainly modulated by the ph. conditionally to those variables, other soil properties and landscape characteristics influenced both total and extracted concentrations. we conclude that total and extracted tm concentrations are governed by different processes which act at various spatial ranges: total concentrations are mainly related to input and retention of metals (large scale) whereas extracted concentrations were mainly explained by factors controlling metal solubility in soils (local scale). spatial distributions of total and extracted tms differed over the area, which should be considered for risk assessment. maps of risk based on the us epa’s eco-ssls (for plants, invertebrates and vertebrates) were realized, showing that wildlife may be at risk but that the relevance of eco-ssl values is questionable. |
Auteurs : Hedde Mickaël; Bispo Antonio; Cluzeau Daniel; Cortet Jérôme; Douay Francis; Galsomies Laurence; Grand Cécile; Houot Sabine; Leyval Corinne; Pauget Benjamin; Ruiz-Camacho Nuria; Ulrich Erwin; Vandenbulcke Franck; de-Vaufleury Annette; Villenave Cécile; Pérès Guénola Recherche d’outils de surveillance, de caractérisation et d’évaluation des risques des sols Programme ADEME - Bioindicateur II : bio-indicateurs basés sur la faune du sol. Colloque de la Société Française d’Ecotoxicologie Fondamentale et Appliquée @conference{Hedde2010, title = {Recherche d’outils de surveillance, de caractérisation et d’évaluation des risques des sols}, author = {Mickaël Hedde and Antonio Bispo and Daniel Cluzeau and Jérôme Cortet and Francis Douay and Laurence Galsomies and Cécile Grand and Sabine Houot and Corinne Leyval and Benjamin Pauget and Nuria Ruiz-Camacho and Erwin Ulrich and Franck Vandenbulcke and Annette de-Vaufleury and Cécile Villenave and Guénola Pérès}, year = {2010}, date = {2010-01-01}, booktitle = {Programme ADEME - Bioindicateur II : bio-indicateurs basés sur la faune du sol. Colloque de la Société Française d’Ecotoxicologie Fondamentale et Appliquée}, note = {ACTN}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } |
Auteurs : Lemière Sébastien; Godet Jean-Philippe; Tanguy Marion; Grumiaux Fabien; Leroux Régine; Vandenbulcke Franck; Demuynck Sylvain; Pernin Céline; Pruvot Christelle; Delplace Patrick; Douay Francis; Leprêtre Alain Indoor microcosm studies for ecotoxicological evaluation of metal contaminated soil remediation agents SETAC Europe 20th Annual Meeting. 23-27 May 2010, Seville (Espagne) @conference{Lemiere2010a, title = {Indoor microcosm studies for ecotoxicological evaluation of metal contaminated soil remediation agents}, author = {Sébastien Lemière and Jean-Philippe Godet and Marion Tanguy and Fabien Grumiaux and Régine Leroux and Franck Vandenbulcke and Sylvain Demuynck and Céline Pernin and Christelle Pruvot and Patrick Delplace and Francis Douay and Alain Leprêtre}, year = {2010}, date = {2010-01-01}, booktitle = {SETAC Europe 20th Annual Meeting. 23-27 May 2010, Seville (Espagne)}, note = {ACTI}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } |
Auteurs : Pohu-Lopareva Alena; Roussel Hélène; Waterlot Christophe; Garçon Guillaume; Bidar Géraldine; Pruvot Christelle; Baize Denis; Shirali A; Douay Francis Asymétrie fluctuante des feuilles d’Alnus glutinosa: Indicateur de stress dans le cadre d’une phytostabilisation aidée sur des sols fortement pollués par des éléments traces métalliques Bulletin Bodenkundliche Gesellschaft der Schweiz, 30, pp. 51-561 @article{Pohu-Lopareva2010, title = {Asymétrie fluctuante des feuilles d’Alnus glutinosa: Indicateur de stress dans le cadre d’une phytostabilisation aidée sur des sols fortement pollués par des éléments traces métalliques}, author = {Alena Pohu-Lopareva and Hélène Roussel and Christophe Waterlot and Guillaume Garçon and Géraldine Bidar and Christelle Pruvot and Denis Baize and A Shirali and Francis Douay}, year = {2010}, date = {2010-01-01}, journal = {Bulletin Bodenkundliche Gesellschaft der Schweiz}, volume = {30}, pages = {51-56}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {article} } |
Auteurs : Roussel Hélène; Waterlot Christophe; Pelfrêne Aurélie; Pruvot Christelle; Mazzuca Muriel; Douay Francis Cd, Pb and Zn oral bioaccessibility of urban soils contaminated in the past by atmospheric emissions from two lead and zinc smelters Archives of Environmental Contamination and Toxicology, 58(4), pp. 945-9541 @article{Roussel2010, title = {Cd, Pb and Zn oral bioaccessibility of urban soils contaminated in the past by atmospheric emissions from two lead and zinc smelters}, author = {Hélène Roussel and Christophe Waterlot and Aurélie Pelfrêne and Christelle Pruvot and Muriel Mazzuca and Francis Douay}, year = {2010}, date = {2010-01-01}, journal = {Archives of Environmental Contamination and Toxicology}, volume = {58}, number = {4}, pages = {945-954}, abstract = {Ingestion of dust or soil particles could pose a potential health risk due to long-term metal trace element (mte) exposure. twenty-seven urban topsoil samples (kitchen garden and lawn) were collected and analyzed for cd, pb and zn using the unified bioaccessibility research group of europe (barge) method (ubm) test to estimate the human bioaccessibility of these elements. the quantities of cd, pb and zn extracted from soils indicated, on average, 68, 62 and 47% bioaccessibility, respectively, in the gastric phase and 31, 32 and 23% bioaccessibility, respectively, in the gastro-intestinal phase. significant positive correlations were observed between concentrations extracted with ubm and total mte contents. stepwise multiple regression analysis showed that human bioaccessibility was also affected by some physico-chemical soil parameters (i.e. total nitrogen, carbonates, clay contents and ph). the unified test presents some valuable data for risk assessment. indeed, the incorporation of oral bioaccessible concentrations into risk estimations could give more realistic information for health risk assessment.}, note = {ACL}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {article} } Ingestion of dust or soil particles could pose a potential health risk due to long-term metal trace element (mte) exposure. twenty-seven urban topsoil samples (kitchen garden and lawn) were collected and analyzed for cd, pb and zn using the unified bioaccessibility research group of europe (barge) method (ubm) test to estimate the human bioaccessibility of these elements. the quantities of cd, pb and zn extracted from soils indicated, on average, 68, 62 and 47% bioaccessibility, respectively, in the gastric phase and 31, 32 and 23% bioaccessibility, respectively, in the gastro-intestinal phase. significant positive correlations were observed between concentrations extracted with ubm and total mte contents. stepwise multiple regression analysis showed that human bioaccessibility was also affected by some physico-chemical soil parameters (i.e. total nitrogen, carbonates, clay contents and ph). the unified test presents some valuable data for risk assessment. indeed, the incorporation of oral bioaccessible concentrations into risk estimations could give more realistic information for health risk assessment. |
Auteurs : Waterlot Christophe; Douay Francis Evaluation des effets d’une lampe à cathode creuse pulsée à courant variable sur les interférences spectrales de l’arsenic dans le dosage du cadmium par spectrophotométrie d’absorption atomique Revue Canadienne de Génie Civil, 37, pp. 346-3531 @article{Waterlot2010a, title = {Evaluation des effets d’une lampe à cathode creuse pulsée à courant variable sur les interférences spectrales de l’arsenic dans le dosage du cadmium par spectrophotométrie d’absorption atomique}, author = {Christophe Waterlot and Francis Douay}, year = {2010}, date = {2010-01-01}, journal = {Revue Canadienne de Génie Civil}, volume = {37}, pages = {346-353}, abstract = {Les teneurs en cadmium de solutions aqueuses contaminees a des teneurs croissantes en arsenic ont ete mesurees a l’aide d’un spectrophotome`tre d’absorption atomique equipe d’une flamme et d’un four electrothermique. pour chacune des techniques d’atomisation, a ete evaluee une me´thode de correction du bruit de fond base´e sur le renversement de spectre et connue sous le nom de « high-speed self-reversal method », au regard d’une autre methode plus couramment utilisee ne ne´cessitant qu’une lampe au deute´rium et une lampe au cadmium. en comparaison avec cette me´thode, les re- sultats ont montre que le mode de correction base´ sur le renversement spectral via l’utilisation d’une lampe au cadmium a` cathode creuse pulse´e a` courant variable etait une methode plus approprie´e pour corriger les phe´nome`nes d’interfe´rences causes par l’arsenic (as) lors du dosage du cadmium (cd). il a ete etabli que cette methode permettait de s’affranchir des interferences de l’arsenic pour des ratios [as]/[cd] infe´rieurs a` 50 000.}, note = {ACLN}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {article} } Les teneurs en cadmium de solutions aqueuses contaminees a des teneurs croissantes en arsenic ont ete mesurees a l’aide d’un spectrophotome`tre d’absorption atomique equipe d’une flamme et d’un four electrothermique. pour chacune des techniques d’atomisation, a ete evaluee une me´thode de correction du bruit de fond base´e sur le renversement de spectre et connue sous le nom de « high-speed self-reversal method », au regard d’une autre methode plus couramment utilisee ne ne´cessitant qu’une lampe au deute´rium et une lampe au cadmium. en comparaison avec cette me´thode, les re- sultats ont montre que le mode de correction base´ sur le renversement spectral via l’utilisation d’une lampe au cadmium a` cathode creuse pulse´e a` courant variable etait une methode plus approprie´e pour corriger les phe´nome`nes d’interfe´rences causes par l’arsenic (as) lors du dosage du cadmium (cd). il a ete etabli que cette methode permettait de s’affranchir des interferences de l’arsenic pour des ratios [as]/[cd] infe´rieurs a` 50 000. |
