Membre

@article{leclercqdransart:hal-03767130,

title = {Comparison of the interest of four types of organic mulches to reclaim degraded areas (Part 2): Microbial activities and abiotic factors},

author = {Julie Leclercq-Dransart and Sylvain Demuynck and Fabien Grumiaux and Alain Leprêtre and Sébastien Lemière and Brice Louvel and Céline Pernin and Francis Douay},

url = {https://hal.archives-ouvertes.fr/hal-03767130},

doi = {10.1016/j.ecoleng.2022.106694},

year  = {2022},

date = {2022-09-01},

urldate = {2022-09-01},

journal = {Ecological Engineering},

volume = {182},

pages = {106694},

publisher = {Elsevier},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{courtois:hal-03149939,

title = {Medium-term effects of Ag supplied directly or via sewage sludge to an agricultural soil on Eisenia fetida earthworm and soil microbial communities},

author = {Pauline Courtois and Agnieszka Rorat and Sébastien Lemiere and Remy Guyoneaud and Eléonore Attard and Manon Longepierre and Franc cois Rigal and Clément Levard and Perrine Chaurand and Anna Grosser and Anna Grobelak and Malgorzata Kacprzak and Christine Lors and Agnes Richaume and Franck Vandenbulcke},

url = {https://hal.archives-ouvertes.fr/hal-03149939},

doi = {10.1016/j.chemosphere.2020.128761},

year  = {2021},

date = {2021-04-01},

urldate = {2021-04-01},

journal = {Chemosphere},

volume = {269},

pages = {128761},

publisher = {Elsevier},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Courtois2020,

title = {Accumulation, speciation and localization of silver nanoparticles in the earthworm Eisenia fetida},

author = {Pauline Courtois and Agnieszka Rorat and Sébastien Lemiere and Clément Levard and Perrine Chaurand and Anna Grobelak and Christine Lors and Franck Vandenbulcke},

year  = {2020},

date = {2020-01-01},

journal = {Environmental Science and Pollution Research},

pages = {10},

abstract = {The use of silver nanoparticles (AgNPs) in agriculture and many consumer 

	products has led to a significant release of Ag in the environment. 

	Although Ag toxicity in terrestrial organisms has been studied extensively, 

	very little is known about the accumulation capacity and coping mechanisms 

	of organisms in Ag-contaminated soil. In this context, we exposed 

	Eisenia fetida earthworms to artificial OECD soil spiked with a range 

	of concentrations of Ag (AgNPs or AgNO3). The main aims were to (1) 

	identify the location and form of accumulation of Ag in the exposed 

	earthworms and (2) better understand the physiological mechanisms 

	involved in Ag detoxification. The results showed that similar doses 

	of AgNPs or AgNO3 did not have the same effect on E. fetida survival. 

	The two forms of Ag added to soil exhibited substantial differences 

	in speciation at the end of exposure, but the Ag speciation and content 

	of Ag in earthworms were similar, suggesting that biotransformation 

	of Ag occurred. Finally, 3D images of intact earthworms obtained 

	by X-ray micro-computed tomography revealed that Ag accumulated preferentially 

	in the chloragogen tissue, coelomocytes, and nephridial epithelium. 

	Thus, E. fetida bioaccumulates Ag, but a regulation mechanism limits 

	its impact in a very efficient manner. The location of Ag in the 

	organism, the competition between Ag and Cu, and the speciation of 

	internal Ag suggest a link between Ag and the thiol-rich proteins 

	that are widely present in these tissues, most probably metallothioneins, 

	which are key proteins in the sequestration and detoxification of 

	metals.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Slaby2020,

title = {Effects of glyphosate and a commercial formulation Roundup (R) exposures on maturation of Xenopus laevis oocytes},

author = {Sylvain Slaby and Pauline Titran and Guillaume Marchand and Julie Hanotel and Arlette Lescuyer and Alain Leprêtre and Jean-François Bodart and Matthieu Marin and Sébastien Lemiere},

year  = {2020},

date = {2020-01-01},

journal = {Environmental Science and Pollution Research},

volume = {27},

number = {4},

pages = {3697-3705},

abstract = {Pesticides are often found at high concentrations in small ponds near 

	agricultural field where amphibians are used to live and reproduce. 

	Even if there are many studies on the impacts of phytopharmaceutical 

	active ingredients in amphibian toxicology, only a few are interested 

	in the earlier steps of their life cycle. While their populations 

	are highly threatened with extinction. The aim of this work is to 

	characterize the effects of glyphosate and its commercial formulation 

	Roundup (R) GT Max on the Xenopus laevis oocyte maturation which 

	is an essential preparation for the laying and the fertilization. 

	Glyphosate is an extensively used herbicide, not only known for its 

	effectiveness but also for its indirect impacts on non-target organisms. 

	Our results showed that exposures to both forms of glyphosate delayed 

	this hormone-dependent process and were responsible for spontaneous 

	maturation. Severe and particular morphogenesis abnormalities of 

	the meiotic spindle were also observed. The MAPK pathway and the 

	MPF did not seem to be affected by exposures. The xenopus oocyte 

	is particularly affected by the exposures and appears as a relevant 

	model for assessing the effects of environmental contamination.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Lanier2019,

title = {Combined toxic effects and DNA damage to two plant species exposed to binary metal mixtures (Cd/Pb)},

author = {Caroline Lanier and Fabien Bernard and Sylvain Dumez and Julie Leclercq-Dransart and Sébastien Lemière and Franck Vandenbulcke and Fabrice Nesslany and Anne Platel and Inès Devred and Audrey Hayet and Damien Cuny and Annabelle Deram},

year  = {2019},

date = {2019-01-01},

journal = {Ecotoxicology and Environmental Safety},

volume = {167},

pages = {278-287},

abstract = {Acute and long-term (3-, 10- and 56-day exposure) laboratory toxicity 

	tests were carried out to assess the individual and combined toxic 

	effects of cadmium (Cd) and lead (Pb) in Brassica oleracea and Trifolium 

	repens. In addition to morphological parameters, this work also used 

	comet assay to address endpoints in relation to genotoxicity. Bioaccumulation 

	was measured to demonstrate the influence of the mixture on the concentrations 

	of each metal in the plant. 

 

	The statistical method reported by Ince et al. (1999) was used to 

	evaluate the types of interaction between Cd and Pb in each treatment 

	and concerning their combined effect. 

 

	This study concludes that the combined effects of binary metal combinations 

	of Cd/Pb on morphological parameters are most often additive, sometimes 

	antagonistic and more rarely synergistic, thus extending the findings 

	of previous publications on this subject. 

 

	DNA damage analysis revealed concentration- and time-dependent interactions. 

	Synergistic effects of mixed metals (more breaks than individually 

	applied metals) are observed in T. repens after a short exposure. 

	Antagonistic effects are statistically significant after 10 days-exposure, 

	suggesting competition between metals. At 56 days, the rate of DNA 

	damage observed in plants exposed to the Cd/Pb mixture was similar 

	to that measured in plants exposed to lead only and was significantly 

	lower than the rate of DNA damage induced by Cd. This supports the 

	idea that there may be competition between metals and also strengthens 

	the hypothesis that long-term reparation mechanisms may be implemented. 

	Cd/Pb co-exposure does not significantly influence the bioaccumulation 

	of each metal. It is nevertheless important to note that a statistically 

	significant ‘interaction’ is not necessarily biologically relevant 

	and should therefore be considered with caution when assessing heavy 

	metals combined effects},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Leclercq-Dransart2019b,

title = {Isopod physiological and behavioral responses to drier conditions: An experiment with four species in the context of global warming},

author = {Julie Leclercq-Dransart and Céline Pernin and Sylvain Demuynck and Fabien Grumiaux and Sébastien Lemière and Alain Leprêtre},

year  = {2019},

date = {2019-01-01},

journal = {European Journal of Soil Biology},

volume = {90},

pages = {22-30},

abstract = {In the context of global warming, an increase in soil drought is suspected 

	by the IPCC predictions and litter breakdown activity could be affected. 

	An experiment was conducted using microcosms (controlled conditions) 

	and woodlice which are recognized as shredders of litter and bioindicators 

	of global warming. The impact of relative air humidity (90 and 50% 

	RH) on litter breakdown by woodlice was studied through the monitoring 

	of one behavioral (distribution of individuals on microcosms), one 

	morphological (Absolute Growth Rate) and four physiological traits 

	(Survival rate, Relative Consumption Rate, Relative Growth Rate, 

	Feeding rate). Four species of isopods known to have different microclimatic 

	sensitivities (Armadillidium vulgare, Porcellio scaber, Oniscus asellus, 

	Philoscia muscorum) were used simultaneously. Sensitivities of males 

	and females have also been tested. Main results showed that the seven 

	studied parameters under the two relative humidity conditions were 

	not affected in the same way according to the species nor genders. 

