Publications

@article{Pelfrene2008,

title = {Dissolved Cu(II) speciation in unpolluted soil solutions of a planosolic horizon},

author = {Aurélie Pelfrêne and Nathalie Gassama and Daniel Grimaud},

year  = {2008},

date = {2008-01-01},

journal = {Electroanalysis},

volume = {20},

number = {8},

pages = {841-850},

abstract = {Dissolved cu(ii) speciation in unpolluted soil solutions from different horizons was studied using differential pulse anodic stripping voltammetry (dpasv). three sites were selected according to topography and monitored for three years. experiments evidence cu(ii) can be bound by dom (dissolved organic matter) through complexation and/or adsorption reactions. the complexation capacity and the corresponding conditional stability constant were determined for complexation reactions. the adsorbing sites were titrated. a potential binding coefficient was calculated to compare both types of reactions. results show adsorption is less frequent than complexation but exhibits higher binding coefficient.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Gimbert2008,

title = {Long-term responses of snails exposed to cadmium-contaminated soils in a partial life-cycle experiment},

author = {Frédéric Gimbert and Annette De-Vaufleury and Francis Douay and Michaël Coeurdassier and Renaud Scheifler and Pierre-Marie Badot},

year  = {2008},

date = {2008-01-01},

journal = {Ecotoxicology and Environmental Safety},

volume = {70},

pages = {138-146},

abstract = {Juvenile snails were exposed during their growth period to cd-contaminated field and artificial soils and then transferred to uncontaminated soil to assess the sequels of previous exposure on adult reproduction. growth modelling highlighted growth inhibitions of 5% and 10% after 70 and 84 days of exposure to 20 and 100mgcdkg(-1) in artificial soils, respectively. growth disruption was accompanied by a decrease in the clutch number and a 4-week delay in the egg-laying cycle. although it was also contaminated at 20mgcdkg(-1), the contaminated field soil did not lead to detectable effects in snails, suggesting a lower cd bioavailability confirmed by the bioaccumulation analysis. we demonstrated that the 28-day growth test, as advised by the iso-guideline, may not be sufficient to assess sublethal toxic effects of realistically contaminated soils. for this purpose, a life cycle experimental set-up is proposed, allowing a thorough assessment of toxicity during successive life stages.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Griffiths2008,

title = {Soil microbial and faunal responses to herbicide tolerant maize andherbicide in two soils},

author = {B S Griffiths and S Caul and J Thompson and C A Hackett and Jérôme Cortet and Céline Pernin and P H Krogh},

year  = {2008},

date = {2008-01-01},

journal = {Plant and Soil},

volume = {308},

pages = {93-103},

abstract = {A glasshouse experiment was set up to compare processes and organisms in two soils planted with genetically modified (gm) herbicide tolerant (ht) maize treated with appropriate herbicides. this was part of a wider project (ecogen) looking at the consequences of gm cropping systems on soil biology using a tiered approach at laboratory, glasshouse and field scales. soil for the experiment was taken from field sites where the same maize cultivars were grown to allow comparison between results under glasshouse and field conditions. the maize cultivars t25 (gm ht glufosinate-ammonium tolerant), orient (non ht near isogenic control for t25) and monumental (a conventional, non ht variety) were grown in contrasting sandy loam and clay loam soils, half were sprayed with the appropriate herbicide as used in the field and soil samples were taken at the five-leaf and flowering plant growth stage. the main effects on all measured parameters were those of soil type and plant growth stage, with four categories of subsequent interaction: (1) there were no effects of herbicide on plant growth or soil microarthropods: (2) the maize cultivar (but not the gm ht trait) had effects on the decomposition of cotton strips and the nematode community; (3) herbicide application in general altered the community level physiological profile of the microbial community and reduced both soil basal respiration and the abundance of protozoa; and (4) the specific application of glufosinate-ammonium to t25 maize altered soil microbial community structure measured by ester linked fatty acids. the results from this glasshouse experiment support the findings from the field that there are effects of herbicide application on the soil microbial and meso-faunal community but that, compared to other standard agricultural practices, the differences are relatively small},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Pourrut2008,

title = {Potential role of NADPH-oxidase in early steps of lead-induced oxidative burst in Vicia faba roots},

author = {Bertrand Pourrut and Geoffrey Perchet and Jérôme Silvestre and Marie Cecchi and Maritxu Guiresse and Eric Pinelli},

