Publications

@conference{Hedde2010,

title = {Recherche d’outils de surveillance, de caractérisation et d’évaluation des risques des sols},

author = {Mickaël Hedde and Antonio Bispo and Daniel Cluzeau and Jérôme Cortet and Francis Douay and Laurence Galsomies and Cécile Grand and Sabine Houot and Corinne Leyval and Benjamin Pauget and Nuria Ruiz-Camacho and Erwin Ulrich and Franck Vandenbulcke and Annette de-Vaufleury and Cécile Villenave and Guénola Pérès},

year  = {2010},

date = {2010-01-01},

booktitle = {Programme ADEME - Bioindicateur II : bio-indicateurs basés sur la faune du sol. Colloque de la Société Française d’Ecotoxicologie Fondamentale et Appliquée},

note = {ACTN},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Homa2010,

title = {Metal-specific effects on metallothionein gene induction and riboflavin content in coelomocytes of Allolobophora chlorotica},

author = {Joanna Homa and Malgorzata Klimek and Jerzy Kruk and Claude Cocquerelle and Franck Vandenbulcke and Barbara Plytycz},

year  = {2010},

date = {2010-01-01},

journal = {Ecotoxicology and Environmental Safety},

volume = {73},

number = {8},

pages = {1937-1943},

abstract = {Metal pollution affects earthworm coelomocytes, including their differential counts, riboflavin content and metallothioneins (mt) involved in metal homoeostasis and detoxification. the present work shows effects of ni, cu, zn, cd, and pb at the same molarity (1 mm) on coelomocytes of allolobophora chlorotica after 2-day worm dermal exposure to metal chlorides. numbers of coelomocytes/eleocytes extruded by electric shock and amounts of riboflavin in coelomocyte lysates were significantly decreased in cu-exposed worms, less diminished in response to ni, zn, cd, and unaffected by pb. in sharp contrast, real-time pcr revealed a very strong (272 fold) mt-mrna induction in response to cd only. the induction was very low in response to zn, cu, pb, and ni ions (2.6, 2.1, 1.4, and 1.3-fold, respectively). in conclusion, decreased cell counts and riboflavin content are molecular biomarkers of cu exposure while induction of mt-mrna is a molecular biomarker of worm cd exposure},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Alary2010,

title = {Factorial kriging analysis as a tool for explaining the complex spatial distribution of metals in sediments},

author = {Claire Alary and Hélène Demougeot-Renard},

year  = {2010},

date = {2010-01-01},

journal = {Environmental Science and Technology},

volume = {44},

number = {2},

pages = {593-599},

abstract = {Rivers flowing through urbanized and industrial areas are usually greatly damaged by anthropogenic activities discharging contaminants. characterizing the spatial distribution of pollutants in sediments is of high importance for selecting a suitable remediation operation, but is a complex task because this spatial variability is the result of various physical and chemical mechanisms occurring at different scales. factorial kriging analysis (fka)wasappliedondata collected inacanalized river (scarpe, france) for that purpose, because this geostatistical technique allows to decompose a given variable into components of different spatial correlations and map them separately. this decomposition is meaning ful provided that it can be related to physical phenomena occurring at the identified spatial scales.fka applied to cd and zn concentrations in sediments of the scarpe river proved to be effective, allowing their mapping to be decomposedin a first maprelated to a short range spatial correlation corresponding to hot spots interpreted as the impact of industrial and urban inputs located along the canal, and a second map related to a long-range spatial variability associated with long pollutant plumes interpreted as the effect of one major upstream pollutant input.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Roussel2010,

title = {Cd, Pb and Zn oral bioaccessibility of urban soils contaminated in the past by atmospheric emissions from two lead and zinc smelters},

author = {Hélène Roussel and Christophe Waterlot and Aurélie Pelfrêne and Christelle Pruvot and Muriel Mazzuca and Francis Douay},

year  = {2010},

date = {2010-01-01},

journal = {Archives of Environmental Contamination and Toxicology},

volume = {58},

number = {4},

pages = {945-954},

abstract = {Ingestion of dust or soil particles could pose a potential health risk due to long-term metal trace element (mte) exposure. twenty-seven urban topsoil samples (kitchen garden and lawn) were collected and analyzed for cd, pb and zn using the unified bioaccessibility research group of europe (barge) method (ubm) test to estimate the human bioaccessibility of these elements. the quantities of cd, pb and zn extracted from soils indicated, on average, 68, 62 and 47% bioaccessibility, respectively, in the gastric phase and 31, 32 and 23% bioaccessibility, respectively, in the gastro-intestinal phase. significant positive correlations were observed between concentrations extracted with ubm and total mte contents. stepwise multiple regression analysis showed that human bioaccessibility was also affected by some physico-chemical soil parameters (i.e. total nitrogen, carbonates, clay contents and ph). the unified test presents some valuable data for risk assessment. indeed, the incorporation of oral bioaccessible concentrations into risk estimations could give more realistic information for health risk assessment.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Baize2010,