	A. vulgare is the most affected species followed by O. asellus. The 

	least affected species are P. scaber and P. muscorum. Their morphological 

	and physiological differences allow to the most evolved species to 

	be more resistant to drier conditions but in return they are no longer 

	active, which can affect soil functionality. With global warming, 

	it could be envisaged that woodlice spend less time foraging and 

	more time seeking refuge to reduce the risk of mortality from desiccation 

	stress. Modifying the feeding behavior of terrestrial isopods could 

	slow down litter degradation with consequences on the process of 

	organic matter recycling.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Marchand2019,

title = {Adverse effects of fly ashes used as immobilizing agents for highly metal-contaminated soils on Xenopus laevis oocytes survival and maturation-a study performed in the north of France with field soil extracts},

author = {Guillaume Marchand and Sylvain Demuynck and Sylvain Slaby and Arlette Lescuyer and Sébastien Lemière and Matthieu Marin},

year  = {2019},

date = {2019-01-01},

journal = {Environmental Science and Pollution Research},

volume = {27},

pages = {3706–3714},

abstract = {Amphibians are now recognized as the most endangered group. One of 

	this decline causes is the degradation of their habitat through direct 

	contamination of water, soil leaching, or runoff from surrounding 

	contaminated soils and environments. In the North of France, the 

	extensive industrial activities resulted in massive soil contamination 

	by metal compounds. Mineral amendments were added to soils to decrease 

	trace metal mobility. Because of the large areas to be treated, the 

	use of inexpensive industrial by-products was favored. Two types 

	of fly ashes were both tested in an experimental site with the plantation 

	of trees in 2000. Aim of the present work was to investigate the 

	effects of extracts from metal-contaminated soils treated or not 

	for 10 years with fly ashes on Xenopus laevis oocyte using cell biology 

	approaches. Indeed, our previous studies have shown that the Xenopus 

	oocyte is a relevant model to study the metal ion toxicity. Survival 

	and maturation of oocyte exposed to the soil extracts were evaluated 

	by phenotypic approaches and electrophysiological recordings. An 

	extract derived from a metal-contaminated soil treated for 10 years 

	with sulfo-calcic ashes induced the largest effects. Membrane integrity 

	appeared affected and ion fluxes in exposed oocytes were changed. 

	Thus, it appeared that extracted elements from certain mineral amendments 

	used to prevent the mobility of metals in the case of highly metal-contaminated 

	soils could have a negative impact on X. laevis oocytes.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Courtois2019,

title = {Ecotoxicology of silver nanoparticles and their derivatives introduced in soil with or without sewage sludge: A review of effects on microorganisms, plants and animals},

author = {Pauline Courtois and Agnieszka Rorat and Sébastien Lemiere and Remy Guyoneaud and Eleonore Attard and Clément Levard and Franck Vandenbulcke},

year  = {2019},

date = {2019-01-01},

journal = {Environmental Pollution},

volume = {253},

pages = {578-598},

abstract = {Silver nanoparticles (AgNPs) are widely incorporated in many products, 

	partly due to their antimicrobial properties. The subsequent discharge 

	of this form of silver into wastewater leads to an accumulation of 

	silver species (AgNPs and derivatives resulting from their chemical 

	transformation), in sewage sludge. As a result of the land application 

	of sewage sludge for agricultural or remediation purposes, soils 

	are the primary receiver media of silver contamination. Research 

	on the long-term impact of AgNPs on the environment is ongoing, and 

	this paper is the first review that summarizes the existing state 

	of scientific knowledge on the potential impact of silver species 

	introduced into the soil via sewage sludge, from microorganisms to 

	earthworms and plants. Silver species can easily enter cells through 

	biological membranes and affect the physiology of organisms, resulting 

	in toxic effects. In soils, exposure to AgNPs may change microbial 

	biomass and diversity, decrease plant growth and inhibit soil invertebrate 

	reproduction. Physiological, biochemical and molecular effects have 

	been documented in various soil organisms and microorganisms. Negative 

	effects on organisms of the dominant form of silver in sewage sludge, 

	silver sulfide (Ag2S), have been observed, although these effects 

	are attenuated compared to the effects of metallic AgNPs. However, 

	silver toxicity is complex to evaluate and much remains unknown about 

	the ecotoxicology of silver species in soils, especially with respect 

	to the possibility of transfer along the trophic chain via accumulation 

	in plant and animal tissues. Critical points related to the hazards 

	associated with the presence of silver species in the environment 

	are described, and important issues concerning the ecotoxicity of 

	sewage sludge applied to soil are discussed to highlight gaps in 

	existing scientific knowledge and essential research directions for 

	improving risk assessment. (C) 2019 Elsevier Ltd. All rights reserved.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Arguelles2017,

title = {Studies on the potential of new biomolecules for the protection of plants in organic agriculture: PHYTOBIO and SMARTBIOCONTROL projects. - Etudes des potentialites de nouvelles biomolecules pour la protection des plantes en agriculture biologique les projets PHYTOBIO et SMARTBIOCONTROL},

author = {A Arguelles and François Krier and François Coutte and Valérie Leclère and Sébastien Lemière and Monica Höfte and Marc Ongena and Essaid-Ait Barka and Stephan Dorey and Cédric Jacquard and N G Khong and Benoit Tisserant and Béatrice Randoux and Philippe Reignault and Lieven Delanote and Nathalie Cap and Philippe Jacques},

year  = {2017},

date = {2017-01-01},

booktitle = {6ème conférence internationale sur les méthodes alternatives de protection des plantes, 21-23 mars 2017, Lille},

note = {ACTN},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Courtois2017,

title = {Sewage sludge, silver nanoparticles and terrestrial environments: state of the art and involved work},

author = {Pauline Courtois and Agnieszka Rorat and Sébastien Lemière and Franck Vandenbulcke},

year  = {2017},

date = {2017-01-01},

booktitle = {International Conference of Environmental Biotechnology, 12th December 2017, Czestochowa (Poland)},

abstract = {The fast increase of the global population, urbanization, industrialization and the improvement of technologies used in wastewater treatment plant have led to a strong increase of the production of sewage sludge. because of the high organic matter and nutrients content, sewage sludge can be applied as a fertilizer, but some contaminants shall be strictly monitored. in europe, legislation focused on: seven metal trace elements (cadmium, chromium, copper, mercury, nickel, lead and zinc) and organic traces compounds (7 polychlobiphenyls and 3 polycyclic aromatic hydrocarbons (fluoranthene, benzo(b)fluoranthene and benzo(a)pyrene)). still, some potentially dangerous contaminants, e.g. silver nanoparticles are not regulated at present. due to their biocidal properties, silver nanoparticles are among the most frequently used nanomaterials (in more of 300 products and production estimated around 500 tons per year). releases in the environment through wastewater treatment plants, linked to the life cycle of products containing nano-silver, can cause a contamination of ecosystem and ecotoxicological and/or health effects [1]. many research aimed on the assessment of the risks and dangers associated with the use of silver, nano-silver and their derivatives on organisms and ecosystems. recent works have shown that the nano-silver 1) decrease the diversity and the quantity of soil microorganisms [2], 2) are accumulated in plant and animal tissues [3,4] and 3) cause biological effects on studied organisms. nevertheless, the understanding of their effects on the terrestrial ecosystem is still incomplete and many questions subsist. this work aims on the effects of the nano-silver toxicity in terrestrial environment, mainly throught its effects on organisms living in close contact with soil, to characterize it and to understand its mechanisms of action on organisms. references [1] anses, 2015. avis de l’agence nationale de sécurité sanitaire de l’alimentation, de l’environnement et du travail. [2] schlich k.et hund-rinke, k., 2015. influence of soil properties on the effect of silver nanomaterials on microbial activity in five soils. environmental pollution. 196, 321–330. [3] colman b.p., arnaout c.l., anciaux s., gunsch c.k., hochella jr m.f., kim b., lowry g.v., mcgill b.m., reinsch b.c., c.j. richardson, unrine j.m., wright j.p., yin l. et bernhardt e.s. 2013. low concentrations of silver nanoparticles in biosolids cause adverse ecosystem responses under realistic field scenario. plos one 8(2): e57189. doi: 10.1371/journal.pone.0057189 [4] shoults-wilson, w.a., reinsch, b.c., tsyusko, o.v., bertsch, p.m., lowry, g.v., unrine, j.m., 2011. effect of silver nanoparticle surface coating on bioaccumulation and reproductive toxicity in earthworms ( eisenia fetida ). nanotoxicology 5, 432–444.},