year  = {2008},

date = {2008-01-01},

journal = {Journal of Plant Physiology},

volume = {165},

number = {6},

pages = {571-579},

abstract = {The mechanism of oxidative burst induced by lead in vicia faba excised roots was investigated by luminol-dependent chemiluminescence. results showed that lead triggered a rapid and dose-dependent increase in chemiluminescence production. in this study, specific inhibitors of putative reactive oxygen species (ros) sources were used to determine the mechanism of lead-induced ros generation. this generation was sensitive to dephenylene iodonium (dpi), quinacrine and imidazole, some inhibitors of the nadph-oxidase and not inhibited by other putative ros sources inhibitors. data reported in this work clearly demonstrated the pivotal role of nadph-oxidase-like enzyme in early steps of lead-induced oxidative burst. to investigate the respective implication of calmodulin and protein kinase (pk) in leadinduced nadph-oxidase activation, excised roots were treated with the calmodulin inhibitor w7 or with the pk inhibitor staurosporine. the chemiluminescence generation inhibition by these inhibitors illustrated the role of pk in lead-induced nadph-oxidase activation and revealed a calmodulin-dependent step. using the calcium entry blocker la3+ or different concentrations of calcium in the extracellular medium, our data highlighted the implication of ca2+ channel in leadinduced oxidative burst.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Seignez2008b,

title = {Leaching of lead metallurgical slags and pollutant mobility far from equilibrium conditions},

author = {Nicolas Seignez and Arnaud Gauthier and David Bulteel and Denis Damidot and Jean-Luc Potdevin},

year  = {2008},

date = {2008-01-01},

journal = {Applied Geochemistry},

volume = {23},

pages = {3699-3711},

abstract = {Lead metallurgical slags are partially vitrified materials containing residual amounts of zn, pb, cr, cd and as. these hazardous materials are generally buried on heaps exposed to weathering. in this study, leaching behavior of lead blast furnace slags has been tested using pure water and open flow experiments. it appears that in such far from equilibrium and slightly acidic conditions, the main phase to be altered is the vitreous phase. as for lunar, basaltic and nuclear glasses, alkalis/proton exchanges prevail and lead to the formation of a non-protective altered layer enriched in si, fe and al. the composition of the altered layer is quite constant except for si whose concentration decreases towards the leachate interface. owing to their sizes, micrometric pb droplets are not always totally dissolved at the slag surface. nevertheless, nanometric pb droplets are instantaneously dissolved while a surrounding altered layer is formed. this leads to high pb releases in open flow systems. leachate chemistry and dissolution rates of the vitreous phase are closely comparable to previous leaching tests with basaltic and nuclear glasses in conditions far from equilibrium. moreover, this study confirms that fe is a stable element in such conditions},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Lefebvre2008,

title = {Cathepsin L and cystatin B gene expression discriminates immune cœlomic cells in the leech Theromyzon tessulatum},

author = {Christophe Lefebvre and Franck Vandenbulcke and Béatrice Bocquet and Aurélie Tasiemski and Annie Desmons and Mathilde Vierstraete and Michel Salzet and Claude Cocquerelle},

year  = {2008},

date = {2008-01-01},

journal = {Developmental and Comparative Immunology},

volume = {32},

number = {7},

pages = {795-807},

abstract = {Previous studies evidenced that cystatin b-like gene is specifically expressed and induced in large circulating coelomic cells following bacterial challenge in the leech theromyzon tessulatum. in order to understand the role of that cysteine proteinase inhibitor during immune response, we investigated the existence of members of cathepsin family. we cloned a cathepsin l-like gene and studied its tissue distribution. immunohistochemical studies using anti-cathepsin l and anti-cystatin b antibodies and ultrastructural results demonstrated the presence of three distinct coelomic cell populations: (1) the chloragocytes, which were initially defined as large coelomocytes, (2) the granular amoebocytes and (3) small coelomic cells. among those cells, while chloragocytes contain cystatin b and cathepsin l, granular amoebocytes contain only cathepsin l and the third cell population contains neither cathepsin nor inhibitor. finally, results evidenced that cathepsin l immunopositive granular amoebocytes are chemoattracted to the site of injury and phagocyte bacteria},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Beziat2008,