title = {Les éléments en traces dans les sols agricoles du Nord-Pas-de-Calais. I. Étude et cartographie des teneurs des horizons de surface},

author = {Denis Baize and Francis Douay and Estelle Villanneau and Hocine Bourennane and Thibault Sterckeman and Henri Ciesielski and Dominique King},

year  = {2010},

date = {2010-01-01},

journal = {Etude et Gestion des Sols},

volume = {17},

number = {3-4},

pages = {213-237},

abstract = {La base de données utilisée pour la présente étude rassemble des populations d’analyses de sols provenant de cinq sources correspondant chacune à une stratégie d’échantillonnage différente et à un objectif distinct. au total, 3 929 analyses réalisées entre 1990 et 2007 ont été collectées : elles provenaient presque exclusivement d’horizons de surface de sols agricoles. outre les concentrations en etm, les principales caractéristiques agro-pédologiques et les coordonnées géographiques, la base de données contient également des informations sur l’occupation du sol et sur la nature du matériau parental. dans le nord -pas-de-calais affleurent des roches sédimentaires variées, souvent recouvertes par des dépôts éoliens limoneux, mais tous ces matériaux montrent des teneurs naturelles faibles en etm. les différentes stratégies d’échantillonnage se sont assez bien complétées et ont permis une bonne couverture du territoire régional et des diverses causes de contaminations anthropiques : aussi bien les sources diffuses que celles très ponctuelles. ont été réalisés divers traitements tels que : statistiques régionales descriptives ; examen de la localisation des valeurs anomaliques ; cartographies mono-élémentaires par krigeage ; vision multi-élémentaire grâce à un indice numérique additif très simple. un fort contraste apparaît entre des secteurs fortement influencés par des contaminations voire des pollutions multimétalliques (zones riveraines de l’agglomération lilloise, environs immédiats de grands sites industriels) et les zones rurales qui ne montrent pas de contaminations nettes à l’exception du cadmium apporté par la fertilisation phosphatée. outre une vision spatiale des teneurs pour les 8 principaux éléments traces, les traitements ont permis d’avoir une bonne connaissance statistique des teneurs usuelles en stratifiant par matériaux parentaux et par petites régions naturelles. ces « teneurs agricoles habituelles » (tah) ainsi stratifiées peuvent être correctement estimées en considérant toutes les valeurs comprises entre le percentile 10 et le percentile 90. ces tah peuvent être utilisées comme valeurs de référence plus appropriées que de simples statistiques calculées à l’échelon régional ou départemental, tout particulièrement dans le cadre de l’interprétation de l’état des milieux ou pour proposer aux services santé-environnement des agences régionales de santé des valeurs seuils de sélection pour prendre en compte tel ou tel élément trace dans une évaluation des risques sanitaires},

note = {ACLN},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Seignez2010,

title = {Development of plant roots network in polluted soils: An X-ray computed microtomography investigation},

author = {Nicolas Seignez and Arnaud Gauthier and Florias Mees and Celia Brunel and Jean-Luc Potdevin},

year  = {2010},

date = {2010-01-01},

journal = {Water Air and Soil Pollution},

volume = {209},

number = {1-4},

pages = {199-207},

abstract = {X-ray computed microtomography is a non-destructive technique which allows to study in 3d the texture of a solid material and of great interest for non-homogeneous ones. coupled with image analyses software, the soil properties visually determined can be easily supported by statistics. this work proposes to study the root development of arabidopsis halleri growing on different soils polluted with metallurgical slag and dredging sludge. the 3d images reveal that the wastes are not homogeneous and pollutants are concentrated in some small regions randomly spread. they often correspond to lead oxide phases in slags and to ore phases as blende in sludge. their descriptions have also been supported by statistics issued from the image analysis software, aphelion. additional result deals with the capacity of x-ray computed microtomography for primary and secondary rhizosphere in situ analysis which can be measured precisely},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Bernard2010,

title = {Metallic trace element body burdens and gene expression analysis of biomarker candidates in Eisenia fetida, using an ''exposure/depuration'' experimental scheme with field soils},