note = {COM},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Kondratskyi2017,

title = {Ferroquine, the next generation antimalarial drug, has antitumor activity},

author = {Artem Kondratskyi and Kateryna Kondratska and Fabien Vanden-Abeele and Dmitri Gordienko and Charlotte Dubois and Robert-Allain Toillon and Christian Slomianny and Sébastien Lemière and Philippe Delcourt and Etienne Dewailly and Roman Skryma and Christophe Biot and Natalia Prevarskaya},

year  = {2017},

date = {2017-01-01},

journal = {Scientific Reports},

volume = {7},

pages = {online},

abstract = {Despite the tremendous progress in medicine, cancer remains one of the most serious global health problems awaiting new effective therapies. here we present ferroquine (fq), the next generation antimalarial drug, as a promising candidate for repositioning as cancer therapeutics. we report that fq potently inhibits autophagy, perturbs lysosomal function and impairs prostate tumor growth in vivo. we demonstrate that fq negatively regulates akt kinase and hypoxia-inducible factor-1α (hif-1α) and is particularly effective in starved and hypoxic conditions frequently observed in advanced solid cancers. fq enhances the anticancer activity of several chemotherapeutics suggesting its potential application as an adjuvant to existing anticancer therapy. alike its parent compound chloroquine (cq), fq accumulates within and deacidifies lysosomes. further, fq induces lysosomal membrane permeabilization, mitochondrial depolarization and caspase-independent cancer cell death. overall, our work identifies ferroquine as a promising new drug with a potent anticancer activity.},

note = {ACLO},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Lemiere2017,

title = {Soil environmental genotoxicity: past and actual tools, future needs},

author = {Sébastien Lemière},

year  = {2017},

date = {2017-01-01},

booktitle = {International Conference of Environmental Biotechnology, 12 December 2017, Czestochowa (Poland)},

note = {ACTI},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Lemiere2017a,

title = {Soil environmental genotoxicity: past and actual tools, future needs},

author = {Sébastien Lemière and Paule Vasseur},

year  = {2017},

date = {2017-01-01},

booktitle = {18th International Symposium on Toxicity Assesment, 16- 21 July 2017, Limeira (Brazil)},

abstract = {It is now established that risk assessment and management strategies for contaminated environments should consider biological responses, including environmental genotoxicity. the characterization of terrestrial ecosystem contamination by genotoxicants requires to assess their environmental and toxicological bioavailabilities and to identify adverse effects at the individual and population levels. genotoxicity in field species is assessed by means of markers focusing on genomic alterations and dna damage, like dna adducts (less used lately), micronuclei and nuclear abnormalities and dna breaks. epigenetic changes are not investigated so far. the comet assay (with the use or not of endonucleases) has nowadays largely supplanted pre-existing techniques for dna damage assessment. all these tools/techniques have first been used, optimized and validated with aquatic species. their application to soil species has emerged in a second step and is now widespread. we will present briefly some results illustrating interests of the comet assay to approach the “genotoxic bioavailability” of terrestrial contaminants and their effects on earthworms. in both aquatic and terrestrial environments, genotoxicity is generally measured in somatic cells. in some cases, it could be related to mutations, embryo mortality or reproduction impairment. however, there is a need for works linking (1) genotoxicity in individuals and (2) population dynamics and genetics. studies with gametes, early life stages and juveniles, and in environmentally relevant conditions should be conducted to reach such objectives.},

note = {ACTI},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Rorat2017b,

title = {ETNA2 project: Silver ecotoxicology in terrestrial environments},

author = {Agnieszka Rorat and Pauline Courtois and Sébastien Lemière and E Attard and Rémy Guyoneaud and C Levard and Franck Vandenbulcke},

year  = {2017},

date = {2017-01-01},

booktitle = {International Conference of Environmental Biotechnology, 12th December 2017, Czestochowa (Poland)},

abstract = {Due to the increasing incorporation of silver nanoparticles (agnp) in consumer products, silver contamination of sewage sludge may increase and have environmental impacts. indeed sewage sludge are sometimes used in agriculture as fertilizers. in such a context, our project etna2 propose to monitor the silver concentrations in selected sewage sludge coming from southern poland during 2 years and examine their evolution in term of concentrations. in parallel, the assessment of the terrestrial ecotoxicity of silver ions, silver nanoparticles (agnp) and their transformation products (sulphides of silver) will be conducted. therefore, such ecotoxicological assessment will be done using key organisms of soil functioning which are affected by chemical contamination: microorganisms and earthworms. exposures of the ecotoxicologically important test species eisenia fetida were conducted in mesocosms containing agricultural reference soil contaminated by sewage sludge, ag ions, agnp or silver transformation products. these compounds were added to the soil or through the sludge after fermentation. our aim is to address the lack of informations concerning the question of silver in soil ecotoxicology. the impact of ag on soil microflora communities was explored as preliminary experiments evidenced the bactericidal properties of ag in this environmental context. a better understanding of the effects of ag, depending on its form (sulphides from wwtp sludge, ag ions and agnps) will be achieved mainly through genomic and trancriptomic approaches combined with full physico-chemical characterization. analysis will be conducted to make a link between silver evolution/ behavior in sludge and soils and their terrestrial ecotoxicity},

note = {COM},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Rorat2017c,

title = {Silver and silver nanoparticles as a threat for terrestrial ecosystems},

author = {Agnieszka Rorat and Pauline Courtois and Sébastien Lemière and Franck Vandenbulcke},

year  = {2017},

date = {2017-01-01},

booktitle = {The XXVI International Symposium “Molecular and Physiological Aspects of Regulatory Processes of the Organism, 16 September 2017, Kraków (Poland)},

note = {AFF},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Slaby2017,

title = {Maturation of Xenopus laevis oocytes under cadmium and lead exposures: cell biology investigations},

author = {Sylvain Slaby and Julie Hanotel and Guillaume Marchand and Arlette Lescuyer and Jean-François Bodart and Alain Leprêtre and Sébastien Lemière and Matthieu Marin},

year  = {2017},

date = {2017-01-01},

journal = {Aquatic Toxicology},

volume = {193},

pages = {105-110},

abstract = {Since amphibians are recognised as good models to assess the quality of environments, only few studies have dealt with the impacts of chemical contaminants on their gametes, while toxic effects at this stage will alter all the next steps of their life cycle. therefore, we propose to investigate the oocyte maturation of xenopus laevis in cadmium- and lead-contaminated conditions. the impacts of cadmium and lead ions were explored on events involved in the hormone‐dependent process of maturation. in time-course experiments, cadmium, at the highest concentration, delayed and prevented the germinal vesicle breakdown. even in the absence of progesterone this ion could also induce it. no such spontaneous maturation was observed after lead exposures. an acceleration of the process at the highest tested concentration of lead (90 μm), in presence of progesterone, was recorded. cytological observations highlighted that cadmium exposures drove severe disturbances of meiotic spindle morphogenesis. at last, cadmium exposures altered the mapk pathway, regarding the activation of erk2 and rsk, but also the activation and the activity of the mpf, by disturbing the state of phosphorylation of cdc2 and histone h3. xenopus laevis oocytes were affected by these metal ion exposures, notably by cd2+. signatures of these metal exposures on the oocyte maturation were detected. this germ cell appeared to be a relevant model to assess the effects of environmental contaminants such as metals.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Slaby2017a,

title = {Xenopus oocyte responses after cadmium and lead exposures: a cell biology investigation},

author = {Sylvain Slaby and Julie Hanotel and Guillaume Marchand and Arlette Lescuyer and Jean-François Bodart and Alain Leprêtre and Sébastien Lemière and Matthieu Marin},

year  = {2017},

date = {2017-01-01},

booktitle = {SETAC, 7-11 May 2017, Brussels (Belgique)},

abstract = {Cadmium and lead are recognized as priority contaminants to be monitored in the environment. they are toxic (behavioural, morphological, cellular and biochemical disturbances) for numerous organisms. amphibians are especially threatened by contaminant exposures, whatever the stage of their life cycle. our previous studies demonstrated that cd, but not pb, seriously impacted different aspects of the xenopus laevis’ oocyte maturation. from these observations, we aim to highlight specific targets of metal contaminant exposures in the xenopus oocyte. thus, (1) meiosis resumption kinetic studies, (2) analysis of meiotic spindle formation and (3) immuno-detection of some major biochemical actors of the maturation process (mitogen activated protein kinase pathway, mapk, and m-phase promoting factor, mpf) were conducted. the same concentrations tested in previous studies (0 to 25 mg.l-1 of metal chlorides) were used. for cd, (1) we observed only for 25 mg.l-1, that the germinal vesical breakdown (gvbd) was slowed down and also non hormono-induced maturations. (2) ectopic and/or abnormal spindles as well as disorganised chromosomes were present for all exposure concentrations (0.25 to 25 mg.l-1). (3) no deregulation in the mapk pathway was engendered, but disturbances in the mpf pathway were reported. this last result could be related to the cytological observations. concerning pb, (1) gvbd kinetics showed contrasted profiles. it seemed that a delay occurred at the end (since 720 min) for 0.25 mg.l-1. in contrast, for 25 mg.l 1, a transitory acceleration was recorded during the maturation. no other effect in term of cytological and biochemical analysis (2 and 3) were observed for pb exposures. from all these results, we can conclude that cd and pb did not affect the same cellular targets and could have specific exposure signatures. this study, in addition to our previous ones, contributed to better understand the effects of these metal contaminants in the first stages of the amphibian life cycle: oocyte and embryo. with similar approaches, works with other contaminants are in progress.},