Auteurs : Bernard Fabien; Brulle Franck; Douay Francis; Lemière Sébastien; Demuynck Sylvain; Leprêtre Alain; Vandenbulcke Franck Metallic Trace Elements body burden and gene expression analysis of biomarker candidates in Eisenia fetida, during an “intox-detox” protocol performed with field soils SETAC Europe 19th Annual Meeting, 31 May-4 June 2009, Göteborg (Suède) @conference{Bernard2009, title = {Metallic Trace Elements body burden and gene expression analysis of biomarker candidates in Eisenia fetida, during an “intox-detox” protocol performed with field soils}, author = {Fabien Bernard and Franck Brulle and Francis Douay and Sébastien Lemière and Sylvain Demuynck and Alain Leprêtre and Franck Vandenbulcke}, year = {2009}, date = {2009-01-01}, booktitle = {SETAC Europe 19th Annual Meeting, 31 May-4 June 2009, Göteborg (Suède)}, note = {ACTI}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } |
Auteurs : Bidar Géraldine; Garçon Guillaume; Pruvot Christelle; Verdin Anthony; Shirali Pirouz; Douay Francis Seasonal and annual variations of the metal uptake and toxicity in Trifolium repens and Lolium perenne growing in a heavy metal contaminated field Environmental Science and Pollution Research, 16(1), pp. 42-531 @article{Bidar2009, title = {Seasonal and annual variations of the metal uptake and toxicity in Trifolium repens and Lolium perenne growing in a heavy metal contaminated field}, author = {Géraldine Bidar and Guillaume Garçon and Christelle Pruvot and Anthony Verdin and Pirouz Shirali and Francis Douay}, year = {2009}, date = {2009-01-01}, journal = {Environmental Science and Pollution Research}, volume = {16}, number = {1}, pages = {42-53}, abstract = {Background, aim, and scope the reclamation of nonferrous metal-polluted soil by phytoremediation requires an overall and permanent plant cover. to select the most suitable plant species, it is necessary to study metal effects on plants over the time, thereby checking that metals remain stored in root systems and not transferred to aerial parts. in this purpose, the seasonal and annual variations of metal bioaccumulation, transfer, and phytotoxicity in trifolium repens and lolium perenne grown in a cd-, pb-, and zn-contaminated soil were also studied. materials and methods the experimental site was located near a closed smelter. in spring 2004, two areas were sown with t. repens and l. perenne, respectively. thereafter, the samplings of plant roots and shoots and surrounding soils were realized in autumn 2004 and spring and autumn 2005. the soil agronomic characteristics, the cd, pb, and zn concentrations in the surrounded soils and plant organs, as well as the oxidative alterations (superoxide dismutase [sod], malondialdehyde [mda], and 8-hydroxy-2′-deoxyguanosine [8-ohdg]) in plant organs were carried out. results whatever the sampling period, metal concentrations in soils and plants were higher than background values. contrary to the soils, the fluctuations of metal concentrations were observed in plant organs over the time. bioaccumulation and transfer factors confirmed that metals were preferentially accumulated in the roots as follows: cd>zn>pb, and their transfer to shoots was limited. foliar metal deposition was also observed. the results showed that there were seasonal and annual variations of metal accumulation in the two studied plant species. these variations differed according to the organs and followed nearly the same pattern for the two species. oxidative alterations were observed in plant organs with regard to sod antioxidant activities, mda, and 8-ohdg concentrations. these alterations vary according to the temporal variations of metal concentrations. discussion metal concentrations in surrounded soils and plant organs showed the effective contamination by industrial dust emissions. metals absorbed by plants were mainly stored in the roots. with regard to this storage, the plants seemed to limit the metal transfer to their aerial parts over the time, thereby indicating their availability for metal phytostabilization. aerial deposition was another source of plant exposure to nonferrous metals. despite the occurrence ofmetal-induced oxidative alterations in plant organs, both plant species seemed to tolerate a high metal concentration in soils. conclusions taken together, these results indicated that t. repens and l. perenne were able to form a plant cover on highly cd-, pb-, and zn-polluted soils, to limit the metal transfer to their aerial parts and were relatively metal-tolerant. all these characteristics made them suitable for phytostabilization on metal-contaminated soils. these findings also highlighted the necessity to take into account seasonal and annual variations for a future phytomanagement. recommendations and perspectives in this work, the behavior of plant species grown in metal-polluted soil has been studied during 2 years. obviously, this time is too short to ensure that metals remain accumulated in the root system and few are transferred in aerial parts over the time. it is why regular monitoring should be achieved during more than a decade after the settlement of the plant cover. this work will be completed by the study of the t. repens and l. perenne effects on mobility of metals in order to evaluate the quantities of pollutants which could be absorbed by the biota and transferred to groundwater. bioaccessibility tests could be also realized on polluted soils in order to evaluate the phytostabilization impacts on the exposition risks for humans. keywords bioaccumulation factors . l. perenne . nonferrous metal-contaminated soil . oxidative stress . phytostabilization . seasonal and annual variations . t. repens . transfer factors}, note = {ACL}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {article} } Background, aim, and scope the reclamation of nonferrous metal-polluted soil by phytoremediation requires an overall and permanent plant cover. to select the most suitable plant species, it is necessary to study metal effects on plants over the time, thereby checking that metals remain stored in root systems and not transferred to aerial parts. in this purpose, the seasonal and annual variations of metal bioaccumulation, transfer, and phytotoxicity in trifolium repens and lolium perenne grown in a cd-, pb-, and zn-contaminated soil were also studied. materials and methods the experimental site was located near a closed smelter. in spring 2004, two areas were sown with t. repens and l. perenne, respectively. thereafter, the samplings of plant roots and shoots and surrounding soils were realized in autumn 2004 and spring and autumn 2005. the soil agronomic characteristics, the cd, pb, and zn concentrations in the surrounded soils and plant organs, as well as the oxidative alterations (superoxide dismutase [sod], malondialdehyde [mda], and 8-hydroxy-2′-deoxyguanosine [8-ohdg]) in plant organs were carried out. results whatever the sampling period, metal concentrations in soils and plants were higher than background values. contrary to the soils, the fluctuations of metal concentrations were observed in plant organs over the time. bioaccumulation and transfer factors confirmed that metals were preferentially accumulated in the roots as follows: cd>zn>pb, and their transfer to shoots was limited. foliar metal deposition was also observed. the results showed that there were seasonal and annual variations of metal accumulation in the two studied plant species. these variations differed according to the organs and followed nearly the same pattern for the two species. oxidative alterations were observed in plant organs with regard to sod antioxidant activities, mda, and 8-ohdg concentrations. these alterations vary according to the temporal variations of metal concentrations. discussion