title = {Gold metallogeny in the Birimian craton of Burkina Faso (West Africa)},

author = {Didier Béziat and Michel Dubois and Pierre Debat and Serge Nikiéma and Stefano Salvi and Francis Tollon},

year  = {2008},

date = {2008-01-01},

journal = {Journal of African Earth Sciences},

volume = {50},

number = {2-4},

pages = {215-233},

abstract = {Primary gold deposits in burkina faso occur in paleoproterozoic birimian belt formations (2.0 ga). mineralization was synchronous with regional metamorphism and deformation, and is either hosted within, or is adjacent to, quartz-bearing veins. these are classical characteristics of epigenetic gold deposits in precambrian metamorphic terranes and permit to classify the mineralized sites from burkina faso as orogenic-type gold deposits. a review of data collected over the past decade by our team permits to recognize two main styles of gold mineralization: (1) quartz-vein hosted; this style occurs in all lithologies, the veins are deformed and gold is principally concentrated within the veins, associated with either sulfides or tourmaline. (2) disseminated; this style occurs exclusively in albitites (and to a lesser extent listvenites) with gold occurring mainly within alteration halos of generally undeformed quartz-albite-carbonate vein. quartz-vein and disseminated styles of mineralization can be associated within the same deposit. albitites and listvenites are alteration products of mainly calc-alkaline igneous rocks of felsic to ultramafic composition, respectively. the predominant alteration assemblage consists of chlorite, albite, carbonate, and pyrite. sulfides occur as fine masses commonly in the alteration halos close to vein margins and consist mainly of pyrite and arsenopyrite, depending on host-rock composition. gold occurs as free native metal and, locally, in form of tellurides, in fissures or as inclusions within pyrite and arsenopyrite. two main populations of fluid inclusions are associated with the gold deposits, independently of the mineralization style: (1) carbonic inclusions consisting of up to 90 mol% co2 (plus n2 and ch4) and (2) aqueous-carbonic fluid inclusions with moderate salinities. interestingly, the disseminated gold style deposits of burkina faso, which have the highest economic potential, show strong similarities with the world-class ashanti deposit, in neighboring ghana.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Lemiere2008,

title = {Eisenia fetida responses to metal immobilizing agents for contaminated soil remediation: a microcosm evaluation},

author = {Sébastien Lemière and Jean-Philippe Godet and Fabien Grumiaux and Patrick Delplace and Sylvain Demuynck and Franck Vandenbulcke and Francis Douay and Alain Leprêtre},

year  = {2008},

date = {2008-01-01},

booktitle = {SETAC Europe 18th Annual Meeting. 25-29 May 2008, Krakow (Pologne)},

note = {ACTI},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Bidar2008,

title = {Changes in fatty acid composition and content of two plants (Lolium perenne and Trifolium repens) grown during 6 and 18 months in a metal (Pb, Cd, Zn) contaminated field},

author = {Géraldine Bidar and Anthony Verdin and Guillaume Garçon and Christelle Pruvot and Frédéric Laruelle and Anne Grandmougin-Ferjani and Francis Douay and Pirouz Shirali},

year  = {2008},

date = {2008-01-01},

journal = {Water Air and Soil Pollution},

volume = {192},

number = {1-4},

pages = {281-291},

abstract = {The aim of this in situ study was to investigate the fatty acid (fa) composition and content in roots and shoots of lolium perenne and trifolium repens, grown under heavy metal stress (cd, pb, zn). the composition of fa was quite similar for the two plants and the two organs; main fa were palmitic acid (c16:0), oleic acid (c18:1), linoleic acid (c18:2) and linolenic acid (c18:3). for both plants, the major fa that characterized the roots was c18:2 whereas c18:3 was the prominent fa in shoots. for the first sampling (s1), in the roots of l. perenne and t. repens, polyunsaturated fatty acids (pufa) were affected by metal contamination while, in the second sampling (s2), pufa were affected in the shoots of the two plants. this alteration of pufa was well correlated with the bioaccumulation factor of metals which decreased in roots and increased in shoots with the time. moreover, a positive correlation was found between the pufa decrease and the malondialdehyde (mda) content, indicating the occurrence of a lipid peroxidation induced by the metal stress},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Lemiere2008a,

title = {An indoor microcosm study with zebra mussels of metalcontaminated sediment resupension},

author = {Sébastien Lemière and Didier Técher and Patrick Delplace and C Gilliers and Sylvain Demuynck and Franck Vandenbulcke and Alain Leprêtre},