note = {AFF},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Slaby2017b,

title = {Intérêt de l'ovocyte de xénope en écotoxicologie},

author = {Sylvain Slaby and Arlette Lescuyer and Guillaume Marchand and Alain Leprêtre and Jean-François Bodart and Matthieu Marin and Sébastien Lemière},

year  = {2017},

date = {2017-01-01},

booktitle = {Société Française d'Ecotoxicologie Fondamentale et Appliquée, 29-30 juin 2017, Lille},

note = {ACTN},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Slaby2017c,

title = {Xenopus oocytes in environmental toxicology: a promising tool ?},

author = {Sylvain Slaby and Arlette Lescuyer and Guillaume Marchand and Alain Leprêtre and Jean-François Bodart and Matthieu Marin and Sébastien Lemière},

year  = {2017},

date = {2017-01-01},

booktitle = {18th International Symposium on Toxicity Assesment, 16- 21 July 2017, Limeira (Brazil)},

abstract = {Amphibians stand for organisms of choice to assess toxicity. for example, the fetax test (frog embryo teratogenesis assay – xenopus) allows quick testing of chemicals with endpoints such as survival, growth and malformations of tadpoles. we develop complementary methods in amphibian toxicology, using xenopus laevis oocytes and young tadpoles as biological models. indeed the xenopus oocytes present many advantages: they are easy to handle and could be obtained in large numbers. their life cycle is well known and all the cells were synchronous. so different types of toxicity endpoints can be assessed with these gametes, regarding key moments of their cycle when conducted in contaminated environments. in a same way, contaminant exposures on fertilization and the precocious embryonic development can be performed. we will present the different kinds of data that can be obtained from maturation and survival ratios, analysis of meiotic spindle formation, to physiological data or automatic biometric measurements on tadpoles. several exposures have been conducted using environmental contaminants as metals (lead, cadmium) and pesticides (glyphosate, deltamethrin) and obtained results will be presented. analyzing early stages procured complementary information to preexisting methods. moreover oocytes seem to be particularly impacted in case of some exposures and suggest that they could be considered as a pertinent model to assess environmental contaminants effects.},

note = {ACTI},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Leclercq-Dransart2017,

title = {Agir sur la « Trame brune » pour favoriser la mobilité de la faune du sol},

author = {Julie Leclercq-Dransart and Sylvain Demuynck and Fabien Grumiaux and Sébastien Lemière and Céline Pernin and Alain Leprêtre},

year  = {2017},

date = {2017-01-01},

booktitle = {Journée de lancement du programme CPER Climibio, 6 septembre 2017, Lille},

note = {AFF},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Leclercq-Dransart2017a,

title = {Les isopodes terrestres face au dérèglement climatique},

author = {Julie Leclercq-Dransart and Sylvain Demuynck and Fabien Grumiaux and Sébastien Lemière and Céline Pernin and Alain Leprêtre},

year  = {2017},

date = {2017-01-01},

booktitle = {6ème journée du groupe de travail « TEBIS » (Traits Ecologiques et Biologiques des organismes des Sols), 2-4 octobre 2017, Metz},

note = {COM},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Leclercq-Dransart2017b,

title = {Les isopodes terrestres face aux changements climatiques},

author = {Julie Leclercq-Dransart and Sylvain Demuynck and Fabien Grumiaux and Sébastien Lemière and Céline Pernin and Alain Leprêtre},

year  = {2017},

date = {2017-01-01},

booktitle = {Journée de lancement du programme CPER Climibio, 6 septembre 2017, Lille},

note = {AFF},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Bernard2016,

title = {Antioxidant defense gene analysis in Brassica oleracea and Trifolium repens exposed to Cd and/or Pb},

author = {Fabien Bernard and Sylvain Dumez and Franck Brulle and Sébastien Lemière and Anne Platel and Fabrice Nesslany and Damien Cuny and Annabelle Deram and Franck Vandenbulcke},

year  = {2016},

date = {2016-01-01},

journal = {Environmental Science and Pollution Research},

volume = {23},

number = {4},

pages = {3136-3151},

abstract = {This study focused on the expression analysis of antioxidant defense genes in brassica oleracea and in trifolium repens. plants were exposed for 3, 10, and 56 days in microcosms to a field-collected suburban soil spiked by low concentrations of cadmium and/or lead. in both species, metal accumulations and expression levels of genes encoding proteins involved and/or related to antioxidant defense systems (glutathione transferases, peroxidases, catalases, metallothioneins) were quantified in leaves in order to better understand the detoxification processes involved following exposure to metals. it appeared that strongest gene expression variations in t. repens were observed when plants are exposed to cd (metallothionein and ascorbate peroxidase upregulations) whereas strongest variations in b. oleracea were observed in case of cd/pb co-exposures (metallothionein, glutathione transferase, and peroxidase upregulations). results also suggest that there is a benefit to use complementary species in order to better apprehend the biological effects in ecotoxicology},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Demuynck2016,

title = {Comparative avoidance behaviour of the earthworm Eisenia fetida towards chloride, nitrate and sulphate salts of Cd, Cu and Zn using filter paper and extruded water agar gels as exposure media},

author = {Sylvain Demuynck and Aurélie Lebel and Fabien Grumiaux and Céline Pernin and Alain Leprêtre and Sébastien Lemière},

year  = {2016},

date = {2016-01-01},

journal = {Ecotoxicology and Environmental Safety},

volume = {129},

pages = {66-74},

abstract = {We studied the avoidance behaviour of the earthworm eisenia fetida towards cd, cu, and zn, trace elements (tes) tested as chloride, nitrate and sulphate salts. sub adults were exposed individually using dual-cell chambers at 20+2 degrees c in the dark. recordings were realised at different dates from 2 h to 32 h. we used filter paper and extruded water agar gel as exposure media to evaluate the contribution of the dermal and the digestive exposure routes on the avoidance reactions. exposures to cu or cd (10 mg metal ion l-1) resulted in highly significant avoidance reactions through the exposure duration. worms avoided zn poorly and reactions towards zn salts varied along the exposure. worm sensitivity towards tes differed between salts and this could result from differential toxicity or accessibility of these te salts to earthworms. the anion in itself was not the determinant of the avoidance reactions since exposures to similar concentrations of these anions using calcium salts did not result in significant avoidance worm behaviour. avoidance responses towards tes were higher in the case of water agar exposures than in filter paper exposures. thus, dermal contacts with te solutions would elicit worm avoidance but signals from receptors located inside the digestive tract could reinforce this behaviour. the use of extruded water agar gels as the substrate allows checking the real sensitivity of earthworm species towards tes since the te concentrations leading to significant avoidance reactions were below those reported in the literature when using te-spiked soils. (c) 2016 elsevier inc all rights reserved.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Hanotel2016,

title = {Signatures d'expositions métalliques dans l'ovocyte de xénope},

author = {Julie Hanotel and Sylvain Slaby and Alain Leprêtre and Jean-François Bodart and Sébastien Lemière and Matthieu Marin},

year  = {2016},

date = {2016-01-01},

booktitle = {12ème colloque annuel du Réseau ÉcoBIM, 30 mai-1er juin 2016, Le Havre},