metal concentrations in surrounded soils and plant organs showed the effective contamination by industrial dust emissions. metals absorbed by plants were mainly stored in the roots. with regard to this storage, the plants seemed to limit the metal transfer to their aerial parts over the time, thereby indicating their availability for metal phytostabilization. aerial deposition was another source of plant exposure to nonferrous metals. despite the occurrence ofmetal-induced oxidative alterations in plant organs, both plant species seemed to tolerate a high metal concentration in soils. conclusions taken together, these results indicated that t. repens and l. perenne were able to form a plant cover on highly cd-, pb-, and zn-polluted soils, to limit the metal transfer to their aerial parts and were relatively metal-tolerant. all these characteristics made them suitable for phytostabilization on metal-contaminated soils. these findings also highlighted the necessity to take into account seasonal and annual variations for a future phytomanagement. recommendations and perspectives in this work, the behavior of plant species grown in metal-polluted soil has been studied during 2 years. obviously, this time is too short to ensure that metals remain accumulated in the root system and few are transferred in aerial parts over the time. it is why regular monitoring should be achieved during more than a decade after the settlement of the plant cover. this work will be completed by the study of the t. repens and l. perenne effects on mobility of metals in order to evaluate the quantities of pollutants which could be absorbed by the biota and transferred to groundwater. bioaccessibility tests could be also realized on polluted soils in order to evaluate the phytostabilization impacts on the exposition risks for humans. keywords bioaccumulation factors . l. perenne . nonferrous metal-contaminated soil . oxidative stress . phytostabilization . seasonal and annual variations . t. repens . transfer factors |
Auteurs : Brulle Franck; Cocquerelle Claude; Douay Francis; Lemière Sébastien; Leprêtre Alain; Vandenbulcke Franck Développement de biomarqueurs d’exposition aux métaux chez un organisme test: l’Annélide Oligochète Eisenia fetida 2ème Rencontres Nationales de la Recherche sur les Sites & Sols Pollués, ADEME. 20-21 octobre 2009, Paris @conference{Brulle2009a, title = {Développement de biomarqueurs d’exposition aux métaux chez un organisme test: l’Annélide Oligochète Eisenia fetida}, author = {Franck Brulle and Claude Cocquerelle and Francis Douay and Sébastien Lemière and Alain Leprêtre and Franck Vandenbulcke}, year = {2009}, date = {2009-01-01}, booktitle = {2ème Rencontres Nationales de la Recherche sur les Sites & Sols Pollués, ADEME. 20-21 octobre 2009, Paris}, abstract = {Couches supérieures des sols, où les concentrations peuvent être largement supérieures aux teneurs habituelles régionales. les annélides oligochètes sont présents dans ces sols pollués bien qu’ils présentent une sensibilité exacerbée aux métaux. très peu de choses étant connues quant à l’identification et la mise en place des mécanismes de réponse à ces métaux au niveau moléculaire, le travail de caractérisation des candidats biomarqueurs de pollution a été réalisé à partir des cellules immunitaires du ver eisenia fetida, une espèce modèle recommandée en écotoxicologie. deux approches ont été mises en place. une première approche, qualifiée de ciblée et basée sur la conservation phylogénique observée entre espèces, a permis l’identification de plusieurs candidats. une deuxième approche, dite globale et consistant en la construction de banques soustractives a permis d’identifier des gènes dont l’expression est affectée lors d’une exposition à un mélange complexe de métaux. enfin, les candidats biomarqueurs de pollution métallique mis en lumière par les deux approches ont été mesurés sur des animaux exposés à des sols pollués de longue date par des activités métallurgiques. ce travail a été réalisé dans le cadre d’une thèse de doctorat (f. brulle) financée par l’ademe et la région nord-pas-de-calais. 1- premièrement, nous avons cloné et analysé le niveau d’expression de 14 candidats biomarqueurs chez le modèle de laboratoire eisenia fetida exposé in vivo à des sols standards contaminés par le cadmium. les 14 candidats ont été choisis parmi des gènes codant des protéines fortement conservées pour lesquelles des variations d’expression génique ont été rapportées dans la littérature, lors d’exposition de macroinvertébrés à des etms. le niveau d’expression des gènes a été mesuré dans les coelomocytes (cellules immunitaires) puisque la majorité des effecteurs sélectionnés sont connus pour leur implication dans des mécanismes de défense [1].}, note = {ACTN}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } Couches supérieures des sols, où les concentrations peuvent être largement supérieures aux teneurs habituelles régionales. les annélides oligochètes sont présents dans ces sols pollués bien qu’ils présentent une sensibilité exacerbée aux métaux. très peu de choses étant connues quant à l’identification et la mise en place des mécanismes de réponse à ces métaux au niveau moléculaire, le travail de caractérisation des candidats biomarqueurs de pollution a été réalisé à partir des cellules immunitaires du ver eisenia fetida, une espèce modèle recommandée en écotoxicologie. deux approches ont été mises en place. une première approche, qualifiée de ciblée et basée sur la conservation phylogénique observée entre espèces, a permis l’identification de plusieurs candidats. une deuxième approche, dite globale et consistant en la construction de banques soustractives a permis d’identifier des gènes dont l’expression est affectée lors d’une exposition à un mélange complexe de métaux. enfin, les candidats biomarqueurs de pollution métallique mis en lumière par les deux approches ont été mesurés sur des animaux exposés à des sols pollués de longue date par des activités métallurgiques. ce travail a été réalisé dans le cadre d’une thèse de doctorat (f. brulle) financée par l’ademe et la région nord-pas-de-calais. 1- premièrement, nous avons cloné et analysé le niveau d’expression de 14 candidats biomarqueurs chez le modèle de laboratoire eisenia fetida exposé in vivo à des sols standards contaminés par le cadmium. les 14 candidats ont été choisis parmi des gènes codant des protéines fortement conservées pour lesquelles des variations d’expression génique ont été rapportées dans la littérature, lors d’exposition de macroinvertébrés à des etms. le niveau d’expression des gènes a été mesuré dans les coelomocytes (cellules immunitaires) puisque la majorité des effecteurs sélectionnés sont connus pour leur implication dans des mécanismes de défense [1]. |
Auteurs : Brulle Franck; Cocquerelle Claude; Douay Francis; Lemière Sébastien; Leprêtre Alain; Vandenbulcke Franck Développement de biomarqueurs d'exposition aux métaux chez un organisme test: l'Annélide Oligochète Eisenia fetida 15ème Colloque National de la Recherche en IUT, CNRIUT 2009. 8-10 juin 2009, Villeneuve d’Ascq @conference{Brulle2009b, title = {Développement de biomarqueurs d'exposition aux métaux chez un organisme test: l'Annélide Oligochète Eisenia fetida}, author = {Franck Brulle and Claude Cocquerelle and Francis Douay and Sébastien Lemière and Alain Leprêtre and Franck Vandenbulcke}, year = {2009}, date = {2009-01-01}, booktitle = {15ème Colloque National de la Recherche en IUT, CNRIUT 2009. 8-10 juin 2009, Villeneuve d’Ascq}, note = {ACTN}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } |
Auteurs : Brulle Franck; Douay Francis; Leprêtre Alain; Vandenbulcke Franck Gene expression analysis of 4 biomarker candidates in Eisenia fetida exposed to an environmental cadmium gradient: a microcosm study SETAC Europe 19th Annual Meeting, 31 May-4 June 2009, Göteborg (Suède) @conference{Brulle2009c, title = {Gene expression analysis of 4 biomarker candidates in Eisenia fetida exposed to an environmental cadmium gradient: a microcosm study}, author = {Franck Brulle and Francis Douay and Alain Leprêtre and Franck Vandenbulcke}, year = {2009}, date = {2009-01-01}, booktitle = {SETAC Europe 19th Annual Meeting, 31 May-4 June 2009, Göteborg (Suède)}, note = {ACTI}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } |
Auteurs : Douay Francis; Pruvot Christelle; Waterlot Christophe; Fritsch Clémentine; Fourrier Hervé; Loriette Alexandre; Bidar Géraldine; Grand Cécile; De-Vaufleury Annette; Scheifler Renaud Contamination of woody habitat soils around a former lead smelter in the North of France Science of the Total Environment, 407(21), pp. 5564-55771 @article{Douay2009, title = {Contamination of woody habitat soils around a former lead smelter in the North of France}, author = {Francis Douay and Christelle Pruvot and Christophe Waterlot and Clémentine Fritsch and Hervé Fourrier and Alexandre Loriette and Géraldine Bidar and Cécile Grand and Annette De-Vaufleury and Renaud Scheifler}, year = {2009}, date = {2009-01-01}, journal = {Science of the Total Environment}, volume = {407}, number = {21}, pages = {5564-5577}, abstract = {The Contamination of the Topsoil of 262 Woody Habitats around a Former Lead Smelter in the North of France Was Assessed. in This Urbanized and Industrialized Area, These Kinds of Habitats Comprise of Hedges, Groves, Small Woods, Anthropogenic Creations and One Large Forest. except for the Latter, Which Is 3 Km Away, These Woody Habitat Soils Often Present a High Anthropization Degree (a Significant Amount of Pebbles and Stones Related to Human Activities) with a High Metal Contamination. in the Studied Woody Habitat Topsoils, Cd, Pb and Zn Concentrations Largely Exceeded Those of Agricultural Topsoils Located in the Same Environmental Context. Therefore, Atmospheric Emissions from the Smelter Are Not the Only Cause of the High Contamination of the Woody Habitat Soils. This Last One Is Related to the Nature and the Contamination Level of Deposit in Relation with Human Activities (rubbles, Slag, Soils, Etc). with Regard to the Results Obtained with Chemical Extractions, the Mobility of Cd, Pb and Zn in These Soils Is Also Greater Than in Agricultural Soils. in the Forest, Pollutant Solubility Is Increased by Soil Acidic Ph. the Variability of the Physico-chemical Parameters and the High Metal Contamination of the Topsoils Are the Main Characteristics of the Woody Habitats Located around the Former Smelter. Although Never Taken into Account during Risk Assessment, the Disturbance of These Environmental Components Could Have Important Biogeochemical Impacts (nutrients and Metal Cycles). Moreover, Any Modification of the Soils' Use Could Potentially Cause Mobilization and Transfer of the Pollutants to the Biosphere. Six Years After the Closure of the Smelter, and As Social and Economic Pressures Considerably Increase in This Area, the Study of These Peculiar Ecosystems Is Necessary to Understand and Predict the Bioavailability, Transfer, Bioaccumulation and Effects of Pollutants in Food Chains.}, note = {ACL}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {article} } The Contamination of the Topsoil of 262 Woody Habitats around a Former Lead Smelter in the North of France Was Assessed. in This Urbanized and Industrialized Area, These Kinds of Habitats Comprise of Hedges, Groves, Small Woods, Anthropogenic Creations and One Large Forest. except for the Latter, Which Is 3 Km Away, These Woody Habitat Soils Often Present a High Anthropization Degree (a Significant Amount of Pebbles and Stones Related to Human Activities) with a High Metal Contamination. in the Studied Woody Habitat Topsoils, Cd, Pb and Zn Concentrations Largely Exceeded Those of Agricultural Topsoils Located in the Same Environmental Context. Therefore, Atmospheric Emissions from the Smelter Are Not the Only Cause of the High Contamination of the Woody Habitat Soils. This Last One Is Related to the Nature and the Contamination Level of Deposit in Relation with Human Activities (rubbles, Slag, Soils, Etc). with Regard to the Results Obtained with Chemical Extractions, the Mobility of Cd, Pb and Zn in These Soils Is Also Greater Than in Agricultural Soils. in the Forest, Pollutant Solubility Is Increased by Soil Acidic Ph. the Variability of the Physico-chemical Parameters and the High Metal Contamination of the Topsoils Are the Main Characteristics of the Woody Habitats Located around the Former Smelter. Although Never Taken into Account during Risk Assessment, the Disturbance of These Environmental Components Could Have Important Biogeochemical Impacts (nutrients and Metal Cycles). Moreover, Any Modification of the Soils' Use Could Potentially Cause Mobilization and Transfer of the Pollutants to the Biosphere. Six Years After the Closure of the Smelter, and As Social and Economic Pressures Considerably Increase in This Area, the Study of These Peculiar Ecosystems Is Necessary to Understand and Predict the Bioavailability, Transfer, Bioaccumulation and Effects of Pollutants in Food Chains. |
Auteurs : Lemière Sébastien; Grumiaux Fabien; Godet Jean-Philippe; Tanguy Marion; Delplace Patrick; Demuynck Sylvain; Brulle Franck; Pruvot Christelle; Vandenbulcke Franck; Douay Francis; Leprêtre Alain Utilisation de lombricides exposés en microcosms pour évaluer l’intérêt d’agents immobilisants pour la remediation de sols urbains contaminés par les métaux 2ème Rencontres Nationales de la Recherche sur les Sites & Sols Pollués, ADEME. 20-21 octobre 2009, Paris @conference{Lemiere2009, title = {Utilisation de lombricides exposés en microcosms pour évaluer l’intérêt d’agents immobilisants pour la remediation de sols urbains contaminés par les métaux}, author = {Sébastien Lemière and Fabien Grumiaux and Jean-Philippe Godet and Marion Tanguy and Patrick Delplace and Sylvain Demuynck and Franck Brulle and Christelle Pruvot and Franck Vandenbulcke and Francis Douay and Alain Leprêtre}, year = {2009}, date = {2009-01-01}, booktitle = {2ème Rencontres Nationales de la Recherche sur les Sites & Sols Pollués, ADEME. 20-21 octobre 2009, Paris}, note = {ACTN}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } |
Auteurs : Waterlot Christophe; Douay Francis The problem of arsenic interference in the analysis of Cd to evaluate its extractability in soils contaminated by arsenic Talanta, 80, pp. 716-7221 @article{Waterlot2009, title = {The problem of arsenic interference in the analysis of Cd to evaluate its extractability in soils contaminated by arsenic}, author = {Christophe Waterlot and Francis Douay}, year = {2009}, date = {2009-01-01}, journal = {Talanta}, volume = {80}, pages = {716-722}, abstract = {The arsenic (as) spectral interference observed in the determination of cadmium (cd) by inductively coupled plasma atomic emission spectrometry (icp-aes) was studied in atomic absorption spectrometry (aas) using flame (faas) and graphite furnace (gfaas) as atomizers. the soils of 15 kitchen gardens located near two smelters in the north of france were selected according the ratio as/cd. four different extracting solutions usually used to evaluate the mobility of cd were chosen to extract cd from these soil samples: citric acid 0.11 m, acetic acid 0.11 m, calcium chloride 0.01m and water. the quantitative determinations of cd in the 15 soils for each solvent were investigated by icp-aes at two lines (228.802 and 214.438 nm) and by faas or gfaas with two-way background compensation. compared to the cd concentrations measured in the acid solutions and in the cacl2 solution after the addition of a chemical modifier, it was clearly demonstrated that the high-speed self-reversal background compensation (hssr-method) was the method of choice to eliminate the spectral interference of as during cd determination by faas and by gfaas. in water, it was shown that the deuterium lamp used for the background compensation (d2-method) was able to eliminate the most of the as interference. in comparison with cd concentrations in water after adding a chemical modifier, those obtained with the hssr-method were similar and a very good correlation was obtained between these two methods (r2 = 0.995). it was therefore established that the hssr-method would be able to replace the chemical modifiers to eliminate as interference in the determination of cd-extractable from as contaminated soils.}, note = {ACL}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {article} } The arsenic (as) spectral interference observed in the determination of cadmium (cd) by inductively coupled plasma atomic emission spectrometry (icp-aes) was studied in atomic absorption spectrometry (aas) using flame (faas) and graphite furnace (gfaas) as atomizers. the soils of 15 kitchen gardens located near two smelters in the north of france were selected according the ratio as/cd. four different extracting solutions usually used to evaluate the mobility of cd were chosen to extract cd from these soil samples: citric acid 0.11 m, acetic acid 0.11 m, calcium chloride 0.01m and water. the quantitative determinations of cd in the 15 soils for each solvent were investigated by icp-aes at two lines (228.802 and 214.438 nm) and by faas or gfaas with two-way background compensation. compared to the cd concentrations measured in the acid solutions and in the cacl2 solution after the addition of a chemical modifier, it was clearly demonstrated that the high-speed self-reversal background compensation (hssr-method) was the method of choice to eliminate the spectral interference of as during cd determination by faas and by gfaas. in water, it was shown that the deuterium lamp used for the background compensation (d2-method) was able to eliminate the most of the as interference. in comparison with cd concentrations in water after adding a chemical modifier, those obtained with the hssr-method were similar and a very good correlation was obtained between these two methods (r2 = 0.995). it was therefore established that the hssr-method would be able to replace the chemical modifiers to eliminate as interference in the determination of cd-extractable from as contaminated soils. |
