Publications

@article{Gueguen2009,

title = {Oyster hemocytes express a proline-rich peptide displaying synergistic antimicrobial activity with a defensin},

author = {Yannick Gueguen and Bernard Romestand and Julie Fievet and Paulina Schmitt and Delphine Destoumieux-Garzón and Franck Vandenbulcke and Philippe Bulet and Evelyne Bachère},

year  = {2009},

date = {2009-01-01},

journal = {Molecular Immunology},

volume = {46},

number = {4},

pages = {516-522},

abstract = {A cdna sequence that encodes a 61-amino acid polypeptide precursor with homologies to proline-rich antimicrobial peptides (amps) was identified in the oyster crassostrea gigas. after release of a hydrophobic signal peptide, the resulting 37-amino acid peptide, cg-prp, is composed of an acidic region and a cationic proline-rich region. to evaluate the biological properties of cg-prp, multiple proline-rich peptides corresponding to putative processing of the full-length cg-prp were synthesized. a limited antimicrobial activity was observed for two of them, which also showed strong synergistic antimicrobial activity with cg-def, a defensin from c. gigas. to our knowledge, this is the first evidence of synergy between a defensin and another amp in an invertebrate. by in situ hybridization, the expression of cg-prp was found to be restricted to hemocytes and induced following bacterial challenge. cg-prp transcripts were also detected in hemocytes infiltrating mantle, where cg-def is expressed. additionally, by immunocytochemistry, we showed that cg-prp or one of its variants is present in some hemocytes together with defensins. in conclusion, we described here the first proline-rich amp from mollusk. from our study, it is likely to provide a first line of defense against bacterial invasion by acting through synergy with defensins.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Bernard2009,

title = {Metallic Trace Elements body burden and gene expression analysis of biomarker candidates in Eisenia fetida, during an “intox-detox” protocol performed with field soils},

author = {Fabien Bernard and Franck Brulle and Francis Douay and Sébastien Lemière and Sylvain Demuynck and Alain Leprêtre and Franck Vandenbulcke},

year  = {2009},

date = {2009-01-01},

booktitle = {SETAC Europe 19th Annual Meeting, 31 May-4 June 2009, Göteborg (Suède)},

note = {ACTI},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Bidar2009,

title = {Seasonal and annual variations of the metal uptake and toxicity in Trifolium repens and Lolium perenne growing in a heavy metal contaminated field},

author = {Géraldine Bidar and Guillaume Garçon and Christelle Pruvot and Anthony Verdin and Pirouz Shirali and Francis Douay},

year  = {2009},

date = {2009-01-01},

journal = {Environmental Science and Pollution Research},

volume = {16},

number = {1},

pages = {42-53},

abstract = {Background, aim, and scope the reclamation of nonferrous metal-polluted soil by phytoremediation requires an overall and permanent plant cover. to select the most suitable plant species, it is necessary to study metal effects on plants over the time, thereby checking that metals remain stored in root systems and not transferred to aerial parts. in this purpose, the seasonal and annual variations of metal bioaccumulation, transfer, and phytotoxicity in trifolium repens and lolium perenne grown in a cd-, pb-, and zn-contaminated soil were also studied. materials and methods the experimental site was located near a closed smelter. in spring 2004, two areas were sown with t. repens and l. perenne, respectively. thereafter, the samplings of plant roots and shoots and surrounding soils were realized in autumn 2004 and spring and autumn 2005. the soil agronomic characteristics, the cd, pb, and zn concentrations in the surrounded soils and plant organs, as well as the oxidative alterations (superoxide dismutase [sod], malondialdehyde [mda], and 8-hydroxy-2′-deoxyguanosine [8-ohdg]) in plant organs were carried out. results whatever the sampling period, metal concentrations in soils and plants were higher than background values. contrary to the soils, the fluctuations of metal concentrations were observed in plant organs over the time. bioaccumulation and transfer factors confirmed that metals were preferentially accumulated in the roots as follows: cd>zn>pb, and their transfer to shoots was limited. foliar metal deposition was also observed. the results showed that there were seasonal and annual variations of metal accumulation in the two studied plant species. these variations differed according to the organs and followed nearly the same pattern for the two species. oxidative alterations were observed in plant organs with regard to sod antioxidant activities, mda, and 8-ohdg concentrations. these alterations vary according to the temporal variations of metal concentrations. discussion metal concentrations in surrounded soils and plant organs showed the effective contamination by industrial dust emissions. metals absorbed by plants were mainly stored in the roots. with regard to this storage, the plants seemed to limit the metal transfer to their aerial parts over the time, thereby indicating their availability for metal phytostabilization. aerial deposition was another source of plant exposure to nonferrous metals. despite the occurrence ofmetal-induced oxidative alterations in plant organs, both plant species seemed to tolerate a high metal concentration in soils. conclusions taken together, these results indicated that t. repens and l. perenne were able to form a plant cover on highly cd-, pb-, and zn-polluted soils, to limit the metal transfer to their aerial parts and were relatively metal-tolerant. all these characteristics made them suitable for phytostabilization on metal-contaminated soils. these findings also highlighted the necessity to take into account seasonal and annual variations for a future phytomanagement. recommendations and perspectives in this work, the behavior of plant species grown in metal-polluted soil has been studied during 2 years. obviously, this time is too short to ensure that metals remain accumulated in the root system and few are transferred in aerial parts over the time. it is why regular monitoring should be achieved during more than a decade after the settlement of the plant cover. this work will be completed by the study of the t. repens and l. perenne effects on mobility of metals in order to evaluate the quantities of pollutants which could be absorbed by the biota and transferred to groundwater. bioaccessibility tests could be also realized on polluted soils in order to evaluate the phytostabilization impacts on the exposition risks for humans. keywords bioaccumulation factors . l. perenne . nonferrous metal-contaminated soil . oxidative stress . phytostabilization . seasonal and annual variations . t. repens . transfer factors},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Lemiere2009,