abstract = {Nous faisons actuellement face à la 6ème crise d’extinction de masse touchant l’ensemble de la biodiversité particulièrement le groupe des amphibiens. mieux évaluer l’impact des xénobiotiques sur le cycle de vie des amphibiens est nécessaire pour comprendre leur déclin et mettre en place de nouvelles méthodes de surveillance environnementale. dans cette étude, nous nous proposons d’utiliser un nouvel modèle en écotoxicologie, l’ovocyte de xénope (xenopus laevis), cellule bien connue dans d’autres domaines de la biologie. notre travail se focalise particulièrement sur la maturation ovocytaire (étape de préparation à la ponte et à la fécondation), en identifiant les effets de contaminants métalliques sur diverses voies de signalisation : la voie mapk (principale voie activée au cours de la maturation ovocytaire, le mpf (principal déclencheur de la reprise du cycle cellulaire (phase m)) et la o glcnacylation et la o-glcnac-transférase ogt (voie contrôlant de nombreux processus biologiques ; activée en réponse à divers stress). nos résultats montrent que le cadmium (cdcl2) (1) diminue le taux de maturation, induit (2) de la maturation spontanée, (3) une anomalie de phosphorylation de l’histone h3 (cible du mpf), (4) une augmentation de l’expression de l’ogt et du taux d’o-glcnacylation, sans modifier la voie mapk. de façon intéressante, les mêmes expositions réalisées avec du plomb (pbcl2), n’ont révélé aucun effet sur la réussite de la maturation ni sur les acteurs moléculaires étudiés. ainsi, notre travail met en évidence des réponses différences entre les expositions au cadmium et au plomb, révélant alors des signatures spécifiques dans l’ovocyte de xénope. ces réponses nous permettent d’envisager le développement de nouveaux biomarqueurs et l’utilisation de l’ovocyte de xénope dans l’évaluation de la qualité des milieux aquatiques.},

note = {AFF},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Slaby2016,

title = {Biometric data assessment on Xenopus laevis tadpoles},

author = {Sylvain Slaby and Julie Hanotel and Jean-François Bodart and Sébastien Lemière and Dave Trinel and Alain Leprêtre and Corentin Spriet and Matthieu Marin},

year  = {2016},

date = {2016-01-01},

journal = {Journal of Xenobiotics},

volume = {6},

number = {2},

pages = {33-35},

abstract = {Introduction recent studies stated that 41% of known species of amphibians are threatened.1 indeed, their dramatic population decline is due to environmental conditions, including pollution.2,3 their permeable skin and their close relationship with aquatic compartment, make them particularly sensitive to contamination and stand as strong models in biomonitoring studies.4 consequently, the use of biomarkers in batrachians arose in the 80’s5 : from morphological and anatomical approaches (deformities, gonadic abnormalities) to biochemical measurements (vitellogenin, methallothionein, cyp450).4 for example, embryotoxic and teratogenic damages on larvae were detected with the fetax (frog embryo teratogenesis assay - xenopus) test developed in xenopus laevis. 6-8 in this regard, this specie appears as a very effective laboratory model for assays to dissect actions of chemical contamination. indeed, profusion of data is available in cell cycle, embryology and development fields.9- 11 moreover, our recent studies showed some different sub-individual modifications, especially a decrease of oocytes’ capacity to perform the maturation and the fertilization in presence of cadmium.12,13 here, we propose new methods to quantify the impacts of chemical contaminants on xenopus laevis young tadpoles by recording biometric criteria},

note = {ACLO},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Slaby2016b,

title = {Fécondation et développement du xénope, étapes critiques en milieux contaminés par des métaux ?},

author = {Sylvain Slaby and Julie Hanotel and Alain Leprêtre and Jean-François Bodart and Matthieu Marin and Sébastien Lemière},

year  = {2016},

date = {2016-01-01},

booktitle = {12ème colloque annuel du Réseau ÉcoBIM, 30 mai-1er juin 2016, Le Havre},

note = {ACTI},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Slaby2016a,

title = {Cadmium but not lead exposure affects Xenopus laevis fertilization and embryo cleavage},

author = {Sylvain Slaby and Sébastien Lemière and Julie Hanotel and Arlette Lescuyer and Sylvain Demuynck and Jean-François Bodart and Alain Leprêtre and Matthieu Marin},

year  = {2016},

date = {2016-01-01},

journal = {Aquatic Toxicology},

volume = {177},

pages = {1-7},

abstract = {Among the toxicological and ecotoxicological studies, few have investigated the effects on germ cells, gametes or embryos, while an impact at these stages will result in serious damage at a population level. thus, it appeared essential to characterize consequences of environmental contaminant exposures at these stages. therefore, we proposed to assess the effects of exposure to cadmium and lead ions, alone or in a binary mixture, on early stages of xenopus laevis life cycle. fertilization and cell division during segmentation were the studied endpoints. cadmium ion exposures decreased in the fertilization rates in a concentration-dependent manner, targeting mainly the oocytes. exposure to this metal ions induced also delays or blockages in the embryonic development. for lead ion exposure, no such effect was observed. for the exposure to the mixture of the two metal ions, concerning the fertilization success, we observed results similar to those obtained with the highest cadmium ion concentration},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Slaby2016c,

title = {Cadmium but not Lead affects Xenopus laevis fertilization and embryogenesis},

author = {Sylvain Slaby and Sébastien Lemière and Arlette Lescuyer and Jean-François Bodart and Alain Leprêtre and Matthieu Marin},

year  = {2016},

date = {2016-01-01},

booktitle = {SETAC Europe, 22-26 mai 2016, Nantes},

abstract = {Environmental contaminations have significant effects on biodiversity, especially on amphibians group. this class, containing the highest number of known threatened species, is highly sensitive towards chemical contaminants. despite toxicity, dominance and persistence in the environment of metal contaminants, effects on amphibians remain poorly documented, except through the fetax test. but this assay, use to determine lethal and teratogenic concentrations of solutions, runs with few-days old tadpoles. so effects on gametes, external fertilization and embryogenesis are not considered. whereas these steps are also exposed to environmental contaminations, they’re stills poorly documented yet. in this context and according to previous results of ours showing that x. laevis oocytes maturation was altered by metal chlorides, we examined how cadmium and lead interact with earlier stages of xenopus laevis, from fertilization to 8-cells zygotes. while lead doesn’t disturb the fertilization rates, cadmium decreases these reproductive parameter in a dose dependant manner. similar harmful effects are obtained when these two metals are in mixture at 25 mg/l. whereas more investigations are required, at the sight of these outcomes, we could assume there is no combination effect when cadmium and lead chlorides are in mixture. we also observed that cadmium delays the occurrence of different embryogenic stages. so it disturbed cell division. these observations are in accordance with previous studies analysing from a histological point of view the xenopus oocyte maturation in cadmium contaminated media, where different kinds of atypical structures on the chromosomal formations (pronuclei, ectopic and disorganized spindles) have been reported. cadmium also decreases fertilization whatever the male or the female gametes have been pre-exposed. but the fertilization success is more affected when oocytes are the cd pre-exposed ones. furthermore, no fertilization are able to reach the stage 2 in this condition. our results highlight that cadmium altered xenopus reproduction, affecting fertilization success and embryogenesis, preventing the formation of tadpoles. analysing earlier stages will procured complementary information to the fetax test. moreover oocytes seem to be particularly threatened, and let suggest they could be considered as early and sensitive endpoint (and target) to assess environmental contaminants effects.},

note = {AFF},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Lanier2016,

title = {Combined effect of Cd and Pb spiked field soils on bioaccumulation, DNA damage, and peroxidase activities in Trifolium repens},

author = {Caroline Lanier and Fabien Bernard and Sylvain Dumez and Julie Leclercq-Dransart and Sébastien Lemière and Franck Vandenbulcke and Fabrice Nesslany and Anne Platel and I Devred and Damien Cuny and Annabelle Deram},

year  = {2016},

date = {2016-01-01},

journal = {Environmental Science and Pollution Research},

volume = {23},

number = {2},

pages = {1755-1767},

abstract = {The present study was designed to investigate the combined effects of cd and pb on accumulation and genotoxic potential in white clover (trifolium repens). for this purpose, t. repens was exposed to contaminated soils (2.5–20 mg kg−1 cadmium (cd), 250–2000 mg kg−1 lead (pb) and a mixture of these two heavy metals) for 3, 10 and 56 days. the resulting bioaccumulation of cd and pb, dna damage (comet assay) and peroxidase activities (apox and gpox) were determined. the exposure time is a determinant factor in experiments designed to measure the influence of heavy metal contamination. the accumulation of cd or pb resulting from exposure to the two-metal mixture does not appear to depend significantly on whether the white clover is exposed to soil containing one heavy metal or both. however, when t. repens is exposed to a cd/pb mixture, the percentage of dna damage is lower than when the plant is exposed to monometallic cd. dna damage is close to that observed in the case of monometallic pb exposure. peroxidase activity cannot be associated with dna damage under these experimental conditions},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Bernard2015,

title = {Antioxidant responses of Annelids, Brassicaceae and Fabaceae to pollutants: A review},

author = {Fabien Bernard and Franck Brulle and Sylvain Dumez and Sébastien Lemière and Anne Platel and Fabrice Nesslany and Damien Cuny and Annabelle Deram and Franck Vandenbulcke},

year  = {2015},

date = {2015-01-01},

journal = {Ecotoxicology and Environmental Safety},

volume = {114},

pages = {273-303},

abstract = {Pollutants, such as Metal Trace Elements (MTEs) and organic compounds 

	(polycyclic aromatic hydrocarbons, pesticides), can impact DNA structure 

	of living organisms and thus generate damage. For instance, cadmium 

	is a well-known genotoxic and mechanisms explaining its clastogenicity 

	are mainly indirect: inhibition of DNA repair mechanisms and/or induction 

	of Reactive Oxygen Species (ROS). Animal or vegetal cells use antioxidant 

	defense systems to protect themselves against ROS produced during 

	oxidative stress. Because tolerance of organisms depends, at least 

	partially, on their ability to cope with ROS, the mechanisms of production 

	and management of ROS were investigated a lot in Ecotoxicology as 

	markers of biotic and abiotic stress. This was mainly done through 

	the measurement of enzyme activities The present Review focuses on 

	3 test species living in close contact with soil that are often used 

	in soil ecotoxicology: the worm Eisenia fetida, and two plant species, 

	Trifolium repens (white clover) and Brassica oleracea (cabbage). 