Auteurs : Waterlot Christophe; Pruvot Christelle; Douay Francis Évaluation d'une méthode de dosage du cadmium dans les solutions d’extraction de sols de potagers localisés aux alentours d’une ancienne fonderie de plomb 2ème Rencontres Nationales de la Recherche sur les Sites & Sols Pollués, ADEME. 20-21 octobre 2009, Paris @conference{Waterlot2009a, title = {Évaluation d'une méthode de dosage du cadmium dans les solutions d’extraction de sols de potagers localisés aux alentours d’une ancienne fonderie de plomb}, author = {Christophe Waterlot and Christelle Pruvot and Francis Douay}, year = {2009}, date = {2009-01-01}, booktitle = {2ème Rencontres Nationales de la Recherche sur les Sites & Sols Pollués, ADEME. 20-21 octobre 2009, Paris}, abstract = {Les activités passées de la fonderie de plomb metaleurop nord (1873 – 2003) située à noyelles- godault ont entraîné des rejets dans l’atmosphère de quantités importantes de poussières. ceux-ci ont engendré une forte contamination des sols aux alentours. des travaux antérieurs ont ainsi montré que les concentrations totales en cd, pb et zn dans les horizons labourés des sols agricoles du secteur excédaient fortement les teneurs agricoles habituelles régionales (sterckeman et al. 2000). dans une moindre mesure, il a aussi été constaté une contamination en ag, as, bi, cu, hg, in, ni, sb, se, sn et tl (sterckeman et al. 2002). plus récemment, douay et al. (2008) ont mis en évidence un degré de contamination en cd, pb et zn, mais aussi en bi, in, sb et as des sols urbains du secteur, supérieur à celui des sols agricoles situés dans le même contexte environnemental. ceci pourrait être expliqué par des remaniements et les pratiques des jardiniers, mais aussi par des apports en liaison avec l’utilisation du charbon pour les besoins domestiques. quels que soient leurs usages, les sols aux alentours de metaleurop nord présentent une contamination multi métallique pouvant engendrer des interférences lors du dosage de certains éléments. ainsi, les teneurs en cd mesurées peuvent différer selon les techniques analytiques utilisées. la détermination des teneurs en cd dans les solutions d’extraction en utilisant la spectrométrie d’émission atomique couplée à une torche à plasma (icp-aes : inductively coupled plasma – atomic emission spectrometry) peut présenter des inconvénients. ceux-ci sont particulièrement rencontrés lorsque les matrices digérées présentent des teneurs élevées en fe, en al, mais aussi en as, en comparaison avec celles en cd (lambkin and alloway 2000). c’est notamment le cas des sols aux alentours de metaleurop nord. par ailleurs, les longueurs d’onde 228,802 nm, 214,438 nm et 226,502 nm caractéristiques des raies d’émission de cd définissent celles relatives aux émissions secondaires respectivement de as, al et fe. des interférences spectrales causées par ces éléments au cours du dosage de cd peuvent donc engendrer une surévaluation ou une sous-évaluation des teneurs en cd mesurées (lambkin and alloway 2000). le dosage de cd par spectrométrie d’absorption atomique à la longueur d’onde caractéristique de la raie principale d’absorption de cet élément permet de s’affranchir des interférences spectrales causées par fe et al. cette technique de dosage élimine partiellement les interférences spectrales entre as et cd (lambkin and alloway 2000). en revanche, les caractéristiques des spectromètres d’absorption atomique (saa) ne permettent pas à ce jour de s’affranchir totalement des interférences spectrales entre ces deux éléments, et ceci en raison des longueurs d’onde d’absorption très proches qui les caractérisent (ıcd = 228,802 nm et ıas = 228,812 nm). la présentation porte sur l’évaluation d’une méthode de correction du bruit de fond généré par as au cours du dosage de cd dans les solutions d’extraction de sols urbains présentant des teneurs en as parfois supérieures à celles en cd. localisés à moins de 2 km de l’ancien site métallurgique metaleurop nord, les horizons labourés de 10 sols de potagers ont été échantillonnés. après séchage à une température inférieures à à 40°c, les terres ont été homogénéisées, puis broyées pour passer au travers d’un tamis de 315 μm. des extractions à l’eau ultra pure, au chlorure de calcium 0,01 m et à l’acide citrique 0,11 m ont été réalisées en triplicat sur chacun des échantillons composites. les teneurs en cd et en as des solutions obtenues ont été déterminées par icp-aes aux longueurs d’onde ıcd = 228,802 nm, ııcd = 214,438 nm et ıas = 193,696 nm. la démarche a été complétée par la détermination en saa de leurs teneurs en cd en utilisant deux modes de correction du bruit causée par l’espèce concomitante, as. le premier, appelé bgc-d2 (deuterium background correction) nécessite l’utilisation d’une lampe au deutérium. le second mode, dénommé bgc-sr (self reversal background correction), est basé sur l’utilisation d’une lampe cd à cathode creuse pulsée avec un courant dont l’intensité varie au cours du temps.}, note = {ACTN}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } Les activités passées de la fonderie de plomb metaleurop nord (1873 – 2003) située à noyelles- godault ont entraîné des rejets dans l’atmosphère de quantités importantes de poussières. ceux-ci ont engendré une forte contamination des sols aux alentours. des travaux antérieurs ont ainsi montré que les concentrations totales en cd, pb et zn dans les horizons labourés des sols agricoles du secteur excédaient fortement les teneurs agricoles habituelles régionales (sterckeman et al. 2000). dans une moindre mesure, il a aussi été constaté une contamination en ag, as, bi, cu, hg, in, ni, sb, se, sn et tl (sterckeman et al. 2002). plus récemment, douay et al. (2008) ont mis en évidence un degré de contamination en cd, pb et zn, mais aussi en bi, in, sb et as des sols urbains du secteur, supérieur à celui des sols agricoles situés dans le même contexte environnemental. ceci pourrait être expliqué par des remaniements et les pratiques des jardiniers, mais aussi par des apports en liaison avec l’utilisation du charbon pour les besoins domestiques. quels que soient leurs usages, les sols aux alentours de metaleurop nord présentent une contamination multi métallique pouvant engendrer des interférences lors du dosage de certains éléments. ainsi, les teneurs en cd mesurées peuvent différer selon les techniques analytiques utilisées. la détermination des teneurs en cd dans les solutions d’extraction en utilisant la spectrométrie d’émission atomique couplée à une torche à plasma (icp-aes : inductively coupled plasma – atomic emission spectrometry) peut présenter des inconvénients. ceux-ci sont particulièrement rencontrés lorsque les matrices digérées présentent des teneurs élevées en fe, en al, mais aussi en as, en comparaison avec celles en cd (lambkin and alloway 2000). c’est notamment le cas des sols aux alentours de metaleurop nord. par ailleurs, les longueurs d’onde 228,802 nm, 214,438 nm et 226,502 nm caractéristiques des raies d’émission de cd définissent celles relatives aux émissions secondaires respectivement de as, al et fe. des interférences spectrales causées par ces éléments au cours du dosage de cd peuvent donc engendrer une surévaluation ou une sous-évaluation des teneurs en cd mesurées (lambkin and alloway 2000). le dosage de cd par spectrométrie d’absorption atomique à la longueur d’onde caractéristique de la raie principale d’absorption de cet élément permet de s’affranchir des interférences spectrales causées par fe et al. cette technique de dosage élimine partiellement les interférences spectrales entre as et cd (lambkin and alloway 2000). en revanche, les caractéristiques des spectromètres d’absorption atomique (saa) ne permettent pas à ce jour de s’affranchir totalement des interférences spectrales entre ces deux éléments, et ceci en raison des longueurs d’onde d’absorption très proches qui les caractérisent (ıcd = 228,802 nm et ıas = 228,812 nm). la présentation porte sur l’évaluation d’une méthode de correction du bruit de fond généré par as au cours du dosage de cd dans les solutions d’extraction de sols urbains présentant des teneurs en as parfois supérieures à celles en cd. localisés à moins de 2 km de l’ancien site métallurgique metaleurop nord, les horizons labourés de 10 sols de potagers ont été échantillonnés. après séchage à une température inférieures à à 40°c, les terres ont été homogénéisées, puis broyées pour passer au travers d’un tamis de 315 μm. des extractions à l’eau ultra pure, au chlorure de calcium 0,01 m et à l’acide citrique 0,11 m ont été réalisées en triplicat sur chacun des échantillons composites. les teneurs en cd et en as des solutions obtenues ont été déterminées par icp-aes aux longueurs d’onde ıcd = 228,802 nm, ııcd = 214,438 nm et ıas = 193,696 nm. la démarche a été complétée par la détermination en saa de leurs teneurs en cd en utilisant deux modes de correction du bruit causée par l’espèce concomitante, as. le premier, appelé bgc-d2 (deuterium background correction) nécessite l’utilisation d’une lampe au deutérium. le second mode, dénommé bgc-sr (self reversal background correction), est basé sur l’utilisation d’une lampe cd à cathode creuse pulsée avec un courant dont l’intensité varie au cours du temps. |