title = {Utilisation de lombricides exposés en microcosms pour évaluer l’intérêt d’agents immobilisants pour la remediation de sols urbains contaminés par les métaux},

author = {Sébastien Lemière and Fabien Grumiaux and Jean-Philippe Godet and Marion Tanguy and Patrick Delplace and Sylvain Demuynck and Franck Brulle and Christelle Pruvot and Franck Vandenbulcke and Francis Douay and Alain Leprêtre},

year  = {2009},

date = {2009-01-01},

booktitle = {2ème Rencontres Nationales de la Recherche sur les Sites & Sols Pollués, ADEME. 20-21 octobre 2009, Paris},

note = {ACTN},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Brulle2009a,

title = {Développement de biomarqueurs d’exposition aux métaux chez un organisme test: l’Annélide Oligochète Eisenia fetida},

author = {Franck Brulle and Claude Cocquerelle and Francis Douay and Sébastien Lemière and Alain Leprêtre and Franck Vandenbulcke},

year  = {2009},

date = {2009-01-01},

booktitle = {2ème Rencontres Nationales de la Recherche sur les Sites & Sols Pollués, ADEME. 20-21 octobre 2009, Paris},

abstract = {Couches supérieures des sols, où les concentrations peuvent être largement supérieures aux teneurs habituelles régionales. les annélides oligochètes sont présents dans ces sols pollués bien qu’ils présentent une sensibilité exacerbée aux métaux. très peu de choses étant connues quant à l’identification et la mise en place des mécanismes de réponse à ces métaux au niveau moléculaire, le travail de caractérisation des candidats biomarqueurs de pollution a été réalisé à partir des cellules immunitaires du ver eisenia fetida, une espèce modèle recommandée en écotoxicologie. deux approches ont été mises en place. une première approche, qualifiée de ciblée et basée sur la conservation phylogénique observée entre espèces, a permis l’identification de plusieurs candidats. une deuxième approche, dite globale et consistant en la construction de banques soustractives a permis d’identifier des gènes dont l’expression est affectée lors d’une exposition à un mélange complexe de métaux. enfin, les candidats biomarqueurs de pollution métallique mis en lumière par les deux approches ont été mesurés sur des animaux exposés à des sols pollués de longue date par des activités métallurgiques. ce travail a été réalisé dans le cadre d’une thèse de doctorat (f. brulle) financée par l’ademe et la région nord-pas-de-calais. 1- premièrement, nous avons cloné et analysé le niveau d’expression de 14 candidats biomarqueurs chez le modèle de laboratoire eisenia fetida exposé in vivo à des sols standards contaminés par le cadmium. les 14 candidats ont été choisis parmi des gènes codant des protéines fortement conservées pour lesquelles des variations d’expression génique ont été rapportées dans la littérature, lors d’exposition de macroinvertébrés à des etms. le niveau d’expression des gènes a été mesuré dans les coelomocytes (cellules immunitaires) puisque la majorité des effecteurs sélectionnés sont connus pour leur implication dans des mécanismes de défense [1].},

note = {ACTN},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Brulle2009b,

title = {Développement de biomarqueurs d'exposition aux métaux chez un organisme test: l'Annélide Oligochète Eisenia fetida},

author = {Franck Brulle and Claude Cocquerelle and Francis Douay and Sébastien Lemière and Alain Leprêtre and Franck Vandenbulcke},

year  = {2009},

date = {2009-01-01},

booktitle = {15ème Colloque National de la Recherche en IUT, CNRIUT 2009. 8-10 juin 2009, Villeneuve d’Ascq},

note = {ACTN},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}