	E. fetida is a soil-dwelling organism commonly used for biomonitoring. 

	T. repens is a symbiotic plant species which forms root nodule with 

	soil bacteria, while B. oleracea is a non-symbiotic plant. In literature, 

	some oxidative stress enzyme activities have already been measured 

	in those species but such analyses do not allow distinction between 

	individual enzyme involvements in oxidative stress. Gene expression 

	studies would allow this distinction at the transcriptomic level. 

 

	A literature review and a data search in molecular database were carried 

	out on the basis of keywords in Scopus, in PubMed and in Genbank™ 

	for each species. Molecular data regarding E. fetida were already 

	available in databases, but a lack of data regarding oxidative stress 

	related genes was observed for T. repens and B. oleracea. By exploiting 

	the conservation observed between species and using molecular biology 

	techniques, we partially cloned missing candidates involved in oxidative 

	stress and in metal detoxification in E. fetida, T. repens and B. 

	oleracea.},

note = {ACLS},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Bernard2015a,

title = {Gene expression analysis of antioxidant responses in Annelids, Brassicaceae and Fabaceae exposed to cadmium and/or lead contaminated soils},

author = {Fabien Bernard and Franck Brulle and Sylvain Dumez and Sébastien Lemière and Anne Platel and Fabrice Nesslany and Damien Cuny and Annabelle Deram and Franck Vandenbulcke},

year  = {2015},

date = {2015-01-01},

booktitle = {SETAC, 3-7 May 2015, Barcelone (Espagne)},

abstract = {Pollutants, such as Metal Trace Elements (MTEs) and organic compounds 

	(polycyclic aromatic hydrocarbons, pesticides), can impact DNA structure 

	of living organisms and thus generate DNA damage. Animal or vegetal 

	cells use antioxidant defense systems to protect themselves against 

	Reactive Oxygen Species (ROS) produced during oxidative stress. The 

	mechanisms of production and management of ROS were investigated 

	a lot in ecotoxicology as unspecific markers. We performed a literature 

	survey of the biochemical responses (enzyme activities) and expression 

	variations of genes encoding proteins involved and/or related to 

	antioxidant response in three species that are often used in ecotoxicology 

	and that live in close contact with soil: the earthworm, Eisenia 

	fetida, the white clover, Trifolium repens and the cabbage, Brassica 

	oleracea. Literature survey and data search in molecular database 

	were carried out on the basis of keywords in Scopus, in PubMed and 

	in Genbank™ for each species (and in related species). In literature, 

	some antioxidant enzyme activities have already been measured in 

	those species but such analysis do not allow distinction of individual 

	enzyme involvements in oxidative stress. Gene expression studies 

	would allow this distinction at the transcriptomic level. By exploiting 

	the conservation observed between species and using molecular biology 

	techniques, we partially cloned and measured the gene expression 

	of many candidates involved and/or related to antioxidant responses 

	in E. fetida, T. repens and B. oleracea. Therefore, analysis of the 

	gene expression level of most effectors involved and/or related to 

	antioxidant responses has been performed in the 3 species exposed 

	in vivo (3, 10 and 56 days) in a well-characterised sub-urban reference 

	soil spiked with cadmium and/or lead at environmental concentrations. 

	The use of gene expression analysis (in combination with metal accumulation 

	analysis) has shown the interest of studying the effects of metal 

	stress in several species. A major interest of this work lies in 

	the measurement of most genes involved and/or related to antioxidant 

	response at the same time and in several species. Indeed, as responses 

	to oxidative stress may be different between species for the same 

	protein family, one may keep in mind that model species should be 

	used in a complementary manner in order to better understand the 

	effects of xenobiotics on exposed organisms.},

note = {AFF},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Jacques2015,

title = {Lipopeptides: an emerging family of biopesticides - Les lipopeptides: une famille emergente de biopesticides Projet interreg phytobio 2010-2013},

author = {Philippe Jacques and François Krier and François Coutte and Sébastien Lemière and Monica Höfte and Marc Ongena and Essaid-Ait Barka and Stephan Dorey and Cédric Jacquard and Philippe Reignault and Béatrice Randoux and Lieven Delanote and Nathalie Cap},

year  = {2015},

date = {2015-01-01},

booktitle = {5th International Conference on Alternative Methods of Crop Protection, 11-13 mars 2015, Lille},

note = {ACTN},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Lemiere2015,

title = {Is Eisenia fetida a suitable species to monitor environmental soil genotoxicants},

author = {Sébastien Lemière and Annabelle Deram and Fabien Bernard and Alain Leprêtre and Franck Vandenbulcke},

year  = {2015},

date = {2015-01-01},

booktitle = {SETAC, 3-7 May 2015, Barcelone (Espagne)},

abstract = {As well as for air and waters, not only chemical analyses have to be taken into account to monitor anthropic contamination of soils. biological responses of exposed organisms should be considered too and particularly for environmental genotoxicants as pahs or metals. it is well-known that studies of soil contaminations are complex. for example, the environmental availability, environmental and toxicological bioavailabilities of present metal contaminants, influenced by soil factors, ageing phenomena, organic matter..., their concomitant presences, and often at low concentrations and then their potential toxic interactions have to be considered. that’s why methods of classical genetic toxicology (indirect testing, unrealistic exposure...) did not seem fully satisfactory. several years ago, we developed an environmental genotoxicity marker with eisenia fetida,the comet assay. it is a technique allowing the evaluation of dna damage (single and double strand breaks, alkali-labile sites) in a cellular population and it was developed on cœlomocytes of this biological model largely used in soil ecotoxicology. in this poster presentation, we will present obtained results in different contexts with this environmental genotoxicty marker: exposures to field-contaminated soils (1), to spiked soils with a mixture of metals (2) and to soils after a remediation treatment (3). in the first work (1), in vivo short-term exposures of e. fetida were conducted using soils sampled in two different areas in the north of france: from the vicinity of a former smelter and from a suburb zone close to a battery plant still in activity. in the second (2), in order to better understand metal interactions, in vivo short to mid-term exposures (3, 10 and 56 days) were performed using an urban-surrounding soil spiked with cadmium or/and lead at concentrations found in french contaminated soils. in the last work (3), in vivo short-term exposures of e. fetida were conducted using a contaminated soil sampled in the field and a reference soil contaminated or not by a mixture of lead, zinc and cadmium. in both soils were added (or not) remediation agents (biochars from two origins) at two concentrations. in all cases, obtained genotoxicity results after these exposures will be detailed. we will try to discuss the interests and limits of our environmental genotoxicity marker and to conclude if it is useful and relevant to monitor anthropic contaminated soils.},

note = {AFF},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Marin2015,

title = {Xenopus laevis oocyte maturation is affected by metal chlorides},

author = {Matthieu Marin and Sylvain Slaby and Guillaume Marchand and Sylvain Demuynck and Noémie Friscourt and Armance Gelaude and Sébastien Lemière and Jean-François Bodart},