author = {Fabien Bernard and Franck Brulle and Francis Douay and Sébastien Lemière and Sylvain Demuynck and Franck Vandenbulcke},

year  = {2010},

date = {2010-01-01},

journal = {Ecotoxicology and Environmental Safety},

volume = {73},

number = {5},

pages = {1034-1045},

abstract = {Smelting plant activities lead to the accumulation of metal trace elements (mtes) in soils. the presence of high concentrations of mtes can generate an environmental stress likely to affect macroinvertebrates living in close soil contact such as the annelida oligochaeta. eisenia fetida, an ecotoxicologically important test species, was successively exposed to two field soils: (1) a highly contaminated agricultural topsoil collected near the former smelter metaleurop nord (noyelles-godault, france) which contaminated surrounding soils by its atmospheric emissions [exposure phase], and then (2) a slightly contaminated topsoil from an urban garden located in the conurbation of lille (wambrechies) [depuration phase]. two analyses were performed during each phase. firstly, the gene expression levels of four biomarker candidates identified in previous studies were analyzed in e. fetida coelomocytes. these candidates are cd-metallothionein, phytochelatin synthase, coactosin-like protein and lysenin. secondly, the body burdens of the following elements cd, pb, zn, cu, fe, ca, and p were measured. moreover, both analyses were also performed in lumbricus rubellus, an annelid species collected from the two tested soil-originating sites. analysis of gene expression and mte body burdens in both species are discussed to: (1) evaluate expression biomarkers; (2) gain insight the detoxification processes and the long-term response to a metallic stress and (3) compare the responses observed in a test species (e. fetida) with the responses of a field species (l. rubellus). (c) 2010 elsevier inc. all rights reserved.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Lemiere2010b,

title = {The GeDSeT project: (constitution of a decision support tool (DST) for the management and material recovery of waterways sediments in Belgium and Northern France)},

author = {Bruno Lemière and Pascale Michel and Jérôme Jacob and Laurence Haouche and Claire Alary and Agnès Laboudigue and Hervé Bréquel and B Hazebrouck},

year  = {2010},

date = {2010-01-01},

booktitle = {WASCON 2012 - towards effective, durable and sustainable production and use of alternative materials in construction, May 2012, Gothenburg (Suède)},

volume = {57},

pages = {9pp},

note = {ACTI},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Lemiere2010a,

title = {Indoor microcosm studies for ecotoxicological evaluation of metal contaminated soil remediation agents},

author = {Sébastien Lemière and Jean-Philippe Godet and Marion Tanguy and Fabien Grumiaux and Régine Leroux and Franck Vandenbulcke and Sylvain Demuynck and Céline Pernin and Christelle Pruvot and Patrick Delplace and Francis Douay and Alain Leprêtre},

year  = {2010},

date = {2010-01-01},

booktitle = {SETAC Europe 20th Annual Meeting. 23-27 May 2010, Seville (Espagne)},

note = {ACTI},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Bourennane2010,

title = {Mapping of anthropogenic trace elements inputs in agricultural topsoil from Northern France using enrichment factors},

author = {Hocine Bourennane and Francis Douay and Thibault Sterckeman and Estelle Villanneau and Henri Ciesielski and Dominique King and Denis Baize},

year  = {2010},

date = {2010-01-01},

journal = {Geoderma},

volume = {157},

number = {3-4},

pages = {165-174},

abstract = {Anthropogenic trace element contamination of soils in the densely populated and industrialized nord-pas de calais region (france) was quantified using enrichment factors (efs). total concentrations of al and 18 trace elements (as, bi, cd, co, cr, cu, hg, in, mn, mo, ni, pb, sb, se, sn, tl, v, and zn) were determined in 252 sampling sites both in topsoil and deep horizons of soils developed from sedimentary materials. thus, at each sampling site an enrichment factor was computed for each of the 18 trace elements as the ratio between total concentrations of the trace element in topsoil and deep horizons in the same soil pit, normalized by concentrations of al measured in the same horizons and the same pit. to estimate ef values at unsampled locations and then to assess the spatial magnitude of anthropogenic trace element contamination over the whole study area (12400 km(2)), variography analysis was used to quantify the spatial structure of each ef. results have shown that values of 8 efs are spatially correlated. these efs were thus mapped using the sequential gaussian simulation technique. one hundred horizon samples from 50 supplementary sites were analysed for the structured trace elements and al contents and used for the mapping validation. results of the validation based on the accuracy plots and goodness statistics (g and d) allowed us to conclude that ef maps exhibited high certainty in regard to the validation data set. in addition, results have shown that e-type estimates were accurate when they are used to estimate the measured ef value of the validation data set. accordingly, these maps were used to discuss the origin of patches of surface horizon enrichment displaying an exogenous input of the trace elements investigated. the whole study area under discussion is strongly enriched with cadmium, originating from both industrial and agricultural activities. surface horizons also exhibit locally high levels of pb and zn enrichment, mainly around well-known former or current industrial sites. however. pb and zn enriched surface horizons are far less widespread than those enriched in cd. higher cu, bi and sn ef values were observed in the northern part of the region studied than in the southern part. the dissimilarity underlined a rather diffuse contamination from the urbanized zone in the north to the rural zone in the south of the region. the enrichment of surface horizons by in and tl seems insignificant. some unstructured efs were attributes to point-source enrichments. (c) 2010 elsevier b.v. all rights reserved.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Brulle2010a,