Auteurs : Bidar Géraldine; Verdin Anthony; Garçon Guillaume; Pruvot Christelle; Laruelle Frédéric; Grandmougin-Ferjani Anne; Douay Francis; Shirali Pirouz Changes in fatty acid composition and content of two plants (Lolium perenne and Trifolium repens) grown during 6 and 18 months in a metal (Pb, Cd, Zn) contaminated field Water Air and Soil Pollution, 192(1-4), pp. 281-2911 @article{Bidar2008, title = {Changes in fatty acid composition and content of two plants (Lolium perenne and Trifolium repens) grown during 6 and 18 months in a metal (Pb, Cd, Zn) contaminated field}, author = {Géraldine Bidar and Anthony Verdin and Guillaume Garçon and Christelle Pruvot and Frédéric Laruelle and Anne Grandmougin-Ferjani and Francis Douay and Pirouz Shirali}, year = {2008}, date = {2008-01-01}, journal = {Water Air and Soil Pollution}, volume = {192}, number = {1-4}, pages = {281-291}, abstract = {The aim of this in situ study was to investigate the fatty acid (fa) composition and content in roots and shoots of lolium perenne and trifolium repens, grown under heavy metal stress (cd, pb, zn). the composition of fa was quite similar for the two plants and the two organs; main fa were palmitic acid (c16:0), oleic acid (c18:1), linoleic acid (c18:2) and linolenic acid (c18:3). for both plants, the major fa that characterized the roots was c18:2 whereas c18:3 was the prominent fa in shoots. for the first sampling (s1), in the roots of l. perenne and t. repens, polyunsaturated fatty acids (pufa) were affected by metal contamination while, in the second sampling (s2), pufa were affected in the shoots of the two plants. this alteration of pufa was well correlated with the bioaccumulation factor of metals which decreased in roots and increased in shoots with the time. moreover, a positive correlation was found between the pufa decrease and the malondialdehyde (mda) content, indicating the occurrence of a lipid peroxidation induced by the metal stress}, note = {ACL}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {article} } The aim of this in situ study was to investigate the fatty acid (fa) composition and content in roots and shoots of lolium perenne and trifolium repens, grown under heavy metal stress (cd, pb, zn). the composition of fa was quite similar for the two plants and the two organs; main fa were palmitic acid (c16:0), oleic acid (c18:1), linoleic acid (c18:2) and linolenic acid (c18:3). for both plants, the major fa that characterized the roots was c18:2 whereas c18:3 was the prominent fa in shoots. for the first sampling (s1), in the roots of l. perenne and t. repens, polyunsaturated fatty acids (pufa) were affected by metal contamination while, in the second sampling (s2), pufa were affected in the shoots of the two plants. this alteration of pufa was well correlated with the bioaccumulation factor of metals which decreased in roots and increased in shoots with the time. moreover, a positive correlation was found between the pufa decrease and the malondialdehyde (mda) content, indicating the occurrence of a lipid peroxidation induced by the metal stress |
Auteurs : Brulle Franck; Cocquerelle Claude; Mitta Guillaume; Castric Vincent; Douay Francis; Leprêtre Alain; Vandenbulcke Franck Identification and expression profile of gene transcripts differentially expressed during metallic exposure in Eisenia fetida coelomocytes Developmental and Comparative Immunology, 32, pp. 1441-14531 @article{Brulle2008, title = {Identification and expression profile of gene transcripts differentially expressed during metallic exposure in Eisenia fetida coelomocytes}, author = {Franck Brulle and Claude Cocquerelle and Guillaume Mitta and Vincent Castric and Francis Douay and Alain Leprêtre and Franck Vandenbulcke}, year = {2008}, date = {2008-01-01}, journal = {Developmental and Comparative Immunology}, volume = {32}, pages = {1441-1453}, abstract = {The aim of this work was to identify in eisenia fetida genes whose expression are regulated following exposure to a complex mixture of metallic trace elements (mte) representative of a highly polluted smelter soil. suppression subtractive hybridization (ssh) was used to construct cdna libraries enriched in up- or down-regulated transcripts in the immunecirculating cells of the coelomic cavities, namely coelomocytes, from worms exposed to metallic pollution. among 1536 ssh-derived cdna clones sequenced, we identified 764 unique ests of which we selected 18 candidates on the basis of their redundancy. these selected candidates were subjected to a two-step validation procedure based on the study of their expression level by real-time pcr. the first step consisted in measuring the expression of the 18 candidates in worms exposed to artificial contaminated soil. the second step consisted in measuring the expression in animals exposed to a ‘‘naturally’’ contaminated soil sampled close to a smelter. both steps allowed us to highlight 3 candidates that are strongly induced in worms exposed to a smelter polluted soil. these candidates are: the well-known mte-induced cd–metallothionein and 2 original biomarkers, lysenin, and a transcript, which cloning of the complete coding sequence identified as the coactosin-like protein.}, note = {ACL}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {article} } The aim of this work was to identify in eisenia fetida genes whose expression are regulated following exposure to a complex mixture of metallic trace elements (mte) representative of a highly polluted smelter soil. suppression subtractive hybridization (ssh) was used to construct cdna libraries enriched in up- or down-regulated transcripts in the immunecirculating cells of the coelomic cavities, namely coelomocytes, from worms exposed to metallic pollution. among 1536 ssh-derived cdna clones sequenced, we identified 764 unique ests of which we selected 18 candidates on the basis of their redundancy. these selected candidates were subjected to a two-step validation procedure based on the study of their expression level by real-time pcr. the first step consisted in measuring the expression of the 18 candidates in worms exposed to artificial contaminated soil. the second step consisted in measuring the expression in animals exposed to a ‘‘naturally’’ contaminated soil sampled close to a smelter. both steps allowed us to highlight 3 candidates that are strongly induced in worms exposed to a smelter polluted soil. these candidates are: the well-known mte-induced cd–metallothionein and 2 original biomarkers, lysenin, and a transcript, which cloning of the complete coding sequence identified as the coactosin-like protein. |
Auteurs : Brulle Franck; Cocquerelle Claude; Mitta Guillaume; Castric Vincent; Douay Francis; Leprêtre Alain; Vandenbulcke Franck Identification and expression profile of gene transcripts differentially expressed during metallic exposure in Eisenia fetida SETAC Europe 18th Annual Meeting, 25-29 May 2008, Krakow (Poland) @conference{Brulle2008b, title = {Identification and expression profile of gene transcripts differentially expressed during metallic exposure in Eisenia fetida}, author = {Franck Brulle and Claude Cocquerelle and Guillaume Mitta and Vincent Castric and Francis Douay and Alain Leprêtre and Franck Vandenbulcke}, year = {2008}, date = {2008-01-01}, booktitle = {SETAC Europe 18th Annual Meeting, 25-29 May 2008, Krakow (Poland)}, note = {ACTI}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } |
Auteurs : Douay Francis; Pruvot Christelle; Roussel Hélène; Ciesielski Henri; Fourrier Hervé; Proix Nicolas; Waterlot Christophe Contamination of urban soils in an area of Northern France polluted by dust emissions of two smelters Water Air and Soil Pollution, 188(1-4), pp. 247-2601 @article{Douay2008, title = {Contamination of urban soils in an area of Northern France polluted by dust emissions of two smelters}, author = {Francis Douay and Christelle Pruvot and Hélène Roussel and Henri Ciesielski and Hervé Fourrier and Nicolas Proix and Christophe Waterlot}, year = {2008}, date = {2008-01-01}, journal = {Water Air and Soil Pollution}, volume = {188}, number = {1-4}, pages = {247-260}, abstract = {The contamination of 27 urban topsoils has been assessed around two lead and zinc smelters (metaleurop nord and umicore) in the north of france. eighteen trace elements have been analysed (ag, as, bi, cd, co, cr, cu, hg, in, ni, pb, sb, se, sn, tl, th, u and zn). the investigation included the study of the vertical distribution of cd, pb and zn as indicators of pollution. it was shown that cd, in, pb, sb and zn were major pollutants followed in lesser quantities by ag, bi, cu and hg. in addition, as, ni, se, sn and tl were present at levels slightly higher than regional agricultural values. the other elements (co, cr, th and u) were at endogenous levels. the observations have highlighted the strong heterogeneity of the physico-chemical parameters of urban soils and the existence of heavy contamination of the under layers by cd, pb and zn. a potential transfer of metals from the topsoil to the deeper layers and especially cd and zn, is not excluded. indeed the soil rework is not the only factor explaining contamination level of the deeper layers of the studied soils. the comparison of the studied element concentrations in urban soils with nearby local agricultural values shows that the dust emission originating from the metaleurop and umicore smelters were not the only source of contamination. thus a large contamination of the studied urban soils by sb and in could be explained by domestic combustion of coal for heating.}, note = {ACL}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {article} } The contamination of 27 urban topsoils has been assessed around two lead and zinc smelters (metaleurop nord and umicore) in the north of france. eighteen trace elements have been analysed (ag, as, bi, cd, co, cr, cu, hg, in, ni, pb, sb, se, sn, tl, th, u and zn). the investigation included the study of the vertical distribution of cd, pb and zn as indicators of pollution. it was shown that cd, in, pb, sb and zn were major pollutants followed in lesser quantities by ag, bi, cu and hg. in addition, as, ni, se, sn and tl were present at levels slightly higher than regional agricultural values. the other elements (co, cr, th and u) were at endogenous levels. the observations have highlighted the strong heterogeneity of the physico-chemical parameters of urban soils and the existence of heavy contamination of the under layers by cd, pb and zn. a potential transfer of metals from the topsoil to the deeper layers and especially cd and zn, is not excluded. indeed the soil rework is not the only factor explaining contamination level of the deeper layers of the studied soils. the comparison of the studied element concentrations in urban soils with nearby local agricultural values shows that the dust emission originating from the metaleurop and umicore smelters were not the only source of contamination. thus a large contamination of the studied urban soils by sb and in could be explained by domestic combustion of coal for heating. |
Auteurs : Douay Francis; Roussel Hélène; Pruvot Christelle; Loriette Alexandre; Fourrier Hervé Assessment of a remediation technique using the replacement of contaminated soils in kitchen gardens nearby a former lead smelter in Northern France Science of the Total Environment, 401(1-3), pp. 29-381 @article{Douay2008a, title = {Assessment of a remediation technique using the replacement of contaminated soils in kitchen gardens nearby a former lead smelter in Northern France}, author = {Francis Douay and Hélène Roussel and Christelle Pruvot and Alexandre Loriette and Hervé Fourrier}, year = {2008}, date = {2008-01-01}, journal = {Science of the Total Environment}, volume = {401}, number = {1-3}, pages = {29-38}, abstract = {Vegetables cultivated in kitchen gardens that are strongly contaminated by heavy metals (pb, cd) may represent to consumers a means of exposure to these metals. this exposure is more problematic for those families that include a large quantity of home-grown vegetables in their diet. researchers have shown that the majority of vegetables produced in kitchen gardens in the vicinity of the metaleurop nord smelter (northern france) do not conform to european regulations. this study was carried out in three of these kitchen gardens. the concentrations of cd and pb in the topsoils were up to 24 and 3300 mg kg−1 respectively. the method consisted of delineating a surface area of about 50 to 100 m2 for each garden, then removing the contaminated soil and replacing it with a clean one. seven species of vegetables were cultivated from 2003 to 2005 in the original contaminated soils and the remediated ones. the data showed a clear improvement of the quality of the vegetables cultivated in remediated soils, although 17% of them were still over the european legislative limits for foodstuffs. this suggested that there was a foliar contamination due to contaminated dust fallout coming from the closed smelter site and the adjacent polluted soils. in addition, the measurement of the cd and pb concentrations in the dust fallout showed that the substantial rise in metal concentrations in the remediated soil was not only due to atmospheric fallout. these results raise questions about possible technical, economic and sociological problems associated with this kind of remediation.}, note = {ACL}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {article} } Vegetables cultivated in kitchen gardens that are strongly contaminated by heavy metals (pb, cd) may represent to consumers a means of exposure to these metals. this exposure is more problematic for those families that include a large quantity of home-grown vegetables in their diet. researchers have shown that the majority of vegetables produced in kitchen gardens in the vicinity of the metaleurop nord smelter (northern france) do not conform to european regulations. this study was carried out in three of these kitchen gardens. the concentrations of cd and pb in the topsoils were up to 24 and 3300 mg kg−1 respectively. the method consisted of delineating a surface area of about 50 to 100 m2 for each garden, then removing the contaminated soil and replacing it with a clean one. seven species of vegetables were cultivated from 2003 to 2005 in the original contaminated soils and the remediated ones. the data showed a clear improvement of the quality of the vegetables cultivated in remediated soils, although 17% of them were still over the european legislative limits for foodstuffs. this suggested that there was a foliar contamination due to contaminated dust fallout coming from the closed smelter site and the adjacent polluted soils. in addition, the measurement of the cd and pb concentrations in the dust fallout showed that the substantial rise in metal concentrations in the remediated soil was not only due to atmospheric fallout. these results raise questions about possible technical, economic and sociological problems associated with this kind of remediation. |
Auteurs : Douay Francis; Roussel Hélène; Pruvot Christelle; Waterlot Christophe Impact of a smelter closedown on metal contents of wheat cultivated in the neighbourhood Environmental Science and Pollution Research, 15, pp. 162-1691 @article{Douay2008b, title = {Impact of a smelter closedown on metal contents of wheat cultivated in the neighbourhood}, author = {Francis Douay and Hélène Roussel and Christelle Pruvot and Christophe Waterlot}, year = {2008}, date = {2008-01-01}, journal = {Environmental Science and Pollution Research}, volume = {15}, pages = {162-169}, abstract = {Background. the contamination of soils by heavy metals engenders important environmental and sanitary problems in northern france where a smelter has been located for more than one hundred of years. it has been one of the most important pb production sites in europe until its closedown in march 2003. ore smelting process generated considerable atmospheric emissions of dust. despite an active environmental strategy, these emissions were still significant in 2002 with up to 17 tonnes of pb, 32 tonnes of zn and 1 tonne of cd. over the years, the generated deposits have led to an important contamination of the surrounding soils. previous studies have shown pollutant transfers to plants, which can induce a risk for human and animal health. the objective of this study was to evaluate the consequences of the smelter closedown on the cd and pb contents of wheat (grain and straw) cultivated in the area. methods. paired topsoil and vegetable samples were taken at harvest time at various distances to the smelter. the sample sites were chosen in order to represent a large range of soil metal contamination. sampling was realised on several wheat harvests between 1997 and 2003. 