year  = {2015},

date = {2015-01-01},

journal = {Toxicology in Vitro},

volume = {29},

number = {5},

pages = {1124-1131},

abstract = {Few studies have been conducted using xenopus laevis germ cells as oocytes, though these cells offer many advantages allowing both electrophysiological studies and morphological examination. our aim was to investigate the effects of metal (cadmium, lead, cobalt and zinc) exposures using cell biology approaches. first, cell survival was evaluated with both phenotypical and electrophysiological approaches. secondly, the effect of metals on oocyte maturation was assessed with morphological observations and electrophysiological recordings. from survival experiments, our results showed that metal chlorides did not affect cell morphology but strongly depolarized x. laevis oocyte resting potential. in addition, cadmium chloride was able to inhibit progesterone-induced oocyte maturation. by contrast, zinc, but also to a lesser extent cadmium, cobalt and lead, were able to enhance spontaneous oocyte maturation in the absence of progesterone stimulation. finally, electrophysiological recordings revealed that some metal chlorides (lead, cadmium) exposures could disturb calcium signaling in x. laevis oocyte by modifying calcium-activated chloride currents. our results demonstrated the high sensitivity of x. laevis oocytes toward exogenous metals such as lead and cadmium. in addition, the cellular events recorded might have a predictive value of effects occurring later on the ability of oocytes to be fertilized. together, these results suggest a potential use of this cellular lab model as a tool for ecotoxicological assessment of contaminated fresh waters.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Slaby2015,

title = {Effects of cadmium on maturation, fertilization and development of Xenopus laevis oocyte},

author = {Sylvain Slaby and Sébastien Lemière and Arlette Lescuyer and Sylvain Demuynck and Jean-François Bodart and Matthieu Marin},

year  = {2015},

date = {2015-01-01},

booktitle = {SETAC, 3-7 May 2015, Barcelone (Espagne)},

abstract = {Through their activities, humans contaminate their environment, by discharging intentionally or not organic or inorganic pollutants. the latter cause adverse effects on the ecosystems and this pollution contribute to the major causes of the global decline of biodiversity, including amphibians. indeed, the aquatic compartments are particularly affected by anthropogenic contaminations. the aim of this work was to highlight the effect of metallic environmental exposure on xenopus laevis oocytes. cadmium (cdcl2), an inorganic compound with natural and mainly anthropogenic sources, such as metallurgy, was chosen. we first focused on cell survival with phenotypical (morphology, pigmentation), physiological (maturation ability) and electrophysiological (resting potential) approaches. then, maturation was assessed in vitro, with morphological (migration and breakdown of the germinal vesicle – gvbd), biochemical (activation of mapk – mitogen activated protein kinase – and mpf – m-phase promoting factor – pathways) and histological (meiotic spindle formation, polar body extrusion) aspects. finally, fertilization and early stages of development (up to one week) were studied. in another work, we demonstrated that chloride metal exposure affects oocyte survival with both morphological and electrophysiological methods. here, oocytes survival was considered by a new physiological method: the ability to undergo maturation after hormonal (progesterone) stimulation i.e. the gain for the oocytes to be competent for fertilization. in a second set of experiments, after cadmium exposures, we demonstrated that mpf pathway (associated with oocyte maturation) is deregulated. fertilization was then assessed when male and female gametes were, together or separately, exposed to cadmium. our data showed that cadmium exposure was responsible for adverse effects in a dose dependent manner on in vitro fertilization rates, development and organogenesis. in addition, our results showed that cadmium preferentially targeted the female gamete rather than spermatozoon, and prevented them to be fertilized. taken together, our results demonstrated that early stages of amphibians could be critical windows of exposure to cadmium.},

note = {AFF},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Slaby2015a,

title = {Xenopus laevis oocyte maturation is affected by metal chlorides},

author = {Sylvain Slaby and Sébastien Lemière and Guillaume Marchand and Sylvain Demuynck and Noémie Friscourt and Armance Gelaude and Jean-François Bodart and Matthieu Marin},

year  = {2015},

date = {2015-01-01},

booktitle = {SETAC 3-7 May 2015, Barcelone (Espagne)},

note = {ACTI},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Vandenbulcke2015b,

title = {Ecotoxicologie des nano-objets : le nano-argent, une nanoparticule d’origine anthropique déjà rejetée dans l’environnement?},

author = {Franck Vandenbulcke and Sébastien Lemière},

year  = {2015},

date = {2015-01-01},

booktitle = {Journée thématique "nano-poudres, nano-materiaux, nano-objets: produits du futur, de la molecule au consommateur", Ecole Centrale de Lille, 29 janvier 2015, Lille},

note = {INV},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Buscaroli2014,

title = {Exploitation of cation exchange for a sustainable depollution of water channel sediments: ecotoxicological assessment, speciation of exchanged metals and water stability of treated sediments},

author = {Enrico Buscaroli and Martina Bonadio and Emanuele Argese and Gloria Falsone and Claudio Marzadori and Luigi Sciubba and Sébastien Lemière and Bertrand Pourrut and Ilaria Braschi},

year  = {2014},

date = {2014-01-01},

booktitle = {Sustainable Remediation Conference 2014, 17-19 September 2014, Ferrara (Italie)},

abstract = {In many countries all over the world, the contamination level of soils is defined by the total content of both inorganic and organic pollutants. as far as the organic pollutants are concerned, this parameter can be considered as an acceptable index of their bioavailability due to their environmental mobility and bioaugmentation inside the leaving organisms. on the contrary, it is of general knowledge that the total content of inorganic pollutants, as heavy metals, cannot be considered responsible for the detrimental effect on soil biota for which only the bioavailable forms should be accounted [1]. a special case is represented by the sediment of water channels collecting and distributing water for agricultural purposes in lands with elevations below sea level. in case these channels are isolated from the river net, they behave like a sink for pollutants coming from the neighbouring fields and the point source pollution (e.g. waste water treatment plants and/or industrial activities) to whom they are eventually linked. the dredging up activities performed on water channel produce high amounts of sediment usually characterized by a pollution which can be considered of medium-low level but still unsafe to be recycled to build channel banks or to be distributed on the surrounding fields. in order to save soil (due to its very fine texture, water channel sediment can be potentially considered as a highly fertile soil type) and to save money (the sediment transport and disposal into waste dump is highly costly), it is of general interest to define sustainable depollution procedures aimed at an in situ restoration of sediments dredged up from water channels. in this study, the sediment of a water channel placed into the north-eastern italy (emilia-romagna region) was sampled at different distances from the output of a municipal waste water treatment plant, chemico-physically characterized and assessed for its organic and inorganic pollutant content. to performe an in situ restoration of sediments, an exchange with calcium ion has been applied directly on the hydrated samples in order to favour displacement kinetics. the speciation of the exchanged heavy metals through sequential extractions helped to define the speciation of the exchanged heavy metals. an ecotoxicological assessment of the treated and control sediments was performed to evaluate the potential harmful effects on the environment. ecotoxicity and genotoxicity of both sediments and pore-water extracted from the sediments were evaluated on the three model organisms: the earthworm eisenia fetida and the plants lolium perene and trifolium repens. effects on survival, growth rate, oxidative stress and dna degradation were determined. finally, the stability of sediments saturated with calcium ion has been evaluated by wet sieving, in order to test the resistance of treated-sediments to water abrasion and compared to the controls. [1] s. tampouris, n. papassiopi, i. paspaliaris. removal of contaminant metals from fine grained soils, using agglomeration, chloride solutions and pile leaching techniques. j. hazard. mat. b84 (2001) 297–319.},

note = {AFF},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Deram2014,

title = {Complémentarité des modèles végétaux et animaux pour le diagnostic de la fraction génotoxique de sols contaminés},

author = {Annabelle Deram and Sébastien Lemière and Fabien Bernard and Franck Marot and Sylvain Dumez and Anne Platel and Fabrice Nesslany and Franck Vandenbulcke and Damien Cuny},

year  = {2014},

date = {2014-01-01},

booktitle = {Colloque des 3èmes Rencontres nationales de la recherche sur les sites et sols pollués, 18-19 novembre 2014, Paris},

note = {ACTN},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Deravel2014,

title = {Mycosubtilin and surfactin are efficient, low ecotoxicity molecules for the biocontrol of lettuce downy mildew},

author = {Jovana Deravel and Sébastien Lemière and François Coutte and François Krier and Nathalie Van-Hese and Max Béchet and Nathanaëlle Sourdeau and Monica Höfte and Alain Leprêtre and Philippe Jacques},