title = {Identification and analysis of the expression profiles of new biomarker candidates in Eisenia fetida: A transcriptomic approach},

author = {Franck Brulle and Claude Cocquerelle and Sébastien Lemière and Francis Douay and Alain Leprêtre and Franck Vandenbulcke},

year  = {2010},

date = {2010-01-01},

booktitle = {SETAC Europe 20th Annual Meeting. 23-27 May 2010, Seville (Espagne)},

note = {COM},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Thevenot2010,

title = {Fate of lignins in soils: A review},

author = {Mathieu Thevenot and Marie-France Dignac and Cornelia Rumpel},

year  = {2010},

date = {2010-01-01},

journal = {Soil Biology & Biochemistry},

volume = {42},

number = {8},

pages = {1200-1211},

abstract = {Lignins are amongst the most studied macromolecules in natural environments. during the last decades, lignins were considered as important components for the carbon cycle in soils, and particularly for the carbon storage. thus, they are an important variable in many soil plant models such as century and rothc, and appeared determinant for the estimation of the soil organic matter (som) pool-size and its stabilization. recent studies challenged this point of view. the aim of this paper was to synthesise the current knowledge and recent progress about quantity, composition and turnover of lignins in soils and to identify variables determining lignin residence time. in soils, lignins evolve under the influence of various variables and processes such as their degradation or mineralization, as well as their incorporation into som. lignin-derived products obtained after cuo oxidation can be used as environmental biomarkers, and also vary with the degree of degradation of the molecule. the lignin degradation is related to the nature of vegetation and land-use, but also to the climate and soil characteristics. lignin content of som decreases with decreasing size of the granulometric fractions, whereas its level of degradation increases concomitantly. many studies and our results suggest the accumulation and potential stabilization of a part of lignins in soils, by interaction with the clay minerals, although the mechanisms remain unclear. lignin turnover in soils could be faster than that of the total som. different kinetic pools of lignins were suggested, which sizes seem to be variable for different soil types. the mechanisms behind different degradation kinetics as well as their potential stabilization behaviour still need to be elucidated. (c) 2010 elsevier ltd. all rights reserved.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Brulle2010b,

title = {Transcriptomic underpinning of toxicant-mediated physiological function alterations in three terrestrial invertebrate taxa: A review},

author = {Franck Brulle and Andrew-John Morgan and Claude Cocquerelle and Franck Vandenbulcke},

year  = {2010},

date = {2010-01-01},

journal = {Environmental Pollution},

volume = {158},

number = {9},

pages = {2793-2808},

abstract = {Diverse anthropogenic activities often lead to the accumulation of inorganic and organic residues in topsoils. biota living in close contact with contaminated soils may experience stress at different levels of biological organisation throughout the continuum from the molecular-genetic to ecological and community levels. to date, the relationship between changes at the molecular (mrna expression) and biochemical/physiological levels evoked by exposures to chemical compounds has been partially established in a limited number of terrestrial invertebrate species. recently, the advent of a family of transcriptomic tools (e.g. real-time pcr, subtractive suppressive hybridization, expressed sequence tag sequencing, pyro-sequencing technologies, microarray chips), together with supporting informatic and statistical procedures, have permitted the robust analyses of global gene expression changes within an ecotoxicological context. this review focuses on how transcriptomics is enlightening our understanding of the molecular-genetic responses of three contrasting terrestrial macroinvertebrate taxa (nematodes, earthworms, and springtails) to inorganics, organics, and agrochemicals},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}