year  = {2008},

date = {2008-01-01},

booktitle = {SETAC Europe 18th Annual Meeting, 25-29 May 2008, Krakow (Poland)},

note = {ACTI},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Brulle2008,

title = {Identification and expression profile of gene transcripts differentially expressed during metallic exposure in Eisenia fetida coelomocytes},

author = {Franck Brulle and Claude Cocquerelle and Guillaume Mitta and Vincent Castric and Francis Douay and Alain Leprêtre and Franck Vandenbulcke},

year  = {2008},

date = {2008-01-01},

journal = {Developmental and Comparative Immunology},

volume = {32},

pages = {1441-1453},

abstract = {The aim of this work was to identify in eisenia fetida genes whose expression are regulated following exposure to a complex mixture of metallic trace elements (mte) representative of a highly polluted smelter soil. suppression subtractive hybridization (ssh) was used to construct cdna libraries enriched in up- or down-regulated transcripts in the immunecirculating cells of the coelomic cavities, namely coelomocytes, from worms exposed to metallic pollution. among 1536 ssh-derived cdna clones sequenced, we identified 764 unique ests of which we selected 18 candidates on the basis of their redundancy. these selected candidates were subjected to a two-step validation procedure based on the study of their expression level by real-time pcr. the first step consisted in measuring the expression of the 18 candidates in worms exposed to artificial contaminated soil. the second step consisted in measuring the expression in animals exposed to a ‘‘naturally’’ contaminated soil sampled close to a smelter. both steps allowed us to highlight 3 candidates that are strongly induced in worms exposed to a smelter polluted soil. these candidates are: the well-known mte-induced cd–metallothionein and 2 original biomarkers, lysenin, and a transcript, which cloning of the complete coding sequence identified as the coactosin-like protein.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Brulle2008b,

title = {Identification and expression profile of gene transcripts differentially expressed during metallic exposure in Eisenia fetida},

author = {Franck Brulle and Claude Cocquerelle and Guillaume Mitta and Vincent Castric and Francis Douay and Alain Leprêtre and Franck Vandenbulcke},

year  = {2008},

date = {2008-01-01},

booktitle = {SETAC Europe 18th Annual Meeting, 25-29 May 2008, Krakow (Poland)},

note = {ACTI},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Brulle2008c,

title = {Identification and analysis of the expression profiles of new biomarker candidates to gain insight the immune response to a metallic stress},

author = {Franck Brulle and Claude Cocquerelle and Guillaume Mitta and Sébastien Lemière and Alain Leprêtre and Franck Vandenbulcke},

year  = {2008},

date = {2008-01-01},

booktitle = {SETAC Europe 18th Annual Meeting. 25-29 May 2008, Krakow (Pologne)},

note = {COM},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Brulle2008a,

title = {CDNA cloning and expression analysis of Eisenia fetida (Annelida:Oligochaeta) phytochelatin synthase under cadmium exposure},

author = {Franck Brulle and Claude Cocquerelle and Atta-Nda Wamalah and Andrew-John Morgan and Peter Kille and Alain Leprêtre and Franck Vandenbulcke},

year  = {2008},

date = {2008-01-01},

journal = {Ecotoxicology and Environmental Safety},

volume = {71},

number = {1},

pages = {47-55},

abstract = {Metallothioneins (mts) are central to trace metal homeostasis and detoxification throughout biological systems. prokaryotes, plants, and fungi utilize both gene encoded cysteine-rich polypeptides (classically designated class i and ii mts) and enzymatically synthesized cysteinerich peptides (classically designated class iii mts or phytochelatins). in contrast, although gene encoded mts are ubiquitous in animal species the identification of a functional phytochelatin synthase in the nematode caenorhabditis elegans, a representative member of the ecdysozoa, provided the first evidence for these metal-binding peptides in animals. by exploiting the conservation observed between species we have been able to clone and transcriptionally characterize a phytochelatin synthase from the immune cells of the earthworm eisenia fetida, the first evidence for its presence in a phylum belonging to the lophototrochozoa. the complete coding sequence of this enzyme was determined and the phylogenetic relationship to plant, yeast and nematode enzymes elucidated. temporal- and dose-profiling of the transcriptional regulation of phytochelatin synthase and mt in response to cadmium was performed by using real-time pcr.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}