year  = {2014},

date = {2014-01-01},

journal = {Applied Microbiology and Biotechnology},

volume = {98},

number = {14},

pages = {6255–6264},

abstract = {He use of surfactin and mycosubtilin as an eco-friendly alternative to control lettuce downy mildew caused by the obligate pathogen bremia lactucae was investigated. preliminary ecotoxicity evaluations obtained from three different tests revealed the rather low toxicity of these lipopeptides separately or in combination. the ec50 (concentration estimated to cause a 50 % response by the exposed test organisms) was about 100 mg l−1 in microtox assays and 6 mg l−1 in daphnia magna immobilization tests for mycosubtilin and 125 mg l−1 and 25 mg l−1 for surfactin, respectively. the toxicity of the mixture mycosubtilin/surfactin (1:1, w/w) was close to that obtained with mycosubtilin alone. in addition, the very low phytotoxic effect of these lipopeptides has been observed on germination and root growth of garden cress lepidium sativum l. while a surfactin treatment did not influence the development of b. lactucae on lettuce plantlets, treatment with 100 mg l−1 of mycosubtilin produced about seven times more healthy plantlets than the control samples, indicating that mycosubtilin strongly reduced the development of b. lactucae. the mixture mycosubtilin/surfactin (50:50 mg l−1) gave the same result on b. lactucae development as 100 mg l−1 of mycosubtilin. the results of ecotoxicity as well as those obtained in biocontrol experiments indicated that the presence of surfactin enhances the biological activities of mycosubtilin. mycosubtilin and surfactin were thus found to be efficient compounds against lettuce downy mildew, with low toxicity compared to the toxicity values of chemical pesticides. this is the first time that bacillus lipopeptides have been tested in vivo against an obligate pathogen and that ecotoxic values have been given for surfactin and mycosubtilin.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Jacques2014,

title = {Les lipopeptides d'origine microbienne : des substances testées au laboratoire, sous serre et au champ se montrent prometteuses comme agents de biocontrôle aux multiples facettes},

author = {Philippe Jacques and François Krier and Jovana Deravel and François Coutte and Max Béchet and Valérie Leclère and Marlène Chollet and Françoise Coucheney and Corinne Boistel and Sébastien Lemière and Jolien D'aes and et al.},

year  = {2014},

date = {2014-01-01},

journal = {Phytoma},

volume = {672},

pages = {38-42},

note = {APTV},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Bernard2013,

title = {Gene expression analysis of the genes involved in oxidative stress to explain DNA damages in organisms exposed to cadmium/lead contaminated soils},

author = {Fabien Bernard and Sébastien Lemière and Sylvain Dumez and Franck Brulle and Claude Cocquerelle and Anne Platel and Fabrice Nesslany and Damien Cuny and Annabelle Deram and Franck Vandenbulcke},

year  = {2013},

date = {2013-01-01},

booktitle = {SETAC Europe 23rd Annual Meeting, 12-16 May 2013, Glasgow, Scotland (UK)},

note = {AFF},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Deram2013,

title = {Higher plants to asses the genotoxicity of soil},

author = {Annabelle Deram and Fabien Bernard and Sylvain Dumez and Caroline Lanier and Sébastien Lemière and Anne Platel and Fabrice Nesslany and Franck Vandenbulcke and Damien Cuny},

year  = {2013},

date = {2013-01-01},

booktitle = {SETAC Europe 23rd Annual Meeting, 12-16 May 2013, Glasgow, Scotland (UK)},

publisher = {Deram, A., Bernard, F., Dumez, S., Lanier, C., Lemière, S., Platel, A., Nesslany, F., Vandenbulcke, F. and Cuny, D. (2013). . In "SETAC Europe 23rd Annual Meeting, May 2013, Glasgow, Scotland UK”.},

note = {AFF},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Deram2013a,

title = {Complémentarité de modèles végétaux et animaux pour la detection d’effets clastogéniques par le test de comètes},

author = {Annabelle Deram and Sébastien Lemière and Fabien Bernard and Sylvain Dumez and Anne Platel and Fabrice Nesslany and Franck Vandenbulcke and Damien Cuny},

year  = {2013},

date = {2013-01-01},

booktitle = {Rencontres scientifiques de l’Anses, Risques sanitaires liés à la pollution des milieux aériens et hydriques, Restitution du programme national de recherche Environnement Santé Travail, 16 avril 2013, Maisons-Alfort},

abstract = {Objectifs les objectifs de ce programme étaient de mieux apprécier les risques génotoxiques induits par des expositions à des matrices contaminées complexes (sols) et le développement d’outils d’évaluation de la fraction toxique susceptible de porter atteinte à l’adn. la génotoxicité environnementale a été évaluée par le test des comètes permettant la quantification des cassures de l’adn dans des populations cellulaires. ce test a été réalisé, après des expositions aux mêmes sols contaminés, de quatre organismes de niveaux trophiques différents : trifolium repens, brassica oleracea, eisenia fetida et le rat. l’étude concomitante de ces organismes végétaux et animaux devait permettre d’apprécier ainsi la sensibilité et la variabilité des réponses obtenues en termes de détection du caractère génotoxique.},

note = {COM},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Jacques2013,

title = {Novel biosurfactants with biocontrol activity and low ecotoxicity},

author = {Philippe Jacques and J Castera-Guy and Nour-Eddine Chihib and François Coutte and Françoise Coucheney and Jovana Deravel and Patrick Fickers and Monica Höfte and Valérie Leclère and Sébastien Lemière and Alain Leprêtre and Nathalie Van-Hese and Bernard Wathelet},

year  = {2013},

date = {2013-01-01},

booktitle = {9th European Congress of Chemical Engineering and 2nd European Congress of Applied Biotechnology, 21-25 April 2013, The Hague (The Netherlands)},

note = {COM},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Lemiere2013,

title = {Comet assay in higher plants and invertebrate to assess the genotoxicity of anthropic contaminated soils},

author = {Sébastien Lemière and Fabien Bernard and Sylvain Dumez and Fabrice Nesslany and Anne Platel and Damien Cuny and Franck Vandenbulcke and Annabelle Deram},

year  = {2013},

date = {2013-01-01},

booktitle = {SEGH 2013 29th International Conference. 8 juillet 2013, Toulouse},

note = {ACTI},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Lemiere2013b,

title = {Hazards of anthropic contaminated soils: what could we learn from environmental genotoxicity markers?},

author = {Sébastien Lemière and Annabelle Deram and Fabien Bernard and Sylvain Dumez and Fabrice Nesslany and Anne Platel and Marion Delattre and Damien Cuny and Franck Vandenbulcke},

year  = {2013},

date = {2013-01-01},

booktitle = {SETAC Europe 23rd Annual Meeting. 12-16 May 2013, Glasgow, Scotland (UK)},

note = {ACTI},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Lemiere2013c,

title = {The Xenopus oocyte, a reliable tool in ecotoxicology?},

author = {Sébastien Lemière and Guillaume Marchand and Sylvain Demuynck and Matthieu Marin},

year  = {2013},

date = {2013-01-01},

booktitle = {SETAC Europe 23rd Annual Meeting, 12-16 May 2013, Glasgow, Scotland (UK)},

note = {AFF},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Marin2013,

title = {Toxicity of metal chlorides to Xenopus oocytes},

author = {Matthieu Marin and Sébastien Lemière and Guillaume Marchand and Sylvain Demuynck and Jean-François Bodart},

year  = {2013},

date = {2013-01-01},

booktitle = {SEGH 2013 29th International Conference, 8 juillet 2013, Toulouse},

abstract = {All around the world, amphibians have declined dramatically over the last 30 years. this decline was first thought to be caused only by the destruction of aquatic habitats, but it was more recently also recognized as the result of anthropogenic contamination of surface still waters. consequently, although all amphibian species beneficiate of the status of endangered species, intensive works have been performed to increase knowledge on both cellular events occurring during normal development and effects of contaminants on their life cycle. for instance, fetax (frog embryo teratogenesis assay xenopus) assay aimed at determining the impact of pollutants on the development using the model xenopus laevis, a south african frog reared in laboratory conditions. nevertheless, few studies have been conducted using xenopus germ cells as oocytes. though these cells offer many advantages allowing both electrophysiological studies and morphological examination. the aim of this work was to investigate the effects of metal contaminant (cadmium, lead, cobalt and zinc) exposures, at environmental concentrations, on xenopus oocytes, using cell biology approaches. two different goals were followed. first, cell survival was evaluating with both phenotypical and electrophysiological approaches, and secondly, the effect of metals on oocyte maturation was assessed with morphological observations (germinal vesicle breakdown) and electrophysiological recordings (calcium-activated chloride currents). our results show that metal chlorides did not affect cell morphology but strongly depolarized oocytes resting potential. in addition, cadmium chloride was able to inhibit progesterone-induced oocyte maturation in a dose-dependent manner. by contrast, alone, we demonstrated that cadmium as well as zinc, cobalt and lead, are able to enhance oocyte maturation. finally, electrophysiological recordings revealed that metal chloride exposures could disturb calcium signalling in xenopus oocyte by modifying calcium activated chloride currents. our results obtained with metal chlorides, demonstrated that xenopus laevis oocyte could be an innovating lab model in ecotoxicology},

note = {AFF},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}