25 samples were collected before the smelter closedown and 15 after. all ears of about 1 m long of two rows were manually picked and threshed in the lab. similarly, straw was harvested at the same time. total metal contents in soil and wheat samples were quantified. results. a negative correlation between metal concentrations in soil and the distance to the smelter was shown. the wheat grain and straw showed significant cd and pb contents. the straw had higher metal contents than the grain. during the smelter activity, the grain contents were up to 0.8 mg kg–1 dm of cd and 8 mg kg–1 dm of pb. for the straw, maximum contents were 5 mg kg–1 dm of cd and 114 mg kg–1 dm of pb. after the smelter closedown, we observed a very large decrease of pb in the grain (82%) and in the straw (91%). a smaller decrease was observed for cd in grain. despite this improvement, 80% of the studied samples remained non-acceptable for human consumption, according to the european legislation values, due to a high cd content. discussion. results highlighted a difference in metal accumulation in the plant organs as well as a difference in metal uptake. the approach pointed out the importance of atmospheric fallout in the wheat contamination pathways for pb. the smelter closedown has lead to a decrease of the pb content in wheat. it is interesting to relate this finding with the lead blood levels in children living close to the smelter. conclusions. those results have confirmed the importance of dust fallout in the plant contamination pathways. before the closedown, pb measured in the plant was principally originating from the smelter dust emissions. it raised the question of the sanitary risks for humans and animals living in the surrounding area of the smelter. recommendations and perspectives. in the literature, very few articles take the dust deposit as contamination pathways for crops into consideration. however, in highly contaminated sites, this pathway can be very important. thus, it would be worthy studying the uptake of metal contaminants by plants through the foliar system.}, note = {ACL}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {article} } Background. the contamination of soils by heavy metals engenders important environmental and sanitary problems in northern france where a smelter has been located for more than one hundred of years. it has been one of the most important pb production sites in europe until its closedown in march 2003. ore smelting process generated considerable atmospheric emissions of dust. despite an active environmental strategy, these emissions were still significant in 2002 with up to 17 tonnes of pb, 32 tonnes of zn and 1 tonne of cd. over the years, the generated deposits have led to an important contamination of the surrounding soils. previous studies have shown pollutant transfers to plants, which can induce a risk for human and animal health. the objective of this study was to evaluate the consequences of the smelter closedown on the cd and pb contents of wheat (grain and straw) cultivated in the area. methods. paired topsoil and vegetable samples were taken at harvest time at various distances to the smelter. the sample sites were chosen in order to represent a large range of soil metal contamination. sampling was realised on several wheat harvests between 1997 and 2003. 25 samples were collected before the smelter closedown and 15 after. all ears of about 1 m long of two rows were manually picked and threshed in the lab. similarly, straw was harvested at the same time. total metal contents in soil and wheat samples were quantified. results. a negative correlation between metal concentrations in soil and the distance to the smelter was shown. the wheat grain and straw showed significant cd and pb contents. the straw had higher metal contents than the grain. during the smelter activity, the grain contents were up to 0.8 mg kg–1 dm of cd and 8 mg kg–1 dm of pb. for the straw, maximum contents were 5 mg kg–1 dm of cd and 114 mg kg–1 dm of pb. after the smelter closedown, we observed a very large decrease of pb in the grain (82%) and in the straw (91%). a smaller decrease was observed for cd in grain. despite this improvement, 80% of the studied samples remained non-acceptable for human consumption, according to the european legislation values, due to a high cd content. discussion. results highlighted a difference in metal accumulation in the plant organs as well as a difference in metal uptake. the approach pointed out the importance of atmospheric fallout in the wheat contamination pathways for pb. the smelter closedown has lead to a decrease of the pb content in wheat. it is interesting to relate this finding with the lead blood levels in children living close to the smelter. conclusions. those results have confirmed the importance of dust fallout in the plant contamination pathways. before the closedown, pb measured in the plant was principally originating from the smelter dust emissions. it raised the question of the sanitary risks for humans and animals living in the surrounding area of the smelter. recommendations and perspectives. in the literature, very few articles take the dust deposit as contamination pathways for crops into consideration. however, in highly contaminated sites, this pathway can be very important. thus, it would be worthy studying the uptake of metal contaminants by plants through the foliar system. |
Auteurs : Gimbert Frédéric; De-Vaufleury Annette; Douay Francis; Coeurdassier Michaël; Scheifler Renaud; Badot Pierre-Marie Long-term responses of snails exposed to cadmium-contaminated soils in a partial life-cycle experiment Ecotoxicology and Environmental Safety, 70, pp. 138-1461 @article{Gimbert2008, title = {Long-term responses of snails exposed to cadmium-contaminated soils in a partial life-cycle experiment}, author = {Frédéric Gimbert and Annette De-Vaufleury and Francis Douay and Michaël Coeurdassier and Renaud Scheifler and Pierre-Marie Badot}, year = {2008}, date = {2008-01-01}, journal = {Ecotoxicology and Environmental Safety}, volume = {70}, pages = {138-146}, abstract = {Juvenile snails were exposed during their growth period to cd-contaminated field and artificial soils and then transferred to uncontaminated soil to assess the sequels of previous exposure on adult reproduction. growth modelling highlighted growth inhibitions of 5% and 10% after 70 and 84 days of exposure to 20 and 100mgcdkg(-1) in artificial soils, respectively. growth disruption was accompanied by a decrease in the clutch number and a 4-week delay in the egg-laying cycle. although it was also contaminated at 20mgcdkg(-1), the contaminated field soil did not lead to detectable effects in snails, suggesting a lower cd bioavailability confirmed by the bioaccumulation analysis. we demonstrated that the 28-day growth test, as advised by the iso-guideline, may not be sufficient to assess sublethal toxic effects of realistically contaminated soils. for this purpose, a life cycle experimental set-up is proposed, allowing a thorough assessment of toxicity during successive life stages.}, note = {ACL}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {article} } Juvenile snails were exposed during their growth period to cd-contaminated field and artificial soils and then transferred to uncontaminated soil to assess the sequels of previous exposure on adult reproduction. growth modelling highlighted growth inhibitions of 5% and 10% after 70 and 84 days of exposure to 20 and 100mgcdkg(-1) in artificial soils, respectively. growth disruption was accompanied by a decrease in the clutch number and a 4-week delay in the egg-laying cycle. although it was also contaminated at 20mgcdkg(-1), the contaminated field soil did not lead to detectable effects in snails, suggesting a lower cd bioavailability confirmed by the bioaccumulation analysis. we demonstrated that the 28-day growth test, as advised by the iso-guideline, may not be sufficient to assess sublethal toxic effects of realistically contaminated soils. for this purpose, a life cycle experimental set-up is proposed, allowing a thorough assessment of toxicity during successive life stages. |
Auteurs : Lemière Sébastien; Godet Jean-Philippe; Grumiaux Fabien; Delplace Patrick; Demuynck Sylvain; Vandenbulcke Franck; Douay Francis; Leprêtre Alain Eisenia fetida responses to metal immobilizing agents for contaminated soil remediation: a microcosm evaluation SETAC Europe 18th Annual Meeting. 25-29 May 2008, Krakow (Pologne) @conference{Lemiere2008, title = {Eisenia fetida responses to metal immobilizing agents for contaminated soil remediation: a microcosm evaluation}, author = {Sébastien Lemière and Jean-Philippe Godet and Fabien Grumiaux and Patrick Delplace and Sylvain Demuynck and Franck Vandenbulcke and Francis Douay and Alain Leprêtre}, year = {2008}, date = {2008-01-01}, booktitle = {SETAC Europe 18th Annual Meeting. 25-29 May 2008, Krakow (Pologne)}, note = {ACTI}, keywords = {Axe 2, ER4}, pubstate = {published}, tppubtype = {conference} } |